Search results for: Active Radio Frequency Identification
1258 A DNA-Based Nanobiosensor for the Rapid Detection of the Dengue Virus in Mosquito
Authors: Lilia M. Fernando, Matthew K. Vasher, Evangelyn C. Alocilja
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This paper describes the development of a DNA-based nanobiosensor to detect the dengue virus in mosquito using electrically active magnetic (EAM) nanoparticles as concentrator and electrochemical transducer. The biosensor detection encompasses two sets of oligonucleotide probes that are specific to the dengue virus: the detector probe labeled with the EAM nanoparticles and the biotinylated capture probe. The DNA targets are double hybridized to the detector and the capture probes and concentrated from nonspecific DNA fragments by applying a magnetic field. Subsequently, the DNA sandwiched targets (EAM-detector probe– DNA target–capture probe-biotin) are captured on streptavidin modified screen printed carbon electrodes through the biotinylated capture probes. Detection is achieved electrochemically by measuring the oxidation–reduction signal of the EAM nanoparticles. Results indicate that the biosensor is able to detect the redox signal of the EAM nanoparticles at dengue DNA concentrations as low as 10 ng/μl.Keywords: Dengue, magnetic nanoparticles, mosquito, nanobiosensor.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 28611257 Comparison of the Parameter using ECG with Bisepctrum Parameter using EEG during General Anesthesia
Authors: Seong-wan Baik, Soo-young Ye, Byeong-cheol Choi, Gye-rok Jeon
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The measurement of anesthetic depth is necessary in anesthesiology. NN10 is very simple method among the RR intervals analysis methods. NN10 parameter means the numbers of above the 10 ms intervals of the normal to normal RR intervals. Bispectrum analysis is defined as 2D FFT. EEG signal reflected the non-linear peristalsis phenomena according to the change brain function. After analyzing the bispectrum of the 2 dimension, the most significant power spectrum density peaks appeared abundantly at the specific area in awakening and anesthesia state. These points are utilized to create the new index since many peaks appeared at the specific area in the frequency coordinate. The measured range of an index was 0-100. An index is 20-50 at an anesthesia, while the index is 90-60 at the awake. In this paper, the relation between NN10 parameter using ECG and bisepctrum index using EEG is observed to estimate the depth of anesthesia during anesthesia and then we estimated the utility of the anesthetic.Keywords: Anesthesia, Bispectrum index, ECG, EEG
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15741256 How to Improve Teaching and Learning Strategies through Educational Research: An Experience of Peer Observation in Legal Education
Authors: L. Mortari, A. Bevilacqua, R. Silva
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The experience presented in this paper aims to understand how educational research can support the introduction and optimization of teaching innovations in legal education. In this increasingly complex context, a strong need to introduce paths aimed at acquiring not only professional knowledge and skills but also reflective, critical and problem-solving skills emerges. Through a peer observation intertwined with an analysis of discursive practices, researchers and the teacher worked together through a process of participatory and transformative accompaniment whose objective was to promote the active participation and engagement of students in learning processes, an element indispensable to work in the more specific direction of strengthening key competences. This reflective faculty development path led the teacher to activate metacognitive processes, becoming thus aware of the strengths and areas of improvement of his teaching innovation.
Keywords: Discursive analysis, faculty development, legal education, peer observation, teaching innovation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3571255 Investigation of Interference Conditions in BFWA System Applying Adaptive TDD
Authors: Gábor Szládek, Balázs Héder, János Bitó
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In a BFWA (Broadband Fixed Wireless Access Network) the evolved SINR (Signal to Interference plus Noise Ratio) is relevant influenced by the applied duplex method. The TDD (Time Division Duplex), especially adaptive TDD method has some advantage contrary to FDD (Frequency Division Duplex), for example the spectrum efficiency and flexibility. However these methods are suffering several new interference situations that can-t occur in a FDD system. This leads to reduced SINR in the covered area what could cause some connection outages. Therefore, countermeasure techniques against interference are necessary to apply in TDD systems. Synchronization is one way to handling the interference. In this paper the TDD systems – applying different system synchronization degree - will be compared by the evolved SINR at different locations of the BFWA service area and the percentage of the covered area by the system.
Keywords: Adaptive TDD, BFWA networks, duplex methods, intra system interferences.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 13291254 Fractal Analysis on Human Colonic Pressure Activities based on the Box-counting Method
Authors: Rongguo Yan, Guozheng Yan, Banghua Yang
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The colonic tissue is a complicated dynamic system and the colonic activities it generates are composed of irregular segmental waves, which are referred to as erratic fluctuations or spikes. They are also highly irregular with subunit fractal structure. The traditional time-frequency domain statistics like the averaged amplitude, the motility index and the power spectrum, etc. are insufficient to describe such fluctuations. Thus the fractal box-counting dimension is proposed and the fractal scaling behaviors of the human colonic pressure activities under the physiological conditions are studied. It is shown that the dimension of the resting activity is smaller than that of the normal one, whereas the clipped version, which corresponds to the activity of the constipation patient, shows with higher fractal dimension. It may indicate a practical application to assess the colonic motility, which is often indicated by the colonic pressure activity.Keywords: Colonic pressure activity, erratic fluctuations, fractal dimension and spikes.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15091253 The Relationship between Citizenship Acquisition and Ethnic Identity of Immigrant Women in Taiwan
Authors: Yuan-Yu Chiang, Yu-Han Tseng, Chin-Chen Wen
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In the last few decades, many southeast-Asia women migrate to Taiwan by marriage, and it usually takes several years for them to acquire Taiwanese citizenship. This study investigates the relationship between their citizenship acquisition and whether they develop Taiwanese identities, and how does it affect their ethnical identity towards their original ethnics. Furthermore, the present study also explores that whether citizenship acquisition help the immigrant women to explore the host society further and make commitment to it, or the identification towards mainstream Taiwanese society is only symbolic and superficial? One hundred and ninety-two immigrant women were measured using Multigroup Ethnic Identity Measure-Revised and a global 10-point ethnic identity question. Correlation tests, t-test, and hierarchical regression were performed to answer the above questions. The results revealed that citizenship acquisition does help immigrant women to identify with Taiwanese society, but it does not affect how they identify with their own ethnics. Furthermore, the results also indicated that acquiring citizenship would not help these immigrant women become involved in deeper cultural exploration of Taiwan nor would it encourage them to make commitments to the host society.
Keywords: Immigrants, international marriage, ethnic identity, Taiwan.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16571252 Assessment of Influence of Short-Lasting Whole-Body Vibration on Joint Position Sense and Body Balance–A Randomised Masked Study
Authors: Anna Słupik, Anna Mosiołek, Sebastian Wójtowicz, Dariusz Białoszewski
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Introduction: Whole-Body Vibration (WBV) uses high frequency mechanical stimuli generated by a vibration plate and transmitted through bone, muscle and connective tissues to the whole body. Research has shown that long-term vibration-plate training improves neuromuscular facilitation, especially in afferent neural pathways, responsible for the conduction of vibration and proprioceptive stimuli, muscle function, balance and proprioception. Some researchers suggest that the vibration stimulus briefly inhibits the conduction of afferent signals from proprioceptors and can interfere with the maintenance of body balance. The aim of this study was to evaluate the influence of a single set of exercises associated with whole-body vibration on the joint position sense and body balance. Material and methods: The study enrolled 55 people aged 19-24 years. These individuals were randomly divided into a test group (30 persons) and a control group (25 persons). Both groups performed the same set of exercises on a vibration plate. The following vibration parameters: frequency of 20Hz and amplitude of 3mm, were used in the test group. The control group performed exercises on the vibration plate while it was off. All participants were instructed to perform six dynamic exercises lasting 30 seconds each with a 60-second period of rest between them. The exercises involved large muscle groups of the trunk, pelvis and lower limbs. Measurements were carried out before and immediately after exercise. Joint position sense (JPS) was measured in the knee joint for the starting position at 45° in an open kinematic chain. JPS error was measured using a digital inclinometer. Balance was assessed in a standing position with both feet on the ground with the eyes open and closed (each test lasting 30 sec). Balance was assessed using Matscan with FootMat 7.0 SAM software. The surface of the ellipse of confidence and front-back as well as right-left swing were measured to assess balance. Statistical analysis was performed using Statistica 10.0 PL software. Results: There were no significant differences between the groups, both before and after the exercise (p> 0.05). JPS did not change in both the test (10.7° vs. 8.4°) and control groups (9.0° vs. 8.4°). No significant differences were shown in any of the test parameters during balance tests with the eyes open or closed in both the test and control groups (p> 0.05). Conclusions: 1. Deterioration in proprioception or balance was not observed immediately after the vibration stimulus. This suggests that vibrationinduced blockage of proprioceptive stimuli conduction can have only a short-lasting effect that occurs only as long as a vibration stimulus is present. 2. Short-term use of vibration in treatment does not impair proprioception and seems to be safe for patients with proprioceptive impairment. 3. These results need to be supplemented with an assessment of proprioception during the application of vibration stimuli. Additionally, the impact of vibration parameters used in the exercises should be evaluated.Keywords: Balance, joint position sense, proprioception, whole body vibration.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16091251 Active Surface Tracking Algorithm for All-Fiber Common-Path Fourier-Domain Optical Coherence Tomography
Authors: Bang Young Kim, Sang Hoon Park, Chul Gyu Song
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A conventional optical coherence tomography (OCT) system has limited imaging depth, which is 1-2 mm, and suffers unwanted noise such as speckle noise. The motorized-stage-based OCT system, using a common-path Fourier-domain optical coherence tomography (CP-FD-OCT) configuration, provides enhanced imaging depth and less noise so that we can overcome these limitations. Using this OCT systems, OCT images were obtained from an onion, and their subsurface structure was observed. As a result, the images obtained using the developed motorized-stage-based system showed enhanced imaging depth than the conventional system, since it is real-time accurate depth tracking. Consequently, the developed CP-FD-OCT systems and algorithms have good potential for the further development of endoscopic OCT for microsurgery.Keywords: Common-path OCT, FD-OCT, OCT, Tracking algorithm.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16631250 A Novel Implementation of Application Specific Instruction-set Processor (ASIP) using Verilog
Authors: Kamaraju.M, Lal Kishore.K, Tilak.A.V.N
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The general purpose processors that are used in embedded systems must support constraints like execution time, power consumption, code size and so on. On the other hand an Application Specific Instruction-set Processor (ASIP) has advantages in terms of power consumption, performance and flexibility. In this paper, a 16-bit Application Specific Instruction-set processor for the sensor data transfer is proposed. The designed processor architecture consists of on-chip transmitter and receiver modules along with the processing and controlling units to enable the data transmission and reception on a single die. The data transfer is accomplished with less number of instructions as compared with the general purpose processor. The ASIP core operates at a maximum clock frequency of 1.132GHz with a delay of 0.883ns and consumes 569.63mW power at an operating voltage of 1.2V. The ASIP is implemented in Verilog HDL using the Xilinx platform on Virtex4.Keywords: ASIP, Data transfer, Instruction set, Processor
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20681249 A Novel Approach to Improve Users Search Goal in Web Usage Mining
Authors: R. Lokeshkumar, P. Sengottuvelan
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Web mining is to discover and extract useful Information. Different users may have different search goals when they search by giving queries and submitting it to a search engine. The inference and analysis of user search goals can be very useful for providing an experience result for a user search query. In this project, we propose a novel approach to infer user search goals by analyzing search web logs. First, we propose a novel approach to infer user search goals by analyzing search engine query logs, the feedback sessions are constructed from user click-through logs and it efficiently reflect the information needed for users. Second we propose a preprocessing technique to clean the unnecessary data’s from web log file (feedback session). Third we propose a technique to generate pseudo-documents to representation of feedback sessions for clustering. Finally we implement k-medoids clustering algorithm to discover different user search goals and to provide a more optimal result for a search query based on feedback sessions for the user.Keywords: Data Preprocessing, Session Identification, Web log mining, Web Personalization.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20231248 Investigation of Utilizing L-Band Horn Antenna in Landmine Detection
Authors: Ahmad H. Abdelgwad, Ahmed A. Nashat
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Landmine detection is an important and yet challenging problem remains to be solved. Ground Penetrating Radar (GPR) is a powerful and rapidly maturing technology for subsurface threat identification. The detection methodology of GPR depends mainly on the contrast of the dielectric properties of the searched target and its surrounding soil. This contrast produces a partial reflection of the electromagnetic pulses that are being transmitted into the soil and then being collected by the GPR. One of the most critical hardware components for the performance of GPR is the antenna system. The current paper explores the design and simulation of a pyramidal horn antenna operating at L-band frequencies (1- 2 GHz) to detect a landmine. A prototype model of the GPR system setup is developed to simulate full wave analysis of the electromagnetic fields in different soil types. The contrast in the dielectric permittivity of the landmine and the sandy soil is the most important parameter to be considered for detecting the presence of landmine. L-band horn antenna is proved to be well-versed in the investigation of landmine detection.
Keywords: Full wave analysis, ground penetrating radar, horn antenna design, landmine detection.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10031247 M-ary Chaotic Sequence Based SLM-OFDM System for PAPR Reduction without Side-Information
Authors: A.Goel, M. Agrawal, P. Gupta Poddar
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Selected Mapping (SLM) is a PAPR reduction technique, which converts the OFDM signal into several independent signals by multiplication with the phase sequence set and transmits one of the signals with lowest PAPR. But it requires the index of the selected signal i.e. side information (SI) to be transmitted with each OFDM symbol. The PAPR reduction capability of the SLM scheme depends on the selection of phase sequence set. In this paper, we have proposed a new phase sequence set generation scheme based on M-ary chaotic sequence and a mapping scheme to map quaternary data to concentric circle constellation (CCC) is used. It is shown that this method does not require SI and provides better SER performance with good PAPR reduction capability as compared to existing SLMOFDM methods.
Keywords: Orthogonal frequency division multiplexing (OFDM), Peak-to-average power ratio (PAPR), Selected mapping (SLM), Side information (SI)
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19601246 Immobilization of Lipase Enzyme by Low Cost Material: A Statistical Approach
Authors: Md. Z. Alam, Devi R. Asih, Md. N. Salleh
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Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-factor-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by the response surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/g-AC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05.
Keywords: Activated carbon, adsorption, immobilization, POME based lipase.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 25751245 Improving Protein-Protein Interaction Prediction by Using Encoding Strategies and Random Indices
Authors: Essam Al-Daoud
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A New features are extracted and compared to improve the prediction of protein-protein interactions. The basic idea is to select and use the best set of features from the Tensor matrices that are produced by the frequency vectors of the protein sequences. Three set of features are compared, the first set is based on the indices that are the most common in the interacting proteins, the second set is based on the indices that tend to be common in the interacting and non-interacting proteins, and the third set is constructed by using random indices. Moreover, three encoding strategies are compared; that are based on the amino asides polarity, structure, and chemical properties. The experimental results indicate that the highest accuracy can be obtained by using random indices with chemical properties encoding strategy and support vector machine.Keywords: protein-protein interactions, random indices, encoding strategies, support vector machine.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15671244 Implementing a Mobility Platform to Connect Hubs in Rural Areas
Authors: E. Neidhardt
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Mobility, for many people, is an important factor in the satisfaction of daily needs and many people are dependent on public transport. In rural areas with a low population density, it is difficult to provide public transportation with sufficient coverage and frequency. Therefore, the available public transport is unattractive. As a result, people use their own car, which is not desirable from a sustainable point of view and not possible for children and elderly people. Sometimes people organize themselves and volunteer transport services are created. These services are similar to demand-oriented taxis. However, these transport services are usually independent from each other and from the available line-based public transport, limiting both their usability and sustainability. We have developed a platform to improve usability and sustainability by connecting the different demand-oriented transport offerings with the line-based public transport. The system was implemented and tested in a rural area in Germany, but the SARS-CoV-2 pandemic limited real live operation.
Keywords: demand-oriented, HubChain, living lab, public transport
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3701243 Using ANSYS to Realize a Semi-Analytical Method for Predicting Temperature Profile in Injection/Production Well
Authors: N. Tarom, M.M. Hossain
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Determination of wellbore problems during a production/injection process might be evaluated thorough temperature log analysis. Other applications of this kind of log analysis may also include evaluation of fluid distribution analysis along the wellbore and identification of anomalies encountered during production/injection process. While the accuracy of such prediction is paramount, the common method of determination of a wellbore temperature log includes use of steady-state energy balance equations, which hardly describe the real conditions as observed in typical oil and gas flowing wells during production operation; and thus increase level of uncertainties. In this study, a practical method has been proposed through development of a simplified semianalytical model to apply for predicting temperature profile along the wellbore. The developed model includes an overall heat transfer coefficient accounting all modes of heat transferring mechanism, which has been focused on the prediction of a temperature profile as a function of depth for the injection/production wells. The model has been validated with the results obtained from numerical simulation.Keywords: Energy balance equation, reservoir and well performance, temperature log, overall heat transfer coefficient.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 27351242 Effect of the Support Shape on Fischer-Tropsch Cobalt Catalyst Performance
Authors: Jian Huang, Weixin Qian, Hongfang Ma, Haitao Zhang, Weiyong Ying
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Cobalt catalysts were supported on extruded silica carrier and different-type (SiO2, γ-Al2O3) commercial supports with different shapes and sizes to produce heavy hydrocarbons for Fischer-Tropsch synthesis. The catalysts were characterized by N2 physisorption and H2-TPR. The catalytic performance of the catalysts was tested in a fixed bed reactor. The results of Fischer-Tropsch synthesis performance showed that the cobalt catalyst supported on spherical silica supports displayed a higher activity and a higher selectivity to C5+ products, due to the fact that the active components were only distributed in the surface layer of spherical carrier, and the influence of gas diffusion restriction on catalytic performance was weakened. Therefore, it can be concluded that the eggshell cobalt catalyst was superior to precious metals modified catalysts in the synthesis of heavy hydrocarbons.
Keywords: Fischer-Tropsch synthesis, cobalt catalyst, support shape, heavy hydrocarbons.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 7651241 The Comparison of Form Drag and Profile Dragof a Wind Turbine Blade Section in Pitching Oscillation
Authors: M. R. Soltani, M. Seddighi, M. Mahmoudi
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Extensive wind tunnel tests have been conducted to investigate the unsteady flow field over and behind a 2D model of a 660 kW wind turbine blade section in pitching motion. The surface pressure and wake dynamic pressure variation at a distance of 1.5 chord length from trailing edge were measured by pressure transducers during several oscillating cycles at 3 reduced frequencies and oscillating amplitudes. Moreover, form drag and linear momentum deficit are extracted and compared at various conditions. The results show that the wake velocity field and surface pressure of the model have similar behavior before and after the airfoil beyond the static stall angle of attack. In addition, the effects of reduced frequency and oscillation amplitudes are discussed.Keywords: Pitching motion, form drag, Profile drag, windturbine.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19881240 Mathematical Model for Progressive Phase Distribution of Ku-band Reflectarray Antennas
Authors: M. Y. Ismail, M. Inam, A. F. M. Zain, N. Misran
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Progressive phase distribution is an important consideration in reflectarray antenna design which is required to form a planar wave in front of the reflectarray aperture. This paper presents a detailed mathematical model in order to determine the required reflection phase values from individual element of a reflectarray designed in Ku-band frequency range. The proposed technique of obtaining reflection phase can be applied for any geometrical design of elements and is independent of number of array elements. Moreover the model also deals with the solution of reflectarray antenna design with both centre and off-set feed configurations. The theoretical modeling has also been implemented for reflectarrays constructed on 0.508mm thickness of different dielectric substrates. The results show an increase in the slope of the phase curve from 4.61°/mm to 22.35°/mm by varying the material properties.
Keywords: Mathematical modeling, Progressive phase distribution, Reflectarray antenna, Reflection phase.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20671239 Optimal Design of UPFC Based Damping Controller Using Iteration PSO
Authors: Amin Safari, Hossein Shayeghi
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This paper presents a novel approach for tuning unified power flow controller (UPFC) based damping controller in order to enhance the damping of power system low frequency oscillations. The design problem of damping controller is formulated as an optimization problem according to the eigenvalue-based objective function which is solved using iteration particle swarm optimization (IPSO). The effectiveness of the proposed controller is demonstrated through eigenvalue analysis and nonlinear time-domain simulation studies under a wide range of loading conditions. The simulation study shows that the designed controller by IPSO performs better than CPSO in finding the solution. Moreover, the system performance analysis under different operating conditions show that the δE based controller is superior to the mB based controller.
Keywords: UPFC, Optimization Problem, Iteration ParticleSwarm Optimization, Damping Controller, Low FrequencyOscillations.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18041238 Investigation on Performance of Change Point Algorithm in Time Series Dynamical Regimes and Effect of Data Characteristics
Authors: Farhad Asadi, Mohammad Javad Mollakazemi
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In this paper, Bayesian online inference in models of data series are constructed by change-points algorithm, which separated the observed time series into independent series and study the change and variation of the regime of the data with related statistical characteristics. variation of statistical characteristics of time series data often represent separated phenomena in the some dynamical system, like a change in state of brain dynamical reflected in EEG signal data measurement or a change in important regime of data in many dynamical system. In this paper, prediction algorithm for studying change point location in some time series data is simulated. It is verified that pattern of proposed distribution of data has important factor on simpler and smother fluctuation of hazard rate parameter and also for better identification of change point locations. Finally, the conditions of how the time series distribution effect on factors in this approach are explained and validated with different time series databases for some dynamical system.
Keywords: Time series, fluctuation in statistical characteristics, optimal learning.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18121237 Evaluation Rabbit Serum of the Immunodominant Proteins of Mycobacterium Avium Paratuberculosis Extracts
Authors: M. Hashemi, R. Madani, N. Razmi
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M. paratuberculosis is a slow growing mycobactin dependent mycobacterial species known to be the causative agent of Johne’s disease in all species of domestic ruminants worldwide. JD is characterized by gradual weight loss; decreased milk production. Excretion of the organism may occur for prolonged periods (1 to 2.5 years) before the onset of clinical disease. In recent years researchers focus on identification a specific antigen of MAP to use in diagnosis test and preparation of effective vaccine. In this paper, for production of polyclonal antibody against proteins of Mycobacterium avium paratuberculosis cell well a rabbit immunization at a certain time period with antigen. After immunization of the animal, rabbit was bleeded for producing enriched serum. Antibodies were purification with ion exchange chromatography. For exact measurement of interaction, western blotting test was used that this study demonstrated sharp bands appears in nitrocellulose paper and specific bands were 50 and 150 KD molecular weight. These were indicating immunodominant proteins.
Keywords: Paratuberculosis, Immunodominant, Western blotting, Ion exchange choromatography.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 17571236 Dynamic Amplification Factors of Some City Bridges
Authors: I. Paeglite, A. Paeglitis
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Paper presents a study about dynamic effects obtained from the dynamic load testing of the city highway bridges in Latvia carried out from 2005 to 2012. 9 prestressed concrete bridges and 4 composite bridges were considered. 11 of 13 bridges were designed according to the Eurocodes but two according to the previous structural codes used in Latvia (SNIP 2.05.03-84). The dynamic properties of the bridges were obtained by heavy vehicle passing the bridge roadway with different driving speeds and with or without even pavement. The obtained values of the Dynamic amplification factor (DAF) and the bridge natural frequency were analyzed and compared to the values of built-in traffic load models provided in Eurocode 1. The actual DAF values for even bridge pavement in the most cases are smaller than the value adopted in Eurocode 1. Vehicle speed for uneven pavements significantly influence Dynamic amplification factor values.
Keywords: Bridge, dynamic effects, load testing, dynamic amplification factor.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 30001235 A Digital Twin Approach for Sustainable Territories Planning: A Case Study on District Heating
Authors: A. Amrani, O. Allali, A. Ben Hamida, F. Defrance, S. Morland, E. Pineau, T. Lacroix
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The energy planning process is a very complex task that involves several stakeholders and requires the consideration of several local and global factors and constraints. In order to optimize and simplify this process, we propose a tool-based iterative approach applied to district heating planning. We build our tool with the collaboration of a French territory using actual district data and implementing the European incentives. We set up an iterative process including data visualization and analysis, identification and extraction of information related to the area concerned by the operation, design of sustainable planning scenarios leveraging local renewable and recoverable energy sources, and finally, the evaluation of scenarios. The last step is performed by a dynamic digital twin replica of the city. Territory’s energy experts confirm that the tool provides them with valuable support towards sustainable energy planning.
Keywords: Climate change, data management, decision support, digital twin, district heating, energy planning, renewables, smart city.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6541234 Manufacturing of Full Automatic Carwash Using with Intelligent Control Algorithms
Authors: Amir Hossein Daei Sorkhabi, Bita Khazini
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In this paper the intelligent control of full automatic car wash using a programmable logic controller (PLC) has been investigated and designed to do all steps of carwashing. The Intelligent control of full automatic carwash has the ability to identify and profile the geometrical dimensions of the vehicle chassis. Vehicle dimension identification is an important point in this control system to adjust the washing brushes position and time duration. The study also tries to design a control set for simulating and building the automatic carwash. The main purpose of the simulation is to develop criteria for designing and building this type of carwash in actual size to overcome challenges of automation. The results of this research indicate that the proposed method in process control not only increases productivity, speed, accuracy and safety but also reduce the time and cost of washing based on dynamic model of the vehicle. A laboratory prototype based on an advanced intelligent control has been built to study the validity of the design and simulation which it’s appropriate performance confirms the validity of this study.
Keywords: Automatic Carwash, Dimension, PLC.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 68241233 Multiple Moving Talker Tracking by Integration of Two Successive Algorithms
Authors: Kenji Suyama, Masahiro Oshida, Noboru Owada
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In this paper, an estimation accuracy of multiple moving talker tracking using a microphone array is improved. The tracking can be achieved by the adaptive method in which two algorithms are integrated, namely, the PAST (Projection Approximation Subspace Tracking) algorithm and the IPLS (Interior Point Least Square) algorithm. When either talker begins to speak again after a silent period, an appropriate feasible region for an evaluation function of the IPLS algorithm might not be set. Then, the tracking fails due to the incorrect updating. Therefore, if an increment of the number of active talkers is detected, the feasible region must be reset. Then, a low cost realization is required for the high speed tracking and a high accuracy realization is desired for the precise tracking. In this paper, the directions roughly estimated using the delayed-sum-array method are used for the resetting. Several results of experiments performed in an actual room environment show the effectiveness of the proposed method.Keywords: moving talkers tracking, microphone array, signal subspace
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 13371232 Robustness of Hybrid Learning Acceleration Feedback Control Scheme in Flexible Manipulators
Authors: M. Z Md Zain, M. O. Tokhi, M. S. Alam
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This paper describes a practical approach to design and develop a hybrid learning with acceleration feedback control (HLC) scheme for input tracking and end-point vibration suppression of flexible manipulator systems. Initially, a collocated proportionalderivative (PD) control scheme using hub-angle and hub-velocity feedback is developed for control of rigid-body motion of the system. This is then extended to incorporate a further hybrid control scheme of the collocated PD control and iterative learning control with acceleration feedback using genetic algorithms (GAs) to optimize the learning parameters. Experimental results of the response of the manipulator with the control schemes are presented in the time and frequency domains. The performance of the HLC is assessed in terms of input tracking, level of vibration reduction at resonance modes and robustness with various payloads.Keywords: Flexible manipulator, iterative learning control, vibration suppression.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18211231 Site-Specific Approach for Seismic Design Spectra in Iran, Based On Recent Major Strong Ground Motions
Authors: Danesh Nourzadeh, Majid Ebad-Sichani, Shiro Takada
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Widespread use of response spectra in seismic design and evaluation of different types of structures makes them one of the most important seismic inputs. This importance urges the local design codes to adapt precise data based on updated information about the recent major earthquakes happened and also localized geotechnical data. In this regard, this paper derives the response spectra with a geotechnical approach for various scenarios coming from the recent major earthquakes happened in Iran for different types of hard soils, and compares the results to the corresponding spectra from the current seismic code. This comparison implies the need for adapting new design spectra for seismic design, because of major differences in the frequency domains and amplifications.
Keywords: Earthquake engineering, response spectra, seismic design, site response.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 24321230 Nonlinear Dynamics of Cracked RC Beams under Harmonic Excitation
Authors: Atul Krishna Banik
Abstract:
Nonlinear response behaviour of a cracked RC beam under harmonic excitation is analysed to investigate various instability phenomena like, bifurcation, jump phenomena etc. The nonlinearity of the system arises due to opening and closing of the cracks in the RC beam and is modelled as a cubic polynomial. In order to trace different branches at the bifurcation point on the response curve (amplitude versus frequency of excitation plot), an arc length continuation technique along with the incremental harmonic balance (IHBC) method is employed. The stability of the solution is investigated by the Floquet theory using Hsu-s scheme. The periodic solutions obtained by the IHBC method are compared with these obtained by the numerical integration of the equation of motion. Characteristics of solutions fold bifurcation, jump phenomena and from stable to unstable zones are identified.
Keywords: Incremental harmonic balance, arc-length continuation, bifurcation, jump phenomena.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15221229 Coverage and Connectivity Problem in Sensor Networks
Authors: Meenakshi Bansal, Iqbal Singh, Parvinder S. Sandhu
Abstract:
In over deployed sensor networks, one approach to Conserve energy is to keep only a small subset of sensors active at Any instant. For the coverage problems, the monitoring area in a set of points that require sensing, called demand points, and consider that the node coverage area is a circle of range R, where R is the sensing range, If the Distance between a demand point and a sensor node is less than R, the node is able to cover this point. We consider a wireless sensor network consisting of a set of sensors deployed randomly. A point in the monitored area is covered if it is within the sensing range of a sensor. In some applications, when the network is sufficiently dense, area coverage can be approximated by guaranteeing point coverage. In this case, all the points of wireless devices could be used to represent the whole area, and the working sensors are supposed to cover all the sensors. We also introduce Hybrid Algorithm and challenges related to coverage in sensor networks.Keywords: Wireless sensor networks, network coverage, Energy conservation, Hybrid Algorithms.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1723