Screening Wheat Parents of Mapping Population for Heat and Drought Tolerance, Detection of Wheat Genetic Variation

Authors: H.R. Balouchi

Abstract:

To evaluate genetic variation of wheat (Triticum aestivum) affected by heat and drought stress on eight Australian wheat genotypes that are parents of Doubled Haploid (HD) mapping populations at the vegetative stage, the water stress experiment was conducted at 65% field capacity in growth room. Heat stress experiment was conducted in the research field under irrigation over summer. Result show that water stress decreased dry shoot weight and RWC but increased osmolarity and means of Fv/Fm values in all varieties except for Krichauff. Krichauff and Kukri had the maximum RWC under drought stress. Trident variety was shown maximum WUE, osmolarity (610 mM/Kg), dry mater, quantum yield and Fv/Fm 0.815 under water stress condition. However, the recovery of quantum yield was apparent between 4 to 7 days after stress in all varieties. Nevertheless, increase in water stress after that lead to strong decrease in quantum yield. There was a genetic variation for leaf pigments content among varieties under heat stress. Heat stress decreased significantly the total chlorophyll content that measured by SPAD. Krichauff had maximum value of Anthocyanin content (2.978 A/g FW), chlorophyll a+b (2.001 mg/g FW) and chlorophyll a (1.502 mg/g FW). Maximum value of chlorophyll b (0.515 mg/g FW) and Carotenoids (0.234 mg/g FW) content belonged to Kukri. The quantum yield of all varieties decreased significantly, when the weather temperature increased from 28 ÔùªC to 36 ÔùªC during the 6 days. However, the recovery of quantum yield was apparent after 8th day in all varieties. The maximum decrease and recovery in quantum yield was observed in Krichauff. Drought and heat tolerant and moderately tolerant wheat genotypes were included Trident, Krichauff, Kukri and RAC875. Molineux, Berkut and Excalibur were clustered into most sensitive and moderately sensitive genotypes. Finally, the results show that there was a significantly genetic variation among the eight varieties that were studied under heat and water stress.

Keywords: Abiotic stress, Genetic variation, Fluorescence, Wheat genotypes.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1058455

References:

[1] A. Al-Hakimi, and P. Mannoveux, "Morpho-physiological traits related to drought tolerance in primitive wheats". In: A. B. Damania (ed.), Biodiversity and Wheat Improvement, 199-217.Academic Press, New York.1993.
[2] R.D. Allen, "Dissection of oxidative stress tolerance using transgenic plants". Plant Physiology, 107: 1049-1054, 1995.
[3] J.R. Andrews, G.J. Bredenkamp, and N.R. Baker, "Evaluation of the role of state transitions in determining the efficiency of light utilization for CO2 assimilation in leaves". Photosynthesis Research, 38: 15-26, 1993.
[4] J.L. Araus, T. Amaro, J., Voltas, H. Nakkoul, and M.M. Nachit, "Chlorophyll fluorescence as a selection criterion for grain yield in durum wheat under Mediterranean conditions". Field Crop Research, 55: 209-223, 1998.
[5] D.I. Arnon, "Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris". Plant Physiology, 24: 1-15, 1949.
[6] K. Asada, "The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons". Annual Review of Plant Physiology and Plant Molecular Biology, 50: 601-637, 1999.
[7] F. Babani, and P. Mathis, "Effect of high temperature on some wheat varieties via chlorophyll fluorescence". Vol, IV. Proceeding of the Xth International Photosynthesis congress, Montpellier, France, 797-800, 1995.
[8] T. Balakumar, V.H.B. Vincent, and K. Paliwal, "On the interaction of UV-B radiation (280-315 nm) with water stress in crop plants". Physiologia Plantarum, 87: 217-222, 1993.
[9] M. Balota, and H.K. Lichenthaler, "Red chlorophyll fluorescence as an ecophysiological method to assess the behavior of wheat genotypes under drought and heat". Cereal Research Communication, 27: 179-187, 1999.
[10] A. Blum, "Crop responses to drought and the interpretation of adaptation". Plant Growth Regul, 20: 57-70. 1996.
[11] A. Blum, N. Klueva, and H.T. Nguyen, "Wheat cellular thermotolerance is related to yield under stress". Euphytica, 117: 117-123, 2001.
[12] G. Cornic, and A. Masacci, "Leaf photosynthesis under drought stress. In: Baker NR (ed) Photosynthesis and the Environment". Kluwer Academic Publishers, pp 347-366, 1996.
[13] B. Ehdaie, A.E. Hall, G.D. Farquhar, H.T. Nguyen, and J.G. Waines, "Water use efficiency and carbon isotope discrimination in wheat". Crop science, 31: 1282-1288, 1991.
[14] Z. Flagella, R.G. Campanile, G. Rogna, M.C. Stoppelli, D. Pastore, Ade. Caro, D-di. Fonza, A. De-Caro, and N. Di-Fonzo, "The maintenance of photosynthetic electron transport in relation to osmotic adjustment in durum wheat cultivars differing in drought resistance". Plant Science Lamerick, 118: 127-133, 1996.
[15] C.H. Foyer, M. Lelandais, and K.J. Kunert, "Photooxidative stress in plants". Physiologia Plantarum, 92: 696-717, 1994.
[16] H.A. Frank, and R.J. Cogdell, "Carotenoids in photosynthesis". Photochem Photobiol, 63: 257-264, 1996.
[17] Y.T. Gan, P.R. Millerb, B.G. McConkeya, R.P. Zentnera, F.C. Stevensonc and C.L. McDonalda, "Influence of Diverse Cropping Sequences on Durum Wheat Yield and Protein in the Semiarid Northern Great Plains". Agronomy Journal, 95: 245-252, 2003.
[18] Genstat 6 Committee. "Genstat 6 Release 3 Reference Manual". Oxford: Clarendon Press, 1997.
[19] B. Genty, J.M. Briantais, and N.R. Baker, "The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence". Biochim Biophys Acta, 990: 87-92, 1989.
[20] A.D. Hanson, and W.D. Hitz, "Metabolic responses of mesophytes to plant water deficits". Annual Review of Plant Physiology, 33: 163-203, 1983.
[21] M. Havaux, "Characterization of thermal damage to the photosynthetic electron transport system in potato leaves". Plant Science, 94: 19-33, 1992.
[22] M. Havaux, and F. Tardy, "Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landrace to high light and heat stress". Australian Journal of Plant Physiology, 26: 569- 578, 1999.
[23] J.X. He, J. Wang, and H.G. Liang, "Effects of water stress on photochemical function and protein metabolism of Photosystem II in wheat leaves". Physiology Plant, 93: 771-777. 1995.
[24] S.S. Hong, and D.Q. Xu, "Light induced increase in initial chlorophyll fluorescence F0 level and the revisable inactivation of PSII reaction centers in soybean leaves". Photosynthesis Research, 61: 269-280, 1999.
[25] I. James, L. Mprison, and R.M. Gifford, "Plant growth and water use with limited water supply in high CO2 concentrations. Leaf area, water use and transpiration". Journal of plant physiology, 11: 361-374, 1984.
[26] X.L. Jia, J.L. Jian, R.K. Ma, and J.L. Lu, "A study on water use efficiency and its components in high yielding winter wheat". Acta Agronomica Sinica, 25: 309-314, 1999.
[27] P.D. Jones, and R.S. Bradley, "Climatic variations over last 500 years. In, climate since A.D. 1500". (Eds; R.S. Bradely and P.D. Jones). pp, 665. Publisher, Loutledge, London, 1992.
[28] M.A. Karim, Y. Fracheboud, and P. Stamp, "Photosynthetic activity of developing leaves of Zea mays is less affected by heat stress than that of developed leaves". Physiologia Plantarum, 105: 685-693, 1999.
[29] M.I. Kicheva, T.D. Tsonev, and L.P. Popova, "Stomatal and nonstomatal limitations to photosynthesis in two wheat cultivars subjected to water stress". Photosynthetica, 30: 107-116, 1994.
[30] T.E. Kraus, B.D. McKersie, and R.A. Fletcher, "Paclobutrazol induced tolerance of wheat leaves to paraquat may involve increased antioxidant enzyme activity". Journal of Plant Physiology, 145: 570-576, 1995.
[31] H.K. Lichtenthaler, and A.R. wellburn, "Determination of total Carotenoids and chlorophyll a and b of leaf extracts in different solvents". Biochem. Soc. Trans, 11: 591-592, 1983.
[32] C.M. Lu, and J.H. Zhang, "Heat induced multiple effects on PS II in wheat plants". Journal of Plant Physiology, 156: 259-265, 2000.
[33] M. Menconi, C.L.M. Sgherri, C. Pinzino, and F. Navari-Izzo, "Activated oxygen production and detoxification in wheat plants subjected to a water deficit programme". J. Exp. Bot, 46: 1123-1130, 1995.
[34] J.F. Moran, M. Becana, I. Iturbe-Ormaetxe, S. Frechilla, R.V. Klucas, and P. "Aparicio-Trejo, Drought induces oxidative stress in pea plants". Planta, 194: 346-352, 1994.
[35] J.E. Muller, and M.S. Whitsitt, "Plant cellular responses to water deficit". Plant Growth Regul, 20: 41-46, 1996.
[36] J.M. Nyachiro, K.G. Briggs, J. Hoddinott, A.M. Johnson-Flanagan, "Chlorophyll content, chlorophyll fluorescence and water deficit in spring wheat". Cereal Res. Commun, 29: 135-142, 2001.
[37] A.H. Price, and O.A.F. Hendry, "Iron-catalysed oxygen radical formation and its possible contribution to drought in nine native grasses and three cereals". Plant, Cell and Environment, 14: 477-484, 1991.
[38] R.A. Richards, G.J. Rebetzke, and A.G. Condon, "Genetic improvement of water use efficiency and yield of dry land wheat". In, Proceedings 9th International Wheat Genetics Symposium, Saskatoon, Canada, Volume 1, pp, 57-60, 1998.
[39] M.M. Saadalla, "Water use efficiency and its components of wheat genotypes for varying droght tolerance". Annals of Agriculture Science Cairo. 46: 85-102, 2001.
[40] R.K. Sairam and D.C. Saxena, "Oxidative Stress and Antioxidants in Wheat Genotypes: Possible Mechanism of Water Stress Tolerance". Journal of Agronomy and Crop Science, 184: 55-61, 2000.
[41] R.K. Sairam, P.S. Deshmukh, D.S. Shukla, and S. Ram, "Metabolic activity and grain yield under moisture stress in wheat genotypes". Indian Journal of Plant Physiology, 33: 226-231, 1997.
[42] R.K. Sairam, D.S. Shukla, and D.C. Saxena, "Stress induced injury and antioxidant enzymes in relation to drought tolerance in wheat genotypes". Biol. Plant, 40: 357-364, 1997/98.
[43] C.L.M. Sgherri, and F. Navari-Izzo, "Sunflower seedlings subjected to increasing water deficit stress: Oxidative stress and defense mechanisms". Physiology Plant, 93: 25-30, 1995.
[44] C.L.M. Sgherri, C. Pinzino, and F. Navari-Izzo, "Chemical changes and O2 _ production in thylakoid membranes under water stress". Physiology Plant, 87: 211-216, 1993.
[45] C.L.M. Sgherri, C. Pinzino, and F. Navari-Izzo, "Sunflower seedlings subjected to increasing water stress by water deficit: Changes in O2 _ production related to the composition of thylakoid membranes". Physiology Plant, 96: 446-452, 1996.
[46] Z. Shangguan, M.G. Shao, and J. Dyckmans, "Effects of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat". J. Plant Physiol, 156: 46-51, 2000.
[47] T.D. Sharkey, M.R. Badger, S. Caemmerer, and T.J. Andrews, "Increased heat sensitivity of photosynthesis in tobacco plants with reduced Rubisco activase". Photosynthesis Research, 67: 147-156, 2001.
[48] N. Smirnoff, "The role of active oxygen in the response of plants to water deficit and desiccation". New Phytol, 125: 27-58, 1993.
[49] E.A. Tambussi, C.G. Bartoli, J. Beltrano, J.J. Guimet, and J.L. Araus, "Oxidative damage to thylakoid proteins in winter stressed leaves of wheat". Physiologia Plantarum, 108: 398-404, 2000.
[50] F. Tardy, A. Creach, and M. Havaux, "Photosynthetic pigment concentration. Organization and enter conversions in a pale green Syrian landrace of barley adapted to harsh climatic conditions". Plant Cell Environment, 21: 479-489, 1998.
[51] B. Vani, S.P. Pardha, and P.C. Mohanty, "Characterization of high temperature induced stress impairments in thylakoids of rice seedlings". Indian Journal of Biochemistry Biophysics, 38: 220-229, 2001.
[52] J.G. Waines, "High temperature stress in wild wheats and spring wheats". Australian Journal of plant physiology, 21: 705-715, 1994.
[53] C.C. Xu, H.Y. Lee, and C.H. Lee, "Recovery from low temperature photo inhibition is not governed by changes in the level of zeaxanthin in rice (Oryza sativa L.) leaves". Journal of Plant Physiology, 155: 755- 761, 1999.
[54] X.L. Xu, Z.M. Wang, and J.P. Zhang, "Effect of heat stress on photosynthetic characteristics of different green organs of winter wheat during grain-filling stage". Acta Bot. Sci., 43: 571-577, 2001.
[55] Z.M. Yang, S.J. Zheng, A.T. Hu, Y.F. Zheng, and J.Y. Yan, "Response of cucumber plants to increase UV-B radiation under water stress". Journal of Environmental Sciences, 12: 236-240, 2000.
[56] I. Yordanov, K. Georgieva, V. Velikova, T. Tsonev, M. Merakchiiska- Nikolova, S. Paunova, and D. Stefanov, "Response of the photosynthetic apparatus of different wheat genotypes to drought: I. Laboratory experiments under controlled light and temperature conditions". Dokl. Bolg. Akad. Nauk, 54: 79-84, 2001.
[57] A.J. Young, "The photoprotective role of carotenoids in higher plants". Physiologia Plantarum, 83: 702-708, 1991.
[58] M. Zaharieva, E. Gaulin., M. Havaux, E. Acevedo, and P. Monneveux, "Drought and heat responses in the wild wheat relative Aegilops Roth: potential interest wheat improvement". Crop Science, 41: 1321-1329, 2001.
[59] J. Zhang, and M.B. Kirkham, "Antioxidant responses to drought in sunflower and sorghum seedlings". New Phytol, 132: 361-373, 1996.