Search results for: Sangho Kang
284 Structural Analysis and Detail Design of APV Module Structure Using Topology Optimization Design
Authors: Hyun Kyu Cho, Jun Soo Kim, Young Hoon Lee, Sang Hoon Kang, Young Chul Park
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In the study, structure for one of offshore drilling system APV(Air Pressure Vessle) modules was designed by using topology optimum design and performed structural safety evaluation according to DNV rules. 3D model created base on design area and non-design area separated by using topology optimization for the environmental loads. This model separated 17 types for wind loads and dynamic loads and performed structural analysis evaluation for each model. As a result, the maximum stress occurred 181.25MPa.Keywords: APV, topology optimum design, DNV, structural analysis, stress
Procedia PDF Downloads 423283 A Study on the Pulse Transformer Design Considering Inrush Current in the Welding Machine
Authors: In-Gun Kim, Hyun-Seok Hong, Dong-Woo Kang, Ju Lee
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An Inverter type arc-welding machine is inclined to be designed for higher frequency in order to reduce the size and cost. The need of the core material reconsideration for high frequency pulse transformer is more important since core loss grows as the frequency rises. An arc welding machine’s pulse transformer is designed using an Area Product (Ap) method and is considered margin air gap core design in order to prevent the burning of the IGBT by the inrush current. Finally, the reduction of the core weight and the core size are compared according to different materials for 30kW inverter type arc welding machine.Keywords: pulse transformers, welding, inrush current, air gaps
Procedia PDF Downloads 456282 Phase Transition of Aqueous Ternary (THF + Polyvinylpyrrolidone + H2O) System as Revealed by Terahertz Time-Domain Spectroscopy
Authors: Hyery Kang, Dong-Yeun Koh, Yun-Ho Ahn, Huen Lee
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Determination of the behavior of clathrate hydrate with inhibitor in the THz region will provide useful information about hydrate plug control in the upstream of the oil and gas industry. In this study, terahertz time-domain spectroscopy (THz-TDS) revealed the inhibition of the THF clathrate hydrate system with dosage of polyvinylpyrrolidone (PVP) with three different molecular weights. Distinct footprints of phase transition in the THz region (0.4–2.2 THz) were analyzed and absorption coefficients and real part of refractive indices are obtained in the temperature range of 253 K to 288 K. Along with the optical properties, ring breathing and stretching modes for different molecular weights of PVP in THF hydrate are analyzed by Raman spectroscopy.Keywords: clathrate hydrate, terahertz spectroscopy, tetrahydrofuran, inhibitor
Procedia PDF Downloads 338281 Improving Short-Term Forecast of Solar Irradiance
Authors: Kwa-Sur Tam, Byung O. Kang
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By using different ranges of daily sky clearness index defined in this paper, any day can be classified as a clear sky day, a partly cloudy day or a cloudy day. This paper demonstrates how short-term forecasting of solar irradiation can be improved by taking into consideration the type of day so defined. The source of day type dependency has been identified. Forecasting methods that take into consideration of day type have been developed and their efficacy have been established. While all methods that implement some form of adjustment to the cloud cover forecast provided by the U.S. National Weather Service provide accuracy improvement, methods that incorporate day type dependency provides even further improvement in forecast accuracy.Keywords: day types, forecast methods, National Weather Service, sky cover, solar energy
Procedia PDF Downloads 465280 Remanufacturing and Integrity Assessment of a 27-Year-Old 25kV Gas Insulated Switchgear: A Comprehensive Study on Dismantling, Inspection, and Testing
Authors: Yechan Kim, Bonhyuk Ku, Minkyung Jung, Hyoungku Kang
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This study presents the remanufacturing of a 25kV gas insulated switchgear (GIS) that operated indoors for 27 years before being decommissioned due to aging. The research involved a detailed process of dismantling, visual inspection, component-wise examination, and various testing methodologies to assess the equipment's condition. The focus was on evaluating the GIS's integrity and feasibility for remanufacturing. The results highlight the potential of remanufacturing in extending the life of electrical power equipment, offering insights into the best practices, challenges, and technical considerations of such an undertaking. This contributes to a sustainable approach in the power industry, emphasizing the reuse and restoration of aging equipment.Keywords: remanufacturing, dismantling, gas insulated switchgear, sustainability, life extension
Procedia PDF Downloads 75279 Effects of Macrophyte Vallisneria asiatica Biomasses on the Algae Community
Authors: Caixia Kang, Takahiro Kuba, Aimin Hao, Yasushi Iseri, Chunjie Li, Zhenjia Zhang
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To improve the water quality of lakes and control algae blooms, The effects of Vallisneria asiatica which is one of aquatic plants spread over Lake Taihu. With different biomasses on the water quality and algae communities were researched. The results indicated that V. asiatica could control an excess of Microcystis spp. When the V. asiatica biomass was larger than 50g in the tank with 30L solution in the laboratory, Planktonic and epiphytic algae responded differently to V. asiatica. The presence of macrophyte V. asiatica in eutrophic waters has a positive effect on algae compositions because of different sensitivities of algae species to allelopathic substances released by macrophyte V. asiatica. That is, V. asiatica could inhibit the growth of Microcystis spp. effectively and was benefited to the diatom on the condition in the laboratory.Keywords: algae bloom, algae community, Microcystis spp., Vallisneria asiatica
Procedia PDF Downloads 380278 Construction Information Visualization System Using nD CAD Model
Authors: Hyeon-seoung Kim, Sang-mi Park, Sun-ju Han, Leen-seok Kang
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The visualization technology of construction information using 3D and nD modeling can satisfy the visualization needs of each construction project participant. The nD CAD system is a tool that the construction information, such as construction schedule, cost and resource utilization, are simulated by 4D, 5D and 6D object formats based on 3D object. This study developed a methodology and simulation engine for nD CAD system for construction project management. It has improved functions such as built-in schedule generation, cost simulation of changed budget and built-in resource allocation comparing with the current systems. To develop an integrated nD CAD system, this study attempts an integrated method to link 5D and 6D objects based on 4D object.Keywords: building information modeling, visual simulation, 3D object, nD CAD augmented reality
Procedia PDF Downloads 311277 Time-Domain Simulations of the Coupled Dynamics of Surface Riding Wave Energy Converter
Authors: Chungkuk Jin, Moo-Hyun Kim, HeonYong Kang
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A surface riding (SR) wave energy converter (WEC) is designed and its feasibility and performance are numerically simulated by the author-developed floater-mooring-magnet-electromagnetics fully-coupled dynamic analysis computer program. The biggest advantage of the SR-WEC is that the performance is equally effective even in low sea states and its structural robustness is greatly improved by simply riding along the wave surface compared to other existing WECs. By the numerical simulations and actuator testing, it is clearly demonstrated that the concept works and through the optimization process, its efficiency can be improved.Keywords: computer simulation, electromagnetics fully-coupled dynamics, floater-mooring-magnet, optimization, performance evaluation, surface riding, WEC
Procedia PDF Downloads 144276 Bending and Shear Characteristics of Hollowcore Slab with Polystyrene Forms
Authors: Kang Kun Lee
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New I-slab system with polystyrene forms and precast concrete deck is proposed to reduce the construction period and the self-weight of the slab. This paper presents experimental works on the bending and shear of the I-slabs. Five specimens were tested. The main parameters of experiments are diameters of the holes made by polystyrene form and the thickness of slab. Structural performance of I-slab is evaluated on the basis of failure mode, load-displacement curve, and ultimate strengths. Based on the test results, it is found that the critical punching shear sections are changed as the test variables are different, hence resulting in the varied punching shear strengths. Test results indicate that the developed I-slab is very effective to increase the strength due to self-weight reduction.Keywords: hollowcore slab, section force-deformation response, precast concrete deck
Procedia PDF Downloads 388275 Production of Natural Gas Hydrate by Using Air and Carbon Dioxide
Authors: Yun-Ho Ahn, Hyery Kang, Dong-Yeun Koh, Huen Lee
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In this study, we demonstrate the production of natural gas hydrates from permeable marine sediments with simultaneous mechanisms for methane recovery and methane-air or methane-air/carbon dioxide replacement. The simultaneous melting happens until the chemical potentials become equal in both phases as natural gas hydrate depletion continues and self-regulated methane-air replacement occurs over an arbitrary point. We observed certain point between dissociation and replacement mechanisms in the natural gas hydrate reservoir, and we call this boundary as critical methane concentration. By the way, when carbon dioxide was added, the process of chemical exchange of methane by air/carbon dioxide was observed in the natural gas hydrate. The suggested process will operate well for most global natural gas hydrate reservoirs, regardless of the operating conditions or geometrical constraints.Keywords: air injection, carbon dioxide sequestration, hydrate production, natural gas hydrate
Procedia PDF Downloads 457274 Investigating the Demand of Short-Shelf Life Food Products for SME Wholesalers
Authors: Yamini Raju, Parminder S. Kang, Adam Moroz, Ross Clement, Alistair Duffy, Ashley Hopwell
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Accurate prediction of fresh produce demand is one the challenges faced by Small Medium Enterprise (SME) wholesalers. Current research in this area focused on limited number of factors specific to a single product or a business type. This paper gives an overview of the current literature on the variability factors used to predict demand and the existing forecasting techniques of short shelf life products. It then extends it by adding new factors and investigating if there is a time lag and possibility of noise in the orders. It also identifies the most important factors using correlation and Principal Component Analysis (PCA).Keywords: demand forecasting, deteriorating products, food wholesalers, principal component analysis, variability factors
Procedia PDF Downloads 519273 Iterative Panel RC Extraction for Capacitive Touchscreen
Authors: Chae Hoon Park, Jong Kang Park, Jong Tae Kim
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Electrical characteristics of capacitive touchscreen need to be accurately analyzed to result in better performance for multi-channel capacitance sensing. In this paper, we extracted the panel resistances and capacitances of the touchscreen by comparing measurement data and model data. By employing a lumped RC model for driver-to-receiver paths in touchscreen, we estimated resistance and capacitance values according to the physical lengths of channel paths which are proportional to the RC model. As a result, we obtained the model having 95.54% accuracy of the measurement data.Keywords: electrical characteristics of capacitive touchscreen, iterative extraction, lumped RC model, physical lengths of channel paths
Procedia PDF Downloads 333272 Automated Recognition of Still’s Murmur in Children
Authors: Sukryool Kang, James McConnaughey, Robin Doroshow, Raj Shekhar
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Still’s murmur, a vibratory heart murmur, is the most common normal innocent murmur of childhood. Many children with this murmur are unnecessarily referred for cardiology consultation and testing, which exacts a high cost financially and emotionally on the patients and their parents. Pediatricians to date are not successful at distinguishing Still’s murmur from murmurs of true heart disease. In this paper, we present a new algorithmic approach to distinguish Still’s murmur from pathological murmurs in children. We propose two distinct features, spectral width and signal power, which describe the sharpness of the spectrum and the signal intensity of the murmur, respectively. Seventy pediatric heart sound recordings of 41 Still’s and 29 pathological murmurs were used to develop and evaluate our algorithm that achieved a true positive rate of 97% and false positive rate of 0%. This approach would meet clinical standards in recognizing Still’s murmur.Keywords: AR modeling, auscultation, heart murmurs, Still's murmur
Procedia PDF Downloads 366271 Nondestructive Natural Gas Hydrate Production by Using Air and Carbon Dioxide
Authors: Ahn Yun-Ho, Hyery Kang, Koh Dong-Yeun, Huen Lee
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In this study, we demonstrate the production of natural gas hydrates from permeable marine sediments with simultaneous mechanisms for methane recovery and methane-air or methane-air/carbon dioxide replacement. The simultaneous melting happens until the chemical potentials become equal in both phases as natural gas hydrate depletion continues and self-regulated methane-air replacement occurs over an arbitrary point. We observed certain point between dissociation and replacement mechanisms in the natural gas hydrate reservoir, and we call this boundary as critical methane concentration. By the way, when carbon dioxide was added, the process of chemical exchange of methane by air/carbon dioxide was observed in the natural gas hydrate. The suggested process will operate well for most global natural gas hydrate reservoirs, regardless of the operating conditions or geometrical constraints.Keywords: air injection, carbon dioxide sequestration, hydrate production, natural gas hydrate
Procedia PDF Downloads 572270 Robust Speed Sensorless Control to Estimated Error for PMa-SynRM
Authors: Kyoung-Jin Joo, In-Gun Kim, Hyun-Seok Hong, Dong-Woo Kang, Ju Lee
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Recently, the permanent magnet-assisted synchronous reluctance motor (PMa-SynRM) that can be substituted for the induction motor has been studying because of the needs of the development of the premium high efficiency motor for the minimum energy performance standard (MEPS). PMa-SynRM is required to the speed and position information for motor speed and torque controls. However, to apply the sensors has many problems that are sensor mounting space shortage and additional cost, etc. Therefore, in this paper, speed-sensorless control based on model reference adaptive system (MRAS) is introduced to eliminate the sensor. The sensorless method is constructed in a reference model as standard and an adaptive model as the state observer. The proposed algorithm is verified by the simulation.Keywords: PMa-SynRM, sensorless control, robust estimation, MRAS method
Procedia PDF Downloads 403269 Capacitive Coupling Wireless Power Transfer System with 6.78 MHz Class D Inverter
Authors: Kang Hyun Yi
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Wireless power transfer technologies are inductive coupling, magnetic resonance, and capacitive coupling methods, typically. Among them, the capacitive coupling wireless power transfer, also named Capacitive Coupling Wireless Power Transfer (CCWPT), has been researched to overcome the drawbacks of other approaches. The CCWPT has many advantages such as a simple structure, low standing power loss, reduced Electromagnetic Interference (EMI) and the ability to transfer power through metal barriers. In this paper, the CCWPT system with 6.78MHz class D inverter is proposed and analyzed. The proposed system is consisted of the 6.78MHz class D inverter with the LC low pass filter, the capacitor between a transmitter and a receiver and impedance transformers. The system is verified with a prototype for charging mobile devices.Keywords: wireless power transfer, capacitive coupling power transfer, class D inverter, 6.78MHz
Procedia PDF Downloads 647268 Dynamic Modeling of Wind Farms in the Jeju Power System
Authors: Dae-Hee Son, Sang-Hee Kang, Soon-Ryul Nam
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In this paper, we develop a dynamic modeling of wind farms in the Jeju power system. The dynamic model of wind farms is developed to study their dynamic effects on the Jeju power system. PSS/E is used to develop the dynamic model of a wind farm composed of 1.5-MW doubly fed induction generators. The output of a wind farm is regulated based on pitch angle control, in which the two controllable parameters are speed and power references. The simulation results confirm that the pitch angle is successfully controlled, regardless of the variation in wind speed and output regulation.Keywords: dynamic model, Jeju power system, online limitation, pitch angle control, wind farm
Procedia PDF Downloads 325267 BIM Application and Construction Schedule Simulation for the Horizontal Work Area
Authors: Hyeon-Seong Kim, Sang-Mi Park, Seul-Gi Kim, Seon-Ju Han, Leen-Seok Kang
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The use of BIM, including 4D CAD system, in a construction project is gradually increasing. Since the building construction works repeatedly in the vertical space, it is relatively easy to confirm the interference effect when applying the BIM, but the interference effect for the civil engineering project is relatively small because the civil works perform non-repetitive processes in the horizontal space. For this reason, it is desirable to apply BIM to the construction phase when applying BIM to the civil engineering project, and the most active BIM tool applied to the construction phase is the 4D CAD function for the schedule management. This paper proposes the application procedure of BIM by the construction phase of civil engineering project and a linear 4D CAD construction methodology suitable for the civil engineering project in which linear work is performed.Keywords: BIM, 4D CAD, linear 4D simulation, VR
Procedia PDF Downloads 398266 Off-Line Parameter Estimation for the Induction Motor Drive System
Authors: Han-Woong Ahn, In-Gun Kim, Hyun-Seok Hong, Dong-Woo Kang, Ju Lee
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It is important to accurately identify machine parameters for direct vector control. To obtain the parameter values, traditional methods can be used such as no-load and rotor locked tests. However, there are many differences between values obtained from the traditional tests and actual values. In addition, there are drawbacks that additional equipment and cost are required for the experiment. Therefore, it is hard to temporary operation to estimate induction motor parameters. Therefore, this paper deals with the estimation algorithm of induction motor parameters without a motor operation and the measurement from additional equipment such as sensors and dynamometer. The validity and usefulness of the estimation algorithm considering inverter nonlinearity is verified by comparing the conventional method with the proposed method.Keywords: induction motor, parameter, off-line estimation, inverter nonlinearity
Procedia PDF Downloads 527265 Optimal Rotor Design of an 150kW-Class IPMSM through the 3D Voltage-Inductance Map Analysis Method
Authors: Eung-Seok Park, Tae-Chul Jeong, Hyun-Jong Park, Hyun-Woo Jun, Dong-Woo Kang, Ju Lee
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This presents a methodology to determine detail design directions of an 150kW-class IPMSM (interior permanent magnet synchronous motor) and its detail design. The basic design of the stator and rotor was conducted. After dividing the designed models into the best cases and the worst cases based on rotor shape parameters, Sensitivity analysis and 3D Voltage-Inductance Map (3D EL-Map) parameters were analyzed. Then, the design direction for the final model was predicted. Based on the prediction, the final model was extracted with Trend analysis. Lastly, the final model was validated with experiments.Keywords: PMSM, optimal design, rotor design, voltage-inductance map
Procedia PDF Downloads 673264 An Online Mastery Learning Method Based on a Dynamic Formative Evaluation
Authors: Jeongim Kang, Moon Hee Kim, Seong Baeg Kim
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This paper proposes a novel e-learning model that is based on a dynamic formative evaluation. On evaluating the existing format of e-learning, conditions regarding repetitive learning to achieve mastery, causes issues for learners to lose tension and become neglectful of learning. The dynamic formative evaluation proposed is able to supplement limitation of the existing approaches. Since a repetitive learning method does not provide a perfect feedback, this paper puts an emphasis on the dynamic formative evaluation that is able to maximize learning achievement. Through the dynamic formative evaluation, the instructor is able to refer to the evaluation result when making estimation about the learner. To show the flow chart of learning, based on the dynamic formative evaluation, the model proves its effectiveness and validity.Keywords: online learning, dynamic formative evaluation, mastery learning, repetitive learning method, learning achievement
Procedia PDF Downloads 508263 Study of Biofouling Wastewater Treatment Technology
Authors: Sangho Park, Mansoo Kim, Kyujung Chae, Junhyuk Yang
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The International Maritime Organization (IMO) recognized the problem of invasive species invasion and adopted the "International Convention for the Control and Management of Ships' Ballast Water and Sediments" in 2004, which came into force on September 8, 2017. In 2011, the IMO approved the "Guidelines for the Control and Management of Ships' Biofouling to Minimize the Transfer of Invasive Aquatic Species" to minimize the movement of invasive species by hull-attached organisms and required ships to manage the organisms attached to their hulls. Invasive species enter new environments through ships' ballast water and hull attachment. However, several obstacles to implementing these guidelines have been identified, including a lack of underwater cleaning equipment, regulations on underwater cleaning activities in ports, and difficulty accessing crevices in underwater areas. The shipping industry, which is the party responsible for understanding these guidelines, wants to implement them for fuel cost savings resulting from the removal of organisms attached to the hull, but they anticipate significant difficulties in implementing the guidelines due to the obstacles mentioned above. Robots or people remove the organisms attached to the hull underwater, and the resulting wastewater includes various species of organisms and particles of paint and other pollutants. Currently, there is no technology available to sterilize the organisms in the wastewater or stabilize the heavy metals in the paint particles. In this study, we aim to analyze the characteristics of the wastewater generated from the removal of hull-attached organisms and select the optimal treatment technology. The organisms in the wastewater generated from the removal of the attached organisms meet the biological treatment standard (D-2) using the sterilization technology applied in the ships' ballast water treatment system. The heavy metals and other pollutants in the paint particles generated during removal are treated using stabilization technologies such as thermal decomposition. The wastewater generated is treated using a two-step process: 1) development of sterilization technology through pretreatment filtration equipment and electrolytic sterilization treatment and 2) development of technology for removing particle pollutants such as heavy metals and dissolved inorganic substances. Through this study, we will develop a biological removal technology and an environmentally friendly processing system for the waste generated after removal that meets the requirements of the government and the shipping industry and lays the groundwork for future treatment standards.Keywords: biofouling, ballast water treatment system, filtration, sterilization, wastewater
Procedia PDF Downloads 109262 Study on Network-Based Technology for Detecting Potentially Malicious Websites
Authors: Byung-Ik Kim, Hong-Koo Kang, Tae-Jin Lee, Hae-Ryong Park
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Cyber terrors against specific enterprises or countries have been increasing recently. Such attacks against specific targets are called advanced persistent threat (APT), and they are giving rise to serious social problems. The malicious behaviors of APT attacks mostly affect websites and penetrate enterprise networks to perform malevolent acts. Although many enterprises invest heavily in security to defend against such APT threats, they recognize the APT attacks only after the latter are already in action. This paper discusses the characteristics of APT attacks at each step as well as the strengths and weaknesses of existing malicious code detection technologies to check their suitability for detecting APT attacks. It then proposes a network-based malicious behavior detection algorithm to protect the enterprise or national networks.Keywords: Advanced Persistent Threat (APT), malware, network security, network packet, exploit kits
Procedia PDF Downloads 365261 Development of a Human Vibration Model Considering Muscles and Stiffness of Intervertebral Discs
Authors: Young Nam Jo, Moon Jeong Kang, Hong Hee Yoo
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Most human vibration models have been modeled as a multibody system consisting of some rigid bodies and spring-dampers. These models are developed for certain posture and conditions. So, the models cannot be used in vibration analysis in various posture and conditions. The purpose of this study is to develop a human vibration model that represent human vibration characteristics under various conditions by employing a musculoskeletal model. To do this, the human vibration model is developed based on biomechanical models. In addition, muscle models are employed instead of spring-dampers. Activations of muscles are controlled by PD controller to maintain body posture under vertical vibration is applied. Each gain value of the controller is obtained to minimize the difference of apparent mass and acceleration transmissibility between experim ent and analysis by using an optimization method.Keywords: human vibration analysis, hill type muscle model, PD control, whole-body vibration
Procedia PDF Downloads 448260 Application of Blockchain Technology in Geological Field
Authors: Mengdi Zhang, Zhenji Gao, Ning Kang, Rongmei Liu
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Management and application of geological big data is an important part of China's national big data strategy. With the implementation of a national big data strategy, geological big data management becomes more and more critical. At present, there are still a lot of technology barriers as well as cognition chaos in many aspects of geological big data management and application, such as data sharing, intellectual property protection, and application technology. Therefore, it’s a key task to make better use of new technologies for deeper delving and wider application of geological big data. In this paper, we briefly introduce the basic principle of blockchain technology at the beginning and then make an analysis of the application dilemma of geological data. Based on the current analysis, we bring forward some feasible patterns and scenarios for the blockchain application in geological big data and put forward serval suggestions for future work in geological big data management.Keywords: blockchain, intellectual property protection, geological data, big data management
Procedia PDF Downloads 87259 Effect of Process Parameters on Mechanical Properties of Friction Stir Welded Aluminium Alloy Joints Using Factorial Design
Authors: Gurjinder Singh, Ankur Gill, Amardeep Singh Kang
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In the present work an effort has been made to study the influence of the welding parameters on tensile strength of friction stir welding of aluminum. Three process parameters tool rotation speed, welding speed, and shoulder diameter were selected for the study. Two level factorial design of eight runs was selected for conducting the experiments. The mathematical model was developed from the data obtained. The significance of coefficients and adequacy of developed models were tested by ‘t’ test and ‘F’ test respectively. The effects of process parameters on mechanical properties have been represented in the form of graphs for better understanding.Keywords: friction stir welding, aluminium alloy, mathematical model, welding speed
Procedia PDF Downloads 439258 Natural Frequency Analysis of Small-Scale Arch Structure by Shaking Table Test
Authors: Gee-Cheol Kim, Joo-Won Kang
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Structural characteristics of spatial structure are different from that of rahmen structures and it has many factors that are unpredictable experientially. Both horizontal and vertical earthquake should be considered because of seismic behaviour characteristics of spatial structures. This experimental study is conducted about seismic response characteristics of roof structure according to the effect of columns or walls, through scale model of arch structure that has the basic dynamic characteristics of spatial structure. Though remarkable response is not occurred for horizontal direction in the region of higher frequency than the region of frequency that seismic energy is concentrated, relatively large response is occurred in vertical direction. It is proved that seismic response of arch structure with column is varied according to property of column.Keywords: arch structure, seismic response, shaking table, spatial structure
Procedia PDF Downloads 365257 Introduction of PMMA-Tag to VHH for Improving Recovery and Immobilization Rate of VHHS
Authors: Bongmun Kang, Kagnari Yamakawa, Yoshihisa Hagihara, Yuji Ito, Michimasa Kishimoto, Yoichi Kumada
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The PMMA-tag was genetically fused with the C-terminal region of VHH molecules. This antibody, VHH, is known as a single-chain domain, which is devoid of light chains. The PMMA-tag, which could affect the isoelectric point (pI) changeable with a charge of amino acid in VHHs were closely related to the solubility of VHH molecules during refolding. The genetic fusion of PMMA-tag to C-terminal region of VHHs significantly affects the recovery of their soluble protein during refolding by 50 mM TAPS at pH 8.5. It could be refolded with a recovery of more than 95% by dialysis at pH 8.5. A marked difference in the antigen-binding activities in the adsorption state was significantly high in VHH-PM compared to the wild type of VHH. There are approximately 8-fold differences in the antigen-binding activities in the adsorption state between VHH-PM and VHH.Keywords: VHH, PMMA-tag, isoelectric point, pH, Solubility, refolding, immobilization, ELISA
Procedia PDF Downloads 418256 Production of Recombinant VP2 Protein of Canine Parvovirus 2a Using Baculovirus Expression System
Authors: Soo Dong Cho, In-Ohk Ouh, Byeong Sul Kang, Seyeon Park, In-Soo Cho, Jae Young Song
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An VP2 gene from the current prevalent CPV (Canine Parvovirus) strain (new CPV-2a) in the Republic of Korea was expressed in a baculovirus expression system. Genomic DNA was extracted from the isolate strain CPV-2a. The recombinant baculovirus, containing the coding sequences of VP2 with the histidine tag at the N-terminus, were generated by using the Bac-to-Bac system. For production of the recombinant VP2 proteins, SF9 cells were transfection into 6 wells. Propagation of recombinant baculoviruses and expression of the VP2 protein were performed in the Sf9 cell line maintained. The proteins were detected to Western blot anlaysis. CPV-2a VP2 was detected by Western blotting the monoclonal antibodies recognized 6x His and the band had a molecular weight of 65 KDa. We demonstrated that recombinant CPV-2a VP2 expression in baculovirus. The recombinant CPV-2a VP2 may able to development of specific diagnostic test and vaccination of against CPV2. This study provides a foundation for application of CPV2 on the development of new CPV2 subunit vaccine.Keywords: baculovirus, canine parvovirus 2a, Dog, Korea
Procedia PDF Downloads 242255 Development of Construction Cost Optimization System Using Genetic Algorithm Method
Authors: Hyeon-Seung Kim, Young-Hwan Kim, Sang-Mi Park, Min-Seo Kim, Jong-Myeung Shin, Leen-Seok Kang
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The project budget at the planned stage might be changed by the insufficient government budget or the design change. There are many cases more especially in the case of a project performed for a long period of time. If the actual construction budget is insufficient comparing with the planned budget, the construction schedule should also be changed to match the changed budget. In that case, most project managers change the planned construction schedule by a heuristic approach without a reasonable consideration on the work priority. This study suggests an optimized methodology to modify the construction schedule according to the changed budget. The genetic algorithm was used to optimize the modified construction schedule within the changed budget. And a simulation system of construction cost histogram in accordance with the construction schedule was developed in the BIM (Building Information Modeling) environment.Keywords: 5D, BIM, GA, cost optimization
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