Technical Support of Intracranial Single Unit Activity Measurement
Authors: Richard Grünes, Karel Roubik
Abstract:
The article deals with technical support of intracranial single unit activity measurement. The parameters of the whole measuring set were tested in order to assure the optimal conditions of extracellular single-unit recording. Metal microelectrodes for measuring the single-unit were tested during animal experiments. From signals recorded during these experiments, requirements for the measuring set parameters were defined. The impedance parameters of the metal microelectrodes were measured. The frequency-gain and autonomous noise properties of preamplifier and amplifier were verified. The measurement and the description of the extracellular single unit activity could help in prognoses of brain tissue damage recovery.
Keywords: Measuring set, metal microelectrodes, single-unit, noise, impedance parameters, gain characteristics.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1327947
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[1] Dale, N., Pearson, T., Frenguelli, B. C.: Direct measurement of adenosine release during hypoxia in the CA1 region of the rat hippocampal slice. J Physiol, London, vol. 526, 2000, pp. 143-155.
[2] Pedata, F., Latini, S., Pugliesi, A. M., Pepeu, P.: Investigations into the adenosine outflow from hippocampal slices evoked by ischemia-like conditions. J Neurochem, vol. 61, 1993, pp. 284-289.
[3] Sattler, R., Xiong, Z., Lu, W-Y, MacDonald, J. F., Tymianski, M.: Distinct roles of synaptic and extrasynaptic NMDA receptors in excitotoxicity. J Neurosci, 2000, vol. 20, pp. 22-33.
[4] Nieber, K., Sevsic, J. a Illes, P.: Hypoxic changes in rat locus coeruleus neurons in vitro. J. Physiol., vol. 486, 1995, pp. 33-46.
[5] Englund, M., Bjurling, M., Edin, F., Hyllienmark, L., Brismar, T.: Hypoxic Excitability Changes and Sodium Currents in Hippocampus CA1 Neurons. Cellular and Molecular Neurobiology, vol. 24, no. 5, 2004, pp. 685-694.
[6] Sebasti├úo, A. M., de Mendonc┬© A., Moreira, T., Ribeiro, J. A.: Activation of Synaptic NMDA Receptors by Action Potential-Dependent Release of Transmitter during Hypoxia Impairs Recovery of Synaptic Transmission on Reoxygenation. The Journal of Neuroscience, November 1, vol. 21, no. 21, 2001, pp. 8564-8571.
[7] Hansen, A. J., Hounsgaard, J., Jahnsen, H.: Anoxia increases potassium conductance in hippocampal nerve cells. Acta physiol. Scand., vol. 115, 1982, pp. 301-310.
[8] Nadasdy Z., Csicsvari J., Penttonen M., Hetke J., Wise K., Buzsaki G.: Extracellular recording and analysis of neuronal activity: from single cells to ensembles. In: Neuronal Ensembles: Strategies for Recording and Decoding, edited by Eichenbaum HB and Davis JL. New York: Wiley-Liss, 1998, pp. 17-55.
[9] Vacul├¡n, ┼á., Fran─øk, M. and Rokyta, R.: Dorsal Rhizotomy Changes the Spontaneous Neuronal Activity of Nuclei in the Medial Thalamus. Physiological research, 49, 2000, pp. 279-283.
[10] Štork, M.: Jednodimenzionální nelineární digitální filtry I. AUTOMATIZACE, vol. 48, no. 6, 2005, pp. 382-385.
[11] Štork, M.: Jednodimenzionální nelineární digitální filtry II. AUTOMATIZACE, vol. 48, no. 7-8, 2005, pp. 446-450.