Search results for: data validation

Authors: Myungraee Cha, Jun Seok Kim, Seung Hwan Park, Jun-Geol Baek

Abstract:

In manufacturing industries, development of measurement leads to increase the number of monitoring variables and eventually the importance of multivariate control comes to the fore. Statistical process control (SPC) is one of the most widely used as multivariate control chart. Nevertheless, SPC is restricted to apply in processes because its assumption of data as following specific distribution. Unfortunately, process data are composed by the mixture of several processes and it is hard to estimate as one certain distribution. To alternative conventional SPC, therefore, nonparametric control chart come into the picture because of the strength of nonparametric control chart, the absence of parameter estimation. SVDD based control chart is one of the nonparametric control charts having the advantage of flexible control boundary. However,basic concept of SVDD has been an oversight to the important of data characteristic, density distribution. Therefore, we proposed DW-SVDD (Density Weighted SVDD) to cover up the weakness of conventional SVDD. DW-SVDD makes a new attempt to consider dense of data as introducing the notion of density Weight. We extend as control chart using new proposed SVDD and a simulation study of various distributional data is conducted to demonstrate the improvement of performance.

Keywords: Density estimation, Multivariate control chart, Oneclass classification, Support vector data description (SVDD)

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Authors: Hafizullah A. Hashim, Aniza. A

Abstract:

The Spiral development model has been used successfully in many commercial systems and in a good number of defense systems. This is due to the fact that cost-effective incremental commitment of funds, via an analogy of the spiral model to stud poker and also can be used to develop hardware or integrate software, hardware, and systems. To support adaptive, semantic collaboration between domain experts and knowledge engineers, a new knowledge engineering process, called Spiral_OWL is proposed. This model is based on the idea of iterative refinement, annotation and structuring of knowledge base. The Spiral_OWL model is generated base on spiral model and knowledge engineering methodology. A central paradigm for Spiral_OWL model is the concentration on risk-driven determination of knowledge engineering process. The collaboration aspect comes into play during knowledge acquisition and knowledge validation phase. Design rationales for the Spiral_OWL model are to be easy-to-implement, well-organized, and iterative development cycle as an expanding spiral.

Keywords: Domain Expert, Knowledge Base, Ontology, Software Process.

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Authors: Kyoung-Mi Lee, Youn-Mi Lee

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This paper proposes a way to track persons by making use of multiple non-overlapping cameras. Tracking persons on multiple non-overlapping cameras enables data communication among cameras through the network connection between a camera and a computer, while at the same time transferring human feature data captured by a camera to another camera that is connected via the network. To track persons with a camera and send the tracking data to another camera, the proposed system uses a hierarchical human model that comprises a head, a torso, and legs. The feature data of the person being modeled are transferred to the server, after which the server sends the feature data of the human model to the cameras connected over the network. This enables a camera that captures a person's movement entering its vision to keep tracking the recognized person with the use of the feature data transferred from the server.

Keywords: Person tracking, human model, networked cameras, vision-based surveillance.

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Authors: V. Hernandez-Perez, M. Abdulkadir, B. J. Azzopardi

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In this work, new experimental data for slugging frequency in inclined gas-liquid flow are reported, and a new correlation is proposed. Scale experiments were carried out using a mixture of air and water in a 6 m long pipe. Two different pipe diameters were used, namely, 38 and 67 mm. The data were taken with capacitance type sensors at a data acquisition frequency of 200 Hz over an interval of 60 seconds. For the range of flow conditions studied, the liquid superficial velocity is observed to influence the frequency strongly. A comparison of the present data with correlations available in the literature reveals a lack of agreement. A new correlation for slug frequency has been proposed for the inclined flow, which represents the main contribution of this work.

Keywords: slug frequency, inclined flow

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Authors: Yu-Kyung Kang, Suk-Hyung Hwang, Kyoung-Mo Yang

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The tagging data of (users, tags and resources) constitutes a folksonomy that is the user-driven and bottom-up approach to organizing and classifying information on the Web. Tagging data stored in the folksonomy include a lot of very useful information and knowledge. However, appropriate approach for analyzing tagging data and discovering hidden knowledge from them still remains one of the main problems on the folksonomy mining researches. In this paper, we have proposed a folksonomy data mining approach based on FCA for discovering hidden knowledge easily from folksonomy. Also we have demonstrated how our proposed approach can be applied in the collaborative tagging system through our experiment. Our proposed approach can be applied to some interesting areas such as social network analysis, semantic web mining and so on.

Keywords: Folksonomy data mining, formal concept analysis, collaborative tagging, conceptual knowledge discovery, classification.

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Authors: Nazaket Gazieva

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Voice biometric data associated with physiological, psychological and other factors are widely used in forensic phonoscopy. There are various methods for identifying and verifying a person by voice. This article explores the minimum speech signal data as individual parameters of a speech signal. Monozygotic twins are believed to be genetically identical. Using the minimum data of the speech signal, we came to the conclusion that the voice imprint of monozygotic twins is individual. According to the conclusion of the experiment, we can conclude that the minimum indicators of the speech signal are more stable and reliable for phonoscopic examinations.

Keywords: Biometric voice prints, fundamental frequency, phonogram, speech signal, temporal characteristics.

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Authors: Rosida Ab. Razak, Mohamad Shanudin Zakaria

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The fundamental issues in ICT Governance (ICTG) implementation for Malaysian Public Sector (MPS) is how ICT be applied to support improvements in productivity, management effectiveness and the quality of services offered to its citizens. Our main concern is to develop and adopt a common definition and framework to illustrate how ICTG can be used to better align ICT with government’s operations and strategic focus. In particular, we want to identify and categorize factors that drive a successful ICTG process. This paper presents the results of an exploratory study to identify, validate and refine such Critical Success Factors (CSFs) and confirmed seven CSFs and nineteen sub-factors as influential factors that fit MPS after further validated and refined. The Delphi method applied in validation and refining process before being endorsed as appropriate for MPS. The identified CSFs reflect the focus areas that need to be considered strategically to strengthen ICT Governance implementation and ensure business success.

Keywords: IT Governance, Critical Success Factors.

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Authors: Kitti Koonsanit, Chuleerat Jaruskulchai, Poonsak Miphokasap, Apisit Eiumnoh

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In the end of the day, meteorological data and environmental data becomes widely used such as plant varieties selection system. Variety plant selection for planted area is of almost importance for all crops, including varieties of sugarcane. Since sugarcane have many varieties. Variety plant non selection for planting may not be adapted to the climate or soil conditions for planted area. Poor growth, bloom drop, poor fruit, and low price are to be from varieties which were not recommended for those planted area. This paper presents plant varieties selection system for planted areas in Thailand from meteorological data and environmental data by the use of decision tree techniques. With this software developed as an environmental data analysis tool, it can analyze resulting easier and faster. Our software is a front end of WEKA that provides fundamental data mining functions such as classify, clustering, and analysis functions. It also supports pre-processing, analysis, and decision tree output with exporting result. After that, our software can export and display data result to Google maps API in order to display result and plot plant icons effectively.

Keywords: Plant varieties selection system, decision tree, expert recommendation.

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Authors: Tsunwai Gary Yip

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Phase locked loops for data links operating at 10 Gb/s or faster are low phase noise devices designed to operate with a low jitter reference clock. Characterization of their jitter transfer function is difficult because the intrinsic noise of the device is comparable to the random noise level in the reference clock signal. A linear model is proposed to account for the intrinsic noise of a PLL. The intrinsic noise data of a PLL for 10 Gb/s links is presented. The jitter transfer function of a PLL in a test chip for 12.8 Gb/s data links was determined in experiments using the 400 MHz reference clock as the source of simultaneous excitations over a wide range of frequency. The result shows that the PLL jitter transfer function can be approximated by a second order linear model.

Keywords: Intrinsic phase noise, jitter in data link, PLL jitter transfer function, high speed clocking in electronic circuit

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Authors: Saher Ali Al-Sabbah, See Ching Mey, Ong Saw Lan

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This article addresses the procedures to validate the Arabic version of Multiple Intelligence Development Assessment Scale (MIDAS). The content validity was examined based on the experts- judgments on the MIDAS-s items in the Arabic version. The content of eleven items in the Arabic version of MIDAS was modified to match the Arabic context. Then a translation from original English version of MIDAS into Arabic language was performed. The reliability of the Arabic MIDAS was calculated based on test and retest method and found to be 0.85 for the overall MIDAS and for the different subscales ranging between 0.78 - 0.87. The examination of construct validity for the overall Arabic MIDAS and its subscales was established by using Winsteps program version 6 based on Rasch model in order to fit the items into the Arabic context. The findings indicated that, the eight subscales in Arabic version of MIDAS scale have a unidimensionality, and the total number of kept items in the overall scale is 108 items.

Keywords: Rasch-Model, validation, multiple intelligence, and MIDAS scale.

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Authors: AbdulLateef A. Olanrewaju, Mohd F. Khamidi, Arazi Idrus

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This paper is part of an ongoing research on the development of systemic maintenance management model Malaysian university buildings. In order to achieve this aim, there is a need to develop a performance model against which services are measure. Measuring performance is a significant part of maintenance management service delivery. Maintenance organization needs to know where they are in order to provide user-driven services and to enhance productivity. The aim of this paper is to formulate a template or model for university maintenance organization in Malaysia. The model is based on literature review and survey questionnaire and has been validated. Through grounded theory, this paper developed a 8 points matrix for the university maintenance organizations for measuring and improving their service delivery. The potential of the model is guide and assists towards providing value added service delivery through initiating maintenance according to user value system rather than on the condition of the building.

Keywords: Performance matrix, university buildings, users, maintenance organization

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Authors: Chen May Oh, Nadzrah Abu Bakar

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This case study investigates the areas of self-reflection through the written content of four university students’ blogs. The study was undertaken to explore the categories of self-reflection in relation to the use of blogs. Data collection methods included downloading students’ blog entries and recording individual interviews to further support the data. Data was analyzed using computer assisted qualitative data analysis software, Nvivo, to categories and code the data. The categories of self-reflection revealed in the findings showed that university students used blogs to reflect on (1) life in varsity, (2) emotions and feelings, (3) various relationships, (4) personal growth, (5) spirituality, (6) health conditions, (7) busyness with daily chores, (8) gifts for people and themselves and (9) personal interests. Overall, all four of the students had positive experiences and felt satisfied using blogs for self-reflection.

Keywords: Blogging, personal growth, self-reflection, university students.

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Authors: Pejman Hosseinioun, Hasan Shakeri, Ghasem Ghorbanirostam

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In recent years, we have seen an increasing importance of research and study on knowledge source, decision support systems, data mining and procedure of knowledge discovery in data bases and it is considered that each of these aspects affects the others. In this article, we have merged information source and knowledge source to suggest a knowledge based system within limits of management based on storing and restoring of knowledge to manage information and improve decision making and resources. In this article, we have used method of data mining and Apriori algorithm in procedure of knowledge discovery one of the problems of Apriori algorithm is that, a user should specify the minimum threshold for supporting the regularity. Imagine that a user wants to apply Apriori algorithm for a database with millions of transactions. Definitely, the user does not have necessary knowledge of all existing transactions in that database, and therefore cannot specify a suitable threshold. Our purpose in this article is to improve Apriori algorithm. To achieve our goal, we tried using fuzzy logic to put data in different clusters before applying the Apriori algorithm for existing data in the database and we also try to suggest the most suitable threshold to the user automatically.

Keywords: Decision support system, data mining, knowledge discovery, data discovery, fuzzy logic.

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Authors: Moth'd Belal. Al-Daoud

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Clustering is a very well known technique in data mining. One of the most widely used clustering techniques is the k-means algorithm. Solutions obtained from this technique are dependent on the initialization of cluster centers. In this article we propose a new algorithm to initialize the clusters. The proposed algorithm is based on finding a set of medians extracted from a dimension with maximum variance. The algorithm has been applied to different data sets and good results are obtained.

Keywords: clustering, k-means, data mining.

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Authors: Kittisak Kerdprasop, Nittaya Kerdprasop

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Frequent pattern discovery over data stream is a hard problem because a continuously generated nature of stream does not allow a revisit on each data element. Furthermore, pattern discovery process must be fast to produce timely results. Based on these requirements, we propose an approximate approach to tackle the problem of discovering frequent patterns over continuous stream. Our approximation algorithm is intended to be applied to process a stream prior to the pattern discovery process. The results of approximate frequent pattern discovery have been reported in the paper.

Keywords: Frequent pattern discovery, Approximate algorithm, Data stream analysis.

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Authors: Zhou Yu, Jiang Feng

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An adaptive Chinese hand-talking system is presented in this paper. By analyzing the 3 data collecting strategies for new users, the adaptation framework including supervised and unsupervised adaptation methods is proposed. For supervised adaptation, affinity propagation (AP) is used to extract exemplar subsets, and enhanced maximum a posteriori / vector field smoothing (eMAP/VFS) is proposed to pool the adaptation data among different models. For unsupervised adaptation, polynomial segment models (PSMs) are used to help hidden Markov models (HMMs) to accurately label the unlabeled data, then the "labeled" data together with signerindependent models are inputted to MAP algorithm to generate signer-adapted models. Experimental results show that the proposed framework can execute both supervised adaptation with small amount of labeled data and unsupervised adaptation with large amount of unlabeled data to tailor the original models, and both achieve improvements on the performance of recognition rate.

Keywords: sign language recognition, signer adaptation, eMAP/VFS, polynomial segment model.

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Authors: P. Kumsawat, N. Pimpru, K. Attakitmongcol, A.Srikaew

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In this paper, we proposed an efficient data compression strategy exploiting the multi-resolution characteristic of the wavelet transform. We have developed a sensor node called “Smart Sensor Node; SSN". The main goals of the SSN design are lightweight, minimal power consumption, modular design and robust circuitry. The SSN is made up of four basic components which are a sensing unit, a processing unit, a transceiver unit and a power unit. FiOStd evaluation board is chosen as the main controller of the SSN for its low costs and high performance. The software coding of the implementation was done using Simulink model and MATLAB programming language. The experimental results show that the proposed data compression technique yields recover signal with good quality. This technique can be applied to compress the collected data to reduce the data communication as well as the energy consumption of the sensor and so the lifetime of sensor node can be extended.

Keywords: Wireless sensor network, wavelet transform, data compression, ZigBee, skipped high-pass sub-band.

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Authors: R.Mohamad Idris, A.Khairuddin, M.W.Mustafa

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In this paper, a novel method using Bees Algorithm is proposed to determine the optimal allocation of FACTS devices for maximizing the Available Transfer Capability (ATC) of power transactions between source and sink areas in the deregulated power system. The algorithm simultaneously searches the FACTS location, FACTS parameters and FACTS types. Two types of FACTS are simulated in this study namely Thyristor Controlled Series Compensator (TCSC) and Static Var Compensator (SVC). A Repeated Power Flow with FACTS devices including ATC is used to evaluate the feasible ATC value within real and reactive power generation limits, line thermal limits, voltage limits and FACTS operation limits. An IEEE30 bus system is used to demonstrate the effectiveness of the algorithm as an optimization tool to enhance ATC. A Genetic Algorithm technique is used for validation purposes. The results clearly indicate that the introduction of FACTS devices in a right combination of location and parameters could enhance ATC and Bees Algorithm can be efficiently used for this kind of nonlinear integer optimization.

Keywords: ATC, Bees Algorithm, TCSC, SVC

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Authors: Uzma Bashir, Jamaludin Md. Ali

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This study is concerned with the visualization of monotone data using a piecewise C1 rational trigonometric interpolating scheme. Four positive shape parameters are incorporated in the structure of rational trigonometric spline. Conditions on two of these parameters are derived to attain the monotonicity of monotone data and othertwo are leftfree. Figures are used widely to exhibit that the proposed scheme produces graphically smooth monotone curves.

Keywords: Trigonometric splines, Monotone data, Shape preserving, C1 monotone interpolant.

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Authors: Victoriano Valencia García, Luis Usero Aragonés, Eugenio J. Fernández Vicente

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Nowadays, the successful implementation of ICTs is vital for almost any kind of organization. Good governance and ICT management are essential for delivering value, managing technological risks, managing resources and performance measurement. In addition, outsourcing is a strategic IT service solution which complements IT services provided internally in organizations. This paper proposes the measurement tools of a new holistic maturity model based on standards ISO/IEC 20000 and ISO/IEC 38500, and the frameworks and best practices of ITIL and COBIT, with a specific focus on IT outsourcing. These measurement tools allow independent validation and practical application in the field of higher education, using a questionnaire, metrics tables, and continuous improvement plan tables as part of the measurement process. Guidelines and standards are proposed in the model for facilitating adaptation to universities and achieving excellence in the outsourcing of IT services.

Keywords: IT Governance, IT Management, IT Services, Maturity Model, Measurement Tools, Outsourcing.

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Authors: Pham Van Nha, Le Cam Binh

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Particle swarm optimization (PSO) is a population-based stochastic optimization algorithm. PSO was inspired by the natural behavior of birds and fish in migration and foraging for food. PSO is considered as a multidisciplinary optimization model that can be applied in various optimization problems. PSO’s ideas are simple and easy to understand but PSO is only applied in simple model problems. We think that in order to expand the applicability of PSO in complex problems, PSO should be described more explicitly in the form of a mathematical model. In this paper, we represent PSO in a mathematical model and apply in the multivariate data classification. First, PSOs general mathematical model (MPSO) is analyzed as a universal optimization model. Then, Model of Optimal Centroids (MOC) is proposed for the multivariate data classification. Experiments were conducted on some benchmark data sets to prove the effectiveness of MOC compared with several proposed schemes.

Keywords: Analysis of optimization, artificial intelligence-based optimization, optimization for learning and data analysis, global optimization.

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Authors: G. A. Khalid, M. D. Jones, R. Prabhu, A. Mason-Jones, W. Whittington, H. Bakhtiarydavijani, P. S. Theobald

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Head injury in childhood is a common cause of death or permanent disability from injury. However, despite its frequency and significance, there is little understanding of how a child’s head responds during injurious loading. Whilst Infant Post Mortem Human Subject (PMHS) experimentation is a logical approach to understand injury biomechanics, it is the authors’ opinion that a lack of subject availability is hindering potential progress. Computer modelling adds great value when considering adult populations; however, its potential remains largely untapped for infant surrogates. The complexities of child growth and development, which result in age dependent changes in anatomy, geometry and physical response characteristics, present new challenges for computational simulation. Further geometric challenges are presented by the intricate infant cranial bones, which are separated by sutures and fontanelles and demonstrate a visible fibre orientation. This study presents an FE model of a newborn infant’s head, developed from high-resolution computer tomography scans, informed by published tissue material properties. To mimic the fibre orientation of immature cranial bone, anisotropic properties were applied to the FE cranial bone model, with elastic moduli representing the bone response both parallel and perpendicular to the fibre orientation. Biofiedility of the computational model was confirmed by global validation against published PMHS data, by replicating experimental impact tests with a series of computational simulations, in terms of head kinematic responses. Numerical results confirm that the FE head model’s mechanical response is in favourable agreement with the PMHS drop test results.

Keywords: Finite element analysis, impact simulation, infant head trauma, material properties, post mortem human subjects.

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Authors: Ginhsuan Li, Chiuyun Hung, Desheng Chen, Yiwen Wang

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Application-Specific Instruction (ASI ) set Processors (ASIP) have become an important design choice for embedded systems due to runtime flexibility, which cannot be provided by custom ASIC solutions. One major bottleneck in maximizing ASIP performance is the limitation on the data bandwidth between the General Purpose Register File (GPRF) and ASIs. This paper presents the Implicit Registers (IRs) to provide the desirable data bandwidth. An ASI Input/Output model is proposed to formulate the overheads of the additional data transfer between the GPRF and IRs, therefore, an IRs allocation algorithm is used to achieve the better performance by minimizing the number of extra data transfer instructions. The experiment results show an up to 3.33x speedup compared to the results without using IRs.

Keywords: Application-Specific Instruction-set Processors, data bandwidth, configurable processor, implicit register.

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Authors: Hiroyuki Ohsaki, Makoto Imase

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In Grid computing, a data transfer protocol called GridFTP has been widely used for efficiently transferring a large volume of data. Currently, two versions of GridFTP protocols, GridFTP version 1 (GridFTP v1) and GridFTP version 2 (GridFTP v2), have been proposed in the GGF. GridFTP v2 supports several advanced features such as data streaming, dynamic resource allocation, and checksum transfer, by defining a transfer mode called X-block mode. However, in the literature, effectiveness of GridFTP v2 has not been fully investigated. In this paper, we therefore quantitatively evaluate performance of GridFTP v1 and GridFTP v2 using mathematical analysis and simulation experiments. We reveal the performance limitation of GridFTP v1, and quantitatively show effectiveness of GridFTP v2. Through several numerical examples, we show that by utilizing the data streaming feature, the average file transfer time of GridFTP v2 is significantly smaller than that of GridFTP v1.

Keywords: Grid Computing, GridFTP, Performance Evaluation, Queuing Theory.

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Authors: K. Indra Gandhi

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Wireless Sensor Networks (WSNs) have always been characterized for application-specific sensing, relaying and collection of information for further analysis. However, software development was not considered as a separate entity in this process of data collection which has posed severe limitations on the software development for WSN. Software development for WSN is a complex process since the components involved are data-driven, network-driven and application-driven in nature. This implies that there is a tremendous need for the separation of concern from the software development perspective. A layered approach for developing data acquisition design based on Model Driven Development (MDD) has been proposed as the sensed data collection process itself varies depending upon the application taken into consideration. This work focuses on the layered view of the data acquisition process so as to ease the software point of development. A metamodel has been proposed that enables reusability and realization of the software development as an adaptable component for WSN systems. Further, observing users perception indicates that proposed model helps in improving the programmer's productivity by realizing the collaborative system involved.

Keywords: Model-driven development, wireless sensor networks, data acquisition, separation of concern, layered design.

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Authors: Aymen Laadhari, Gábor Székely

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This paper is concerned with the development of a fully implicit and purely Eulerian fluid-structure interaction method tailored for the modeling of the large deformations of elastic membranes in a surrounding Newtonian fluid. We consider a simplified model for the mechanical properties of the membrane, in which the surface strain energy depends on the membrane stretching. The fully Eulerian description is based on the advection of a modified surface tension tensor, and the deformations of the membrane are tracked using a level set strategy. The resulting nonlinear problem is solved by a Newton-Raphson method, featuring a quadratic convergence behavior. A monolithic solver is implemented, and we report several numerical experiments aimed at model validation and illustrating the accuracy of the presented method. We show that stability is maintained for significantly larger time steps.

Keywords: Fluid-membrane interaction, stretching, Eulerian, finite element method, Newton, implicit.

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Authors: Roza Dastres, Mohsen Soori

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In order to electronically exchange information between network users in the web of data, different software such as outlook is presented. So, the traffic of users on a site or even the floors of a building can be decreased as a result of applying a secure and reliable data sharing software. It is essential to provide a fast, secure and reliable network system in the data sharing webs to create an advanced communication systems in the users of network. In the present research work, different encoding methods and algorithms in data sharing systems is studied in order to increase security of data sharing systems by preventing the access of hackers to the transferred data. To increase security in the networks, the possibility of textual conversation between customers of a local network is studied. Application of the encryption and decryption algorithms is studied in order to increase security in networks by preventing hackers from infiltrating. As a result, a reliable and secure communication system between members of a network can be provided by preventing additional traffic in the website environment in order to increase speed, accuracy and security in the network and web systems of data sharing.

Keywords: Secure Socket Layer, Security of networks.

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Authors: Abeer K. Jameel, Harry Evdorides

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Iraq is a middle income country where the road safety issue is considered one of the leading causes of deaths. To control the road risk issue, the Iraqi Ministry of Planning, General Statistical Organization started to organise a collection system of traffic accidents data with details related to their causes and severity. These data are published as an annual report. In this paper, a review of the available crash data in Iraq will be presented. The available data represent the rate of accidents in aggregated level and classified according to their types, road users’ details, and crash severity, type of vehicles, causes and number of causalities. The review is according to the types of models used in road safety studies and research, and according to the required road safety data in the road constructions tasks. The available data are also compared with the road safety dataset published in the United Kingdom as an example of developed country. It is concluded that the data in Iraq are suitable for descriptive and exploratory models, aggregated level comparison analysis, and evaluation and monitoring the progress of the overall traffic safety performance. However, important traffic safety studies require disaggregated level of data and details related to the factors of the likelihood of traffic crashes. Some studies require spatial geographic details such as the location of the accidents which is essential in ranking the roads according to their level of safety, and name the most dangerous roads in Iraq which requires tactic plan to control this issue. Global Road safety agencies interested in solve this problem in low and middle-income countries have designed road safety assessment methodologies which are basing on the road attributes data only. Therefore, in this research it is recommended to use one of these methodologies.

Keywords: Data availability, Iraq, road safety.

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Authors: Yazhi Huang, Jing Yang, Dingge Ying, Yan Zhang, Vorasuk Shotelersuk, Nattiya Hirankarn, Pak Chung Sham, Yu Lung Lau, Wanling Yang

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Human leukocyte antigen (HLA) typing from next generation sequencing (NGS) data has the potential for applications in clinical laboratories and population genetic studies. Here we introduce a novel technique for HLA typing from NGS data based on read-mapping using a comprehensive reference panel containing all known HLA alleles and de novo assembly of the gene-specific short reads. An accurate HLA typing at high-digit resolution was achieved when it was tested on publicly available NGS data, outperforming other newly-developed tools such as HLAminer and PHLAT.

Keywords: Human leukocyte antigens, next generation sequencing, whole exome sequencing, HLA typing.

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Authors: Anton A. Larenkov, Alesya Ya Maruk

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In the present study, highly effective iTLC single strip method for the determination of radiochemical purity (RCP) of ⁶⁸Ga-BCA-peptides was developed (with no double-developing, changing of eluents or other additional manipulation). In this method iTLC-SG strips and commonly used eluent TFA_aq. (3-5 % (v/v)) are used. The method allows determining each of the key radiochemical forms of ⁶⁸Ga (colloidal, bound, ionic) separately with the peaks separation being no less than 4 σ. Rf = 0.0-0.1 for ⁶⁸Ga-colloid; Rf = 0.5-0.6 for ⁶⁸Ga-BCA-peptides; Rf = 0.9-1.0 for ionic ⁶⁸Ga. The method is simple and fast: For developing length of 75 mm only 4-6 min is required (versus 18-20 min for pharmacopoeial method). The method has been tested on various compounds (including ⁶⁸Ga-DOTA-TOC, ⁶⁸Ga-DOTA-TATE, ⁶⁸Ga-NODAGA-RGD₂ etc.). The cross-validation work for every specific form of ⁶⁸Ga showed good correlation between method developed and control (pharmacopoeial) methods. The method can become convenient and much more informative replacement for pharmacopoeial methods, including HPLC.

Keywords: DOTA-TATE, 68Ga, quality control, radiochemical purity, radiopharmaceuticals, iTLC.

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