Search results for: Zheng Wang
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 731

Search results for: Zheng Wang

731 Investigation of the Aerodynamic Characters of Ducted Fan System

Authors: Wang Bo , Guo Zheng , Wang Peng , Shan Shangqiu , Hou Zhongxi

Abstract:

This paper investigates the aerodynamic characters of a model ducted fan system, analyses the basic principle of the effect of thrust promotion and torque reduction, discovers the relationship between the revolutions per minute(RPM) of the fan and the characters of thrust, as well as system torque. Firstly a model ducted fan has been designed and manufactured according to the specific structure of flow field, then CFD simulation has been carried out to analyze such aerodynamics, finally bench tests have been used to validate the simulation results and system configuration.

Keywords: ducted fan, free vortex flow, stator blade, screw torque, thrust increase

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730 Bluetooth Piconet System for Child Care Applications

Authors: Ching-Sung Wang, Teng-Wei Wang, Zhen-Ting Zheng

Abstract:

This study mainly concerns a safety device designed for child care. When children are out of sight or the caregivers cannot always pay attention to the situation, through the functions of this device, caregivers can immediately be informed to make sure that the children do not get lost or hurt, and thus, ensure their safety. Starting from this concept, a device is produced based on the relatively low-cost Bluetooth piconet system and a three-axis gyroscope sensor. This device can transmit data to a mobile phone app through Bluetooth, in order that the user can learn the situation at any time. By simply clipping the device in a pocket or on the waist, after switching on/starting the device, it will send data to the phone to detect the child’s fall and distance. Once the child is beyond the angle or distance set by the app, it will issue a warning to inform the phone owner.

Keywords: Children care, piconet system, three-axis gyroscope, distance detection, falls detection.

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729 Research and Development of Net-Centric Information Sharing Platform

Authors: Xiaoqing Wang, Fang Youyuan, Zheng Yanxing, Gu Tianyang, Zong Jianjian, Tong Jinrong

Abstract:

Compared with traditional distributed environment, the net-centric environment brings on more demanding challenges for information sharing with the characteristics of ultra-large scale and strong distribution, dynamic, autonomy, heterogeneity, redundancy. This paper realizes an information sharing model and a series of core services, through which provides an open, flexible and scalable information sharing platform.

Keywords: Net-centric environment, Information sharing, Metadata registry and catalog, Cross-domain data access control.

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728 An Iterative Algorithm for KLDA Classifier

Authors: D.N. Zheng, J.X. Wang, Y.N. Zhao, Z.H. Yang

Abstract:

The Linear discriminant analysis (LDA) can be generalized into a nonlinear form - kernel LDA (KLDA) expediently by using the kernel functions. But KLDA is often referred to a general eigenvalue problem in singular case. To avoid this complication, this paper proposes an iterative algorithm for the two-class KLDA. The proposed KLDA is used as a nonlinear discriminant classifier, and the experiments show that it has a comparable performance with SVM.

Keywords: Linear discriminant analysis (LDA), kernel LDA (KLDA), conjugate gradient algorithm, nonlinear discriminant classifier.

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727 Recommended Practice for Experimental Evaluation of the Seepage Sensitivity Damage of Coalbed Methane Reservoirs

Authors: Hao Liu, Lihui Zheng, Chinedu J. Okere, Chao Wang, Xiangchun Wang, Peng Zhang

Abstract:

The coalbed methane (CBM) extraction industry (an unconventional energy source) has not established guidelines for experimental evaluation of sensitivity damage for coal samples. The existing experimental process of previous researches mainly followed the industry standard for conventional oil and gas reservoirs (CIS). However, the existing evaluation method ignores certain critical differences between CBM reservoirs and conventional reservoirs, which could inevitably result in an inaccurate evaluation of sensitivity damage and, eventually, poor decisions regarding the formulation of formation damage prevention measures. In this study, we propose improved experimental guidelines for evaluating seepage sensitivity damage of CBM reservoirs by leveraging on the shortcomings of the existing methods. The proposed method was established via a theoretical analysis of the main drawbacks of the existing methods and validated through comparative experiments. The results show that the proposed evaluation technique provided reliable experimental results that can better reflect actual reservoir conditions and correctly guide the future development of CBM reservoirs. This study is pioneering the research on the optimization of experimental parameters for efficient exploration and development of CBM reservoirs.

Keywords: Coalbed methane, formation damage, permeability, unconventional energy source.

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726 A Functional Framework for Large Scale Application Software Systems

Authors: Han-hua Lu, Shun-yi Zhang, Yong Zheng, Ya-shi Wang, Li-juan Min

Abstract:

From the perspective of system of systems (SoS) and emergent behaviors, this paper describes large scale application software systems, and proposes framework methods to further depict systems- functional and non-functional characteristics. Besides, this paper also specifically discusses some functional frameworks. In the end, the framework-s applications in system disintegrations, system architecture and stable intermediate forms are additionally dealt with in this in building, deployment and maintenance of large scale software applications.

Keywords: application software system, framework methods, system of systems, emergent behaviors

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725 Low Frequency Noise Behavior of Independent Gate Junctionless FinFET

Authors: A. Kamath, Z. X. Chen, C. J. Gu, F. Zheng, X. P. Wang, N. Singh, G-Q. Lo

Abstract:

In this paper we use low frequency noise analysis to understand and map the current conduction path in a multi gate junctionless FinFET.  The device used in this study behaves as a gated resistor and shows excellent short channel effect suppression due to its multi gate structure. Generally for a bulk conduction device like the junctionless device studied in this work, the low frequency noise can be modelled using the mobility fluctuation model; however for this device we can also see the effect of carrier fluctuations on the LFN characteristic. The noise characteristic at different gate bias and also the possible location of the traps is explained.

Keywords: LFN analysis, junctionless, Current conduction path, FinFET.

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724 Integration of Resistive Switching Memory Cell with Vertical Nanowire Transistor

Authors: Xiang Li, Zhixian Chen, Zheng Fang, Aashit Kamath, Xinpeng Wang, Navab Singh, Guo-Qiang Lo, Dim-Lee Kwong

Abstract:

We integrate TiN/Ni/HfO2/Si RRAM cell with a vertical gate-all-around (GAA) nanowire transistor to achieve compact 4F2 footprint in a 1T1R configuration. The tip of the Si nanowire (source of the transistor) serves as bottom electrode of the memory cell. Fabricated devices with nanowire diameter ~ 50nm demonstrate ultra-low current/power switching; unipolar switching with 10μA/30μW SET and 20μA/30μW RESET and bipolar switching with 20nA/85nW SET and 0.2nA/0.7nW RESET. Further, the switching current is found to scale with nanowire diameter making the architecture promising for future scaling.

Keywords: RRAM, 1T1R, gate-all-around FET, nanowire FET, vertical MOSFETs

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723 Urban Management and China's Municipal Pattern

Authors: Ling Zheng, Yaping Wei, Kang Cao, Zheng Huang, Songpo Shi

Abstract:

Not only is municipal pattern the institution basement of urban management, but it also determines the forms of the management results. There-s a considerable possibility of bankruptcy for China-s current municipal pattern as it-s an overdraft of land deal in fact. Based on the analysis of China-s current municipal pattern, the passage proposed an assumption of a new pattern verified legitimacy by conceptual as well as econometric models. Conclusion is: the added supernumerary value of investment in public goods was not included in China-s current municipal pattern, but hidden in the rising housing prices; we should set housing tax or municipal tax to optimize the municipal pattern, to correct the behavior of local governments and to ensure the regular development of China-s urbanization.

Keywords: Urban management, China's municipal pattern, land financial institution, housing tax, Public goods.

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722 Optimum Design of Launching Nose during Incremental Launching Construction of Same-Span Continuous Bridge

Authors: Weifeng Wang, Hengbin Zheng, Xianwei Zeng

Abstract:

The launching nose plays an important role in the incremental launching construction. The parameters of the launching nose essentially affect the internal forces of the girder during the construction. The appropriate parameters can decrease the internal forces in the girder and save the material and reduce the cost. The simplified structural model, which is made with displacement method according to the characteristic of incremental launching construction and the variation rule of the internal forces, calculates and analyzes the effect of the length, the rigidity and weight of launch nose on the internal forces of girder during the incremental launching construction. The method, which can calculate the launching nose parameters for the optimum incremental launching construction, is achieved. This method is simple, reliable and easy for practical use.

Keywords: incremental launching, launching nose, optimumanalysis, displacement method

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721 Frequency-Dependent and Full Range Tunable Phase Shifter

Authors: Yufu Yin, Tao Lin, Shanghong Zhao, Zihang Zhu, Xuan Li, Wei Jiang, Qiurong Zheng, Hui Wang

Abstract:

In this paper, a frequency-dependent and tunable phase shifter is proposed and numerically analyzed. The key devices are the dual-polarization binary phase shift keying modulator (DP-BPSK) and the fiber Bragg grating (FBG). The phase-frequency response of the FBG is employed to determine the frequency-dependent phase shift. The simulation results show that a linear phase shift of the recovered output microwave signal which depends on the frequency of the input RF signal is achieved. In addition, by adjusting the power of the RF signal, the full range phase shift from 0° to 360° can be realized. This structure shows the spurious free dynamic range (SFDR) of 70.90 dB·Hz2/3 and 72.11 dB·Hz2/3 under different RF powers.

Keywords: Microwave photonics, phase shifter, spurious free dynamic range, frequency-dependent.

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720 Fast and Efficient Algorithms for Evaluating Uniform and Nonuniform Lagrange and Newton Curves

Authors: Taweechai Nuntawisuttiwong, Natasha Dejdumrong

Abstract:

Newton-Lagrange Interpolations are widely used in numerical analysis. However, it requires a quadratic computational time for their constructions. In computer aided geometric design (CAGD), there are some polynomial curves: Wang-Ball, DP and Dejdumrong curves, which have linear time complexity algorithms. Thus, the computational time for Newton-Lagrange Interpolations can be reduced by applying the algorithms of Wang-Ball, DP and Dejdumrong curves. In order to use Wang-Ball, DP and Dejdumrong algorithms, first, it is necessary to convert Newton-Lagrange polynomials into Wang-Ball, DP or Dejdumrong polynomials. In this work, the algorithms for converting from both uniform and non-uniform Newton-Lagrange polynomials into Wang-Ball, DP and Dejdumrong polynomials are investigated. Thus, the computational time for representing Newton-Lagrange polynomials can be reduced into linear complexity. In addition, the other utilizations of using CAGD curves to modify the Newton-Lagrange curves can be taken.

Keywords: Newton interpolation, Lagrange interpolation, linear complexity.

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719 Urban-Rural Balance, Regional Coordination and Land Transfer in China

Authors: Ling Zheng, Yaping Wei, Kang Cao, Songpo Shi, Jinxing Wang

Abstract:

It-s difficult for China-s current land transfer institutions limited to county-wide to solve the contradiction between urban-rural development and construction land shortage. On the basis of analyzing China-s construction land transfer system, and evaluation toward Transfer of development rights (TDR) practices in Anhui and Chongqing, the passage proposes: (1) we should establish a multi-level land indicators trade market under the guidance of regional spatial objectives, and allow land transfer paid across cities and counties within a specific area following the regulation of both government and market; (2) it would be better to combine organically the policy ntentions of land plan, regional plan, urban plan and economic plan, and link them with land indicators transfer to promote a wider range of urban-rural balance and regional coordination.

Keywords: China's land institutions, transfer of development rights, urban-rural balance, regional coordination.

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718 Determinants of Consumer Choice for Online Travel Shopping Sites: A Confirmatory Analysis

Authors: Yu-Min Wang

Abstract:

This study attempts to validate the consumer-oriented criteria list, developed by Wang et al. (2010), for selecting online travel shopping sites. Based on a sample of 985 respondents, confirmatory factor analysis was employed to test the factor structure and assess the reliability and validity of the list. The results support the list developed by Wang et al. (2010) and claim the list can be further used to analyze, explain, and understand consumer behaviors about online travel shopping.

Keywords: Online travel agency, online travel shopping sites, confirmatory analysis.

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717 An Internet of Things-Based Weight Monitoring System for Honey

Authors: Zheng-Yan Ruan, Chien-Hao Wang, Hong-Jen Lin, Chien-Peng Huang, Ying-Hao Chen, En-Cheng Yang, Chwan-Lu Tseng, Joe-Air Jiang

Abstract:

Bees play a vital role in pollination. This paper focuses on the weighing process of honey. Honey is usually stored at the comb in a hive. Bee farmers brush bees away from the comb and then collect honey, and the collected honey is weighed afterward. However, such a process brings strong negative influences on bees and even leads to the death of bees. This paper therefore presents an Internet of Things-based weight monitoring system which uses weight sensors to measure the weight of honey and simplifies the whole weighing procedure. To verify the system, the weight measured by the system is compared to the weight of standard weights used for calibration by employing a linear regression model. The R2 of the regression model is 0.9788, which suggests that the weighing system is highly reliable and is able to be applied to obtain actual weight of honey. In the future, the weight data of honey can be used to find the relationship between honey production and different ecological parameters, such as bees’ foraging behavior and weather conditions. It is expected that the findings can serve as critical information for honey production improvement.

Keywords: Internet of Things, weight, honey, bee.

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716 An Integrative Bayesian Approach to Supporting the Prediction of Protein-Protein Interactions: A Case Study in Human Heart Failure

Authors: Fiona Browne, Huiru Zheng, Haiying Wang, Francisco Azuaje

Abstract:

Recent years have seen a growing trend towards the integration of multiple information sources to support large-scale prediction of protein-protein interaction (PPI) networks in model organisms. Despite advances in computational approaches, the combination of multiple “omic" datasets representing the same type of data, e.g. different gene expression datasets, has not been rigorously studied. Furthermore, there is a need to further investigate the inference capability of powerful approaches, such as fullyconnected Bayesian networks, in the context of the prediction of PPI networks. This paper addresses these limitations by proposing a Bayesian approach to integrate multiple datasets, some of which encode the same type of “omic" data to support the identification of PPI networks. The case study reported involved the combination of three gene expression datasets relevant to human heart failure (HF). In comparison with two traditional methods, Naive Bayesian and maximum likelihood ratio approaches, the proposed technique can accurately identify known PPI and can be applied to infer potentially novel interactions.

Keywords: Bayesian network, Classification, Data integration, Protein interaction networks.

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715 Creeping Control Strategy for Direct Shift Gearbox Based on the Investigation of Temperature Variation of the Wet Clutch

Authors: Biao Ma, Jikai Liu, Man Chen, Jianpeng Wu, Liyong Wang, Changsong Zheng

Abstract:

Proposing an appropriate control strategy is an effective and practical way to address the overheat problems of the wet multi-plate clutch in Direct Shift Gearbox under the long-time creeping condition. To do so, the temperature variation of the wet multi-plate clutch is investigated firstly by establishing a thermal resistance model for the gearbox cooling system. To calculate the generated heat flux and predict the clutch temperature precisely, the friction torque model is optimized by introducing an improved friction coefficient, which is related to the pressure, the relative speed and the temperature. After that, the heat transfer model and the reasonable friction torque model are employed by the vehicle powertrain model to construct a comprehensive co-simulation model for the Direct Shift Gearbox (DSG) vehicle. A creeping control strategy is then proposed and, to evaluate the vehicle performance, the safety temperature (250 ℃) is particularly adopted as an important metric. During the creeping process, the temperature of two clutches is always under the safety value (250 ℃), which demonstrates the effectiveness of the proposed control strategy in avoiding the thermal failures of clutches.

Keywords: Creeping control strategy, direct shift gearbox, temperature variation, wet clutch.

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714 The Lower and Upper Approximations in a Group

Authors: Zhaohao Wang, Lan Shu

Abstract:

In this paper, we generalize some propositions in [C.Z. Wang, D.G. Chen, A short note on some properties of rough groups, Comput. Math. Appl. 59(2010)431-436.] and we give some equivalent conditions for rough subgroups. The notion of minimal upper rough subgroups is introduced and a equivalent characterization is given, which implies the rough version of Lagranges Theorem.

Keywords: Lower approximations, Upper approximations, Rough sets, Rough groups, Lagrange

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713 Towards Growing Self-Organizing Neural Networks with Fixed Dimensionality

Authors: Guojian Cheng, Tianshi Liu, Jiaxin Han, Zheng Wang

Abstract:

The competitive learning is an adaptive process in which the neurons in a neural network gradually become sensitive to different input pattern clusters. The basic idea behind the Kohonen-s Self-Organizing Feature Maps (SOFM) is competitive learning. SOFM can generate mappings from high-dimensional signal spaces to lower dimensional topological structures. The main features of this kind of mappings are topology preserving, feature mappings and probability distribution approximation of input patterns. To overcome some limitations of SOFM, e.g., a fixed number of neural units and a topology of fixed dimensionality, Growing Self-Organizing Neural Network (GSONN) can be used. GSONN can change its topological structure during learning. It grows by learning and shrinks by forgetting. To speed up the training and convergence, a new variant of GSONN, twin growing cell structures (TGCS) is presented here. This paper first gives an introduction to competitive learning, SOFM and its variants. Then, we discuss some GSONN with fixed dimensionality, which include growing cell structures, its variants and the author-s model: TGCS. It is ended with some testing results comparison and conclusions.

Keywords: Artificial neural networks, Competitive learning, Growing cell structures, Self-organizing feature maps.

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712 Comparing Hilditch, Rosenfeld, Zhang-Suen,and Nagendraprasad -Wang-Gupta Thinning

Authors: Anastasia Rita Widiarti

Abstract:

This paper compares Hilditch, Rosenfeld, Zhang- Suen, dan Nagendraprasad Wang Gupta (NWG) thinning algorithms for Javanese character image recognition. Thinning is an effective process when the focus in not on the size of the pattern, but rather on the relative position of the strokes in the pattern. The research analyzes the thinning of 60 Javanese characters. Time-wise, Zhang-Suen algorithm gives the best results with the average process time being 0.00455188 seconds. But if we look at the percentage of pixels that meet one-pixel thickness, Rosenfelt algorithm gives the best results, with a 99.98% success rate. From the number of pixels that are erased, NWG algorithm gives the best results with the average number of pixels erased being 84.12%. It can be concluded that the Hilditch algorithm performs least successfully compared to the other three algorithms.

Keywords: Hilditch algorithm, Nagendraprasad-Wang-Guptaalgorithm, Rosenfeld algorithm, Thinning, Zhang-suen algorithm

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711 On a New Nonlinear Sum-difference Inequality with Application

Authors: Kelong Zheng, Shouming Zhong

Abstract:

A new nonlinear sum-difference inequality in two variables which generalize some existing results and can be used as handy tools in the analysis of certain partial difference equation is discussed. An example to show boundedness of solutions of a difference value problem is also given.

Keywords: Sum-Difference inequality, Nonlinear, Boundedness.

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710 Preconditioned Jacobi Method for Fuzzy Linear Systems

Authors: Lina Yan, Shiheng Wang, Ke Wang

Abstract:

A preconditioned Jacobi (PJ) method is provided for solving fuzzy linear systems whose coefficient matrices are crisp Mmatrices and the right-hand side columns are arbitrary fuzzy number vectors. The iterative algorithm is given for the preconditioned Jacobi method. The convergence is analyzed with convergence theorems. Numerical examples are given to illustrate the procedure and show the effectiveness and efficiency of the method.

Keywords: preconditioning, M-matrix, Jacobi method, fuzzy linear system (FLS).

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709 A Study on the Relation of Corporate Governance and Pricing for Initial Public Offerings

Authors: Chei-Chang Chiou, Sen-Wei Wang, Yu-Min Wang

Abstract:

The purpose of this study is to investigate the relationship between corporate governance and pricing for initial public offerings (IPOs). Empirical result finds that the prediction of pricing of IPOs with corporate governance added can have a rather higher degree of predicting accuracy than that of non governance added during the training and testing samples. Therefore, it can be observed that corporate governance mechanism can affect the pricing of IPOs

Keywords: Artificial neural networks, corporate governance, initial public offerings.

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708 Sulfamonomethoxine-Induced Urinary Calculiin Pigs

Authors: Wei-Dong Sun, Ke-Chun Zhang, Jin-Yong Wang, Xiao-Long Wang

Abstract:

The authors report a case of swine urolithiasis caused by improper administration of sulfamonomethoxine and which was diagnosed by examination of urinary sediments and analyzing the composition of the uroliths. The chemical composition of urinary calculi obtained from affected pigs with urolithiasis was further confimed as sulfamonomethoxine by fourier transform infrared (FTIR). It is suggested that appearance of typical fanlike or wheat bunchy crystals in urinary sediments under observation of lightmicroscope and determination by FTIR for the crystals are helpful in diagnosing sulfa calculi causced swine urolithiasis.

Keywords: Calculi, pig, sulfamonomethoxine.

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707 Prediction of Dissolved Oxygen in Rivers Using a Wang-Mendel Method – Case Study of Au Sable River

Authors: Mahmoud R. Shaghaghian

Abstract:

Amount of dissolve oxygen in a river has a great direct affect on aquatic macroinvertebrates and this would influence on the region ecosystem indirectly. In this paper it is tried to predict dissolved oxygen in rivers by employing an easy Fuzzy Logic Modeling, Wang Mendel method. This model just uses previous records to estimate upcoming values. For this purpose daily and hourly records of eight stations in Au Sable watershed in Michigan, United States are employed for 12 years and 50 days period respectively. Calculations indicate that for long period prediction it is better to increase input intervals. But for filling missed data it is advisable to decrease the interval. Increasing partitioning of input and output features influence a little on accuracy but make the model too time consuming. Increment in number of input data also act like number of partitioning. Large amount of train data does not modify accuracy essentially, so, an optimum training length should be selected.

Keywords: Dissolved oxygen, Au Sable, fuzzy logic modeling, Wang Mendel.

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706 Mainland Chinese Customers' Intention toward Medical Tourism in Taiwan

Authors: Hsiu-Yuan Wang, Shwu-Huey Wang

Abstract:

This study proposes and tests a rescapturing elements of perceived gain and loss that, by perceived value of medical tourism products, influencintention of potential customers. Data from 301 usable qwere tested against the research model using the structmodeling approach. The results indicated that perceivedkey predictor of customer intentions. As for benefimedical quality, service quality and enjoyment wcomponents that significantly influenced the perceptiRegarding sacrifice, the effects of perceived risk on pewere significant. The findings can provide insights intohow destination countries can make medical tourism a wfor themselves and international patients.KeywordsMedical tourism, perceived valueintention.

Keywords: Medical tourism, perceived valueintention.

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705 Bifurcation and Chaos of the Memristor Circuit

Authors: Wang Zhulin, Min Fuhong, Peng Guangya, Wang Yaoda, Cao Yi

Abstract:

In this paper, a magnetron memristor model based on hyperbolic sine function is presented and the correctness proved by studying the trajectory of its voltage and current phase, and then a memristor chaotic system with the memristor model is presented. The phase trajectories and the bifurcation diagrams and Lyapunov exponent spectrum of the magnetron memristor system are plotted by numerical simulation, and the chaotic evolution with changing the parameters of the system is also given. The paper includes numerical simulations and mathematical model, which confirming that the system, has a wealth of dynamic behavior.

Keywords: Memristor, chaotic circuit, dynamical behavior, chaotic system.

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704 Transportation Under the Threat of Influenza

Authors: Yujun Zheng, Qin Song, Haihe Shi, and Jinyun Xue

Abstract:

There are a number of different cars for transferring hundreds of close contacts of swine influenza patients to hospital, and we need to carefully assign the passengers to those cars in order to minimize the risk of influenza spreading during transportation. The paper presents an approach to straightforward obtain the optimal solution of the relaxed problems, and develops two iterative improvement algorithms to effectively tackle the general problem.

Keywords: Influenza spread, discrete optimization, stationary point, iterative improvement

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703 Deep Learning Based 6D Pose Estimation for Bin-Picking Using 3D Point Clouds

Authors: Hesheng Wang, Haoyu Wang, Chungang Zhuang

Abstract:

Estimating the 6D pose of objects is a core step for robot bin-picking tasks. The problem is that various objects are usually randomly stacked with heavy occlusion in real applications. In this work, we propose a method to regress 6D poses by predicting three points for each object in the 3D point cloud through deep learning. To solve the ambiguity of symmetric pose, we propose a labeling method to help the network converge better. Based on the predicted pose, an iterative method is employed for pose optimization. In real-world experiments, our method outperforms the classical approach in both precision and recall.

Keywords: Pose estimation, deep learning, point cloud, bin-picking, 3D computer vision.

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702 MiR-200a/ZEB1 Pathway in Liver Fibrogenesis of Biliary Atresia

Authors: Hai-Ying Liu, Yi-Hao Chen, Shu-Yin Pang, Feng-Hua Wang, Xiao-Fang Peng, Li-Yuan Yang, Zheng-Rong Chen, Yi Chen, Bing Zhu

Abstract:

Objective: Biliary atresia (BA) is characterized by progressive liver fibrosis. Epithelial-mesenchymal transition (EMT) has been implicated as a key mechanism in the pathogenesis of organ fibrosis. MiR-200a has been shown to repress EMT. We aim to explore the role of miR-200a in the fibrogenesis of BA. Methods: We obtained the plasma samples and liver samples from patients with BA or controls to examine the role of miR-200a. Histological liver fibrosis was assessed using the Ishak fibrosis scores. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed to detect the expression of miR-200a in plasma. We also evaluated the expression of miR-200a in liver tissues using tyramide signal amplification fluorescence in situ hybridization (TSA-FISH). The expression of EMT related proteins zinc finger E-box-binding homeobox 1 (ZEB1), E-cadherin and α-smooth muscle actin (α-SMA) in the liver sections were detected by immunohistochemical staining. Results: We found that the expression of miR-200a was both elevated in the plasma and liver tissues from BA patients compared with the controls. The hepatic expression of ZEB1 and α-SMA were markedly increased in the liver sections from BA patients compared to the controls, whereas E-cadherin was downregulated in the BA group. Simultaneously, we noted that the hepatic expression of miR-200a, E-cadherin and α-SMA were upregulated with the progression of liver fibrosis in the BA group, while ZEB1 was downregulated with the progression of liver fibrosis in BA patients. Conclusion: These findings suggest EMT has a critical effect on the fibrotic process of BA, and the interaction between miR-200a and ZEB1 may regulate EMT and eventually influence liver fibrogenesis of BA.

Keywords: Biliary atresia, liver fibrosis, MicroRNA, epithelial-mesenchymal transition, zinc finger e-box-binding homeobox 1.

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