The Occurrence of Fungi in Activated Sludge from MBRs

Authors: Mohamed F. Awad, M. Kraume

Abstract:

The objective of this study is to evaluate the occurrence of fungi in aerobic and anoxic activated sludge from membrane bioreactors (MBRs). Thirty-six samples of both aerobic and anoxic activated sludge were taken from 2 MBR treating domestic wastewater. Over a period of eight months 2 samples from each plant were taken per month. The samples were prepared for count and definition of fungi. The obtained data show that, sixty species belonging to 27 genera were collected from activated sludge samples under aerobic and anoxic conditions. Regarding to the fungi definition, under aerobic condition the Geotrichum was found at (8.8%) followed by Penicillium (75.0%), Yeasts (65.7%) and Trichoderma (55.5%), while Yeasts (77.1%) Geotrichum candidumand Penicillium (61.1%) species were the most prevalent in anoxic activated sludge. The results indicate that activated sludge is habitat for growth and sporulation of different groups of fungi, both saprophytic and pathogenic.

Keywords: Aerobic conditions, Anoxic conditions, Activated sludge, Membrane bioreactor, Fungi.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1058217

References:

[1] F. Bux and H. C. A. Kasan. Microbiological survey of ten activated sludge plants. Water SA 20 (1): 61-72. 1994.
[2] A. I. I. Abdel-Hafez and H. M. M. EL-Sharouny, The occurrence of keratoinophilic fungi in sewage sludge from Egypt. Journal of basic microbiology 30:73-79. 1990.
[3] D. H. Eikelboom. Process control of activated sludge plants by mycroscopic investigation. 156p. ISBN: 9781900222297. 2000.
[4] M. T. Hedayati, M. Mirzakhani. Survey of keratinophilic fungi in sewage sludge from wastewater treatment plants of Mazandaran, Islamic Republic of Iran. Eastern Mediterranean Health Journal, 15(2):451- 454. 2009.
[5] D. Jenkins, M. G. Richard and G. T. Daigger. Manual on the causes and control of activated sludge bulking and foaming. Water research commission, Pretoria. 1986.
[6] A. D. Adamse, M. H. Deinemad and A. J. B. Zender. Studies on bacterial activities in aerobic and anaerobic waste water purification. Antonie van Leeuwenhoek 50:665-682. 1984.
[7] J. Häuslerova. The growth of micromycetes in activated sludge media. Acta hydrochim. Hydrobiol 4(2):137-152. 1979.
[8] T. Watanabe. Pictorial atlas of soil and seed fungi.Morphologies of cultured fungi and key to species.2nd, CRC, Press, Boca Raton, London, New York, Washington, D.C. 486 p. 2002 .
[9] A. H. Moubasher. Soil fungi of Qatar and other Arab Countries. The Scientific and Applied Research Centre, University of Qatar, Doha, Qatar. 566 p. 1993.
[10] K. H. Domsch, W. Gams and T. Anderson. Compendium of Soil Fungi. Academic Press. London.-San Francisco. 1980.
[11] D. Frey, R. J. Oldfield and R. C. Bridger. A Colour Atlas of Pathogenic Fungi. London, Wolfe Medical Publ. 1979.
[12] C. Booth. Fusarium Laboratory Guide to the Identification of Major Species. Commonwealth Mycological Institute, Key, Surrey, England.1977.
[13] M. B. Ellis MB. Dematiaceous Hyphomycetes Commonwealth Mycological Institute, Kew, Surrey, England. 1971.
[14] K. B. Raper, I. Fenneldl. The Genus.Aspergillus. Williams and Wilkins. Baltimore, U.S.A. 1965.
[15] M. Kacprzak, E. Neczaj and E. Okoniewska. The comparative mycological analysis of wastewater and sewage sludge from selected wastewater treatment plants. Desalination 185: 363-370. 2005.
[16] K. Ulfig. Studies of keratinolytic and keratinophilic fungi in sewage sludge by means of a multi-temperature hair baiting method. Polish Journal of Environmental Studies 12(4):461-466. 2003.
[17] K. Ulfig. Sludge liming decreases the growth of keratinolytic and Keratinophilic fungi. Polish Journal of Environmental Studies 15 (2):341-346. 2006.
[18] K. Ulfig and M. Korcz. Isolation of keratinophilic fungi from sewage sludge. Sabouraudia, 21:247-250. 1983.
[19] K. Ulfig and M. Korcz. Keratinophilic fungi in sewage sludge applied to devastate urban soil. A preliminary experiment. International journal of environmental health research 4:244-253. 1994.
[20] C. G. Kahn, P. Stegmann, H. C. Kasan and A. A. W. Baecker. The influence of altered anticorrosion treatment on the microflora of activated sludge in petrochemical plant effluent. Water SA 16 (1): 23-28. 1990.
[21] K. Ulfig, M. Terakowski, G. Plaza and O. Kosarewicz. Keratinolytic fungi in sewage sludge. Mycopathologia 136:41-46. 1996.
[22] N. Kunichika, F. Tohru and I. Kazuei. Isolation of a fungus from denitrifying activated sludge that degrades highly chlorinated dioxions. J Mater Cycles Waste Manag 4: 127-134. 2002.
[23] A. I. I. Abdel-Hafez and H. M. M. EL-Sharouny. Seasonal fluctuations of fungi in Egyptian soil receiving city sewage effluents.Cryptogamie, Mycol. 8 (3):235-249. 1987.
[24] A. I. I. Abdel-Hafez, M. M. K. Bagy and A. A. M. Shoreit, Keratinolytic fungi in mud of Ibrahimia canal, Egypt. Cryptogamie, Mycol. (in press). 1989.
[25] L. C. Warren and L. S. Ronald LS. Isolation of Candida albicans from Freshwater and Sewage, p. 840-842. 1981.
[26] P. A. Thomas. Current perspectives on ophthalmic mycoses. Clin Microbiol Rev. 16(4):730-797. 2003.
[27] M. D. Richardson and B. Elewski. Fast Facts: Superficial Fungal Infections. Health Press, Oxford. 2000.
[28] H. Velez and F. Diaz. Onychomycosis due to saprophytic fungi. Mycopathologia, 91:87-92. 1985.
[29] J. W. Rippon. Medical Mycology. W. B. Sanders Company, Philadelphia. 1982.