Search results for: Protocol Design

Authors: Asmir Gogic, Aljo Mujcic, Sandra Ibric, Nermin Suljanovic

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Ubiquity of natural disasters during last few decades have risen serious questions towards the prediction of such events and human safety. Every disaster regardless its proportion has a precursor which is manifested as a disruption of some environmental parameter such as temperature, humidity, pressure, vibrations and etc. In order to anticipate and monitor those changes, in this paper we propose an overall system for disaster prediction and monitoring, based on wireless sensor network (WSN). Furthermore, we introduce a modified and simplified WSN routing protocol built on the top of the trickle routing algorithm. Routing algorithm was deployed using the bluetooth low energy protocol in order to achieve low power consumption. Performance of the WSN network was analyzed using a real life system implementation. Estimates of the WSN parameters such as battery life time, network size and packet delay are determined. Based on the performance of the WSN network, proposed system can be utilized for disaster monitoring and prediction due to its low power profile and mesh routing feature.

Keywords: Bluetooth low energy, disaster prediction, mesh routing protocols, wireless sensor networks.

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Authors: M. Gooroochurn, M. Ovinis, D. Kerr, K. Bouazza-Marouf, M. Vloeberghs

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A registration framework for image-guided robotic surgery is proposed for three emergency neurosurgical procedures, namely Intracranial Pressure (ICP) Monitoring, External Ventricular Drainage (EVD) and evacuation of a Chronic Subdural Haematoma (CSDH). The registration paradigm uses CT and white light as modalities. This paper presents two simulation studies for a preliminary evaluation of the registration protocol: (1) The loci of the Target Registration Error (TRE) in the patient-s axial, coronal and sagittal views were simulated based on a Fiducial Localisation Error (FLE) of 5 mm and (2) Simulation of the actual framework using projected views from a surface rendered CT model to represent white light images of the patient. Craniofacial features were employed as the registration basis to map the CT space onto the simulated intraoperative space. Photogrammetry experiments on an artificial skull were also performed to benchmark the results obtained from the second simulation. The results of both simulations show that the proposed protocol can provide a 5mm accuracy for these neurosurgical procedures.

Keywords: Image-guided Surgery, Multimodality Registration, Photogrammetry, Preoperative to Intraoperative Registration.

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Authors: T. O. Olwal, K. Djouani, B. J. van Wyk, Y. Hamam, P. Siarry, N. Ntlatlapa

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Multi-Radio Multi-Channel (MRMC) systems are key to power control problems in wireless mesh networks (WMNs). In this paper, we present asynchronous multiple-state based power control for MRMC WMNs. First, WMN is represented as a set of disjoint Unified Channel Graphs (UCGs). Second, each network interface card (NIC) or radio assigned to a unique UCG adjusts transmission power using predicted multiple interaction state variables (IV) across UCGs. Depending on the size of queue loads and intra- and inter-channel states, each NIC optimizes transmission power locally and asynchronously. A new power selection MRMC unification protocol (PMMUP) is proposed that coordinates interactions among radios. The efficacy of the proposed method is investigated through simulations.

Keywords: Asynchronous convergence, Multi-Radio Multi-Channel (MRMC), Power Selection Multi-Radio Multi-Channel Unification Protocol (PMMUP) and Wireless Mesh Networks(WMNs)

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Authors: Ashok Kumar, Narottam Chand, Vinod Kumar

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Due to the limited energy resources, energy efficient operation of sensor node is a key issue in wireless sensor networks. Clustering is an effective method to prolong the lifetime of energy constrained wireless sensor network. However, clustering in wireless sensor network faces several challenges such as selection of an optimal group of sensor nodes as cluster, optimum selection of cluster head, energy balanced optimal strategy for rotating the role of cluster head in a cluster, maintaining intra and inter cluster connectivity and optimal data routing in the network. In this paper, we propose a protocol supporting an energy efficient clustering, cluster head selection/rotation and data routing method to prolong the lifetime of sensor network. Simulation results demonstrate that the proposed protocol prolongs network lifetime due to the use of efficient clustering, cluster head selection/rotation and data routing.

Keywords: Wireless sensor networks, clustering, energy efficient, localization.

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Authors: Abdi T. Abdalla, Justinian Anatory

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This paper investigates the possibility of improving throughputs of some Media Access Controls protocols such as ALOHA, slotted ALOHA and Carrier Sense Multiple Access with Collision Avoidance with the aim of increasing the performance of Powerline access networks. In this investigation, the real Powerline network topology in Tanzania located in Dar es Salaam City, Kariakoo area was used as a case study. During this investigation, Wireshark Network Protocol Analyzer was used to analyze data traffic of similar existing network for projection purpose and then the data were simulated using MATLAB. This paper proposed and analyzed three improvement techniques based on collision domain, packet length and combination of the two. From the results, it was found that the throughput of Carrier Sense Multiple Access with Collision Avoidance protocol improved noticeably while ALOHA and slotted ALOHA showed insignificant changes especially when the hybrid techniques were employed.

Keywords: Access Network, ALOHA, Broadband Powerline Communication, Slotted ALOHA, CSMA/CA and MAC Protocols.

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Authors: Sungju Lee, Jangsoo Lee , Hongjoong Sin, Seunghwan Yoo, Sanghyuck Lee, Jaesik Lee, Yongjun Lee, Sungchun Kim

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The wireless sensor networks have been extensively deployed and researched. One of the major issues in wireless sensor networks is a developing energy-efficient clustering protocol. Clustering algorithm provides an effective way to prolong the lifetime of a wireless sensor networks. In the paper, we compare several clustering protocols which significantly affect a balancing of energy consumption. And we propose an Energy-Efficient Distributed Unequal Clustering (EEDUC) algorithm which provides a new way of creating distributed clusters. In EEDUC, each sensor node sets the waiting time. This waiting time is considered as a function of residual energy, number of neighborhood nodes. EEDUC uses waiting time to distribute cluster heads. We also propose an unequal clustering mechanism to solve the hot-spot problem. Simulation results show that EEDUC distributes the cluster heads, balances the energy consumption well among the cluster heads and increases the network lifetime.

Keywords: Wireless Sensor Network, Distributed UnequalClustering, Multi-hop, Lifetime.

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Authors: Hamed Rajabi, Naser Nematbakhsh, Naser Movahediniya

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IP multicasting is a key technology for many existing and emerging applications on the Internet. Furthermore, with increasing popularity of wireless devices and mobile equipment, it is necessary to determine the best way to provide this service in a wireless environment. IETF Mobile IP, that provides mobility for hosts in IP networks, proposes two approaches for mobile multicasting, namely, remote subscription (MIP-RS) and bi-directional tunneling (MIP-BT). In MIP-RS, a mobile host re-subscribes to the multicast groups each time it moves to a new foreign network. MIP-RS suffers from serious packet losses while mobile host handoff occurs. In MIP-BT, mobile hosts send and receive multicast packets by way of their home agents (HAs), using Mobile IP tunnels. Therefore, it suffers from inefficient routing and wastage of system resources. In this paper, we propose a protocol called Mobile Multicast support using Old Foreign Agent (MMOFA) for Mobile Hosts. MMOFA is derived from MIP-RS and with the assistance of Mobile host's Old foreign agent, routes the missing datagrams due to handoff in adjacent network via tunneling. Also, we studied the performance of the proposed protocol by simulation under ns-2.27. The results demonstrate that MMOFA has optimal routing efficiency and low delivery cost, as compared to other approaches.

Keywords: Mobile Multicast, Mobile IP, MMOFA, NS-2. 27.

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Authors: Ghalem Belalem, Yahya Slimani

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Large scale systems such as computational Grid is a distributed computing infrastructure that can provide globally available network resources. The evolution of information processing systems in Data Grid is characterized by a strong decentralization of data in several fields whose objective is to ensure the availability and the reliability of the data in the reason to provide a fault tolerance and scalability, which cannot be possible only with the use of the techniques of replication. Unfortunately the use of these techniques has a height cost, because it is necessary to maintain consistency between the distributed data. Nevertheless, to agree to live with certain imperfections can improve the performance of the system by improving competition. In this paper, we propose a multi-layer protocol combining the pessimistic and optimistic approaches conceived for the data consistency maintenance in large scale systems. Our approach is based on a hierarchical representation model with tree layers, whose objective is with double vocation, because it initially makes it possible to reduce response times compared to completely pessimistic approach and it the second time to improve the quality of service compared to an optimistic approach.

Keywords: Data Grid, replication, consistency, optimistic approach, pessimistic approach.

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Authors: Adil Loya, Ali Haider, Arslan A. Ghaffor, Abubaker Siddique

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Simulation and modelling of Unmanned Aerial Vehicle (UAV) has gained wide popularity in front of aerospace community. The demand of designing and modelling optimized control system for UAV has increased ten folds since last decade, as next generation warfare is dependent on unmanned technologies. Therefore, this research focuses on the simulation of nonlinear UAV dynamics on Simulink and its integration with Flightgear. There has been lots of research on implementation of optimizing control using Simulink, however, there are fewer known techniques to simulate these dynamics over Flightgear and a tedious technique of acquiring data has been tackled in this research horizon. Sending data to Flightgear is easy but receiving it from Simulink is not that straight forward, i.e. we can only receive control data on the output. However, in this research we have managed to get the data out from the Flightgear by implementation of level 2 s-function block within Simulink. Moreover, the results captured from Flightgear over a Universal Datagram Protocol (UDP) communication are then compared with the attitude signal that were sent previously. This provide useful information regarding the difference in outputs attained from Simulink to Flightgear. It was found that values received on Simulink were in high agreement with that of the Flightgear output. And complete study has been conducted in a discrete way.

Keywords: aerospace, flight control, FlightGear, communication, Simulink

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Authors: Tianchai Suksri, U-thai Sritheeravirojana, Arjin Numsomran, Viriya Kongrattana, Thongchai Werataweemart

Abstract:

A control system design with Characteristic Ratio Assignment (CRA) is proven that effective for SISO control design. But the control system design for MIMO via CRA is not concrete procedure. In this paper presents the control system design method for quadruple-tank process via CRA. By using the decentralized method for both minimum phase and non-minimum phase are made. The results from PI and PID controller design via CRA can be illustrated the validity of our approach by MATLAB.

Keywords: CRA, Quadruple-Tank.

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Authors: B.Gholami, S.Gholami, A.Forouzantabar, Sh.Bazyari

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A new design approach for three-stage operational amplifiers (op-amps) is proposed. It allows to actually implement a symmetrical push-pull class-AB amplifier output stage for wellestablished three-stage amplifiers using a feedforward transconductance stage. Compared with the conventional design practice, the proposed approach leads to a significant improvement of the symmetry between the positive and the negative op-amp step response, resulting in similar values of the positive/negative settling time. The new approach proves to be very useful in order to fully exploit the potentiality allowed by the op-amp in terms of speed performances. Design examples in a commercial 0.35-μm CMOS prove the effectiveness of theproposed strategy.

Keywords: Low-voltage op amp, design , optimum design

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Authors: Andreas Litke, Kostas Zotos, Alexander Chatzigeorgiou, George Stephanides

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The importance of low power consumption is widely acknowledged due to the increasing use of portable devices, which require minimizing the consumption of energy. Energy dissipation is heavily dependent on the software used in the system. Applying design patterns in object-oriented designs is a common practice nowadays. In this paper we analyze six design patterns and explore the effect of them on energy consumption and performance.

Keywords: Design Patterns, Embedded Systems, Energy Consumption, Performance Evaluation, Software Design and Development, Software Engineering.

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Authors: Bin Su

Abstract:

A hotel mainly uses its energy on water heating, space heating, refrigeration, space cooling, cooking, lighting and other building services. A number of 4-5 stars hotels in Auckland city are selected for this study. Comparing with the energy used for others, the energy used for the internal space thermal control (e.g. internal space heating) is more closely related to the hotel building itself. This study not only investigates relationship between annual energy (and winter energy) consumptions and building design data but also relationships between winter extra energy consumption and building design data. This study is to identify the major design factors that significantly impact hotel energy consumption for improving the future hotel design for energy efficient.

Keywords: Hotel building design, building energy, building passive design, energy efficiency.

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Authors: Ghassan A. Abed, Samir I. Badrawi

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The advancement in wireless technology with the wide use of mobile devices have drawn the attention of the research and technological communities towards wireless environments, such as Wireless Local Area Networks (WLANs), Wireless Wide Area Networks (WWANs), and mobile systems and ad-hoc networks. Unfortunately, wired and wireless networks are expressively different in terms of link reliability, bandwidth, and time of propagation delay and by adapting new solutions for these enhanced telecommunications, superior quality, efficiency, and opportunities will be provided where wireless communications were otherwise unfeasible. Some researchers define 4G as a significant improvement of 3G, where current cellular network’s issues will be solved and data transfer will play a more significant role. For others, 4G unifies cellular and wireless local area networks, and introduces new routing techniques, efficient solutions for sharing dedicated frequency bands, and an increased mobility and bandwidth capacity. This paper discusses the possible solutions and enhancements probabilities that proposed to improve the performance of Transmission Control Protocol (TCP) over different wireless networks and also the paper investigated each approach in term of advantages and disadvantages.

Keywords: TCP, Wireless Networks, Cellular Systems, WLAN.

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Authors: Moses F. Oduori, Enoch K. Musyoka, Thomas O. Mbuya

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The selection of materials is an essential task in mechanical design processes. This paper sets out to demonstrate the application of analytical decision making during mechanical design and, particularly, in selecting a suitable material for a given application. Equations for the mechanical design of a manual winch rope drum are used to derive quantitative material performance indicators, which are then used in a multiple attribute decision making (MADM) model to rank the candidate materials. Thus, the processing of mechanical design considerations and material properties data into information that is suitable for use in a quantitative materials selection process is demonstrated for the case of a rope drum design. Moreover, Microsoft Excel^®, a commonly available computer package, is used in the selection process. The results of the materials selection process are in agreement with current industry practice in rope drum design. The procedure that is demonstrated here should be adaptable to other design situations in which a need arises for the selection of engineering materials, and other engineering entities.

Keywords: Design Decisions, Materials Selection, Mechanical Design, Rope Drum Design.

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Authors: C. S. Caires, A. Barbosa, W. Hanyou

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In this paper, we present the main results achieved during a five-week international workshop on Interactive Furniture for the Classroom, with 22 Chinese design students, in Jiangmen city (Guangdong province, China), and five teachers from Portugal, France, Iran, Macao SAR, and China. The main goal was to engage design students from China with new skills and practice methodologies towards interactive design research for furniture and product design for the classroom. The final results demonstrate students' concerns on improving Chinese furniture design for the classrooms, including solutions related to collaborative learning and human-interaction design for interactive furniture products. The findings of the research led students to the fabrication of five original prototypes: two for kindergartens ('Candy' and 'Tilt-tilt'), two for primary schools ('Closer' and 'Eks(x)'), and one for art/creative schools ('Wave'). From the findings, it was also clear that collaboration, personalization, and project-based teaching are still neglected when designing furniture products for the classroom in China. Students focused on these issues and came up with creative solutions that could transform this educational field in China.

Keywords: Product design, interface design, interactive design, collaborative education and design research, design prototyping.

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Authors: Viet-Hung Nguyen, Luh-Maan Chang

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Design should be viewed concurrently by three ways as transformation, flow and value generation. An innovative approach to solve design – related problems is described as the integrated product - process design. As a foundation for a formal framework consisting of organizing principles and techniques, Work Structuring has been developed to guide efforts in the integration that enhances the development of operation and process design in alignment with product design. Vietnam construction projects are facing many delays, and cost overruns caused mostly by design related problems. A better design management that integrates product and process design could resolve these problems. A questionnaire survey and in – depth interviews were used to investigate the feasibility of applying Work Structuring to construction projects in Vietnam. The purpose of this paper is to present the research results and to illustrate the possible problems and potential solutions when Work Structuring is implemented to construction projects in Vietnam.

Keywords: integrated product – process design, Work Structuring, construction projects, Vietnam

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Authors: Carsten Röcker

Abstract:

This paper presents a set of guidelines for the design of multi-user awareness systems. In a first step, general requirements for team awareness systems are analyzed. In the second part of the paper, the identified requirements are aggregated and transformed into concrete design guidelines for the development of team awareness systems.

Keywords: User Interface Design, Awareness Systems, User- Centered Design, Human Factors.

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Authors: Eunil Park, Kwangsu Cho

Abstract:

The wireless link can be unreliable in realistic wireless sensor networks (WSNs). Energy efficient and reliable data forwarding is important because each node has limited resources. Therefore, we must suggest an optimal solution that considers using the information of the node-s characteristics. Previous routing protocols were unsuited to realistic asymmetric WSNs. In this paper, we propose a Protocol that considers Both sides of Link-quality and Energy (PBLE), an optimal routing protocol that balances modified link-quality, distance and energy. Additionally, we propose a node scheduling method. PBLE achieves a longer lifetime than previous routing protocols and is more energy-efficient. PBLE uses energy, local information and both sides of PRR in a 1-hop distance. We explain how to send data packets to the destination node using the node's information. Simulation shows PBLE improves delivery rate and network lifetime compared to previous schemes. Moreover, we show the improvement in various WSN environments.

Keywords: energy-efficient, lifetime, PBLE, unreliable

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Authors: L. Zehtaban, D. Roller

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Apart from geometry, functionality is one of the most significant hallmarks of a product. The functionality of a product can be considered as the fundamental justification for a product existence. Therefore a functional analysis including a complete and reliable descriptor has a high potential to improve product development process in various fields especially in knowledge-based design. One of the important applications of the functional analysis and indexing is in retrieval and design reuse concept. More than 75% of design activity for a new product development contains reusing earlier and existing design know-how. Thus, analysis and categorization of product functions concluded by functional indexing, influences directly in design optimization. This paper elucidates and evaluates major classes for functional analysis by discussing their major methods. Moreover it is finalized by presenting a noble hybrid approach for functional analysis.

Keywords: Functional analysis, design reuse, functionalindexing and representation.

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Authors: Boonorm Chomtee, John J. Borkowski

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The objective of the research is to study and compare response surface designs: Central composite designs (CCD), Box- Behnken designs (BBD), Small composite designs (SCD), Hybrid designs, and Uniform shell designs (USD) over sets of reduced models when the design is in a spherical region for 3 and 4 design variables. The two optimality criteria ( D and G ) are considered which larger values imply a better design. The comparison of design optimality criteria of the response surface designs across the full second order model and sets of reduced models for 3 and 4 factors based on the two criteria are presented.

Keywords: design optimality criteria, reduced models, response surface design, spherical design region

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Authors: Sri Kusumawati Md Daud, Fauzan Mustaffa, Hanafizan Hussain, Md Najib Osman

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Does open ended creative technology give positive impact in learning design? Although there are many researchers had examined on the impact of technology on design education but there are very few conclusive researches done on the impact of open ended used of software to learning design. This paper sought to investigate a group of student-s experience on relatively wider range of software application within the context of design project. A typography design project was used to create a learning environment with the aim of inculcate design skills into the learners and increase their creative problem-solving and critical thinking skills. The methods used in this study were questionnaire survey and personal observation which will be focus on the individual and group response during the completion of the task.

Keywords: Learning Tool, Creative Technology, Software, Software Skills, Typography Design.

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Authors: Kavi K. Khedo, R. K. Subramanian

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Wireless sensor networks (WSN) are currently receiving significant attention due to their unlimited potential. These networks are used for various applications, such as habitat monitoring, automation, agriculture, and security. The efficient nodeenergy utilization is one of important performance factors in wireless sensor networks because sensor nodes operate with limited battery power. In this paper, we proposed the MiSense hierarchical cluster based routing algorithm (MiCRA) to extend the lifetime of sensor networks and to maintain a balanced energy consumption of nodes. MiCRA is an extension of the HEED algorithm with two levels of cluster heads. The performance of the proposed protocol has been examined and evaluated through a simulation study. The simulation results clearly show that MiCRA has a better performance in terms of lifetime than HEED. Indeed, MiCRA our proposed protocol can effectively extend the network lifetime without other critical overheads and performance degradation. It has been noted that there is about 35% of energy saving for MiCRA during the clustering process and 65% energy savings during the routing process compared to the HEED algorithm.

Keywords: Clustering algorithm, energy consumption, hierarchical model, sensor networks.

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Authors: A.Mosavi

Abstract:

A novel methodology has been used to design an evaporator coil of a refrigerant. The methodology used is through a complete Computer Aided Design /Computer Aided Engineering approach, by means of a Computational Fluid Dynamic/Finite Element Analysis model which is executed many times for the thermal-fluid exploration of several designs' configuration by an commercial optimizer. Hence the design is carried out automatically by parallel computations, with an optimization package taking the decisions rather than the design engineer. The engineer instead takes decision regarding the physical settings and initializing of the computational models to employ, the number and the extension of the geometrical parameters of the coil fins and the optimization tools to be employed. The final design of the coil geometry found to be better than the initial design.

Keywords: Multi-objective shape optimization, Heat Transfer, multi-physics structures, modeFRONTIER

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Authors: Ren-Junn Hwang, Chung-Chien Lu, Jain-Shing Wu

Abstract:

Cloud outsource storage is one of important services in cloud computing. Cloud users upload data to cloud servers to reduce the cost of managing data and maintaining hardware and software. To ensure data confidentiality, users can encrypt their files before uploading them to a cloud system. However, retrieving the target file from the encrypted files exactly is difficult for cloud server. This study proposes a protocol for performing multikeyword searches for encrypted cloud data by applying k-nearest neighbor technology. The protocol ranks the relevance scores of encrypted files and keywords, and prevents cloud servers from learning search keywords submitted by a cloud user. To reduce the costs of file transfer communication, the cloud server returns encrypted files in order of relevance. Moreover, when a cloud user inputs an incorrect keyword and the number of wrong alphabet does not exceed a given threshold; the user still can retrieve the target files from cloud server. In addition, the proposed scheme satisfies security requirements for outsourced data storage.

Keywords: Fault-tolerance search, multi-keywords search, outsource storage, ranked search, searchable encryption.

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Authors: A.R. Aswatha, T. Basavaraju, S. Sandeep Kumar

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Timing driven physical design, synthesis, and optimization tools need efficient closed-form delay models for estimating the delay associated with each net in an integrated circuit (IC) design. The total number of nets in a modern IC design has increased dramatically and exceeded millions. Therefore efficient modeling of interconnection is needed for high speed IC-s. This paper presents closed–form expressions for RC and RLC interconnection trees in current mode signaling, which can be implemented in VLSI design tool. These analytical model expressions can be used for accurate calculation of delay after the design clock tree has been laid out and the design is fully routed. Evaluation of these analytical models is several orders of magnitude faster than simulation using SPICE.

Keywords: IC design, RC/RLC Interconnection, VLSI Systems.

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Authors: Ram Kumar Soni, Alok Jain, Rajiv Saxena

Abstract:

The tree structured approach of non-uniform filterbank (NUFB) is normally used in perfect reconstruction (PR). The PR is not always feasible due to certain limitations, i.e, constraints in selecting design parameters, design complexity and some times output is severely affected by aliasing error if necessary and sufficient conditions of PR is not satisfied perfectly. Therefore, there has been generalized interest of researchers to go for near perfect reconstruction (NPR). In this proposed work, an optimized tree structure technique is used for the design of NPR non-uniform filterbank. Window functions of Blackman family are used to design the prototype FIR filter. A single variable linear optimization is used to minimize the amplitude distortion. The main feature of the proposed design is its simplicity with linear phase property.

Keywords: Tree structure, NUFB, QMF, NPR.

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Authors: Virginia Melnyk

Abstract:

This paper explores the design for catenary structure using knitted textiles. Using the advantages of Grasshopper and Kangaroo parametric software to simulate and pre-design an overall form, the design is then translated to a pattern that can be made with hand manipulated stitches on a knitting machine. The textile takes advantage of the structure of knitted materials and the ability for it to stretch. Using different types of stitches to control the amount of stretch that can occur in portions of the textile generates an overall formal design. The textile is then hardened in an upside-down hanging position and then flipped right-side-up. This then becomes a structural catenary form. The resulting design is used as a small Cat House for a cat to sit inside and climb on top of.

Keywords: Architectural materials, catenary structures, knitting fabrication, textile design.

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Authors: Zulkeflee Kusin, Mohamad Shanudin Zakaria

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Hierarchical Mobile IPv6 (HMIPv6) was designed to support IP micro-mobility management in the Next Generation Networks (NGN) framework. The main design behind this protocol is the usage of Mobility Anchor Point (MAP) located at any level router of network to support hierarchical mobility management. However, the distance MAP selection in HMIPv6 causes MAP overloaded and increase frequent binding update as the network grows. Therefore, to address the issue in designing MAP selection scheme, we propose a dynamic load control mechanism integrates with a speed detection mechanism (DMS-DLC). From the experimental results we obtain that the proposed scheme gives better distribution in MAP load and increase handover speed.

Keywords: Dynamic load control, HMIPv6, Mobility AnchorPoint, MAP selection scheme

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Authors: Srinivas Bathini, Duraichelvan Raju, Simona Badilescu, Muthukumaran Packirisamy

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A bio-sensing method, based on the plasmonic property of gold nano-islands, has been developed for detection of exosomes in a clinical setting. The position of the gold plasmon band in the UV-Visible spectrum depends on the size and shape of gold nanoparticles as well as on the surrounding environment. By adsorbing various chemical entities, or binding them, the gold plasmon band will shift toward longer wavelengths and the shift is proportional to the concentration. Exosomes transport cargoes of molecules and genetic materials to proximal and distal cells. Presently, the standard method for their isolation and quantification from body fluids is by ultracentrifugation, not a practical method to be implemented in a clinical setting. Thus, a versatile and cutting-edge platform is required to selectively detect and isolate exosomes for further analysis at clinical level. The new sensing protocol, instead of antibodies, makes use of a specially synthesized polypeptide (Vn96), to capture and quantify the exosomes from different media, by binding the heat shock proteins from exosomes. The protocol has been established and optimized by using a glass substrate, in order to facilitate the next stage, namely the transfer of the protocol to a microfluidic environment. After each step of the protocol, the UV-Vis spectrum was recorded and the position of gold Localized Surface Plasmon Resonance (LSPR) band was measured. The sensing process was modelled, taking into account the characteristics of the nano-island structure, prepared by thermal convection and annealing. The optimal molar ratios of the most important chemical entities, involved in the detection of exosomes were calculated as well. Indeed, it was found that the results of the sensing process depend on the two major steps: the molar ratios of streptavidin to biotin-PEG-Vn96 and, the final step, the capture of exosomes by the biotin-PEG-Vn96 complex. The microfluidic device designed for sensing of exosomes consists of a glass substrate, sealed by a PDMS layer that contains the channel and a collecting chamber. In the device, the solutions of linker, cross-linker, etc., are pumped over the gold nano-islands and an Ocean Optics spectrometer is used to measure the position of the Au plasmon band at each step of the sensing. The experiments have shown that the shift of the Au LSPR band is proportional to the concentration of exosomes and, thereby, exosomes can be accurately quantified. An important advantage of the method is the ability to discriminate between exosomes having different origins.

Keywords: Exosomes, gold nano-islands, microfluidics, plasmonic biosensing.

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