Search results for: Harris Extraction and SIFT Matching
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1083

Search results for: Harris Extraction and SIFT Matching

1053 Face Recognition Using Eigen face Coefficients and Principal Component Analysis

Authors: Parvinder S. Sandhu, Iqbaldeep Kaur, Amit Verma, Samriti Jindal, Inderpreet Kaur, Shilpi Kumari

Abstract:

Face Recognition is a field of multidimensional applications. A lot of work has been done, extensively on the most of details related to face recognition. This idea of face recognition using PCA is one of them. In this paper the PCA features for Feature extraction are used and matching is done for the face under consideration with the test image using Eigen face coefficients. The crux of the work lies in optimizing Euclidean distance and paving the way to test the same algorithm using Matlab which is an efficient tool having powerful user interface along with simplicity in representing complex images.

Keywords: Eigen Face, Multidimensional, Matching, PCA.

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1052 Human Verification in a Video Surveillance System Using Statistical Features

Authors: Sanpachai Huvanandana

Abstract:

A human verification system is presented in this paper. The system consists of several steps: background subtraction, thresholding, line connection, region growing, morphlogy, star skelatonization, feature extraction, feature matching, and decision making. The proposed system combines an advantage of star skeletonization and simple statistic features. A correlation matching and probability voting have been used for verification, followed by a logical operation in a decision making stage. The proposed system uses small number of features and the system reliability is convincing.

Keywords: Human verification, object recognition, videounderstanding, segmentation.

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1051 Automatic Vehicle Identification by Plate Recognition

Authors: Serkan Ozbay, Ergun Ercelebi

Abstract:

Automatic Vehicle Identification (AVI) has many applications in traffic systems (highway electronic toll collection, red light violation enforcement, border and customs checkpoints, etc.). License Plate Recognition is an effective form of AVI systems. In this study, a smart and simple algorithm is presented for vehicle-s license plate recognition system. The proposed algorithm consists of three major parts: Extraction of plate region, segmentation of characters and recognition of plate characters. For extracting the plate region, edge detection algorithms and smearing algorithms are used. In segmentation part, smearing algorithms, filtering and some morphological algorithms are used. And finally statistical based template matching is used for recognition of plate characters. The performance of the proposed algorithm has been tested on real images. Based on the experimental results, we noted that our algorithm shows superior performance in car license plate recognition.

Keywords: Character recognizer, license plate recognition, plate region extraction, segmentation, smearing, template matching.

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1050 Matching Current Search with Future Postings

Authors: Kim Nee Goh, Viknesh Kumar Naleyah

Abstract:

Online trading is an alternative to conventional shopping method. People trade goods which are new or pre-owned before. However, there are times when a user is not able to search the items wanted online. This is because the items may not be posted as yet, thus ending the search. Conventional search mechanism only works by searching and matching search criteria (requirement) with data available in a particular database. This research aims to match current search requirements with future postings. This would involve the time factor in the conventional search method. A Car Matching Alert System (CMAS) prototype was developed to test the matching algorithm. When a buyer-s search returns no result, the system saves the search and the buyer will be alerted if there is a match found based on future postings. The algorithm developed is useful and as it can be applied in other search context.

Keywords: Matching algorithm, online trading, search, future postings, car matching

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1049 Generation of Photo-Mosaic Images through Block Matching and Color Adjustment

Authors: Hae-Yeoun Lee

Abstract:

Mosaic refers to a technique that makes image by gathering lots of small materials in various colors. This paper presents an automatic algorithm that makes the photo-mosaic image using photos. The algorithm is composed of 4 steps: partition and feature extraction, block matching, redundancy removal and color adjustment. The input image is partitioned in the small block to extract feature. Each block is matched to find similar photo in database by comparing similarity with Euclidean difference between blocks. The intensity of the block is adjusted to enhance the similarity of image by replacing the value of light and darkness with that of relevant block. Further, the quality of image is improved by minimizing the redundancy of tiles in the adjacent blocks. Experimental results support that the proposed algorithm is excellent in quantitative analysis and qualitative analysis.

Keywords: Photo-mosaic, Euclidean distance, Block matching, Intensity adjustment.

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1048 A Developmental Survey of Local Stereo Matching Algorithms

Authors: André Smith, Amr Abdel-Dayem

Abstract:

This paper presents an overview of the history and development of stereo matching algorithms. Details from its inception, up to relatively recent techniques are described, noting challenges that have been surmounted across these past decades. Different components of these are explored, though focus is directed towards the local matching techniques. While global approaches have existed for some time, and demonstrated greater accuracy than their counterparts, they are generally quite slow. Many strides have been made more recently, allowing local methods to catch up in terms of accuracy, without sacrificing the overall performance.

Keywords: Developmental survey, local stereo matching, stereo correspondence.

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1047 Computing Maximum Uniquely Restricted Matchings in Restricted Interval Graphs

Authors: Swapnil Gupta, C. Pandu Rangan

Abstract:

A uniquely restricted matching is defined to be a matching M whose matched vertices induces a sub-graph which has only one perfect matching. In this paper, we make progress on the open question of the status of this problem on interval graphs (graphs obtained as the intersection graph of intervals on a line). We give an algorithm to compute maximum cardinality uniquely restricted matchings on certain sub-classes of interval graphs. We consider two sub-classes of interval graphs, the former contained in the latter, and give O(|E|^2) time algorithms for both of them. It is to be noted that both sub-classes are incomparable to proper interval graphs (graphs obtained as the intersection graph of intervals in which no interval completely contains another interval), on which the problem can be solved in polynomial time.

Keywords: Uniquely restricted matching, interval graph, design and analysis of algorithms, matching, induced matching, witness counting.

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1046 A Trends Analysis of Image Processing in Unmanned Aerial Vehicle

Authors: Jae-Neung Lee, Keun-Chang Kwak

Abstract:

This paper describes an analysis of domestic and international trends of image processing for data in UAV (unmanned aerial vehicle) and also explains about UAV and Quadcopter. Overseas examples of image processing using UAV include image processing for totaling the total numberof vehicles, edge/target detection, detection and evasion algorithm, image processing using SIFT(scale invariant features transform) matching, and application of median filter and thresholding. In Korea, many studies are underway including visualization of new urban buildings.

Keywords: Image Processing, UAV, Quadcopter, Target detection.

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1045 Complex Energy Signal Model for Digital Human Fingerprint Matching

Authors: Jason Zalev, Reza Sedaghat

Abstract:

This paper describes a complex energy signal model that is isomorphic with digital human fingerprint images. By using signal models, the problem of fingerprint matching is transformed into the signal processing problem of finding a correlation between two complex signals that differ by phase-rotation and time-scaling. A technique for minutiae matching that is independent of image translation, rotation and linear-scaling, and is resistant to missing minutiae is proposed. The method was tested using random data points. The results show that for matching prints the scaling and rotation angles are closely estimated and a stronger match will have a higher correlation.

Keywords: Affine Invariant, Fingerprint Recognition, Matching, Minutiae.

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1044 Mechanisms of Ginger Bioactive Compounds Extract Using Soxhlet and Accelerated Water Extraction

Authors: M. N. Azian, A. N. Ilia Anisa, Y. Iwai

Abstract:

The mechanism for extraction bioactive compounds from plant matrix is essential for optimizing the extraction process. As a benchmark technique, a soxhlet extraction has been utilized for discussing the mechanism and compared with an accelerated water extraction. The trends of both techniques show that the process involves extraction and degradation. The highest yields of 6-, 8-, 10-gingerols and 6-shogaol in soxhlet extraction were 13.948, 7.12, 10.312 and 2.306 mg/g, respectively. The optimum 6-, 8-, 10-gingerols and 6-shogaol extracted by the accelerated water extraction at 140oC were 68.97±3.95 mg/g at 3min, 18.98±3.04 mg/g at 5min, 5.167±2.35 mg/g at 3min and 14.57±6.27 mg/g at 3min, respectively. The effect of temperature at 3mins shows that the concentration of 6-shogaol increased rapidly as decreasing the recovery of 6-gingerol.

Keywords: Mechanism, bioactive compounds, soxhlet extraction, accelerated water extraction.

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1043 Fingerprint Verification System Using Minutiae Extraction Technique

Authors: Manvjeet Kaur, Mukhwinder Singh, Akshay Girdhar, Parvinder S. Sandhu

Abstract:

Most fingerprint recognition techniques are based on minutiae matching and have been well studied. However, this technology still suffers from problems associated with the handling of poor quality impressions. One problem besetting fingerprint matching is distortion. Distortion changes both geometric position and orientation, and leads to difficulties in establishing a match among multiple impressions acquired from the same finger tip. Marking all the minutiae accurately as well as rejecting false minutiae is another issue still under research. Our work has combined many methods to build a minutia extractor and a minutia matcher. The combination of multiple methods comes from a wide investigation into research papers. Also some novel changes like segmentation using Morphological operations, improved thinning, false minutiae removal methods, minutia marking with special considering the triple branch counting, minutia unification by decomposing a branch into three terminations, and matching in the unified x-y coordinate system after a two-step transformation are used in the work.

Keywords: Biometrics, Minutiae, Crossing number, False Accept Rate (FAR), False Reject Rate (FRR).

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1042 High-Accuracy Satellite Image Analysis and Rapid DSM Extraction for Urban Environment Evaluations (Tripoli-Libya)

Authors: Abdunaser Abduelmula, Maria Luisa M. Bastos, José A. Gonçalves

Abstract:

Modelling of the earth's surface and evaluation of urban environment, with 3D models, is an important research topic. New stereo capabilities of high resolution optical satellites images, such as the tri-stereo mode of Pleiades, combined with new image matching algorithms, are now available and can be applied in urban area analysis. In addition, photogrammetry software packages gained new, more efficient matching algorithms, such as SGM, as well as improved filters to deal with shadow areas, can achieve more dense and more precise results. This paper describes a comparison between 3D data extracted from tri-stereo and dual stereo satellite images, combined with pixel based matching and Wallis filter. The aim was to improve the accuracy of 3D models especially in urban areas, in order to assess if satellite images are appropriate for a rapid evaluation of urban environments. The results showed that 3D models achieved by Pleiades tri-stereo outperformed, both in terms of accuracy and detail, the result obtained from a Geo-eye pair. The assessment was made with reference digital surface models derived from high resolution aerial photography. This could mean that tri-stereo images can be successfully used for the proposed urban change analyses.

Keywords: 3D Models, Environment, Matching, Pleiades.

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1041 An Experiment of Three-Dimensional Point Clouds Using GoPro

Authors: Jong-hwa Kim, Mu-wook Pyeon, Yang-dam Eo, Ill-woong Jang

Abstract:

Construction of geo-spatial information recently tends to develop as multi-dimensional geo-spatial information. People constructing spatial information is also expanding its area to the general public from some experts. As well as, studies are in progress using a variety of devices, with the aim of near real-time update. In this paper, getting the stereo images using GoPro device used widely also to the general public as well as experts. And correcting the distortion of the images, then by using SIFT, DLT, is acquired the point clouds. It presented a possibility that on the basis of this experiment, using a video device that is readily available in real life, to create a real-time digital map.

Keywords: GoPro, SIFT, DLT, Point Clouds.

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1040 An Index based Forward Backward Multiple Pattern Matching Algorithm

Authors: Raju Bhukya, DVLN Somayajulu

Abstract:

Pattern matching is one of the fundamental applications in molecular biology. Searching DNA related data is a common activity for molecular biologists. In this paper we explore the applicability of a new pattern matching technique called Index based Forward Backward Multiple Pattern Matching algorithm(IFBMPM), for DNA Sequences. Our approach avoids unnecessary comparisons in the DNA Sequence due to this; the number of comparisons of the proposed algorithm is very less compared to other existing popular methods. The number of comparisons rapidly decreases and execution time decreases accordingly and shows better performance.

Keywords: Comparisons, DNA Sequence, Index.

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1039 A Comparison and Analysis of Name Matching Algorithms

Authors: Chakkrit Snae

Abstract:

Names are important in many societies, even in technologically oriented ones which use e.g. ID systems to identify individual people. Names such as surnames are the most important as they are used in many processes, such as identifying of people and genealogical research. On the other hand variation of names can be a major problem for the identification and search for people, e.g. web search or security reasons. Name matching presumes a-priori that the recorded name written in one alphabet reflects the phonetic identity of two samples or some transcription error in copying a previously recorded name. We add to this the lode that the two names imply the same person. This paper describes name variations and some basic description of various name matching algorithms developed to overcome name variation and to find reasonable variants of names which can be used to further increasing mismatches for record linkage and name search. The implementation contains algorithms for computing a range of fuzzy matching based on different types of algorithms, e.g. composite and hybrid methods and allowing us to test and measure algorithms for accuracy. NYSIIS, LIG2 and Phonex have been shown to perform well and provided sufficient flexibility to be included in the linkage/matching process for optimising name searching.

Keywords: Data mining, name matching algorithm, nominaldata, searching system.

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1038 Extraction Condition of Echinocactus grusonii

Authors: R. Oonsivilai, N. Chaijareonudomroung, Y. Huantanom, A. Oonsivilai

Abstract:

The optimal extraction condition of dried Echinocactus grusonii powder was studied. The three independent variables are raw material drying temperature, extraction temperature, and extraction time. The dependent variables are both yield percentage of crude extract and total phenolic quantification as gallic acid equivalent in crude extract. The experimental design was based on central composite design. Highest yield percentage of crude extract could get from extraction condition at raw material drying temperature at 60°C, extraction temperature at 15°C, and extraction time for 25 min °C. Moreover, the crude extract with highest phenolic occurred by extraction condition of raw material drying temperature at 60°C, extraction temperature at 35 °C, and extraction lasting 25 min.

Keywords: Drying temperature, Extraction temperature, Optimal condition, Total phenolic

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1037 A Practical and Efficient Evaluation Function for 3D Model Based Vehicle Matching

Authors: Yuan Zheng

Abstract:

3D model-based vehicle matching provides a new way for vehicle recognition, localization and tracking. Its key is to construct an evaluation function, also called fitness function, to measure the degree of vehicle matching. The existing fitness functions often poorly perform when the clutter and occlusion exist in traffic scenarios. In this paper, we present a practical and efficient fitness function. Unlike the existing evaluation functions, the proposed fitness function is to study the vehicle matching problem from both local and global perspectives, which exploits the pixel gradient information as well as the silhouette information. In view of the discrepancy between 3D vehicle model and real vehicle, a weighting strategy is introduced to differently treat the fitting of the model’s wireframes. Additionally, a normalization operation for the model’s projection is performed to improve the accuracy of the matching. Experimental results on real traffic videos reveal that the proposed fitness function is efficient and robust to the cluttered background and partial occlusion.

Keywords: 3D-2D matching, fitness function, 3D vehicle model, local image gradient, silhouette information.

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1036 Improved Feature Processing for Iris Biometric Authentication System

Authors: Somnath Dey, Debasis Samanta

Abstract:

Iris-based biometric authentication is gaining importance in recent times. Iris biometric processing however, is a complex process and computationally very expensive. In the overall processing of iris biometric in an iris-based biometric authentication system, feature processing is an important task. In feature processing, we extract iris features, which are ultimately used in matching. Since there is a large number of iris features and computational time increases as the number of features increases, it is therefore a challenge to develop an iris processing system with as few as possible number of features and at the same time without compromising the correctness. In this paper, we address this issue and present an approach to feature extraction and feature matching process. We apply Daubechies D4 wavelet with 4 levels to extract features from iris images. These features are encoded with 2 bits by quantizing into 4 quantization levels. With our proposed approach it is possible to represent an iris template with only 304 bits, whereas existing approaches require as many as 1024 bits. In addition, we assign different weights to different iris region to compare two iris templates which significantly increases the accuracy. Further, we match the iris template based on a weighted similarity measure. Experimental results on several iris databases substantiate the efficacy of our approach.

Keywords: Iris recognition, biometric, feature processing, patternrecognition, pattern matching.

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1035 On Phase Based Stereo Matching and Its Related Issues

Authors: Andr´as R¨ovid, Takeshi Hashimoto

Abstract:

The paper focuses on the problem of the point correspondence matching in stereo images. The proposed matching algorithm is based on the combination of simpler methods such as normalized sum of squared differences (NSSD) and a more complex phase correlation based approach, by considering the noise and other factors, as well. The speed of NSSD and the preciseness of the phase correlation together yield an efficient approach to find the best candidate point with sub-pixel accuracy in stereo image pairs. The task of the NSSD in this case is to approach the candidate pixel roughly. Afterwards the location of the candidate is refined by an enhanced phase correlation based method which in contrast to the NSSD has to run only once for each selected pixel.

Keywords: Stereo matching, Sub-pixel accuracy, phase correlation, SVD, NSSD.

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1034 CFD Simulation of Dense Gas Extraction through Polymeric Membranes

Authors: Azam Marjani, Saeed Shirazian

Abstract:

In this study is presented a general methodology to predict the performance of a continuous near-critical fluid extraction process to remove compounds from aqueous solutions using hollow fiber membrane contactors. A comprehensive 2D mathematical model was developed to study Porocritical extraction process. The system studied in this work is a membrane based extractor of ethanol and acetone from aqueous solutions using near-critical CO2. Predictions of extraction percentages obtained by simulations have been compared to the experimental values reported by Bothun et al. [5]. Simulations of extraction percentage of ethanol and acetone show an average difference of 9.3% and 6.5% with the experimental data, respectively. More accurate predictions of the extraction of acetone could be explained by a better estimation of the transport properties in the aqueous phase that controls the extraction of this solute.

Keywords: Solvent extraction, Membrane, Mass transfer, Densegas, Modeling

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1033 Chinese Event Detection Technique Based on Dependency Parsing and Rule Matching

Authors: Weitao Lin

Abstract:

To quickly extract adequate information from large-scale unstructured text data, this paper studies the representation of events in Chinese scenarios and performs the regularized abstraction. It proposes a Chinese event detection technique based on dependency parsing and rule matching. The method first performs dependency parsing on the original utterance, then performs pattern matching at the word or phrase granularity based on the results of dependent syntactic analysis, filters out the utterances with prominent non-event characteristics, and obtains the final results. The experimental results show the effectiveness of the method.

Keywords: Natural Language Processing, Chinese event detection, rules matching, dependency parsing.

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1032 Thermodynamic Study of Seed Oil Extraction by Organic Solvents

Authors: Zhila Safari, Ali Ashrafizadeh, Najaf Hedayat

Abstract:

Thermodynamics characterization Sesame oil extraction by Acetone, Hexane and Benzene has been evaluated. The 120 hours experimental Data were described by a simple mathematical model. According to the simulation results and the essential criteria, Acetone is superior to other solvents but under certain conditions where oil extraction takes place Hexane is superior catalyst.

Keywords: Liquid-solid extraction, seed oil, ThermodynamicStudy.

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1031 Effect of Enzyme and Heat Pretreatment on Sunflower Oil Recovery Using Aqueous and Hexane Extractions

Authors: E. Danso-Boateng

Abstract:

The effects of enzyme action and heat pretreatment on oil extraction yield from sunflower kernels were analysed using hexane extraction with Soxhlet, and aqueous extraction with incubator shaker. Ground kernels of raw and heat treated kernels, each with and without Viscozyme treatment were used. Microscopic images of the kernels were taken to analyse the visible effects of each treatment on the cotyledon cell structure of the kernels. Heat pretreated kernels before both extraction processes produced enhanced oil extraction yields than the control, with steam explosion the most efficient. In hexane extraction, applying a combination of steam explosion and Viscozyme treatments to the kernels before the extraction gave the maximum oil extractable in 1 hour; while for aqueous extraction, raw kernels treated with Viscozyme gave the highest oil extraction yield. Remarkable cotyledon cell disruption was evident in kernels treated with Viscozyme; whereas steam explosion and conventional heat treated kernels had similar effects.

Keywords: Enzyme-assisted aqueous and hexane extraction, heatpretreatment, sunflower cotyledon structure, sunflower oil extraction

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1030 Subcritical Water Extraction of Mannitol from Olive Leaves

Authors: S. M. Ghoreishi, R. Gholami Shahrestani, S. H. Ghaziaskar

Abstract:

Subcritical water extraction was investigated as a novel and alternative technology in the food and pharmaceutical industry for the separation of Mannitol from olive leaves and its results was compared with those of Soxhlet extraction. The effects of temperature, pressure, and flow rate of water and also momentum and mass transfer dimensionless variables such as Reynolds and Peclet Numbers on extraction yield and equilibrium partition coefficient were investigated. The 30-110 bars, 60-150°C, and flow rates of 0.2-2 mL/min were the water operating conditions. The results revealed that the highest Mannitol yield was obtained at 100°C and 50 bars. However, extraction of Mannitol was not influenced by the variations of flow rate. The mathematical modeling of experimental measurements was also investigated and the model is capable of predicting the experimental measurements very well. In addition, the results indicated higher extraction yield for the subcritical water extraction in contrast to Soxhlet method.

Keywords: Extraction, Mannitol, Modeling, Olive leaves, Soxhlet extraction, Subcritical water.

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1029 Driver Fatigue State Recognition with Pixel Based Caveat Scheme Using Eye-Tracking

Authors: K. Thulasimani, K. G. Srinivasagan

Abstract:

Driver fatigue is an important factor in the increasing number of road accidents. Dynamic template matching method was proposed to address the problem of real-time driver fatigue detection system based on eye-tracking. An effective vision based approach was used to analyze the driver’s eye state to detect fatigue. The driver fatigue system consists of Face detection, Eye detection, Eye tracking, and Fatigue detection. Initially frames are captured from a color video in a car dashboard and transformed from RGB into YCbCr color space to detect the driver’s face. Canny edge operator was used to estimating the eye region and the locations of eyes are extracted. The extracted eyes were considered as a template matching for eye tracking. Edge Map Overlapping (EMO) and Edge Pixel Count (EPC) matching function were used for eye tracking which is used to improve the matching accuracy. The pixel of eyeball was tracked from the eye regions which are used to determine the fatigue state of the driver.

Keywords: Driver fatigue detection, Driving safety, Eye tracking, Intelligent transportation system, Template matching.

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1028 Pakistan Sign Language Recognition Using Statistical Template Matching

Authors: Aleem Khalid Alvi, M. Yousuf Bin Azhar, Mehmood Usman, Suleman Mumtaz, Sameer Rafiq, RaziUr Rehman, Israr Ahmed

Abstract:

Sign language recognition has been a topic of research since the first data glove was developed. Many researchers have attempted to recognize sign language through various techniques. However none of them have ventured into the area of Pakistan Sign Language (PSL). The Boltay Haath project aims at recognizing PSL gestures using Statistical Template Matching. The primary input device is the DataGlove5 developed by 5DT. Alternative approaches use camera-based recognition which, being sensitive to environmental changes are not always a good choice.This paper explains the use of Statistical Template Matching for gesture recognition in Boltay Haath. The system recognizes one handed alphabet signs from PSL.

Keywords: Gesture Recognition, Pakistan Sign Language, DataGlove, Human Computer Interaction, Template Matching, BoltayHaath

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1027 Similarity Measure Functions for Strategy-Based Biometrics

Authors: Roman V. Yampolskiy, Venu Govindaraju

Abstract:

Functioning of a biometric system in large part depends on the performance of the similarity measure function. Frequently a generalized similarity distance measure function such as Euclidian distance or Mahalanobis distance is applied to the task of matching biometric feature vectors. However, often accuracy of a biometric system can be greatly improved by designing a customized matching algorithm optimized for a particular biometric application. In this paper we propose a tailored similarity measure function for behavioral biometric systems based on the expert knowledge of the feature level data in the domain. We compare performance of a proposed matching algorithm to that of other well known similarity distance functions and demonstrate its superiority with respect to the chosen domain.

Keywords: Behavioral Biometrics, Euclidian Distance, Matching, Similarity Measure.

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1026 Effect of Wheat Flour Extraction Rates on Flour Composition, Farinographic Characteristics and Sensory Perception of Sourdough Naans

Authors: Ghulam Mueen-ud-Din, Salim-ur-Rehman, Faqir M. Anjum, Haq Nawaz, Mian A. Murtaza

Abstract:

The effect of wheat flour extraction rates on flour composition, farinographic characteristics and the quality of sourdough naans was investigated. The results indicated that by increasing the extraction rate, the amount of protein, fiber, fat and ash increased, whereas moisture content decreased. Farinographic characteristic like water absorption and dough development time increased with an increase in flour extraction rate but the dough stabilities and tolerance indices were reduced with an increase in flour extraction rates. Titratable acidity for both sourdough and sourdough naans also increased along with flour extraction rate. The study showed that overall quality of sourdough naans were affected by both flour extraction rate and starter culture used. Sensory analysis of sourdough naans revealed that desirable extraction rate for sourdough naan was 76%.

Keywords: Extraction rates, Farinographic characteristics, Flour composition, Sourdough naans, Wheat flour.

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1025 Extraction Condition of Phaseolus vulgaris

Authors: Ratchadaporn Oonsivilai, Jutarat Manatwiyangkool, Anant Oonsivilai

Abstract:

Theoptimal extraction condition of dried Phaseolus vulgaris powderwas studied. The three independent variables are raw material concentration, shaking and centrifugaltime. The dependent variables are both yield percentage of crude extract and alphaamylase enzyme inhibition activity. The experimental design was based on box-behnkendesign. Highest yield percentage of crude extract could get from extraction condition at concentration of 1, 0,1, concentration of 0.15 M ,extraction time for 2hour, and separationtime for60 min. Moreover, the crude extract with highest alpha-amylase enzyme inhibition activityoccurred by extraction condition at concentration of 0.10 M, extraction time for 2 min, and separation time for 45 min

Keywords: Extraction time, Optimal condition, Alpha-amylase enzymeinhibition activity

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1024 A New Method for Rapid DNA Extraction from Artemia (Branchiopoda, Crustacea)

Authors: R. Manaffar, R. Maleki, S. Zare, N. Agh, S. Soltanian, B. Sehatnia, P. Sorgeloos, P. Bossier, G. Van Stappen

Abstract:

Artemia is one of the most conspicuous invertebrates associated with aquaculture. It can be considered as a model organism, offering numerous advantages for comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is an important step of any molecular experiment, a new and a rapid method of DNA extraction from adult Artemia was described in this study. Besides, the efficiency of this technique was compared with two widely used alternative techniques, namely Chelex® 100 resin and SDS-chloroform methods. Data analysis revealed that the new method is the easiest and the most cost effective method among the other methods which allows a quick and efficient extraction of DNA from the adult animal.

Keywords: APD, Artemia, DNA extraction, Molecularexperiments

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