Search results for: Multiple site damage
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 2520

Search results for: Multiple site damage

1590 Structural Engineering Forensic Evaluation of Misdiagnosed Concrete Masonry Wall Cracking

Authors: W. C. Bracken

Abstract:

Given that concrete masonry walls are expected to experience shrinkage combined with thermal expansion and contraction, and in some cases even carbonation, throughout their service life, cracking is to be expected. However, after concrete masonry walls have been placed into service, originally anticipated and accounted for cracking is often misdiagnosed as a structural defect. Such misdiagnoses often result in or are used to support litigation. This paper begins by discussing the causes and types of anticipated cracking within concrete masonry walls followed by a discussion on the processes and analyses that exists for properly evaluating them and their significance. From here, the paper then presents a case of misdiagnosed concrete masonry cracking and the flawed logic employed to support litigation.

Keywords: Concrete masonry, masonry wall cracking, structural defect, structural damage, construction defect, forensic investigation.

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1589 Beekeeping in Libya

Authors: M. Keshlaf

Abstract:

Honey bees are the most important insects because of their ecologic and economic impacts. They pollinate more than 200 flowering crop plants resulting in an increased yield. Also, honey bees provide multiple products such as honey, royal jelly, wax, venom, pollen and propolis. Beekeeping has been practiced by Africans in all parts of the continent for many thousands of years. However, there is a little scientific information published worldwide about beekeeping in Libya. This review article aims to shed light on the history and current status of honey bee keeping in Libya.

Keywords: Apis mellifera, Libya, beekeeping.

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1588 Science School Was Burned: A Case Study of Crisis Management in Thailand

Authors: Proud Arunrangsiwed

Abstract:

This study analyzes the crisis management and image repair strategies during the crisis of Mahidol Wittayanusorn School (MWIT) library burning. The library of this school was burned by a 16-year-old-male student on June 6th, 2010. This student blamed the school that the lesson was difficult, and other students were selfish. Although no one was in the building during the fire, it had caused damage to the building, books and electronic supplies around 130 million bahts (4.4 million USD). This event aroused many discourses arguing about the education system and morality. The strategies which were used during crisis were denial, shift the blame, bolstering, minimization, and uncertainty reduction. The results of using these strategies appeared after the crisis. That was the numbers of new students, who registered for the examination to get into this school in the later years, have remained the same.

Keywords: School, crisis management, violence, image repair strategies, uncertainty, burn.

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1587 On-The-Spot Spectators- Motivations, Experiences, and Satisfactions at the 2011 TPGA Ever Rich Championship – North Bay Open

Authors: Li-Wei Liu, Cheng-Yu Tsai, Ming-Tsang Wu

Abstract:

The study investigated the 2011 TPGA Ever Rich Championship – North Bay Open spectators- on-the-site spectating motivations, experiences, and satisfactions. The research was conducted on a convenience sample of the on-the-spot spectators at the North Bay Golf and Country Club. A total of 200 questionnaires were distributed, of which 185 valid questionnaires were collected, approaching a 92.5% response rate. The data obtained was analyzed with statistical techniques. First, the data showed significant differences in motivations, experiences, and satisfactions relative to demographic variables among the on-the-spot spectators. Second, spectating motivation, experience, and satisfaction were significantly related to one another.

Keywords: Spectating motivation, spectating experience, spectating satisfaction.

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1586 CFD Simulation of Surge Wave Generated by Flow-Like Landslides

Authors: Liu-Chao Qiu

Abstract:

The damage caused by surge waves generated in water bodies by flow-like landslides can be very high in terms of human lives and economic losses. The complicated phenomena occurred in this highly unsteady process are difficult to model because three interacting phases: air, water and sediment are involved. The problem therefore is challenging since the effects of non-Newtonian fluid describing the rheology of the flow-like landslides, multi-phase flow and free surface have to be included in the simulation. In this work, the commercial computational fluid dynamics (CFD) package FLUENT is used to model the surge waves due to flow-like landslides. The comparison between the numerical results and experimental data reported in the literature confirms the accuracy of the method.

Keywords: Flow-like landslide, surge wave, VOF, non-Newtonian fluids, multi-phase flows, free surface flow.

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1585 Analysis of Supply Chain Risk Management Strategies: Case Study of Supply Chain Disruptions

Authors: Marcelo Dias Carvalho, Leticia Ishikawa

Abstract:

Supply Chain Risk Management refers to a set of strategies used by companies to avoid supply chain disruption caused by damage at production facilities, natural disasters, capacity issues, inventory problems, incorrect forecasts, and delays. Many companies use the techniques of the Toyota Production System, which in a way goes against a better management of supply chain risks. This paper studies key events in some multinationals to analyze the trade-off between the best supply chain risk management techniques and management policies designed to create lean enterprises. The result of a good balance of these actions is the reduction of losses, increased customer trust in the company and better preparedness to face the general risks of a supply chain.

Keywords: Supply chain disruptions, supply chain management, supply chain resilience, just-in-time production, lean manufacturing.

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1584 Net-Trainer-ST: A Swiss Army Knife for Pentesting, Based on Single Board Computer, for Cybersecurity Professionals and Hobbyists

Authors: K. Hołda, D. Śliwa, K. Daniec

Abstract:

This article was created as part of the developed master's thesis. It attempts to present a developed device, which will support the work of specialists dealing with broadly understood cybersecurity terms. The device is contrived to automate security tests. In addition, it simulates potential cyberattacks in the most realistic way possible, without causing permanent damage to the network, in order to maximize the quality of the subsequent corrections to the tested network systems. The proposed solution is a fully operational prototype created from commonly available electronic components and a single board computer. The focus of the article is not only put on the hardware part of the device but also on the theoretical and applicatory way in which implemented cybersecurity tests operate and examples of their results.

Keywords: Raspberry Pi, ethernet, automated cybersecurity tests, ARP, DNS, backdoor, TCP, password sniffing.

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1583 Monotonic and Cyclic J-integral Estimation for Through-Wall Cracked Straight Pipes

Authors: Rohit, S. Vishnuvardhan, P. Gandhi, Nagesh R. Iyer

Abstract:

The evaluation of energy release rate and centre Crack Opening Displacement (COD) for circumferential Through-Wall Cracked (TWC) pipes is an important issue in the assessment of critical crack length for unstable fracture. The ability to predict crack growth continues to be an important component of research for several structural materials. Crack growth predictions can aid the understanding of the useful life of a structural component and the determination of inspection intervals and criteria. In this context, studies were carried out at CSIR-SERC on Nuclear Power Plant (NPP) piping components subjected to monotonic as well as cyclic loading to assess the damage for crack growth due to low-cycle fatigue in circumferentially TWC pipes.

Keywords: 304LN stainless steel, cyclic J-integral, Elastic- Plastic Fracture Mechanics, J-integral, Through-wall crack

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1582 Ecotoxicological Studies of Soil Using Analytical and Biological Methods: A Review

Authors: V. Chahal, A. Nagpal, Y. B. Pakade, J. K. Katnoria

Abstract:

Soil is a complex physical and biological system that provides support, water, nutrients and oxygen to the plants. Apart from these, it acts as a connecting link between inorganic, organic and living components of the ecosystem. In recent years, presence of xenobiotics, alterations in the natural soil environment, application of pesticides/inorganic fertilizers, percolation of contaminated surface water as well as leachates from landfills to subsurface strata and direct discharge of industrial wastes to the land have resulted in soil pollution which in turn has posed severe threats to human health especially in terms of causing carcinogenicity by direct DNA damage. The present review is an attempt to summarize literature on sources of soil pollution, characterization of pollutants and their consequences in different living systems.

Keywords: Soil Pollution, Contaminants, Heavy metals, Pesticides, Bioassays.

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1581 Penetration Analysis for Composites Applicable to Military Vehicle Armors, Aircraft Engines and Nuclear Power Plant Structures

Authors: Dong Wook Lee

Abstract:

This paper describes a method for analyzing penetration for composite material using an explicit nonlinear Finite Element Analysis (FEA). This method may be used in the early stage of design for the protection of military vehicles, aircraft engines and nuclear power plant structures made of composite materials. This paper deals with simple ballistic penetration tests for composite materials and the FEA modeling method and results. The FEA was performed to interpret the ballistic field test phenomenon regarding the damage propagation in the structure subjected to local foreign object impact.

Keywords: Computer Aided Engineering, CAE, Finite Element Analysis, FEA, impact analysis, penetration analysis, composite material.

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1580 Sustainability Management for Wine Production: A Case of Thailand

Authors: Muthatakul Metasit, Setthasakko Watchaneeporn

Abstract:

At present, increased concerns about global environmental problems have magnified the importance of sustainability management. To move towards sustainability, companies need to look at everything from a holistic perspective in order to understand the interconnections between economic growth and environmental and social sustainability. This paper aims to gain an understanding of key determinants that drive sustainability management and barriers that hinder its development. It employs semi-structured interviews with key informants, site observation and documentation. The informants are production, marketing and environmental managers of the leading wine producer, which aims to become an Asia-s leader in wine & wine based products. It is found that corporate image and top management leadership are the primary factors influencing the adoption of sustainability management. Lack of environmental knowledge and inefficient communication are identified as barriers.

Keywords: Environmental, knowledge; Sustainability management; Top management leadership; Wine industry

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1579 Enhancement of Tribological Behavior for Diesel Engine Piston of Solid Skirt by an Optimal Choice of Interface Material

Authors: M. Amara, M. Tahar Abbes, A. Dokkiche, M. Benbrike

Abstract:

Shear stresses generate frictional forces thus lead to the reduction of engine performance due to the power losses. This friction can also cause damage to the piston material. Thus, the choice of an optimal material for the piston is necessary to improve the elastohydrodynamical contacts of the piston. In this study, to achieve this objective, an elastohydrodynamical lubrication model that satisfies the best tribological behavior of the piston with the optimum choice of material is developed. Several aluminum alloys composed of different components are studied in this simulation. An application is made on the piston 60 x 120 mm Diesel engine type F8L413 currently mounted on Deutz trucks TB230 by using different aluminum alloys where alloys based on aluminum-silicon have better tribological performance.

Keywords: EHD lubricated contacts, friction, properties of materials, tribological performance.

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1578 Cannabidiol Treatment Ameliorates Acetaminophen-Induced Hepatotoxicity in Mice

Authors: Amr A. Fouad, Waleed H. Albuali, Iyad Jresat

Abstract:

The possible therapeutic effect of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated against acute hepatotoxicity induced by a single oral dose of acetaminophen (500mg/kg) in mice. Cannabidiol (two intraperitoneal injections, 5mg/kg, each) was given 1 hour and 12 hours following acetaminophen administration. Acetaminophen administration caused significant elevations of serum alanine aminotransferase, and hepatic malondialdehyde, and nitric oxide levels, and a significant decrease in hepatic reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters resulted from acetaminophen administration. Also, histopathological examination showed that cannabidiol markedly attenuated ameliorated acetaminophen-induced liver tissue damage. These results emphasize that cannabidiol represents a potential therapeutic option to protect against acetaminophen hepartotoxicity which is a common clinical problem.

Keywords: cannabidiol, acetaminophen, liver, mice.

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1577 Seismic Fragility for Sliding Failure of Weir Structure Considering the Process of Concrete Aging

Authors: HoYoung Son, Ki Young Kim, Woo Young Jung

Abstract:

This study investigated the change of weir structure performances when durability of concrete, which is the main material of weir structure, decreased due to their aging by mean of seismic fragility analysis. In the analysis, it was assumed that the elastic modulus of concrete was reduced by 10% in order to account for their aged deterioration. Additionally, the analysis of seismic fragility was based on Monte Carlo Simulation method combined with a 2D nonlinear finite element in ABAQUS platform with the consideration of deterioration of concrete. Finally, the comparison of seismic fragility of model pre- and post-deterioration was made to study the performance of weir. Results show that the probability of failure in moderate damage for deteriorated model was found to be larger than pre-deterioration model when peak ground acceleration (PGA) passed 0.4 g.

Keywords: Weir, FEM, concrete, fragility, aging.

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1576 Condition Monitoring System of Mine Air Compressors Based on Wireless Sensor Network

Authors: Sheng Fu, Yinbo Gao, Hao Lin

Abstract:

In the current mine air compressors monitoring system, there are some difficulties in the installation and maintenance because of the wired connection. To solve the problem, this paper introduces a new air compressors monitoring system based on ZigBee in which the monitoring parameters are transmitted wirelessly. The collecting devices are designed to form a cluster network to collect vibration, temperature, and pressure of air cylinders and other parameters. All these devices are battery-powered. Besides, the monitoring software in PC is developed using MFC. Experiments show that the designed wireless sensor network works well in the site environmental condition and the system is very convenient to be installed since the wireless connection. This monitoring system will have a wide application prospect in the upgrade of the old monitoring system of the air compressors.

Keywords: Condition monitoring, wireless sensor network, air compressor, ZigBee, data collecting

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1575 The Adsorption of Lead from Aqueous Solutions Using Coal Fly Ash : Effect of Crystallinity

Authors: Widi Astuti, Agus Prasetya, Endang Tri Wahyuni, I Made Bendiyasa

Abstract:

Coal fly ash (CFA) generated by coal-based thermal power plants is mainly composed of some oxides having high crystallinity, like quartz and mullite. In this study, the effect of CFA crystallinity toward lead adsorption capacity was investigated. To get solid with various crystallinity, the solution of sodium hydroxide (NaOH) of 1-7 M was used to treat CFA at various temperature and reflux time. Furthermore, to evaluate the effect of NaOH-treated CFA with respect to adsorption capacity, the treated CFA were examine as adsorbent for removing lead in the solution. The result shows that using NaOH to treat CFA causes crystallinity of quartz and mullite decrease. At higher NaOH concentration (>3M), in addition the damage of quartz and mullite crystallinity is followed by crystal formation called hydroxysodalite. The lower crystalllinity, the higher adsorption capacity.

Keywords: Coal fly ash, crystallinity, lead, adsorption capacity

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1574 Effects of Modified Bottom Boards on the Performance of Honeybee Colonies

Authors: M. Keshlaf, R. Spooner-Hart

Abstract:

Australia does not have varroa mite. However, we investigated whether modified hive bottom boards used for varroa mite management in honey bee colonies had other benefits, for honey production. We compared a number of colony parameters between hives fitted with tube, mesh and conventional (solid) bottom boards in two locations in eastern Australian, Richmond NSW and Castlemaine Victoria. Colonies housed in hives with mesh and tube bottom boards were not significantly superior to those in hives with conventional bottom boards with regard to bee flight activity, nor did they produce more honey, brood or stored pollen, in either experimental site. Although the trial was conducted over only one season, it is suggested that there may be no benefit in Australian bee keepers changing from using conventional bottom boards in the absence of varroamite.

Keywords: Apis mellifera, honey production, mesh bottom boards, tube bottom boards.

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1573 Fatigue Life of an Anti-Roll Bar of a Passenger Vehicle

Authors: J. Marzbanrad, A. Yadollahi

Abstract:

In the present paper, Fatigue life assessment of an anti-roll bar component of a passenger vehicle, is investigated by ANSYS 11 software. A stress analysis is also carried out by the finite element technique for the determination of highly stressed regions on the bar. Anti-roll bar is a suspension element used at the front, rear, or at both ends of a car that reduces body roll by resisting any unequal vertical motion between the pair of wheels to which it is connected. As a first stage, fatigue damage models proposed by some well-known references and the corresponding assumptions are discussed and some enhancements are proposed. Then, fracture analysis of an anti-roll bar of an automobile is carried out. The analysed type of the anti-roll bar is especially important as many cases are reported about the fracture after a 100,000 km of travel fatigue and fracture conditions. This paper demonstrates fatigue life of an anti-roll bar and then evaluated by experimental analytically results from other researcher.

Keywords: Anti-roll bar, Fracture, Fatigue life, Random loading

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1572 Technical Support of Intracranial Single Unit Activity Measurement

Authors: Richard Grünes, Karel Roubik

Abstract:

The article deals with technical support of intracranial single unit activity measurement. The parameters of the whole measuring set were tested in order to assure the optimal conditions of extracellular single-unit recording. Metal microelectrodes for measuring the single-unit were tested during animal experiments. From signals recorded during these experiments, requirements for the measuring set parameters were defined. The impedance parameters of the metal microelectrodes were measured. The frequency-gain and autonomous noise properties of preamplifier and amplifier were verified. The measurement and the description of the extracellular single unit activity could help in prognoses of brain tissue damage recovery.

Keywords: Measuring set, metal microelectrodes, single-unit, noise, impedance parameters, gain characteristics.

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1571 Design and Fabrication of a Scaffold with Appropriate Features for Cartilage Tissue Engineering

Authors: S. S. Salehi, A. Shamloo

Abstract:

Poor ability of cartilage tissue when experiencing a damage leads scientists to use tissue engineering as a reliable and effective method for regenerating or replacing damaged tissues. An artificial tissue should have some features such as biocompatibility, biodegradation and, enough mechanical properties like the original tissue. In this work, a composite hydrogel is prepared by using natural and synthetic materials that has high porosity. Mechanical properties of different combinations of polymers such as modulus of elasticity were tested, and a hydrogel with good mechanical properties was selected. Bone marrow derived mesenchymal stem cells were also seeded into the pores of the sponge, and the results showed the adhesion and proliferation of cells within the hydrogel after one month. In comparison with previous works, this study offers a new and efficient procedure for the fabrication of cartilage like tissue and further cartilage repair.

Keywords: Cartilage tissue engineering, hydrogel, mechanical strength, mesenchymal stem cell.

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1570 Exponentially Weighted Simultaneous Estimation of Several Quantiles

Authors: Valeriy Naumov, Olli Martikainen

Abstract:

In this paper we propose new method for simultaneous generating multiple quantiles corresponding to given probability levels from data streams and massive data sets. This method provides a basis for development of single-pass low-storage quantile estimation algorithms, which differ in complexity, storage requirement and accuracy. We demonstrate that such algorithms may perform well even for heavy-tailed data.

Keywords: Quantile estimation, data stream, heavy-taileddistribution, tail index.

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1569 Multi-criteria Optimization of Square Beam using Linear Weighted Average Model

Authors: Ali Farhaninejad, Rizal Zahari, Ehsan Rasooliyazdi

Abstract:

Increasing energy absorption is a significant parameter in vehicle design. Absorbing more energy results in decreasing occupant damage. Limitation of the deflection in a side impact results in decreased energy absorption (SEA) and increased peak load (PL). Hence a high crash force jeopardizes passenger safety and vehicle integrity. The aims of this paper are to determine suitable dimensions and material of a square beam subjected to side impact, in order to maximize SEA and minimize PL. To achieve this novel goal, the geometric parameters of a square beam are optimized using the response surface method (RSM).multi-objective optimization is performed, and the optimum design for different response features is obtained.

Keywords: Crashworthiness, side impact, energy absorption, multi-objective optimization, Square beam, SEA

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1568 The Collapse of a Crane on Site: A Case Study

Authors: T. Teruzzi, S. Antonietti, C. Mosca, C. Paglia

Abstract:

This paper discusses the causes of the structural failure in a tower crane. The structural collapse occurred at the upper joints of the extension element used to increase the height of the crane. The extension element consists of a steel lattice structure made with angular profiles and plates joined to the tower element by arc welding. Macroscopic inspection of the sections showed that the break was always observed on the angular profiles at the weld bead edge. The case study shows how, using mechanical characterization, chemical analysis of the steel and macroscopic and microscopic metallographic examinations, it was possible to obtain significant evidence that identified the mechanism causing the breakage. The analyses identified the causes of the structural failure as the use of materials that were not suitable for welding and poor performance in the welding joints.

Keywords: Failure, weld, microstructure, microcracks.

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1567 Design Exploration on Mixed-Use Development of Island House: Take the Southeast Coastal Area of Chinese as an Example

Authors: Fu Jiayan, Wang Zhu, Sun Jiaojiao

Abstract:

Mixed-use development is one of the most important trends in new island house transformation along southeast coastal area in China. Unique island geographical environment and profound fishing village culture coexist for a long time in this. With artistic creation for the purpose of the "live-work" houses are in a large number of emergence, however, still lack of systematic strategy. Based on space effect from marine resources to regional human settlements, this article teases out the evolution regularity of island settlement context and architectural form, then, puts forward the formation mechanism and construction model of art island houses. Thereby, to further explore space design method and site creation strategy of mixed-use development.

Keywords: Mixed-use, Island House, Art Creation, Southeast Coastal Area of Chinese.

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1566 Design and Analysis of an Electro Thermally Symmetrical Actuated Microgripper

Authors: Sh. Foroughi, V. Karamzadeh, M. Packirisamy

Abstract:

This paper presents design and analysis of an electrothermally symmetrical actuated microgripper applicable for performing micro assembly or biological cell manipulation. Integration of micro-optics with microdevice leads to achieve extremely precise control over the operation of the device. Geometry, material, actuation, control, accuracy in measurement and temperature distribution are important factors which have to be taken into account for designing the efficient microgripper device. In this work, analyses of four different geometries are performed by means of COMSOL Multiphysics 5.2 with implementing Finite Element Methods. Then, temperature distribution along the fingertip, displacement of gripper site as well as optical efficiency vs. displacement and electrical potential are illustrated. Results show in addition to the industrial application of this device, the usage of that as a cell manipulator is possible.

Keywords: Electro thermal actuator, MEMS, Microgripper, MOEMS.

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1565 Indonesian News Classification using Support Vector Machine

Authors: Dewi Y. Liliana, Agung Hardianto, M. Ridok

Abstract:

Digital news with a variety topics is abundant on the internet. The problem is to classify news based on its appropriate category to facilitate user to find relevant news rapidly. Classifier engine is used to split any news automatically into the respective category. This research employs Support Vector Machine (SVM) to classify Indonesian news. SVM is a robust method to classify binary classes. The core processing of SVM is in the formation of an optimum separating plane to separate the different classes. For multiclass problem, a mechanism called one against one is used to combine the binary classification result. Documents were taken from the Indonesian digital news site, www.kompas.com. The experiment showed a promising result with the accuracy rate of 85%. This system is feasible to be implemented on Indonesian news classification.

Keywords: classification, Indonesian news, text processing, support vector machine

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1564 Particle Image Velocimetry for Measuring Water Flow Velocity

Authors: King Kuok Kuok, Po Chan Chiu

Abstract:

Floods are natural phenomena, which may turn into disasters causing widespread damage, health problems and even deaths. Nowadays, floods had become more serious and more frequent due to climatic changes. During flooding, discharge measurement still can be taken by standing on the bridge across the river using portable measurement instrument. However, it is too dangerous to get near to the river especially during high flood. Therefore, this study employs Particle Image Velocimetry (PIV) as a tool to measure the surface flow velocity. PIV is a image processing technique to track the movement of water from one point to another. The PIV codes are developed using Matlab. In this study, 18 ping pong balls were scattered over the surface of the drain and images were taken with a digital SLR camera. The images obtained were analyzed using the PIV code. Results show that PIV is able to produce the flow velocity through analyzing the series of images captured.

Keywords: Particle Image Velocimetry, flow velocity, surface flow.

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1563 Variability of Soil Strength Parameters and its Effect on the Slope Stability of the Želazny Most Tailing Dam

Authors: Stella A. Arnaouti, Demos C. Angelides, Theodoros N. Chatzigogos, Witold M. Pytel

Abstract:

The Želazny Most tailing pond is one of the largest facilities worldwide for waste disposal from the copper mines located in South-West Poland. A potential failure of the dam would allow more than 10 million cubic meters of contaminated slurry to flow to the valley, causing immense environmental problems to the surrounding area. Thus, the determination of the strength properties of the dam's soils and their variability is of utmost importance. An extensive site investigation consisting of more than 480 cone penetration tests (CPTs) with or without pore water pressure measurements were conducted within a period of 13 years to study the mechanical properties of the tailings body. The present work investigates the point variability of the soil strength parameters (effective friction angle

Keywords: Soil strength variability, friction angle spatial variability, Želazny Most tailing dam.

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1562 Numerical Simulation of Restenosis in a Stented Coronary Artery

Authors: Weronika Kurowska-Nouyrigat, Jacek Szumbarski

Abstract:

Nowadays, cardiac disease is one of the most common cause of death. Each year almost one million of angioplasty interventions and stents implantations are made all over the world. Unfortunately, in 20-30% of cases neointimal proliferations leads to restenosis occurring within the following period of 3-6 months. Three major factors are believed to contribute mostly to the edge restenosis: (a) mechanical damage of the artery-s wall caused by the stent implantation, (b) interaction between the stent and the blood constituents and (c) endothelial growth stimulation by small (lower that 1.5 Pa) and oscillating wall shear stress. Assuming that this last actor is particularly important, a numerical model of restenosis basing on wall shear stress distribution in the stented artery was elaborated. A numerical simulations of the development of in-stent restenosis have been performed and realistic geometric patterns of a progressing lumen reduction have been obtained

Keywords: Coronary artery disease, coronary blood flow, instent restenosis.

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1561 Apoptotic Induction Ability of Harmalol and Its Binding: Biochemical and Biophysical Perspectives

Authors: Kakali Bhadra

Abstract:

Harmalol administration caused remarkable reduction in proliferation of HepG2 cells with GI50 of 14.2 mM, without showing much cytotoxicity in embryonic liver cell line, WRL-68. Data from circular dichroism and differential scanning calorimetric analysis of harmalol-CT DNA complex shows conformational changes with prominent CD perturbation and stabilization of CT DNA by 8 oC. Binding constant and stoichiometry was also calculated using the above biophysical techniques. Further, dose dependent apoptotic induction ability of harmalol was studied in HepG2 cells using different biochemical assays. Generation of ROS, DNA damage, changes in cellular external and ultramorphology, alteration of membrane, formation of comet tail, decreased mitochondrial membrane potential and a significant increase in Sub Go/G1 population made the cancer cell, HepG2, prone to apoptosis. Up regulation of p53 and caspase 3 further indicated the apoptotic role of harmalol.

Keywords: Apoptosis, beta carboline alkaloid, comet assay, cytotoxicity, ROS.

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