Search results for: Approximate similarity

Authors: Amol Bapuso Rajmane, Pradeep M. Patil, Prakash J. Kulkarni

Abstract:

The Information Retrieval community is facing the problem of effective representation of Web search results. When we organize web search results into clusters it becomes easy to the users to quickly browse through search results. The traditional search engines organize search results into clusters for ambiguous queries, representing each cluster for each meaning of the query. The clusters are obtained according to the topical similarity of the retrieved search results, but it is possible for results to be totally dissimilar and still correspond to the same meaning of the query. People search is also one of the most common tasks on the Web nowadays, but when a particular person’s name is queried the search engines return web pages which are related to different persons who have the same queried name. By placing the burden on the user of disambiguating and collecting pages relevant to a particular person, in this paper, we have developed an approach that clusters web pages based on the association of the web pages to the different people and clusters that are based on generic entity search.

Keywords: Entity resolution, information retrieval, graph based disambiguation, web people search, clustering.

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Authors: M. Barański

Abstract:

This article presents a new vibration diagnostic method designed to (PM) machines with permanent magnets. Those devices are commonly used in small wind and water systems or vehicles drives. The author’s method is very innovative and unique. Specific structural properties of PM machines are used in this method - electromotive force (EMF) generated due to vibrations. There was analysed number of publications which describe vibration diagnostic methods and tests of electrical PM machines and there was no method found to determine the technical condition of such machine basing on their own signals. In this article will be discussed: the method genesis, the similarity of machines with permanent magnet to vibration sensor and simulation and laboratory tests results. The method of determination the technical condition of electrical machine with permanent magnets basing on its own signals is the subject of patent application and it is the main thesis of author’s doctoral dissertation.

Keywords: Electrical vehicle, generator, permanent magnet, traction drive, vibrations.

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Authors: Smriti H. Bhandari, S. M. Deshpande

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Surface metrology with image processing is a challenging task having wide applications in industry. Surface roughness can be evaluated using texture classification approach. Important aspect here is appropriate selection of features that characterize the surface. We propose an effective combination of features for multi-scale and multi-directional analysis of engineering surfaces. The features include standard deviation, kurtosis and the Canny edge detector. We apply the method by analyzing the surfaces with Discrete Wavelet Transform (DWT) and Dual-Tree Complex Wavelet Transform (DT-CWT). We used Canberra distance metric for similarity comparison between the surface classes. Our database includes the surface textures manufactured by three machining processes namely Milling, Casting and Shaping. The comparative study shows that DT-CWT outperforms DWT giving correct classification performance of 91.27% with Canberra distance metric.

Keywords: Dual-tree complex wavelet transform, surface metrology, surface roughness, texture classification.

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Authors: Cesar Hernández, Diego Giral, Ingrid Páez

Abstract:

This article proposes a hybrid algorithm for spectrum allocation in cognitive radio networks based on the algorithms Analytical Hierarchical Process (AHP) and Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) to improve the performance of the spectrum mobility of secondary users in cognitive radio networks. To calculate the level of performance of the proposed algorithm a comparative analysis between the proposed AHP-TOPSIS, Grey Relational Analysis (GRA) and Multiplicative Exponent Weighting (MEW) algorithm is performed. Four evaluation metrics are used. These metrics are accumulative average of failed handoffs, accumulative average of handoffs performed, accumulative average of transmission bandwidth, and accumulative average of the transmission delay. The results of the comparison show that AHP-TOPSIS Algorithm provides 2.4 times better performance compared to a GRA Algorithm and, 1.5 times better than the MEW Algorithm.

Keywords: Cognitive radio, decision making, hybrid algorithm, spectrum handoff, wireless networks.

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Authors: Mahmoud Elmezain, Ayoub Al-Hamadi, Robert Niese, Bernd Michaelis

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Real-time hand tracking is a challenging task in many computer vision applications such as gesture recognition. This paper proposes a robust method for hand tracking in a complex environment using Mean-shift analysis and Kalman filter in conjunction with 3D depth map. The depth information solve the overlapping problem between hands and face, which is obtained by passive stereo measuring based on cross correlation and the known calibration data of the cameras. Mean-shift analysis uses the gradient of Bhattacharyya coefficient as a similarity function to derive the candidate of the hand that is most similar to a given hand target model. And then, Kalman filter is used to estimate the position of the hand target. The results of hand tracking, tested on various video sequences, are robust to changes in shape as well as partial occlusion.

Keywords: Computer Vision and Image Analysis, Object Tracking, Gesture Recognition.

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Authors: A. Abou El-Azm Aly, A. Chong, F. Nicolleau, S. Beck

Abstract:

The fractal-shaped orifices are assumed to have a significant effect on the pressure drop downstream pipe flow due to their edge self-similarity shape which enhances the mixing properties. Here, we investigate the pressure drop after these fractals using a digital micro-manometer at different stations downstream a turbulent flow pipe then a direct comparison has been made with the pressure drop measured from regular orifices with the same flow area. Our results showed that the fractal-shaped orifices have a significant effect on the pressure drop downstream the flow. Also the pressure drop measured across the fractal-shaped orifices is noticed to be lower that that from ordinary orifices of the same flow areas. This result could be important in designing piping systems from point of view of losses consideration with the same flow control area. This is promising to use the fractal-shaped orifices as flowmeters as they can sense the pressure drop across them accurately with minimum losses than the regular ones.

Keywords: Fractal-shaped orifice, pressure drop, turbulent flow.

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Authors: Khalid Abd, Kazem Abhary, Romeo Marian

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Multiple criteria decision making (MCDM) is an approach to ranking the solutions and finding the best one when two or more solutions are provided. In this study, MCDM approach is proposed to select the most suitable scheduling rule of robotic flexible assembly cells (RFACs). Two MCDM approaches, Analytic Hierarchy Process (AHP) and Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) are proposed for solving the scheduling rule selection problem. The AHP method is employed to determine the weights of the evaluation criteria, while the TOPSIS method is employed to obtain final ranking order of scheduling rules. Four criteria are used to evaluate the scheduling rules. Also, four scheduling policies of RFAC are examined to choose the most appropriate one for this purpose. A numerical example illustrates applications of the suggested methodology. The results show that the methodology is practical and works in RFAC settings.

Keywords: AHP, TOPSIS, Scheduling rules selection

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Authors: Huda Algharib, Amal Algharib, Hanan Algharib, Ali Mohammad Alqudah

Abstract:

Image registration is an important topic for many imaging systems and computer vision applications. The standard image registration techniques such as Mutual information/ Normalized mutual information -based methods have a limited performance because they do not consider the spatial information or the relationships between the neighbouring pixels or voxels. In addition, the amount of image noise may significantly affect the registration accuracy. Therefore, this paper proposes an efficient method that explicitly considers the relationships between the adjacent pixels, where the gradient information of the reference and scene images is extracted first, and then the cosine similarity of the extracted gradient information is computed and used to improve the accuracy of the standard normalized mutual information measure. Our experimental results on different data types (i.e. CT, MRI and thermal images) show that the proposed method outperforms a number of image registration techniques in terms of the accuracy.

Keywords: Image registration, mutual information, image gradients, Image transformations.

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Authors: Dibyendu Ghoshal, Shimal Das

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Image compression based on fractal coding is a lossy compression method and normally used for gray level images range and domain blocks in rectangular shape. Fractal based digital image compression technique provide a large compression ratio and in this paper, it is proposed using YUV colour space and the fractal theory which is based on iterated transformation. Fractal geometry is mainly applied in the current study towards colour image compression coding. These colour images possesses correlations among the colour components and hence high compression ratio can be achieved by exploiting all these redundancies. The proposed method utilises the self-similarity in the colour image as well as the cross-correlations between them. Experimental results show that the greater compression ratio can be achieved with large domain blocks but more trade off in image quality is good to acceptable at less than 1 bit per pixel.

Keywords: Fractal coding, Iterated Function System (IFS), Image compression, YUV colour space.

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Authors: Pipat Lounglawan, Wassana Lounglawan, Wisitiporm Suksombat

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A study was conducted to determine the effect of feeding glycerol on dairy cows performance. Twenty four Holstein Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows in early lactation; averaging 13+2.4 kg of milk, 64+45 days in milk, 55+16 months old and 325+26 kg live weight, were stratified for milk yield, days in milk, age, stage of lactation and body weight, and then randomly allocated to three treatment groups. All cows were fed approximate 8 kg of concentrate together with ad libitum corn silage and freely access to clean water. Nil or 150 and 300g of glycerol were supplemented to the cows according to treatment groups. All cows consumed similar concentrate, corn silage and total DM and NELP. There were no significant differences in DM intake, CP intake, NELP intake, milk and milk composition yields. All cows had similar fat, protein, lactose, solid not fat and total solid percentage. All cows gain similar live weight. The present study indicated that, supplementation of glycerol did not enhance milk yield, milk composition and live weight change.

Keywords: Glycerol, Milk production and composition, Dairycattle

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Authors: F. Halek, A. Kavousi, M. Banifatemi

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There is growing interest in biodiesel (fatty acid methyl ester or FAME) because of the similarity in its properties when compared to those of diesel fuels. Diesel engines operated on biodiesel have lower emissions of carbon monoxide, unburned hydrocarbons, particulate matter, and air toxics than when operated on petroleum-based diesel fuel. Production of fatty acid methyl ester (FAME) from rapeseed (nonedible oil) fatty acid distillate having high free fatty acids (FFA) was investigated in this work. Conditions for esterification process of rapeseed oil were 1.8 % H2SO4 as catalyst, MeOH/oil of molar ratio 2 : 0.1 and reaction temperature 65 °C, for a period of 3h. The yield of methyl ester was > 90 % in 1 h. The amount of FFA was reduced from 93 wt % to less than 2 wt % at the end of the esterification process. The FAME was pureed by neutralization with 1 M sodium hydroxide in water solution at a reaction temperature of 62 °C. The final FAME product met with the biodiesel quality standard, and ASTM D 6751.

Keywords: Alternative Fuels, Biodiesel, Fatty Acid, MethylEster, Seed Oil, Transesterification.

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Authors: Ashish Thakur, Radhey Shyam Anand

Abstract:

This paper presents the region based segmentation method for ultrasound images using local statistics. In this segmentation approach the homogeneous regions depends on the image granularity features, where the interested structures with dimensions comparable to the speckle size are to be extracted. This method uses a look up table comprising of the local statistics of every pixel, which are consisting of the homogeneity and similarity bounds according to the kernel size. The shape and size of the growing regions depend on this look up table entries. The algorithms are implemented by using connected seeded region growing procedure where each pixel is taken as seed point. The region merging after the region growing also suppresses the high frequency artifacts. The updated merged regions produce the output in formed of segmented image. This algorithm produces the results that are less sensitive to the pixel location and it also allows a segmentation of the accurate homogeneous regions.

Keywords: Local statistics, region growing, segmentation, ultrasound images.

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Authors: Somayeh Sadeghi, Hamid A. Jalab, Sajjad Dadkhah

Abstract:

Due to availability of powerful image processing software and improvement of human computer knowledge, it becomes easy to tamper images. Manipulation of digital images in different fields like court of law and medical imaging create a serious problem nowadays. Copy-move forgery is one of the most common types of forgery which copies some part of the image and pastes it to another part of the same image to cover an important scene. In this paper, a copy-move forgery detection method proposed based on Fourier transform to detect forgeries. Firstly, image is divided to same size blocks and Fourier transform is performed on each block. Similarity in the Fourier transform between different blocks provides an indication of the copy-move operation. The experimental results prove that the proposed method works on reasonable time and works well for gray scale and colour images. Computational complexity reduced by using Fourier transform in this method.

Keywords: Copy-Move forgery, Digital Forensics, Image Forgery.

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Authors: Y. Long, L. Liu, K. V. Branin

Abstract:

One challenge faced by procurement decision-maker during the acquisition process is how to compare similar products from different suppliers and allocate orders among different products or services. This work focuses on allocating orders among multiple suppliers considering rebate. The objective function is to minimize the total acquisition cost including purchasing cost and rebate benefit. Rebate benefit is complex and difficult to estimate at the ordering step. Rebate rules vary for different suppliers and usually change over time. In this work, we developed a system to collect the rebate policies, standardized the rebate policies and developed two-stage optimization models for ordering allocation. Rebate policy with multi-tiers is considered in modeling. The discontinuous cost function of rebate benefit is formulated for different scenarios. A piecewise linear function is used to approximate the discontinuous cost function of rebate benefit. And a Mixed Integer Programing (MIP) model is built for order allocation problem with multi-tier rebate. A case study is presented and it shows that our optimization model can reduce the total acquisition cost by considering rebate rules.

Keywords: Discontinuous cost function, mixed integer programming, optimization, procurement, rebate.

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Authors: Monika Chuchro

Abstract:

This paper presents a preliminary attempt to apply classification of time series using meta-clusters in order to improve the quality of regression models. In this case, clustering was performed as a method to obtain subgroups of time series data with normal distribution from the inflow into wastewater treatment plant data, composed of several groups differing by mean value. Two simple algorithms, K-mean and EM, were chosen as a clustering method. The Rand index was used to measure the similarity. After simple meta-clustering, a regression model was performed for each subgroups. The final model was a sum of the subgroups models. The quality of the obtained model was compared with the regression model made using the same explanatory variables, but with no clustering of data. Results were compared using determination coefficient (R2), measure of prediction accuracy- mean absolute percentage error (MAPE) and comparison on a linear chart. Preliminary results allow us to foresee the potential of the presented technique.

Keywords: Clustering, Data analysis, Data mining, Predictive models.

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Authors: Sarabjeet Singh Sidhu, Ajay Batish, Sanjeev Kumar

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This paper reports the optimal process conditions for machining of three different types of MMC’s 65vol%SiC/A356.2; 10vol%SiC-5vol%quartz/Al and 30vol%SiC/A359 using PMEDM process. MRR, TWR, SR and surface integrity were evaluated after each trial and contributing process parameters were identified. The four responses were then collectively optimized using TOPSIS and optimal process conditions were identified for each type of MMC. The density of reinforced particles shields the matrix material from spark energy hence the high MRR and SR was observed with lowest reinforced particle. TWR was highest with Cu-Gr electrode due to disintegration of the weakly bonded particles in the composite electrode. Each workpiece was examined for surface integrity and ranked as per severity of surface defects observed and their rankings were used for arriving at the most optimal process settings for each workpiece. 

Keywords: Metal matrix composites (MMCs), Metal removal rate (MRR), Surface roughness (SR), Surface integrity (SI), Tool wear rate (TWR), Technique for order preference by similarity to ideal solution (TOPSIS).

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Authors: Subramaniam Chandran

Abstract:

This paper examines the interplay of policy options and cost-effective technology in providing sustainable distance education. A case study has been conducted among the learners and teachers. The emergence of learning technologies through CD, internet, and mobile is increasingly adopted by distance institutes for quick delivery and cost-effective factors. Their sustainability is conditioned by the structure of learners and well as the teaching community. The structure of learners in terms of rural and urban background revealed similarity in adoption and utilization of mobile learning. In other words, the technology transcended the rural-urban dichotomy. The teaching community was divided into two groups on policy issues. This study revealed both cost-effective as well as sustainability impacts on different learners groups divided by rural and urban location.

Keywords: Distance Education, Mobile Learning, Policy, Technology

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Authors: J. Bouchard, B. Prosperi, G. Bavre, M. Daudé, E. Jeandupeux

Abstract:

Young patients suffering from Cerebral Palsy are facing difficult choices concerning heavy surgeries. Diagnosis settled by surgeons can be complex and on the other hand decision for patient about getting or not such a surgery involves important reflection effort. Proposed software combining prediction for surgeries and post surgery kinematic values, and from 3D model representing the patient is an innovative tool helpful for both patients and medicine professionals. Beginning with analysis and classification of kinematics values from Data Base extracted from gait analysis in 3 separated clusters, it is possible to determine close similarity between patients. Prediction surgery best adapted to improve a patient gait is then determined by operating a suitable preconditioned neural network. Finally, patient 3D modeling based on kinematic values analysis, is animated thanks to post surgery kinematic vectors characterizing the closest patient selected from patients clustering.

Keywords: Cerebral Palsy, Clustering, Crouch Gait, 3-D Modeling.

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Authors: Wanhyun Cho, Soonja Kang, Sangkyoon Kim, Soonyoung Park

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In this paper, we present the human action recognition method using the variational Bayesian HMM with the Dirichlet process mixture (DPM) of the Gaussian-Wishart emission model (GWEM). First, we define the Bayesian HMM based on the Dirichlet process, which allows an infinite number of Gaussian-Wishart components to support continuous emission observations. Second, we have considered an efficient variational Bayesian inference method that can be applied to drive the posterior distribution of hidden variables and model parameters for the proposed model based on training data. And then we have derived the predictive distribution that may be used to classify new action. Third, the paper proposes a process of extracting appropriate spatial-temporal feature vectors that can be used to recognize a wide range of human behaviors from input video image. Finally, we have conducted experiments that can evaluate the performance of the proposed method. The experimental results show that the method presented is more efficient with human action recognition than existing methods.

Keywords: Human action recognition, Bayesian HMM, Dirichlet process mixture model, Gaussian-Wishart emission model, Variational Bayesian inference, Prior distribution and approximate posterior distribution, KTH dataset.

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Authors: Yohannes Yirga, Daniel Tesfay

Abstract:

The convective heat and mass transfer in nanofluid flow through a porous media due to a permeable stretching sheet with magnetic field, viscous dissipation, chemical reaction and Soret effects are numerically investigated. Two types of nanofluids, namely Cu-water and Ag-water were studied. The governing boundary layer equations are formulated and reduced to a set of ordinary differential equations using similarity transformations and then solved numerically using the Keller box method. Numerical results are obtained for the skin friction coefficient, Nusselt number and Sherwood number as well as for the velocity, temperature and concentration profiles for selected values of the governing parameters. Excellent validation of the present numerical results has been achieved with the earlier linearly stretching sheet problems in the literature.

Keywords: Heat and mass transfer, magnetohydrodynamics, nanofluid.

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Authors: N. Samarinas, C. Evangelides, C. Vrekos

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The aim of this paper is the comparison of three different methods, in order to produce fuzzy tolerance relations for rainfall data classification. More specifically, the three methods are correlation coefficient, cosine amplitude and max-min method. The data were obtained from seven rainfall stations in the region of central Greece and refers to 20-year time series of monthly rainfall height average. Three methods were used to express these data as a fuzzy relation. This specific fuzzy tolerance relation is reformed into an equivalence relation with max-min composition for all three methods. From the equivalence relation, the rainfall stations were categorized and classified according to the degree of confidence. The classification shows the similarities among the rainfall stations. Stations with high similarity can be utilized in water resource management scenarios interchangeably or to augment data from one to another. Due to the complexity of calculations, it is important to find out which of the methods is computationally simpler and needs fewer compositions in order to give reliable results.

Keywords: Classification, fuzzy logic, tolerance relations, rainfall data.

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Authors: Sang-Hun Lee, Bum-Soo Kim, Yang-Sae Moon, Jinho Kim

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In this paper we present a photo mosaic smartphone application in client-server based large-scale image databases. Photo mosaic is not a new concept, but there are very few smartphone applications especially for a huge number of images in the client-server environment. To support large-scale image databases, we first propose an overall framework working as a client-server model. We then present a concept of image-PAA features to efficiently handle a huge number of images and discuss its lower bounding property. We also present a best-match algorithm that exploits the lower bounding property of image-PAA. We finally implement an efficient Android-based application and demonstrate its feasibility.

Keywords: smartphone applications; photo mosaic; similarity search; data mining; large-scale image databases.

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Authors: Po-Jen Su, Huann-Ming Chou

Abstract:

In this article, we used the residual correction method to deal with transient thermoelastic problems with a hollow spherical region when the continuum medium possesses spherically isotropic thermoelastic properties. Based on linear thermoelastic theory, the equations of hyperbolic heat conduction and thermoelastic motion were combined to establish the thermoelastic dynamic model with consideration of the deformation acceleration effect and non-Fourier effect under the condition of transient thermal shock. The approximate solutions of temperature and displacement distributions are obtained using the residual correction method based on the maximum principle in combination with the finite difference method, making it easier and faster to obtain upper and lower approximations of exact solutions. The proposed method is found to be an effective numerical method with satisfactory accuracy. Moreover, the result shows that the effect of transient thermal shock induced by deformation acceleration is enhanced by non-Fourier heat conduction with increased peak stress. The influence on the stress increases with the thermal relaxation time.

Keywords: Maximum principle, non-Fourier heat conduction, residual correction method, thermo-elastic response.

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Authors: A. Gorin

Abstract:

The lecture represents significant advances in understanding of the transfer processes mechanism in turbulent separated flows. Based upon experimental data suggesting the governing role of generated local pressure gradient that takes place in the immediate vicinity of the wall in separated flow as a result of intense instantaneous accelerations induced by large-scale vortex flow structures similarity laws for mean velocity and temperature and spectral characteristics and heat and mass transfer law for turbulent separated flows have been developed. These laws are confirmed by available experimental data. The results obtained were employed for analysis of heat and mass transfer in some very complex processes occurring in technological applications such as impinging jets, heat transfer of cylinders in cross flow and in tube banks, packed beds where processes manifest distinct properties which allow them to be classified under turbulent separated flows. Many facts have got an explanation for the first time.

Keywords: impinging jets, packed beds, turbulent separatedflows, 'two-thirds power law'

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Authors: Fátima Gonçalves, Joaquim Brigas, Jorge Gonçalves

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As a way of managing the increasing volume and complexity of information that circulates in the present time, graphical representations are increasingly used, which add meaning to the information presented in communication media, through an efficient communication design. The visual culture itself, driven by technological evolution, has been redefining the forms of communication, so that contemporary visual communication represents a major impact on society. This article presents the results and respective comparative analysis of four publications in the Iberian press, focusing on the formal aspects of newspapers and the space they dedicate to the various communication elements. Two Portuguese newspapers and two Spanish newspapers were selected for this purpose. The findings indicated that the newspapers show a similarity in the use of graphic solutions, which corroborate a visual trend in communication design. The results also reveal that Spanish newspapers are more meticulous with graphic consistency. This study intended to contribute to improving knowledge of the Iberian generalist press.

Keywords: Communication design, graphic resources, Iberian Press, visual journalism.

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Authors: F. Meskine, N. Taleb, M. Chikr El-Mezouar, K. Kpalma, A. Almhdie

Abstract:

Image registration is the process of establishing point by point correspondence between images obtained from a same scene. This process is very useful in remote sensing, medicine, cartography, computer vision, etc. Then, the task of registration is to place the data into a common reference frame by estimating the transformations between the data sets. In this work, we develop a rigid point registration method based on the application of genetic algorithms and Hausdorff distance. First, we extract the feature points from both images based on the algorithm of global and local curvature corner. After refining the feature points, we use Hausdorff distance as similarity measure between the two data sets and for optimizing the search space we use genetic algorithms to achieve high computation speed for its inertial parallel. The results show the efficiency of this method for registration of satellite images.

Keywords: Feature extraction, Genetic algorithms, Hausdorff distance, Image registration, Point registration.

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Authors: Siti Khuzaimah Soid, Zanariah Mohd Yusof, Ahmad Sukri Abd Aziz, Seripah Awang Kechil

Abstract:

A steady two-dimensional magnetohydrodynamics flow and heat transfer over a stretching vertical sheet influenced by radiation and porosity is studied. The governing boundary layer equations of partial differential equations are reduced to a system of ordinary differential equations using similarity transformation. The system is solved numerically by using a finite difference scheme known as the Keller-box method for some values of parameters, namely the radiation parameter N, magnetic parameter M, buoyancy parameter l , Prandtl number Pr and permeability parameter K. The effects of the parameters on the heat transfer characteristics are analyzed and discussed. It is found that both the skin friction coefficient and the local Nusselt number decrease as the magnetic parameter M and permeability parameter K increase. Heat transfer rate at the surface decreases as the radiation parameter increases.

Keywords: Keller-box, MHD boundary layer flow, permeability stretching.

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Authors: Indra Budi, Rizal Fathoni Aji, Agus Widodo

Abstract:

Prediction of future research topics by using time series analysis either statistical or machine learning has been conducted previously by several researchers. Several methods have been proposed to combine the forecasting results into single forecast. These methods use fixed combination of individual forecast to get the final forecast result. In this paper, quite different approach is employed to select the forecasting methods, in which every point to forecast is calculated by using the best methods used by similar validation dataset. The dataset used in the experiment is time series derived from research report in Garuda, which is an online sites belongs to the Ministry of Education in Indonesia, over the past 20 years. The experimental result demonstrates that the proposed method may perform better compared to the fix combination of predictors. In addition, based on the prediction result, we can forecast emerging research topics for the next few years.

Keywords: Combination, emerging topics, ensemble, forecasting, machine learning, prediction, research topics, similarity measure, time series.

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Authors: Nichole Haag, Kimberly Velk, Tyler McCune, Chun Wu

Abstract:

Methicillin/multiple-resistant Staphylococcus aureus (MRSA) are infectious bacteria that are resistant to common antibiotics. A previous in silico study in our group has identified a hypothetical protein SAV1226 as one of the potential drug targets. In this study, we reported the bioinformatics characterization, as well as cloning, expression, purification and kinetic assays of hypothetical protein SAV1226 from methicillin/vancomycin-resistant Staphylococcus aureus Mu50 strain. MALDI-TOF/MS analysis revealed a low degree of structural similarity with known proteins. Kinetic assays demonstrated that hypothetical protein SAV1226 is neither a domain of an ATP dependent dihydroxyacetone kinase nor of a phosphotransferase system (PTS) dihydroxyacetone kinase, suggesting that the function of hypothetical protein SAV1226 might be misannotated on public databases such as UniProt and InterProScan 5.

Keywords: Dihydroxyacetone kinase, essential genes, Methicillin-resistant Staphylococcus aureus, drug target.

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Authors: Phakamas Rachamontree, Theerawut Phusantisampan, Natthakorn Woravutthikul, Peerapong Pornwongthong, Malinee Sriariyanun

Abstract:

A total of 115 yeast strains isolated from local cassava processing wastes were measured for crude protein content. Among these strains, the strain MSY-2 possessed the highest protein concentration (>3.5 mg protein/mL). By using molecular identification tools, it was identified to be a strain of Pichia kudriavzevii based on similarity of D1/D2 domain of 26S rDNA region. In this study, to optimize the protein production by MSY-2 strain, Response Surface Methodology (RSM) was applied. The tested parameters were the carbon content, nitrogen content, and incubation time. Here, the value of regression coefficient (R2) = 0.7194 could be explained by the model which is high to support the significance of the model. Under the optimal condition, the protein content was produced up to 3.77 g per L of the culture and MSY-2 strain contains 66.8 g protein per 100 g of cell dry weight. These results revealed the plausibility of applying the novel strain of yeast in single-cell protein production.

Keywords: Single cell protein, response surface methodology, yeast, cassava processing waste.

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