Genetic Characterization of Barley Genotypes via Inter-Simple Sequence Repeat
In this study, polymerase chain reaction based Inter-simple sequence repeat (ISSR) from DNA fingerprinting techniques were used to investigate the genetic relationships among barley crossbreed genotypes in Turkey. It is important that selection based on the genetic base in breeding programs via ISSR, in terms of breeding time. 14 ISSR primers generated a total of 97 bands, of which 81 (83.35%) were polymorphic. The highest total resolution power (RP) value was obtained from the F2 (0.53) and M16 (0.51) primers. According to the ISSR result, the genetic similarity index changed between 0.64–095; Lane 3 with Line 6 genotypes were the closest, while Line 36 were the most distant ones. The ISSR markers were found to be promising for assessing genetic diversity in barley crossbreed genotypes.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1340268Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 445
 S. A. Akladious, and S. M. Abbas, Inter-Simple Sequence Repeat (ISSR) Markers and Some Physiological Attributes of Barley (Hordeum vulgare L.) Genotypes to Drought and Potassium Nutrition. Journal of Animal And Plant Sciences Volume: 24 Issue: 2, 2014, pp. 620-633.
 M. E. Fernandez, A. M. Figueiras, and C. Benito, The Use of ISSR and RAPD Markers for Detecting DNA Polymorphisms, Genotype Identification and Genetic Diversity Among Barley Cultivars with Known Origin. Theor. Apple. Genet. Volume: 104, 2002, pp. 845-851.
 V. Giancarla, M. Emilian, and S. Radu, The use of RAPD and ISSR markers for genetic diversity among some barley cultivars. Romanian Biotechnological Letters Volume: 17, Issue: 4, 2012, pp. 7493-7503.
 C. Grativol, C. F. Lira-Medeiros, A. S. Hemerly, and P. C. Gomes Ferreira, High efficiency and reliability of inter-simple sequence repeats (ISSR) markers for evaluation of genetic diversity in Brazilian cultivated Jatropha curcas L. accessions. Mol Biol Rep., Volume: 38, Issue: 7, 2011, pp. 4245-4256.
 M. J. Hayden, and T. Tabone, D. E. Mather, Development and assessment of simple PCR markers for SNP genotyping in barley. Theor Appl Genet Volume: 119, 2009, pp. 939–951.
 P. Jaccard, Etude de la distribution florale dans une portion des Alpes et du jura. Bull Soc Vandoise Sci Nat Volume: 37, 1901, pp. 547–579.
 K. Karim, A. Rawda, C.M. Hatem, B.N. Mubarek, and T. Mokhtar, Analysis of Genetic diversity and reltionships in local Tunisian barley by RAPD and SSR analysis. African Journal of Biotechnology, Volume: 9 Issue: 44, 2010, pp. 7429-7436.
 I. G. Mylonas, A. Georgiadis, A. P. Apostolidis, K. Bladenopoulos, and M. Koutsika-Sotiriou, Barley cultivar discrimination and hybrid purity control using RAPD markers. Romanian Biotechnological Letters, Volume: 19, Issue: 3, 2014, pp. 9421-9428.
 A. Prevost, and M. J. Wilkinson, A new system of comparing PCR primers applied to ISSR fingerprinting of potato accessions. Theor Appl. Genet., Volume: 98, 1999, pp. 107–112.
 W. Powell, M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey, and A. Rafalski, The Comparison of RFLP, RAPD, AFLP and SSR (microsatellite) Markers for Germplasm Analysis. Molecular Breeding, Volume: 2, 1996, pp. 225-238.
 J. F. Rohlf, NTSYS-pc:2.2, Numerical Taxonomy and Multivariate Analysis System. (Exeter Software, Setauket, N.Y.), 2004.
 J. S. C. Smith, E. C. L. Chin, H. Shu, O. S. Smith, S. J. Wall, and M. L. Senior, An evaluation of the utility of SSR loci as molecular marker in maize (Zea mays): comparisons with data from RFLP and pedigree. Theor Appl Genet. Volume: 95, 1997, pp. 163–173.
 B. Tanyolaç, Inter-Simple Sequnce Repeat (ISSR) and RAPD Variation Among Wild Barley (Hordeum. vulgare subsp. spontaneum) Populations from West Turkey. Genetic Resources and Crop Evolution, Volume: 50, 2003, pp. 611-614.
 F. A. Tonk, M. Tosun, E. İlker, D. İstipliler, O. Tarar, Evaluation and Comparison of ISSR and RAPD Markers for Assessment of Genetic Diversity in Triticale Genotypes. Bulgarian Journal of Agricultural Science, Volume: 20, Issue: 6, 2014, pp. 1413-1420.
 A. Wang, Z. Yu, Y. Ding, Genetic diversity analysis of wild close relatives of barley from Tibet and the Middle East by ISSR and SSR markers, C. R. Biologies Volume: 332, 2009, pp. 393–403.
 A. Ciulca, S. Ciulca, E. Madoşa, S., Mihacea, C. Petolescu, RAPD analysis of genetic variation among some winter barley cultivars, Romanian Biotechnological Letters Volume: 15, No: 1, Supplement, 2010, pp. 19-24.
 M.A.H.M. El-Awady, A.A.E.S. El-Tarras, S.E.D. El-Assal, Genetic Diversity of Some Saudi Barley (Hordeum Vulgare L.) Landraces Based on Two Types of Molecular Markers. American Journal of Applied Sciences, Vol 9, Iss 5, 2012, pp. 752-758.
 O. Sofalian and M. Behi, Assessment of winter survival in barley (Hordeum vulgare L.) genotypes using molecular markers and some physiological traits. Journal of Stress Physiology & Biochemistry, Vol 9, Iss 3, 2013, pp 169-180.