Search results for: Relational Database

Authors: Sana Ktata, Kaïs Ouni, Noureddine Ellouze

Abstract:

Electrocardiogram (ECG) data compression algorithm is needed that will reduce the amount of data to be transmitted, stored and analyzed, but without losing the clinical information content. A wavelet ECG data codec based on the Set Partitioning In Hierarchical Trees (SPIHT) compression algorithm is proposed in this paper. The SPIHT algorithm has achieved notable success in still image coding. We modified the algorithm for the one-dimensional (1-D) case and applied it to compression of ECG data. By this compression method, small percent root mean square difference (PRD) and high compression ratio with low implementation complexity are achieved. Experiments on selected records from the MIT-BIH arrhythmia database revealed that the proposed codec is significantly more efficient in compression and in computation than previously proposed ECG compression schemes. Compression ratios of up to 48:1 for ECG signals lead to acceptable results for visual inspection.

Keywords: Discrete Wavelet Transform, ECG compression, SPIHT.

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Authors: Ali Cheraghian, Farshid Hajati, Soheila Gheisari, Yongsheng Gao

Abstract:

In this paper, we present a novel 2.5D face recognition method based on Gabor Discrete Cosine Transform (GDCT). In the proposed method, the Gabor filter is applied to extract feature vectors from the texture and the depth information. Then, Discrete Cosine Transform (DCT) is used for dimensionality and redundancy reduction to improve computational efficiency. The system is combined texture and depth information in the decision level, which presents higher performance compared to methods, which use texture and depth information, separately. The proposed algorithm is examined on publically available Bosphorus database including models with pose variation. The experimental results show that the proposed method has a higher performance compared to the benchmark.

Keywords: Gabor filter, discrete cosine transform, 2.5D face recognition, pose.

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Authors: Carlos Roberto Valˆencio, F´abio Renato de Almeida, Thatiane Kawabata, Leandro Alves Neves, Julio Cesar Momente, Mario Luiz Tronco, Angelo Cesar Colombini

Abstract:

By running transactions under the SNAPSHOT isolation we can achieve a good level of concurrency, specially in databases with high-intensive read workloads. However, SNAPSHOT is not immune to all the problems that arise from competing transactions and therefore no serialization warranty exists. We propose in this paper a technique to obtain data consistency with SNAPSHOT by using some special triggers that we named DAEMON TRIGGERS. Besides keeping the benefits of the SNAPSHOT isolation, the technique is specially useful for those database systems that do not have an isolation level that ensures serializability, like Firebird and Oracle. We describe all the anomalies that might arise when using the SNAPSHOT isolation and show how to preclude them with DAEMON TRIGGERS. Based on the methodology presented here, it is also proposed the creation of a new isolation level: DAEMON SNAPSHOT.

Keywords: Data consistency, serialization, snapshot.

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Authors: Daomao Yang, Qizhi Wang

Abstract:

Monoterpenoids are mainly found in plant essential oils and they are ideal substrates for biotransformation into oxygen-containing derivatives with important commercial value due to their low price and simple structure. In this paper, eleven strains of endophytic fungi from Praxelis clematidea were used as test strains to conduct the whole cell biotransformation of the monoterpenoids: (+)-limonene, (-)-limonene and myrcene. The fungi were inoculated in 50 ml Sabouraud medium and incubated at 30 ℃ with the agitation of 150 r/min for 6 d, and then 0.5% (v/v) substrates were added into the medium and biotransformed for further 3 d. Afterwards the cultures were filtered, and extracted using equal volume of ethyl acetate. The metabolites were analyzed by GC-MS technique with NIST database. The Total Ion Chromatogram of the extractions from the eleven strains showed that the main product of (+)- and (-)-limonene biotransformation was limonene-1,2-diol, while it is limonene and linalool oxide for biotransformation of myrcene. This work will help screen the microorganisms to biotransform the monoterpenes.

Keywords: Endophytic fungi, (+)–limonene, (-)–limonene, myrcene.

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Authors: Yu Bin, Zhang Yan

Abstract:

The prediction of transmembrane helical segments (TMHs) in membrane proteins is an important field in the bioinformatics research. In this paper, a new method based on discrete wavelet transform (DWT) has been developed to predict the number and location of TMHs in membrane proteins. PDB coded as 1KQG was chosen as an example to describe the prediction of the number and location of TMHs in membrane proteins by using this method. To access the effect of the method, 80 proteins with known 3D-structure from Mptopo database are chosen at random as the test objects (including 325 TMHs), 308 of which can be predicted accurately, the average predicted accuracy is 96.3%. In addition, the above 80 membrane proteins are divided into 13 groups according to their function and type. In particular, the results of the prediction of TMHs of the 13 groups are satisfying.

Keywords: discrete wavelet transform, hydrophobicity, membrane protein, transmembrane helical segments

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Authors: Morteza Moazami-Goudarzi, Ali Taheri, Mohammad Pooyan, Reza Mahboobi

Abstract:

In this paper we are to find the optimum multiwavelet for compression of electrocardiogram (ECG) signals and then, selecting it for using with SPIHT codec. At present, it is not well known which multiwavelet is the best choice for optimum compression of ECG. In this work, we examine different multiwavelets on 24 sets of ECG data with entirely different characteristics, selected from MIT-BIH database. For assessing the functionality of the different multiwavelets in compressing ECG signals, in addition to known factors such as Compression Ratio (CR), Percent Root Difference (PRD), Distortion (D), Root Mean Square Error (RMSE) in compression literature, we also employed the Cross Correlation (CC) criterion for studying the morphological relations between the reconstructed and the original ECG signal and Signal to reconstruction Noise Ratio (SNR). The simulation results show that the Cardinal Balanced Multiwavelet (cardbal2) by the means of identity (Id) prefiltering method to be the best effective transformation. After finding the most efficient multiwavelet, we apply SPIHT coding algorithm on the transformed signal by this multiwavelet.

Keywords: ECG compression, Prefiltering, Cardinal Balanced Multiwavelet.

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Authors: Randa I. M. Elanwar, Mohsen A. Rashwan, Samia A. Mashali

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In this paper we present the first Arabic sentence dataset for on-line handwriting recognition written on tablet pc. The dataset is natural, simple and clear. Texts are sampled from daily newspapers. To collect naturally written handwriting, forms are dictated to writers. The current version of our dataset includes 154 paragraphs written by 48 writers. It contains more than 3800 words and more than 19,400 characters. Handwritten texts are mainly written by researchers from different research centers. In order to use this dataset in a recognition system word extraction is needed. In this paper a new word extraction technique based on the Arabic handwriting cursive nature is also presented. The technique is applied to this dataset and good results are obtained. The results can be considered as a bench mark for future research to be compared with.

Keywords: Arabic, Handwriting recognition, on-line dataset.

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Authors: J. S. Nah, A. Y. Jeon, J. H. Ro, G. R. Jeon

Abstract:

ECG analysis method was developed using ROC analysis of PVC detecting algorithm. ECG signal of MIT-BIH arrhythmia database was analyzed by MATLAB. First of all, the baseline was removed by median filter to preprocess the ECG signal. R peaks were detected for ECG analysis method, and normal VCG was extracted for VCG analysis method. Four PVC detecting algorithm was analyzed by ROC curve, which parameters are maximum amplitude of QRS complex, width of QRS complex, r-r interval and geometric mean of VCG. To set cut-off value of parameters, ROC curve was estimated by true-positive rate (sensitivity) and false-positive rate. sensitivity and false negative rate (specificity) of ROC curve calculated, and ECG was analyzed using cut-off value which was estimated from ROC curve. As a result, PVC detecting algorithm of VCG geometric mean have high availability, and PVC could be detected more accurately with amplitude and width of QRS complex.

Keywords: Vectorcardiogram (VCG), Premature Ventricular contraction (PVC), ROC (receiver operating characteristic) curve, ECG

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Authors: Winai Mankhatitham

Abstract:

This research investigated groundcover on the roof (green roof) which can reduce the temperature and carbon monoxide. This study is divided into 3 main aspects: 1. Types of groundcover affecting heat reduction 2. The efficiency on heat reduction of 3 types of groundcover, i.e. lawn, arachis pintoi, and purslane 3. Database for designing green roof. This study has been designed as an experimental research by simulating the 3 types of groundcover in 3 trays placed in the green house for recording the temperature change for 24 hours. The results showed that the groundcover with the highest heat reduction efficiency was lawn. The dense of the lawn can protect the heat transfer to the soil. For the further study, there should be a comparative study of the thickness and the types of soil to get more information for the suitable types of groundcover and the soil for designing the energy saving green roof.

Keywords: Groundcover, Green Roof, Heat Reduction, Energy Saving.

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Authors: Jaemoon Lim

Abstract:

To construct the lumped spring-mass model considering the occupants for the offset frontal crash, the SISAME software and the NHTSA test data were used. The data on 56 kph 40% offset frontal vehicle to deformable barrier crash test of a MY2007 Mazda 6 4-door sedan were obtained from NHTSA test database. The overall behaviors of B-pillar and engine of simulation models agreed very well with the test data. The trends of accelerations at the driver and passenger head were similar but big differences in peak values. The differences of peak values caused the large errors of the HIC36 and 3 ms chest g’s. To predict well the behaviors of dummies, the spring-mass model for the offset frontal crash needs to be improved.

Keywords: Chest g’s, HIC36, lumped spring-mass model, offset frontal impact, SISAME.

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Authors: Lae-Jeong Park, Kyusoo Chung, Jungho Moon

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This paper introduces an effective method of segmenting Korean text (place names in Korean) from a Korean road sign image. A Korean advanced directional road sign is composed of several types of visual information such as arrows, place names in Korean and English, and route numbers. Automatic classification of the visual information and extraction of Korean place names from the road sign images make it possible to avoid a lot of manual inputs to a database system for management of road signs nationwide. We propose a series of problem-specific heuristics that correctly segments Korean place names, which is the most crucial information, from the other information by leaving out non-text information effectively. The experimental results with a dataset of 368 road sign images show 96% of the detection rate per Korean place name and 84% per road sign image.

Keywords: Segmentation, road signs, characters, classification.

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Authors: Hazem M. El-Bakry, Ahmed A. Saleh, Taghreed T. Asfour

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Adaptive e-learning today gives the student a central role in his own learning process. It allows learners to try things out, participate in courses like never before, and get more out of learning than before. In this paper, an adaptive e-learning model for logic design, simplification of Boolean functions and related fields is presented. Such model presents suitable courses for each student in a dynamic and adaptive manner using existing database and workflow technologies. The main objective of this research work is to provide an adaptive e-learning model based learners' personality using explicit and implicit feedback. To recognize the learner-s, we develop dimensions to decide each individual learning style in order to accommodate different abilities of the users and to develop vital skills. Thus, the proposed model becomes more powerful, user friendly and easy to use and interpret. Finally, it suggests a learning strategy and appropriate electronic media that match the learner-s preference.

Keywords: Adaptive learning, Learning styles, Teaching strategies.

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Authors: T. S. Chou, K. K. Yen, J. Luo

Abstract:

The network traffic data provided for the design of intrusion detection always are large with ineffective information and enclose limited and ambiguous information about users- activities. We study the problems and propose a two phases approach in our intrusion detection design. In the first phase, we develop a correlation-based feature selection algorithm to remove the worthless information from the original high dimensional database. Next, we design an intrusion detection method to solve the problems of uncertainty caused by limited and ambiguous information. In the experiments, we choose six UCI databases and DARPA KDD99 intrusion detection data set as our evaluation tools. Empirical studies indicate that our feature selection algorithm is capable of reducing the size of data set. Our intrusion detection method achieves a better performance than those of participating intrusion detectors.

Keywords: Intrusion detection, feature selection, k-nearest neighbors, fuzzy clustering, Dempster-Shafer theory

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Authors: Yoshifumi Ishizaki, Naoki Kanatani, Masaki Ito, Toshihiko Sasama, Takao Kawamura, Kazunori Sugahara

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This paper demonstrates the bus location system for the route bus through the experiment in the real environment. A bus location system is a system that provides information such as the bus delay and positions. This system uses actual services and positions data of buses, and those information should match data on the database. The system has two possible problems. One, the system could cost high in preparing devices to get bus positions. Two, it could be difficult to match services data of buses. To avoid these problems, we have developed this system at low cost and short time by using the smart phone with GPS and the bus route system. This system realizes the path planning considering bus delay and displaying position of buses on the map. The bus location system was demonstrated on route buses with smart phones for two months.

Keywords: Route Bus, Path Planning System, GPS, Smart Phone.

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Authors: Nermine Ahmed Hendy, Hania Farag

Abstract:

Emotion recognition is an important research field that finds lots of applications nowadays. This work emphasizes on recognizing different emotions from speech signal. The extracted features are related to statistics of pitch, formants, and energy contours, as well as spectral, perceptual and temporal features, jitter, and shimmer. The Artificial Neural Networks (ANN) was chosen as the classifier. Working on finding a robust and fast ANN classifier suitable for different real life application is our concern. Several experiments were carried out on different ANN to investigate the different factors that impact the classification success rate. Using a database containing 7 different emotions, it will be shown that with a proper and careful adjustment of features format, training data sorting, number of features selected and even the ANN type and architecture used, a success rate of 85% or even more can be achieved without increasing the system complicity and the computation time

Keywords: Classification, emotion recognition, features extraction, feature selection, neural network

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Authors: Yan Ma, ShunBao Li

Abstract:

A new approach is adopted in this paper based on Turk and Pentland-s eigenface method. It was found that the probability density function of the distance between the projection vector of the input face image and the average projection vector of the subject in the face database, follows Rayleigh distribution. In order to decrease the false acceptance rate and increase the recognition rate, the input face image has been recognized using two thresholds including the acceptance threshold and the rejection threshold. We also find out that the value of two thresholds will be close to each other as number of trials increases. During the training, in order to reduce the number of trials, the projection vectors for each subject has been averaged. The recognition experiments using the proposed algorithm show that the recognition rate achieves to 92.875% whilst the average number of judgment is only 2.56 times.

Keywords: Eigenface, Face Recognition, Threshold, Rayleigh Distribution, Feature Extraction

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Authors: Chien-Hua Wang, Wei-Hsuan Lee, Chin-Tzong Pang

Abstract:

In data mining, the association rules are used to find for the associations between the different items of the transactions database. As the data collected and stored, rules of value can be found through association rules, which can be applied to help managers execute marketing strategies and establish sound market frameworks. This paper aims to use Fuzzy Frequent Pattern growth (FFP-growth) to derive from fuzzy association rules. At first, we apply fuzzy partition methods and decide a membership function of quantitative value for each transaction item. Next, we implement FFP-growth to deal with the process of data mining. In addition, in order to understand the impact of Apriori algorithm and FFP-growth algorithm on the execution time and the number of generated association rules, the experiment will be performed by using different sizes of databases and thresholds. Lastly, the experiment results show FFPgrowth algorithm is more efficient than other existing methods.

Keywords: Data mining, association rule, fuzzy frequent patterngrowth.

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Authors: Baharak Khoshkholgh-Sima, Soroush Sardari, Jalal Izadi Mobarakeh, Ramezan Ali Khavari-Nejad

Abstract:

There is an urgent need to develop novel Mycobacterium tuberculosis (Mtb) drugs that are active against drug resistant bacteria but, more importantly, kill persistent bacteria. Our study structured based on integrated analysis of metabolic pathways, small molecule screening and similarity Search in PubChem Database. Metabolic analysis approaches based on Unified weighted used for potent target selection. Our results suggest that pantothenate synthetase (panC) and and 3-methyl-2-oxobutanoate hydroxymethyl transferase (panB) as a appropriate drug targets. In our study, we used pantothenate synthetase because of existence inhibitors. We have reported the discovery of new antitubercular compounds through ligand based approaches using computational tools.

Keywords: In Silico, Ligand-based Virtual Screening, Metabolic Pathways, Mycobacterium tuberculosis

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Authors: Liang Zhao, Guangwen Wang, Guichang Liu

Abstract:

Constant potometer is an important component of pipeline anti-corrosion systems in the chemical industry. Based on Internet of Things (IoT) technology, Programmable Logic Controller (PLC) technology and database technology, this paper developed a set of a constant potometer remote monitoring management system. The remote monitoring and remote adjustment of the working status of the constant potometer are realized. The system has real-time data display, historical data query, alarm push management, user permission management, and supporting Web access and mobile client application (APP) access. The actual engineering project test results show the stability of the system, which can be widely used in cathodic protection systems.

Keywords: Internet of Things, pipe corrosion protection, potentiostat, remote monitoring.

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Authors: Suguru Yoshioka, Seiichi Tani, Seinosuke Toda

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In historical science and social science, the influence of natural disaster upon society is a matter of great interest. In recent years, some archives are made through many hands for natural disasters, however it is inefficiency and waste. So, we suppose a computer system to create a historical natural disaster archive. As the target of this analysis, we consider newspaper articles. The news articles are considered to be typical examples that prescribe the temporal relations of affairs for natural disaster. In order to do this analysis, we identify the occurrences in newspaper articles by some index entries, considering the affairs which are specific to natural disasters, and show the temporal relation between natural disasters. We designed and implemented the automatic system of “extraction of the occurrences of natural disaster" and “temporal relation table for natural disaster."

Keywords: Database, digital library, corpus, historical natural disaster, temporal relation

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Authors: Viet Dzung Nguyen, Duc Thuan Nguyen, Tien Dzung Nguyen, Van Thanh Pham

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Mammography is the most effective procedure for an early diagnosis of the breast cancer. Nowadays, people are trying to find a way or method to support as much as possible to the radiologists in diagnosis process. The most popular way is now being developed is using Computer-Aided Detection (CAD) system to process the digital mammograms and prompt the suspicious region to radiologist. In this paper, an automated CAD system for detection and classification of massive lesions in mammographic images is presented. The system consists of three processing steps: Regions-Of- Interest detection, feature extraction and classification. Our CAD system was evaluated on Mini-MIAS database consisting 322 digitalized mammograms. The CAD system-s performance is evaluated using Receiver Operating Characteristics (ROC) and Freeresponse ROC (FROC) curves. The archived results are 3.47 false positives per image (FPpI) and sensitivity of 85%.

Keywords: classification, computer-aided detection, featureextraction, mass detection.

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Authors: M. Hassan, I. Osman, M. Yahia

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This Paper proposes a new facial feature extraction approach, Wash-Hadamard Transform (WHT). This approach is based on correlation between local pixels of the face image. Its primary advantage is the simplicity of its computation. The paper compares the proposed approach, WHT, which was traditionally used in data compression with two other known approaches: the Principal Component Analysis (PCA) and the Discrete Cosine Transform (DCT) using the face database of Olivetti Research Laboratory (ORL). In spite of its simple computation, the proposed algorithm (WHT) gave very close results to those obtained by the PCA and DCT. This paper initiates the research into WHT and the family of frequency transforms and examines their suitability for feature extraction in face recognition applications.

Keywords: Face Recognition, Facial Feature Extraction, Principal Component Analysis, and Discrete Cosine Transform, Wash-Hadamard Transform.

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Authors: Y. H. Tsai, Y. W. Chen

Abstract:

Facial features are frequently used to represent local properties of a human face image in computer vision applications. In this paper, we present a fast algorithm that can extract the facial features online such that they can give a satisfying representation of a face image. It includes one step for a coarse detection of each facial feature by AdaBoost and another one to increase the accuracy of the found points by Active Shape Models (ASM) in the regions of interest. The resulted facial features are evaluated by matching with artificial face models in the applications of physiognomy. The distance measure between the features and those in the fate models from the database is carried out by means of the Hausdorff distance. In the experiment, the proposed method shows the efficient performance in facial feature extractions and online system of physiognomy.

Keywords: Facial feature extraction, AdaBoost, Active shapemodel, Hausdorff distance

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Authors: Abder-Rahman Ali, Adélaïde Albouy-Kissi, Manuel Grand-Brochier, Viviane Ladan-Marcus, Christine Hoeffl, Claude Marcus, Antoine Vacavant, Jean-Yves Boire

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In this paper, we present a new segmentation approach for focal liver lesions in contrast enhanced ultrasound imaging. This approach, based on a two-cluster Fuzzy C-Means methodology, considers type-II fuzzy sets to handle uncertainty due to the image modality (presence of speckle noise, low contrast, etc.), and to calculate the optimum inter-cluster threshold. Fine boundaries are detected by a local recursive merging of ambiguous pixels. The method has been tested on a representative database. Compared to both Otsu and type-I Fuzzy C-Means techniques, the proposed method significantly reduces the segmentation errors.

Keywords: Defuzzification, fuzzy clustering, image segmentation, type-II fuzzy sets.

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Authors: Khalid A. Kaabneh

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This paper introduces and studies new indexing techniques for content-based queries in images databases. Indexing is the key to providing sophisticated, accurate and fast searches for queries in image data. This research describes a new indexing approach, which depends on linear modeling of signals, using bases for modeling. A basis is a set of chosen images, and modeling an image is a least-squares approximation of the image as a linear combination of the basis images. The coefficients of the basis images are taken together to serve as index for that image. The paper describes the implementation of the indexing scheme, and presents the findings of our extensive evaluation that was conducted to optimize (1) the choice of the basis matrix (B), and (2) the size of the index A (N). Furthermore, we compare the performance of our indexing scheme with other schemes. Our results show that our scheme has significantly higher performance.

Keywords: Axial Projection, images, indexing, multimedia database, searching.

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Authors: Yi-an Cui, Lu Wang, Jian-ping Xiao

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This paper presents an automatic feature recognition method based on center-surround difference detecting and fuzzy logic that can be applied in ground-penetrating radar (GPR) image processing. Adopted center-surround difference method, the salient local image regions are extracted from the GPR images as features of detected objects. And fuzzy logic strategy is used to match the detected features and features in template database. This way, the problem of objects detecting, which is the key problem in GPR image processing, can be converted into two steps, feature extracting and matching. The contributions of these skills make the system have the ability to deal with changes in scale, antenna and noises. The results of experiments also prove that the system has higher ratio of features sensing in using GPR to image the subsurface structures.

Keywords: feature recognition, GPR image, matching strategy, salient image

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Authors: Indradip Banerjee, Souvik Bhattacharyya, Gautam Sanyal

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‘Steganalysis’ is one of the challenging and attractive interests for the researchers with the development of information hiding techniques. It is the procedure to detect the hidden information from the stego created by known steganographic algorithm. In this paper, a novel feature based image steganalysis technique is proposed. Various statistical moments have been used along with some similarity metric. The proposed steganalysis technique has been designed based on transformation in four wavelet domains, which include Haar, Daubechies, Symlets and Biorthogonal. Each domain is being subjected to various classifiers, namely K-nearest-neighbor, K* Classifier, Locally weighted learning, Naive Bayes classifier, Neural networks, Decision trees and Support vector machines. The experiments are performed on a large set of pictures which are available freely in image database. The system also predicts the different message length definitions.

Keywords: Steganalysis, Moments, Wavelet Domain, KNN, K*, LWL, Naive Bayes Classifier, Neural networks, Decision trees, SVM.

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Authors: C. Senanayake, S. Veera Ragavan

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Warehousing is commonly used in factories for the storage of products until delivery of orders. As the amount of products stored increases it becomes tedious to be carried out manually. In recent years, the manual storing has converted into fully or partially computer controlled systems, also known as Automated Storage and Retrieval Systems (AS/RS). This paper discusses an ASRS system, which was designed such that the best storage location for the products is determined by utilizing a fuzzy control system. The design maintains the records of the products to be/already in store and the storage/retrieval times along with the availability status of the storage locations. This paper discusses on the maintenance of the above mentioned records and the utilization of the concept of fuzzy logic in order to determine the optimum storage location for the products. The paper will further discuss on the dynamic splitting and merging of the storage locations depending on the product sizes.

Keywords: ASRS, fuzzy control systems, MySQL database, dynamic splitting and merging.

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Authors: Zhizhong Jiang, Peter Naudé

Abstract:

Effective evaluation of software development effort is an important aspect of successful project management. Based on a large database with 4106 projects ever developed, this study statistically examines the factors that influence development effort. The factors found to be significant for effort are project size, average number of developers that worked on the project, type of development, development language, development platform, and the use of rapid application development. Among these factors, project size is the most critical cost driver. Unsurprisingly, this study found that the use of CASE tools does not necessarily reduce development effort, which adds support to the claim that the use of tools is subtle. As many of the current estimation models are rarely or unsuccessfully used, this study proposes a parsimonious parametric model for the prediction of effort which is both simple and more accurate than previous models.

Keywords: Development effort, function points, team size, development language, CASE tool, rapid application development.

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Authors: Dalanya Asaad Mohammed, Dara Abdul Razaq

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A total of twenty tensile biopsies were collected from children undergoing tonsillectomy from teaching hospital ENT department and Kurdistan private hospital in sulaimani city. All biopsies were homogenized and cultured; the obtained bacterial isolates were purified and identified by biochemical tests and VITEK 2 compact system. Among the twenty studied samples, only one Pseudomonas putida with probability of 99% was isolated. Antimicrobial susceptibility was carried out by disk diffusion method, Pseudomonas putida showed resistance to all antibiotics used except vancomycin. The isolate further subjected to PCR and DNA sequence analysis of blaVIM gene using different set of primers for different regions of VIM gene. The results were found to be PCR positive for the blaVIM gene. To determine the sequence of blaVIM gene, DNA sequencing performed. Sequence alignment of blaVIM gene with previously recorded blaVIM gene in NCBI- database showed that P. putida isolate have different blaVIM gene.

Keywords: Clinical isolates, Putida, Sulaimani, Vim gene.

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