%0 Journal Article
	%A Phakamas Rachamontree and  Theerawut Phusantisampan and  Natthakorn Woravutthikul and  Peerapong Pornwongthong and  Malinee Sriariyanun
	%D 2015
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 101, 2015
	%T Selection of Pichia kudriavzevii Strain for the Production of Single-Cell Protein from Cassava Processing Waste
	%U https://publications.waset.org/pdf/10001349
	%V 101
	%X A total of 115 yeast strains isolated from local cassava
processing wastes were measured for crude protein content. Among
these strains, the strain MSY-2 possessed the highest protein
concentration (>3.5 mg protein/mL). By using molecular
identification tools, it was identified to be a strain of Pichia
kudriavzevii based on similarity of D1/D2 domain of 26S rDNA
region. In this study, to optimize the protein production by MSY-2
strain, Response Surface Methodology (RSM) was applied. The
tested parameters were the carbon content, nitrogen content, and
incubation time. Here, the value of regression coefficient (R2) =
0.7194 could be explained by the model which is high to support the
significance of the model. Under the optimal condition, the protein
content was produced up to 3.77 g per L of the culture and MSY-2
strain contains 66.8 g protein per 100 g of cell dry weight. These
results revealed the plausibility of applying the novel strain of yeast
in single-cell protein production.
	%P 517 - 521