Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 29994
Identification of Binding Proteins That Interact with BVDV E2 Protein in Bovine Trophoblast Cell

Authors: Yan Ren, Fei Guo, Jun Qiao, Shengwei Hu, Hui Zhang, Yuanzhi Wang, Pengyan Wang, Jinliang Sheng, Xinli Gu, Xiaojun Liu, Chuangfu Chen

Abstract:

Bovine viral diarrhea virus (BVDV) can cause lifelong persistent infection. One reason for the phenomena is attributed to BVDV infection to placenta tissue. However the mechanisms that BVDV invades into placenta tissue remain unclear. To clarify the molecular mechanisms, we investigated the possible means that BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid system was used to identify proteins extracted from TPC, which interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast expression vector and TPC cDNA library were constructed. Through two rounds of screening, three positive clones were identified. Sequencing analysis indicated that all the three positive clones encoded the same protein clathrin. Physical interaction between clathrin and BVDV E2 protein was further confirmed by coimmunoprecipitation experiments. This result suggested that the clathrin might play a critical role in the process of BVDV entry into placenta tissue and might be a novel antiviral target for preventing BVDV infection.

Keywords: Bovine viral diarrhea virus, clathrin, glycoprotein E2, yeast two-hybrid system.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1333188

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF

References:


[1] Xiang, X., Duan, G., Li, Z. The Advance of Molecular Biology Research of BVDV. Acta Ecologiae Animalis Domastici, 2004, 25(4): 202-204.
[2] Vidricaire, G., Tardif, M. R., Tremblay, M. J. The low viral production in trophoblastic cells is due to a high endocytif internalization of the human immunodeficiency virus type 1 and can be overcome by the pro-inflammatory cytokines tumor necrosis factor-╬▒ and interleukin-1. J biol chemistry, 2003, 278 (18): 15832-15841.
[3] Shearer, W. T., Reuben, J., Lee, B. N., Popek, E. J., Lewis, D. E., Hammill, H. H., Hanson, I. C., Kline, M. W., Langston, C. Role of placental cytokines and inflammation in vertical transmission of HIV infection. Acta Paediatr Supp, 1997, 421:33-8.
[4] Moussa, M., Mognetti, B., Dubanchet, S., Menu, E., Roques, P., Gras, G., Dormont, D., Barre-Sinoussi, F.,Chaouat, G. Vertical transmission of HIV: parameters which might affect infection of placental trophoblasts by HIV-1: a review. Biomed group on the study of in utero transmission of HIV-1. Am J Reprod Immunol, 1999, 41(5):312-9.
[5] Parry, S., Holder, J., Strauss, J. F., Mechanisms of trophoblast-virus interaction. J Reprod Immunol, 1997, 37(1):25-34.
[6] Gan, X., Hou, R., Zhang, M., Zhang, H. Role of Trophocytes in Mammalian Embryo Implantation. J Sichuan Zoology, 2005, 24(4): 660-664.
[7] Ren, Y., Chen, C., Qiao, J., Wang, Y., Guo, Q., Zhang, H., Wang, P. Culture of trophoblast giant cells from bovine placentomes. Chin J Cell Biol, 2009, 29(7): 541-549.
[8] Wang, Z., Ding, M. Research advances on Bovine viral diarrhea virus. Virological Sinica, 1997, 12(2): 112-118.
[9] Yu, M., Gould, A. R., Morrissy, C. J. High level expression of the envelope glycoprotein(gp53) of bovine viral diarrhea virus (Singer) and its potential use as diagnostic reagent. Virus Res, 1994, 34: 178-186.
[10] Donis, R. O., Corapi, W. V., Dubovi, E. J. Bovine viral diarrhea virus proteins and their antigenic analysis. Arch Virol, 1991, Suppl 3:29-40.
[11] Jiang, X., Yang, J., Ren, K. The yeast two-hybrid system. Chin J Cell Biol, 2000, 22(4): 176-181.
[12] Zahoor, M. A., Yamane, D., Mohamed, Y. M., Suda, Y., Kobayashi, K., Kato, K., Tohy,a Y., Akashi, H. Bovine viral diarrhea virus non-structural protein 5A interacts with NIK- and IKKbeta-binding protein. J Gen Virol, 2010, 91(8):1939-48.
[13] Grummer, B., Beer, M., Liebler-Tenorio, E., Greiser-Wilke, I. Localization of viral proteins in cells infected with bovine viral diarrhoea virus. J Gen Virol, 2001, 82(11):2597-605.
[14] Chimeno Zoth, S., Leunda, M. R., Ode├│n, A., Taboga, O. Recombinant E2 glycoprotein of bovine viral diarrhea virus induces a solid humoral neutralizing immune response but fails to confer total protection in cattle. J Virol Methods, 2006, 138(1-2):99-108.
[15] Chimeno Zoth, S., Taboga, O. Multiple recombinant ELISA for the detection of bovine viral diarrhoea virus antibodies in cattle sera. J Virol Methods, 2006,138(1-2):99-108.
[16] Botton, S. A., da-Silva, A. M., Brum, M. C., Weiblen, R., Flores, E. F. Antigenic characterization of Brazilian bovine viral diarrhea virus isolates by monoclonal antibodies and cross-neutralization. Braz J Med Biol Res, 1998, 31(11):1429-38.
[17] Li, M. F., Shi, Y. L. The mechanism of clathrin-mediatedsynaptic vesicle endocytosis. Chin J Neurosci, 2002, 18(3):643-647.
[18] Lecot, S., Belouzard, S., Dubuisson, J., Rouillé, Y. Bovine Viral Diarrhea Virus Entry Is Dependent on Clathrin-Mediated Endocytosis. J Virol, 2005, 79(16):10826-9.
[19] Chu, J. J. H., and M. L. Ng. Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway. J. Virol, 2004, 78:10543-10555.
[20] Grummer, B., Grotha, S. and Greiser-Wilke, I. Bovine viral diarrhea virus is internalized by clathrin-dependent receptor-mediated endocytosis. J. Vet. Med. B Infect. Dis. Vet. Public Health, 2004, 51:427-432.
[21] Gruenberg, J. Viruses and endosome membrane dynamics. Curr Opin Cell Biol, 2009, 21(4):582-8.
[22] Liang, M., Morizono, K., Pariente, N., Kamata, M., Lee, B., Chen, I. S. Targeted transduction via CD4 by a lentiviral vector uses a clathrin-mediated entry pathway. J Virol, 2009, 83(24):13026-31.
[23] Sun, X., Yau, V. K., Briggs, B. J., Whittaker, G. R. Role of clathrin-mediated endocytosis during vesicular stomatitis virus entry into host cells. Virology, 2005, 338(1):53-60.
[24] Jin, M., Park, J., Lee, S., Park, B., Shin, J., Song, K. J., Ahn, T. I., Hwang, S. Y., Ahn, B. Y., Ahn, K. Hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis. Virology, 2002, 294(1):60-9.
[25] Querbes, W., Benmerah, A., Tosoni, D., Di Fiore, P. P., Atwood, W. J. A JC virus-induced signal is required for infection of glial cells by a clathrin- and eps15-dependent pathway. J Virol, 2004,78(1):250-6.
[26] Suikkanen, S., Sääjärvi, K., Hirsimäki, J., Välilehto, O., Reunanen, H.,Vihinen-Ranta, M., Vuento, M. Role of recycling endosomes and lysosomes in dynein-dependent entry of canine parvovirus. J Virol, 2002, 76(9):4401-11.
[27] Ruiz-Sáenz, J., Goez, Y., Tabares, W., López-Herrera, A. Cellular receptors for foot and mouth disease virus. Intervirology, 2009, 52(4):201-12.
[28] Schmid, S. L. Clathrin coated vesicle formation and protein sorting an integrated process. Annu Rev Biochem, 1997, 66:511-548.
[29] Pelkmans, L., Helenius, A. Endocytosis via caveolae. Traffic, 2002, 3(5):311
[30] Brodsky, F. M. Clathrin light chains: array of protein M otifs that regulate coated-vesicle dynam ics. Trends Biology Sci, 1991, 16:208.
[31] Ihrke, G., Hubbard, A. L. Control of vesicle traffic in hepatocyte. Prog Liver Bis, 1995, 13:63.
[32] Sle pnev, V. I., De Camilli, P. Accessory factors in clathrin dependent synaptic vesicle endocytosis . Nat Rev Neurosci, 2000, 1:161-172.