Search results for: Cluster Basis
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1406

Search results for: Cluster Basis

896 Motion Planning of SCARA Robots for Trajectory Tracking

Authors: Giovanni Incerti

Abstract:

The paper presents a method for a simple and immediate motion planning of a SCARA robot, whose end-effector has to move along a given trajectory; the calculation procedure requires the user to define in analytical form or by points the trajectory to be followed and to assign the curvilinear abscissa as function of the time. On the basis of the geometrical characteristics of the robot, a specifically developed program determines the motion laws of the actuators that enable the robot to generate the required movement; this software can be used in all industrial applications for which a SCARA robot has to be frequently reprogrammed, in order to generate various types of trajectories with different motion times.

Keywords: Motion planning, SCARA robot, trajectory tracking.

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895 A Codebook-based Redundancy Suppression Mechanism with Lifetime Prediction in Cluster-based WSN

Authors: Huan Chen, Bo-Chao Cheng, Chih-Chuan Cheng, Yi-Geng Chen, Yu Ling Chou

Abstract:

Wireless Sensor Network (WSN) comprises of sensor nodes which are designed to sense the environment, transmit sensed data back to the base station via multi-hop routing to reconstruct physical phenomena. Since physical phenomena exists significant overlaps between temporal redundancy and spatial redundancy, it is necessary to use Redundancy Suppression Algorithms (RSA) for sensor node to lower energy consumption by reducing the transmission of redundancy. A conventional algorithm of RSAs is threshold-based RSA, which sets threshold to suppress redundant data. Although many temporal and spatial RSAs are proposed, temporal-spatial RSA are seldom to be proposed because it is difficult to determine when to utilize temporal or spatial RSAs. In this paper, we proposed a novel temporal-spatial redundancy suppression algorithm, Codebookbase Redundancy Suppression Mechanism (CRSM). CRSM adopts vector quantization to generate a codebook, which is easily used to implement temporal-spatial RSA. CRSM not only achieves power saving and reliability for WSN, but also provides the predictability of network lifetime. Simulation result shows that the network lifetime of CRSM outperforms at least 23% of that of other RSAs.

Keywords: Redundancy Suppression Algorithm (RSA), Threshold-based RSA, Temporal RSA, Spatial RSA and Codebookbase Redundancy Suppression Mechanism (CRSM)

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894 A New Approach to Annotate the Text's of the Websites and Documents with a Quite Comprehensive Knowledge Base

Authors: Mohammad Yasrebi, Mehran Mohsenzadeh, Mashalla Abbasi-Dezfuli

Abstract:

Machine-understandable data when strongly interlinked constitutes the basis for the SemanticWeb. Annotating web documents is one of the major techniques for creating metadata on the Web. Annotating websites defines the containing data in a form which is suitable for interpretation by machines. In this paper, we present a new approach to annotate websites and documents by promoting the abstraction level of the annotation process to a conceptual level. By this means, we hope to solve some of the problems of the current annotation solutions.

Keywords: Knowledge base, ontology, semantic annotation, semantic web.

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893 Socio-Demographic Status and Arrack Drinking Patterns among Muslim, Hindu, Santal and Oraon Communities in Rasulpur Union,Bangladesh: A Cross-Cultural Perspective

Authors: Md. Emaj Uddin

Abstract:

Arrack is one of the forms of alcoholic beverage or liquor which is produced from palm or date juice and commonly consumed by the lower social class of all religious/ethnic communities in the north-western villages of Bangladesh. The purpose of the study was to compare arrack drinking patterns associated with socio-demographic status among the Muslim, Hindu, Santal, and Oraon communities in the Rasulpur union of Bangladesh. A total of 391 respondents (Muslim n-109, Hindu n-103, Santal n-89, Oraon n-90) selected by cluster random sampling were interviewed by ADP (Arrack Drinking Pattern) questionnaire. The results of Pearson Chi-Squire test revealed that arrack drinking patterns were significantly differed among the Muslim, Hindu, Santal, and Oraon communities- drinkers. In addition, the results of Spearman-s bivariate correlation coefficients also revealed that sociodemographic characteristics of the communities- drinkers were the significantly positive and negative associations with the arrack drinking patterns in the Rasulpur union, Bangladesh. The study suggests that further cross-cultural researches should be conducted on the consequences of arrack drinking patterns on the communities- drinkers.

Keywords: Arrack Drinking Patterns, Bangladesh, Community, Cross-Cultural Comparison, Socio-Demographic Status.

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892 Three-Dimensional Modeling of a Twisted-Blade Darrieus Vertical-Axis Wind Turbine

Authors: Three-Dimensional Modeling of a Twisted-Blade Darrieus Vertical-Axis Wind Turbine

Abstract:

A complete CAD procedure to model a twisted-bladed vertical-axis wind turbine (VAWT) is presented with the aim of determining some practical guidelines to be used for the generation of an easily-meshable CAD geometry to be adopted as the basis of both CFD and FEM numerical simulations.

Keywords: Vertical-axis wind turbine (VAWT), twisted blade, CAD, 3D modeling.

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891 Microseismicity of the Tehran Region Based on Three Seismic Networks

Authors: Jamileh Vasheghani Farahani

Abstract:

The main purpose of this research is to show the current active faults and active tectonic of the area by three seismic networks in Tehran region: 1-Tehran Disaster Mitigation and Management Organization (TDMMO), 2-Broadband Iranian National Seismic Network Center (BIN), 3-Iranian Seismological Center (IRSC). In this study, we analyzed microearthquakes happened in Tehran city and its surroundings using the Tehran networks from 1996 to 2015. We found some active faults and trends in the region. There is a 200-year history of historical earthquakes in Tehran. Historical and instrumental seismicity show that the east of Tehran is more active than the west. The Mosha fault in the North of Tehran is one of the active faults of the central Alborz. Moreover, other major faults in the region are Kahrizak, Eyvanakey, Parchin and North Tehran faults. An important seismicity region is an intersection of the Mosha and North Tehran fault systems (Kalan village in Lavasan). This region shows a cluster of microearthquakes. According to the historical and microseismic events analyzed in this research, there is a seismic gap in SE of Tehran. The empirical relationship is used to assess the Mmax based on the rupture length. There is a probability of occurrence of a strong motion of 7.0 to 7.5 magnitudes in the region (based on the assessed capability of the major faults such as Parchin and Eyvanekey faults and historical earthquakes).

Keywords: Iran, major faults, microseismicity, Tehran.

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890 On the Efficiency of Five Step Approximation Method for the Solution of General Third Order Ordinary Differential Equations

Authors: N. M. Kamoh, M. C. Soomiyol

Abstract:

In this work, a five step continuous method for the solution of third order ordinary differential equations was developed in block form using collocation and interpolation techniques of the shifted Legendre polynomial basis function. The method was found to be zero-stable, consistent and convergent. The application of the method in solving third order initial value problem of ordinary differential equations revealed that the method compared favorably with existing methods.

Keywords: Shifted Legendre polynomials, third order block method, discrete method, convergent.

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889 Effects of the Purpose Expropriation of Land Consolidation to Landholding

Authors: Turgut Ayten, Tayfun Çay

Abstract:

In the current expropriation of Turkey, the state acquires necessary lands for its investment without permission of the owners and not searching for alternative solutions, so it is determined that neither processor nor processed is not happy. In this study, interactions of enterprises in Turkey are analysed in case the necessary land for public investments are acquired by expropriation purposed land consolidation. Legal basis, positive and negative sides, financial effects to enterprises of this method is evaluated according to Konya Kadınhanı, Kolukısa avenue which is on the Konya-Ankara High-Speed Train Route.

Keywords: Land consolidation, expropriation purposed land consolidation, sustainable rural development, cost.

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888 Modeling of Processes Running in Radical Clusters Formed by Ionizing Radiation with the Help of Continuous Petri Nets and Oxygen Effect

Authors: J. Barilla, M. Lokajíček, H. Pisaková, P. Simr

Abstract:

The final biological effect of ionizing particles may be influenced strongly by some chemical substances present in cells mainly in the case of low-LET radiation. The influence of oxygen may by particularly important because oxygen is always present in living cells. The corresponding processes are then running mainly in the chemical stage of radiobiological mechanism.

The radical clusters formed by densely ionizing ends of primary or secondary charged particles are mainly responsible for final biological effect. The damage effect depends then on radical concentration at a time when the cluster meets a DNA molecule. It may be strongly influenced by oxygen present in a cell as oxygen may act in different directions: at small concentration of it the interaction with hydrogen radicals prevails while at higher concentrations additional efficient oxygen radicals may be formed.

The basic radical concentration in individual clusters diminishes, which is influenced by two parallel processes: chemical reactions and diffusion of corresponding clusters. The given simultaneous evolution may be modeled and analyzed well with the help of Continuous Petri nets. The influence of other substances present in cells during irradiation may be studied, too. Some results concerning the impact of oxygen content will be presented.

Keywords: DSB formation, chemical stage, Petri nets, radiobiological mechanism.

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887 Discovering Complex Regularities: from Tree to Semi-Lattice Classifications

Authors: A. Faro, D. Giordano, F. Maiorana

Abstract:

Data mining uses a variety of techniques each of which is useful for some particular task. It is important to have a deep understanding of each technique and be able to perform sophisticated analysis. In this article we describe a tool built to simulate a variation of the Kohonen network to perform unsupervised clustering and support the entire data mining process up to results visualization. A graphical representation helps the user to find out a strategy to optimize classification by adding, moving or delete a neuron in order to change the number of classes. The tool is able to automatically suggest a strategy to optimize the number of classes optimization, but also support both tree classifications and semi-lattice organizations of the classes to give to the users the possibility of passing from one class to the ones with which it has some aspects in common. Examples of using tree and semi-lattice classifications are given to illustrate advantages and problems. The tool is applied to classify macroeconomic data that report the most developed countries- import and export. It is possible to classify the countries based on their economic behaviour and use the tool to characterize the commercial behaviour of a country in a selected class from the analysis of positive and negative features that contribute to classes formation. Possible interrelationships between the classes and their meaning are also discussed.

Keywords: Unsupervised classification, Kohonen networks, macroeconomics, Visual data mining, Cluster interpretation.

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886 Effect of Soil Corrosion in Failures of Buried Gas Pipelines

Authors: Saima Ali, Pathamanathan Rajeev, Imteaz A. Monzur

Abstract:

In this paper, a brief review of the corrosion mechanism in buried pipe and modes of failure is provided together with the available corrosion models. Moreover, the sensitivity analysis is performed to understand the influence of corrosion model parameters on the remaining life estimation. Further, the probabilistic analysis is performed to propagate the uncertainty in the corrosion model on the estimation of the renaming life of the pipe. Finally, the comparison among the corrosion models on the basis of the remaining life estimation will be provided to improve the renewal plan.

Keywords: Corrosion, pit depth, sensitivity analysis, exposure period.

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885 Evaluation of a Surrogate Based Method for Global Optimization

Authors: David Lindström

Abstract:

We evaluate the performance of a numerical method for global optimization of expensive functions. The method is using a response surface to guide the search for the global optimum. This metamodel could be based on radial basis functions, kriging, or a combination of different models. We discuss how to set the cyclic parameters of the optimization method to get a balance between local and global search. We also discuss the eventual problem with Runge oscillations in the response surface.

Keywords: Expensive function, infill sampling criterion, kriging, global optimization, response surface, Runge phenomenon.

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884 Compensation–Based Current Decomposition

Authors: Mihaela Popescu, Alexandru Bitoleanu, Mircea Dobriceanu

Abstract:

This paper deals with the current space-vector decomposition in three-phase, three-wire systems on the basis of some case studies. We propose four components of the current spacevector in terms of DC and AC components of the instantaneous active and reactive powers. The term of supplementary useless current vector is also pointed out. The analysis shows that the current decomposition which respects the definition of the instantaneous apparent power vector is useful for compensation reasons only if the supply voltages are sinusoidal. A modified definition of the components of the current is proposed for the operation under nonsinusoidal voltage conditions.

Keywords: Active current, Active filtering, p–q theory, Reactive current.

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883 Problems of Measuring Effectiveness of Innovation Performance

Authors: Aziza S. Zhuparova

Abstract:

The innovation performance of nations has been repeatedly measured in the literature. We argue that while the literature offers many suggestions, their theoretical foundation is often weak and the underlying assumptions are rarely discussed. In this paper, we systematize various mechanisms by which spatial units influence nation and firms' innovation activities. On the basis of this, common innovation performance measures and analyses are discussed and evaluated. It is concluded that there is no general best way of measuring the innovation performance of spatial units. In fact, the most interesting insights can be obtained using a multitude of different approaches at the same time.

Keywords: Innovation performance, firms effectiveness of innovation, national innovativeness, innovation effectiveness, national innovation activity's measurement

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882 Comparison between LQR and ANN Active Anti-Roll Control of a Single Unit Heavy Vehicle

Authors: Babesse Saad, Ameddah Djameleddine

Abstract:

In this paper, a learning algorithm using neuronal networks to improve the roll stability and prevent the rollover in a single unit heavy vehicle is proposed. First, LQR control to keep balanced normalized rollovers, between front and rear axles, below the unity, then a data collected from this controller is used as a training basis of a neuronal regulator. The ANN controller is thereafter applied for the nonlinear side force model, and gives satisfactory results than the LQR one.

Keywords: Rollover, single unit heavy vehicle, neural networks, nonlinear side force.

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881 Architecture Exception Governance

Authors: Ondruska Marek

Abstract:

The article presents the whole model of IS/IT architecture exception governance. As first, the assumptions of presented model are set. As next, there is defined a generic governance model that serves as a basis for the architecture exception governance. The architecture exception definition and its attributes follow. The model respects well known approaches to the area that are described in the text, but it adopts higher granularity in description and expands the process view with all the next necessary governance components as roles, principles and policies, tools to enable the implementation of the model into organizations. The architecture exception process is decomposed into a set of processes related to the architecture exception lifecycle consisting of set of phases and architecture exception states. Finally, there is information about my future research related to this area.

Keywords: Architecture, dispensation, exception, governance, model

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880 Framework for the Modeling of the Supply Chain Collaborative Planning Process

Authors: D. Pérez, M. M. E. Alemany

Abstract:

In this work, a framework to model the Supply Chain (SC) Collaborative Planning (CP) process is proposed. The main contributions of this framework concern 1) the presentation of the decision view, the most important one due to the characteristics of the process, jointly within the physical, organisation and information views, and 2) the simultaneous consideration of the spatial and temporal integration among the different supply chain decision centres. This framework provides the basis for a realistic and integrated perspective of the supply chain collaborative planning process and also the analytical modeling of each of its decisional activities.

Keywords: Collaborative Planning, Decision View, Distributed Decision-Making, Framework.

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879 A Bibliometric Assessment on Sustainability and Clustering

Authors: Fernanda M. Assef, Maria Teresinha A. Steiner, David Gabriel F. de Barros

Abstract:

Review researches are useful in terms of analysis of research problems. Between the types of review documents, we commonly find bibliometric studies. This type of application often helps the global visualization of a research problem and helps academics worldwide to understand the context of a research area better. In this document, a bibliometric view surrounding clustering techniques and sustainability problems is presented. The authors aimed at which issues mostly use clustering techniques and even which sustainability issue would be more impactful on today’s moment of research. During the bibliometric analysis, we found 10 different groups of research in clustering applications for sustainability issues: Energy; Environmental; Non-urban Planning; Sustainable Development; Sustainable Supply Chain; Transport; Urban Planning; Water; Waste Disposal; and, Others. Moreover, by analyzing the citations of each group, it was discovered that the Environmental group could be classified as the most impactful research cluster in the area mentioned. After the content analysis of each paper classified in the environmental group, it was found that the k-means technique is preferred for solving sustainability problems with clustering methods since it appeared the most amongst the documents. The authors finally conclude that a bibliometric assessment could help indicate a gap of researches on waste disposal – which was the group with the least amount of publications – and the most impactful research on environmental problems.

Keywords: Bibliometric assessment, clustering, sustainability, territorial partitioning.

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878 Some Physical Properties of Musk Lime (Citrus Microcarpa)

Authors: M.H.R.O. Abdullah, P.E. Ch'ng, N.A. Yunus

Abstract:

Some physical properties of musk lime (Citrus microcarpa) were determined in this study. The average moisture content (wet basis) of the fruit was found to be 85.10 (±0.72) %. The mean of length, width and thickness of the fruit was 26.36 (±0.97), 26.40 (±1.04) and 25.26 (±0.94) mm respectively. The average value for geometric mean diameter, sphericity, aspect ratio, mass, surface area, volume, true density, bulk density and porosity was 26.00 (±0.82) mm, 98.67 (±2.04) %, 100.23 (±3.28) %, 10.007 (±0.878) g, 2125.07 (±133.93) mm2, 8800.00 (±731.82) mm3, 1002.87 (±39.16) kgm-3, 501.70 (±22.58) kgm-3 and 49.89 (±3.15) % respectively. The coefficient of static friction on four types of structural surface was found to be varying from 0.238 (±0.025) for glass to 0.247 (±0.024) for steel surface.

Keywords: Musk lime, Citrus microcarpa, physical properties.

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877 Adaptation of Iterative Methods to Solve Fuzzy Mathematical Programming Problems

Authors: Ricardo C. Silva, Luiza A. P. Cantao, Akebo Yamakami

Abstract:

Based on the fuzzy set theory this work develops two adaptations of iterative methods that solve mathematical programming problems with uncertainties in the objective function and in the set of constraints. The first one uses the approach proposed by Zimmermann to fuzzy linear programming problems as a basis and the second one obtains cut levels and later maximizes the membership function of fuzzy decision making using the bound search method. We outline similarities between the two iterative methods studied. Selected examples from the literature are presented to validate the efficiency of the methods addressed.

Keywords: Fuzzy Theory, Nonlinear Optimization, Fuzzy Mathematics Programming.

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876 Effect of Composition on Work Hardening Coefficient of Bismuth-Lead Binary Alloy

Authors: K. A. Mistry, I. B. Patel, A. H. Prajapati

Abstract:

In the present work, the alloy of Bismuth-lead is prepared on the basis of percentage of molecular weight 9:1, 5:5 and 1:9 ratios and grown by Zone- Refining Technique under a vacuum atmosphere. The EDAX of these samples are done and the results are reported. Micro hardness test has been used as an alternative test for measuring material’s tensile properties. The effect of temperature and load on the hardness of the grown alloy has been studied. Further the comparative studies of work hardening coefficients are reported.

Keywords: EDAX, hardening coefficient, Micro hardness, Bi-Pb alloy.

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875 AGENTMAP: A Conceptual Meta-Model of Interacting Simulations

Authors: Thomas M. Prinz Wilhelm R. Rossak, Kai Gebhardt

Abstract:

A straightforward and intuitive combination of single simulations into an aggregated master-simulation is not trivial. There are lots of problems, which trigger-specific difficulties during the modeling and execution of such a simulation. In this paper we identify these problems and aim to solve them by mapping the task to the field of multi agent systems. The solution is a new meta-model named AGENTMAP, which is able to mitigate most of the problems and to support intuitive modeling at the same time. This meta-model will be introduced and explained on basis of an example from the e-commerce domain.

Keywords: Multi Agent System, Agent-based Simulation, Distributed Systems, Meta-models.

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874 Myanmar Character Recognition Using Eight Direction Chain Code Frequency Features

Authors: Kyi Pyar Zaw, Zin Mar Kyu

Abstract:

Character recognition is the process of converting a text image file into editable and searchable text file. Feature Extraction is the heart of any character recognition system. The character recognition rate may be low or high depending on the extracted features. In the proposed paper, 25 features for one character are used in character recognition. Basically, there are three steps of character recognition such as character segmentation, feature extraction and classification. In segmentation step, horizontal cropping method is used for line segmentation and vertical cropping method is used for character segmentation. In the Feature extraction step, features are extracted in two ways. The first way is that the 8 features are extracted from the entire input character using eight direction chain code frequency extraction. The second way is that the input character is divided into 16 blocks. For each block, although 8 feature values are obtained through eight-direction chain code frequency extraction method, we define the sum of these 8 feature values as a feature for one block. Therefore, 16 features are extracted from that 16 blocks in the second way. We use the number of holes feature to cluster the similar characters. We can recognize the almost Myanmar common characters with various font sizes by using these features. All these 25 features are used in both training part and testing part. In the classification step, the characters are classified by matching the all features of input character with already trained features of characters.

Keywords: Chain code frequency, character recognition, feature extraction, features matching, segmentation.

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873 Comparison of Newton Raphson and Gauss Seidel Methods for Power Flow Analysis

Authors: H. Abaali, T. Talbi, R.Skouri

Abstract:

This paper presents a comparative study of the Gauss Seidel and Newton-Raphson polar coordinates methods for power flow analysis. The effectiveness of these methods are evaluated and tested through a different IEEE bus test system on the basis of number of iteration, computational time, tolerance value and convergence.

Keywords: Convergence time, Gauss-Seidel Method, Newton-Raphson Method, number of iteration, power flow analysis.

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872 Simulation Programs to Education of Crisis Management Members

Authors: Jiri Barta

Abstract:

This paper deals with a simulation programs and technologies using in the educational process for members of the crisis management. Risk analysis, simulation, preparation and planning are among the main activities of workers of crisis management. Made correctly simulation of emergency defines the extent of the danger. On this basis, it is possible to effectively prepare and plan measures to minimize damage. The paper is focused on simulation programs that are trained at the University of Defence. Implementation of the outputs from simulation programs in decision-making processes of crisis staffs is one of the main tasks of the research project.

Keywords: Crisis Management, Continuity, Critical Infrastructure, Dangerous substance, Education, Flood, Simulation Programs.

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871 Enhanced Bidirectional Selection Sort

Authors: Jyoti Dua

Abstract:

An algorithm is a well-defined procedure that takes some input in the form of some values, processes them and gives the desired output. It forms the basis of many other algorithms such as searching, pattern matching, digital filters etc., and other applications have been found in database systems, data statistics and processing, data communications and pattern matching. This paper introduces algorithmic “Enhanced Bidirectional Selection” sort which is bidirectional, stable. It is said to be bidirectional as it selects two values smallest from the front and largest from the rear and assigns them to their appropriate locations thus reducing the number of passes by half the total number of elements as compared to selection sort.

Keywords: Bubble sort, cocktail sort, selection sort, heap sort.

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870 Sensitive Detection of Nano-Scale Vibrations by the Metal-Coated Fiber Tip at the Liquid-Air Interface

Authors: A. J. Babajanyan, T. A. Abrahamyan, H. A. Minasyan, Kh. V. Nerkararyan

Abstract:

Optical radiation emitted from a metal-coated fiber tip apex at liquid-air interface was measured. The intensity of the output radiation was strongly depend on the relative position of the tip to a liquid-air interface and varied with surface fluctuations. This phenomenon permits in-situ real-time investigation of nano-metric vibrations of the liquid surface and provides a basis for development of various origin ultrasensitive vibration detecting sensors. The described method can be used for detection of week seismic vibrations.

Keywords: Fiber-tip, Liquid-air interface, Nano vibration, Opto-mechanical sensor.

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869 Micropower Composite Nanomaterials Based on Porous Silicon for Renewable Energy Sources

Authors: Alexey P. Antropov, Alexander V. Ragutkin, Nicolay A. Yashtulov

Abstract:

The original controlled technology for power active nanocomposite membrane-electrode assembly engineering on the basis of porous silicon is presented. The functional nanocomposites were studied by electron microscopy and cyclic voltammetry methods. The application possibility of the obtained nanocomposites as high performance renewable energy sources for micro-power electronic devices is demonstrated.

Keywords: Cyclic voltammetry, electron microscopy, nanotechnology, platinum-palladium nanocomposites, porous silicon, power activity, renewable energy sources.

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868 A New Bound on the Average Information Ratio of Perfect Secret-Sharing Schemes for Access Structures Based On Bipartite Graphs of Larger Girth

Authors: Hui-Chuan Lu

Abstract:

In a perfect secret-sharing scheme, a dealer distributes a secret among a set of participants in such a way that only qualified subsets of participants can recover the secret and the joint share of the participants in any unqualified subset is statistically independent of the secret. The access structure of the scheme refers to the collection of all qualified subsets. In a graph-based access structures, each vertex of a graph G represents a participant and each edge of G represents a minimal qualified subset. The average information ratio of a perfect secret-sharing scheme realizing a given access structure is the ratio of the average length of the shares given to the participants to the length of the secret. The infimum of the average information ratio of all possible perfect secret-sharing schemes realizing an access structure is called the optimal average information ratio of that access structure. We study the optimal average information ratio of the access structures based on bipartite graphs. Based on some previous results, we give a bound on the optimal average information ratio for all bipartite graphs of girth at least six. This bound is the best possible for some classes of bipartite graphs using our approach.

Keywords: Secret-sharing scheme, average information ratio, star covering, deduction, core cluster.

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867 Quantification of Peptides based on Isotope Dilution Surface Enhanced Raman Scattering

Authors: F. Yaghobian, R. Stosch, B. Güttler

Abstract:

This study aims to demonstrate the quantification of peptides based on isotope dilution surface enhanced Raman scattering (IDSERS). SERS spectra of phenylalanine (Phe), leucine (Leu) and two peptide sequences TGQIFK (T13) and YSFLQNPQTSLCFSESIPTPSNR (T6) as part of the 22-kDa human growth hormone (hGH) were obtained on Ag-nanoparticle covered substrates. On the basis of the dominant Phe and Leu vibrational modes, precise partial least squares (PLS) prediction models were built enabling the determination of unknown T13 and T6 concentrations. Detection of hGH in its physiological concentration in order to investigate the possibility of protein quantification has been achieved.

Keywords: Surface Enhanced Raman Scattering, Quantification, Peptides.

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