Search results for: Mohammad Norouzi
596 Effect of Spray Stand-off on Hardness of Thermally Sprayed Coatings
Authors: M.Jalali Azizpour, S.Norouzi, H.Mohammadi Majd
Abstract:
The mechanical and tribological properties in WC-Co coatings are strongly affected by hardness and elasticity specifications. The results revealed the effect of spraying distance on microhardness and elasticity modulus of coatings. The metallurgical studies have been made on coated samples using optical microscopy, scanning electron microscopy (SEM).Keywords: HVOF, Micro-indentation, Thermal spray, WC-Co.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2330595 Bond Strength in Thermally Sprayed Gas Turbine Shafts
Authors: M.Jalali Azizpour, S.Norouzi, D.Sajedipour, H.Mohammadi majd, S.A.Hosseini, H.Talebi, A.Ghamari
Abstract:
In this paper, the bond strength of thermal spray coatings in high speed shafts has been studied. The metallurgical and mechanical studies has been made on the coated samples and shaft using optical microscopy, scanning electron microscopy (SEM).Keywords: Thermal spray, Residual stress, Wear mechanism, HVOF, Gas compressor shafts
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1794594 Study of Environmental Effects on Sunflower Oil Percent based on Graphical Method
Authors: Khodadad Mostafavi, Alireza Nabipour, Mohammad Norouzi
Abstract:
Biplot can be used to evaluate cultivars for their oil percent potential and stability and to evaluate trial sites for their discriminating ability and representativeness. Multi-environmental trial (MET) data for oil percent of 10 open pollinating sunflower cultivars were analyzed to investigate the genotype-environment interactions. The genotypes were evaluated in four locations with different climatic conditions in Iran in 2010. In each location, a Randomized Complete Block design with four replications was used. According to both mean and stability, Zaria, Master and R453, had highest performances among all cultivars. The graphical analysis identified best cultivar for each environment. Cultivars Berezans and Record performed best in Khoy and Islamabad. Zaria and R453 were the best genotypes in Sari and Karaj followed by Master and Favorit. The GGE bi-plot indicated two mega-environments, group one contained Karaj, Khoy and Islamabad and the second group contained Sari. The best discriminating location was Karaj followed with Khoy, Islamabad and Sari. The best representative genotypes were Zaria, R453, Master and Favorit. Ranking of ten cultivars based their oil percent was as Zaria > R453 ≈ Master ≈ Favorit > Record ≈ Berezans > Sor > Lakumka > Bulg3 > Bulg5.Keywords: Stability, Bi-plot, Genotype- environment interaction, Sunflower
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1392593 Effect of Spray Stand-off on Elasticity Modulus of Thermally Sprayed Coatings
Authors: M.Jalali Azizpour, S.Norouzi, H.mohammadi Majd, M.M.Rabieh, D.Sajedipour, A. Jaderi
Abstract:
The mechanical and tribological properties in WC-Co coatings are strongly affected by hardness and elasticity specifications. The results revealed the effect of spraying distance on microhardness and elasticity modulus of coatings. The metallurgical studies have been made on coated samples using optical microscopy, scanning electron microscopy (SEM).Keywords: Elasticity modulus, HVOF, Micro-indentation, Thermal spray, WC-Co
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1693592 Development Trend in Investigation of Residual Stresses in WC-Co Coating by HVOF Thermal Spraying
Authors: M.Jalali Azizpour, S.Norouzi, , H.Mohammadi Majd, D.Sajedipour , R.Mohammadi Sadr, M.Derakhshan Mehr, S.A Shoabi, R.Mohammadi
Abstract:
In this paper, the techniques for estimating the residual stress in high velocity oxy fuel thermal spray coatings have been discussed and compared. The development trend and the last investigation have been studied. It is seemed that the there is not effective study on the effect of the peening action in HVOF analytically and numerically.Keywords: HVOF, WC-Co, Residual stress, Compressive stress
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2388591 Residual Stresses in Thermally Sprayed Gas Turbine Components
Authors: M.Jalali Azizpour, S.Norouzi, D.Sajedipour, H.Mohammadi Majd
Abstract:
In this paper, the residual stress of thermal spray coatings in gas turbine component by curvature method has been studied. The samples and shaft were coated by hard WC-12Co cermets using high velocity oxy fuel (HVOF) after preparation in same conditions. The curvature of coated samples was measured by using of coordinate measurement machine (CMM). The metallurgical and Tribological studies has been made on the coated shaft using optical microscopy and scanning electron microscopy (SEM)Keywords: Thermal spray, Residual stress, Wear mechanism, HVOF, Gas compressor shafts
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1690590 Advances on LuGre Friction Model
Authors: Mohammad Fuad Mohammad Naser, Faycal Ikhouane
Abstract:
LuGre friction model is an ordinary differential equation that is widely used in describing the friction phenomenon for mechanical systems. The importance of this model comes from the fact that it captures most of the friction behavior that has been observed including hysteresis. In this paper, we study some aspects related to the hysteresis behavior induced by the LuGre friction model.
Keywords: Hysteresis, LuGre model, operator, (strong) consistency.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2709589 Adaptive Car Safety System
Authors: Shahram Jafari, Mohammad-Ali Nikouei Mahani, Mohammad Arabnezhad, Mahdi Sharifi
Abstract:
Car accident is one of the major causes of death in many countries. Many researchers have attempted to design and develop techniques to increase car safety in the past recent years. In spite of all the efforts, it is still challenging to design a system adaptive to the driver rather than the automotive characteristics. In this paper, the adaptive car safety system is explained which attempts to find a balance.
Keywords: Analog to Digital Converter (ADC), AdaptiveCar Safety System, Multi-Media Card (MMC).
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1894588 New Exact Solutions for the (3+1)-Dimensional Breaking Soliton Equation
Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi
Abstract:
In this work, we obtain some analytic solutions for the (3+1)-dimensional breaking soliton after obtaining its Hirota-s bilinear form. Our calculations show that, three-wave method is very easy and straightforward to solve nonlinear partial differential equations.
Keywords: (3+1)-dimensional breaking soliton equation, Hirota'sbilinear form.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1670587 Some Complexiton Type Solutions of the (3+1)-Dimensional Jimbo-Miwa Equation
Authors: Mohammad Taghi Darvishi, Mohammad Najafi
Abstract:
By means of the extended homoclinic test approach (shortly EHTA) with the aid of a symbolic computation system such as Maple, some complexiton type solutions for the (3+1)-dimensional Jimbo-Miwa equation are presented.
Keywords: Jimbo-Miwa equation, painleve analysis, Hirota's bilinear form, computerized symbolic computation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1893586 Babbitt Casting and Babbitt Spraying Processes Case Study
Authors: M. Jalali Azizpour, S.Norouzi H. Mohammadi Majd
Abstract:
In this paper, the babbitting of a bearing in boiler feed pump of an electromotor has been studied. These bearings have an important role in reducing the shut down times in the pumps, compressors and turbines. The most conventional method in babbitting is casting as a melting method. The comparison between thermal spray and casting methods in babbitting shows that the thermal spraying babbitt layer has better performance and tribological behavior. The metallurgical and tribological analysis such as SEM, EDS and wet chemical analysis has been made in the Babbitt alloys and worn surfaces. Two type of babbitt materials: tinbase and lead-base babbitt was used. The benefits of thermally sprayed babbitt layers are completely clear especially in large bearings.
Keywords: Thermal spray, Babbitting, Bonding, Bearing, BFP, CWP
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 4651585 Application of HVOF Thermal Spraying inHigh Speed Gas Compressor Shafts
Authors: M.Jalali Azizpour, S.norouzi, H.mohammadi majd, H.Talebi, A.Ghamari
Abstract:
In this paper, the application of thermal spray coatings in high speed shafts by a revolution up to 23000 RPM has been studied. Gas compressor shafts are worn in contact zone with journal therefore will be undersized. Wear mechanisms of compressor shaft were identified. The predominant wear mechanism is abrasion wear. The worn surface was coated by hard WC-Co cermets using high velocity oxy fuel (HVOF) after preparation. The shafts were in satisfactory service in 8000h period. The metallurgical and Tribological studies has been made on the worn and coated shaft using optical microscopy, scanning electron microscopy (SEM) and X-ray diffraction.Keywords: Thermal spray, Residual stress, Wear mechanism, HVOF, Gas compressor shafts.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2476584 New Application of EHTA for the Generalized(2+1)-Dimensional Nonlinear Evolution Equations
Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi
Abstract:
In this paper, the generalized (2+1)-dimensional Calogero-Bogoyavlenskii-Schiff (shortly CBS) equations are investigated. We employ the Hirota-s bilinear method to obtain the bilinear form of CBS equations. Then by the idea of extended homoclinic test approach (shortly EHTA), some exact soliton solutions including breather type solutions are presented.
Keywords: EHTA, (2+1)-dimensional CBS equations, (2+1)-dimensional breaking solution equation, Hirota's bilinear form.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1487583 The Temperature Range in the Simulation of Residual Stress and Hot Tearing During Investment Casting
Authors: Saeid Norouzi, Ali Shams, Hassan Farhangi, Alireza Darvish
Abstract:
Hot tear cracking and residual stress are two different consequences of thermal stress both of which can be considered as casting problem. The purpose of the present study is simulation of the effect of casting shape characteristic on hot tearing and residual stress. This study shows that the temperature range for simulation of hot tearing and residual stress are different. In this study, in order to study the development of thermal stress and to predict the hot tearing and residual stress of shaped casting, MAGMASOFT simulation program was used. The strategy of this research was the prediction of hot tear location using pinpointing hot spot and thermal stress concentration zones. The results shows that existing of stress concentration zone increases the hot tearing probability and consequently reduces the amount of remaining residual stress in casting parts.
Keywords: Hot tearing, residual stress, simulation, investment casting.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2721582 Some Exact Solutions of the (2+1)-Dimensional Breaking Soliton Equation using the Three-wave Method
Authors: Mohammad Taghi Darvishi, Mohammad Najafi
Abstract:
This paper considers the (2+1)-dimensional breaking soliton equation in its bilinear form. Some exact solutions to this equation are explicitly derived by the idea of three-wave solution method with the assistance of Maple. We can see that the new idea is very simple and straightforward.
Keywords: Soliton solution, computerized symbolic computation, painleve analysis, (2+1)-dimensional breaking soliton equation, Hirota's bilinear form.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1906581 The Impact of e-Learning and e-Teaching
Authors: Mohammad Mohammad
Abstract:
With the exponential progress of technological development comes a strong sense that events are moving too quickly for our schools and that teachers may be losing control of them in the process. This paper examines the impact of e-learning and e-teaching in universities, from both the student and teacher perspective. In particular, it is shown that e-teachers should focus not only on the technical capacities and functions of IT materials and activities, but must attempt to more fully understand how their e-learners perceive the learning environment. From the e-learner perspective, this paper indicates that simply having IT tools available does not automatically translate into all students becoming effective learners. More evidence-based evaluative research is needed to allow e-learning and e-teaching to reach full potential.Keywords: e-learning, e-teaching, distance learning, education
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 4824580 Fuzzy Control of a Three Phase ThyristorizedInduction Motor
Authors: Abolfazl Jalilvand, Mohammad Reza Feyzi, Sohrab Khanmohammad, Mohammad Bagher Bana Sharifian, Ali Sajjadi
Abstract:
Nowadays the control of stator voltage at a constant frequency is one of the traditional and low expense methods in order to control the speed of induction motors near its nominal speed. The torque of induction motor is a nonlinear function of the firing angle, phase angle and speed. In this paper the speed control of induction motor regarding various load torque and under different conditions will be investigated based on a fuzzy controller with inverse training.
Keywords: Three phase induction motor, AC converter, speedcontrol, fuzzy control.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1795579 Labeling Method in Steganography
Authors: H. Motameni, M. Norouzi, M. Jahandar, A. Hatami
Abstract:
In this paper a way of hiding text message (Steganography) in the gray image has been presented. In this method tried to find binary value of each character of text message and then in the next stage, tried to find dark places of gray image (black) by converting the original image to binary image for labeling each object of image by considering on 8 connectivity. Then these images have been converted to RGB image in order to find dark places. Because in this way each sequence of gray color turns into RGB color and dark level of grey image is found by this way if the Gary image is very light the histogram must be changed manually to find just dark places. In the final stage each 8 pixels of dark places has been considered as a byte and binary value of each character has been put in low bit of each byte that was created manually by dark places pixels for increasing security of the main way of steganography (LSB).
Keywords: Binary image, labeling, low bit, neighborhood, RGB image, steganography, threshold.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2135578 Level Set and Morphological Operation Techniques in Application of Dental Image Segmentation
Authors: Abdolvahab Ehsani Rad, Mohd Shafry Mohd Rahim, Alireza Norouzi
Abstract:
Medical image analysis is one of the great effects of computer image processing. There are several processes to analysis the medical images which the segmentation process is one of the challenging and most important step. In this paper the segmentation method proposed in order to segment the dental radiograph images. Thresholding method has been applied to simplify the images and to morphologically open binary image technique performed to eliminate the unnecessary regions on images. Furthermore, horizontal and vertical integral projection techniques used to extract the each individual tooth from radiograph images. Segmentation process has been done by applying the level set method on each extracted images. Nevertheless, the experiments results by 90% accuracy demonstrate that proposed method achieves high accuracy and promising result.
Keywords: Integral production, level set method, morphological operation, segmentation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 4230577 Quantitative Analysis of PCA, ICA, LDA and SVM in Face Recognition
Authors: Liton Jude Rozario, Mohammad Reduanul Haque, Md. Ziarul Islam, Mohammad Shorif Uddin
Abstract:
Face recognition is a technique to automatically identify or verify individuals. It receives great attention in identification, authentication, security and many more applications. Diverse methods had been proposed for this purpose and also a lot of comparative studies were performed. However, researchers could not reach unified conclusion. In this paper, we are reporting an extensive quantitative accuracy analysis of four most widely used face recognition algorithms: Principal Component Analysis (PCA), Independent Component Analysis (ICA), Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM) using AT&T, Sheffield and Bangladeshi people face databases under diverse situations such as illumination, alignment and pose variations.
Keywords: PCA, ICA, LDA, SVM, face recognition, noise.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2430576 A Review of Control Schemes for Active Power Filters in Order to Power Quality Improvement
Authors: Mohammad Hasan Raouf, Azim Nowbakht, Mohammad Bagher Haddadi, Mohammad Reza Tabatabaei
Abstract:
Power quality has become a very important issue recently due to the impact on electricity suppliers, equipment manufacturers and customers. Power quality is described as the variation of voltage, current and frequency in a power system. Voltage magnitude is one of the major factors that determine the quality of power. Indeed, custom power technology, the low-voltage counterpart of the more widely known flexible ac transmission system (FACTS) technology, aimed at high-voltage power transmission applications, has emerged as a credible solution to solve many problems relating to power quality problems. There are various power quality problems such as voltage sags, swells, flickers, interruptions and harmonics etc. Active Power Filter (APF) is one of the custom power devices and can mitigate harmonics, reactive power and unbalanced load currents originating from load side. In this study, an extensive review of APF studies, the advantages and disadvantages of each introduced methods are presented. The study also helps the researchers to choose the optimum control techniques and power circuit configuration for APF applications.
Keywords: Power Quality, Custom Power, Active Filter, Control Approach.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3470575 Stabilization of the Lorenz Chaotic Equations by Fuzzy Controller
Authors: Behrooz Rezaie, Zahra Rahmani Cherati, Mohammad Reza Jahed Motlagh, Mohammad Farrokhi
Abstract:
In this paper, a fuzzy controller is designed for stabilization of the Lorenz chaotic equations. A simple Mamdani inference method is used for this purpose. This method is very simple and applicable for complex chaotic systems and it can be implemented easily. The stability of close loop system is investigated by the Lyapunov stabilization criterion. A Lyapunov function is introduced and the global stability is proven. Finally, the effectiveness of this method is illustrated by simulation results and it is shown that the performance of the system is improved.Keywords: Chaotic system, Fuzzy control, Lorenz equation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2028574 Comparison of the Existing Methods in Determination of the Characteristic Polynomial
Authors: Mohammad Saleh Tavazoei, Mohammad Haeri
Abstract:
This paper presents comparison among methods of determination of the characteristic polynomial coefficients. First, the resultant systems from the methods are compared based on frequency criteria such as the closed loop bandwidth, gain and phase margins. Then the step responses of the resultant systems are compared on the basis of the transient behavior criteria including overshoot, rise time, settling time and error (via IAE, ITAE, ISE and ITSE integral indices). Also relative stability of the systems is compared together. Finally the best choices in regards to the above diverse criteria are presented.Keywords: Characteristic Polynomial, Transient Response, Filters, Stability.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2012573 Exact Three-wave Solutions for High Nonlinear Form of Benjamin-Bona-Mahony-Burgers Equations
Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi
Abstract:
By means of the idea of three-wave method, we obtain some analytic solutions for high nonlinear form of Benjamin-Bona- Mahony-Burgers (shortly BBMB) equations in its bilinear form.
Keywords: Benjamin-Bona-Mahony-Burgers equations, Hirota's bilinear form, three-wave method.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1575572 Preparation and Evaluation of New Nanocatalysts for Selective Oxidation of H2S to Sulfur
Authors: Mohammad Rezaee, Mohammad Kazemeini, Ali Morad Rashidi, Moslem Fattahi
Abstract:
Selective oxidation of H2S to elemental sulfur in a fixed bed reactor over newly synthesized alumina nanocatalysts was physio-chemically investigated and results compared with a commercial Claus catalyst. Amongst these new materials, Al2O3- supported sodium oxide prepared with wet chemical technique and Al2O3 nanocatalyst prepared with spray pyrolysis method were the most active catalysts for selective oxidation of H2S to elemental sulfur. Other prepared nanocatalysts were quickly deactivated, mainly due to the interaction with H2S and conversion into sulfides.Keywords: H2S, Claus process, Al2O3, Spray pyrolysis method, Wet chemical technique
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2511571 Convective Heat Transfer of Viscoelastic Flow in a Curved Duct
Authors: M. Norouzi, M. H. Kayhani, M. R. H. Nobari, M. Karimi Demneh
Abstract:
In this paper, fully developed flow and heat transfer of viscoelastic materials in curved ducts with square cross section under constant heat flux have been investigated. Here, staggered mesh is used as computational grids and flow and heat transfer parameters have been allocated in this mesh with marker and cell method. Numerical solution of governing equations has being performed with FTCS finite difference method. Furthermore, Criminale-Eriksen- Filbey (CEF) constitutive equation has being used as viscoelastic model. CEF constitutive equation is a suitable model for studying steady shear flow of viscoelastic materials which is able to model both effects of the first and second normal stress differences. Here, it is shown that the first and second normal stresses differences have noticeable and inverse effect on secondary flows intensity and mean Nusselt number which is the main novelty of current research.Keywords: Viscoelastic, fluid flow, heat convection, CEF model, curved duct, square cross section.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2153570 Optimization of Diverter Box Configuration in a V94.2 Gas Turbine Exhaust System using Numerical Simulation
Authors: A. Mohajer, A. Noroozi, S. Norouzi
Abstract:
The bypass exhaust system of a 160 MW combined cycle has been modeled and analyzed using numerical simulation in 2D prospective. Analysis was carried out using the commercial numerical simulation software, FLUENT 6.2. All inputs were based on the technical data gathered from working conditions of a Siemens V94.2 gas turbine, installed in the Yazd power plant. This paper deals with reduction of pressure drop in bypass exhaust system using turning vanes mounted in diverter box in order to alleviate turbulent energy dissipation rate above diverter box. The geometry of such turning vanes has been optimized based on the flow pattern at diverter box inlet. The results show that the use of optimized turning vanes in diverter box can improve the flow pattern and eliminate vortices around sharp edges just before the silencer. Furthermore, this optimization could decrease the pressure drop in bypass exhaust system and leads to higher plant efficiency.
Keywords: Numerical simulation, Diverter box, Turning vanes, Exhaust system
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2802569 Flood Scenarios for Hydrological and Hydrodynamic Modelling
Authors: Md. Sharif Imam Ibne Amir, Mohammad Masud Kamal khan, Mohammad Golam Rasul, Raj H Sharma, Fatema Akram
Abstract:
Future flood can be predicted using the probable maximum flood (PMF). PMF is calculated using the historical discharge or rainfall data considering the other climatic parameters remaining stationary. However climate is changing globally and the key climatic variables are temperature, evaporation, rainfall and sea level rise are likely to change. To develop scenarios to a basin or catchment scale these important climatic variables should be considered. Nowadays scenario based on climatic variables is more suitable than PMF. Six scenarios were developed for a large Fitzroy basin and presented in this paper.
Keywords: Climate change, rainfall, potential evaporation, scenario, sea level rise (SLR), sub-catchment.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3375568 Detection of Legionella pneumophila in Cooling Water Systems of Hospitals and Nursing Homes of Kerman City, Iran by Semi- Nested PCR
Authors: Mohammad Ahmadinejad, Mohammad Reza Shakibaie, Kyvan Shams, Mohammad Khalili
Abstract:
Legionella pneumophila is involved in more than 95% cases of severe atypical pneumonia. Infection is mainly by inhalation the indoor aerosols through the water-coolant systems. Because some Legionella strains may be viable but not culturable, therefore, Taq polymerase, DNA amplification and semi-nested-PCR were carried out to detect Legionella-specific 16S-rDNA sequence. For this purpose, 1.5 litter of water samples from 77 water-coolant system were collected from four different hospitals, two nursing homes and one student hostel in Kerman city of Iran, each in a brand new plastic bottle during summer season of 2006 (from April to August). The samples were filtered in the sterile condition through the Millipore Membrane Filter. DNA was extracted from membrane and used for PCR to detect Legionella spp. The PCR product was then subjected to semi-nested PCR for detection of L. pneumophila. Out of 77 water samples that were tested by PCR, 30 (39%) were positive for most species of Legionella. However, L. pneumophila was detected from 14 (18.2%) water samples by semi-nested PCR. From the above results it can be concluded that water coolant systems of different hospitals and nursing homes in Kerman city of Iran are highly contaminated with L. pneumophila spp. and pose serious concern. So, we recommend avoiding such type of coolant system in the hospitals and nursing homes.Keywords: Legionella pneumophila, water-coolant system, semi-nested -PCR.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2055567 Synthesis of Sterile and Pyrogen Free Biogenic Magnetic Nanoparticles: Biotechnological Potential of Magnetotactic Bacteria for Production of Nanomaterials
Authors: Saeid Ghorbanzadeh-Mashkani, Parisa Tajer-Mohammad-Ghazvini, Ahmad Nozad-Golikand, Rouha Kasra-Kermanshahi, Mohammad-Reza Davarpanah
Abstract:
Today, biogenic magnetite nanoparticles among magnetic nanoparticles have unique attracted attention because of their magnetic characteristics and potential applications in various fields such as therapeutic and diagnostic. A well known example of these biogenic nanoparticles is magnetosomes of magnetotactic bacteria. In this research, we used two different types of technique for the isolation and purification of magnetosome nanoparticles from the isolated magnetotactic bacterial cells, heat-alkaline treatment and sonication. Also we evaluated pyrogen content and sterility of synthesized the isolated individual magnetosome by the Limulus Amoebocyte Lysate test and direct impedimetric method respectively.Keywords: Biogenic magnetic nanoparticles, Magnetosome, Magnetotactic bacteria, Nanobiotechnology
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2887