Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 653

Search results for: Mohammad Amin

653 Augmenting Use Case View for Modeling

Authors: Pradip Peter Dey, Bhaskar Raj Sinha, Mohammad Amin, Hassan Badkoobehi

Abstract:

Mathematical, graphical and intuitive models are often constructed in the development process of computational systems. The Unified Modeling Language (UML) is one of the most popular modeling languages used by practicing software engineers. This paper critically examines UML models and suggests an augmented use case view with the addition of new constructs for modeling software. It also shows how a use case diagram can be enhanced. The improved modeling constructs are presented with examples for clarifying important design and implementation issues.

Keywords: Software architecture, software design, Unified Modeling Language (UML), user interface.

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652 Thermodynamic Analysis of an Ejector-Absorption Refrigeration Cycle with Using NH3-H2O

Authors: Samad Jafarmadar, Amin Habibzadeh, Mohammad Mehdi Rashidi, Sayed Sina Rezaei, Abbas Aghagoli

Abstract:

In this paper, the ejector-absorption refrigeration cycle is presented. This article deals with the thermodynamic simulation and the first and second law analysis of an ammonia-water. The effects of parameters such as condenser, absorber, generator, and evaporator temperatures have been investigated. The influence of the various operating parameters on the performance coefficient and exergy efficiency of this cycle has been studied. The results show that when the temperature of different parts increases, the performance coefficient and the exergy efficiency of the cycle decrease, except for evaporator and generator, that causes an increase in coefficient of performance (COP). According to the results, absorber and ejector have the highest exergy losses in the studied conditions.

Keywords: Absorption refrigeration, COP, ejector, exergy efficiency.

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651 A CDA-Driven Study of World English Series Published by Cengage Heinle

Authors: Mohammad Amin Mozaheb, Jalal Farzaneh Dehkordi, Khojasteh Hosseinzadehpilehvar

Abstract:

English Language Teaching (ELT) is widely promoted across the world. ELT textbooks play pivotal roles in the mentioned process. Since biases of authors have been an issue of continuing interest to analysts over the past few years, the present study seeks to analyze an ELT textbook using Critical Discourse Analysis (CDA). To obtain the goal of the study, the listening section of a book called World English 3 (new edition) has been analyzed in terms of the cultures and countries mentioned in the listening section of the book using content-based analysis. The analysis indicates biases towards certain cultures. Moreover, some countries are shown as rich and powerful countries, while some others have been shown as poor ones without considering the history behind them.

Keywords: ELT, textbooks, critical discourse analysis, World English.

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650 Traffic Noise under Stop and Go Conditions in Intersections – A Case Study

Authors: Nima Jahandar, Amin Hosseinpour, Mohammad Ali Sahraei

Abstract:

Whit the increasing of traffic, noise emanated from motor vehicles increases as well, which subsequently causes adding to the stress of modern city. Thus, it is needed to look for most critical areas in terms of environmental and social impact of noise. There are several critical situations for noise emanated from motor vehicles such as stop and go situation which usually occurs near junctions or at-grade intersections. This study was conducted in two locations, most common types of intersections, crossroads and Tjunctions. The highest average noise levels are recorded during Go phase for T-junction, 64.4 dB, and Drive phase for crossroad, 64 dB. It implies that the existence of intersection caused the noise level to increase. The vehicles starting to move produce more sound than when they travel at a constant speed along the intersection. It is suggested that special considerations and priority of allocating funds should be given to these critical spots.

Keywords: Crossroad, T-junction, Traffic Noise, Stop and Go, Urban noise

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649 An Exact MCNP Modeling of Pebble Bed Reactors

Authors: Amin Abedi, Naser Vosoughi, Mohammad Bagher Ghofrani

Abstract:

Double heterogeneity of randomly located pebbles in the core and Coated Fuel Particles (CFPs) in the pebbles are specific features in pebble bed reactors and usually, because of difficulty to model with MCNP code capabilities, are neglected. In this study, characteristics of HTR-10, Tsinghua University research reactor, are used and not only double heterogeneous but also truncated CFPs and Pebbles are considered.Firstly, 8335 CFPs are distributed randomly in a pebble and then the core of reactor is filled with those pebbles and graphite pebbles as moderator such that 57:43 ratio of fuel and moderator pebbles is established.Finally, four different core configurations are modeled. They are Simple Cubic (SC) structure with truncated pebbles,SC structure without truncated pebble, and Simple Hexagonal(SH) structure without truncated pebbles and SH structure with truncated pebbles. Results like effective multiplication factor (Keff), critical height,etc. are compared with available data.

Keywords: Double Heterogeneity, HTR-10, MCNP, Pebble Bed Reactor, Stochastic Geometry.

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648 Application of Wavelet Neural Networks in Optimization of Skeletal Buildings under Frequency Constraints

Authors: Mohammad Reza Ghasemi, Amin Ghorbani

Abstract:

The main goal of the present work is to decrease the computational burden for optimum design of steel frames with frequency constraints using a new type of neural networks called Wavelet Neural Network. It is contested to train a suitable neural network for frequency approximation work as the analysis program. The combination of wavelet theory and Neural Networks (NN) has lead to the development of wavelet neural networks. Wavelet neural networks are feed-forward networks using wavelet as activation function. Wavelets are mathematical functions within suitable inner parameters, which help them to approximate arbitrary functions. WNN was used to predict the frequency of the structures. In WNN a RAtional function with Second order Poles (RASP) wavelet was used as a transfer function. It is shown that the convergence speed was faster than other neural networks. Also comparisons of WNN with the embedded Artificial Neural Network (ANN) and with approximate techniques and also with analytical solutions are available in the literature.

Keywords: Weight Minimization, Frequency Constraints, Steel Frames, ANN, WNN, RASP Function.

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647 Advances on LuGre Friction Model

Authors: Mohammad Fuad Mohammad Naser, Faycal Ikhouane

Abstract:

LuGre friction model is an ordinary differential equation that is widely used in describing the friction phenomenon for mechanical systems. The importance of this model comes from the fact that it captures most of the friction behavior that has been observed including hysteresis. In this paper, we study some aspects related to the hysteresis behavior induced by the LuGre friction model.

Keywords: Hysteresis, LuGre model, operator, (strong) consistency.

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646 Adaptive Car Safety System

Authors: Shahram Jafari, Mohammad-Ali Nikouei Mahani, Mohammad Arabnezhad, Mahdi Sharifi

Abstract:

Car accident is one of the major causes of death in many countries. Many researchers have attempted to design and develop techniques to increase car safety in the past recent years. In spite of all the efforts, it is still challenging to design a system adaptive to the driver rather than the automotive characteristics. In this paper, the adaptive car safety system is explained which attempts to find a balance.

Keywords: Analog to Digital Converter (ADC), AdaptiveCar Safety System, Multi-Media Card (MMC).

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645 New Exact Solutions for the (3+1)-Dimensional Breaking Soliton Equation

Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi

Abstract:

In this work, we obtain some analytic solutions for the (3+1)-dimensional breaking soliton after obtaining its Hirota-s bilinear form. Our calculations show that, three-wave method is very easy and straightforward to solve nonlinear partial differential equations.

Keywords: (3+1)-dimensional breaking soliton equation, Hirota'sbilinear form.

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644 Using FEM for Prediction of Thermal Post-Buckling Behavior of Thin Plates During Welding Process

Authors: Amin Esmaeilzadeh, Mohammad Sadeghi, Farhad Kolahan

Abstract:

Arc welding is an important joining process widely used in many industrial applications including production of automobile, ships structures and metal tanks. In welding process, the moving electrode causes highly non-uniform temperature distribution that leads to residual stresses and different deviations, especially buckling distortions in thin plates. In order to control the deviations and increase the quality of welded plates, a fixture can be used as a practical and low cost method with high efficiency. In this study, a coupled thermo-mechanical finite element model is coded in the software ANSYS to simulate the behavior of thin plates located by a 3-2-1 positioning system during the welding process. Computational results are compared with recent similar works to validate the finite element models. The agreement between the result of proposed model and other reported data proves that finite element modeling can accurately predict the behavior of welded thin plates.

Keywords: Welding, thin plate, buckling distortion, fixture locators, finite element modelling.

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643 Some Complexiton Type Solutions of the (3+1)-Dimensional Jimbo-Miwa Equation

Authors: Mohammad Taghi Darvishi, Mohammad Najafi

Abstract:

By means of the extended homoclinic test approach (shortly EHTA) with the aid of a symbolic computation system such as Maple, some complexiton type solutions for the (3+1)-dimensional Jimbo-Miwa equation are presented.

Keywords: Jimbo-Miwa equation, painleve analysis, Hirota's bilinear form, computerized symbolic computation.

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642 The Effect of Failure Rate on Repair and Maintenance Costs of Four Agricultural Tractor Models

Authors: Fatemeh Afsharnia, Mohammad Amin Asoodar, Abbas Abdeshahi

Abstract:

In economical evaluation literature, although the combination of some variables such as repair and maintenance costs and accumulated use hours has been widely considered in determining of optimum life for tractor, no investigation has indicated the influence of failure rate on repair and maintenance costs. In this study, the owners of three hundred tractors, which include Massey Ferguson, John Deere and Universal, were interviewed, from five regions of Khouzestan Province. A regression model was used to predict the tractors annual repair and maintenance costs based on failure rate. Results showed that the maximum percentage of annual repair and maintenance costs occurred in engine parts for MF285, JD3140 and U650 tractors while these costs for tire, ring, ball bearing and operator seat were higher compared to other MF399 tractor systems. According to the results of the regression, the failure rate increase would lead to annual repair and maintenance costs increase for all tractors. But, of all the tractors, repair and maintenance costs of JD3140 tractors extremely affected by the failure rate increase.

Keywords: Failure rate, tractor, annual repair and maintenance costs, regression model, Khouzestan.

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641 Lattice Boltzmann Simulation of Binary Mixture Diffusion Using Modern Graphics Processors

Authors: Mohammad Amin Safi, Mahmud Ashrafizaadeh, Amir Ali Ashrafizaadeh

Abstract:

A highly optimized implementation of binary mixture diffusion with no initial bulk velocity on graphics processors is presented. The lattice Boltzmann model is employed for simulating the binary diffusion of oxygen and nitrogen into each other with different initial concentration distributions. Simulations have been performed using the latest proposed lattice Boltzmann model that satisfies both the indifferentiability principle and the H-theorem for multi-component gas mixtures. Contemporary numerical optimization techniques such as memory alignment and increasing the multiprocessor occupancy are exploited along with some novel optimization strategies to enhance the computational performance on graphics processors using the C for CUDA programming language. Speedup of more than two orders of magnitude over single-core processors is achieved on a variety of Graphical Processing Unit (GPU) devices ranging from conventional graphics cards to advanced, high-end GPUs, while the numerical results are in excellent agreement with the available analytical and numerical data in the literature.

Keywords: Lattice Boltzmann model, Graphical processing unit, Binary mixture diffusion, 2D flow simulations, Optimized algorithm.

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640 Aspect Oriented Software Architecture

Authors: Pradip Peter Dey, Ronald F. Gonzales, Gordon W. Romney, Mohammad Amin, Bhaskar Raj Sinha

Abstract:

Natural language processing systems pose a unique challenge for software architectural design as system complexity has increased continually and systems cannot be easily constructed from loosely coupled modules. Lexical, syntactic, semantic, and pragmatic aspects of linguistic information are tightly coupled in a manner that requires separation of concerns in a special way in design, implementation and maintenance. An aspect oriented software architecture is proposed in this paper after critically reviewing relevant architectural issues. For the purpose of this paper, the syntactic aspect is characterized by an augmented context-free grammar. The semantic aspect is composed of multiple perspectives including denotational, operational, axiomatic and case frame approaches. Case frame semantics matured in India from deep thematic analysis. It is argued that lexical, syntactic, semantic and pragmatic aspects work together in a mutually dependent way and their synergy is best represented in the aspect oriented approach. The software architecture is presented with an augmented Unified Modeling Language.

Keywords: Language engineering, parsing, software design, user experience.

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639 Repair and Maintenance Capability and Facilities Availability for MF 285 Tractor Operators in North of Khouzestan Province

Authors: Fatemeh Afsharnia, Mohammad Amin Asoodar, Abbas Abdeshahi, Afshin Marzban

Abstract:

A repairable mechanical system (as agricultural tractor) is subject to deterioration or repeated failure and needs a repair shops and also operator’s capability for the repair and maintenance operations. Data are based on field visits and interviews with 48MF 285 tractor operators from 14 villages collected in north of Khouzestan province. The results showed that most operators were lack the technical skill to service and repair tractors due to insufficient training, specific education and work experience. Inadequate repair and maintenance facilities, such as workshops, mechanics and spare parts depots cause delays in repair work in the survey areas. Farmers do not keep accurate service records and most of them disregard proper maintenance and service of their tractors, such as changing engine oil without following the manufacturer’s recommendations. Since, Repair and maintenance facilities should be established in village areas to guarantee timely repair in case of breakdowns and to make spare parts available at low price. The operators should keep service records accurately and adhere to maintenance and service schedules according to the manufacturer’s instructions. They should also be encouraged to do the service and maintain their tractors properly.

Keywords: Operators’ capability, Facilities availability, Repair and maintenance, MF 285 tractors.

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638 New Application of EHTA for the Generalized(2+1)-Dimensional Nonlinear Evolution Equations

Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi

Abstract:

In this paper, the generalized (2+1)-dimensional Calogero-Bogoyavlenskii-Schiff (shortly CBS) equations are investigated. We employ the Hirota-s bilinear method to obtain the bilinear form of CBS equations. Then by the idea of extended homoclinic test approach (shortly EHTA), some exact soliton solutions including breather type solutions are presented.

Keywords: EHTA, (2+1)-dimensional CBS equations, (2+1)-dimensional breaking solution equation, Hirota's bilinear form.

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637 Highly Accurate Target Motion Compensation Using Entropy Function Minimization

Authors: Amin Aghatabar Roodbary, Mohammad Hassan Bastani

Abstract:

One of the defects of stepped frequency radar systems is their sensitivity to target motion. In such systems, target motion causes range cell shift, false peaks, Signal to Noise Ratio (SNR) reduction and range profile spreading because of power spectrum interference of each range cell in adjacent range cells which induces distortion in High Resolution Range Profile (HRRP) and disrupt target recognition process. Thus Target Motion Parameters (TMPs) effects compensation should be employed. In this paper, such a method for estimating TMPs (velocity and acceleration) and consequently eliminating or suppressing the unwanted effects on HRRP based on entropy minimization has been proposed. This method is carried out in two major steps: in the first step, a discrete search method has been utilized over the whole acceleration-velocity lattice network, in a specific interval seeking to find a less-accurate minimum point of the entropy function. Then in the second step, a 1-D search over velocity is done in locus of the minimum for several constant acceleration lines, in order to enhance the accuracy of the minimum point found in the first step. The provided simulation results demonstrate the effectiveness of the proposed method.

Keywords: ATR, HRRP, motion compensation, SFW, TMP.

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636 Some Exact Solutions of the (2+1)-Dimensional Breaking Soliton Equation using the Three-wave Method

Authors: Mohammad Taghi Darvishi, Mohammad Najafi

Abstract:

This paper considers the (2+1)-dimensional breaking soliton equation in its bilinear form. Some exact solutions to this equation are explicitly derived by the idea of three-wave solution method with the assistance of Maple. We can see that the new idea is very simple and straightforward.

Keywords: Soliton solution, computerized symbolic computation, painleve analysis, (2+1)-dimensional breaking soliton equation, Hirota's bilinear form.

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635 The Impact of e-Learning and e-Teaching

Authors: Mohammad Mohammad

Abstract:

With the exponential progress of technological development comes a strong sense that events are moving too quickly for our schools and that teachers may be losing control of them in the process. This paper examines the impact of e-learning and e-teaching in universities, from both the student and teacher perspective. In particular, it is shown that e-teachers should focus not only on the technical capacities and functions of IT materials and activities, but must attempt to more fully understand how their e-learners perceive the learning environment. From the e-learner perspective, this paper indicates that simply having IT tools available does not automatically translate into all students becoming effective learners. More evidence-based evaluative research is needed to allow e-learning and e-teaching to reach full potential.

Keywords: e-learning, e-teaching, distance learning, education

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634 Fuzzy Control of a Three Phase ThyristorizedInduction Motor

Authors: Abolfazl Jalilvand, Mohammad Reza Feyzi, Sohrab Khanmohammad, Mohammad Bagher Bana Sharifian, Ali Sajjadi

Abstract:

Nowadays the control of stator voltage at a constant frequency is one of the traditional and low expense methods in order to control the speed of induction motors near its nominal speed. The torque of induction motor is a nonlinear function of the firing angle, phase angle and speed. In this paper the speed control of induction motor regarding various load torque and under different conditions will be investigated based on a fuzzy controller with inverse training.

Keywords: Three phase induction motor, AC converter, speedcontrol, fuzzy control.

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633 Quantitative Analysis of PCA, ICA, LDA and SVM in Face Recognition

Authors: Liton Jude Rozario, Mohammad Reduanul Haque, Md. Ziarul Islam, Mohammad Shorif Uddin

Abstract:

Face recognition is a technique to automatically identify or verify individuals. It receives great attention in identification, authentication, security and many more applications. Diverse methods had been proposed for this purpose and also a lot of comparative studies were performed. However, researchers could not reach unified conclusion. In this paper, we are reporting an extensive quantitative accuracy analysis of four most widely used face recognition algorithms: Principal Component Analysis (PCA), Independent Component Analysis (ICA), Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM) using AT&T, Sheffield and Bangladeshi people face databases under diverse situations such as illumination, alignment and pose variations.

Keywords: PCA, ICA, LDA, SVM, face recognition, noise.

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632 A Review of Control Schemes for Active Power Filters in Order to Power Quality Improvement

Authors: Mohammad Hasan Raouf, Azim Nowbakht, Mohammad Bagher Haddadi, Mohammad Reza Tabatabaei

Abstract:

Power quality has become a very important issue recently due to the impact on electricity suppliers, equipment manufacturers and customers. Power quality is described as the variation of voltage, current and frequency in a power system. Voltage magnitude is one of the major factors that determine the quality of power. Indeed, custom power technology, the low-voltage counterpart of the more widely known flexible ac transmission system (FACTS) technology, aimed at high-voltage power transmission applications, has emerged as a credible solution to solve many problems relating to power quality problems. There are various power quality problems such as voltage sags, swells, flickers, interruptions and harmonics etc. Active Power Filter (APF) is one of the custom power devices and can mitigate harmonics, reactive power and unbalanced load currents originating from load side. In this study, an extensive review of APF studies, the advantages and disadvantages of each introduced methods are presented. The study also helps the researchers to choose the optimum control techniques and power circuit configuration for APF applications.

Keywords: Power Quality, Custom Power, Active Filter, Control Approach.

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631 Stabilization of the Lorenz Chaotic Equations by Fuzzy Controller

Authors: Behrooz Rezaie, Zahra Rahmani Cherati, Mohammad Reza Jahed Motlagh, Mohammad Farrokhi

Abstract:

In this paper, a fuzzy controller is designed for stabilization of the Lorenz chaotic equations. A simple Mamdani inference method is used for this purpose. This method is very simple and applicable for complex chaotic systems and it can be implemented easily. The stability of close loop system is investigated by the Lyapunov stabilization criterion. A Lyapunov function is introduced and the global stability is proven. Finally, the effectiveness of this method is illustrated by simulation results and it is shown that the performance of the system is improved.

Keywords: Chaotic system, Fuzzy control, Lorenz equation.

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630 Comparison of the Existing Methods in Determination of the Characteristic Polynomial

Authors: Mohammad Saleh Tavazoei, Mohammad Haeri

Abstract:

This paper presents comparison among methods of determination of the characteristic polynomial coefficients. First, the resultant systems from the methods are compared based on frequency criteria such as the closed loop bandwidth, gain and phase margins. Then the step responses of the resultant systems are compared on the basis of the transient behavior criteria including overshoot, rise time, settling time and error (via IAE, ITAE, ISE and ITSE integral indices). Also relative stability of the systems is compared together. Finally the best choices in regards to the above diverse criteria are presented.

Keywords: Characteristic Polynomial, Transient Response, Filters, Stability.

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629 Exact Three-wave Solutions for High Nonlinear Form of Benjamin-Bona-Mahony-Burgers Equations

Authors: Mohammad Taghi Darvishi, Maliheh Najafi, Mohammad Najafi

Abstract:

By means of the idea of three-wave method, we obtain some analytic solutions for high nonlinear form of Benjamin-Bona- Mahony-Burgers (shortly BBMB) equations in its bilinear form.

Keywords: Benjamin-Bona-Mahony-Burgers equations, Hirota's bilinear form, three-wave method.

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628 Preparation and Evaluation of New Nanocatalysts for Selective Oxidation of H2S to Sulfur

Authors: Mohammad Rezaee, Mohammad Kazemeini, Ali Morad Rashidi, Moslem Fattahi

Abstract:

Selective oxidation of H2S to elemental sulfur in a fixed bed reactor over newly synthesized alumina nanocatalysts was physio-chemically investigated and results compared with a commercial Claus catalyst. Amongst these new materials, Al2O3- supported sodium oxide prepared with wet chemical technique and Al2O3 nanocatalyst prepared with spray pyrolysis method were the most active catalysts for selective oxidation of H2S to elemental sulfur. Other prepared nanocatalysts were quickly deactivated, mainly due to the interaction with H2S and conversion into sulfides.

Keywords: H2S, Claus process, Al2O3, Spray pyrolysis method, Wet chemical technique

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627 Flood Scenarios for Hydrological and Hydrodynamic Modelling

Authors: Md. Sharif Imam Ibne Amir, Mohammad Masud Kamal khan, Mohammad Golam Rasul, Raj H Sharma, Fatema Akram

Abstract:

Future flood can be predicted using the probable maximum flood (PMF). PMF is calculated using the historical discharge or rainfall data considering the other climatic parameters remaining stationary. However climate is changing globally and the key climatic variables are temperature, evaporation, rainfall and sea level rise are likely to change. To develop scenarios to a basin or catchment scale these important climatic variables should be considered. Nowadays scenario based on climatic variables is more suitable than PMF. Six scenarios were developed for a large Fitzroy basin and presented in this paper.

Keywords: Climate change, rainfall, potential evaporation, scenario, sea level rise (SLR), sub-catchment.

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626 Cyber Warriors for Cyber Security and Information Assurance- An Academic Perspective

Authors: Ronald F. Gonzales, Gordon W. Romney, Pradip Peter Dey, Mohammad Amin, Bhaskar Raj Sinha

Abstract:

A virtualized and virtual approach is presented on academically preparing students to successfully engage at a strategic perspective to understand those concerns and measures that are both structured and not structured in the area of cyber security and information assurance. The Master of Science in Cyber Security and Information Assurance (MSCSIA) is a professional degree for those who endeavor through technical and managerial measures to ensure the security, confidentiality, integrity, authenticity, control, availability and utility of the world-s computing and information systems infrastructure. The National University Cyber Security and Information Assurance program is offered as a Master-s degree. The emphasis of the MSCSIA program uniquely includes hands-on academic instruction using virtual computers. This past year, 2011, the NU facility has become fully operational using system architecture to provide a Virtual Education Laboratory (VEL) accessible to both onsite and online students. The first student cohort completed their MSCSIA training this past March 2, 2012 after fulfilling 12 courses, for a total of 54 units of college credits. The rapid pace scheduling of one course per month is immensely challenging, perpetually changing, and virtually multifaceted. This paper analyses these descriptive terms in consideration of those globalization penetration breaches as present in today-s world of cyber security. In addition, we present current NU practices to mitigate risks.

Keywords: Cyber security, information assurance, mitigate risks, virtual machines, strategic perspective.

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625 Detection of Legionella pneumophila in Cooling Water Systems of Hospitals and Nursing Homes of Kerman City, Iran by Semi- Nested PCR

Authors: Mohammad Ahmadinejad, Mohammad Reza Shakibaie, Kyvan Shams, Mohammad Khalili

Abstract:

Legionella pneumophila is involved in more than 95% cases of severe atypical pneumonia. Infection is mainly by inhalation the indoor aerosols through the water-coolant systems. Because some Legionella strains may be viable but not culturable, therefore, Taq polymerase, DNA amplification and semi-nested-PCR were carried out to detect Legionella-specific 16S-rDNA sequence. For this purpose, 1.5 litter of water samples from 77 water-coolant system were collected from four different hospitals, two nursing homes and one student hostel in Kerman city of Iran, each in a brand new plastic bottle during summer season of 2006 (from April to August). The samples were filtered in the sterile condition through the Millipore Membrane Filter. DNA was extracted from membrane and used for PCR to detect Legionella spp. The PCR product was then subjected to semi-nested PCR for detection of L. pneumophila. Out of 77 water samples that were tested by PCR, 30 (39%) were positive for most species of Legionella. However, L. pneumophila was detected from 14 (18.2%) water samples by semi-nested PCR. From the above results it can be concluded that water coolant systems of different hospitals and nursing homes in Kerman city of Iran are highly contaminated with L. pneumophila spp. and pose serious concern. So, we recommend avoiding such type of coolant system in the hospitals and nursing homes.

Keywords: Legionella pneumophila, water-coolant system, semi-nested -PCR.

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624 Synthesis of Sterile and Pyrogen Free Biogenic Magnetic Nanoparticles: Biotechnological Potential of Magnetotactic Bacteria for Production of Nanomaterials

Authors: Saeid Ghorbanzadeh-Mashkani, Parisa Tajer-Mohammad-Ghazvini, Ahmad Nozad-Golikand, Rouha Kasra-Kermanshahi, Mohammad-Reza Davarpanah

Abstract:

Today, biogenic magnetite nanoparticles among magnetic nanoparticles have unique attracted attention because of their magnetic characteristics and potential applications in various fields such as therapeutic and diagnostic. A well known example of these biogenic nanoparticles is magnetosomes of magnetotactic bacteria. In this research, we used two different types of technique for the isolation and purification of magnetosome nanoparticles from the isolated magnetotactic bacterial cells, heat-alkaline treatment and sonication. Also we evaluated pyrogen content and sterility of synthesized the isolated individual magnetosome by the Limulus Amoebocyte Lysate test and direct impedimetric method respectively.

Keywords: Biogenic magnetic nanoparticles, Magnetosome, Magnetotactic bacteria, Nanobiotechnology

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