Search results for: Ping Huang

Authors: Steven W. Su , Hung Nguyen, Rob Jarman, Joe Zhu, David Lowe, Peter McLean, Shoudong Huang, Nghia T. Nguyen, Russell Nicholson, Kaili Weng

Abstract:

This paper proposed a nonlinear model predictive control (MPC) method for the control of gantry crane. One of the main motivations to apply MPC to control gantry crane is based on its ability to handle control constraints for multivariable systems. A pre-compensator is constructed to compensate the input nonlinearity (nonsymmetric dead zone with saturation) by using its inverse function. By well tuning the weighting function matrices, the control system can properly compromise the control between crane position and swing angle. The proposed control algorithm was implemented for the control of gantry crane system in System Control Lab of University of Technology, Sydney (UTS), and achieved desired experimental results.

Keywords: Model Predictive Control, Control constraints, Input nonlinearity compensation, Overhead gantry crane.

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Authors: Y. Li, Q. S. Zhang, H. Z. Huang, B. Y. Wu

Abstract:

The mechanism behind the electromigration and thermomigration failure in flip-chip solder joints with Cu-pillar bumps was investigated in this paper through using finite element method. Hot spot and the current crowding occurrs in the upper corner of copper column instead of solders of the common solder ball. The simulation results show that the change in thermal gradient is noticeable, which might greatly affect the reliability of solder joints with Cu-pillar bumps under current stressing. When the average applied current density is increased from 1×104 A/cm2 to 3×104 A/cm2 in solders, the thermal gradient would increase from 74 K/cm to 901 K/cm at an ambient temperature of 25°C. The force from thermal gradient of 901 K/cm can nearly induce thermomigration by itself. With the increase in applied current, the thermal gradient is growing. It is proposed that thermomigration likely causes a serious reliability issue for Cu column based interconnects.

Keywords: Simulation, Cu-pillar bumps, Electromigration, Thermomigration.

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Authors: Meng-Hao Tsai, Tsuei-Chiang Huang

Abstract:

Interior brick-infill partitions are usually considered as non-structural components and only their weight is accounted for in practical structural design. In this study, their effect on the progressive collapse resistance of an RC building subjected to sudden column loss is investigated. Three notional column loss conditions with four different brick-infill locations are considered. Column-loss response analyses of the RC building with and without brick infills are carried out. Analysis results indicate that the collapse resistance is only slightly influenced by the brick infills due to their brittle failure characteristic. Even so, they may help to reduce the inelastic displacement response under column loss. For practical engineering, it is reasonably conservative to only consider the weight of brick-infill partitions in the structural analysis.

Keywords: Progressive collapse, column loss, brick-infill partition, compression strut.

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Authors: Wei-Hwa Chiang, Hao-Hsiang Hsu, Jian-Sheng Huang

Abstract:

Appropriate ventilation in a classroom is helpful for enhancing air exchange rate and student concentration. This study focuses on the effects of fenestration in a four-story school building by performing numerical simulation of a building when considering indoor and outdoor environments simultaneously. The wind profile function embedded in PHOENICS code was set as the inlet boundary condition in a suburban environment. Sixteen fenestration combinations were compared in a classroom containing thirty seats. This study evaluates mean age of air (AGE) and airflow pattern of a classroom on different floors. Considering both wind profile and fenestration effects, the airflow on higher floors is channeled toward the area near ceiling in a room and causes older mean age of air in the breathing zone. The results in this study serve as a useful guide for enhancing natural ventilation in a typical school building.

Keywords: Cross ventilation, Fenestration effect, Wind profile, Mean age of air, CFD

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Authors: Ruey-Yu Chen, Yao-Tsung Chang, Ching-Ying Yeh, Yu-Ting Huang

Abstract:

Musculoskeletal problems in the hotel sector have been little studied. The aim of this study was to examine relationships of musculoskeletal pain and work characteristics with presenteeism, i.e., feeling sick but going to work anyway. Data of a self-reported questionnaire were collected from 1,101 employees, who joined the study on a voluntary basis from four hotels in northern Taiwan. The results showed that respondents who were female, were younger, had a higher educational level, and worked in the real-service department had higher presenteeism. There were significant positive associations between presenteeism and heavy loads, frequent beatings or hits of hard objects, improper bench height, employees’ lower limb and lower back pain. Our study results imply that knowledge of work characteristics and employees' musculoskeletal problems could be advantageously used to reduce presenteeism in the workplace.

Keywords: Musculoskeletal pain, absenteeism, presenteeism, hotel employees.

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Authors: Yu-Shuai Chen , Chien-Ching Chiu , Chung-Hsin Huang, Chien-Hung Chen

Abstract:

The bit error rate (BER) performance for ultra-wide band (UWB) indoor communication with impact of metallic furniture is investigated. The impulse responses of different indoor environments for any transmitter and receiver location are computed by shooting and bouncing ray/image and inverse Fourier transform techniques. By using the impulse responses of these multipath channels, the BER performance for binary pulse amplitude modulation (BPAM) impulse radio UWB communication system are calculated. Numerical results have shown that the multi-path effect by the metallic cabinets is an important factor for BER performance. Also the outage probability for the UWB multipath environment with metallic cabinets is more serious (about 18%) than with wooden cabinets. Finally, it is worth noting that in these cases the present work provides not only comparative information but also quantitative information on the performance reduction.

Keywords: UWB, multipath, outage probability.

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Authors: Cheng-Lung Huang, Han-Yu Chien, Michael Conyette

Abstract:

Social bookmarking is an environment in which the user gradually changes interests over time so that the tag data associated with the current temporal period is usually more important than tag data temporally far from the current period. This implies that in the social tagging system, the newly tagged items by the user are more relevant than older items. This study proposes a novel recommender system that considers the users- recent tag preferences. The proposed system includes the following stages: grouping similar users into clusters using an E-M clustering algorithm, finding similar resources based on the user-s bookmarks, and recommending the top-N items to the target user. The study examines the system-s information retrieval performance using a dataset from del.icio.us, which is a famous social bookmarking web site. Experimental results show that the proposed system is better and more effective than traditional approaches.

Keywords: Recommender systems, Social bookmarking, Tag

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Authors: Xinde Li, Xinhan Huang, Min Wang

Abstract:

In this paper, we propose the pre-processor based on the Evidence Supporting Measure of Similarity (ESMS) filter and also propose the unified fusion approach (UFA) based on the general fusion machine coupled with ESMS filter, which improve the correctness and precision of information fusion in any fields of application. Here we mainly apply the new approach to Simultaneous Localization And Mapping (SLAM) of Pioneer II mobile robots. A simulation experiment was performed, where an autonomous virtual mobile robot with sonar sensors evolves in a virtual world map with obstacles. By comparing the result of building map according to the general fusion machine (here DSmT-based fusing machine and PCR5-based conflict redistributor considereded) coupling with ESMS filter and without ESMS filter, it shows the benefit of the selection of the sources as a prerequisite for improvement of the information fusion, and also testifies the superiority of the UFA in dealing with SLAM.

Keywords: DSmT, ESMS filter, SLAM, UFA

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Authors: Wen-Lung Huang, Shih-Pin Chen

Abstract:

This paper investigates the optimization problem of multi-product aggregate production planning (APP) with fuzzy data. From a comprehensive viewpoint of conserving the fuzziness of input information, this paper proposes a method that can completely describe the membership function of the performance measure. The idea is based on the well-known Zadeh-s extension principle which plays an important role in fuzzy theory. In the proposed solution procedure, a pair of mathematical programs parameterized by possibility level a is formulated to calculate the bounds of the optimal performance measure at a . Then the membership function of the optimal performance measure is constructed by enumerating different values of a . Solutions obtained from the proposed method contain more information, and can offer more chance to achieve the feasible disaggregate plan. This is helpful to the decision-maker in practical applications.

Keywords: fuzzy data, aggregate production planning, membership function, parametric programming

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Authors: C. C. Huang, M. S. Amirrudin, N. A. Ahamad Noruddin, R. Othman

Abstract:

Anti-money laundering is commonly recognized as a set of procedures, laws or regulations designed to reduce the practice of generating income through illegal actions. In Malaysia, the government and law enforcement agencies have stepped up their capacities and efforts to curb money laundering since 2001. One of these measures was the enactment of the Anti-Money Laundering Act (AMLA) in 2001. The implementation costs on anti-money laundering requirements (AMLR) can be burdensome to those who are involved in enforcing them. The objective of this paper is to explore the perceived effectiveness of AMLR from the enforcement agencies- perspective. This is a preliminary study whose findings will help to give direction for further AML research in Malaysia. In addition, the results of this study provide empirical evidences on the perceived effectiveness of AMLR prior to further investigations on barriers and improvements of the implementation of the anti-money laundering regime in Malaysia.

Keywords: Anti-money laundering, anti-money laundering requirements, perceived effectiveness, enforcement agencies.

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Authors: Zhong-xi Gao, Hou-biao Li

Abstract:

Recently, some convergent results of the generalized AOR iterative (GAOR) method for solving linear systems with strictly diagonally dominant matrices are presented in [Darvishi, M.T., Hessari, P.: On convergence of the generalized AOR method for linear systems with diagonally dominant cofficient matrices. Appl. Math. Comput. 176, 128-133 (2006)] and [Tian, G.X., Huang, T.Z., Cui, S.Y.: Convergence of generalized AOR iterative method for linear systems with strictly diagonally dominant cofficient matrices. J. Comp. Appl. Math. 213, 240-247 (2008)]. In this paper, we give the convergence of the GAOR method for linear systems with strictly doubly diagonally dominant matrix, which improves these corresponding results.

Keywords: Diagonally dominant matrix, GAOR method, Linear system, Convergence

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Authors: Huang Shengqin, Xiao Hong

Abstract:

LES with mixed subgrid-scale model has been used to simulate aerodynamic performance of hypersonic configuration. The simulation was conducted to replicate conditions and geometry of a model which has been previously tested. LES Model has been successful in predict pressure coefficient with the max error 1.5% besides afterbody. But in the high Mach number condition, it is poor in predict ability and product 12.5% error. The calculation error are mainly conducted by the distribution swirling. The fact of poor ability in the high Mach number and afterbody region indicated that the mixed subgrid-scale model should be improved in large eddied especially in hypersonic separate region. In the condition of attach and sideslip flight, the calculation results have waves. LES are successful in the prediction the pressure wave in hypersonic flow.

Keywords: Hypersonic, LES, mixed Subgrid-scale model, experiment.

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Authors: Hsiu-Pi Lin, Wen-Chen Huang, Hui-Fang Chen, Yan-Pin Ke

Abstract:

Corporate Social Responsibility (CSR) has become a new trend of business governance. Few research studies on CSR published in Taiwanese academia, especially for medical settings, we were interested in probing the relationship of CSR and financial performance in medical settings in Taiwan. The results illustrate that: (1) a time delay effect exists with a lag between CSR effort and its performance in the hospital foundation, (2) input into the internal domains of CSR will be helpful to improve employee productivity in the hospital foundation, and (3) input into the external domains of CSR will be helpful in improving financial performance in the hospital foundation. This study overviews CSR in the medical industry in Taiwan and the relationship of CSR and financial performance. Discussions of possible implications from the study results are applied to consult the CSR concept that will be transferred into a business strategy for the organization manager.

Keywords: Corporate Social Responsibility (CSR), financialperformance, hospital foundation,

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Authors: Muhammad R. Ahmed, Xu Huang, Dharmendra Sharma

Abstract:

Despite extensive study on wireless sensor network security, defending internal attacks and finding abnormal behaviour of the sensor are still difficult and unsolved task. The conventional cryptographic technique does not give the robust security or detection process to save the network from internal attacker that cause by abnormal behavior. The insider attacker or abnormally behaved sensor identificationand location detection framework using false massage detection and Time difference of Arrival (TDoA) is presented in this paper. It has been shown that the new framework can efficiently identify and detect the insider attacker location so that the attacker can be reprogrammed or subside from the network to save from internal attack.

Keywords: Insider Attaker identification, Abnormal Behaviour, Location detection, Time difference of Arrival (TDoA), Wireless sensor network

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Authors: Shiuh-Jer Huang, Yu-Sheng Hsu

Abstract:

On-board available parking space detecting system, parking trajectory planning and tracking control mechanism are the key components of vehicle backward auto-parking system. Firstly, pair of ultrasonic sensors is installed on each side of vehicle body surface to detect the relative distance between ego-car and surrounding obstacle. The dimension of a found empty space can be calculated based on vehicle speed and the time history of ultrasonic sensor detecting information. This result can be used for constructing the 2D vehicle environmental map and available parking type judgment. Finally, the auto-parking controller executes the on-line optimal parking trajectory planning based on this 2D environmental map, and monitors the real-time vehicle parking trajectory tracking control. This low cost auto-parking system was tested on a model car.

Keywords: Vehicle auto-parking, parking space detection, parking path tracking, intelligent fuzzy controller.

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Authors: Zhou Yingkun, Xu Guorui, Wei Siming, Huang Yongzhang

Abstract:

With the increasing of the penetration of renewable energy in power system, the whole inertia of the power system is declining, which will endanger the frequency stability of the power system. In order to enhance the inertia, virtual synchronous generator (VSG) has been proposed. In addition, the motor-generator pair (MGP) system is proposed to enhance grid inertia. Both of them need additional equipment to provide instantaneous energy, so the economic problem should be considered. In this paper, the basic working principle of MGP system and VSG are introduced firstly. Then, the technical characteristics and economic investment of MGP/VSG are compared by calculation and simulation. The results show that the MGP system can provide same inertia with less cost than VSG.

Keywords: High renewable energy penetration, inertia of power system, virtual synchronous generator, motor-generator pair system, techno-economic analysis.

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Authors: Shih-Bin Wang, Ping Yuan, Syu-Fang Liu, Ming-Jun Kuo

Abstract:

By the application of an improved back-propagation neural network (BPNN), a model of current densities for a solid oxide fuel cell (SOFC) with 10 layers is established in this study. To build the learning data of BPNN, Taguchi orthogonal array is applied to arrange the conditions of operating parameters, which totally 7 factors act as the inputs of BPNN. Also, the average current densities achieved by numerical method acts as the outputs of BPNN. Comparing with the direct solution, the learning errors for all learning data are smaller than 0.117%, and the predicting errors for 27 forecasting cases are less than 0.231%. The results show that the presented model effectively builds a mathematical algorithm to predict performance of a SOFC stack immediately in real time. Also, the calculating algorithms are applied to proceed with the optimization of the average current density for a SOFC stack. The operating performance window of a SOFC stack is found to be between 41137.11 and 53907.89. Furthermore, an inverse predicting model of operating parameters of a SOFC stack is developed here by the calculating algorithms of the improved BPNN, which is proved to effectively predict operating parameters to achieve a desired performance output of a SOFC stack.

Keywords: a SOFC stack, BPNN, inverse predicting model of operating parameters, optimization of the average current density

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Authors: YaoAn Lu, QingZhen Bi, BaoRui Du, ShuLin Chen, LiMin Zhu, Kai Huang

Abstract:

Taking the design tolerance into account, this paper presents a novel efficient approach to generate iso-scallop tool path for five-axis strip machining with a barrel cutter. The cutter location is first determined on the scallop surface instead of the design surface, and then the cutter is adjusted to locate the optimal tool position based on the differential rotation of the tool axis and satisfies the design tolerance simultaneously. The machining strip width and error are calculated with the aid of the grazing curve of the cutter. Based on the proposed tool positioning algorithm, the tool paths are generated by keeping the scallop height formed by adjacent tool paths constant. An example is conducted to confirm the validity of the proposed method.

Keywords: Strip machining, barrel cutter, iso-scallop tool path, sculptured surfaces, differential motion.

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Authors: Meng-Hui Chen, Pei-Chann Chang, Wei-Hsiu Huang

Abstract:

Artificial Immune System is adopted as a Heuristic Algorithm to solve the combinatorial problems for decades. Nevertheless, many of these applications took advantage of the benefit for applications but seldom proposed approaches for enhancing the efficiency. In this paper, we continue the previous research to develop a Self-evolving Artificial Immune System II via coordinating the T and B cell in Immune System and built a block-based artificial chromosome for speeding up the computation time and better performance for different complexities of problems. Through the design of Plasma cell and clonal selection which are relative the function of the Immune Response. The Immune Response will help the AIS have the global and local searching ability and preventing trapped in local optima. From the experimental result, the significant performance validates the SEAIS II is effective when solving the permutation flows-hop problems.

Keywords: Artificial Immune System, Clonal Selection, Immune Response, Permutation Flow-shop Scheduling Problems

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Authors: Jieh-Haur Chen, Pei-Fen Huang

Abstract:

This paper presents an integrated model that automatically measures the change of rivers, damage area of bridge surroundings, and change of vegetation. The proposed model is on the basis of a neurofuzzy mechanism enhanced by SOM optimization algorithm, and also includes three functions to deal with river imagery. High resolution imagery from FORMOSAT-2 satellite taken before and after the invasion period is adopted. By randomly selecting a bridge out of 129 destroyed bridges, the recognition results show that the average width has increased 66%. The ruined segment of the bridge is located exactly at the most scour region. The vegetation coverage has also reduced to nearly 90% of the original. The results yielded from the proposed model demonstrate a pinpoint accuracy rate at 99.94%. This study brings up a successful tool not only for large-scale damage assessment but for precise measurement to disasters.

Keywords: remote sensing image, damage assessment, typhoon disaster, bridge, ANN, fuzzy, SOM, optimization.

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Authors: Ber-Lin Yu, Ting-Zhu Huang

Abstract:

If there exists a nonempty, proper subset S of the set of all (n+1)(n+2)/2 inertias such that S Ôèå i(A) is sufficient for any n×n zero-nonzero pattern A to be inertially arbitrary, then S is called a critical set of inertias for zero-nonzero patterns of order n. If no proper subset of S is a critical set, then S is called a minimal critical set of inertias. In [Kim, Olesky and Driessche, Critical sets of inertias for matrix patterns, Linear and Multilinear Algebra, 57 (3) (2009) 293-306], identifying all minimal critical sets of inertias for n×n zero-nonzero patterns with n ≥ 3 and the minimum cardinality of such a set are posed as two open questions by Kim, Olesky and Driessche. In this note, the minimum cardinality of all critical sets of inertias for 4 × 4 irreducible zero-nonzero patterns is identified.

Keywords: Zero-nonzero pattern, inertia, critical set of inertias, inertially arbitrary.

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Authors: Shuenn-Yih Chang, Chiu-Li Huang

Abstract:

Three structure-dependent integration methods have been developed for solving equations of motion, which are second-order ordinary differential equations, for structural dynamics and earthquake engineering applications. Although they generally have the same numerical properties, such as explicit formulation, unconditional stability and second-order accuracy, a different performance is found in solving the free vibration response to either linear elastic or nonlinear systems with high frequency modes. The root cause of this different performance in the free vibration responses is analytically explored herein. As a result, it is verified that a weak instability is responsible for the different performance of the integration methods. In general, a weak instability will result in an inaccurate solution or even numerical instability in the free vibration responses of high frequency modes. As a result, a weak instability must be prohibited for time integration methods.

Keywords: Dynamic analysis, high frequency, integration method, overshoot, weak instability.

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Authors: Sheng-Ming Huang, Nan-Chyuan Tsai, Chih-Che Lin, Chun-Chi Lin

Abstract:

An innovative design for intelligent Chemical Mechanical Polishing (CMP) system is proposed and verified by experiments in this report. On-line measurement and real-time feedback are integrated to eliminate the shortcomings of traditional approaches, e.g., the batch-to-batch discrepancy of required polishing time, over consumption of chemical slurry, and non-uniformity across the wafer. The major advantage of the proposed method is that the finish of local surface roughness can be consistent, no matter where the inner-ring region or outer-ring region is concerned. Secondly, it is able to eliminate the Edge effect. Conventionally, the interfacial induced stress near the wafer edge is generally much higher than that near the wafer center. At last, by using the proposed intelligent chemical mechanical polishing strategy, the cost of the entire machining cycle can be much reduced while the quality of the finished goods certainly upgraded.

Keywords: Chemical Mechanical Polishing, Active Magnetic Actuator, On-Line Measurement.

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Authors: Ber-Lin Yu, Ting-Zhu Huang

Abstract:

If there exists a nonempty, proper subset S of the set of all (n + 1)(n + 2)/2 inertias such that S Ôèå i(A) is sufficient for any n × n zero-nonzero pattern A to be inertially arbitrary, then S is called a critical set of inertias for zero-nonzero patterns of order n. If no proper subset of S is a critical set, then S is called a minimal critical set of inertias. In [3], Kim, Olesky and Driessche identified all minimal critical sets of inertias for 2 × 2 zero-nonzero patterns. Identifying all minimal critical sets of inertias for n × n zero-nonzero patterns with n ≥ 3 is posed as an open question in [3]. In this paper, all minimal critical sets of inertias for 3 × 3 zero-nonzero patterns are identified. It is shown that the sets {(0, 0, 3), (3, 0, 0)}, {(0, 0, 3), (0, 3, 0)}, {(0, 0, 3), (0, 1, 2)}, {(0, 0, 3), (1, 0, 2)}, {(0, 0, 3), (2, 0, 1)} and {(0, 0, 3), (0, 2, 1)} are the only minimal critical sets of inertias for 3 × 3 irreducible zerononzero patterns.

Keywords: Permutation digraph, zero-nonzero pattern, irreducible pattern, critical set of inertias, inertially arbitrary.

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Authors: Guilan Huang

Abstract:

In 2013 and 2014, the U.S. Food and Drug Administration (FDA) collected data from selected fast food restaurants and full service restaurants for tracking changes in the occurrence of foodborne illness risk factors. This paper discussed how we customized spatial random sampling method by considering financial position and availability of FDA resources, and how we enriched restaurants data with location. Location information of restaurants provides opportunity for quantitatively determining random sampling within non-government units (e.g.: 240 kilometers around each data-collector). Spatial analysis also could optimize data-collectors’ work plans and resource allocation. Spatial analytic and processing platform helped us handling the spatial random sampling challenges. Our method fits in FDA’s ability to pinpoint features of foodservice establishments, and reduced both time and expense on data collection.

Keywords: Geospatial technology, restaurant, retail food risk factors study, spatial random sampling.

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Authors: Haode Huo, Jingjing He, Rui Kang, Xuefei Guan

Abstract:

This paper presents a study of Lamb wave damage diagnosis of composite delamination using instantaneous phase data. Numerical experiments are performed using the finite element method. Different sizes of delamination damages are modeled using finite element package ABAQUS. Lamb wave excitation and responses data are obtained using a pitch-catch configuration. Empirical mode decomposition is employed to extract the intrinsic mode functions (IMF). Hilbert–Huang Transform is applied to each of the resulting IMFs to obtain the instantaneous phase information. The baseline data for healthy plates are also generated using the same procedure. The size of delamination is correlated with the instantaneous phase change for damage diagnosis. It is observed that the unwrapped instantaneous phase of shows a consistent behavior with the increasing delamination size.

Keywords: Delamination, lamb wave, finite element method, EMD, instantaneous phase.

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Authors: Yen-Ting Wang, Pao-Cheng Lin, Chen-Fu Huang, Lung-Ching Liang, Alex J.Y. Lee

Abstract:

This study examined the effects of 8-week Pilates training program on limits of stability (LOS) and abdominal muscle strength in young dancers. Twenty-four female volunteered and randomly assigned as experimental group (EG) or control group (CG). All subjects received the same dance lessons but the EG underwent an extra Pilates mat exercises for 40 minutes, three times a week, for 8 weeks. LOS was evaluated by the Biodex Balance System and the abdominal strength was measured by 30/60 seconds sit-ups test. One factor ANCOVA was used to examine the differences between groups after training. The results showed that the overall LOS scores at levels 2/8 and the 30/60 seconds sit-ups for the EG group pre- and post-training were changed from 22/38 % to 31/51 % and 20/33 times to 24/42 times, respectively. The study demonstrated that 8-week Pilates training can improve the LOS performance and abdominal strength in young dancers.

Keywords: Balance, Core Strength Exercise Training, and Posture Stability.

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Authors: Chen-Yu Wang, Tsung-Chien Chen, Ming-Hui Lee, Jen-Feng Huang

Abstract:

In this paper, the innovative intelligent fuzzy weighted input estimation method (FWIEM) can be applied to the inverse heat transfer conduction problem (IHCP) to estimate the unknown time-varying heat flux efficiently as presented. The feasibility of this method can be verified by adopting the temperature measurement experiment. We would like to focus attention on the heat flux estimation to three kinds of samples (Copper, Iron and Steel/AISI 304) with the same 3mm thickness. The temperature measurements are then regarded as the inputs into the FWIEM to estimate the heat flux. The experiment results show that the proposed algorithm can estimate the unknown time-varying heat flux on-line.

Keywords: Fuzzy Weighted Input Estimation Method, IHCP andHeat Flux.

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Authors: Xin Kai, Juan Li, Sexin Huang, Zengliang Bai

Abstract:

Ethanol is generally used as a therapeutic reagent against Hepatocellular carcinoma (HCC or hepatoma) worldwide, as it can induce Hepatocellular carcinoma cell apoptosis at low concentration through a multifactorial process regulated by several unknown proteins. This paper provides a simple and available proteomic strategy for exploring differentially expressed proteins in the apoptotic pathway. The appropriate concentrations of ethanol required to induce HepG2 cell apoptosis were first assessed by MTT assay, Gisma and fluorescence staining. Next, the central proteins involved in the apoptosis pathway processs were determined using 2D-PAGE, SDS-PAGE, and bio-software analysis. Finally the downregulation of two proteins, AFP and survivin, were determined by immunocytochemistry and reverse transcriptase PCR (RT-PCR) technology. The simple, useful method demonstrated here provides a new approach to proteomic analysis in key bio-regulating process including proliferation, differentiation, apoptosis, immunity and metastasis.

Keywords: Hepatocellular carcinoma, Ethanol, Proteomics, survivin and AFP

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Authors: Khodamorad Nabaki, Jianhu Shen, Xiaodong Huang

Abstract:

This paper develops a method for considering the critical fatigue stress as a constraint in the Bi-directional Evolutionary Structural Optimization (BESO) method. Our aim is to reach an optimal design in which high cycle fatigue failure does not occur for a specific life time. The critical fatigue stress is calculated based on modified Goodman criteria and used as a stress constraint in our topology optimization problem. Since fatigue generally does not occur for compressive stresses, we use the p-norm approach of the stress measurement that considers the highest tensile principal stress in each point as stress measure to calculate the sensitivity numbers. The BESO method has been extended to minimize volume an object subjected to the critical fatigue stress constraint. The optimization results are compared with the results from the compliance minimization problem which shows clearly the merits of our newly developed approach.

Keywords: Topology optimization, BESO method, p-norm, fatigue constraint.

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