Search results for: soil microbial activity
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 9268

Search results for: soil microbial activity

9028 Development IoT System for Smart Maize Production in Nigeria

Authors: Oyenike M. Olanrewaju, Faith O. Echobu, Aderemi G. Adesoji, Emmy Danny Ajik, Joseph Nda Ndabula, Stephen Luka

Abstract:

Nutrients are required for any soil with which plants thrive to improve efficient growth and productivity. Amongst these nutrients required for proper plant productivity are nitrogen, phosphorus and potassium (NPK). Due to factors like leaching, nutrient uptake by plants, soil erosion and evaporation, these elements tend to be in low quantity and the need to replenish them arises. However, this replenishment of soil nutrients cannot be done without a timely soil test to enable farmers to know the amount of each element in short quantity and evaluate the amount required to be added. Though wet soil analysis is good, it comes with a lot of challenges ranging from soil test gargets availability to the technical knowledge of how to conduct such soil tests by the common farmer. In this research, an Internet of Things test kit was developed to fill in the gaps created by wet soil analysis. The kit comprises components that were used to measure Nitrogen, Phosphorous and potassium (N, P, K) soil content, soil temperature and soil moisture at a series of intervals. In this implementation, the fieldwork was carried out within 0.2 hectares of land divided into smaller plots. Nitrogen values from the three reps range from 14.8 – 15mg/kg, Phosphorous 20.2-21.4 mg/kg, and Potassium 50.2-53 mg/kg. This information with soil moisture information obtained enabled the farmers to make informed and precise decisions on fertilizer applications, and wastage was avoided.

Keywords: internet of things, soil Nutrients, test kit, soil temperature

Procedia PDF Downloads 24
9027 Numerical Investigations on Group Piles’ Lateral Bearing Capacity Considering Interaction of Soil and Structure

Authors: Mahdi Sadeghian, Mahmoud Hassanlourad, Alireza Ardakani, Reza Dinarvand

Abstract:

In this research, the behavior of monopiles, under lateral loads, was investigated with vertical and oblique piles by Finite Element Method. In engineering practice when soil-pile interaction comes to the picture some simplifications are applied to reduce the design time. As a simplified replacement of soil and pile interaction analysis, pile could be replaced by a column. The height of the column would be equal to the free length of the pile plus a portion of the embedded length of it. One of the important factors studied in this study was that columns with an equivalent length (free length plus a part of buried depth) could be used instead of soil and pile modeling. The results of the analysis show that the more internal friction angle of the soil increases, the more the bearing capacity of the soil is achieved. This additional length is 6 to 11 times of the pile diameter in dense soil although in loose sandy soil this range might increase.

Keywords: Depth of fixity, Lateral bearing capacity, Oblique pile, Pile group, Soil-structure interaction

Procedia PDF Downloads 194
9026 Lipid Extraction from Microbial Cell by Electroporation Technique and Its Influence on Direct Transesterification for Biodiesel Synthesis

Authors: Abu Yousuf, Maksudur Rahman Khan, Ahasanul Karim, Amirul Islam, Minhaj Uddin Monir, Sharmin Sultana, Domenico Pirozzi

Abstract:

Traditional biodiesel feedstock like edible oils or plant oils, animal fats and cooking waste oil have been replaced by microbial oil in recent research of biodiesel synthesis. The well-known community of microbial oil producers includes microalgae, oleaginous yeast and seaweeds. Conventional transesterification of microbial oil to produce biodiesel is lethargic, energy consuming, cost-ineffective and environmentally unhealthy. This process follows several steps such as microbial biomass drying, cell disruption, oil extraction, solvent recovery, oil separation and transesterification. Therefore, direct transesterification of biodiesel synthesis has been studying for last few years. It combines all the steps in a single reactor and it eliminates the steps of biomass drying, oil extraction and separation from solvent. Apparently, it seems to be cost-effective and faster process but number of difficulties need to be solved to make it large scale applicable. The main challenges are microbial cell disruption in bulk volume and make faster the esterification reaction, because water contents of the medium sluggish the reaction rate. Several methods have been proposed but none of them is up to the level to implement in large scale. It is still a great challenge to extract maximum lipid from microbial cells (yeast, fungi, algae) investing minimum energy. Electroporation technique results a significant increase in cell conductivity and permeability caused due to the application of an external electric field. Electroporation is required to alter the size and structure of the cells to increase their porosity as well as to disrupt the microbial cell walls within few seconds to leak out the intracellular lipid to the solution. Therefore, incorporation of electroporation techniques contributed in direct transesterification of microbial lipids by increasing the efficiency of biodiesel production rate.

Keywords: biodiesel, electroporation, microbial lipids, transesterification

Procedia PDF Downloads 243
9025 Physicochemistry of Pozzolanic Stabilization of a Class A-2-7 Lateritic Soil

Authors: Ahmed O. Apampa, Yinusa A. Jimoh

Abstract:

The paper examines the mechanism of pozzolan-soil reactions, using a recent study on the chemical stabilization of a Class A-2-7 (3) lateritic soil, with corn cob ash (CCA) as case study. The objectives are to establish a nexus between cation exchange capacity of the soil, the alkaline forming compounds in CCA and percentage CCA addition to soil beyond which no more improvement in strength properties can be achieved; and to propose feasible chemical reactions to explain the chemical stabilization of the lateritic soil with CCA alone. The lateritic soil, as well as CCA of pozzolanic quality Class C were separately analysed for their metallic oxide composition using the X-Ray Fluorescence technique. The cation exchange capacity (CEC) of the soil and the CCA were computed theoretically using the percentage composition of the base cations Ca2+, Mg2+ K+ and Na2+ as 1.48 meq/100 g and 61.67 meq/100 g respectively, thus indicating a ratio of 0.024 or 2.4%. This figure, taken as the theoretical amount required to just fill up the exchangeable sites of the clay molecules, compares well with the laboratory observation of 1.5% for the optimum level of CCA addition to lateritic soil. The paper went on to present chemical reaction equations between the alkaline earth metals in the CCA and the silica in the lateritic soil to form silicates, thereby proposing an extension of the theory of mechanism of soil stabilization to cover chemical stabilization with pozzolanic ash only. The paper concluded by recommending further research on the molecular structure of soils stabilized with pozzolanic waste ash alone, with a view to confirming the chemical equations advanced in the study.

Keywords: cation exchange capacity, corn cob ash, lateritic soil, soil stabilization

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9024 Influence of Nano Copper Slag in Strength Behavior of Lime Stabilized Soil

Authors: V. K. Stalin, M. Kirithika, K. Shanmugam, K. Tharini

Abstract:

Nanotechnology has been widely used in many applications such as medical, electronics, robotics and also in geotechnical engineering area through stabilization of bore holes, grouting etc. In this paper, an attempt is made for understanding the influence of nano copper slag (1%, 2% & 3%) on the index, compaction and UCC strength properties of natural soil (CH type) with and without lime stabilization for immediate and 7 days curing period. Results indicated that upto 1% of Nano copper slag, there is an increment in UC strength of virgin soil and lime stabilised soil. Beyond 1% nano copper slag, there is a steep reduction in UC strength and increase of plasticity both in lime stabilised soil and virgin soil. The effect of lime is found to show more influence on large surface area of nano copper slag in natural soil. For both immediate and curing effect, with 1% of Nano copper slag, the maximum unconfined compressive strength was 38% and 106% higher than that of the virgin soil strength.

Keywords: lime, nano copper slag, SEM, XRD, stabilisation

Procedia PDF Downloads 381
9023 Developing a Town Based Soil Database to Assess the Sensitive Zones in Nutrient Management

Authors: Sefa Aksu, Ünal Kızıl

Abstract:

For this study, a town based soil database created in Gümüşçay District of Biga Town, Çanakkale, Turkey. Crop and livestock production are major activities in the district. Nutrient management is mainly based on commercial fertilizer application ignoring the livestock manure. Within the boundaries of district, 122 soil sampling points determined over the satellite image. Soil samples collected from the determined points with the help of handheld Global Positioning System. Labeled samples were sent to a commercial laboratory to determine 11 soil parameters including salinity, pH, lime, organic matter, nitrogen, phosphorus, potassium, iron, manganese, copper and zinc. Based on the test results soil maps for mentioned parameters were developed using remote sensing, GIS, and geostatistical analysis. In this study we developed a GIS database that will be used for soil nutrient management. Methods were explained and soil maps and their interpretations were summarized in the study.

Keywords: geostatistics, GIS, nutrient management, soil mapping

Procedia PDF Downloads 346
9022 SPR Immunosensor for the Detection of Staphylococcus aureus

Authors: Muhammad Ali Syed, Arshad Saleem Bhatti, Chen-zhong Li, Habib Ali Bokhari

Abstract:

Surface plasmon resonance (SPR) biosensors have emerged as a promising technique for bioanalysis as well as microbial detection and identification. Real time, sensitive, cost effective, and label free detection of biomolecules from complex samples is required for early and accurate diagnosis of infectious diseases. Like many other types of optical techniques, SPR biosensors may also be successfully utilized for microbial detection for accurate, point of care, and rapid results. In the present study, we have utilized a commercially available automated SPR biosensor of BI company to study the microbial detection form water samples spiked with different concentration of Staphylococcus aureus bacterial cells. The gold thin film sensor surface was functionalized to react with proteins such as protein G, which was used for directed immobilization of monoclonal antibodies against Staphylococcus aureus. The results of our work reveal that this immunosensor can be used to detect very small number of bacterial cells with higher sensitivity and specificity. In our case 10^3 cells/ml of water have been successfully detected. Therefore, it may be concluded that this technique has a strong potential to be used in microbial detection and identification.

Keywords: surface plasmon resonance (SPR), Staphylococcus aureus, biosensors, microbial detection

Procedia PDF Downloads 444
9021 Delineation of Soil Physical Properties Using Electrical Conductivity, Case Study: Volcanic Soil Simulation Model

Authors: Twin Aji Kusumagiani, Eleonora Agustine, Dini Fitriani

Abstract:

The value changes of soil physical properties in the agricultural area are giving impacts on soil fertility. This can be caused by excessive usage of inorganic fertilizers and imbalances on organic fertilization. Soil physical parameters that can be measured include soil electrical conductivity, water content volume, soil porosity, dielectric permittivity, etc. This study used the electrical conductivity and volume water content as the measured physical parameters. The study was conducted on volcanic soil obtained from agricultural land conditioned with NPK fertilizer and salt in a certain amount. The dimension of the conditioned soil being used is 1 x 1 x 0.5 meters. By using this method, we can delineate the soil electrical conductivity value of land due to changes in the provision of inorganic NPK fertilizer and the salinity in the soil. Zone with the additional 1 kg of salt has the dimension of 60 cm in width, 20 cm in depth and 1 cm in thickness while zone with the additional of 10 kg NPK fertilizer has the dimensions of 70 cm in width, 20 cm in depth and 3 cm in thickness. This salt addition resulted in EC values changes from the original condition. Changes of the EC value tend to occur at a depth of 20 to 40 cm on the line 1B at 9:45 dS/cm and line 1C of 9.35 dS/cm and tend to have the direction to the Northeast.

Keywords: EC, electrical conductivity, VWC, volume water content, NPK fertilizer, salt, volcanic soil

Procedia PDF Downloads 285
9020 Utilization of Logging Residue to Reduce Soil Disturbance of Timber Harvesting

Authors: Juang R. Matangaran, Qi Adlan

Abstract:

Industrial plantation forest in Indonesia was developed in 1983, and since then, several companies have been successfully planted a total area of concessionaire approximately 10 million hectares. Currently, these plantation forests have their annual harvesting period. In the timber harvesting process, amount part of the trees generally become logging residue. Tree parts such as branches, twigs, defected stem and leaves are unused section of tree on the ground after timber harvesting. The use of heavy machines in timber harvesting area has caused damage to the forest soil. The negative impact of such machines includes loss of topsoil, soil erosion, and soil compaction. Forest soil compaction caused reduction of forest water infiltration, increase runoff and causes difficulty for root penetration. In this study, we used logging residue as soil covers on the passages passed by skidding machines in order to observe the reduction soil compaction. Bulk density of soil was measured and analyzed after several times of skidding machines passage on skid trail. The objective of the research was to analyze the effect of logging residue on reducing soil compaction. The research was taken place at one of the industrial plantation forest area of South Sumatra Indonesia. The result of the study showed that percentage increase of soil compaction bare soil was larger than soil surface covered by logging residue. The maximum soil compaction occurred after 4 to 5 passes on soil without logging residue or bare soil and after 7 to 8 passes on soil cover by logging residue. The use of logging residue coverings could reduce soil compaction from 45% to 60%. The logging residue was effective in decreasing soil disturbance of timber harvesting at the plantation forest area.

Keywords: bulk density, logging residue, plantation forest, soil compaction, timber harvesting

Procedia PDF Downloads 373
9019 Measurement of Greenhouse Gas Emissions from Sugarcane Plantation Soil in Thailand

Authors: Wilaiwan Sornpoon, Sébastien Bonnet, Savitri Garivait

Abstract:

Continuous measurements of greenhouse gases (GHGs) emitted from soils are required to understand diurnal and seasonal variations in soil emissions and related mechanism. This understanding plays an important role in appropriate quantification and assessment of the overall change in soil carbon flow and budget. This study proposes to monitor GHGs emissions from soil under sugarcane cultivation in Thailand. The measurements were conducted over 379 days. The results showed that the total net amount of GHGs emitted from sugarcane plantation soil amounts to 36 Mg CO2eq ha-1. Carbon dioxide (CO2) and nitrous oxide (N2O) were found to be the main contributors to the emissions. For methane (CH4), the net emission was found to be almost zero. The measurement results also confirmed that soil moisture content and GHGs emissions are positively correlated.

Keywords: soil, GHG emission, sugarcane, agriculture, Thailand

Procedia PDF Downloads 398
9018 Spatial Variability of Soil Metal Contamination to Detect Cancer Risk Zones in Coimbatore Region of India

Authors: Aarthi Mariappan, Janani Selvaraj, P. B. Harathi, M. Prashanthi Devi

Abstract:

Anthropogenic modification of the urban environment has largely increased in the recent years in order to sustain the growing human population. Intense industrial activity, permanent and high traffic on the roads, a developed subterranean infrastructure network, land use patterns are just some specific characteristics. Every day, the urban environment is polluted by more or less toxic emissions, organic or metals wastes discharged from specific activities such as industrial, commercial, municipal. When these eventually deposit into the soil, the physical and chemical properties of the surrounding soil is changed, transforming it into a human exposure indicator. Metals are non-degradable and occur cumulative in soil due to regular deposits are a result of permanent human activity. Due to this, metals are a contaminant factor for soil when persistent over a long period of time and a possible danger for inhabitant’s health on prolonged exposure. Metals accumulated in contaminated soil may be transferred to humans directly, by inhaling the dust raised from top soil, or by ingesting, or by dermal contact and indirectly, through plants and animals grown on contaminated soil and used for food. Some metals, like Cu, Mn, Zn, are beneficial for human’s health and represent a danger only if their concentration is above permissible levels, but other metals, like Pb, As, Cd, Hg, are toxic even at trace level causing gastrointestinal and lung cancers. In urban areas, metals can be emitted from a wide variety of sources like industrial, residential, commercial activities. Our study interrogates the spatial distribution of heavy metals in soil in relation to their permissible levels and their association with the health risk to the urban population in Coimbatore, India. Coimbatore region is a high cancer risk zone and case records of gastro intestinal and respiratory cancer patients were collected from hospitals and geocoded in ArcGIS10.1. The data of patients pertaining to the urban limits were retained and checked for their diseases history based on their diagnosis and treatment. A disease map of cancer was prepared to show the disease distribution. It has been observed that in our study area Cr, Pb, As, Fe and Mg exceeded their permissible levels in the soil. Using spatial overlay analysis a relationship between environmental exposure to these potentially toxic elements in soil and cancer distribution in Coimbatore district was established to show areas of cancer risk. Through this, our study throws light on the impact of prolonged exposure to soil contamination in soil in the urban zones, thereby exploring the possibility to detect cancer risk zones and to create awareness among the exposed groups on cancer risk.

Keywords: soil contamination, cancer risk, spatial analysis, India

Procedia PDF Downloads 380
9017 Plant Microbiota of Coastal Halophyte Salicornia Ramossisima

Authors: Isabel N. Sierra-Garcia, Maria J. Ferreira, Sandro Figuereido, Newton Gomes, Helena Silva, Angela Cunha

Abstract:

Plant-associated microbial communities are considered crucial in the adaptation of halophytes to coastal environments. The plant microbiota can be horizontally acquired from the environment or vertically transmitted from generation to generation via seeds. Recruiting of the microbial communities by the plant is affected by geographical location, soil source, host genotype, and cultivation practice. There is limited knowledge reported on the microbial communities in halophytes the influence of biotic and abiotic factors. In this work, the microbiota associated with the halophyte Salicornia ramosissima was investigated to determine whether the structure of bacterial communities is influenced by host genotype or soil source. For this purpose, two contrasting sites where S. ramosissima is established in the estuarine system of the Ria de Aveiro were investigated. One site corresponds to a natural salt marsh where S. ramosissima plants are present (wild plants), and the other site is a former salt pan that nowadays are subjected to intensive crop production of S. ramosissima (crop plants). Bacterial communities from the rhizosphere, seeds and root endosphere of S. ramossisima from both sites were investigated by sequencing bacterial 16S rRNA gene using the Illumina MiSeq platform. The analysis of the sequences showed that the three plant-associated compartments, rhizosphere, root endosphere, and seed endosphere, harbor distinct microbiomes. However, bacterial richness and diversity were higher in seeds of wild plants, followed by rhizosphere in both sites, while seeds in the crop site had the lowest diversity. Beta diversity measures indicated that bacterial communities in root endosphere and seeds were more similar in both wild and crop plants in contrast to rhizospheres that differed by local, indicating that the recruitment of the similar bacterial communities by the plant genotype is active in regard to the site. Moreover, bacterial communities from the root endosphere and rhizosphere were phylogenetically more similar in both sites, but the phylogenetic composition of seeds in wild and crop sites was distinct. These results indicate that cultivation practices affect the seed microbiome. However, minimal vertical transmission of bacteria from seeds to adult plants is expected. Seeds from the crop site showed higher abundances of Kushneria and Zunongwangia genera. Bacterial members of the classes Alphaprotebacteria and Bacteroidia were the most ubiquitous across sites and compartments and might encompass members of the core microbiome. These findings indicate that bacterial communities associated with S. ramosissima are more influenced by host genotype rather than local abiotic factors or cultivation practices. This study provides a better understanding of the composition of the plant microbiota in S. ramosissima , which is essential to predict the interactions between plant and associated microbial communities and their effects on plant health. This knowledge is useful to the manipulations of these microbial communities to enhance the health and productivity of this commercially important plant.

Keywords: halophytes, plant microbiome, Salicornia ramosissima, agriculture

Procedia PDF Downloads 129
9016 Characteristics of Clayey Subgrade Soil Mixed with Cement Stabilizer

Authors: Manju, Praveen Aggarwal

Abstract:

Clayey soil is considered weakest subgrade soil from civil engineering point of view under moist condition. These swelling soils attract and absorb water and losses their strength. Certain inherent properties of these clayey soils need modification for their bulk use in the construction of highways/runways pavements and embankments, etc. In this paper, results of clayey subgrade modified with cement stabilizer is presented. Investigation includes evaluation of specific gravity, Atterberg’s limits, grain size distribution, maximum dry density, optimum moisture content and CBR value of the clayey soil and cement treated clayey soil. A series of proctor compaction and CBR tests (un-soaked and soaked) are carried out on clayey soil and clayey soil mixed with cement stabilizer in 2%, 4% & 6% percentages to the dry weight of soil. In CBR test, under soaked condition best results are obtained with 6% of cement. However, the difference between the CBR value by addition of 4% and 6% cement is not much. Therefore from economical consideration addition of 4% cement gives the best result after soaking period of 90 days.

Keywords: clayey soil, cement, maximum dry density, optimum moisture content, California bearing ratio

Procedia PDF Downloads 310
9015 An Improved Visible Range Absorption Spectroscopy on Soil Macronutrient

Authors: Suhaila Isaak, Yusmeeraz Yusof, Khairunnisa Mohd Yusof, Ahmad Safuan Abdul Rashid

Abstract:

Soil fertility is commonly evaluated by soil macronutrients such as nitrate, potassium, and phosphorus contents. Optical spectroscopy is an emerging technology which is rapid and simple has been widely used in agriculture to measure soil fertility. For visible and near infrared absorption spectroscopy, the absorbed light level in is useful for soil macro-nutrient measurement. This is because the absorption of light in a soil sample influences sensitivity of the measurement. This paper reports the performance of visible and near infrared absorption spectroscopy in the 400–1400 nm wavelength range using light-emitting diode as the excitation light source to predict the soil macronutrient content of nitrate, potassium, and phosphorus. The experimental results show an improved linear regression analysis of various soil specimens based on the Beer–Lambert law to determine sensitivity of soil spectroscopy by evaluating the absorption of characteristic peaks emitted from a light-emitting diode and detected by high sensitivity optical spectrometer. This would denote in developing a simple and low-cost soil spectroscopy with light-emitting diode for future implementation.

Keywords: macronutrients absorption, optical spectroscopy, soil, absorption

Procedia PDF Downloads 255
9014 Soil Degradation Processes in Marginal Uplands of Samar Island, Philippines

Authors: Dernie Taganna Olguera

Abstract:

Marginal uplands are fragile ecosystems in the tropics that need to be evaluated for sustainable utilization and land degradation mitigation. Thus, this study evaluated the dominant soil degradation processes in selected marginal uplands of Samar Island, Philippines; evaluated the important factors influencing soil degradation in the selected sites and identified the indicators of soil degradation in marginal uplands of the tropical landscape of Samar Island, Philippines. Two (2) sites were selected (Sta. Rita, Samar and Salcedo, Eastern, Samar) representing the western and eastern sides of Samar Island respectively. These marginal uplands represent different agro-climatic zones suitable for the study. Soil erosion is the major soil degradation process in the marginal uplands studied. It resulted in not only considerable soil losses but nutrient losses as well. Soil erosion varied with vegetation cover and site. It was much higher in the sweetpotato, cassava, and gabi crops than under natural vegetation. In addition, soil erosion was higher in Salcedo than in Sta. Rita, which is related to climatic and soil characteristics. Bulk density, porosity, aggregate stability, soil pH, organic matter, and carbon dioxide evolution are good indicators of soil degradation. The dominance of Saccharum spontaneum Linn., Imperata cylindrica Linn, Melastoma malabathricum Linn. and Psidium guajava Linn indicated degraded soil condition. Farmer’s practices particularly clean culture and organic fertilizer application influenced the degree of soil degradation in the marginal uplands of Samar Island, Philippines.

Keywords: soil degradation, soil erosion, marginal uplands, Samar island, Philippines

Procedia PDF Downloads 374
9013 The Effect of Conservative Tillage on Physical Properties of Soil and Yield of Rainfed Wheat

Authors: Abolfazl Hedayatipoor, Mohammad Younesi Alamooti

Abstract:

In order to study the effect of conservative tillage on a number of physical properties of soil and the yield of rainfed wheat, an experiment in the form of a randomized complete block design (RCBD) with three replications was conducted in a field in Aliabad County, Iran. The study treatments included: T1) Conventional method, T2) Combined moldboard plow method, T3) Chisel-packer method, and T4) Direct planting method. During early October, the study soil was prepared based on these treatments in a field which was used for rainfed wheat farming in the previous year. The apparent specific gravity of soil, weighted mean diameter (WMD) of soil aggregates, soil mechanical resistance, and soil permeability were measured. Data were analyzed in MSTAT-C. Results showed that the tillage practice had no significant effect on grain yield (p < 0.05). Soil permeability was 10.9, 16.3, 15.7 and 17.9 mm/h for T1, T2, T3 and T4, respectively.

Keywords: rainfed agriculture, conservative tillage, energy consumption, wheat

Procedia PDF Downloads 180
9012 The Influense of Alternative Farming Systems on Physical Parameters of the Soil

Authors: L. Masilionyte, S. Maiksteniene

Abstract:

Alternative farming systems are used to cultivate high quality food products and retain the viability and fertility of soil. The field experiments of different farming systems were conducted at Joniškėlis Experimental Station of the Lithuanian Research Centre for Agriculture and Forestry in 2006–2013. The soil of the experimental site was Endocalcari-Endohypogleyic Cambisol (CMg-n-w-can). In different farming systems, farmyard manure, straw and green manure catch crops used for fertilization both in the soil low in humus and in the soil moderate in humus. In the 0–20 cm depth layer, it had a more significant effect on soil moisture than on other physical soil properties. In the agricultural systems, in which catch crops had been grown, soil physical characteristics did not differ significantly before their biomass incorporation, except for the moisture content, which was lower in rainy periods and higher in drier periods than in the soil without catch crops. Soil bulk density and porosity in the topsoil layer were more dependent on soil humus content than on agricultural measures used: in the soil moderate in humus content, compared with the soil low in humus, bulk density was by 1.4 % lower, and porosity by 1.8 % higher. The research findings create a possibility to make improvements in alternative cropping systems by choosing organic fertilizers and catch crops’ combinations that have the sustainable effect on soil and that maintain the sustainability of soil productivity parameters. Rational fertilization systems, securing the stability of soil productivity parameters and crop rotation productivity will promote a development of organic agriculture.

Keywords: agro-measures, soil physical parameters, organic farming, sustainable farming

Procedia PDF Downloads 377
9011 Anaerobic Digestion Batch Study of Taxonomic Variations in Microbial Communities during Adaptation of Consortium to Different Lignocellulosic Substrates Using Targeted Sequencing

Authors: Priyanka Dargode, Suhas Gore, Manju Sharma, Arvind Lali

Abstract:

Anaerobic digestion has been widely used for production of methane from different biowastes. However, the complexity of microbial communities involved in the process is poorly understood. The performance of biogas production process concerning the process productivity is closely coupled to its microbial community structure and syntrophic interactions amongst the community members. The present study aims at understanding taxonomic variations occurring in any starter inoculum when acclimatised to different lignocellulosic biomass (LBM) feedstocks relating to time of digestion. The work underlines use of high throughput Next Generation Sequencing (NGS) for validating the changes in taxonomic patterns of microbial communities. Biomethane Potential (BMP) batches were set up with different pretreated and non-pretreated LBM residues using the same microbial consortium and samples were withdrawn for studying the changes in microbial community in terms of its structure and predominance with respect to changes in metabolic profile of the process. DNA of samples withdrawn at different time intervals with reference to performance changes of the digestion process, was extracted followed by its 16S rRNA amplicon sequencing analysis using Illumina Platform. Biomethane potential and substrate consumption was monitored using Gas Chromatography(GC) and reduction in COD (Chemical Oxygen Demand) respectively. Taxonomic analysis by QIIME server data revealed that microbial community structure changes with different substrates as well as at different time intervals. It was observed that biomethane potential of each substrate was relatively similar but, the time required for substrate utilization and its conversion to biomethane was different for different substrates. This could be attributed to the nature of substrate and consequently the discrepancy between the dominance of microbial communities with regards to different substrate and at different phases of anaerobic digestion process. Knowledge of microbial communities involved would allow a rational substrate specific consortium design which will help to reduce consortium adaptation period and enhance the substrate utilisation resulting in improved efficacy of biogas process.

Keywords: amplicon sequencing, biomethane potential, community predominance, taxonomic analysis

Procedia PDF Downloads 499
9010 The Effect of Additive Acid on the Phytoremediation Efficiency

Authors: G. Hosseini, A. Sadighzadeh, M. Rahimnejad, N. Hosseini, Z. Jamalzadeh

Abstract:

Metal pollutants, especially heavy metals from anthropogenic sources such as metallurgical industries’ waste including mining, smelting, casting or production of nuclear fuel, including mining, concentrate production and uranium processing ends in the environment contamination (water and soil) and risk to human health around the facilities of this type of industrial activity. There are different methods that can be used to remove these contaminants from water and soil. These are very expensive and time-consuming. In this case, the people have been forced to leave the area and the decontamination is not done. For example, in the case of Chernobyl accident, an area of 30 km around the plant was emptied of human life. A very efficient and cost-effective method for decontamination of the soil and the water is phytoremediation. In this method, the plants preferentially native plants which are more adaptive to the regional climate are well used. In this study, three types of plants including Alfalfa, Sunflower and wheat were used to Barium decontamination. Alfalfa and Sunflower were not grown good enough in Saghand mine’s soil sample. This can be due to non-native origin of these plants. But, Wheat rise in Saghand Uranium Mine soil sample was satisfactory. In this study, we have investigated the effect of 4 types of acids inclusive nitric acid, oxalic acid, acetic acid and citric acid on the removal efficiency of Barium by Wheat. Our results indicate the increase of Barium absorption in the presence of citric acid in the soil. In this paper, we will present our research and laboratory results.

Keywords: phytoremediation, heavy metal, wheat, soil

Procedia PDF Downloads 296
9009 The Effects of Green Manure Returning on Properties and Fungal Communities in Vanadium/Titanium Magnet Tailings

Authors: Hai-Hong Gu, Yan-Jun Ai, Zheng Zhou

Abstract:

Vanadium and titanium are rare metals with superior properties and are important resources in aerospace, aviation, and military. The vanadium/titanium magnetite are mostly ultra-lean ores, and a large number of tailings has been produced in the exploitation process. The tailings are characterized by loose structure, poor nutrient, complex composition and high trace metal contents. Returning green manure has been shown to not only increase plant biomass and soil nutrients but also change the bioavailability of trace metals and the microbial community structure. Fungi play an important role in decomposing organic matter and increasing soil fertility, and the application of organic matter also affects the community structure of fungi. The effects of green manure plants, alfalfa (Medicago sativa L.), returned to the tailings in situ on community structure of fungi, nutrients and bioavailability of trace metals in vanadium/titanium magnetite tailings were investigated in a pot experiment. The results showed that the fungal community diversity and richness were increase after alfalfa green manure returned in situ. The dominant phyla of the fungal community were Ascomycota, Basidiomycota and Ciliophora, especially, the phyla Ciliophora was rare in ordinary soil, but had been found to be the dominant phyla in tailings. Meanwhile, the nutrient properties and various trace metals may shape the microbial communities by affecting the abundance of fungi. It was found that the plant growth was stimulated and the available N and organic C were significantly improved in the vanadium/titanium magnetite tailing with the long-term returning of alfalfa green manure. Moreover, the DTPA-TEA extractable Cd and Zn concentrations in the vanadium/titanium magnetite tailing were reduced by 7.72%~23.8% and 8.02%~24.4%, respectively, compared with those in the non-returning treatment. The above results suggest that the returning of alfalfa green manure could be a potential approach to improve fungal community structure and restore mine tailing ecosystem.

Keywords: fungal community, green manure returning, vanadium/titanium magnet tailings, trace metals

Procedia PDF Downloads 37
9008 Lead in The Soil-Plant System Following Aged Contamination from Ceramic Wastes

Authors: F. Pedron, M. Grifoni, G. Petruzzelli, M. Barbafieri, I. Rosellini, B. Pezzarossa

Abstract:

Lead contamination of agricultural land mainly vegetated with perennial ryegrass (Lolium perenne) has been investigated. The metal derived from the discharge of sludge from a ceramic industry in the past had used lead paints. The results showed very high values of lead concentration in many soil samples. In order to assess the lead soil contamination, a sequential extraction with H2O, KNO3, EDTA was performed, and the chemical forms of lead in the soil were evaluated. More than 70% of lead was in a potentially bioavailable form. Analysis of Lolium perenne showed elevated lead concentration. A Freundlich-like model was used to describe the transferability of the metal from the soil to the plant.

Keywords: bioavailability, Freundlich-like equation, sequential extraction, soil lead contamination

Procedia PDF Downloads 276
9007 Biological Treatment of a Mixture of Iodine-Containing Aromatic Compounds from Industrial Wastewaster

Authors: A. Elain, M. Le Fellic, A. Le Pemp, N. Hachet

Abstract:

Iodinated Compounds (IC) are widely detected contaminants in most aquatic environments including sewage treatment plant, surface water, ground water and even drinking water, up to the µg.L-1 range. As IC contribute in the adsorbable organic halides (AOX) level, their removal or dehalogenation is expected. We report here on the biodegradability of a mixture of IC from an industrial effluent using a microbial consortium adapted to grow on IC as well as the native microorganisms. Both aerobic and anaerobic treatments were studied during batch experiments in 500-mL flasks. The degree of mineralization and recovery of iodide were monitored by HPLC-UV, TOC analysis and potentiometric titration. Providing ethanol as an electron acceptor was found to stimulate anaerobic reductive deiodination of IC while sodium chloride even at high concentration (22 g.l-1) had no influence on the degradation rates nor on the microbial viability. Phylogenetic analysis of 16S RNA gene sequence (MicroSeq®) was applied to provide a better understanding of the degradative microbial community.

Keywords: iodinated compounds, biodegradability, deiodination, electron-accepting conditions, microbial consortium

Procedia PDF Downloads 292
9006 Dynamic Soil-Structure Interaction Analysis of Reinforced Concrete Buildings

Authors: Abdelhacine Gouasmia, Abdelhamid Belkhiri, Allaeddine Athmani

Abstract:

The objective of this paper is to evaluate the effects of soil-structure interaction (SSI) on the modal characteristics and on the dynamic response of current structures. The objective is on the overall behaviour of a real structure of five storeys reinforced concrete (R/C) building typically encountered in Algeria. Sensitivity studies are undertaken in order to study the effects of frequency content of the input motion, frequency of the soil-structure system, rigidity and depth of the soil layer on the dynamic response of such structures. This investigation indicated that the rigidity of the soil layer is the predominant factor in soil-structure interaction and its increases would definitely reduce the deformation in the R/C structure. On the other hand, increasing the period of the underlying soil will cause an increase in the lateral displacements at story levels and create irregularity in the distribution of story shears. Possible resonance between the frequency content of the input motion and soil could also play an important role in increasing the structural response.

Keywords: direct method, finite element method, foundation, R/C Frame, soil-structure interaction

Procedia PDF Downloads 607
9005 Fertigation Use in Agriculture and Biosorption of Residual Nitrogen by Soil Microorganisms

Authors: Irina Mikajlo, Jakub Elbl, Helena Dvořáčková, Antonín Kintl, Jindřich Kynický, Martin Brtnický, Jaroslav Záhora

Abstract:

Present work deals with the possible use of fertigation in agriculture and its impact on the availability of mineral nitrogen (Nmin) in topsoil and subsoil horizons. The aim of the present study is to demonstrate the effect of the organic matter presence in fertigation on microbial transformation and availability of mineral nitrogen forms. The main investigation reason is the potential use of pre-treated waste water, as a source of organic carbon (Corg) and residual nutrients (Nmin) for fertigation. Laboratory experiment has been conducted to demonstrate the effect of the arable land fertilization method on the Nmin availability in different depths of the soil with the usage of model experimental containers filled with soil from topsoil and podsoil horizons that were taken from the precise area. Tufted hairgrass (Deschampsia caespitosa) has been chosen as a model plant. The water source protection zone Brezova nad Svitavou has been a research area where significant underground reservoirs of drinking water of the highest quality are located. From the second half of the last century local sources of drinking water show nitrogenous compounds increase that get here almost only from arable lands. Therefore, an attention of the following text focuses on the fate of mineral nitrogen in the complex plant-soil. Research results show that the fertigation application with Corg in a combination with mineral fertilizer can reduce the amount of Nmin leached from topsoil horizon of agricultural soils. In addition, some plants biomass production reduce may occur.

Keywords: fertigation, fertilizers, mineral nitrogen, soil microorganisms

Procedia PDF Downloads 322
9004 Efficiency of Background Chlorine Residuals against Accidental Microbial Episode in Proto-Type Distribution Network (Rig) Using Central Composite Design (CCD)

Authors: Sajida Rasheed, Imran Hashmi, Luiza Campos, Qizhi Zhou, Kim Keu

Abstract:

A quadratic model (p ˂ 0.0001) was developed by using central composite design of 50 experimental runs (42 non-center + 8 center points) to assess efficiency of background chlorine residuals in combating accidental microbial episode in a prototype distribution network (DN) (rig). A known amount of background chlorine residuals were maintained in DN and a required number of bacteria, Escherichia coli K-12 strain were introduced by an injection port in the pipe loop system. Samples were taken at various time intervals at different pipe lengths. Spread plate count was performed to count bacterial number. The model developed was significant. With microbial concentration and time (p ˂ 0.0001), pipe length (p ˂ 0.022), background chlorine residuals (p ˂ 0.07) and time^2 (p ˂ 0.09) as significant factors. The ramp function of variables shows that at the microbial count of 10^6, at 0.76 L/min, and pipe length of 133 meters, a background residual chlorine 0.16 mg/L was enough for complete inactivation of microbial episode in approximately 18 minutes.

Keywords: central composite design (CCD), distribution network, Escherichia coli, residual chlorine

Procedia PDF Downloads 435
9003 Wastewater Treatment and Bio-Electricity Generation via Microbial Fuel Cell Technology Operating with Starch Proton Exchange Membrane

Authors: Livinus A. Obasi, Augustine N. Ajah

Abstract:

Biotechnology in recent times has tried to develop a mechanism whereby sustainable electricity can be generated by the activity of microorganisms on waste and renewable biomass (often regarded as “negative value”) in a device called microbial fuel cell, MFC. In this paper, we established how the biocatalytic activities of bacteria on organic matter (substrates) produced some electrons with the associated removal of some water pollution parameters; Biochemical oxygen demand (BOD), chemical oxygen demand (COD) to the tune of 77.2% and 88.3% respectively from a petrochemical sanitary wastewater. The electricity generation was possible by conditioning the bacteria to operate anaerobically in one chamber referred to as the anode while the electrons are transferred to the fully aerated counter chamber containing the cathode. Power densities ranging from 12.83 mW/m2 to 966.66 mW/m2 were achieved using a dual-chamber starch membrane MFC experimental set-up. The maximum power density obtained in this research shows an improvement in the use of low cost MFC set up to achieve power production. Also, the level of organic matter removal from the sanitary waste water by the operation of this device clearly demonstrates its potential benefit in achieving an improved benign environment. The beauty of the MFCs is their potential utility in areas lacking electrical infrastructures like in most developing countries.

Keywords: bioelectricity, COD, microbial fuel cell, sanitary wastewater, wheat starch

Procedia PDF Downloads 227
9002 Improving the Strength Characteristics of Soil Using Cotton Fibers

Authors: Bindhu Lal, Karnika Kochal

Abstract:

Clayey soil contains clay minerals with traces of metal oxides and organic matter, which exhibits properties like low drainage, high plasticity, and shrinkage. To overcome these issues, various soil reinforcement techniques are used to elevate the stiffness, water tightness, and bearing capacity of the soil. Such techniques include cementation, bituminization, freezing, fiber inclusion, geo-synthetics, nailing, etc. Reinforcement of soil with fibers has been a cost-effective solution to soil improvement problems. An experimental study was undertaken involving the inclusion of cotton waste fibers in clayey soil as reinforcement with different fiber contents (1%, 1.5%, 2%, and 2.5% by weight) and analyzing its effects on the unconfined compressive strength of the soil. Two categories of soil were taken, comprising of natural clay and clay mixed with 5% sodium bentonite by weight. The soil specimens were subjected to proctor compaction and unconfined compression tests. The validated outcome shows that fiber inclusion has a strikingly positive impact on the compressive strength and axial strain at failure of the soil. Based on the commendatory results procured, compressive strength was found to be directly proportional to the fiber content, with the effect being more pronounced at lower water content.

Keywords: bentonite clay, clay, cotton fibers, unconfined compressive strength

Procedia PDF Downloads 148
9001 Settlement of the Foundation on the Improved Soil: A Case Study

Authors: Morteza Karami, Soheila Dayani

Abstract:

Deep Soil Mixing (DSM) is a soil improvement technique that involves mechanically mixing the soil with a binder material to improve its strength, stiffness, and durability. This technique is typically used in geotechnical engineering applications where weak or unstable soil conditions exist, such as in building foundations, embankment support, or ground improvement projects. In this study, the settlement of the foundation on the improved soil using the wet DSM technique has been analyzed for a case study. Before DSM production, the initial soil mixture has been determined based on the laboratory tests and then, the proper mix designs have been optimized based on the pilot scale tests. The results show that the spacing and depth of the DSM columns depend on the soil properties, the intended loading conditions, and other factors such as the available space and equipment limitations. Moreover, monitoring instruments installed in the pilot area verify that the settlement of the foundation has been placed in an acceptable range to ensure that the soil mixture is providing the required strength and stiffness to support the structure or load. As an important result, if the DSM columns touch or penetrate into the stiff soil layer, the settlement of the foundation can be significantly decreased. Furthermore, the DSM columns should be allowed to cure sufficiently before placing any significant loads on the structure to prevent excessive deformation or settlement.

Keywords: deep soil mixing, soil mixture, settlement, instrumentation, curing age

Procedia PDF Downloads 55
9000 Numerical Modeling of a Retaining Wall in Soil Reinforced by Layers of Geogrids

Authors: M. Mellas, S. Baaziz, A. Mabrouki, D. Benmeddour

Abstract:

The reinforcement of massifs of backfill with horizontal layers of geosynthetics is an interesting economic solution, which ensures the stability of retaining walls. The mechanical behavior of reinforced soil by geosynthetic is complex, and requires studies and research to understand the mechanisms of rupture. The behavior of reinforcements in the soil and the behavior of the main elements of the system: reinforcement-wall-soil. The present study is interested in numerical modeling of a retaining wall in soil reinforced by horizontal layers of geogrids. This modeling makes use of the software FLAC3D. This work aims to analyze the effect of the length of the geogrid "L" where the soil massif is supporting a uniformly distributed surcharge "Q", taking into account the fixing elements rather than the layers of geogrids to the wall.

Keywords: retaining wall, geogrid, reinforced soil, numerical modeling, FLAC3D

Procedia PDF Downloads 455
8999 Disaggregation of Coarser Resolution Radiometer Derived Soil Moisture to Finer Scales

Authors: Gurjeet Singh, Rabindra K. Panda

Abstract:

Soil moisture is a key hydrologic state variable and is intrinsically linked to the Earth's water, climate and carbon cycles. On ecological point of view, the soil moisture is a fundamental natural resource providing the transpirable water for plants. Soil moisture varies both temporally and spatially due to spatiotemporal variation in rainfall, vegetation cover, soil properties and topography. Satellite derived soil moisture provides spatio-temporal extensive data. However, the spatial resolution of a typical satellite (L-band radiometry) is of the order of tens of kilometers, which is not good enough for developing efficient agricultural water management schemes at the field scale. In the present study, the soil moisture from radiometer data has been disaggregated using blending approach to achieve higher resolution soil moisture data. The radiometer estimates of soil moisture at a 40 km resolution have been disaggregated to 10 km, 5 km and 1 km resolutions. The disaggregated soil moisture was compared with the observed data, consisting of continuous sensor based soil moisture profile measurements, at three monitoring sites and extensive spatial near-surface soil moisture measurements, concurrent with satellite monitoring in the 500 km2 study watershed in the Eastern India. The estimated soil moisture status at different spatial scales can help in developing efficient agricultural water management schemes to increase the crop production and water use efficiency.

Keywords: disaggregation, eastern India, radiometers, soil moisture, water use efficiency

Procedia PDF Downloads 249