Search results for: store-operated calcium entry
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 263

Search results for: store-operated calcium entry

113 Laboratory Investigations on Mechanical Properties of High Volume Fly Ash Concrete and Composite Sections

Authors: Aravindkumar B. Harwalkar, S. S. Awanti

Abstract:

Use of fly ash as a supplementary cementing material in large volumes can bring both technological and economic benefits for concrete industry. In this investigation mix proportions for high volume fly ash concrete were determined at cement replacement levels of 50%, 55%, 60% and 65% with low calcium fly ash. Flexural and compressive strengths of different mixes were measured at ages of 7, 28 and 90 days. Flexural strength of composite section prepared from pavement quality and lean high volume fly ash concrete was determined at the age of 28 days. High volume fly ash concrete mixes exhibited higher rate of strength gain and age factors than corresponding reference concrete mixes. The optimum cement replacement level for pavement quality concrete was found to be 60%. The consideration of bond between pavement quality and lean of high volume fly ash concrete will be beneficial in design of rigid pavements.

Keywords: Keywords—Composite section, Compressive strength, Flexural strength, Fly ash.

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112 Inulin and Fructooligosaccharides Incorporated Functional Fruit Bars

Authors: P.Megala, T.V.Hymavathi

Abstract:

Papaya and banana bars were developed incorporating inulin (IN) and fructooligosaccharides (FOS) (Liquid and Powder form) in various proportions. The control bars were standardized using 70% fruit pulp, 30% sugar, 0.3% citric acid while the treated bars were standardized with 70% fruit pulp, 15% sugar, 15% of IN and FOS and 0.3% citric acid. Among the various proportions tested, papaya bars with 90% FOS (Powder) + 10% IN and banana bars with 90% FOS (liquid) + 10% IN were sensorially best accepted. The study revealed that addition of IN and FOS improved the sensory scores. The Physico-chemical and proximatecomposition analysis revealed slight changes in brix°, total sugars, reducing sugars, nonreducing sugars, moisture, protein, fat, vitamin C, ash, iron, zinc, calcium and crude fibre between control and treated fruit bars. Further the glycemic index of papaya bar was reduced from 65 to 54 when treated with FOS and IN.

Keywords: Banana, fructooligosaccharides, functional fruit bars, inulin, papaya

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111 Understanding ICT Behaviors among Health Workers in Sub-Saharan Africa: A Cross-Sectional Study for Laboratory Persons in Uganda

Authors: M. Kasusse, M. Rosette, E. Burke, C. Mwangi, R. Batamwita, N. Tumwesigye, S. Aisu

Abstract:

A cross-sectional survey to ascertain the capacity of laboratory persons in using ICTs was conducted in 15 Ugandan districts (July-August 2013). A self-administered questionnaire served as data collection tool, interview guide and observation checklist. 69 questionnaires were filled, 12 interviews conducted, 45 HC observed. SPSS statistics 17.0 and SAS 9.2 software were used for entry and analyses. 69.35% of participants find it difficult to access a computer at work. Of the 30.65% who find it easy to access a computer at work, a significant 21.05% spend 0 hours on a computer daily. 60% of the participants cannot access internet at work. Of the 40% who have internet at work, a significant 20% lack email address but 20% weekly read emails weekly and 48% daily. It is viable/feasible to pilot informatics projects as strategies to build bridges develop skills for e-health landscape in laboratory services with a bigger financial muscle.

Keywords: ICT Behavior, Clinical Laboratory persons, Sub-Saharan Africa, Uganda.

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110 Mineral and Some Physico-Chemical Composition of 'Karayemis' (Prunus laurocerasus L.) Fruits Grown in Northeast Turkey

Authors: İsmail Hakkı Kalyoncu, Nilda Ersoy, Ayşe Yalcın Elidemir, Cansu Dolek

Abstract:

Some physico-chemical characteristics and mineral composition of 'Karayemis' (Prunus laurocerasus L.) fruits which grown naturally in Norteast Turkey was studied. 28 minerals ( Al, Mg, B, Mn, Co, Na, Ca, Ni, Cd, P, Cr, Pb, Cu, S, Fe, Zn, K, Sr, Li, As, V, Ag, Ba, Br, Ga, In, Se, Ti) were analyzed and 19 minerals were present at ascertainable levels. Karayemis fruit was richest in potassium (7938.711 ppm), magnesium (1242.186 ppm) and calcium (1158.853 ppm). And some physico-chemical characteristics of Karayemis fruit was investigated. Fruit length, fruit width, fruit thickness, fruit weight, total soluble solids, colour, protein, crude ash, crude fiber, crude oil values were determined as 2.334 cm, 1.884 cm, 2.112 cm, 5.35 g, 20.1 %, S99M99Y99, 0.29 %, 0.22 %, 6.63 % and 0.001 %, respectively. The seed of fruit mean weight, length, width and thickness were found to be 0.41 g, 1.303 cm, 0.921 cm and 0.803, respectively.

Keywords: Prunus laurocerasus L., physico-chemical properties, nutritional properties.

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109 A Comparative Study on Survival and Growth of Larvivorous Fish, Rasbora daniconius, Puntius ticto, and Puntius Conchonius

Authors: Lavkush Kumar Brahman, Ramesh Chandra

Abstract:

Experiments were carried out on the survival and growth of Rasbora daniconius, Puntius ticto and Puntius conchonius. The motivation of the study was to obtain information for growing the fish on a commercial scale for their use as biological control agents against mosquito larvae. The effects of temperature, total hardness, DO, pH and feed on the growth of fish were also investigated. Excessive value of total hardness was found because very rich calcium ion is present in Chitrakoot area. There was significant increases in growth rates of fish as temperature was increased from 280C to 300C. Further increases in temperature up to 320C, did not further affect growth. The positive and highly significant correlations 0.991488, 0.9581 and 0.9935 were found between length and weight of P. ticto, P. conchonius and R. daniconius respectively. The regression was significant at 5% level of probability.

Keywords: Indigenous fish, DO, larvae, mosquito, pH, Temperature, total hardness.

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108 Viability of Eggshells Ash Affecting the Setting Time of Cement

Authors: Fazeera Ujin, Kamran Shavarebi Ali, Zarina Yasmin Hanur Harith

Abstract:

This research paper reports on the feasibility and viability of eggshells ash and its effects on the water content and setting time of cement. An experiment was carried out to determine the quantity of water required in order to follow standard cement paste of normal consistency in accordance with MS EN 196-3:2007. The eggshells ash passing the 90µm sieve was used in the investigation. Eggshells ash with percentage of 0%, 0.1%, 0.5%, 1.0%, 1.5% and 2.0% were constituted to replace the cement. Chemical properties of both eggshells ash and cement are compared. From the results obtained, both eggshells ash and cement have the same chemical composition and primary composition which is the calcium compounds. Results from the setting time show that by adding the eggshells ash to the cement, the setting time of the cement decreases. In short, the higher amount of eggshells ash, the faster the rate of setting and apply to all percentage of eggshells ash that were used in this investigation. Both initial and final setting times fulfill the setting time requirements by Malaysian Standard. Hence, it is suggested that eggshells ash can be used as an admixture in concrete mix.

Keywords: Construction Materials, Eggshells Ash, Solid Waste, Setting Time.

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107 Geochemistry of Coal Ash in the Equatorial Wet Disposal System Environment

Authors: Kolay P. K., Singh H.

Abstract:

The coal utilization in thermal power plants in Malaysia has increased significantly which produces an enormous amount of coal combustion by-product (CCBP) or coal ash and poses severe disposal problem. As each coal ash is distinct, this study presents the geochemistry of the coal ash, in particular fly ash, produced from the combustion of local coal from Kuching Sarawak, Malaysia. The geochemical composition of the ash showed a high amount of silica, alumina, iron oxides and alkalies which was found to be a convenient starting material for the hydrothermal synthesis of zeolites with the higher Na2O percentage being a positive factor for its alkaline activation; while the mineral phases are mainly quartz, mullite, calcium oxide, silica, and iron oxide hydrate. The geochemical changes upon alkali activation that can be predicted in a similar type of ash have been described in this paper. The result shows that this particular ash has a good potential for a high value industrial product like zeolites upon alkali activation.

Keywords: Coal ash, chemical composition, mineralogical composition, alkali activation, SEM.

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106 Mixed Convection in a 2D-channel with a Co- Flowing Fluid Injection: Influence of the Jet Position

Authors: Ameni Mokni, Hatem Mhiri, Georges Le Palec, Philippe Bournot

Abstract:

Numerical study of a plane jet occurring in a vertical heated channel is carried out. The aim is to explore the influence of the forced flow, issued from a flat nozzle located in the entry section of a channel, on the up-going fluid along the channel walls. The Reynolds number based on the nozzle width and the jet velocity ranges between 3 103 and 2.104; whereas, the Grashof number based on the channel length and the wall temperature difference is 2.57 1010. Computations are established for a symmetrically heated channel and various nozzle positions. The system of governing equations is solved with a finite volumes method. The obtained results show that the jet-wall interactions activate the heat transfer, the position variation modifies the heat transfer especially for low Reynolds numbers: the heat transfer is enhanced for the adjacent wall; however it is decreased for the opposite one. The numerical velocity and temperature fields are post-processed to compute the quantities of engineering interest such as the induced mass flow rate, and the Nusselt number along the plates.

Keywords: Channel, Heat flux, Jet, Mixed convection.

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105 Effect of Calcium Chloride on Rheological Properties and Structure of Inulin - Whey Protein Gels

Authors: Pawel Glibowski, Agnieszka Glibowska

Abstract:

The rheological properties, structure and potential synergistic interactions of whey proteins (1-6%) and inulin (20%) in mixed gels in the presence of CaCl2 was the aim of this study. Whey proteins have a strong influence on inulin gel formation. At low concentrations (2%) whey proteins did not impair in inulin gel formation. At higher concentration (4%) whey proteins impaired inulin gelation and inulin impaired the formation of a Ca2+-induced whey protein network. The presence of whey proteins at a level allowing for protein gel network formation (6%) significantly increased the rheological parameters values of the gels. SEM micrographs showed that whey protein structure was coated by inulin moieties which could make the mixed gels firmer. The protein surface hydrophobicity measurements did not exclude synergistic interactions between inulin and whey proteins, however. The use of an electrophoretic technique did not show any stable inulin-whey protein complexes.

Keywords: gels, hydrophobicity, inulin, SEM, whey proteins.

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104 Potential Role of Halophytic Macrophytes in Saline Effluent Treatment

Authors: R. Hegedűs, É. Kerepeczki, D. Gál, F. Pekár, M. Oncsik Bíróné, Gy. Lakatos

Abstract:

The growth of the aquaculture industry has been associated with negative environmental impacts through the discharge of raw effluents into the adjacent receiving water bodies. Macrophytes from natural saline lakes, which have adaptability to the high salinity, can be suitable for saline effluent treatment. Eight emergent species from natural saline area were planted in an experimental gravel bed hydroponic mesocosm (GBH) which was treated with effluent water from an intensive fish farm using geothermal water. In order to examine the applicability of the halophytes in treatment processes, we tested the relative efficacy of total nitrogen (TN), total phosphorus (TP), potassium (K), sodium (Na), magnesium (Mg) and calcium (Ca) removal for the saline wastewater treatment. Four of the eight species, which were Phragmites australis, Typha angustifolia, Glyceria maxima, Scirpus lacustris spp. tabernaemontani could survive and contribute the experimental treatment.

Keywords: Gravel bed hydroponic system, halophytes, intensive fish farm, salt removal

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103 Physicochemical Parameters of Tap Water in Dhahran, Saudi Arabia: An Empirical Assessment

Authors: Ahmed A. Hassan, Bassam Tawabini

Abstract:

In this study, the physicochemical parameters of Dhahran tap water were assessed to determine its suitability for drinking purposes. A total of 45 water samples were collected from different locations. The results indicate temperature ranges of 19.76 to 22.86 °C, pH (6.5 to 8.23), dissolved oxygen (4.21 to 8.32 mg/L), conductivity (232 to 2586 uS/cm), turbidity (0.17 to 0.37 Nephelometric Turbidity unit (NTU)), total dissolved solids (93 to 1671 mg/L), total alkalinity (4.11 to 24.04 mg/L), calcium (0.02 to 164 mg/L), magnesium (0 .6 to 77.9 mg/L), chloride (32.7 to 568.7 mg/L), nitrate (0.02 to 3 mg/L), fluoride (0.001 to 0.591 mg/L), sodium (18.4 to 232 mg/L), potassium (0.5 to 26.4 mg/L), and sulphate (2.39 to 258 mg/L). The results were compared with the drinking water standards recommended by the World Health Organization (WHO) and the United States Environmental Protection Agency (USEPA). The study determined that though the levels of most of the physicochemical parameters comply with the standards, however, slight deviations exist. This is evident in the values of the physical parameters (conductivity and total dissolved solids), and the chemical parameters (sulphate, chloride, and sodium) recorded at a few sample sites.

Keywords: Physicochemical parameters, tap water, water quality, Dhahran.

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102 Thermal Resistance Analysis of Flexible Composites Based on Al2O3 Aerogels

Authors: Jianzheng Wei, Duo Zhen, Zhihan Yang, Huifeng Tan

Abstract:

The deployable descent technology is a lightweight entry method using an inflatable heat shield. The heatshield consists of a pressurized core which is covered by different layers of thermal insulation and flexible ablative materials in order to protect against the thermal loads. In this paper, both aluminum and silicon-aluminum aerogels were prepared by freeze-drying method. The latter material has bigger specific surface area and nano-scale pores. Mullite fibers are used as the reinforcing fibers to prepare the aerogel matrix to improve composite flexibility. The flexible composite materials were performed as an insulation layer to an underlying aramid fabric by a thermal shock test at a heat flux density of 120 kW/m2 and uniaxial tensile test. These results show that the aramid fabric with untreated mullite fibers as the thermal protective layer is completely carbonized at the heat of about 60 s. The aramid fabric as a thermal resistance layer of the composite material still has good mechanical properties at the same heat condition.

Keywords: Aerogel, aramid fabric, flexibility, thermal resistance.

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101 Effect of Smoke Drying Techniques on the Proximate and Mineral Composition of Macrobrachium vollenhovenii (African River Prawn)

Authors: D. E. Omomo, R. M. Sunday, I. Kareem

Abstract:

This study was carried out to evaluate the nutritional composition of the African River Prawn (Macrobrachium vollenhovenii) in relation to Chokor (traditional) and Altona (improved traditional) drying techniques used in the preservation and processing of prawns by carrying out proximate composition analysis. The value obtained for the proximate analysis of Chokor and Altona smoke dried prawns were; Moisture (14.90% and 15.15%), Dry matter (85.10% and 84.85%), Protein (55.80% and 58.87%), Crude fat (1.95% and 1.98%), Crude fibre (21.40% and 13.11%), Carbohydrate (0.54% and 0.54%) and Ash (19.76% and 15.86%) respectively. The proximate mineral composition of Chokor and Altona smoke dried prawns were; Calcium (5.66% and 4.20%) and Phosphorus (9. 22% and 6.34%) respectively. Result shows there was no loss of nutritional value with respect to Chokor and Altona drying techniques used in the processing of prawns.

Keywords: Altona, Chokor, Macrobrachium vollenhovenii, Proximate composition, Smoke drying.

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100 Studies on the Blended Concrete Prepared with Tannery Effluent

Authors: K. Nirmalkumar

Abstract:

There is a acute water problem especially in the dry season in and around Perundurai (Erode district, Tamil Nadu, India) where there are more number of tannery units. Hence an attempt was made to use the waste water from tannery industry for construction purpose. The mechanical properties such as compressive strength, tensile strength, flexural strength etc were studied by casting various concrete specimens in form of cube, cylinders and beams etc and were found to be satisfactory. Hence some special properties such as chloride attack, sulphate attack and chemical attack are considered and comparatively studied with the conventional potable water. In this experimental study the results of specimens prepared by using treated and untreated tannery effluent were compared with the concrete specimens prepared by using potable water. It was observed that the concrete had some reduction in strength while subjected to chloride attack, sulphate attack and chemical attack. So admixtures were selected and optimized in suitable proportion to counter act the adverse effects and the results were found to be satisfactory.

Keywords: Calcium nitrite, concrete, fly ash.

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99 Effect of pH and Ionic Exchange on the Reactivity of Bioglass/Chitosan Composites Used as a Bone Graft Substitute

Authors: Samira Jebahi, Hassane Oudadesse, Eric Wers, Jiheun Elleuch, Hafedh Elfekih, Hassib Keskes, Xuan Vuong Bui, Abdelfatteh Elfeki

Abstract:

Chitosan (CH) material reinforced by bioactive glass (46S6) was fabricated. 46S6 containing 17% wt% CH was studied in vitro and in vivo. Physicochemical techniques, such as Fourier transform infrared spectroscopy (FT-IR), coupled plasma optical emission spectrometry (ICP-OES) analysis were used. The behavior of 46S6CH17 was studied by measuring the in situ pH in a SBF solution. The 46S6CH17 was implanted in the rat femoral condyl. In vitro 46S6CH17 gave an FTIR - spectrum in which three absorption bands with the maxima at 565, 603 and 1039cm-1 after 3 days of soaking in physiological solution. They are assigned to stretching vibrations of PO4^3- group in phosphate crystalline. Moreover, the pH measurement was decreased in the SBF solution. The stability of the calcium phosphate precipitation depended on the pH value. In vivo, a rise in the Ca and phosphate P ions concentrations in the implanted microenvironment was determined.

Keywords: Bioglass, Chitosan, pH measurement, Hydroxyapatite Carbonateted Layer.

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98 Synthesis of Aragonite Superstructure from Steelmaking Slag via Indirect CO2 Mineral Sequestration

Authors: Weijun Bao, Huiquan Li

Abstract:

Using steelmaking slag as a raw material, aragonite superstructure product had been synthesized via an indirect CO2 mineral sequestration rout. It mainly involved two separate steps, in which the element of calcium is first selectively leached from steelmaking slag by a novel leaching media consisting of organic solvent Tributyl phosphate (TBP), acetic acid, and ultra-purity water, followed by enhanced carbonation in a separate step for aragonite superstructure production as well as efficiency recovery of leaching media. Based on the different leaching medium employed in the steelmaking slag leaching process, two typical products were collected from the enhanced carbonation step. The products were characterized by X-ray powder diffraction (XRD) and scanning electron microscopy (SEM), respectively. It reveals that the needle-like aragonite crystals self-organized into aragonite superstructure particles including aragonite microspheres as well as dumbbell-like spherical particles, can be obtained from the steelmaking slag with the purity over 99%.

Keywords: Aragonite superstructure, Steelmaking slag, Indirect CO2 mineral sequestration, Selective leaching, Enhanced carbonation.

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97 Some Biochemical Changes Followed Experimental Gastric Ulceration

Authors: A. H. El-Far, R. R. Gindi, H. A. Abd El-Maksoud, Mohamed Ragaa Ragab Hassanien

Abstract:

Gastric ulceration is a discontinuity in gastric mucosa, usually occurs due to imbalance between the gastric mucosal protective factors, that is called gastric mucosal barrier, and the aggressive factors, to which the mucosa is exposed. This study was carried out on sixty male Sprague-Dowely rats (12- 16 weeks old) allocated into two groups. The first control group and the second Gastric lesion group which induced by oral administration of a single daily dose of aspirin at a dose of 300 mg/kg body weight for 7 consecutive-days (6% aspirin solution will be prepared and each rat will be given 5 ml of that solution/kg body weight). Blood is collected 1, 2 and 3 weeks after induction of gastric ulceration. Significant increase in serum copper, nitric oxide, and prostaglandin E2 all over the period of experiment. Significant decrease in erythrocyte superoxide dismutase (t-SOD) activities, serum (calcium, phosphorus, glucose and insulin) levels. Non-significant changes in serum sodium and potassium levels are obtained.

Keywords: Aspirin, Gastric Ulcer, Prostaglandin E2, Superoxide dismutase

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96 Some Physico-Chemical and Nutritional Properties of `Musmula` Medlar (Mespilus germanica L.) Grown in Northeast Anatolia

Authors: Ismail Hakki Kalyoncu, Nilda Ersoy, Ayse Yalcin Elidemir, Inci Tolay

Abstract:

In this study, The physico-chemical and nutritional properties of `Musmula` Medlar (Mespilus germanica L.) fruit and seed grown in Northeast Anatolia was investigated. In the fruit, length, width, thickness, weight, total soluble solids, colour (1), colour (2) [L, a, b values], protein, crude ash, crude fiber, crude oil, texture and pH were determinated as 4.34 cm, 4.22 cm, 3.67 cm, 38.36 g, 23.97 %, S60O60Y41,, [53.85, 17.15, 33.75], 1.06 %, 0.79 %, 4.24 %, 0.005 %, 1.21 kg/cm2 and 4.26 respectively. Also, pulp ratio, seed ratio and pulp/seed ratio were found to be 92.88 %, 7.11 % and 14.07 %, respectively. In addition, the mineral composition of medlar fruit in Northeast Anatolia was studied. In the fruit, 23 minerals were analyzed and 19 minerals were present at detectable levels. The medlar fruit was richest in potassium (6962 ppm), calcium (1186.378 ppm), magnesium (1070.08 ppm) and phosphor (763.425 ppm).

Keywords: Fruits, Mespilus germanica L., mineral compounds, physico-chemical properties.

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95 Identification of Binding Proteins That Interact with BVDV E2 Protein in Bovine Trophoblast Cell

Authors: Yan Ren, Fei Guo, Jun Qiao, Shengwei Hu, Hui Zhang, Yuanzhi Wang, Pengyan Wang, Jinliang Sheng, Xinli Gu, Xiaojun Liu, Chuangfu Chen

Abstract:

Bovine viral diarrhea virus (BVDV) can cause lifelong persistent infection. One reason for the phenomena is attributed to BVDV infection to placenta tissue. However the mechanisms that BVDV invades into placenta tissue remain unclear. To clarify the molecular mechanisms, we investigated the possible means that BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid system was used to identify proteins extracted from TPC, which interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast expression vector and TPC cDNA library were constructed. Through two rounds of screening, three positive clones were identified. Sequencing analysis indicated that all the three positive clones encoded the same protein clathrin. Physical interaction between clathrin and BVDV E2 protein was further confirmed by coimmunoprecipitation experiments. This result suggested that the clathrin might play a critical role in the process of BVDV entry into placenta tissue and might be a novel antiviral target for preventing BVDV infection.

Keywords: Bovine viral diarrhea virus, clathrin, glycoprotein E2, yeast two-hybrid system.

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94 Effect of Plasticizer Additives on the Mechanical Properties of Cement Composite – A Molecular Dynamics Analysis

Authors: R. Mohan, V. Jadhav, A. Ahmed, J. Rivas, A. Kelkar

Abstract:

Cementitious materials are an excellent example of a composite material with complex hierarchical features and random features that range from nanometer (nm) to millimeter (mm) scale. Multi-scale modeling of complex material systems requires starting from fundamental building blocks to capture the scale relevant features through associated computational models. In this paper, molecular dynamics (MD) modeling is employed to predict the effect of plasticizer additive on the mechanical properties of key hydrated cement constituent calcium-silicate-hydrate (CSH) at the molecular, nanometer scale level. Due to complexity, still unknown molecular configuration of CSH, a representative configuration widely accepted in the field of mineral Jennite is employed. The effectiveness of the Molecular Dynamics modeling to understand the predictive influence of material chemistry changes based on molecular / nanoscale models is demonstrated.

Keywords: Cement composite, Mechanical Properties, Molecular Dynamics, Plasticizer additives.

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93 Simulation of Enhanced Biomass Gasification for Hydrogen Production using iCON

Authors: Mohd K. Yunus, Murni M. Ahmad, Abrar Inayat, Suzana Yusup

Abstract:

Due to the environmental and price issues of current energy crisis, scientists and technologists around the globe are intensively searching for new environmentally less-impact form of clean energy that will reduce the high dependency on fossil fuel. Particularly hydrogen can be produced from biomass via thermochemical processes including pyrolysis and gasification due to the economic advantage and can be further enhanced through in-situ carbon dioxide removal using calcium oxide. This work focuses on the synthesis and development of the flowsheet for the enhanced biomass gasification process in PETRONAS-s iCON process simulation software. This hydrogen prediction model is conducted at operating temperature between 600 to 1000oC at atmospheric pressure. Effects of temperature, steam-to-biomass ratio and adsorbent-to-biomass ratio were studied and 0.85 mol fraction of hydrogen is predicted in the product gas. Comparisons of the results are also made with experimental data from literature. The preliminary economic potential of developed system is RM 12.57 x 106 which equivalent to USD 3.77 x 106 annually shows economic viability of this process.

Keywords: Biomass, Gasification, Hydrogen, iCON.

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92 Production of H5N1 Hemagglutinin inTrichoplusia ni Larvae by a Novel Bi-cistronic Baculovirus Expression Vector

Authors: Tzyy Rong Jinn, Nguyen Tiep Khac, Tzong Yuan Wu

Abstract:

Highly pathogenic avian influenza (HPAI) H5N1 viruses have created demand for a cost-effective vaccine to prevent a pandemic of the disease. Here, we report that Trichoplusia ni (T. ni) larvae can act as a cost-effective bioreactor to produce recombinant HA5 (rH5HA) proteins as an potential effective vaccine for chickens. To facilitate the recombinant virus identification, virus titer determination and access the infected larvae, we employed the internal ribosome entry site (IRES) derived from Perina nuda virus (PnV, belongs to insect picorna like Iflavirus genus) to construct a bi-cistronic baculovirus expression vector that can express the rH5HA protein and enhanced green fluorescent protein (EGFP) simultaneously. Western blot analysis revealed that the 70 kDa rH5HA protein and partially cleaved products (40 kDa H5HA1) were generated in T. ni larvae infected with recombinant baculovirus carrying the H5HA gene. These data suggest that the baculovirus-larvae recombinant protein expression system could be a cost-effective platform for H5N1 vaccine production.

Keywords: Avian Influenza, baculovirus, hemagglutinin, Trichoplusia ni larvae

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91 Multilayer Soft Tissue Continuum Model: Towards Realistic Simulation of Facial Expressions

Authors: A. Hung, K. Mithraratne, M. Sagar, P. Hunter

Abstract:

A biophysically based multilayer continuum model of the facial soft tissue composite has been developed for simulating wrinkle formation. The deformed state of the soft tissue block was determined by solving large deformation mechanics equations using the Galerkin finite element method. The proposed soft tissue model is composed of four layers with distinct mechanical properties. These include stratum corneum, epidermal-dermal layer (living epidermis and dermis), subcutaneous tissue and the underlying muscle. All the layers were treated as non-linear, isotropic Mooney Rivlin materials. Contraction of muscle fibres was approximated using a steady-state relationship between the fibre extension ratio, intracellular calcium concentration and active stress in the fibre direction. Several variations of the model parameters (stiffness and thickness of epidermal-dermal layer, thickness of subcutaneous tissue layer) have been considered.

Keywords: Bio-physically based, soft tissue mechanics, facialtissue composite, wrinkling.

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90 Aerodynamic Design of Three-Dimensional Bellmouth for Low-Speed Open-Circuit Wind Tunnel

Authors: Harshavardhan Reddy, Balaji Subramanian

Abstract:

A systematic parametric study to find the optimum Bellmouth profile by relating geometric and performance parameters to satisfy a set of specifications is reported. A careful aerodynamic design of Bellmouth intake is critical to properly direct the flow with minimal losses and maximal flow uniformity into the honeycomb located inside the settling chamber of an indraft wind tunnel, thus improving the efficiency of the entire unit. Design charts for elliptically profiled Bellmouth's with two different contraction ratios (9 and 18) and three different test section speeds (25 m/s, 50 m/s, and 75 m/s) were presented. A significant performance improvement - especially in the coefficient of discharge and in the flow angularity and boundary layer thickness at the honeycomb inlet - was observed when an entry corner radius (r/D = 0.08) was added to the Bellmouth profile. The nonuniformity at the honeycomb inlet drops by about three times (~1% to 0.3%) when moving from square to regular octagonal cross-section. An octagonal cross-sectioned Bellmouth intake with L/d = 0.55, D/d = 1.625, and r/D = 0.08 met all the four target performance specifications and is proposed as the best choice for a low-speed wind tunnel.

Keywords: Bellmouth intake, low-speed wind tunnel, coefficient of discharge, nonuniformity, flow angularity, boundary layer thickness, CFD, aerodynamics.

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89 Accessibility and Visibility through Space Syntax Analysis of the Linga Raj Temple in Odisha, India

Authors: S. Pramanik

Abstract:

Since the early ages, the Hindu temples have been interpreted through various Vedic philosophies. These temples are visited by pilgrims which demonstrate the rituals and religious belief of communities, reflecting a variety of actions and behaviors. Darsana a direct seeing, is a part of the pilgrimage activity. During the process of Darsana, a devotee is prepared for entry in the temple to realize the cognizing Truth culminating in visualizing the idol of God, placed at the Garbhagriha (sanctum sanctorum). For this, the pilgrim must pass through a sequential arrangement of spaces. During the process of progress, the pilgrims visualize the spaces differently from various points of views. The viewpoints create a variety of spatial patterns in the minds of pilgrims coherent to the Hindu philosophies. The space organization and its order are perceived by various techniques of spatial analysis. A temple, as examples of Kalinga stylistic variations, has been chosen for the study. This paper intends to demonstrate some visual patterns generated during the process of Darsana (visibility) and its accessibility by Point Isovist Studies and Visibility Graph Analysis from the entrance (Simha Dwara) to The Sanctum sanctorum (Garbhagriha).

Keywords: Hindu Temple Architecture, Point Isovist, space syntax analysis, visibility graph analysis.

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88 CFD Simulation and Validation of Flow Pattern Transition Boundaries during Moderately Viscous Oil-Water Two-Phase Flow through Horizontal Pipeline

Authors: Anand B. Desamala, Anjali Dasari, Vinayak Vijayan, Bharath K. Goshika, Ashok K. Dasmahapatra, Tapas K. Mandal

Abstract:

In the present study, computational fluid dynamics (CFD) simulation has been executed to investigate the transition boundaries of different flow patterns for moderately viscous oil-water (viscosity ratio 107, density ratio 0.89 and interfacial tension of 0.032 N/m.) two-phase flow through a horizontal pipeline with internal diameter and length of 0.025 m and 7.16 m respectively. Volume of Fluid (VOF) approach including effect of surface tension has been employed to predict the flow pattern. Geometry and meshing of the present problem has been drawn using GAMBIT and ANSYS FLUENT has been used for simulation. A total of 47037 quadrilateral elements are chosen for the geometry of horizontal pipeline. The computation has been performed by assuming unsteady flow, immiscible liquid pair, constant liquid properties, co-axial flow and a T-junction as entry section. The simulation correctly predicts the transition boundaries of wavy stratified to stratified mixed flow. Other transition boundaries are yet to be simulated. Simulated data has been validated with our own experimental results.

Keywords: CFD simulation, flow pattern transition, moderately viscous oil-water flow, prediction of flow transition boundary, VOF technique.

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87 Extraction of Craniofacial Landmarks for Preoperative to Intraoperative Registration

Authors: M. Gooroochurn, D. Kerr, K. Bouazza-Marouf, M. Vloeberghs

Abstract:

This paper presents the automated methods employed for extracting craniofacial landmarks in white light images as part of a registration framework designed to support three neurosurgical procedures. The intraoperative space is characterised by white light stereo imaging while the preoperative plan is performed on CT scans. The registration aims at aligning these two modalities to provide a calibrated environment to enable image-guided solutions. The neurosurgical procedures can then be carried out by mapping the entry and target points from CT space onto the patient-s space. The registration basis adopted consists of natural landmarks (eye corner and ear tragus). A 5mm accuracy is deemed sufficient for these three procedures and the validity of the selected registration basis in achieving this accuracy has been assessed by simulation studies. The registration protocol is briefly described, followed by a presentation of the automated techniques developed for the extraction of the craniofacial features and results obtained from tests on the AR and FERET databases. Since the three targeted neurosurgical procedures are routinely used for head injury management, the effect of bruised/swollen faces on the automated algorithms is assessed. A user-interactive method is proposed to deal with such unpredictable circumstances.

Keywords: Face Processing, Craniofacial Feature Extraction, Preoperative to Intraoperative Registration, Registration Basis.

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86 Realignment of f-actin Cytoskeleton in Osteocytes after Mechanical Loading

Authors: R. S. A. Nesbitt, J. Macione, E. Babollah, B. Adu-baffour, S. P. Kotha

Abstract:

F-actin fibrils are the cytoskeleton of osteocytes. They react in a dynamic manner to mechanical loading, and strength and reposition their efforts to reinforce the cells structure. We hypothesize that f-actin is temporarly disrupted after loading and repolymerizes in a new orientation to oppose the applied load. In vitro studies are conducted to determine f-actin disruption after varying mechanical stimulus parameters that are known to affect bone formation. Results indicate that the f-actin cytoskeleton is disrupted in vitro as a function of applied mechanical stimulus parameters and that the f-actin bundles reassemble after loading induced disruption within 3 minutes after cessation of loading. The disruption of the factin cytoskeleton depends on the magnitude of stretch, the numbers of loading cycles, frequency, the insertion of rest between loading cycles and extracellular calcium. In vivo studies also demonstrate disruption of the f-actin cytoskeleton in cells embedded in the bone matrix immediately after mechanical loading. These studies suggest that adaptation of the f-actin fiber bundles of the cytoskeleton in response to applied loads occurs by disruption and subsequent repolymerization.

Keywords: Mechanical loading of osteocytes, f-actin cytoskeleton, disruption, re-polymerization.

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85 Utilization of Mustard Leaves (Brassica juncea) Powder for the Development of Cereal Based Extruded Snacks

Authors: Maya S. Rathod, Bahadur Singh Hathan

Abstract:

Mustard leaves are rich in folates, vitamin A, K and B-complex. Mustard greens are low in calories and fats and rich in dietary fiber. They are rich in potassium, manganese, iron, copper, calcium, magnesium and low in sodium. It is very rich in antioxidants and Phytonutrients. For the optimization of process variables (moisture content and mustard leave powder), the experiments were conducted according to central composite Face Centered Composite design of RSM. The mustard leaves powder was replaced with composite flour (a combination of rice, chickpea and corn in the ratio of 70:15:15). The extrudate was extruded in a twin screw extruder at a barrel temperature of 120°C. The independent variables were mustard leaves powder (2-10 %) and moisture content (12-20 %). Responses analyzed were bulk density, water solubility index, water absorption index, lateral expansion, antioxidant activity, total phenolic content, and overall acceptability. The optimum conditions obtained were 7.19 g mustard leaves powder in 100g premix having 16.8% moisture content (w.b).

Keywords: Extrusion, mustard leaves powder, optimization, response surface methodology.

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84 Modeling the Country Selection Decision in Retail Internationalization

Authors: A. Hortacsu, A. Tektas

Abstract:

This paper aims to develop a model that assists the international retailer in selecting the country that maximizes the degree of fit between the retailer-s goals and the country characteristics in his initial internationalization move. A two-stage multi criteria decision model is designed integrating the Analytic Hierarchy Process (AHP) and Goal Programming. Ethical, cultural, geographic and economic proximity are identified as the relevant constructs of the internationalization decision. The constructs are further structured into sub-factors within analytic hierarchy. The model helps the retailer to integrate, rank and weigh a number of hard and soft factors and prioritize the countries accordingly. The model has been implemented on a Turkish luxury goods retailer who was planning to internationalize. Actual entry of the specific retailer in the selected country is a support for the model. Implementation on a single retailer limits the generalizability of the results; however, the emphasis of the paper is on construct identification and model development. The paper enriches the existing literature by proposing a hybrid multi objective decision model which introduces new soft dimensions i.e. perceived distance, ethical proximity, humane orientation to the decision process and facilitates effective decision making.

Keywords: Analytic hierarchy process, culture, ethics, goal programming, retail foreign market selection.

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