Search results for: Wheat genotypes.
204 Polymorphism of HMW-GS in Collection of Wheat Genotypes
Authors: M. Chňapek, M. Tomka, R. Peroutková, Z. Gálová
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Processes of plant breeding, testing and licensing of new varieties, patent protection in seed production, relations in trade and protection of copyright are dependent on identification, differentiation and characterization of plant genotypes. Therefore, we focused our research on utilization of wheat storage proteins as genetic markers suitable not only for differentiation of individual genotypes, but also for identification and characterization of their considerable properties. We analyzed a collection of 102 genotypes of bread wheat (Triticum aestivum L.), 41 genotypes of spelt wheat (Triticum spelta L.), and 35 genotypes of durum wheat (Triticum durum Desf.), in this study. Our results show, that genotypes of bread wheat and durum wheat were homogenous and single line, but spelt wheat genotypes were heterogenous. We observed variability of HMW-GS composition according to environmental factors and level of breeding and predict technological quality on the basis of Glu-score calculation.
Keywords: Genotype identification, HMW-GS, wheat quality.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2385203 Assessment of Resistance of Wheat Genotypes (T. aestivum and T. durum) To Boron Toxicity
Authors: M. Rüştü Karaman, Mehmet Zengin, Ayhan Horuz
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Research on the boron (B) toxicity problems had recently considerable relation, especially in the dry regions of the world. Development of resistant varieties to B toxicity is a high priority on these regions, where the soils have high levels of B. Thus, this study aimed to assessment the resistance of wheat genotypes to B toxicity using the agronomic and physiologic parameters. For this aim, a pot experiment, based on a completely randomized design with three replications, was conducted using the soil of calcareous usthochrepts. In the study, twenty different wheat genotypes of T. aestivum and T. Durum were used. Boron fertilizer at the levels of 0 (-B), 30 mg B kg-1 (+B) as H3BO3 was applied to the pots. After harvest, plant dry matter yield was recorded, and total B concentrations in tops of wheat plants were determined. The results have revealed the existence of a large genotypic variation among wheat genotypes to their physiologic and agronomic susceptibility to B toxicity.
Keywords: Boron, B toxicity, B uptake, wheat genotypes.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1449202 Study of Water Relations, Chlorophyll and their Correlations with Grain Yield in Wheat(Triticum aestivum L.) Genotypes
Authors: Mokhtar Ghobadi, Saeed Khosravi, Danial Kahrizi, Firooz Shirvani
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The objective of this experiment was to study of water relations and chlorophyll in different wheat genotypes and their correlations with grain and biological yields. 21 genotypes of bread wheat were compared in a field experiment as randomized complete blocks design with four replications. The results showed that relative water deficit, relative water loss, excised leaf water retention, cell membrane stability, chlorophyll-a, chlorophyll-b, total chlorophyll, grain yield and biological yield were different significantly among wheat genotypes, but SPAD-chlorophyll index, relative water content and chlorophyll florescence were not. Significant correlations were not observed among above mentioned water relations and chlorophyll characteristics with grain yield, but there was a positive and significant correlation between biological yield and grain yield.Keywords: Wheat, water relations, chlorophyll, yield
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2420201 Evaluation of drought Tolerance Indices in Dryland Bread wheat Genotypes under Post-Anthesis drought Stress
Authors: Mokhtar Ghobadi , Mohammad-Eghbal Ghobadi, Danial Kahrizi, Alireza Zebarjadi, Mahdi Geravandi
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Post-anthesis drought stress is the most important problem affecting wheat production in dryland fields, specially in Mediterranean regions. The main objective of this research was to evaluate drought tolerance indices in dryland wheat genotypes under post-anthesis drought stress. The research was including two different experiments. In each experiment, twenty dryland bread wheat genotypes were sown in a randomized complete blocks design (RCBD) with three replications. One of experiments belonged to rain-fed conditions (post-anthesis drought stress) and other experiment was under non-stress conditions (with supplemental irrigation). Different drought tolerance indices include Stress Tolerance (Tol), Mean Productivity (MP), Geometric Mean Productivity (GMP), Stress Susceptibility Index (SSI), Stress Tolerance Index (STI), Harmonic Mean (HAM), Yield Index (YI) and Yield Stability Index (YSI) were evaluate based on grain yield under rain-fed (Ys) and supplemental irrigation (Yp) environments. G10 and G12 were the most tolerant genotypes based on TOL and SSI. But, based on MP, GMP, STI, HAM and YI indices, G1 and G2 were selected. STI, GMP and MP indices had high correlation with grain yield under rain-fed and supplementary irrigation conditions and were recognized as appropriate indices to identify genotypes with high grain yield and low sensitivity to drought stress environments.Keywords: Dryland wheat, Supplemental irrigation, Tolerance indices
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2051200 Induction of alpha-Amylase in Wheat Grain Cultivars as an Indicator of Resistance to Pre-harvest Sprouting
Authors: Aidar A. Khakimzhanov, Vladimir A. Kuzovlev, Nurgul S. Mamytova, Dinara A. Shansharova, Oleg V. Fursov
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The influence of humidity and low temperature on the α- amylase activity and isoenzyme composition of grains of different wheat varieties have been studied. The identified samples of varieties have significant difference in the level of enzyme induction under the impact of high humidity and low temperature. It is proposed to use this methodological approach for testing genotypes and wheat breeding lines for resistance to pre-harvest sprouting (PHS).
Keywords: α-Amylase, isoenzymes, wheat, pre-harvest sprouting.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1852199 Screening Wheat Parents of Mapping Population for Heat and Drought Tolerance, Detection of Wheat Genetic Variation
Authors: H.R. Balouchi
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To evaluate genetic variation of wheat (Triticum aestivum) affected by heat and drought stress on eight Australian wheat genotypes that are parents of Doubled Haploid (HD) mapping populations at the vegetative stage, the water stress experiment was conducted at 65% field capacity in growth room. Heat stress experiment was conducted in the research field under irrigation over summer. Result show that water stress decreased dry shoot weight and RWC but increased osmolarity and means of Fv/Fm values in all varieties except for Krichauff. Krichauff and Kukri had the maximum RWC under drought stress. Trident variety was shown maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield and Fv/Fm 0.815 under water stress condition. However, the recovery of quantum yield was apparent between 4 to 7 days after stress in all varieties. Nevertheless, increase in water stress after that lead to strong decrease in quantum yield. There was a genetic variation for leaf pigments content among varieties under heat stress. Heat stress decreased significantly the total chlorophyll content that measured by SPAD. Krichauff had maximum value of Anthocyanin content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b (0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content belonged to Kukri. The quantum yield of all varieties decreased significantly, when the weather temperature increased from 28 ÔùªC to 36 ÔùªC during the 6 days. However, the recovery of quantum yield was apparent after 8th day in all varieties. The maximum decrease and recovery in quantum yield was observed in Krichauff. Drought and heat tolerant and moderately tolerant wheat genotypes were included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut and Excalibur were clustered into most sensitive and moderately sensitive genotypes. Finally, the results show that there was a significantly genetic variation among the eight varieties that were studied under heat and water stress.Keywords: Abiotic stress, Genetic variation, Fluorescence, Wheat genotypes.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2227198 Screening Wheat Parents of Mapping Population for Heat and Drought Tolerance, Detection of Wheat Genetic Variation
Authors: H.R. Balouchi
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To evaluate genetic variation of wheat (Triticum aestivum) affected by heat and drought stress on eight Australian wheat genotypes that are parents of Doubled Haploid (HD) mapping populations at the vegetative stage, the water stress experiment was conducted at 65% field capacity in growth room. Heat stress experiment was conducted in the research field under irrigation over summer. Result show that water stress decreased dry shoot weight and RWC but increased osmolarity and means of Fv/Fm values in all varieties except for Krichauff. Krichauff and Kukri had the maximum RWC under drought stress. Trident variety was shown maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield and Fv/Fm 0.815 under water stress condition. However, the recovery of quantum yield was apparent between 4 to 7 days after stress in all varieties. Nevertheless, increase in water stress after that lead to strong decrease in quantum yield. There was a genetic variation for leaf pigments content among varieties under heat stress. Heat stress decreased significantly the total chlorophyll content that measured by SPAD. Krichauff had maximum value of Anthocyanin content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b (0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content belonged to Kukri. The quantum yield of all varieties decreased significantly, when the weather temperature increased from 28 ÔùªC to 36 ÔùªC during the 6 days. However, the recovery of quantum yield was apparent after 8th day in all varieties. The maximum decrease and recovery in quantum yield was observed in Krichauff. Drought and heat tolerant and moderately tolerant wheat genotypes were included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut and Excalibur were clustered into most sensitive and moderately sensitive genotypes. Finally, the results show that there was a significantly genetic variation among the eight varieties that were studied under heat and water stress.
Keywords: Abiotic stress, genetic variation, fluorescence, wheat genotypes.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2586197 Growth and Stomatal Responses of Bread Wheat Genotypes in Tolerance to Salt Stress
Authors: Afrasyab Rahnama, Kazem Poustini, Reza Tavakkol-Afshari, Afshin Tavakoli
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Plant growth is affected by the osmotic stress as well as toxicity of salt in leaves. In order to study of salt stress effects on stomatal conductance and growth rate and relationship between them as wells osmotic and Na+-specific effects on these traits, four bread wheat genotypes differing in salt tolerance were selected. Salinity was applied when the leaf 4 was fully expanded. Sodium (Na+) concentrations in flag leaf blade at 3 salinity levels (0, 100 and 200 mM NaCl) were measured. Salt-tolerant genotypes showed higher stomatal conductance and growth rate compared to salt-sensitive ones. After 10 and 20 days exposure to salt, stomatal conductance and relative growth rate were reduced, but the reduction was greater in sensitive genotypes. Growth rate was reduced severely in the first period (1-10 days) of salt commencements and it was due to osmotic effect of salt not Na+ toxicity. In the second period (11-20 days) after salt treatment growth reduced only when salt accumulated to toxic concentrations in the leaves. A positive relationship between stomatal conductance and relative growth rate showed that stomatal conductance can be a reliable indicator of growth rate, and finally can be considered as a sensitive indicator of the osmotic stress. It seems 20 days after salinity, the major effect of salt, especially at low to moderate salinity levels on growth properties was due to the osmotic effect of salt, not to Na+-specific effects within the plant.
Keywords: Osmotic stress, relative growth rate, stomatal conductance, wheat.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2285196 Investigation of Genetic Variation for Agronomic Traits among the Recombinant Inbred Lines of Wheat from the Norstar × Zagross Cross under Water Stress Condition
Authors: Mohammad Reza Farzami Pour
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Determination of genetic variation is useful for plant breeding and hence production of more efficient plant species under different conditions, like drought stress. In this study a sample of 28 recombinant inbred lines (RILs) of wheat developed from the cross of Norstar and Zagross varieties, together with their parents, were evaluated for two years (2010-2012) under normal and water stress conditions using split plot design with three replications. Main plots included two irrigation treatments of 70 and 140 mm evaporation from Class A pan and sub-plots consisted of 30 genotypes. The effect of genotypes and interaction of genotypes with years and water regimes were significant for all characters. Significant genotypic effect implies the existence of genetic variation among the lines under study. Heritability estimates were high for 1000 grain weight (0.87). Biomass and grain yield showed the lowest heritability values (0.42 and 0.50, respectively). Highest genotypic and phenotypic coefficients of variation (GCV and PCV) belonged to harvest index. Moderate genetic advance for most of the traits suggested the feasibility of selection among the RILs under investigation. Some RILs were higher yielding than either parent at both environments.Keywords: Wheat, genetic gain, heritability, recombinant inbred lines.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2334195 Some Yield Parameters of Wheat Genotypes
Authors: Shatha A. Yousif, Hatem Jasim, Ali R. Abas, Dheya P. Yousef
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To study the effect of the cross direction in bead wheat, three hybrid combinations (Babyle 113, Iratome), (Sawa, Tamose2) and (Al Hashymya, Al Iraq) were tested for plant height, spike and awn length, number of grains per spike, 1000-grain weight, number of tillers/m and grain yield. The results revealed that the direction of the crosses significantly effect on the number of grains/spike, number of tillers/m and grain yields. Grain yield was positively and significantly correlated with 1000-grain weight, number of grains per spike and tillers. Depend on the results of heritability and genetic advance it was suggested that 1000-grain weight, number of grains per spike and tillers should be given emphasis for future wheat yield improvement programs.
Keywords: Correlation, Genetic Advance, Heritability, Wheat, Yield Traits.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2641194 The Effects of Crop Rotation and Nutrient Supply on the Leaf Area Values of Winter Wheat in a Long-Term Experiment
Authors: Gergely Szilágyi, Péter Pepó
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Our field experiments were set at the RISF Látókép Experimental Farm of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen, on lime-coated chernozem soil. During our studies, we have investigated two winter wheat varieties (GK Öthalom, Mv Csárdás) of different genotypes. The preceding crops were sunflower and grain maize. We examined wheat leaf area index (LAI) five times during by BBCH scale. We have found that during the different stages of the vegetation period, the LAI values were different depending on the preceding crop, variety and nutrient levels. According to our results, the lowest LAI values were experienced in the control treatment, in the case of both preceding crops. According to our studies we can conclude that crop rotation and fertilizer treatment influenced the studied physiological trait to different extents.
Keywords: Winter wheat, crop rotation, fertilization, genotype, LAI.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2151193 Baking Quality of Hulled Wheat Species in Organic Farming
Authors: P. Konvalina, I. Capouchová, Z. Stehno
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The organic farmers use wider range of crop varieties than the conventional farming. Bread wheat is the most favorite and the most common food crop. The organic bread wheat is usually of worse technological quality. Therefore, it is supposed to be an attractive alternative to the hulled wheat species (einkorn, emmer wheat and spelt). Twenty-five hulled bread wheat varieties and control bread wheat ones were grown on the certified organic parcel in České Budějovice (the Czech Republic) between 2009 and 2012. Their baking quality was measured and evaluated with standard methods, and in accordance with ICC. The results have shown that the grain of hulled wheat varieties contain a lot of proteins in grains (up to 18 percent); even the organic hulled bread wheat varieties are characterized by such good baking quality. Einkorn and emmer wheat are of worse technological quality of proteins (low values of gluten index and Zeleny test), which is a disadvantage of these two wheat species. On the other hand, spelt wheat is of better technological quality and is similar to the control bread wheat varieties. Mixtures consisting of bread wheat, among others, are considered good alternatives; they may contribute to wider range of use of the hulled wheat species. It is one of the possibilities which may increase the proportion of proteins in bread wheat grains; the nutrition-rich hulled wheat grains may be also used in such way at the same time.
Keywords: Baking quality, organic farming, einkorn, emmer wheat, spelt.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1878192 A Tool for Creation Artificial Symbiotic Associations of Wheat
Authors: Zilya R. Vershinina, Andrei K. Baymiev, Aleksei K. Baymiev, Aleksei V. Chemeris
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This paper reports optimization of characteristics of bioballistic transformation of spring soft wheat (Triticum aestivum L. cultivar Raduga) and getting of transgenic plants, carrying pea lectin gene. This gene will let to create new associative wheat symbiosis with nodule bacteria of field pea, which has growth encouraging, fungistatic and other useful characteristics.Keywords: transgenic wheat, pea lectin, rhizobia root colonization, symbiosis
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1557191 Technological Value of Selected Spring Wheat Cultivars Depending on the Sowing Date
Authors: Marta Wyzińska, Jerzy Grabiński, Alicja Sułek
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The grain quality is a decisive factor in its use. In Poland, spring wheat is characterized by more favorable quality parameters in relation to the winter form of this species. In the present study, the effects of three different sowing dates (autumn, delayed autumn, and spring) and cultivar (Tybalt, Cytra, Bombona, Monsun, and Parabola) on the selected technological value parameters of spring wheat over three years were studied. The field trials were carried out in two locations (Bezek, Czesławice) in the Lubelskie Vivodeship, Poland. It was found that the falling number of spring wheat grains from autumn sowing dates was at a similar level to wheat sown in spring. The amount of wet gluten in the grain was variable in years, and its quality was better in wheat sown in spring. Sedimentation index was dependent upon on the cultivar.
Keywords: Sowing term, spring wheat, technological value, quality.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1174190 Modelling of Energy Consumption in Wheat Production Using Neural Networks “Case Study in Canterbury Province, New Zealand“
Authors: M. Safa, S. Samarasinghe
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An artificial neural network (ANN) approach was used to model the energy consumption of wheat production. This study was conducted over 35,300 hectares of irrigated and dry land wheat fields in Canterbury in the 2007-2008 harvest year.1 In this study several direct and indirect factors have been used to create an artificial neural networks model to predict energy use in wheat production. The final model can predict energy consumption by using farm condition (size of wheat area and number paddocks), farmers- social properties (education), and energy inputs (N and P use, fungicide consumption, seed consumption, and irrigation frequency), it can also predict energy use in Canterbury wheat farms with error margin of ±7% (± 1600 MJ/ha).
Keywords: Artificial neural network, Canterbury, energy consumption, modelling, New Zealand, wheat.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1416189 Carotenoids and Colour Before and After Storage of Organically and Conventionally Cultivated Potato Genotypes in Latvia
Authors: Irisa Murniece, Zanda Kruma, Ilze Skrabule
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Potatoes are a good source of carotenoids, which are lipophilic compounds synthesized in plastids from isoprenoids. The aim of this research was to determine the content of carotenoids in relationship with the colour of organically and conventionally cultivated potato genotypes before and after period of storage. In cooperation with the State Priekuli Plant Breeding Institute (Latvia), six potato genotypes were studied: 'Agrie dzeltenie', 'Prelma', 'Imanta', 'S-03135-10', 'S-99108-8' and 'S-01063-5'. All the genotypes were cultivated under three different conditions: organically and conventionally (two conditions). The content of carotenoids was determined by using spectrophotometer and the colour – L*a*b* system. The results of current research show that after the period of storage, carotenoid amount has increased and in conventionally cultivated potatoes it varies from 228.514 to 552.434 μg 100 g-1 while in organically cultivated potato genotypes – from 45.485 to 662.699 μg 100 g-1 FW. Colour of potato flesh was changing during storage.
Keywords: carotenoids, colour, organic and conventional cultivation, potato genotypes, storage
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1785188 Supercritical Carbon Dioxide Extraction of Phenolics and Tocopherols Enriched Oil from Wheat Bran
Authors: Kyung-Tae Kwon, Md. Salim Uddin, Go-Woon Jung, Jeong-Eun Sim, Byung-Soo Chun
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Supercritical carbon dioxide (SC-CO2) was used as a solvent to extract oil from wheat bran. Extractions were carried out in a semi-batch process at temperatures ranging from 40 to 60ºC and pressures ranging from 10 to 30 MPa, with a carbon dioxide (CO2) flow rate of 26.81 g/min. The oil obtained from wheat bran at different extraction conditions was quantitatively measured to investigate the solubility of oil in SC-CO2. The solubility of wheat bran oil was found to be enhanced in high temperature and pressure. The composition of fatty acids in wheat bran oil was measured by gas chromatography (GC). Linoleic, palmitic, oleic and γ-linolenic acid were the major fatty acids of wheat bran oil. Tocopherol contents in oil were analyzed by high performance liquid chromatography (HPLC). The highest amount of phenolics and tocopherols (α and β) were found at temperature of 60ºC and pressure of 30 MPa.Keywords: Supercritical carbon dioxide, Tocopherols, Totalphenolic content, Wheat bran oil
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2567187 The Effectiveness of Tebuconazole and Chitosan in Inhibiting the Growth of Fusarium Species on Winter Wheat Grain under Field Conditions
Authors: Urszula Wachowska, Anna Daria Stasiulewicz-Paluch, Katarzyna Kucharska
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A three-year field experiment (2010-2012) was conducted to determine the abundance of epiphytic and endophytic filamentous fungi colonizing the grain of winter wheat cv. Bogatka. Wheat spikes were protected with tebuconazole or chitosan at the watery ripe stage. Untreated plants served as control. Tebuconazole exerted an inhibitory effect primarily on F. culmorum and F. graminearum, and its effectiveness was determined by the pressure from pathogens that infected wheat spikes during the growing season. Chitosan did not suppress the growth of Fusarium species and Alternaria alternata.
Keywords: Winter wheat, tebuconazole, chitosan, Fusarium.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2349186 Identification and Classification of Gliadin Genes in Iranian Diploid Wheat
Authors: Jafar Ahmadi, Alireza Pour-Aboughadareh
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Wheat is the first and the most important grain of the world and its bakery property is due to glutenin and gliadin qualities. Wheat seed proteins were divided into four groups according to solubility including albumin, globulin, glutenin and prolamin or gliadin. Gliadins are major components of the storage proteins in wheat endosperm. It seems that little information is available about gliadin genes in Iranian wild relatives of wheat. Thus, the aim of this study was the evaluation of the wheat wild relatives collected from different origins of Zagros Mountains in Iran, in terms of coding gliadin genes using specific primers. For this, forty accessions of Triticum boeoticum and Triticum urartu were selected for this study. For each accession, genomic DNA was extracted and PCRs were performed in total volumes of 15 μl. The amplification products were separated on 1.5% agarose gels. In results, for Gli-2A locus three allelic variants were detected by Gli-2As primer pairs. The sizes of PCR products for these alleles were 210, 490 and 700 bp. Only five (13%) and two accessions (5%) produced 700 and 490 bp fragments when their DNA was amplified with the Gli.As.2 primer pairs. However, 93% of the accessions carried allele 210 bp, and only 8% did not any product for this marker. Therefore, these germplasm could be used as rich gene pool to broaden the genetic base of bread wheat.Keywords: Diploied wheat, gliadin, Triticum boeoticum, Triticum urartu.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1951185 RAPD Analysis of Genetic Diversity of Castor Bean
Authors: M. Vivodík, Ž. Balážová, Z. Gálová
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The aim of this work was to detect genetic variability among the set of 40 castor genotypes using 8 RAPD markers. Amplification of genomic DNA of 40 genotypes, using RAPD analysis, yielded in 66 fragments, with an average of 8.25 polymorphic fragments per primer. Number of amplified fragments ranged from 3 to 13, with the size of amplicons ranging from 100 to 1200 bp. Values of the polymorphic information content (PIC) value ranged from 0.556 to 0.895 with an average of 0.784 and diversity index (DI) value ranged from 0.621 to 0.896 with an average of 0.798. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared and analyzed genotypes were grouped into two main clusters and only two genotypes could not be distinguished. Knowledge on the genetic diversity of castor can be used for future breeding programs for increased oil production for industrial uses.
Keywords: Dendrogram, polymorphism, RAPD technique, Ricinus communis L.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2631184 Production of Cellulases by Aspergillus Heteromorphus from Wheat Straw under Submerged Fermentation
Authors: Anita Singh, Namita Singh, Narsi R. Bishnoi
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To investigate the production of cellulases from Aspergillus heteromorphus, submerged fermentation was performed using wheat straw as substrate. Optimization of saccharification conditions like pH, temperature and time were studied. Highest reducing sugar was released on 5th day at 5 pH, 30° C temperature. When A. heteromorphous was grown on wheat straw in submerged fermentation after 5 days incubation at 30 ° C, 3.2 IU/ml and 83 IU/ml, filter paper activity and CMCase activity respectively.
Keywords: Aspergillus heteromorphus, Wheat Straw, Submerged Fermentation, Production of Cellulases
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2370183 Performance of Ripped and Unripped Plantain-Wheat Flour Blend in Biscuit production
Authors: Idoko J. O., Nwajiaku I.
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Unripe and ripe plantain were dried and milled into flour and used with wheat flour in biscuit production to determine the best plantain-wheat composite flour for biscuit production. The blends as follows: 100% wheat flour, 100% ripe plantain flour, 100% unripe plantain flour, 50% wheat flour and 50% ripe plantain flour and 50% wheat flour and 50% unripe plantain flour. The Biscuit samples were stored at ambient temperature for 8 weeks after which the equilibrium moisture content and water activity were determined. The sensory evaluation of the biscuit samples was also determined. The results of these analyses showed 100% unripe plantain flour as the most stable of the BISCUIT samples judging from its equilibrium moisture content level of 0.32% and water activity of 0.62. The sensory evaluation results showed Biscuit made from 150:50 ripe plantain and wheat flour as most generally accepted at 5% level of significance.
Keywords: Biscuit, equilibrium moisture content, performance, plantain, water activity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3898182 Evaluation of Baking Properties and Sensory Quality of Wheat-Cowpea Flour
Authors: Mohamed A. Ahmed, Lydia J. Campbell
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The fortified of soft wheat flour with cowpea flour in bread making was investigated. The Soft wheat flour (SWF) was substituted by cowpea flour at levels of 5, 15 and 20%. The protein content of composite breads ranged from 6.1 – 9.9%. Significant difference was observed in moisture, protein and crude fibre contents of control (wheat bread) and composite bread at 5% addition of cowpea. Water absorption capacities of composite flours increased with increasing levels of cowpea flour in the blend. The specific loaf volume decreased significantly with increased cowpea content of blends. The overall acceptability of the 5% cowpea flour content of composite bread was not significantly different from the control (Soft Wheat-bread) but there is significantly different with increasing the levels of cowpea flour in the blend more than 5%.Keywords: Cowpea flour, wheat flour, baking properties, sensory quality.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2202181 Influence of Nitrogen Fertilization on the Yields and Grain Quality of Winter Wheat under Different Environmental Conditions
Authors: Alicja Sułek, Grażyna Cacak-Pietrzak, Marta Wyzińska, Anna Nieróbca
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In 2013/2014 and 2014/2015, a field experiment was conducted in two locations: Osiny and Wielichowo (Poland). The two-factor experiment was based on the method of randomized subblocks, in three replications. The first factor (A) was dose of nitrogen fertilization (two levels). The second factor (B) was nine winter wheat cultivars. It was found that winter wheat cultivars exhibited different reactions to higher nitrogen fertilization depending on the years and localities. Only KWS Dacanto cultivar under all growing conditions showed a significant increase in grain yield after the application of a higher level of nitrogen fertilization. The increase in nitrogen fertilization influenced the increase in gluten proteins content in wheat grain, but these changes were statistically significant only in the first year of the study. The quality of gluten does not depend on nitrogen fertilization. The quality of wheat grain depends on cultivars.
Keywords: Fertilization, grain quality, winter wheat, yield.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 903180 Evaluation of Potential Production of Maize Genotypes of Early Maturity in Rainfed Lowland
Authors: St. Subaedah, A. Takdir, Netty, D. Hidrawati
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Maize development at the rainfed lowland after rice is often confronted with the occurrence of drought stress at the time of entering the generative phase, which will cause be hampered crop production. Consequently, in the utilization of the rainfed lowland areas optimally, an effort that can be done using the varieties of early maturity to minimize crop failures due to its short rainy season. The aim of this research was evaluating the potential yield of genotypes of candidates of maize early maturity in the rainfed lowland areas. The study was conducted during May to August 2016 at South Sulawesi, Indonesia. The study used randomized block design to compare 12 treatments and consists of 8 genotypes namely CH1, CH2, CH3, CH4, CH5, CH6, CH7, CH8 and the use of four varieties, namely Bima 3, Bima 7, Lamuru and Gumarang. The results showed that genotype of CH2, CH3, CH5, CH 6, CH7 and CH8 harvesting has less than 90 days. There are two genotypes namely genotypes of CH7 and CH8 that have a fairly high production respectively of 7.16 tons / ha and 8.11 tons/ ha and significantly not different from the superior varieties Bima3.Keywords: Evaluation, maize, early maturity, yield potential.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1290179 Precipitation Change and its Implication in the Change of Winter Wheat drought and Production in North China Region from 2000 to 2010
Authors: Y. Huang, Q. J. Tian, L. T. Du, J. Liu, S. S. Li
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Understanding how precipitation inter-annually changes and its implication in agricultural drought and production change in winter wheat (Triticum aestivum L.) growth season is critical for crop production in China. MODIS Temperature-Vegetation Dryness Index (TVDI) and daily mean precipitation time series for the main growth season(Feb. to May) of winter wheat from 2000 to 2010 were used to analyze the distribution of trends of precipitation, agricultural drought and winter wheat yield change respectively, and relationships between them in North China region(Huang-huai-hai region, HHH region), China. The results indicated that the trend of precipitation in HHH region past 11 years was increasing, which had induced generally corresponding decreasing trend of agricultural drought and increasing trend of wheat yield, while the trend of drought was spatially diverse. The study could provide a basis for agricultural drought research during winter wheat season in HHH region under the ground of climate change.Keywords: drought, MODIS, precipitation change, TVDI, winter wheat production
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1647178 Development and Characterization of Wheat Bread with Lupin Flour
Authors: Paula M. R. Correia, Marta Gonzaga, Luis M. Batista, Luísa Beirão-Costa, Raquel F. P. Guiné
Abstract:
The purpose of the present work was to develop an innovative food product with good textural and sensorial characteristics. The product, a new type of bread, was prepared with wheat (90%) and lupin (10%) flours, without the addition of any conservatives. Several experiences were also done to find the most appropriate proportion of lupin flour. The optimized product was characterized considering the rheological, physical-chemical and sensorial properties. The water absorption of wheat flour with 10% of lupin was higher than that of the normal wheat flours, and Wheat Ceres flour presented the lower value, with lower dough development time and high stability time. The breads presented low moisture but a considerable water activity. The density of bread decreased with the introduction of lupin flour. The breads were quite white, and during storage the colour parameters decreased. The lupin flour clearly increased the number of alveolus, but the total area increased significantly just for the Wheat Cerealis bread. The addition of lupin flour increased the hardness and chewiness of breads, but the elasticity did not vary significantly. Lupin bread was sensorially similar to wheat bread produced with WCerealis flour, and the main differences are the crust rugosity, colour and alveolus characteristics.
Keywords: Lupin flour, physical-chemical properties, sensorial analysis, wheat flour.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2545177 Wheat Bran Carbohydrates as Substrate for Bifidobacterium lactis Development
Authors: V. Radenkovs, D. Klava, K. Juhnevica
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The present study addresses problems and solutions related to new functional food production. Wheat (Triticum aestivum L) bran obtained from industrial mill company “Dobeles dzirnavieks”, was used to investigate them as raw material like nutrients for Bifidobacterium lactis Bb-12. Enzymatic hydrolysis of wheat bran starch was carried out by α-amylase from Bacillus amyloliquefaciens (Sigma Aldrich). The Viscozyme L purchased from (Sigma Aldrich) were used for reducing released sugar. Bifidibacterium lactis Bb-12 purchased from (Probio-Tec® CHR Hansen) was cultivated in enzymatically hydrolysed wheat bran mash. All procedures ensured the number of active Bifidobacterium lactis Bb-12 in the final product reached 105 CFUg-1. After enzymatic and bacterial fermentations sample were freeze dried for analysis of chemical compounds. All experiments were performed at Faculty of Food Technology of Latvia University of Agriculture in January- March 2013. The obtained results show that both types of wheat bran (enzymatically treated and non-treated) influenced the fermentative activity and number of Bifidibacterium lactis Bb-12 viable in wheat bran mash. Amount of acidity strongly increase during the wheat bran mash fermentation. The main objective of this work was to create low-energy functional enzymatically and bacterially treated food from wheat bran using enzymatic hydrolysis of carbohydrates and following cultivation of Bifidobacterium lactis Bb-12.
Keywords: Viscozyme L, α-amylase, Bifidobacterium lactis, fermented wheat bran.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2722176 Genetic Characterization of Barley Genotypes via Inter-Simple Sequence Repeat
Authors: Mustafa Yorgancılar, Emine Atalay, Necdet Akgün, Ali Topal
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In this study, polymerase chain reaction based Inter-simple sequence repeat (ISSR) from DNA fingerprinting techniques were used to investigate the genetic relationships among barley crossbreed genotypes in Turkey. It is important that selection based on the genetic base in breeding programs via ISSR, in terms of breeding time. 14 ISSR primers generated a total of 97 bands, of which 81 (83.35%) were polymorphic. The highest total resolution power (RP) value was obtained from the F2 (0.53) and M16 (0.51) primers. According to the ISSR result, the genetic similarity index changed between 0.64–095; Lane 3 with Line 6 genotypes were the closest, while Line 36 were the most distant ones. The ISSR markers were found to be promising for assessing genetic diversity in barley crossbreed genotypes.
Keywords: Barley, crossbreed, genetic similarity, ISSR.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 919175 Identification of PIP Aquaporin Genes from Wheat
Authors: Sh. A. Yousif, M. Bhave
Abstract:
There is strong evidence that water channel proteins 'aquaporins (AQPs)' are central components in plant-water relations as well as a number of other physiological parameters. We had previously reported the isolation of 24 plasma membrane intrinsic protein (PIP) type AQPs. However, the gene numbers in rice and the polyploid nature of bread wheat indicated a high probability of further genes in the latter. The present work focused on identification of further AQP isoforms in bread wheat. With the use of altered primer design, we identified five genes homologous, designated PIP1;5b, PIP2;9b, TaPIP2;2, TaPIP2;2a, TaPIP2;2b. Sequence alignments indicate PIP1;5b, PIP2;9b are likely to be homeologues of two previously reported genes while the other three are new genes and could be homeologs of each other. The results indicate further AQP diversity in wheat and the sequence data will enable physical mapping of these genes to identify their genomes as well as genetic to determine their association with any quantitative trait loci (QTLs) associated with plant-water relation such as salinity or drought tolerance.Keywords: Aquaporins, homeologues, PIP, wheat
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2036