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Selection of Pichia kudriavzevii Strain for the Production of Single-Cell Protein from Cassava Processing Waste

Authors: Phakamas Rachamontree, Theerawut Phusantisampan, Natthakorn Woravutthikul, Peerapong Pornwongthong, Malinee Sriariyanun


A total of 115 yeast strains isolated from local cassava processing wastes were measured for crude protein content. Among these strains, the strain MSY-2 possessed the highest protein concentration (>3.5 mg protein/mL). By using molecular identification tools, it was identified to be a strain of Pichia kudriavzevii based on similarity of D1/D2 domain of 26S rDNA region. In this study, to optimize the protein production by MSY-2 strain, Response Surface Methodology (RSM) was applied. The tested parameters were the carbon content, nitrogen content, and incubation time. Here, the value of regression coefficient (R2) = 0.7194 could be explained by the model which is high to support the significance of the model. Under the optimal condition, the protein content was produced up to 3.77 g per L of the culture and MSY-2 strain contains 66.8 g protein per 100 g of cell dry weight. These results revealed the plausibility of applying the novel strain of yeast in single-cell protein production.

Keywords: Single cell protein, response surface methodology, yeast, cassava processing waste.

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[1] O. Chaisinboon, and J. Chontanawat, "Factors determining the competing use of Thailand's cassava for food and fuel," Energy Procedia, vol. 9, 2011, pp. 216-229.
[2] T. Silalertruksa, S.H. Gheewala, K. Hunecke, and U.R. Fritsche, "Biofuels and employment effects: Implications for socio-economic development in Thailand," Biomass and Bioenergy, vol. 46, 2012, pp. 409-418.
[3] M. Davis, C.V. Maxwell, and T.J. Wistuba, "Dietary supplementation with phosphorylate mannans improve growth responses and modulates immune function of weanling pig," Journal of Animal Science, vol. 84, 2004, pp. 1882-1891.
[4] Anupama, and P. Ravindra, "Value-added food single cell protein," Biotechnol Adv, vol. 18, 2000, pp. 459-479.
[5] A. Chatzifragkou, A. Makri, A. Belka, and S. Bellou, "Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species," Energy, vol. 36, 2011, pp. 1097-1108.
[6] C. Evans, and C. Ratledge, "A comparison of the oleaginous yeast Candida curvata, grown on different carbon sources in continuous and batch culture," Lipids, vol.18, 1983, pp. 623-629.
[7] J. Yadav, J. Bezawada, S. Elharche, S. Yan, R. Tyagi, and R. Surampalli, "Simultaneous single-cell protein production and COD removal with characterization of residual protein," Bioprocess Biosyst Eng, vol. 37(6), 2013, pp. 1017-1029.
[8] J. Yadav, J. Bezawada, C. Ajila, S. Yan, R. Tyagi, and R. Surampalli, "Mixed culture of Kluyveromyces marxianus and Candida krusei for single-cell protein production and organic load removal from whey," Biores Technol, vol.164, 2014, pp. 119-127.
[9] M. Bradford, "Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding," Anal Biochem, vol.72, 1976, pp. 248–254.
[10] C. Kurtzman, and C.J. Robnett, "Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences," Antonie van Leeuwenhoek, vol. 73(4), 1998, pp. 331-371.
[11] S. Bringans, S. Eriksen, T. Kendrick, P. Gopalakrishnakone, A. Livk, and R. Lock, "Proteomic analyses of the venom of Heterometrus longimanus (Asian black scorpion)," Proteomics, vol. 8, 2008, pp. 1081- 1096.
[12] G. Chan, H.M. Gan, H.L. Ling, and N.A. Rashid, "Genome sequence of Pichia kudriavzevii M12, a potential producer of bioethanol and phytase," Eukaryot Cell, vol. 11(10), 2012, pp. 1300-1301.
[13] S. Moon, M. Chang, H.Y. Kim, and H.C. Chang, "Pichia kudriavzevii is the major yeast involved in film-formation, off-odor production, and texture-softening in over-ripened Kimchi," Food Science and Biotechnology, vol. 23(2), 2014, pp. 489-497.
[14] N. Yuangsaard, W. Yongmanitchai, M. Yamada, and S. Limtong, "Selection and characterization of a newly isolated thermotolerant Pichia kudriavzevii strain for ethanol production at high temperature from cassava starch hydrolysate," Antonie Van Leeuwenhoek, vol. 103(3), 2013, pp. 577-588.