Search results for: Active cyber defense
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1099

Search results for: Active cyber defense

559 Teaching Project of Architecture in Portugal: Future Perspectives and the Revolution of New Generations

Authors: Patricia Diogo

Abstract:

As teachers and researchers, we often find ourselves grappling with a central question about what it truly means to teach project in architecture in Portugal in the 21st century. It is a question that transcends the simple transmission of technical knowledge or even the sharing of our personal experiences. Rather, it is about developing a teaching pedagogy that responds to and reflects the profound social, environmental, and technological shifts we are currently navigating. Teaching architecture in Portugal today is not a mere continuation of tradition; it is, in essence, a living laboratory of innovation. Each new generation of students enters our classrooms with a hunger not just for design techniques, but for meaning—seeking to understand the role they can play in reshaping our physical and cultural environments. They are not passive recipients of knowledge, but active participants in the creation of a more sustainable, ethical, and thoughtful architecture.

Keywords: Architecture, heritage, memories, project studio, teaching.

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558 Realtime Lip Contour Tracking For Audio-Visual Speech Recognition Applications

Authors: Mehran Yazdi, Mehdi Seyfi, Amirhossein Rafati, Meghdad Asadi

Abstract:

Detection and tracking of the lip contour is an important issue in speechreading. While there are solutions for lip tracking once a good contour initialization in the first frame is available, the problem of finding such a good initialization is not yet solved automatically, but done manually. We have developed a new tracking solution for lip contour detection using only few landmarks (15 to 25) and applying the well known Active Shape Models (ASM). The proposed method is a new LMS-like adaptive scheme based on an Auto regressive (AR) model that has been fit on the landmark variations in successive video frames. Moreover, we propose an extra motion compensation model to address more general cases in lip tracking. Computer simulations demonstrate a fair match between the true and the estimated spatial pixels. Significant improvements related to the well known LMS approach has been obtained via a defined Frobenius norm index.

Keywords: Lip contour, Tracking, LMS-Like

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557 An E-learning System Architecture based on Cloud Computing

Authors: Md. Anwar Hossain Masud, Xiaodi Huang

Abstract:

The massive proliferation of affordable computers, Internet broadband connectivity and rich education content has created a global phenomenon in which information and communication technology (ICT) is being used to transform education. Therefore, there is a need to redesign the educational system to meet the needs better. The advent of computers with sophisticated software has made it possible to solve many complex problems very fast and at a lower cost. This paper introduces the characteristics of the current E-Learning and then analyses the concept of cloud computing and describes the architecture of cloud computing platform by combining the features of E-Learning. The authors have tried to introduce cloud computing to e-learning, build an e-learning cloud, and make an active research and exploration for it from the following aspects: architecture, construction method and external interface with the model.

Keywords: Architecture, Cloud Computing, E-learning, Information Technology

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556 Green, Smooth and Easy Electrochemical Synthesis of N-Protected Indole Derivatives

Authors: Sarah Fahad Alajmi, Tamer Ezzat Youssef

Abstract:

Here, we report a simple method for the direct conversion of 6-Nitro-1H-indole into N-substituted indoles via electrochemical dehydrogenative reaction with halogenated reagents under strongly basic conditions through N–R bond formation. The N-protected indoles have been prepared under moderate and scalable electrolytic conditions. The conduct of the reactions was performed in a simple divided cell under constant current without oxidizing reagents or transition-metal catalysts. The synthesized products have been characterized via UV/Vis spectrophotometry, 1H-NMR, and FTIR spectroscopy. A possible reaction mechanism is discussed based on the N-protective products. This methodology could be applied to the synthesis of various biologically active N-substituted indole derivatives.

Keywords: Green chemistry, 1H-indole, NH-containing heteroaromatic, organic electrosynthesis.

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555 A 3.125Gb/s Clock and Data Recovery Circuit Using 1/4-Rate Technique

Authors: Il-Do Jeong, Hang-Geun Jeong

Abstract:

This paper describes the design and fabrication of a clock and data recovery circuit (CDR). We propose a new clock and data recovery which is based on a 1/4-rate frequency detector (QRFD). The proposed frequency detector helps reduce the VCO frequency and is thus advantageous for high speed application. The proposed frequency detector can achieve low jitter operation and extend the pull-in range without using the reference clock. The proposed CDR was implemented using a 1/4-rate bang-bang type phase detector (PD) and a ring voltage controlled oscillator (VCO). The CDR circuit has been fabricated in a standard 0.18 CMOS technology. It occupies an active area of 1 x 1 and consumes 90 mW from a single 1.8V supply.

Keywords: Clock and data recovery, 1/4-rate frequency detector, 1/4-rate phase detector.

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554 Retrieval Augmented Generation against the Machine: Merging Human Cyber Security Expertise with Generative AI

Authors: Brennan Lodge

Abstract:

Amidst a complex regulatory landscape, Retrieval Augmented Generation (RAG) emerges as a transformative tool for Governance Risk and Compliance (GRC) officers. This paper details the application of RAG in synthesizing Large Language Models (LLMs) with external knowledge bases, offering GRC professionals an advanced means to adapt to rapid changes in compliance requirements. While the development for standalone LLMs is exciting, such models do have their downsides. LLMs cannot easily expand or revise their memory, and they cannot straightforwardly provide insight into their predictions, and may produce “hallucinations.” Leveraging a pre-trained seq2seq transformer and a dense vector index of domain-specific data, this approach integrates real-time data retrieval into the generative process, enabling gap analysis and the dynamic generation of compliance and risk management content. We delve into the mechanics of RAG, focusing on its dual structure that pairs parametric knowledge contained within the transformer model with non-parametric data extracted from an updatable corpus. This hybrid model enhances decision-making through context-rich insights, drawing from the most current and relevant information, thereby enabling GRC officers to maintain a proactive compliance stance. Our methodology aligns with the latest advances in neural network fine-tuning, providing a granular, token-level application of retrieved information to inform and generate compliance narratives. By employing RAG, we exhibit a scalable solution that can adapt to novel regulatory challenges and cybersecurity threats, offering GRC officers a robust, predictive tool that augments their expertise. The granular application of RAG’s dual structure not only improves compliance and risk management protocols but also informs the development of compliance narratives with pinpoint accuracy. It underscores AI’s emerging role in strategic risk mitigation and proactive policy formation, positioning GRC officers to anticipate and navigate the complexities of regulatory evolution confidently.

Keywords: Retrieval Augmented Generation, Governance Risk and Compliance, Cybersecurity, AI-driven Compliance, Risk Management, Generative AI.

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553 An Effective Hybrid Genetic Algorithm for Job Shop Scheduling Problem

Authors: Bin Cai, Shilong Wang, Haibo Hu

Abstract:

The job shop scheduling problem (JSSP) is well known as one of the most difficult combinatorial optimization problems. This paper presents a hybrid genetic algorithm for the JSSP with the objective of minimizing makespan. The efficiency of the genetic algorithm is enhanced by integrating it with a local search method. The chromosome representation of the problem is based on operations. Schedules are constructed using a procedure that generates full active schedules. In each generation, a local search heuristic based on Nowicki and Smutnicki-s neighborhood is applied to improve the solutions. The approach is tested on a set of standard instances taken from the literature and compared with other approaches. The computation results validate the effectiveness of the proposed algorithm.

Keywords: Genetic algorithm, Job shop scheduling problem, Local search, Meta-heuristic algorithm

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552 Testing the Accuracy of ML-ANN for Harmonic Estimation in Balanced Industrial Distribution Power System

Authors: Wael M. El-Mamlouk, Metwally A. El-Sharkawy, Hossam. E. Mostafa

Abstract:

In this paper, we analyze and test a scheme for the estimation of electrical fundamental frequency signals from the harmonic load current and voltage signals. The scheme was based on using two different Multi Layer Artificial Neural Networks (ML-ANN) one for the current and the other for the voltage. This study also analyzes and tests the effect of choosing the optimum artificial neural networks- sizes which determine the quality and accuracy of the estimation of electrical fundamental frequency signals. The simulink tool box of the Matlab program for the simulation of the test system and the test of the neural networks has been used.

Keywords: Harmonics, Neural Networks, Modeling, Simulation, Active filters, electric Networks.

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551 Structural Analysis of an Active Morphing Wing for Enhancing UAV Performance

Authors: E. Kaygan, A. Gatto

Abstract:

A numerical study of a design concept for actively controlling wing twist is described in this paper. The concept consists of morphing elements which were designed to provide a rigid and seamless skin while maintaining structural rigidity. The wing structure is first modeled in CATIA V5 then imported into ANSYS for structural analysis. Athena Vortex Lattice method (AVL) is used to estimate aerodynamic response as well as aerodynamic loads of morphing wings, afterwards a structural optimization performed via ANSYS Static. Overall, the results presented in this paper show that the concept provides efficient wing twist while preserving an aerodynamically smooth and compliant surface. Sufficient structural rigidity in bending is also obtained. This concept is suggested as a possible alternative for morphing skin applications. 

Keywords: Aircraft, morphing, skin, twist.

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550 Electron Filling Factor and Sunlight Concentration Effects on the Efficiency of Intermediate Band Solar Cell

Authors: Nima Es'haghi Gorji, Hossein Movla, Foozieh Sohrabi, Alireza Mottaghizadeh, Mohammad Houshmand, Hassan Babaei, Arash Nikniazi

Abstract:

For a determined intermediate band position, the effects of electron filling factor and sunlight concentration on the active region thickness and efficiency of the quantum-dot intermediate band solar cell are calculated. For each value of electron filling factor, the maximum point of efficiency obtained and resulted in the optimum thickness of the cell under three different sunlight concentrations. We show the importance of filling factor as a parameter to be more considered. The photon recycling effect eliminated in all calculations.

Keywords: Intermediate band, Sunlight concentration, Efficiency limits, Electron filling factor

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549 Impact of Normative Institutional Factors on Sustainability Reporting

Authors: L. Dagilienė

Abstract:

The article explores the impact of normative institutional factors on the development of sustainability reporting. The vast majority of research in the scientific literature focuses on mandatory institutional factors, i.e. how public institutions and market regulators affect sustainability reporting. Meanwhile, there is lack of empirical data for the impact of normative institutional factors. The effect of normative factors in this paper is based on the role of non-governmental organizations (NGO) and institutional theory. The case of Global Compact Local Network in the developing country was examined. The research results revealed that in the absence of regulated factors, companies were not active with regard to social disclosures; they presented non-systemized social information of a descriptive nature. Only 10% of sustainability reports were prepared using the GRI methodology. None of the reports were assured by third parties.

Keywords: Institutional theory, normative, sustainability reporting.

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548 A Quantitative Study of the Evolution of Open Source Software Communities

Authors: M. R. Martinez-Torres, S. L. Toral, M. Olmedilla

Abstract:

Typically, virtual communities exhibit the well-known phenomenon of participation inequality, which means that only a small percentage of users is responsible of the majority of contributions. However, the sustainability of the community requires that the group of active users must be continuously nurtured with new users that gain expertise through a participation process. This paper analyzes the time evolution of Open Source Software (OSS) communities, considering users that join/abandon the community over time and several topological properties of the network when modeled as a social network. More specifically, the paper analyzes the role of those users rejoining the community and their influence in the global characteristics of the network.

Keywords: Open source communities, social network analysis, time series, virtual communities.

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547 Recycled Waste Glass Powder as a Partial Cement Replacement in Polymer-Modified Mortars

Authors: Nikol Žižková

Abstract:

The aim of this study was to observe the behavior of polymer-modified cement mortars with regard to the use of a pozzolanic admixture. Polymer-modified mortars (PMMs) containing various types of waste glass (waste packing glass and fluorescent tube glass) were produced always with 20% of cement substituted with a pozzolanic-active material. Ethylene/vinyl acetate copolymer (EVA) was used for polymeric modification. The findings confirm the possibility of using the waste glass examined herein as a partial substitute for cement in the production of PMM, which contributes to the preservation of non-renewable raw material resources and to the efficiency of waste glass material reuse.

Keywords: Recycled waste glass, polymer-modified mortars, pozzolanic admixture.

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546 Increasing Lifetime of Target Tracking Wireless Sensor Networks

Authors: Khin Thanda Soe

Abstract:

A model to identify the lifetime of target tracking wireless sensor network is proposed. The model is a static clusterbased architecture and aims to provide two factors. First, it is to increase the lifetime of target tracking wireless sensor network. Secondly, it is to enable good localization result with low energy consumption for each sensor in the network. The model consists of heterogeneous sensors and each sensing member node in a cluster uses two operation modes–active mode and sleep mode. The performance results illustrate that the proposed architecture consumes less energy and increases lifetime than centralized and dynamic clustering architectures, for target tracking sensor network.

Keywords: Network lifetime, Target Localization, TargetTracking, Wireless Sensor Networks.

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545 A New Current-mode Multifunction Filter with High Impedance Outputs Using Minimum Number of Passive Elements

Authors: Mehmet Sagbas, Kemal Fidanboylu, Mehmet C. Bayram

Abstract:

A new current-mode multifunction filter using minimum number of passive elements is proposed. The proposed filter has single-input and four high-impedance outputs. It uses four passive elements (two capacitors and two resistors) and four dual output second generation current conveyors. Each output provides a different filter response, namely, low-pass, high-pass, band-pass and band-reject. The sensitivity analysis is also carried out on both ideal and non-ideal filter configurations. The validity of the proposed filter is verified through PSPICE simulations.

Keywords: Active filter, Universal filter, Currentconveyors.

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544 Gesture Recognition by Data Fusion of Time-of-Flight and Color Cameras

Authors: Piercarlo Dondi, Luca Lombardi, Marco Porta

Abstract:

In the last years numerous applications of Human- Computer Interaction have exploited the capabilities of Time-of- Flight cameras for achieving more and more comfortable and precise interactions. In particular, gesture recognition is one of the most active fields. This work presents a new method for interacting with a virtual object in a 3D space. Our approach is based on the fusion of depth data, supplied by a ToF camera, with color information, supplied by a HD webcam. The hand detection procedure does not require any learning phase and is able to concurrently manage gestures of two hands. The system is robust to the presence in the scene of other objects or people, thanks to the use of the Kalman filter for maintaining the tracking of the hands.

Keywords: Gesture recognition, human-computer interaction, Time-of-Flight camera.

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543 Numerical Study of Flow Separation Control over a NACA2415 Airfoil

Authors: M. Tahar Bouzaher

Abstract:

This study involves numerical simulation of the flow around a NACA2415 airfoil, with a 18° angle of attack, and flow separation control using a rod, It involves putting a cylindrical rod - upstream of the leading edge- in vertical translation movement in order to accelerate the transition of the boundary layer by interaction between the rod wake and the boundary layer. The viscous, nonstationary flow is simulated using ANSYS FLUENT 13. The rod movement is reproduced using the dynamic mesh technique and an in-house developed UDF (User Define Function). The frequency varies from 75 to 450 Hz and the considered amplitudes are 2%, and 3% of the foil chord. The frequency chosen closed to the frequency of separation. Our results showed a substantial modification in the flow behavior and a maximum drag reduction of 61%.

Keywords: CFD, Flow separation, Active control, Boundary layer, rod, NACA 2415.

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542 Organic Contribution on Particles Formed on Pacific Ocean: From Phytoplankton Blooms to Climate

Authors: Petri Vaattovaara, Luke Cravigan, Zoran Ristovski, Marc Mallet, Ari Laaksonen, Sarah Lawson, Nick Talbot, Gustavo Olivares, Mike Harvey, Cliff Law

Abstract:

These SOAP project Pacific Ocean measurements reveal that phytoplankton blooms with sunny conditions make possible secondary organic contribution to ultrafine particles size and composition, and thus on cloud formation ability, and finally on climate. This is in agreement with other biologically active region observations about the presence of secondary organics even the exact fraction is also depending on the local marine life (e.g. plankton blooms, seaweeds, corals). An organic contribution is clearly needed to add to CLAW hypothesis.

Keywords: Climate, marine aerosols, phytoplankton, secondary organics, CLAW hypothesis.

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541 The Anti-Noise and Anti-Wear Systems for Railways

Authors: Brigita Altenbaher

Abstract:

In recent years there has been a continuous increase of axle loads, tonnage, train speed and train length which has increased both the productivity in the rail sector and the risk of rail breaks and derailments. On the other hand, the environmental requirements (e.g. noise reduction) for railway operations will become tighter in the future. In our research we developed a new composite material which does not change braking properties, is capable of taking extremely high pressure loads, reduces noise and is environmentally friendly. Part of our research was also the development of technology which will be able to apply this material to the rail. The result of our research was the system which reduces the wear out significantly and almost completely eliminates the squealing noise at the same time, and by using only one special material.

Keywords: Active protection, composite material, lubrication, noise reduction, reduction at source, railway.

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540 MAP-Based Image Super-resolution Reconstruction

Authors: Xueting Liu, Daojin Song, Chuandai Dong, Hongkui Li

Abstract:

From a set of shifted, blurred, and decimated image , super-resolution image reconstruction can get a high-resolution image. So it has become an active research branch in the field of image restoration. In general, super-resolution image restoration is an ill-posed problem. Prior knowledge about the image can be combined to make the problem well-posed, which contributes to some regularization methods. In the regularization methods at present, however, regularization parameter was selected by experience in some cases and other techniques have too heavy computation cost for computing the parameter. In this paper, we construct a new super-resolution algorithm by transforming the solving of the System stem Є=An into the solving of the equations X+A*X-1A=I , and propose an inverse iterative method.

Keywords: High-resolution MAP image, Reconstruction, Image interpolation, Motion Estimation, Hermitian positive definite solutions.

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539 Predictability Analysis on HIV/AIDS System using Hurst Exponents

Authors: K. Kamalanand, P. Mannar Jawahar

Abstract:

Methods of contemporary mathematical physics such as chaos theory are useful for analyzing and understanding the behavior of complex biological and physiological systems. The three dimensional model of HIV/AIDS is the basis of active research since it provides a complete characterization of disease dynamics and the interaction of HIV-1 with the immune system. In this work, the behavior of the HIV system is analyzed using the three dimensional HIV model and a chaotic measure known as the Hurst exponent. Results demonstrate that Hurst exponents of CD4, CD8 cells and viral load vary nonlinearly with respect to variations in system parameters. Further, it was observed that the three dimensional HIV model can accommodate both persistent (H>0.5) and anti-persistent (H<0.5) dynamics of HIV states. In this paper, the objectives of the study, methodology and significant observations are presented in detail.

Keywords: HIV/AIDS, mathematical model, chaos theory, Hurst exponent

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538 Mathematical Modeling of Cell Volume Alterations under Different Osmotic Conditions

Authors: Juliana A. Knocikova, Yann Bouret, Médéric Argentina, Laurent Counillon

Abstract:

Cell volume, together with membrane potential and intracellular hydrogen ion concentration, is an essential biophysical parameter for normal cellular activity. Cell volumes can be altered by osmotically active compounds and extracellular tonicity. In this study, a simple mathematical model of osmotically induced cell swelling and shrinking is presented. Emphasis is given to water diffusion across the membrane. The mathematical description of the cellular behavior consists in a system of coupled ordinary differential equations. We compare experimental data of cell volume alterations driven by differences in osmotic pressure with mathematical simulations under hypotonic and hypertonic conditions. Implications for a future model are also discussed.

Keywords: Eukaryotic cell, mathematical modeling, osmosis, volume alterations.

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537 CFD Analysis of Passive Cooling Building by Using Solar Chimney System

Authors: Naci Kalkan, Ihsan Dagtekin

Abstract:

This research presents the design and analysis of solar air-conditioning systems particularly solar chimney which is a passive strategy for natural ventilation, and demonstrates the structures of these systems’ using Computational Fluid Dynamic (CFD) and finally compares the results with several examples, which have been studied experimentally and carried out previously. In order to improve the performance of solar chimney system, highly efficient sub-system components are considered for the design. The general purpose of the research is to understand how efficiently solar chimney systems generate cooling, and is to improve the efficient of such systems for integration with existing and future domestic buildings.

Keywords: Solar cooling system, solar chimney, active and passive solar technologies, natural ventilation, cavity depth, CFD models for solar chimney.

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536 Virtual Gesture Screen System Based on 3D Visual Information and Multi-Layer Perceptron

Authors: Yang-Keun Ahn, Min-Wook Kim, Young-Choong Park, Kwang-Soon Choi, Woo-Chool Park, Hae-Moon Seo, Kwang-Mo Jung

Abstract:

Active research is underway on virtual touch screens that complement the physical limitations of conventional touch screens. This paper discusses a virtual touch screen that uses a multi-layer perceptron to recognize and control three-dimensional (3D) depth information from a time of flight (TOF) camera. This system extracts an object-s area from the image input and compares it with the trajectory of the object, which is learned in advance, to recognize gestures. The system enables the maneuvering of content in virtual space by utilizing human actions.

Keywords: Gesture Recognition, Depth Sensor, Virtual Touch Screen

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535 CSR of top Portuguese Companies: Relation between Social Performance and Economic Performance

Authors: Afonso, S. C., Fernandes, P. O., Monte, A. P.

Abstract:

Modern times call organizations to have an active role in the social arena, through Corporate Social Responsibility (CSR). The objective of this research was to test the hypothesis that there is a positive relation between social performance and economic performance, and if there is a positive correlation between social performance and financial-economic performance. To test these theories a measure of social performance, based on the Green Book of Commission of the European Community, was used in a group of nineteen Portuguese top companies, listed on the PSI 20 index, through a period of five years, since 2005 to 2009. A clusters analysis was applied to group companies by their social performance and to compare and correlate their economic performance. Results indicate that companies that had a better social performance are not the ones who had a better economic performance, and suggest that the middle path might provide a good relation CSR-Economic performance, as a basis to a sustainable development.

Keywords: Corporate Social Responsibility, Economic Performance, Win-Win relationship

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534 Meta Random Forests

Authors: Praveen Boinee, Alessandro De Angelis, Gian Luca Foresti

Abstract:

Leo Breimans Random Forests (RF) is a recent development in tree based classifiers and quickly proven to be one of the most important algorithms in the machine learning literature. It has shown robust and improved results of classifications on standard data sets. Ensemble learning algorithms such as AdaBoost and Bagging have been in active research and shown improvements in classification results for several benchmarking data sets with mainly decision trees as their base classifiers. In this paper we experiment to apply these Meta learning techniques to the random forests. We experiment the working of the ensembles of random forests on the standard data sets available in UCI data sets. We compare the original random forest algorithm with their ensemble counterparts and discuss the results.

Keywords: Random Forests [RF], ensembles, UCI.

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533 An Implicit Region-Based Deformable Model with Local Segmentation Applied to Weld Defects Extraction

Authors: Y. Boutiche, N. Ramou, M. Ben Gharsallah

Abstract:

This paper is devoted to present and discuss a model that allows a local segmentation by using statistical information of a given image. It is based on Chan-Vese model, curve evolution, partial differential equations and binary level sets method. The proposed model uses the piecewise constant approximation of Chan-Vese model to compute Signed Pressure Force (SPF) function, this one attracts the curve to the true object(s)-s boundaries. The implemented model is used to extract weld defects from weld radiographic images in the aim to calculate the perimeter and surfaces of those weld defects; encouraged resultants are obtained on synthetic and real radiographic images.

Keywords: Active contour, Chan-Vese Model, local segmentation, weld radiographic images.

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532 Fractional Order Controller Design for Vibration Attenuation in an Airplane Wing

Authors: Birs Isabela, Muresan Cristina, Folea Silviu, Prodan Ovidiu

Abstract:

The wing is one of the most important parts of an airplane because it ensures stability, sustenance and maneuverability of the airplane. Because of its shape, the airplane wing can be simplified to a smart beam. Active vibration suppression is realized using piezoelectric actuators that are mounted on the surface of the beam. This work presents a tuning procedure of fractional order controllers based on a graphical approach of the frequency domain representation. The efficacy of the method is proven by practically testing the controller on a laboratory scale experimental stand.

Keywords: Fractional order controller, piezoelectric actuators, smart beam, vibration suppression.

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531 Family Structure between Muslim and Santal Communities in Rural Bangladesh

Authors: Md. Emaj Uddin

Abstract:

Family structure that is culturally constructed in every society is the basic unit of social structure. Purpose of the study was to compare family structure, including marriage, residence, family size, type, role sharing, authority, and communication patterns between Muslim and Santal communities in rural Bangladesh. For this we assumed that family structure with the elements was significantly different between the two communities in rural Bangladesh. In so doing, 288 active couples (145 for Muslim and 143 for Santal) selected by cluster random sampling were intensively interviewed with a semi-structured questionnaire method. The results of Pearson Chi-Squire Test reveal that there were significant differences in the family structure followed by the two communities in the study area. Further cross-cultural study should be done on why family structure varies between the communities in Bangladesh.

Keywords: Bangladesh, Cross-Cultural Comparison, Family Structure, Muslim, Santal.

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530 Inhibition on Conidial Germination of Colletotrichum gloeosporiodes and Pestalotiopsis eugeniae by Bacillus subtilis LB5

Authors: Onuma Ruangwong, Wen-Jinn Liang, S.Y. Zhang, Chi-I Chang

Abstract:

The effect of antifungal compound from Bacillus subtilis strain LB5 was tested against conidial germination of Colletotrichum gloeosporioides and Pestalotiopsis eugeniae, causal agent of anthracnose and fruit rot of wax apple, respectively. Observation under scanning electron microscope and light compound microscope revealed that conidial germination was completely inhibited when treated with culture broth, culture filtrate, or crude extract from strain LB5. Identification of purified antifungal compound produced by strain LB5 in cell-free supernatant by nuclear magnetic resonance and fast atom bombardment showed that the active compound was iturin A-2.

Keywords: Iturin A-2, Bacillus subtilis LB5, Colleteotrichum gloeospporioides, Pestalotiopsis eugeniae, wax apple

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