Production of Cellulases by Aspergillus Heteromorphus from Wheat Straw under Submerged Fermentation
To investigate the production of cellulases from Aspergillus heteromorphus, submerged fermentation was performed using wheat straw as substrate. Optimization of saccharification conditions like pH, temperature and time were studied. Highest reducing sugar was released on 5th day at 5 pH, 30° C temperature. When A. heteromorphous was grown on wheat straw in submerged fermentation after 5 days incubation at 30 ° C, 3.2 IU/ml and 83 IU/ml, filter paper activity and CMCase activity respectively.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1055807Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF
 Singh, R., Singh, N., Parkash, A., Poonia, S., 2006. Saccharification studies by a thermophilic fungus Sporotricum thermophile isolated from agriculture waste of Bhopal, Indian Journal of Environment & Ecoplanning, 12 (1) 97-104
 Kuhad RC, Singh A, Eriksson KE (1997). Microorganisms enzymes involved in the degradation of plant fiber cell walls. Adv. Biochem. Eng. Biotechnol. 57: 45-125
 Reith, J. H., den Uil, H., van Veen, H., de Laat WTAM, Niessen, J. J. & de Jong, E. (2002). Coproduction of bioethanol, electricity and heat from biomass residues. 12th European Conference and Technology Exhibition on Biomass from Energy, Industry and Climate Protection, Amsterdam, The Netherlands, 17-21 June.
 Wen, Z., Liao, W., & Chen, S. (2005). Production of cellulase/bglucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure. Process Biochemistry, 40, 3087- 3094.
 Dale BE (1999). Biobased industrial products: bioprocess engineering when cost really counts. Biotechnol. Prog. 15: 775-776.
 Lynd LR, Wyman CE, Gerngross TU (1999). Biocommodity engineering. Biotechnol. Program. 15: 777-793.
 Gubitz, G.M., Mansfield, S.D., Bohm, D., Saddler, J.N., 1998. Effect of endoglucanases and hemicellulases in magnetic and flotation deinking of xerographic and laser-printed papers. J. Biotechnol. 65, 209-215.
 Bajpai, P., 1999. Application of enzymes in the pulp and paper industry. Biotechnol. Prog. 15, 147-157.
 Sternberg D., 1976, Production of Cellulase by Trichoderma. Biotech Bioeng. Symp. (6), 35-53.
 Miller GL (1959) Use of dinitrosalicyclic acid reagent for determination of reducing sugar. Analytical Chemistry 31: 426-428
 Ghose, T. K. (1987). Measurement of cellulase activities. Pure & Applied Chemistry 59: 257-268
 Baig MMV, Baig MLB, Baig MIA & Yasmeen M (2004) Saccharification of banana agro-waste by cellulolytic enzymes. African Journal of Biotechnology 3 (9): 447-450
 Khan MMH, Ali S, Razi AF & Alzam MZ (2007) Use of fungi for the bioconversion of rice straw into cellulose enzyme. Journal of Environmental Science and Health Part B. 42: 381-386.
 Narasimha G, Sridevi A, Viswanath, B., Chandra M. S., Reddy, R.B. 2006 Nutrient effects on production of cellulolytic enzymes by Aspergillus niger. African Journal of Biotechnology 5 (5), 472-476
 Muthuvelayudham, R and Viruthagir, 2006 Fermentative production and kinetics of cellulose protein on Tricoderma reesei ysing sugarcane bagasse and rice straw. African Journal of Biotechnology Vol. 5 (20), pp. 1873-1881, 16 october 2006
 Kang, S.W., Park, Y.S., Lee, J.S. , Hong , S.I., Kim, S.W., 2004. Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass, Bioresource Technology, 91, 153-156
 Pandey A, Selvakumar P, Soccol CR & Nigam P (1999) Solid State Cultivation for the Production of Industrial Enzymes. Current Sci. 77: 149-162
 Ojumu TV, Solomon B O, Betiku E, Layokun SK & Amigun B (2003) Cellulase Production by Aspergillus flavus Linn Isolate NSPR 101 fermented in sawdust, bagasse and corncob. African Journal of Biotechnology 2 (6): 150-152.
 Alam MZ, Muhammad N & Mahmat ME (2005) Production of cellulase from oil palm biomass as substrate by solid state bioconversion. American Journal of Applied Science 2(2):569-572
 Hattaka AI (1983) Biological pretreatment of lignocellulose. Applied Microbiol. Biotechnol. 18:350-357.
 Considine PJ, O-Rorke A, Hackett TJ & Coughlan MP (1988) Hydrolysis of beet pulp polysaccharides by extracts if solid state cultures of Penicillium capsulatum. Biotechnol. Bioeng. 31:433- 438.
 Gonzalez G, Caminal G & Lopez-Sontin (1986) Cellulase production on lignocellulose materials and performance of a packed-red reactor for enzymatic hydrolysis. Biotechnol. Bioeng. Symp. 17: 535-542.
 Okeke BC & Obi SKC (1995) Saccharification of agro-waste materials by fungal cellulases and hemicellulases. Biores. Technol. 51: 23-27.