Search results for: image guided radiation therapy
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 2239

Search results for: image guided radiation therapy

2179 Labeling Method in Steganography

Authors: H. Motameni, M. Norouzi, M. Jahandar, A. Hatami

Abstract:

In this paper a way of hiding text message (Steganography) in the gray image has been presented. In this method tried to find binary value of each character of text message and then in the next stage, tried to find dark places of gray image (black) by converting the original image to binary image for labeling each object of image by considering on 8 connectivity. Then these images have been converted to RGB image in order to find dark places. Because in this way each sequence of gray color turns into RGB color and dark level of grey image is found by this way if the Gary image is very light the histogram must be changed manually to find just dark places. In the final stage each 8 pixels of dark places has been considered as a byte and binary value of each character has been put in low bit of each byte that was created manually by dark places pixels for increasing security of the main way of steganography (LSB).

Keywords: Binary image, labeling, low bit, neighborhood, RGB image, steganography, threshold.

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2178 Image Segmentation and Contour Recognition Based on Mathematical Morphology

Authors: Pinaki Pratim Acharjya, Esha Dutta

Abstract:

In image segmentation contour detection is one of the important pre-processing steps in recent days. Contours characterize boundaries and contour detection is one of the most difficult tasks in image processing. Hence it is a problem of fundamental importance in image processing. Contour detection of an image decreases the volume of data considerably and useless information is removed, but the structural properties of the image remain same. In this research, a robust and effective contour detection technique has been proposed using mathematical morphology. Three different contour detection results are obtained by using morphological dilation and erosion. The comparative analyses of three different results also have been done.

Keywords: Image segmentation, contour detection, mathematical morphology.

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2177 Parallel Image Compression and Analysis with Wavelets

Authors: M. Kutila, J. Viitanen

Abstract:

This paper presents image compression with wavelet based method. The wavelet transformation divides image to low- and high pass filtered parts. The traditional JPEG compression technique requires lower computation power with feasible losses, when only compression is needed. However, there is obvious need for wavelet based methods in certain circumstances. The methods are intended to the applications in which the image analyzing is done parallel with compression. Furthermore, high frequency bands can be used to detect changes or edges. Wavelets enable hierarchical analysis for low pass filtered sub-images. The first analysis can be done for a small image, and only if any interesting is found, the whole image is processed or reconstructed.

Keywords: image compression, jpeg, wavelet, vlc

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2176 An Image Matching Method for Digital Images Using Morphological Approach

Authors: Pinaki Pratim Acharjya, Dibyendu Ghoshal

Abstract:

Image matching methods play a key role in deciding correspondence between two image scenes. This paper presents a method for the matching of digital images using mathematical morphology. The proposed method has been applied to real life images. The matching process has shown successful and promising results.

Keywords: Digital image, gradients, image matching, mathematical morphology.

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2175 Ellagic Acid Enhanced Apoptotic Radiosensitivity via G1 Cell Cycle Arrest and γ-H2AX Foci Formation in HeLa Cells in vitro

Authors: V. R. Ahire, A. Kumar, B. N. Pandey, K. P. Mishra, G. R. Kulkarni

Abstract:

Radiation therapy is an effective vital strategy used globally in the treatment of cervical cancer. However, radiation efficacy principally depends on the radiosensitivity of the tumor, and not all patient exhibit significant response to irradiation. A radiosensitive tumor is easier to cure than a radioresistant tumor which later advances to local recurrence and metastasis. Herbal polyphenols are gaining attention for exhibiting radiosensitization through various signaling. Current work focuses to study the radiosensitization effect of ellagic acid (EA), on HeLa cells. EA intermediated radiosensitization of HeLa cells was due to the induction γ-H2AX foci formation, G1 phase cell cycle arrest, and loss of reproductive potential, growth inhibition, drop in the mitochondrial membrane potential and protein expression studies that eventually induced apoptosis. Irradiation of HeLa in presence of EA (10 μM) to doses of 2 and 4 Gy γ-radiation produced marked tumor cytotoxicity. EA also demonstrated radio-protective effect on normal cell, NIH3T3 and aided recovery from the radiation damage. Our results advocate EA to be an effective adjuvant for improving cancer radiotherapy as it displays striking tumor cytotoxicity and reduced normal cell damage instigated by irradiation.

Keywords: Apoptotic radiosensitivity, ellagic acid, mitochondrial potential, cell-cycle arrest.

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2174 Integral Image-Based Differential Filters

Authors: Kohei Inoue, Kenji Hara, Kiichi Urahama

Abstract:

We describe a relationship between integral images and differential images. First, we derive a simple difference filter from conventional integral image. In the derivation, we show that an integral image and the corresponding differential image are related to each other by simultaneous linear equations, where the numbers of unknowns and equations are the same, and therefore, we can execute the integration and differentiation by solving the simultaneous equations. We applied the relationship to an image fusion problem, and experimentally verified the effectiveness of the proposed method.

Keywords: Integral images, differential images, differential filters, image fusion.

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2173 Identifying the Best Global Solar Radiation Model for Hutat Suder, Saudi Arabia

Authors: H. Al-Sholigom, Z. Al-Mostafa

Abstract:

Many associations and experimental models have been developed to estimate solar radiation around the world. The duration of sunshine is the most commonly used parameter for estimating global solar radiation because it can be easily and reliably measured. To estimate the global monthly solar average on horizontal surfaces, we used 52 models with widely available data in Hutat Suder, Saudi Arabia. After testing the models, some were not suitable for use in this area, while others differed in performance. The best models have been identified.

Keywords: Earth, Global solar radiation, Hutat Suder, Saudi Arabia, sunshine, measured data.

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2172 Evaluating Content Based Image Retrieval Techniques with the One Million Images CLIC Test Bed

Authors: Pierre-Alain Moëllic, Patrick Hède, Gr egory Grefenstette, Christophe Millet

Abstract:

Pattern recognition and image recognition methods are commonly developed and tested using testbeds, which contain known responses to a query set. Until now, testbeds available for image analysis and content-based image retrieval (CBIR) have been scarce and small-scale. Here we present the one million images CEA-List Image Collection (CLIC) testbed that we have produced, and report on our use of this testbed to evaluate image analysis merging techniques. This testbed will soon be made publicly available through the EU MUSCLE Network of Excellence.

Keywords: CBIR, CLIC, evaluation, image indexing and retrieval, testbed.

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2171 A CT-based Monte Carlo Dose Calculations for Proton Therapy Using a New Interface Program

Authors: A. Esmaili Torshabi, A. Terakawa, K. Ishii, H. Yamazaki, S. Matsuyama, Y. Kikuchi, M. Nakhostin, H. Sabet, A. Ishizaki, W. Yamashita, T. Togashi, J. Arikawa, H. Akiyama, K. Koyata

Abstract:

The purpose of this study is to introduce a new interface program to calculate a dose distribution with Monte Carlo method in complex heterogeneous systems such as organs or tissues in proton therapy. This interface program was developed under MATLAB software and includes a friendly graphical user interface with several tools such as image properties adjustment or results display. Quadtree decomposition technique was used as an image segmentation algorithm to create optimum geometries from Computed Tomography (CT) images for dose calculations of proton beam. The result of the mentioned technique is a number of nonoverlapped squares with different sizes in every image. By this way the resolution of image segmentation is high enough in and near heterogeneous areas to preserve the precision of dose calculations and is low enough in homogeneous areas to reduce the number of cells directly. Furthermore a cell reduction algorithm can be used to combine neighboring cells with the same material. The validation of this method has been done in two ways; first, in comparison with experimental data obtained with 80 MeV proton beam in Cyclotron and Radioisotope Center (CYRIC) in Tohoku University and second, in comparison with data based on polybinary tissue calibration method, performed in CYRIC. These results are presented in this paper. This program can read the output file of Monte Carlo code while region of interest is selected manually, and give a plot of dose distribution of proton beam superimposed onto the CT images.

Keywords: Monte Carlo, CT images, Quadtree decomposition, Interface program, Proton beam

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2170 Workplace Monitoring During Interventional Cardiology Procedures

Authors: N. Todorovic, I. Bikit, J. Nikolov, S. Forkapic, D. Mrdja, S. Todorovic

Abstract:

Interventional cardiologists are at greater risk from radiation exposure as a result of the procedures they undertake than most other medical specialists. A study was performed to evaluate operator dose during interventional cardiology procedures and to establish methods of operator dose reduction with a radiation protective device. Different procedure technique and use of protective tools can explain big difference in the annual equivalent dose received by the professionals. Strategies to prevent and monitor radiation exposure, advanced protective shielding and effective radiation monitoring methods should be applied.

Keywords: absorbed dose rate measurements, annualequivalent dose, protective device.

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2169 Modeling and Numerical Simulation of Sound Radiation by the Boundary Element Method

Authors: Costa, E.S., Borges, E.N.M., Afonso, M.M.

Abstract:

The modeling of sound radiation is of fundamental importance for understanding the propagation of acoustic waves and, consequently, develop mechanisms for reducing acoustic noise. The propagation of acoustic waves, are involved in various phenomena such as radiation, absorption, transmission and reflection. The radiation is studied through the linear equation of the acoustic wave that is obtained through the equation for the Conservation of Momentum, equation of State and Continuity. From these equations, is the Helmholtz differential equation that describes the problem of acoustic radiation. In this paper we obtained the solution of the Helmholtz differential equation for an infinite cylinder in a pulsating through free and homogeneous. The analytical solution is implemented and the results are compared with the literature. A numerical formulation for this problem is obtained using the Boundary Element Method (BEM). This method has great power for solving certain acoustical problems in open field, compared to differential methods. BEM reduces the size of the problem, thereby simplifying the input data to be worked and reducing the computational time used.

Keywords: Acoustic radiation, boundary element

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2168 The Comparison of Finite Difference Methods for Radiation Diffusion Equations

Authors: Ren Jian, Yang Shulin

Abstract:

In this paper, the difference between the Alternating Direction Method (ADM) and the Non-Splitting Method (NSM) is investigated, while both methods applied to the simulations for 2-D multimaterial radiation diffusion issues. Although the ADM have the same accuracy orders with the NSM on the uniform meshes, the accuracy of ADM will decrease on the distorted meshes or the boundary of domain. Numerical experiments are carried out to confirm the theoretical predication.

Keywords: Alternating Direction Method, Non-SplittingMethod, Radiation Diffusion.

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2167 Efficient CT Image Volume Rendering for Diagnosis

Authors: HaeNa Lee, Sun K. Yoo

Abstract:

Volume rendering is widely used in medical CT image visualization. Applying 3D image visualization to diagnosis application can require accurate volume rendering with high resolution. Interpolation is important in medical image processing applications such as image compression or volume resampling. However, it can distort the original image data because of edge blurring or blocking effects when image enhancement procedures were applied. In this paper, we proposed adaptive tension control method exploiting gradient information to achieve high resolution medical image enhancement in volume visualization, where restored images are similar to original images as much as possible. The experimental results show that the proposed method can improve image quality associated with the adaptive tension control efficacy.

Keywords: Tension control, Interpolation, Ray-casting, Medical imaging analysis.

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2166 Dispersed Error Control based on Error Filter Design for Improving Halftone Image Quality

Authors: Sang-Chul Kim, Sung-Il Chien

Abstract:

The error diffusion method generates worm artifacts, and weakens the edge of the halftone image when the continuous gray scale image is reproduced by a binary image. First, to enhance the edges, we propose the edge-enhancing filter by considering the quantization error information and gradient of the neighboring pixels. Furthermore, to remove worm artifacts often appearing in a halftone image, we add adaptively random noise into the weights of an error filter.

Keywords: Artifact suppression, Edge enhancement, Error diffusion method, Halftone image

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2165 Evolution of Cord Absorbed Dose during of Larynx Cancer Radiotherapy, with 3D Treatment Planning and Tissue Equivalent Phantom

Authors: Mohammad Hassan Heidari, Amir Hossein Goodarzi, Majid Azarniush

Abstract:

Radiation doses to tissues and organs were measured using the anthropomorphic phantom as an equivalent to the human body. When high-energy X-rays are externally applied to treat laryngeal cancer, the absorbed dose at the laryngeal lumen is lower than given dose because of air space, which it should pass through, before reaching the lesion. Specially, in case of high-energy X-rays, the loss of dose is considerable. Three-dimensional absorbed dose distributions have been computed for high-energy photon radiation therapy of laryngeal and hypopharyngeal cancers, using a coaxial pair of opposing lateral beams in fixed positions. Treatment plans obtained under various conditions of irradiation.

Keywords: 3D Treatment Planning, anthropomorphic phantom, larynx cancer, radiotherapy.

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2164 Simulation of Hamming Coding and Decoding for Microcontroller Radiation Hardening

Authors: Rehab I. Abdul Rahman, Mazhar B. Tayel

Abstract:

This paper presents a method of hardening the 8051 micro-controller, able to assure reliable operation in the presence of bit flips caused by radiation. Aiming at avoiding such faults in the 8051 micro-controller, Hamming code protection was used in its SRAM memory and registers. A VHDL code has been used for this hamming code protection.

Keywords: Radiation, hardening, bitflip, hamming code.

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2163 Radiation Effect on Unsteady MHD Flow over a Stretching Surface

Authors: Zanariah Mohd Yusof, Siti Khuzaimah Soid, Ahmad Sukri Abd Aziz, Seripah Awang Kechil

Abstract:

Unsteady magnetohydrodynamics (MHD) boundary layer flow and heat transfer over a continuously stretching surface in the presence of radiation is examined. By similarity transformation, the governing partial differential equations are transformed to a set of ordinary differential equations. Numerical solutions are obtained by employing the Runge-Kutta-Fehlberg method scheme with shooting technique in Maple software environment. The effects of unsteadiness parameter, radiation parameter, magnetic parameter and Prandtl number on the heat transfer characteristics are obtained and discussed. It is found that the heat transfer rate at the surface increases as the Prandtl number and unsteadiness parameter increase but decreases with magnetic and radiation parameter.

Keywords: Heat transfer, magnetohydrodynamics, radiation, unsteadiness.

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2162 Ribbon Beam Antenna for RFID Technology

Authors: T. Zalabsky, P. Bezousek, T. Shejbal

Abstract:

The paper describes new concept of the ribbon beam antenna for RFID technology. Antenna is located near to railway lines to monitor tags situated on trains. Antenna works at 2.45 GHz and it is fabricated by microstrip technology. Antenna contains two same mirrored parts having the same radiation patterns. Each part consists of three dielectric layers. The first layer has on one side radiation elements. The second layer is only for mechanical construction and it sets optimal electromagnetic field for each radiating elements. The third layer has on its top side a ground plane and on the bottom side a microstrip circuit used for individual radiation elements feeding.

Keywords: RFID, cosecant radiation pattern, ribbon beam, patch antenna, microstrip.

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2161 A Novel Approach to Image Compression of Colour Images by Plane Reduction Technique

Authors: K.Sowmyan, A.Siddarth, D.Menaka

Abstract:

Several methods have been proposed for color image compression but the reconstructed image had very low signal to noise ratio which made it inefficient. This paper describes a lossy compression technique for color images which overcomes the drawbacks. The technique works on spatial domain where the pixel values of RGB planes of the input color image is mapped onto two dimensional planes. The proposed technique produced better results than JPEG2000, 2DPCA and a comparative study is reported based on the image quality measures such as PSNR and MSE.Experiments on real time images are shown that compare this methodology with previous ones and demonstrate its advantages.

Keywords: Color Image compression, spatial domain, planereduction, root mean square, image restoration

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2160 A Trends Analysis of Image Processing in Unmanned Aerial Vehicle

Authors: Jae-Neung Lee, Keun-Chang Kwak

Abstract:

This paper describes an analysis of domestic and international trends of image processing for data in UAV (unmanned aerial vehicle) and also explains about UAV and Quadcopter. Overseas examples of image processing using UAV include image processing for totaling the total numberof vehicles, edge/target detection, detection and evasion algorithm, image processing using SIFT(scale invariant features transform) matching, and application of median filter and thresholding. In Korea, many studies are underway including visualization of new urban buildings.

Keywords: Image Processing, UAV, Quadcopter, Target detection.

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2159 Proprioceptive Neuromuscular Facilitation Exercises of Upper Extremities Assessment Using Microsoft Kinect Sensor and Color Marker in a Virtual Reality Environment

Authors: M. Owlia, M. H. Azarsa, M. Khabbazan, A. Mirbagheri

Abstract:

Proprioceptive neuromuscular facilitation exercises are a series of stretching techniques that are commonly used in rehabilitation and exercise therapy. Assessment of these exercises for true maneuvering requires extensive experience in this field and could not be down with patients themselves. In this paper, we developed software that uses Microsoft Kinect sensor, a spherical color marker, and real-time image processing methods to evaluate patient’s performance in generating true patterns of movements. The software also provides the patient with a visual feedback by showing his/her avatar in a Virtual Reality environment along with the correct path of moving hand, wrist and marker. Primary results during PNF exercise therapy of a patient in a room environment shows the ability of the system to identify any deviation of maneuvering path and direction of the hand from the one that has been performed by an expert physician.

Keywords: Image processing, Microsoft Kinect, proprioceptive neuromuscular facilitation, upper extremities assessment, virtual reality.

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2158 Robust Semi-Blind Digital Image Watermarking Technique in DT-CWT Domain

Authors: Samira Mabtoul, Elhassan Ibn Elhaj, Driss Aboutajdine

Abstract:

In this paper a new robust digital image watermarking algorithm based on the Complex Wavelet Transform is proposed. This technique embeds different parts of a watermark into different blocks of an image under the complex wavelet domain. To increase security of the method, two chaotic maps are employed, one map is used to determine the blocks of the host image for watermark embedding, and another map is used to encrypt the watermark image. Simulation results are presented to demonstrate the effectiveness of the proposed algorithm.

Keywords: Image watermarking, Chaotic map, DT-CWT.

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2157 Fuzzy Hyperbolization Image Enhancement and Artificial Neural Network for Anomaly Detection

Authors: Sri Hartati, 1Agus Harjoko, Brad G. Nickerson

Abstract:

A prototype of an anomaly detection system was developed to automate process of recognizing an anomaly of roentgen image by utilizing fuzzy histogram hyperbolization image enhancement and back propagation artificial neural network. The system consists of image acquisition, pre-processor, feature extractor, response selector and output. Fuzzy Histogram Hyperbolization is chosen to improve the quality of the roentgen image. The fuzzy histogram hyperbolization steps consist of fuzzyfication, modification of values of membership functions and defuzzyfication. Image features are extracted after the the quality of the image is improved. The extracted image features are input to the artificial neural network for detecting anomaly. The number of nodes in the proposed ANN layers was made small. Experimental results indicate that the fuzzy histogram hyperbolization method can be used to improve the quality of the image. The system is capable to detect the anomaly in the roentgen image.

Keywords: Image processing, artificial neural network, anomaly detection.

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2156 Radiation Dose Distribution for Workers in South Korean Nuclear Power Plants

Authors: B. I. Lee, S. I. Kim, D. H. Suh, J. I. Kim, Y. K. Lim

Abstract:

A total of 33,680 nuclear power plants (NPPs) workers were monitored and recorded from 1990 to 2007. According to the record, the average individual radiation dose has been decreasing continually from it 3.20 mSv/man in 1990 to 1.12 mSv/man at the end of 2007. After the International Commission on Radiological Protection (ICRP) 60 recommendation was generalized in South Korea, no nuclear power plant workers received above 20 mSv radiation, and the numbers of relatively highly exposed workers have been decreasing continuously. The age distribution of radiation workers in nuclear power plants was composed of mainly 20-30- year-olds (83%) for 1990 ~ 1994 and 30-40-year-olds (75%) for 2003 ~ 2007. The difference in individual average dose by age was not significant. Most (77%) of NPP radiation exposures from 1990 to 2007 occurred mostly during the refueling period. With regard to exposure type, the majority of exposures were external exposures, representing 95% of the total exposures, while internal exposures represented only 5%. External effective dose was affected mainly by gamma radiation exposure, with an insignificant amount of neutron exposure. As for internal effective dose, tritium (3H) in the pressurized heavy water reactor (PHWR) was the biggest cause of exposure.

Keywords: Dose distribution, External exposure, Nuclear powerplant, Occupational radiation dose

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2155 Mixed Convection with Radiation Effect over a Nonlinearly Stretching Sheet

Authors: Kai-Long Hsiao

Abstract:

In this study, an analysis has been performed for free convection with radiation effect over a thermal forming nonlinearly stretching sheet. Parameters n, k0, Pr, G represent the dominance of the nonlinearly effect, radiation effect, heat transfer and free convection effects which have been presented in governing equations, respectively. The similarity transformation and the finite-difference methods have been used to analyze the present problem. From the results, we find that the effects of parameters n, k0, Pr, Ec and G to the nonlinearly stretching sheet. The increase of Prandtl number Pr, free convection parameter G or radiation parameter k0 resulting in the increase of heat transfer effects, but increase of the viscous dissipation number Ec will decrease of heat transfer effect.

Keywords: Nonlinearly stretching sheet, Free convection, Finite-difference, Radiation effect.

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2154 On the Reduction of Side Effects in Tomography

Authors: V. Masilamani, C. Vanniarajan, Kamala Krithivasan

Abstract:

As the Computed Tomography(CT) requires normally hundreds of projections to reconstruct the image, patients are exposed to more X-ray energy, which may cause side effects such as cancer. Even when the variability of the particles in the object is very less, Computed Tomography requires many projections for good quality reconstruction. In this paper, less variability of the particles in an object has been exploited to obtain good quality reconstruction. Though the reconstructed image and the original image have same projections, in general, they need not be the same. In addition to projections, if a priori information about the image is known, it is possible to obtain good quality reconstructed image. In this paper, it has been shown by experimental results why conventional algorithms fail to reconstruct from a few projections, and an efficient polynomial time algorithm has been given to reconstruct a bi-level image from its projections along row and column, and a known sub image of unknown image with smoothness constraints by reducing the reconstruction problem to integral max flow problem. This paper also discusses the necessary and sufficient conditions for uniqueness and extension of 2D-bi-level image reconstruction to 3D-bi-level image reconstruction.

Keywords: Discrete Tomography, Image Reconstruction, Projection, Computed Tomography, Integral Max Flow Problem, Smooth Binary Image.

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2153 Salient Points Reduction for Content-Based Image Retrieval

Authors: Yao-Hong Tsai

Abstract:

Salient points are frequently used to represent local properties of the image in content-based image retrieval. In this paper, we present a reduction algorithm that extracts the local most salient points such that they not only give a satisfying representation of an image, but also make the image retrieval process efficiently. This algorithm recursively reduces the continuous point set by their corresponding saliency values under a top-down approach. The resulting salient points are evaluated with an image retrieval system using Hausdoff distance. In this experiment, it shows that our method is robust and the extracted salient points provide better retrieval performance comparing with other point detectors.

Keywords: Barnard detector, Content-based image retrieval, Points reduction, Salient point.

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2152 Development of EPID-based Real time Dose Verification for Dynamic IMRT

Authors: Todsaporn Fuangrod, Daryl J. O'Connor, Boyd MC McCurdy, Peter B. Greer

Abstract:

An electronic portal image device (EPID) has become a method of patient-specific IMRT dose verification for radiotherapy. Research studies have focused on pre and post-treatment verification, however, there are currently no interventional procedures using EPID dosimetry that measure the dose in real time as a mechanism to ensure that overdoses do not occur and underdoses are detected as soon as is practically possible. As a result, an EPID-based real time dose verification system for dynamic IMRT was developed and was implemented with MATLAB/Simulink. The EPID image acquisition was set to continuous acquisition mode at 1.4 images per second. The system defined the time constraint gap, or execution gap at the image acquisition time, so that every calculation must be completed before the next image capture is completed. In addition, the <=-evaluation method was used for dose comparison, with two types of comparison processes; individual image and cumulative dose comparison monitored. The outputs of the system are the <=-map, the percent of <=<1, and mean-<= versus time, all in real time. Two strategies were used to test the system, including an error detection test and a clinical data test. The system can monitor the actual dose delivery compared with the treatment plan data or previous treatment dose delivery that means a radiation therapist is able to switch off the machine when the error is detected.

Keywords: real-time dose verification, EPID dosimetry, simulation, dynamic IMRT

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2151 Blur and Ringing Artifact Measurement in Image Compression using Wavelet Transform

Authors: Madhuri Khambete, Madhuri Joshi

Abstract:

Quality evaluation of an image is an important task in image processing applications. In case of image compression, quality of decompressed image is also the criterion for evaluation of given coding scheme. In the process of compression -decompression various artifacts such as blocking artifacts, blur artifact, ringing or edge artifact are observed. However quantification of these artifacts is a difficult task. We propose here novel method to quantify blur and ringing artifact in an image.

Keywords: Blur, Compression, Objective Quality assessment, Ringing artifact.

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2150 In vitro Study of Laser Diode Radiation Effect on the Photo-Damage of MCF-7 and MCF-10A Cell Clusters

Authors: A. Dashti, M. Eskandari, L. Farahmand, P. Parvin, A. Jafargholi

Abstract:

Breast Cancer is one of the most considerable diseases in the United States and other countries and is the second leading cause of death in women. Common breast cancer treatments would lead to adverse side effects such as loss of hair, nausea, and weakness. These complications arise because these cancer treatments damage some healthy cells while eliminating the cancer cells. In an effort to address these complications, laser radiation was utilized and tested as a targeted cancer treatment for breast cancer. In this regard, tissue engineering approaches are being employed by using an electrospun scaffold in order to facilitate the growth of breast cancer cells. Polycaprolacton (PCL) was used as a material for scaffold fabricating because of its biocompatibility, biodegradability, and supporting cell growth. The specific breast cancer cells have the ability to create a three-dimensional cell cluster due to the spontaneous accumulation of cells in the porosity of the scaffold under some specific conditions. Therefore, we are looking for a higher density of porosity and larger pore size. Fibers showed uniform diameter distribution and final scaffold had optimum characteristics with approximately 40% porosity. The images were taken by SEM and the density and the size of the porosity were determined with the Image. After scaffold preparation, it has cross-linked by glutaraldehyde. Then, it has been washed with glycine and phosphate buffer saline (PBS), in order to neutralize the residual glutaraldehyde. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) results have represented approximately 91.13% viability of the scaffolds for cancer cells. In order to create a cluster, Michigan Cancer Foundation-7 (MCF-7, breast cancer cell line) and Michigan Cancer Foundation-10A (MCF-10A, human mammary epithelial cell line) cells were cultured on the scaffold in 24 well plate for five days. Then, we have exposed the cluster to the laser diode 808 nm radiation to investigate the effect of laser on the tumor with different power and time. Under the same conditions, cancer cells lost their viability more than the healthy ones. In conclusion, laser therapy is a viable method to destroy the target cells and has a minimum effect on the healthy tissues and cells and it can improve the other method of cancer treatments limitations.

Keywords: Breast cancer, electrospun scaffold, polycaprolacton, laser diode, cancer treatment.

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