World Academy of Science, Engineering and Technology

Authors: S. Candio, B. Louis Charles, L. Soares Santos

Abstract:

Cocoa butter is an essential ingredient in the production of various food and cosmetic products. Its physicochemical properties, particularly the refractive index and acidity index, play a crucial role in determining the quality and stability of the final products. Cocoa varieties, such as PS1319, BN34, and CCN51, exhibit genetic and agronomic differences that may influence these parameters. This study aims to compare these two indices across three cocoa butter varieties to better understand the varietal influences on cocoa butter properties. The refractive index was measured at 60 °C using a digital refractometer, following the AOCS method, and the acidity index was determined by titration with a 0.1 M NaOH solution after dissolving 2 grams of cocoa butter in an ether-alcohol (2:1) solution and adding two drops of phenolphthalein as an indicator. Statistical analysis was conducted using SAS® OnDemand for Academics software. The results revealed similar refractive index values for the three varieties: PS1319 (1.4482), BN34 (1.4484), and CCN51 (1.4481), indicating a comparable lipid composition among them. On the other hand, the acidity index showed more pronounced differences: PS1319 (0.8759 mg KOH/g), BN34 (0.3381 mg KOH/g), and CCN51 (1.0899 mg KOH/g), although no statistically significant differences were detected at a 5% confidence level. These findings suggest uniformity regarding the refractive index but variability in the acidity index, likely due to minor differences in fatty acid composition among the varieties. These observations provide valuable insights for optimizing the quality of cocoa butter in food and cosmetic applications.

Keywords: BN34, CCN51, physicochemical properties, PS1319, quality

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Authors: Hamza Armghan Noushahi, Luis Inostroza, Viviana Barahona, Soledad Espinoza, Carlos Ovalleb, Katherine Quitral, Gustavo A. Lobos, Fernando Guerra, Shawn Kefauver, Alejandro del Pozo

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Introduction: One of the primary environmental factors affecting forage crop yield globally is drought, particularly in Mediterranean climatic conditions, where drought typically persists for 5-6 months, usually between October and March in countries like Chile. Alfalfa, a perennial forage crop with deep roots, employs a diverse range of drought-tolerant strategies at the physiological, morphological, and molecular levels. In the current study, 250 alfalfa half-sib populations containing different genetic makeups were grown for three growing seasons (2021 to 2023) to identify drought-resistant populations with high forage yield in two water regimes (irrigated and rainfed) under the Mediterranean drought-prone region of Central Chile, Cauquenes. The objectives were to i) develop new field phenotyping methods using remote sensing technologies such as Red-Green-Blue (RGB) and thermal cameras to identify high-yielding and drought-tolerant alfalfa populations, and ii) select outstanding genetic material for plant breeding. Material And Methods: Field phenotyping involves using remote sensing technology, including RGB and thermal cameras mounted on unmanned aerial vehicles, and measuring the forage yield of 250 alfalfa half-sib populations grown under rainfed and irrigated water regimes in a Mediterranean drought-prone environment, during three growing seasons (2021-2023). Both trials were arranged in an α-lattice experimental design with two replications. Each replicate has 10 partial blocks including 25 half-sib populations. RGB-derived indices and canopy temperature difference (CTD), determined by subtracting the canopy temperature (Tc) from the ambient temperature (Ta), were related with forage yield. Results And Discussion: Results indicate that forage yield exhibited significant variability among the alfalfa populations, in both rainfed and irrigated conditions. During winter, it ranged from 1.4- to 6.1 Mg ha-1 in rainfed conditions and from 1.4 to 8.2 Mg ha-1 under the irrigated regime. Total forage yield ranged from 3.7 to 14.7 Mg ha-1 in rainfed conditions and from 6.3 to 25.1 Mg ha-1 in the irrigated regime. Among half-sib populations, the most productive populations were AlfaL4-5 (parent SARDI7), AlfaL57-7 (parent WL903), and AlfaL62-9 (parent Baldrich350), which produced the highest (>13 Mg ha-1 mean total FY and > 4.5 Mg ha-1 mean winter FY during 2021-2023) forage yield in both water regimes. RGB indices Hue, Saturation, b*, v*, GA, and GGA exhibited positive correlations, whereas Intensity, Lightness, a*, and u* showed negative correlations with forage yield in both water regimes. In 2021, RGB-derived indices showed a weak correlation (r < 0.5) with CTD. However, strong correlations were observed in November 2022 (r = -0.8 to +0.8) and 2023 (r = -0.7 to +0.7), specifically in the irrigated regime, indicating better performance under higher water availability. Moreover, the CTD was negatively correlated with FY (r = -0.28 for rainfed and -0.32 for irrigated in 2021, r = -0.57 for rainfed and r = -0.76 for irrigated in 2022, and r = -0.34 for rainfed and r = -0.52 for irrigated in 2023) of 250 alfalfa half-sib populations. It is concluded that CTD and RGB-derived indices were the most effective tools for identifying drought-resistant populations grown in Mediterranean drought-prone environments. In rainfed alfalfa, the most highly productive populations were AlfaL29-4 (parent AS3), AlfaL61-9 (parent Genesis), and AlfaL4-7 (parent SARDI7). Meanwhile, in irrigated conditions, the alfalfa half-sib populations AlfaL56-4 (parent Venus) and AlfaL57-2 (parent WL903) demonstrated maximum FY. Conclusion: Alfalfa winter and total FY varied widely between the three growing seasons (2021-2023) under two water regimes, rainfed and irrigated. There were three alfalfa half-sib populations, AlfaL4-5 (parent SARDI7), AlfaL57-7 (parent WL903) and AlfaL62-9 (parent Baldrich350), that exhibited high FY in both water regimes, rainfed and irrigated. The thermal camera derived index CTD (Tc-Ta) showed negative correlation with FY and appeared to be the most powerful tool in identification of alfalfa genotypes grown under Chilean Mediterranean drought prone environment.

Keywords: alfalfa, remote sensing, phenotyping, forage crop

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Authors: Yongjun Zeng, Shan Huang, Guanjun Huang, Changzhi Long

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The chalkiness, starch fine structure, and physiochemical properties of rice starch were analyzed, and their correlations were investigated under different nighttime temperatures during the early grain-filling stage. Compared to MT, medium temperature (MT) and low (LNT) and high (HNT) nighttime temperatures resulted in an increased chalky grain rate (CGR) and chalkiness degree (CD). LNT mainly affected the chalkiness by increasing peak1 (short branch chains of amylopectin), the branching degree, and the proportion of small starch granules but decreasing peak2 (long branch chains of amylopectin) and peak 3 (amylose branches). This altered the pasting properties, such as by increasing the peak viscosity and final viscosity. However, HNT mainly affected the chalkiness by increasing peak 2 and the crystalline degree but decreasing peak 1 and peak 3. Regarding the thermal properties, HNT also elevated peak and conclusion temperatures. The CGR and CD were significantly and positively correlated with the proportions of small and medium starch granules, peak1, branching degree, gelatinization enthalpy, setback viscosity, and pasting time but markedly and negatively correlated with the proportion of large starch granules, amylose content, peak 3, peak viscosity, and breakdown viscosity. These findings suggest that LNT and HNT disrupted the starch structure, resulting in increased chalkiness. However, their mechanisms of action differ.

Keywords: chalkiness, starch fine structure, different nighttime temperatures, early grain-filling stage

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Authors: Guanjun Huang, Shan Huang, Yongjun Zeng, Jiaojiao Wu

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Improving the yield and quality of tasty rice varieties is a great challenge. In the present study, different nitrogen rates and plant densities were utilized to form differential rice populational structures, which were determined to clarify key traits determining grain yield and quality for tasty rice varieties in a double-cropped rice system in subtropical China. The present results showed that the plant photosynthetic rate, leaf area index and plant respiration rate had important and significant impacts on the grain yields of both early and late rice, though the late rice yield was also significantly affected by the canopy temperature. In addition, among the studied populational traits, plant photosynthetic and/or respiration rates had significant effects on all quality traits. Consistently, grain yield and quality were significantly improved with the increasing plant photosynthetic and respiration rates through correlative analysis, which was also observed in principal components analysis. Overall, the present study suggests that both the grain yield and milling and appearance qualities could be improved through the optimal management of nitrogen and plant density through increasing plant photosynthetic and respiration rates.

Keywords: rice yield, rice quality, plant photosynthetic rate, plant respiration rate

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Authors: Salihu Umaru Biye, Ali Disa, Y. Adamu, Muhammad Elhafiz Ahmad

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This study evaluated the economic efficiency and profitability of cowpea production in Billiri Local Government Area of Gombe State, Nigeria. The objectives were to describe the socioeconomic characteristics of cowpea farmers, analyze the costs and returns of production, determine technical and allocative efficiencies, and identify constraints to cowpea farming. Using multistage, purposive, and simple random sampling techniques, we selected 200 cowpea farmers. Data were collected through structured questionnaires and analyzed using descriptive and inferential statistics. Results indicated that 76% of farmers were under 45 years old, with a mean age of 36.3 years. The majority (74%) were male, 73% married, and had an average family size of 8. About 46% were full-time farmers, 95.5% were literate, with an average farming experience of 15.45 years and an average farm size of 3.08 hectares. Cowpea production proved profitable with a gross margin of ₦326,740.25 per hectare, a gross income of ₦525,020.00 per hectare, and total variable costs of ₦198,279.75 per hectare, resulting in an operating ratio of 0.61. The return on investment was 2.21, with a mean technical efficiency of 0.75 and a mean economic efficiency of 0.71. The findings suggest that cowpea production is profitable, yielding ₦2.21 for every ₦1.00 invested. Enhancing farming practices could further improve efficiency and profitability.

Keywords: economic efficiency, profitability, cowpea production, technical efficiency, allocative efficiency, Gombe State

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Authors: Mohamed Sami Albordini, Faris Tarlochan

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Bruises in apples affect the quality and acceptance of the apples and consumers’ willingness to accept apples after harvest, transport, and storage. This paper seeks to analyze the impact of various drop heights on bruising when apples are dropped on structural steel, a material that is frequently used in fruit pallets. In this study, 27 simulations were carried out using SolidWorks at three drop heights, including 500 mm, 1000 mm, and 1500 mm, and nine types of trays to determine the stress. The results reveal that the level of bruising rises with the increase in drop height and that stress impact velocity and surface hardness are decisive for it. Among the nine designs of the tray, tray design 1 was the best one in terms of the least stress measured at different drop heights. The findings imply the desirability of minimizing drop height and increasing packaging to reduce mechanical damage, increase apple quality, and increase shelf life.

Keywords: bruising, apple, drop height, shelf life, SolidWorks

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Authors: Marek Gancarz, Katarzyna Grądecka-Jakubowska

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The aim of the study was to analyze the cooling process of coffee beans after roasting in order to determine their mechanical strength. Arabica coffee beans came from two varieties of coffee: Brazil Diamantina and Ethiopia Refisha. Coffee beans were cooled with air or liquid nitrogen. The mechanical properties of roasted and then cooled coffee beans were determined using an INSTRON machine. It is equipped with a measuring head and a cylindrical probe that destroys the sample. The maximum force of the head used is 250 N. The process of cooling coffee beans using air was characterized by the lower mechanical strength of coffee beans compared to cooling with liquid nitrogen.

Keywords: coffee beans, cooling process, liquid nitrogen, mechanical properties

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Authors: Katarzyna Grądecka-Jakubowska, Rusinek Robert, Marek Gancarz

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The aim of the study was to analyze the process of roasting coffee beans in a convection–conduction roaster (CC) without a heat exchanger and a convection–conduction–radiation roaster (CCR) with a heat exchanger for determination of the colour of the coffee beans and coffee colour after mill. Arabica coffee from the following countries (regions) was used for the study: (1) Ethiopia Refisha, (2) Guatemala Santa Barbara, (3) Honduras El Puente, (4) Kenya Baragwi, (5) Brazil Beyond. The coffee beans were roasted using two types of roasters: convection–conduction roaster (CC) without a heat exchanger and a convection–conduction–radiation roaster (CCR) with a heat exchanger. The analysis of the color of coffee beans and ground coffee was carried out using the CIELab and RGB method using a Lovibond CAM-System 500 colorimeter (Great Britain). The device allows you to evaluate the color and record the image in a resolution of 752 × 582 pixels, saving each pixel as an RGB component. The time profile screen captured a sequence of images at fixed time intervals and displayed them on-line. The system, useful for assessing non-uniform or variable colors, allowed us to record the entire image or appropriate areas (surfaces) of the sample. Color is mathematically described by three components: L - lightness (luminance from 0 very to 100 very bright), (a) - color from green to magenta (from -120 to +120), (b) - color from blue to yellow (from -120 to +120). Coffee beans roasted in the Dietrich (CCR) roaster had a lighter colour, while those roasted in the Gothot (CC) roaster had a darker colour. In the case of ground coffee colour tests, coffee ground from beans roasted in the Dietrich (CCR) roaster also had a lighter colour, while coffee ground from beans roasted in the Gothot (CC) roaster had a darker colour.

Keywords: coffee beans, ground coffee, colour, CIELab, RGB

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Authors: Michael Adegoke, Kenichi Matsui

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Cocoa production in Ghana encounters several challenges, including socioeconomic constraints, climate change impacts, limited global market access, and increasing demand for high-quality cocoa. It has also experienced deforestation, soil degradation, low productivity, pest and disease outbreaks, and fluctuating global market prices. These challenges persist despite concerted efforts by the Food and Agriculture Organization (FAO), Ghana’s Ministry of Food and Agriculture (MoFA), and the Ghana Cocoa Board (COCOBOD) that have provided technical, financial, and economic support to cocoa farmers. This study aims to assess cocoa farmers’ perceptions of environmental sustainability challenges in cocoa production in Ghana. A systematic review of peer-reviewed articles published between 2010 and 2024 was conducted using JSTOR and ScienceDirect databases. Of 1,045 articles initially retrieved, 25 articles meeting well-defined inclusion criteria were selected for analysis. The study adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to identify recurring themes and insights related to environmental sustainability in cocoa farming. Findings indicate that education and age significantly influence farmer’s perceptions of environmental sustainability. A major barrier to sustainable practices is the limited awareness of climate change impacts and the environmental consequences of unsustainable farming. Climate change has led to yield fluctuations and increased susceptibility to pests and diseases. Declining yields due to aging and diseased trees have prompted the Ghanaian government to implement large-scale rehabilitation and replanting programs, providing farmers with improved planting materials, pesticides, and fertilizers. Adaptation strategies such as agroforestry, improved cocoa varieties, and crop diversification show potential for mitigating these challenges. However, the current conditions for sustainable cocoa production remain insufficient. Structural reforms in the cocoa sector are necessary to enhance the economic viability of smallholder farms, promote efficient land management, and reduce the environmental impact of farming practices. This study concludes that area-specific programs targeting these structural changes are essential for achieving socially, economically, and environmentally sustainable cocoa production on less land.

Keywords: cocoa production, environmental sustainability, sustainable agriculture, climate change, Ghana

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Authors: Duwage C. Perera, Ravisha N. Mudalige, Jay N. Meegoda

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Per- and polyfluoroalkyl substances (PFAS), often referred to as "forever chemicals," are a class of environmentally persistent pollutants known for their exceptional chemical stability and resistance to conventional degradation methods. Among the various PFAS compounds, perfluorooctanoic acid (PFOA) has emerged as a priority contaminant due to its widespread occurrence, bioaccumulative nature, and toxicological effects on human health and ecosystems. The need for effective remediation strategies has driven significant interest in understanding the interactions between PFOA and potential adsorbent materials such as soils and sediments at the molecular level. In this study, density functional theory (DFT) is employed to investigate the adsorption mechanisms of PFOA on kaolinite, a naturally abundant clay mineral with promising applications in PFAS remediation. The computational approach involves constructing atomistic models of the kaolinite (001) surface to capture its unique structural and chemical characteristics. Both the tetrahedral (Si-O) and octahedral (Al-O) layers of kaolinite are included in the models, with varying degrees of surface hydroxylation to simulate environmentally relevant conditions. PFOA is modeled in both protonated and deprotonated states, reflecting its behavior under different pH levels commonly encountered in natural and engineered systems. The adsorption energies are calculated to quantify the affinity of PFOA for kaolinite, while Bader charge analysis is conducted to examine charge redistribution and electrostatic interactions during the adsorption process. A detailed investigation of the molecular interactions between PFOA and kaolinite reveals the critical role of hydrogen bonding, van der Waals forces, and electrostatic interactions in PFOA adsorption. The carboxylic group of PFOA demonstrates strong binding to hydroxylated sites on the kaolinite surface, while the hydrophobic tail of PFOA interacts minimally with the mineral, reflecting its dual hydrophilic-hydrophobic nature. Vibrational frequency analysis is performed to identify shifts in the functional group vibrations, providing additional evidence of strong chemical interactions between PFOA and kaolinite. This study provides a comprehensive understanding of the molecular-level interactions governing PFOA adsorption on kaolinite. By elucidating the underlying mechanisms, it establishes a foundation for the design and optimization of clay-based remediation technologies aimed at mitigating PFAS contamination in environmental systems. Future work integrating experimental validation with the computational insights presented here will further enhance the applicability of kaolinite and other clay minerals in PFAS remediation efforts, addressing a critical global environmental challenge.

Keywords: adsorption mechanism, carbon-fluorine bond stability, density functional theory, kaolinite adsorption, perfluorooctanoic acid, PFAS, soil and water contamination

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Authors: Ravisha N. Mudalige, Duwage C. Perera, Jay N. Meegoda

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Perfluorooctanoic acid (PFOA), a persistent and hazardous member of the per- and polyfluoroalkyl substances (PFAS) family, presents significant environmental challenges due to its exceptional durability, potential for bioaccumulation, and mobility in natural systems. As a "forever chemical," PFOA resists degradation, resulting in widespread contamination of soils and sediments. This study investigates the molecular-level mechanisms governing the adsorption of PFOA on two negatively charged clay minerals, kaolinite, and montmorillonite, under the influence of humic acid. Adsorption behavior is analyzed using the Langmuir isotherm model under two conditions: humic acid-coated clay to mimic organic substances and non-coated clay. The study also examines the effects of pH levels of 2 and 7, focusing on the role of protonation states, clay surface characteristics, and solution chemistry in influencing adsorption dynamics. Humic acid, an organic substance formed from the decomposition of plant and animal matter, significantly influences the surface properties of clay particles. By altering surface charge, increasing hydrophobicity, and providing additional binding sites, it enhances the clays' ability to interact with PFOA. Typically, the negatively charged surfaces of kaolinite and montmorillonite repel the equally negatively charged PFOA molecules, creating electrostatic repulsion that limits direct adsorption. However, the cation exchange capacity (CEC) of these clays is a pivotal factor that allows them to retain positively charged species, such as metal ions or functional groups introduced by humic acid coatings. These positively charged components act as intermediaries, bridging electrostatic interactions and facilitating hydrophobic partitioning, ultimately increasing the adsorption efficiency of PFOA onto the clay surfaces. At pH 2, increased protonation of the clay surfaces reduces electrostatic repulsion, enhancing PFOA adsorption, while humic acid coatings provide additional binding sites due to hydrophobicity. Conversely, at pH 7, adsorption is reduced due to dominant electrostatic repulsion, lower surface protonation, and competition between PFOA and humic acid components for available adsorption sites. This study provides molecular-level insights into the critical roles of clay chemistry, CEC, organic matter, and interfacial dynamics in overcoming electrostatic barriers to PFOA adsorption. By highlighting the essential role of organic matter in overcoming electrostatic repulsion, this work contributes to the development of more effective strategies for mitigating PFAS contamination in soils and water systems, offering valuable guidance for environmental remediation efforts.

Keywords: adsorption, clay surface, humic acid, Langmuir isotherm, prfluorooctanoic acid, PFAS

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Authors: Kuo-Dung Chiou, Yen-Xue Chen, Chia-Ying Chang

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This study uses AI technology to establish an expert system and establish a fruit appearance database for pineapples and custard apples. It collects images based on appearance defects and fruit maturity. It uses deep learning to detect the location of the fruit and can detect the appearance of the fruit in real-time. Flaws and maturity. In addition, a hyperspectral camera was used to scan pineapples and custard apples, and the light reflection at different frequency bands was used to find the key frequency band for pectin softening in post-ripe fruits. Conducted a large number of multispectral image collection and data analysis to establish a database of Pineapple Custard Apple and Big Eyed Custard Apple, which includes a high-definition color image database, a hyperspectral database in the 377~1020 nm frequency band, and five frequency band images (450, 500, 670, 720, 800nm) multispectral database, which collects 4896 images and manually labeled ground truth; 26 hyperspectral pineapple custard apple fruits (520 images each); multispectral custard apple 168 fruits (5 images each). Using the color image database to train deep learning Yolo v4's pre-training network architecture and adding the training weights established by the fruit database, real-time detection performance is achieved, and the recognition rate reaches over 97.96%. We also used multispectral to take a large number of continuous shots and calculated the difference and average ratio of the fruit in the 670 and 720nm frequency bands. They all have the same trend. The value increases until maturity, and the value will decrease after maturity. Subsequently, the sub-bands will be added to analyze further the numerical analysis of sugar content and moisture, and the absolute value of maturity and the data curve of maturity will be found.

Keywords: hyperspectral image, fruit firmness, deep learning, automatic detection, automatic measurement, intelligent labor saving

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Authors: Adepoju A. A., Raufu M. O., Ganiyu M. O., Olawuyi S. O., Olalere J. O., Ogunkunle A. A.

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This study investigated the effects of social safety nets on food security among farming households in Southwest Nigeria. The study used a multistage sampling technique, purposively selecting two states from southwest Nigeria, Oyo and Ogun as the study area with eight Agricultural Development Programme (ADP) agricultural zones. The Local Government Areas (LGAs) were stratified into urban and rural LGAs. Sixteen villages from Oyo and 12 villages from Ogun were randomly selected from the rural LGAs using a proportionate to-size sampling, resulting in 472 respondents, with 271 and 201 from Oyo and Ogun states, respectively. The data was analyzed using descriptive statistics like mean, standard deviation, frequency and percentages, while logistic regression analysis examines the association between independent variables and dependent variables. The study found that poverty reduction, social empowerment, food security palliative, microcredit, and agricultural empowerment are the most prevalent social safety nets among farming households. School feed programs are the most prevalent form of poverty reduction, while training for empowerment improves wellbeing. Food item distribution is the most beneficial for food security and wellbeing. Self-empowerment-based micro-credit support is the most effective, while Anchor Borrower's project is the most beneficial for agricultural empowerment. The study found that 62.68% of the variance in food security status is explained by independent variables. females farmers have a 56% higher likelihood of being food secure than their male counterparts. An additional increase in age decreases the likelihood of being food secure by 6%. Married individuals have a 58% lower likelihood of being food secure compared to singles, possibly due to increased financial responsibilities. A larger household size increases the likelihood of being food secure by 3.41%. Larger households may benefit from economies of scale or shared resources and social safety net programs. Engagement in farming as a primary occupation increases the likelihood of being food secure by 62%. The study further reveals that participation in poverty reduction and microcredit programs significantly increases the likelihood of food security by 30,069% and 135.48%, respectively. The study therefore recommends expanding school feed programs, improving empowerment training, strengthening food distribution, promoting micro-credit, supporting agricultural empowerment, and addressing gender disparities in social safety net programs.

Keywords: poverty reduction, food distribution, micro-credit, household well-being

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Authors: Ahmed Ibrahim Galadima, Abdullahi Muhammed Bello, Abdullateef Akintunde Raji, Ruqayyah Mujahidah Abdullateef, Hajara Sani Labaran

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Sesamum indicium is an erect, herbaceous, branched, and warm-season annual crop. It differs in growth habit, form and shape, seed size and color, composition, size, and color of flowers. The experiments were performed by collecting two accessions of Sesamum indicum (white and black varieties). The morphological parameters like plant height and leaf size were measured using a ruler, while stem girth was observed using a vernier caliper. Germinability assessments were conducted by placing 100 seeds from each of the two sesame varieties and presoaked in 5 per cent sodium hypochlorite for 15 min., followed by rinse with distilled water. Germinability was assessed by placing the seeds in moist conditions and analyzing the outcome accordingly. The results from the experiments showed that the plant height (41.98 cm), stem girth (3.23 cm), and size of leaves (13.89 cm2) were higher in the white sesame variety when compared to the sesame black variety where 34.30 cm, 2.68 cm, and 7.47 cm2, respectively were obtained. But, from the black sesame variety, the number of leaves and the number of branches high values (105.06 cm) and (10.41), respectively were discovered. And significant differences were recorded in all the analyzed morphological parameters between the two sesame accessions, except stem girth. More so, the white variety of the sesame plant has demonstrated a good reproductive capability with the number of flowers (7.18), number of capsules (17.44), 100 seeds weight (1.67 g), and 1000 seeds weight (16.67 g). However, none of the analyzed reproductive parameters was observed from the black sesame variety. The white sesame variety was observed to have a higher number of Trichomes per microscopic view by almost 7 folds when compared to the black variety. But, improved size of stomata was detected in the black sesame variety by over 1.5 folds compared to the white variety. The germinability analysis had shown that from the sesame white variety, 98 seeds out of the 100 seeds sown were able to germinate, which signifies 98 percent germinability. Whereas 86 seeds out of the 100 seeds sown have germinated in petri dishes from the black sesame variety, which suggests 86 per cent germinability. Therefore, this research obviously showed that the white sesame variety displayed remarkable morphological qualities and, accordingly could be employed for farming purposes.

Keywords: accessions of sesame plant, characterization, growth, morphology

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Authors: Musa Umar Tanimu, Adamu Muhammad, Ibrahim Umar Mohammed

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Two field trials were conducted to study the performance of transplanted rice under the influence of weed management practice and the age of seedlings at the teaching and research farm of Kebbi State University of Science and Technology Aliero located at Jega during 2020/2021 and 2021/2022 dry seasons. Treatments consisted of three seedlings age (10, 17, and 24 days old) and weed management practice (comprising of three selected postemergence herbicides, namely: (i) Bracer (0.025, 0.027, 0.030, and 0.032kg a.i.ha-1), (ii) Bracerplus at 0.021, 0.023, 0.025, and 0.027kg a.i.ha-1, and (iii) Nomineegold (0.020, 0.030, 0.040 and 0.050 kg a.i.ha-1, (iv) Farmers’ practice (hoe weeding at 4 and 8 weeks after transplanting) and (v) weedy check. The treatments were laid out in a randomized complete block design (RCBD) in a split-plot arrangement with three replications. Results showed that the application of Bracerplus at 0.021 produced highest grain yield (4,448.85 kgha-1) and highest panicle weight (2.99g), while application of Bracer at 0.025 produced the highest 1000 grains weight (26.17), the application of Nomineegold produced the highest total number of grains per panicle (111.72), the younger (10-day-old) seedlings recorded higher grain yield over other seedlings (3488.25 kgha-1). In conclusion, the highest grain yield, 4,448.85kg ha-1 was 57.15% higher than the farmers' practice (weeding at 4 and 8 weeks after planting). Seedlings transplanted at 10-day old recorded the highest grain yield of 3,488.25kg ha-1; it was 21.43% higher than 24-day-old seedlings and 15.87% higher than 17-day-old seedlings. It is recommended that the application of Bracerplus at 0.021kg a.i. ha-1 should be used for higher grain yield, and seedlings at 10- days old should be used for transplanting from the nursery.

Keywords: bracerplus, nomineegold, rice, split-plot-arrangement, transplanted rice, yield components

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Authors: Flóra Karsai-Rektenwald, Khongorzul Odgerel, Vanda Villányi, Zoltán Gábor Tóth, Zsófia Bánfalvi

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GIGANTEA (GI) is a plant-specific, circadian clock-regulated, nuclear protein involved in diverse processes from flowering to stress responses. In the obligate short-day tuberising Andigenum Group potatoes, GI is indirectly involved in determination of the time of tuber initiation. The goal of our study was to get information on the function of GI in the day-length independent tuberising commercial potato cultivar ‘Désirée’, a tetraploid plant carrying two GI genes, one on chromosome 4 (GI.04) and another one on chromosome 12 (GI.12). Functional analysis of the two GI genes was attempted by targeted mutagenesis using the CRISPR-Cas9 system. Two sets of mutants were generated. The mutations were mapped at nucleotide level and the plants grown in a greenhouse. GI is located in the nucleus and interacts with at least five proteins. Three out of them, two photoreceptors and FKF1, bind on GI close to the nuclear localisation (NLS) signal to the so called LOV domain. In Andigenum Group potatoes, FKF1 interacts not only with GI but form a triplex with CDF1, a positive regulator of tuberisation, and transport it to proteasomes, where CDF1 is degraded. Three GI.04 and three GI.12 null mutants were selected from the first set of mutagenesis. Although, the deletions did not reach the NLS and LOV domain in any of the six mutants all GI. 04 and two GI.12 mutants were shorter than the control suggesting that both GIs are involved in vegetative growth regulation and the deleted region might be important in terms of conformation or stability of the proteins. From the second set of mutagenesis, three null mutants carrying mutations in one of the two GI genes and three mutants carrying mutations in both GI genes were selected for detailed analysis. Deletions in the GI mutants of this set disrupted the NLS and extended to the LOV domain. Nevertheless, none of the single GI gene mutations influenced the time of tuberisation or the tuber number and yield, whereas one of the GI.04 and all GI.12 mutants were shorter than the ‘Désirée’ control. Furthermore, all GI.12 mutants showed early senescence. The early senescence of mutants carrying mutations in both GI genes was even more pronounced, resulting in substantial yield loss in one of the double mutants. These results raise the possibility that the two GI genes can substitute each other in term of tuberisation or they are not involved in it, the yield loss is due to the early death of the plants. To distinguish between the two possibilities yeast two-hybrid experiments were initiated to detect the interaction between the GI proteins and FKF1 and between FKF1 and CDF1 originated from ‘Désirée’.

Keywords: gene editing, tuberisation, senescence, Solanum tuberosum

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Authors: Nouri Lilya, Zouagri Imen, Bezzalla Adel

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The aim of our present study is to develop a reliable experimental protocol for regenerating our species, which has multiple uses (medicinal, cosmetic .... etc.); to do this, we carried out micropropagation of the leaves and seeds of Argania spinosa L. We tested two different provenances; from Morocco and from Tindouf in three media MS, MS/2 and WPM with a fixed hormonal concentration (10ml 2,4 D; 3 ml kinetin). In fact, this study allowed us to conclude that Argan seeds have a good aptitude for callogenesis by obtaining different types of callus on the media tested, especially in the MS and MS/2 media, in comparison with the leaves (from Tindouf) which have a good aptitude for callogenesis in the WPM medium. As a result, callogenesis depends not only on the hormonal nature of the plant but also on the quality of the explant and its original variety. At the end of this study, it can be said that Argania spinosa L. from Tindouf is not resistant to in vitro culture methods, which means that it can be regenerated by micropropagation and is more reactive than the Moroccan varieties.

Keywords: Argania spinosa L., in vitro culture, regeneration, MS, MS ½, WPM, callogenesis

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Authors: Khater Nadia, Garah Kenza

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Moringa stenopetala is a rapidly growing, unappreciated tree regarded as the "miracle tree" for its food, feed, and medicinal benefits. It appears to be a versatile and promising species for use under changing conditions. To evaluate the effect of water stress on germination seeds of M. stenopetala, three different concentrations PEG- 6000 (4, 8, and 12 per cent) along with a control in a factorial experiment based on a completely randomized design with five replications. The results revealed that water potential significantly reduced germination rate (82.5%) and average germination time. Germination speed in T3 by 93%, kinetics germination in T2 (39), germination index in T2 (102) and germination vigor index in T2 (91.25) were increased in the osmotic potential of PEG solution. By following these steps, we can improve the chances of successful germination of M. stenopetala seeds under water stress conditions

Keywords: moringa stenopetala, PEG, water stress, rate

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Authors: Reza Sadeghi, Maryam Nazarahari

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Considering the effectiveness of botanical pesticides in pest management, these compounds have garnered attention as a sustainable approach to reducing pest-induced damage in agriculture while preserving the environment. Botanical pesticides enable farmers to cultivate higher-quality crops by minimizing the use of chemical pesticides. In this study, plant extracts obtained using n-hexane as a solvent from two botanical sources, thyme and eucalyptus, were evaluated under laboratory conditions for their effectiveness in controlling the cotton bollworm (Helicoverpa armigera). The mortality rate of bollworm larvae was assessed across various concentrations of the hexane-based formulations. The results revealed that the hexane-based formulations of thyme and eucalyptus extracts significantly reduced the population of bollworm larvae after 24 hours of exposure. Thyme extract, in particular, demonstrated high effectiveness as a botanical pesticide, suggesting its potential as an efficient alternative to chemical pesticides in pest management. These findings underscore that botanical pesticides can mitigate the environmental consequences of chemical pesticides and provide innovative solutions for sustainable agriculture by leveraging the active compounds present in plant extracts.

Keywords: cotton bollworm, thyme, eucalyptus, extract formulation, , toxicity

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Authors: Dedie Tooy, Rio Kolibu, Rio Putra, Herry Frits Pinatik, Daniel P. M. Ludong

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This study presents the design and development of a smart greenhouse prototype tailored to optimize Pak choi (Brassica chinensis L.) cultivation in tropical rural climates. Pak choi, a high-demand leafy vegetable in Indonesia, often experiences suboptimal growth due to elevated temperatures and humidity. The objective of this research is to design and develop an intelligent greenhouse to optimize pak choi cultivation in tropical rural climates. The design of a smart greenhouse provides a controlled environment to stabilize these conditions, but managing fluctuating temperature, humidity, and light in tropical regions remains challenging. This system regulates critical environmental factors, including temperature, humidity, irrigation system, and light, creating optimal conditions for Pak Choi. The prototype's effectiveness was evaluated by monitoring growth indicators such as leaf weight, freshness, and moisture content, alongside the consistency of the internal climate compared to external conditions. Results indicate that the smart greenhouse supports superior crop growth, enhances yield quality, and reduces environmental resource consumption. The irrigation control system test was carried out for 40 days. Researchers observed the results of the automatic system working according to the sensor value readings. The results of the temperature control system test work: when the air temperature in the greenhouse is more than 33 degrees, the condensation pump will turn on, and when the temperature is below 32 degrees, the pump will automatically turn itself off. The cycle repeats continuously. The results achieved pak coy can live up to 40 days. As part of our ongoing research, we are actively considering integrating double-layered roofs to improve insulation and reduce external temperature fluctuations, which could further enhance the effectiveness of the smart greenhouse.

Keywords: smart greenhouse, horticulture, rural tropical climate, sustainable agriculture

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Authors: Nazanin Nikanmajd

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Microorganisms are essential for influencing environmental processes, such as nutrient cycling, pollutant breakdown, and ecosystem well-being. Recent developments in high-throughput sequencing technologies and metagenomic methods have given us fresh understandings about the range and capabilities of microorganisms in different settings. This research examines how environmental stressors like climate change, pollution, and habitat degradation affect the composition and roles of microbial communities in soil and water ecosystems. We show that human-caused disruptions change the makeup of microbial communities, causing changes in important metabolic pathways for biogeochemical processes. More precisely, we pinpoint important microbial groups that show resistance or susceptibility to certain stress factors, emphasizing their possible uses in bioremediation and ecosystem rehabilitation. The results highlight the importance of adopting a holistic approach to comprehend microbial changes in evolving environments, impacting sustainable environmental conservation and management strategies. This research helps develop new solutions to reduce the impacts of environmental degradation on microbial ecosystem services by understanding the intricate relationships between microorganisms and their surroundings.

Keywords: environmental microbiology, microbial communities, climate change, pollution, bioremediation, metagenomics, ecosystem services, ecosystem restoration

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Authors: Mohammad Abdul Momin, Sekender Ali, Mahbubul Alam, Rafiquel Islam, Mohammad Mizanul Haque Kazal

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Improved high-yielding modern rice varieties can reduce hunger and food insecurity in Bangladesh. However, lower adoption and higher adoption gap of modern rice varieties are the main concerns of rice researchers, extension specialists, and legislators. This study attempts to determine the adoption status and adoption gap of 10 selected BRRI-released Boro rice varieties to assess some selected socio-economic characteristics of the rice farmers and to explore the contribution of the selected socio-economic characteristics of farmers to their adoption gap of selected BRRI-released Boro varieties. Necessary data were collected from 03 September to 31 December 2021 using a well-structured pre-tested interview schedule from 371 randomly selected farmers covering 12 agricultural blocks of four Upazilas under Cumilla, Mymensingh, Tangail, and Bogura districts. The study revealed that most (73.05%) of the rice farmers had high adoption and low adoption gap; 23.72% had moderate adoption and adoption gap; and the rest 3.23% of respondents’ farmers had low adoption and high adoption gap of BRRI-released Boro rice varieties. Overall adoption and adoption gap of BRRI-released Bororice varieties were 77.02% and 22.98%, respectively. Based on the descending order of the Adoption Index, BRRI dhan29 ranked 1st, followed by BRRI dhan28. The adoption indices of these two top-ranked varieties were 38.84 and 30.43, respectively, which were much higher than others. Third to ninth ranked varieties were BRRI dhan58, BRRI dhan89, BRRI dhan88, BRRI dhan50, BRRI dhan74, BRRI dhan81, and BRRI dhan63. Reverse-ranked orders were observed based on the descending order of the Adoption Gap Index (AGI). Stepwise multiple regression analysis indicated that ‘knowledge on BRRI-released Boro rice varieties’, ‘extension contacts’, ‘rice farming profitability’, ‘rice farming experience’, and ‘satisfaction on BRRI-releasedBoro rice varieties’ of the farmers had a significant negative contribution to their adoption gap, i.e., positive contribution to their adoption of BRRI-released Boro rice varieties. The study concluded that policy interventions should be taken to improve farmers’ knowledge of BRRI-releasedBoro rice varieties by increasing extension contact to all the lower and higher experienced farmers to make them profitable and satisfied to increase adoption and decrease the adoption gap of BRRI-released Boro rice varieties. These issues also urge policy interventions for the rethinking of current dissemination tactics to ensure the widespread adoption of newly released modern Boro rice varieties at the farm level.

Keywords: adoption, adoption gap, Boro, rice, BRRI, Bangladesh

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Authors: Denis Constantin Topa, Irina Gabriela Cara, Gerard Jitareanu

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Tillage is a fundamental soil practice with different soil disturbance intensities and unique implications in soil organic carbon, soil structure, and nutrient dynamics. However, the implication of tillage practice on soil organic carbon and soil health is complex and specific to the context. it study evaluated soil health status based on soil carbon sequestration and pools, soil aggregation, and nutrient use under two different tillage practices: conventional and minimum tillage. The results of our study are consistent with the hypothesis that, over time, minimum tillage typically boosts soil health in the 0-10 cm soil layer. Compared to the conventional practice (19.36 t C ha-1) there was a significant accumulation of soil organic carbon (0-30 cm) in the minimum-tillage practice (23.21 t C ha-1). Below 10 cm depth, the soil organic carbon stocks are close to that of the conventional layer (0-30 cm). Soil aggregate stability was improved under conservative tillage, due to soil carbon improvement which facilitated a greater volume of mesopores and micropores. Total nitrogen (TN), available potassium (AK) and phosphorus (AP) content in 0-10 cm depth under minimum-tillage practice were 26%, 6% and 32%, greater respectively, compared to the conventional treatment. Overall, the TN, AP and AK values decreased with depth within the soil profiles as a consequence of soil practice and minimum disturbance. The data show that minimum tillage is a sustainable and effective management practice that maintain soil health with soil carbon increase and efficient nutrient use.

Keywords: minimum tillage, conventional tillage, soil organic carbon, nutrients, soil aggregation, soil health

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Authors: Kongngern K., Homwonk C., Theerakulpisut P., Roytrakul R.

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Rice cultivation is crucial globally, especially in Asia. Soil salinity poses a significant challenge for agricultural lands. Understanding the expression patterns of different rice varieties under salt stress can provide insights for developing more salt-tolerant cultivars. This study aims to compare the expression patterns of two rice varieties, Thai moderately salt-tolerant rice (Leaung Anan) and high salt-tolerant rice (Pokkali), in response to salt stress. By analyzing protein expression, the research seeks to identify key proteins associated with salt tolerance in rice. The expression patterns of the two rice varieties under salt stress were analyzed using 1D-SDS-PAGE, NanoLC-MS/MS, and MEV software. These methods enabled the researchers to assess the differential expression of proteins in the leaf sheaths of the rice plants. These results indicate that the study identified 18 proteins, exhibited significantly different expression patterns between the two rice cultivars under salt stress. Notably, certain proteins, such as Os05g0364500 and pr1-like protein, showed contrasting expression profiles in the two varieties. The up-regulated proteins, predominantly observed in the salt-tolerant rice, may contribute to the survival of rice plants under salt stress and may provide valuable insights for breeding programs aiming to enhance salt tolerancein rice cultivars.

Keywords: mass spectrometry, proteomics, rice leaf sheaths, salt stress

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Authors: Adedokun Mathew Adewale, Ayandiran Samuel Kola, Adekunle Ibironke

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Pigeon pea (Cajanus cajan) seeds contain about 20–22 percent protein and appreciable amounts of essential amino acids and minerals. Hence, this study evaluated the blood profile of weaner rabbits fed Cajanus cajan meal (CCM) as a replacement for groundnut cake. Forty weaner rabbits of mixed breed aged 5 - 6 weeks were used for the study, which lasted for 8 weeks. The rabbits were randomly allocated to four treatments (10 rabbits per treatment) in a completely randomized design. Four concentrate diets were compounded by direct replacement of groundnut cake with Cajanus cajan meal (CCM) at 0, 50, 75, and 100%, respectively. There were no significant differences (p>0.05) among the mean counts of packed cell volume, red blood cell, haemoglobin, and monocyte. The 75% CCM diet had significantly the highest (p<0.05). However, rabbits fed diets containing CCM had significantly higher (p<0.05) eosinophil than 0%CCM. Rabbits fed diets containing 100%CCM had significantly highest (p<0.05) total protein followed by 0%CCM, 75%CCM, and least 50%CCM, while 0%CCM and 75%CCM diets were significantly higher (p<0.05) in albumin. However, animals fed diets containing CCM had significantly lower (p<0.05) cholesterol content than 0%CCM diet. It could be concluded that Cajanus cajan meal could replace groundnut cake up to 100% in the diets of rabbits without any deleterious effect on the blood profile of the animals.

Keywords: blood profile, groundnut cake, pigeon pea, weaner rabbits

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Authors: Reza Sadeghi

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Storage pests with quantitative, qualitative, and hygienic losses to storage products lead to heavy damage to these products. One of the best methods of controlling storage pests is microwave heating, which is an environmentally friendly method and can be used to replace chemical methods to control storage pests. Pistachios and almonds are the most important dried fruit items in Iran, which account for a significant part of Iran's exports every year. In this study, which along with Pistachio and almond samples were exposed to microwave radiation at 320, 720, 900 watts with times of 10, 20, 30 seconds. Qualitative evaluation of product changes due to the above treatments was performed in the form of changes in colorimetric factors and organoleptic properties of the product. The results showed that in microwave treatment, power, and time factors had a significant effect on the taste and overall acceptance of pistachio product, polymer and power interaction, polymer and time, time and power had no significant effect on pistachio product quality. In almond products, the factors of polymer, time, power, interaction of polymer and power, polymer and time, and power had no significant effect on almond quality.

Keywords: microwave, qualitative, pistachio, almond

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Authors: Khizar Hayat Bhatti, Fiza Javed, Misbah Zafar

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Oat (Avena sativa L.) is a major cereal plant belonging to the family Poaceae. It is a very important source of carbohydrates, starch, minerals, vitamins and proteins that are beneficial for general health. Plants grow in the heavy metals contaminated soils that results in decline in growth. Glycine betaine application may improve plant growth, survival and resistance to metabolic disturbances due to stresses. Heavy metals, like nickels, have been accumulated for a long time in the soil because of industrial waste and sewage. The experiment was intended to alleviate the detrimental effects of heavy metal nickel stress on two oat varieties ‘Sgd-2011 and Hay’ using Glycine betain. Nickel was induced through soil application while GB was applied as foliar spray. After 10 days of nickel treatment, an exogenous spray of glycine betaine on the intact plant leaves. Data analysis was carried out using a Completely Randomized Design (CRD) with three replications in this study. For the analysis of all the data of the current research, Mini-Tab 19 software was used to compare the mean value of all treatments and Microsoft Excel software for generating the bars graphs. Significant accelerated plant growth was recorded when Ni exposed plants were treated with GB. Based on data findings, 3mM GB caused significant recovery from Ni stress doses. Overall results also demonstrated that the sgd-2011 variety of oats had the greatest outcomes for all parameters.

Keywords: CRD, foliar spray method, glycine betaine, heavy metals, nickel, ROS

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Authors: Tegene Nigussie

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Introduction: Maize is the most domesticated of all the field crops. Wild maize has not been found to date and there has been much speculation on its origin. Regardless of the validity of different theories, it is generally agreed that the center of origin of maize is Central America, primarily Mexico and the Caribbean. Maize in Africa is of a recent introduction although data suggest that it was present in Nigeria even before Columbus voyages. After being taken to Europe in 1493, maize was introduced to Africa and distributed (spread through the continent by different routes. Maize is an important cereal crop in Ethiopia in general, it is the primarily stable food, and rural households show strong preference. For human food, the important constituents of grain are carbohydrates (starch and sugars), protein, fat or oil (in the embryo) and minerals. About 75 percent of the kernel is starch, a range of 60.80 percent but low protein content (8-15%). In Ethiopia, the introduction of modern farming techniques appears to be a priority. However, the adoption of modern inputs by peasant farmers is found to be very slow, for example, the adoption rate of fertilizer, an input that is relatively adopted, is very slow. The difference in socio-economic factors lay behind the low rate of technological adoption, including price & marketing input. Objective: The aim of the study is to determine the optimum application rate or level of different phosphorus fertilizers for the vegetative growth of maize and to identify the effect of different phosphorus rates on the growth and development of maize. Methods: The vegetative parameter (above ground) measurement from five plants randomly sampled from the middle rows of each plot. Results: The interaction of nitrogen and maize variety showed a significant at (p<0.01) effect on plant height, with the application of 60kg/ha and BH140 maize variety in combination and root length with the application of 60kg/ha of nitrogen and BH140 variety of maize. The highest mean (12.33) of the number of leaves per plant and mean (7.1) of the number of nodes per plant can be used as an alternative for better vegetative growth of maize. Conclusion and Recommendation: Maize is one of the popular and cultivated crops in Ethiopia. This study was conducted to investigate the best dosage of phosphorus for vegetative growth, yield, and better quality of maize variety and to recommend a level of phosphorus rate and the best variety adaptable to the specific soil condition or area.

Keywords: leaf, carbohydrate protein, adoption, sugar

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Authors: Iliyasu A. A. Ibrahim

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Nutrition knowledge, attitudes, and practices (KAPs) play a vital role in preventing malnutrition and its consequences, impacting quality of life. This study aimed to assess KAPs among elderly individuals (60-90 years) in 4 villages of Duguri District, Alkaleri, Nigeria. A cross-sectional study was conducted among 2000 geriatrics from four villages. Studies showed that 70.6% of participants demonstrated poor nutrition knowledge, 60.2% exhibited unhealthy practices, while 50.5% displayed negative attitudes. Village-wise Comparison indicated that Yashi village recorded the lowest poor knowledge score (47.2%), Mainamaji (57.4%), Kungibar (66.2%), and Badara (67.2%) followed. Yashi village showed the most positive attitude (51.1%). The study revealed factors influencing KAPs, such as travel exposure and higher education, correlated with better attitudes and practices. The study highlights the significance of addressing nutrition-related KAP gaps among Duguri district’s elderly. Raising awareness and implementing a nutrition strategy with a focus on older adults is crucial. Concrete measures must ensure elders' nutritional needs are met, enhancing their quality of life.

Keywords: nutrition, knowledge, attitude, practice, elderly, Duguri

Procedia PDF Downloads 19

Authors: Farhana Arefeen Mila, Luis Emilio Morales, Nam Hoang, Sujana Adapa, Garry Griffith

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This study investigates the existence of price transmission asymmetries and imperfections among the coastal and freshwater fish species in Bangladesh. Using a Structural Vector Autoregression (SVAR) model, we explore how price changes in one fish species impact the prices of others in the retail market. Monthly data from the Department of Agricultural Marketing (DAM) covering the period from 2012 to 2023 was analyzed. Price series were detrended using the Hodrick-Prescott filter, and unit root tests confirmed stationarity after detrending. The findings indicate that there are significant interdependencies and asymmetries in price transmission, particularly the strong influence of Hilsha on the broader fish market. Hilsha’s price shocks generate immediate responses across other species, reflecting its cultural and economic importance. Silver Pomfret demonstrates some independence but is still affected by broader market fluctuations, particularly those involving Hilsha. Meanwhile, Rohu and Catla exhibit high interdependence, where price changes in one species closely impact the other, underscoring their substitutable nature in consumer preferences. These findings emphasize the need for joint interventions and market monitoring to stabilize prices effectively. Stakeholders are encouraged to monitor Hilsha’s market, consider coordinated interventions for Rohu and Catla, and establish data-sharing partnerships to enhance market stability. Additionally, promoting consumer awareness of price trends and sustainable practices can further support market resilience and long-term sustainability in the fisheries sector.

Keywords: price transmission, cross commodity, fish, Bangladesh, CCF, SVAR, IRF

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