Search results for: ecological species guild

Authors: Kacharava Tamar, Korakhashvili Avtandil, Epitashvili Tinatin

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The article deals with the database, which was created by the authors, related to biodiversity of some families of useful plants (medicinal, aromatic, spices, dye and poisonous) existing in Georgia considering important taxonomy. Our country is also rich with endemic genera. The results of monitoring of the phytogenetic resources to reveal perspective species and situation of endemic species and resources are also discussed in this paper. To get some new medicinal and preventive treatments using plant raw material in the phytomedicine, phytocosmetics and phytoculinary, the unique phytogenetic resources should be protected because the application of useful plants is becoming irreversible. This can be observed along with intensification and sustainable use of ethnobotanical traditions and promotion of phytoproduction based on the international requirements on biodiversity (Convention on Biological Diversity - CBD). Though Georgian phytopharmacy has the centuries-old traditions, today it is becoming the main concern.

Keywords: Aromatic, medicinal, poisonous, spicy, dye plants, endemic biodiversity, endemic, ELISA, GIS.

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Authors: Mohamed F. Awad, M. Kraume

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The objective of this study is to evaluate the occurrence of fungi in aerobic and anoxic activated sludge from membrane bioreactors (MBRs). Thirty-six samples of both aerobic and anoxic activated sludge were taken from 2 MBR treating domestic wastewater. Over a period of eight months 2 samples from each plant were taken per month. The samples were prepared for count and definition of fungi. The obtained data show that, sixty species belonging to 27 genera were collected from activated sludge samples under aerobic and anoxic conditions. Regarding to the fungi definition, under aerobic condition the Geotrichum was found at (8.8%) followed by Penicillium (75.0%), Yeasts (65.7%) and Trichoderma (55.5%), while Yeasts (77.1%) Geotrichum candidumand Penicillium (61.1%) species were the most prevalent in anoxic activated sludge. The results indicate that activated sludge is habitat for growth and sporulation of different groups of fungi, both saprophytic and pathogenic.

Keywords: Aerobic conditions, Anoxic conditions, Activated sludge, Membrane bioreactor, Fungi.

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Authors: Zainab M. Almutairi, Amjad Alharbi

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The use of engineered nanomaterials has increased as a result of their positive impact on many sectors of the economy, including agriculture. Silver nanoparticles (AgNPs) are now used to enhance seed germination, plant growth, and photosynthetic quantum efficiency and as antimicrobial agents to control plant diseases. In this study, we examined the effect of AgNP dosage on the seed germination of three plant species: corn (Zea mays L.), watermelon (Citrullus lanatus [Thunb.] Matsum. & Nakai) and zucchini (Cucurbita pepo L.). This experiment was designed to study the effect of AgNPs on germination percentage, germination rate, mean germination time, root length and fresh and dry weight of seedlings for the three species. Seven concentrations (0.05, 0.1, 0.5, 1, 1.5, 2 and 2.5 mg/ml) of AgNPs were examined at the seed germination stage. The three species had different dose responses to AgNPs in terms of germination parameters and the measured growth characteristics. The germination rates of the three plants were enhanced in response to AgNPs. Significant enhancement of the germination percentage values was observed after treatment of the watermelon and zucchini plants with AgNPs in comparison with untreated seeds. AgNPs showed a toxic effect on corn root elongation, whereas watermelon and zucchini seedling growth were positively affected by certain concentrations of AgNPs. This study showed that exposure to AgNPs caused both positive and negative effects on plant growth and germination.

Keywords: Citrullus lanatus, Cucurbita pepo, seed germination, seedling growth, silver nanoparticles, Zea mays.

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Authors: Zainab M. Almutairi, Amjad Alharbi

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The use of engineered nanomaterials has increased as a result of their positive impact on many sectors of the economy, including agriculture. Silver nanoparticles (AgNPs) are now used to enhance seed germination, plant growth, and photosynthetic quantum efficiency and as antimicrobial agents to control plant diseases. In this study, we examined the effect of AgNP dosage on the seed germination of three plant species: corn (Zea mays L.), watermelon (Citrullus lanatus [Thunb.] Matsum. & Nakai) and zucchini (Cucurbita pepo L.). This experiment was designed to study the effect of AgNPs on germination percentage, germination rate, mean germination time, root length and fresh and dry weight of seedlings for the three species. Seven concentrations (0.05, 0.1, 0.5, 1, 1.5, 2 and 2.5 mg/ml) of AgNPs were examined at the seed germination stage. The three species had different dose responses to AgNPs in terms of germination parameters and the measured growth characteristics. The germination rates of the three plants were enhanced in response to AgNPs. Significant enhancement of the germination percentage values was observed after treatment of the watermelon and zucchini plants with AgNPs in comparison with untreated seeds. AgNPs showed a toxic effect on corn root elongation, whereas watermelon and zucchini seedling growth were positively affected by certain concentrations of AgNPs. This study showed that exposure to AgNPs caused both positive and negative effects on plant growth and germination.

Keywords: Citrullus lanatus, Cucurbita pepo, seed germination, seedling growth, silver nanoparticles, Zea mays.

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Authors: S. Piratheepa, G. Rajendramani, T. Eswaramohan

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Current study was conducted for one year from June 2014 to May 2015, with an objective of identification of fish and shellfish diversity in the Thondamanaru lagoon ecosystem. In this study, 11 species were identified from Thondamanaru lagoon, Jaffna, Sri Lanka. There are four fishes, Chanos chanos, Hemirhamphus sp., Nematalosa sp. and Mugil cephalus and seven shell fishes, Penaeus indicus, Penaeus monodon, Penaeus latisulcatus, Penaeus semisulcatus, Metapenaeus monoceros, Portunus pelagicus and Scylla serrata. Species composition of Mugil cephalus, Penaeus indicus and Metapenaeus monoceros was high during rainy seasons. However, lagoon is being subjected to adverse environmental conditions that threaten its fish and shellfish biodiversity due to lack of saline water availability and changes in rainfall pattern.

Keywords: Diversity, shell fish, shrimp, Thondamanaru lagoon.

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Authors: O. Zennouhi, M. El Mderssa, J. Ibijbijen, E. Bouiamrine, L. Nassiri

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Rangelands and silvopastoral systems of the middle Atlas are under a heavy pressure, which led to pasture degradation, invasion by non-palatable and toxic species and edaphic aridification due to the regression of the global vegetation cover. In this situation, the introduction of multipurpose leguminous shrubs, such as Bituminaria bituminosa (L.) Stirton, commonly known as bituminous clover, could be a promising socio-ecological alternative for the rehabilitation of these degraded areas. The application of biofertilizers like plant growth promoting rhizobacteria especially phosphate solubilizing bacteria (PSB) can ensure a successful installation of this plant in the selected degraded areas. The main objective of the present work is to produce well-inoculated seedlings using the best efficient PSB strains in the greenhouse to increase their ability to resist to environmental constraints once transplanted to the field in the central Middle Atlas.

Keywords: Biofertilizers, Bituminaria bituminosa, phosphate solubilizing bacteria, rehabilitation.

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Authors: Shamim Ahmed Koichi Nishigaki

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Genome profiling (GP), a genotype based technology, which exploits random PCR and temperature gradient gel electrophoresis, has been successful in identification/classification of organisms. In this technology, spiddos (Species identification dots) and PaSS (Pattern similarity score) were employed for measuring the closeness (or distance) between genomes. Based on the closeness (PaSS), we can buildup phylogenetic trees of the organisms. We noticed that the topology of the tree is rather robust against the experimental fluctuation conveyed by spiddos. This fact was confirmed quantitatively in this study by computer-simulation, providing the limit of the reliability of this highly powerful methodology. As a result, we could demonstrate the effectiveness of the GP approach for identification/classification of organisms.

Keywords: Fluctuation, Genome profiling (GP), Pattern similarity score (PaSS), Robustness, Spiddos-shift.

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Authors: M. A. Gad, A. Z. Al-Herrawy

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Microsporidia comprises various pathogenic species can infect humans by means of water. Moreover, chlorine disinfection of drinking-water has limitations against this protozoan pathogen. A total of 48 water samples were collected from two drinking water treatment plants having two different filtration systems (slow sand filter and rapid sand filter) during one year period. Samples were collected from inlet and outlet of each plant. Samples were separately filtrated through nitrocellulose membrane (142 mm, 0.45 µm), then eluted and centrifuged. The obtained pellet from each sample was subjected to DNA extraction, then, amplification using genus-specific primer for microsporidia. Each microsporidia-PCR positive sample was performed by two species specific primers for Enterocytozoon bieneusi and Encephalitozoon intestinalis. The results of the present study showed that the percentage of removal for microsporidia through different treatment processes reached its highest rate in the station using slow sand filters (100%), while the removal by rapid sand filter system was 81.8%. Statistically, the two different drinking water treatment plants (slow and rapid) had significant effect for removal of microsporidia. Molecular identification of microsporidia-PCR positive samples using two different primers for Enterocytozoon bieneusi and Encephalitozoon intestinalis showed the presence of the two pervious species in the inlet water of the two stations, while Encephalitozoon intestinalis was detected in the outlet water only. In conclusion, the appearance of virulent microsporidia in treated drinking water may cause potential health threat.

Keywords: Removal, efficacy, microsporidia, drinking water treatment plants, PCR.

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Authors: Kiomars Sefidi

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Dead wood and habitat tree such as fallen logs, snags, stumps and cracks and loos bark etc. are regarded as an important ecological component of forests on which many forest dwelling species depend on presence of them within forest ecosystems. Meanwhile its relation to management history in Caspian forest has gone unreported. The aim of research was to compare the amounts of dead wood and habitat trees in the forests with historically different intensities of management, including: forests with the long term implication of management (PS), the short term implication of management (NS) which were compared with semi virgin forest (GS). The number of 405 individual dead and habitat trees were recorded and measured at 109 sampling locations. ANOVA revealed volume of dead tree in the form and decay classes significantly differ within sites and dead volume in the semi virgin forest significantly higher than managed sites. Comparing the amount of dead and habitat tree in three sites showed that, dead tree volume related with management history and significantly differ in three study sites. Meanwhile, frequency of habitat trees was significantly different within sites. The highest amount of habitat trees including cavities, cracks and loose bark and fork split trees was recorded in virgin site and lowest recorded in the sites with the long term implication of management. It can be concluded that forest management cause reduction of the amount of dead and habitat tree specially in a large size, thus managing this forest according to ecological sustainable principles require a commitment to maintaining stand structure that allow, continued generation of dead trees in a full range of size.

Keywords: Cracks trees, forest biodiversity, fork split trees, nature conservation, sustainable management.

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Authors: J. Winkler, S. Chovancová

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The article deals with the relation between rainfall in selected months and subsequent weed infestation of spring barley. The field experiment was performed at Mendel University agricultural enterprise in Žabčice, Czech Republic. Weed infestation was measured in spring barley vegetation in years 2004 to 2012. Barley was grown in three tillage variants: conventional tillage technology (CT), minimization tillage technology (MT), and no tillage (NT). Precipitation was recorded in one-day intervals. Monthly precipitation was calculated from the measured values in the months of October through to April. The technique of canonical correspondence analysis was applied for further statistical processing. 41 different species of weeds were found in the course of the 9-year monitoring period. The results clearly show that precipitation affects the incidence of most weed species in the selected months, but acts differently in the monitored variants of tillage technologies.

Keywords: Weeds, precipitation, tillage, weed infestation forecast.

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Authors: Mahbobeh Hajirostamloo

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Ligula intestinalis is a three-host life-cycle Pseudophyllidean Cestode which in its plerocercoid stage infests a range of fresh water species. The objective of the present study was the worm occurrence within planctonic copepods, fishes and piscivorous birds and examine of parasite-hosts samples in the Lake of Sattarkhan Dam (near the city of Ahar, East Azerbaijan, Iran). Fish sample were collected with fyke and gill nets and the abdominal cavity was examined for the presence of ligula. Zooplanktons were captured using a planktonic net and occurrence of parasitic larval form in the body cavity was determined. Piscivorous birds were selected by telescope, they hunted and dissected for presence of parasite eggs in their gut. Results indicated that prevalence of infection was 16% for cyclopid copepoda and majority of infected cyclopid were female Cyclops. Investigation of 310 fishes specimens were indicated to infection of five species of cyprinid fishes. In addition, results indicated to manipulation of six species of migratory aquatic and semi aquatic birds by ligula. Obtained results are in agreement by previous studies. Its definite in this study that all of fishes in Sattarkhan Lake capable to infection, its important for health because they capture by native people and it is documented that ligula can be introduce as a zoonose. It's seemed that to prevent from disperses of parasite and restricted of infection, biological elimination can be effective and it's necessary to inform native people about sanitation.

Keywords: Ligula intestinalis, parasite-host, Sattarkhan Lake, Iran.

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Authors: Amr A. El Hanafy, Yasir Anwar, Saleh A. Mohamed, Saleh Mohamed Saleh Al-Garni, Jamal S. M. Sabir, Osama A. H. Abu Zinadah, Mohamed Morsi Ahmed

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In the vicinity of red sea about 15 fungi species were isolated from oil contaminated sites. On the basis of aptitude to degrade the crude oil and DCPIP assay, two fungal isolates were selected amongst 15 oil degrading strains. Analysis of ITS-1, ITS-2 and amplicon pyrosequencing studies of fungal diversity revealed that these strains belong to Penicillium and Aspergillus species. Two strains that proved to be the most efficient in degrading crude oil was Aspergillus niger (54%) and Penicillium commune (48%) Subsequent to two weeks of cultivation in BHS medium the degradation rate were recorded by using spectrophotometer and GC-MS. Hence, it is cleared that these fungal strains has capability of degradation and can be utilize for cleaning the Saudi Arabian environment.

Keywords: Fungal strains, hydrocarbon contaminants, molecular identification, biodegradation, GC-MS.

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Authors: S.K.Kavitha, P.N.Palanisamy

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The accelerated sonophotocatalytic degradation of Reactive Red (RR) 120 dye under visible light using dye sensitized TiO2 activated by ultrasound has been carried out. The effect of sonolysis, photocatalysis and sonophotocatalysis under visible light has been examined to study the influence on the degradation rates by varying the initial substrate concentration, pH and catalyst loading to ascertain the synergistic effect on the degradation techniques. Ultrasonic activation contributes degradation through cavitation leading to the splitting of H2O2 produced by both photocatalysis and sonolysis. This results in the formation of oxidative species, such as singlet oxygen (1O2) and superoxide (O2 -●) radicals in the presence of oxygen. The increase in the amount of reactive radical species which induce faster oxidation of the substrate and degradation of intermediates and also the deaggregation of the photocatalyst are responsible for the synergy observed under sonication. A comparative study of photocatalysis and sonophotocatalysis using TiO2, Hombikat UV 100 and ZnO was also carried out.

Keywords: Photocatalysis, Reactive Red 120, Sonophotocatalysis, Sonolysis.

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Authors: T. C. Machaba, S. M. Mahlo

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The current study investigates the antifungal properties of crude plant extracts from selected medicinal plant species. Eight plant species used by the traditional healers and local people to treat fungal infections were selected for further phytochemical analysis and biological assay. The selected plant species were extracted with solvent of various polarities such as acetone, methanol, ethanol, hexane, dichloromethane, ethyl acetate and water. Leaf, roots and bark extracts of Maerua juncea Pax, Albuca seineri (Engl & K. Krause) J.C Manning & Goldblatt, Senna italica Mill., Elephantorrhiza elephantina (Burch.) Skeels, Indigofera circinata Benth., Schinus molle L., Asparagus buchananii Bak., were screened for antifungal activity against three animal fungal pathogens (Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans). All plant extracts were active against the tested microorganisms. Acetone, dichloromethane, hexane and ethanol extracts of Senna italica and Elephantorrhiza elephantine had excellent activity against Candida albicans and A. fumigatus with the lowest MIC value of 0.02 mg/ml. Bioautography assay was used to determine the number of antifungal compounds presence in the plant extracts. No active compounds were observed in plant extracts of Indigofera circinnata, Schinus molle and Pentarrhinum insipidum with good antifungal activity against C. albicans and A. fumigatus indicating possible synergism between separated metabolites.

Keywords: Antifungal activity, minimum inhibitory concentration, bioautography.

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Authors: Ákos Malatinszky, Szilvia Ádám

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Climate change causes severe effects on natural habitats, especially wetlands. These challenges require the adaptation of their management to probable effects of climate change. A compilation of necessary changes in land management was collected in a Hungarian area being both national park and Natura 2000 SAC and SCI site in favor of increasing the resilience and reducing vulnerability. Several factors, such as ecological aspects, nature conservation and climatic adaptation should be combined with social and economic factors during the process of developing climate change adapted management on vulnerable wetlands. Planning adaptive management should be determined by a priority order of conservation aims and evaluation of factors at the determined planning unit. Mowing techniques, frequency and exact date should be observed as well as grazing species and their breed, due to different grazing, group forming and trampling habits. Integrating landscape history and historical land development into the planning process is essential.

Keywords: Adaptation, climate change, management, wetland.

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Authors: Vassilena Dakova, Mariana Panayotova-Pencheva

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The present work focused on the study of pulmonary helminth-fauna of the stone marten in Bulgaria in terms of which the data are little. For the purpose, four stone martens were helminthologically necropsied according to the common technique. In addition, some of the injured lung parts were investigated after their boiling in lactic acid and subsequent compression. Four nematode species from different families of order Strongylida and Trichocephalida were found in the lungs. These were Crenosoma petrowi Morosov, 1939; Eucoleus aerophilus Creplin, 1839; Filaroides martis Werner, 1782 and Sobolevingylus petrowi Romanov, 1952. Some of the parasite structures with taxonomic importance were measured and described. According to our best knowledge, the species F. martis and S. petrowi are recorded for the first time as a part of the helminth-fauna of Southeast Europe and Bulgaria in particular.

Keywords: Bulgaria, Crenosoma petrowi, Eucoleus aerophilus, Filaroides martis, lung parasites, Sobolevingylus petrowi, stone martens.

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Authors: Hasan Kalyoncu, Ayşe Güneş

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This study was carried on 12 determined stations, on Alara and Karpuz Streams, between January and November 2014. Seasonal samples were taken from the stations to analyze physicochemical parameters and Plecoptera Fauna in the water. The correlation between identified taxa and physicochemical data were tried to determine. As the result of the study, 2088 individuals from Plecoptera fauna were examined, 3 genera and 13 species were identified. The taxa of Brachyptera risi, Capnia bifrons, Dinocras cephalotes, Diura bicaudata, Isogenus nebecula, Isogenus sp., Isoperla grammatica, Leuctra hippopus, Leuctra inermis, Leuctra moselyi, Leuctra sp., Nemoura sp., Perla bipunctata, Perla marginata, Protonemura meyeri and Rhabdiopteryx acuminata were determined. In Alara Stream, the dominant species were; Isogenus nebecula at stations I and IV, Leuctra moselyi at station II, Leuctra hippopus at stations III, V and VI. In Karpuz Stream, Brachyptera risi was the dominant species in all stations. While Leuctra hippopus was the dominant taxon in Alara Stream, in Karpuz Stream it was Brachyptera risi. The highest diversity value was at station III and the lowest was at station VI in Alara Stream and the lowest diversity value was at station VI, while the highest was at station I in Karpuz Stream. In Alara Stream, the most similar stations were I and III, while in Karpuz Stream the highest similarity was determined between stations I and II. As for the evaluation result, the water quality of Alara and Karpuz Streams were determined as at oligosaprobic level.

Keywords: Alara Stream, Karpuz Stream, Plecoptera, water quality.

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Authors: Mansour Al Qubeissi, Sergei S. Sazhin, Cyril Crua, Morgan R. Heikal

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This paper presents the application of the Discrete Component Model for heating and evaporation to multi-component biodiesel fuel droplets in direct injection internal combustion engines. This model takes into account the effects of temperature gradient, recirculation and species diffusion inside droplets. A distinctive feature of the model used in the analysis is that it is based on the analytical solutions to the temperature and species diffusion equations inside the droplets. Nineteen types of biodiesel fuels are considered. It is shown that a simplistic model, based on the approximation of biodiesel fuel by a single component or ignoring the diffusion of components of biodiesel fuel, leads to noticeable errors in predicted droplet evaporation time and time evolution of droplet surface temperature and radius.

Keywords: Heat/Mass Transfer, Biodiesel, Multi-component Fuel, Droplet, Evaporation.

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Authors: Petruzzelli G., Pedron F., Rosellini I., Tassi E., Gorini F., Barbafieri M.

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This work involved the use of phytoremediation to remediate an aged soil contaminated with polychlorinated biphenyls (PCBs). At microcosm scale, tests were prepared using soil samples that have been collected in an industrial area with a total PCBs concentration of about 250 μg kg-1. Medicago sativa and Lolium italicum were the species selected in this study that is used as “feasibility test" for full scale remediation. The experiment was carried out with the addition of a mixture of randomly methylatedbeta- cyclodextrins (RAMEB). At the end of the experiment analysis of soil samples showed that in general the presence of plants has led to a higher degradation of most congeners with respect to not vegetated soil. The two plant species efficiencies were comparable and improved by RAMEB addition with a final reduction of total PCBs near to 50%. With increasing the chlorination of the congeners the removal percentage of PCBs progressively decreased.

Keywords: contaminated soil, feasibility test, phytoremediation, polychlorinated biphenyls

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Authors: Usha Chouhan, K. R. Pardasani

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Phylogenies ; The evolutionary histories of groups of species are one of the most widely used tools throughout the life sciences, as well as objects of research with in systematic, evolutionary biology. In every phylogenetic analysis reconstruction produces trees. These trees represent the evolutionary histories of many groups of organisms, bacteria due to horizontal gene transfer and plants due to process of hybridization. The process of gene transfer in bacteria and hybridization in plants lead to reticulate networks, therefore, the methods of constructing trees fail in constructing reticulate networks. In this paper a model has been employed to reconstruct phylogenetic network in honey bee. This network represents reticulate evolution in honey bee. The maximum parsimony approach has been used to obtain this reticulate network.

Keywords: Hybridization, HGT, Reticulate networks, Recombination, Species, Parsimony.

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Authors: R. Hegedűs, É. Kerepeczki, D. Gál, F. Pekár, M. Oncsik Bíróné, Gy. Lakatos

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The growth of the aquaculture industry has been associated with negative environmental impacts through the discharge of raw effluents into the adjacent receiving water bodies. Macrophytes from natural saline lakes, which have adaptability to the high salinity, can be suitable for saline effluent treatment. Eight emergent species from natural saline area were planted in an experimental gravel bed hydroponic mesocosm (GBH) which was treated with effluent water from an intensive fish farm using geothermal water. In order to examine the applicability of the halophytes in treatment processes, we tested the relative efficacy of total nitrogen (TN), total phosphorus (TP), potassium (K), sodium (Na), magnesium (Mg) and calcium (Ca) removal for the saline wastewater treatment. Four of the eight species, which were Phragmites australis, Typha angustifolia, Glyceria maxima, Scirpus lacustris spp. tabernaemontani could survive and contribute the experimental treatment.

Keywords: Gravel bed hydroponic system, halophytes, intensive fish farm, salt removal

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Authors: Sherif S. Z. Hindi

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Wood pyrolysis for Casuarina glauca, Casuarina cunninghamiana, Eucalyptus camaldulensis, Eucalyptus microtheca was made at 450°C with 2.5°C/min. in a flowing N2-atmosphere. The Eucalyptus genus wood gave higher values of specific gravity, ash , total extractives, lignin, N2-liquid trap distillate (NLTD) and water trap distillate (WSP) than those for Casuarina genus. The GHC of NLTD was higher for Casuarina genus than that for Eucalyptus genus with the highest value for Casuarina cunninghamiana. Guiacol, 4-ethyl-2-methoxyphenol and syringol were observed in the NLTD of all the four wood species reflecting their parent hardwood lignin origin. Eucalyptus camaldulensis wood had the highest lignin content (28.89%) and was pyrolyzed to the highest values of phenolics (73.01%), guaiacol (11.2%) and syringol (32.28%) contents in methylene chloride fraction (MCF) of NLTD. Accordingly, recoveries of syringol and guaiacol may become economically attractive from Eucalyptus camaldulensis.

Keywords: Wood, Pyrolysis, Guaiacol, Syringol

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Authors: Supanyika Sengsai, Aree Thongpukdee, Chalermchai Kanchanakachen

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The study aimed to collect morphological data of secretory structures that contribute to taxonomy of Indigofera. Detail features of trichomes occurrence in vegetative and reproductive organs of Indigofera wightii Grah. ex Wigh & Arn., a species traditionally used as source of indigo to dye “Thaisongdam” clothing were investigated. Examination through light microscopy and scanning electrom microscopy were done. Non secretory, T-shaped trichomes appeared throughout surfaces of stems, leaves, flowers and fruits. Secretory or glandular trichomes occurred in two types; one has big cylindrical head and short peduncle, distributed on adaxial surface of sepals and around the pedicel, whereas another possesses smaller cylindrical head but long peduncle. The latter was found on apical surface of immature pods. No phenolic and lipophilic compounds were detected from these glands.

Keywords: Indigofera, Thailand, Thaisongdam, trichome.

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Authors: Behrouzinia P., Irani R. A., Saidi M.H.

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In this paper, a one-dimensional numerical approach is used to study the effect of applying electrohydrodynamics on the temperature and species mass fraction profiles along the microcombustor. Premixed mixture is H2-Air with a multi-step chemistry (9 species and 19 reactions). In the micro-scale combustion because of the increasing ratio of area-to-volume, thermal and radical quenching mechanisms are important. Also, there is a significant heat loss from the combustor walls. By inserting a number of electrodes into micro-combustor and applying high voltage to them corona discharge occurs. This leads in moving of induced ions toward natural molecules and colliding with them. So this phenomenon causes the movement of the molecules and reattaches the flow to the walls. It increases the velocity near the walls that reduces the wall boundary layer. Consequently, applying electrohydrodynamics mechanism can enhance the temperature profile in the microcombustor. Ultimately, it prevents the flame quenching in microcombustor.

Keywords: micro-combustor, electrohydrodynamics, temperature profile, wall quenching

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Authors: B. Chouhan, A. Denesyuk, J. Heino, M. S. Johnson, K. Denessiouk

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Integrins are a large family of multidomain α/β cell signaling receptors. Some integrins contain an additional inserted I domain, whose earliest expression appears to be with the chordates, since they are observed in the urochordates Ciona intestinalis (vase tunicate) and Halocynthia roretzi (sea pineapple), but not in integrins of earlier diverging species. The domain-s presence is viewed as a hallmark of integrins of higher metazoans, however in vertebrates, there are clearly three structurally-different classes: integrins without I domains, and two groups of integrins with I domains but separable by the presence or absence of an additional αC helix. For example, the αI domains in collagen-binding integrins from Osteichthyes (bony fish) and all higher vertebrates contain the specific αC helix, whereas the αI domains in non-collagen binding integrins from vertebrates and the αI domains from earlier diverging urochordate integrins, i.e. tunicates, do not. Unfortunately, within the early chordates, there is an evolutionary gap due to extinctions between the tunicates and cartilaginous fish. This, coupled with a knowledge gap due to the lack of complete genomic data from surviving species, means that the origin of collagen-binding αC-containing αI domains remains unknown. Here, we analyzed two available genomes from Callorhinchus milii (ghost shark/elephant shark; Chondrichthyes – cartilaginous fish) and Petromyzon marinus (sea lamprey; Agnathostomata), and several available Expression Sequence Tags from two Chondrichthyes species: Raja erinacea (little skate) and Squalus acanthias (dogfish shark); and Eptatretus burgeri (inshore hagfish; Agnathostomata), which evolutionary reside between the urochordates and osteichthyes. In P. marinus, we observed several fragments coding for the αC-containing αI domain, allowing us to shed more light on the evolution of the collagen-binding integrins.

Keywords: Integrin αI domain, integrin evolution, collagen binding, structure, αC helix

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Authors: C. Phalakornkule, W. Worachai, T. Satitayut

Abstract:

The electrochemical coagulation of a kaolin suspension was investigated at the currents of 0.06, 0.12, 0.22, 0.44, 0.85 A (corresponding to 0.68, 1.36, 2.50, 5.00, 9.66 mA·cm-2, respectively) for the contact time of 5, 10, 20, 30, and 50 min. The TSS removal efficiency at currents of 0.06 A, 0.12 A and 0.22 A increased with the amount of iron generated by the sacrificial anode, while the removal efficiencies did not increase proportionally with the amount of iron generated at the currents of 0.44 and 0.85 A, where electroflotation was clearly observed. Zeta potential measurement illustrated the presence of the highly positive charged particles created by sorption of highly charged polymeric metal hydroxyl species onto the negative surface charged kaolin particles at both low and high applied currents. The disappearance of the individual peaks after certain contact times indicated the attraction between these positive and negative charged particles causing agglomeration. It was concluded that charge neutralization of the individual species was not the only mechanism operating in the electrocoagulation process at any current level, but electrostatic attraction was likely to co-operate or mainly operate.

Keywords: Coagulation, Electrocoagulation, Electrostatics, Suspended solids, Zeta potential

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Authors: Deepak Mohan, Sushma Sharma

Abstract:

Diclofenac sodium, a member of the acetic acid family of non-steroidal anti-inflammatory drugs, is used to retard inflammation, arthritis pain and ankylosing spondylitis. The drug is known to cause severe injury in different tissues due to formation of reactive oxygen species. The present study is focused on the effect of different doses of diclofenac (4 mg/kg/body weight and 14 mg/kg/body weight on histoarchitecture of the liver from 7-28 days of the investigation. Diclofenac administration resulted in distorted hepatic degeneration and formation of wide areas in the form of sinusoidal gaps. Hepatic fibrosis noticed in different stages of investigation could be attributed to chronic inflammation and reactive oxygen species which results in deposition of extracellular matrix proteins. The abrupt degenerative changes observed during later stages of the experiment showed maximum damage to the liver, and there was enlargement of sinusoidal gaps accompanied by maximum necrosis in the tissues.

Keywords: Arthritis, diclofenac, histoarchitecture, sinusoidal.

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Authors: Ming-Hua Fu

Abstract:

No negative control nor control to prevent microbes from escaping was set when the S-shaped flask experiments were performed by Pasteur. Microscope was not used to observe the media in the flasks. Louis Pasteur’s S-shaped flask experiment was re-examined by using U-shaped flasks, modified S-shaped flasks and microscope. A mixture of microbes was isolated from the room air, from which one rod-shaped Bacillus species with proposed name Bacillus gaso-mobilis sp nov and one grape-shaped Staphylococcus species with proposed name of Staphylococcus gaso-mobilis sp nov were identified. Their penicillin and ampicillin resistant strains containing plasmids were isolated. These bacteria could change color, produce odor and automatically move in the air. They did not form colonies on solid media. They had a high suspension capacity in liquid media. Their light absorbance peaked at the wave length of 320 nm.  It was concluded that there were flaws with Louis Pasteur’s S-shaped flask experiments. 

Keywords: Bacteria, gaso-mobile, re-examine, S-shaped flasks.

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Authors: S. Rasekhinia, S. Aghanajafizadeh, K. Eslami

Abstract:

We studied the selection of nest sites by Persian ground Jay (Podoces pleskei), in a semi -desert central Iran. Habitat variables such as plant species number, height of plant species, vegetation percent and distance to water sources of nest sites were compared with randomly selected non- used sites. The results showed that the most important factors influencing nesting site selection were total vegetation percent and number of shrubs (Zgophyllum eurypterum and Atraphaxis spinosa). The mean vegetation percent of 20 area selected by Persian Ground Jay was (4.41+ 0.17), which was significantly larger than that of the non – selected area (2.08 + 0.06). The number of Zygophyllum eurypterum (1.13+ 0.01) and Atraphaxis spinosa (1.36+ 0.10) were also significantly higher compared with the control area (0.43+ 0.07) and (0.58+ 0.9) respectively.

Keywords: Persian Ground Jay, Habitat variables, Iran.

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Authors: Azita Behbahaninia, Seid Ahmad Mirbagheri

Abstract:

The use of sewage sludge and effluents from wastewater treatment plants for irrigation of agricultural lands is on the rise particularly in peri-urban areas of developing countries. The reuse of nutrients and organic matter in treated wastewater and sewage sludge via land application is a desirable goal. However, trace or heavy metals present in sludge pose the risk of human or phytotoxicity from land application. Long-term use of sewage sludge, heavy metals can accumulate to phytotoxic levels and results in reduced plants growth and/or enhanced metal concentrations in plants, which consumed by animals then enter the food chain. In this research, the amount of heavy metals was measured in plants irrigated with wastewater and sludge application. For this purpose, three pilots were made in a Shush treatment plant in south of Tehran. Three plants species, spinach, lettuce and radish were selected and planted in the pilots.First pilot was irrigated just with wastewater of treatment plant and second pilot was irrigated with wastewater and sludge application .Third pilot was irrigated with simulated heavy metals solution equal 50 years of irrigation. The results indicate that the average of amount of heavy metals Pb, Cd in three plant species in first pilot were lower than permissible limits .In second pilot, Cadmium accumulations are high in three species plants and more than the standard limits. Concentration of Cd , Pb have exceed their permitted limits in plants in third pilot . It was concluded that the use of wastewater and sludge application in agricultural lands enriched soils with heavy metals to concentrations that may pose potential environmental and health risks in the long-term.

Keywords: Soil, contaminate, heavy metals, wastewater, sludge, plants.

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