Search results for: Coalescence; electrolytes; liquid-liquid separation; high viscosity; mixer- settler.
5849 Spectral Analysis of Speech: A New Technique
Authors: Neeta Awasthy, J.P.Saini, D.S.Chauhan
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ICA which is generally used for blind source separation problem has been tested for feature extraction in Speech recognition system to replace the phoneme based approach of MFCC. Applying the Cepstral coefficients generated to ICA as preprocessing has developed a new signal processing approach. This gives much better results against MFCC and ICA separately, both for word and speaker recognition. The mixing matrix A is different before and after MFCC as expected. As Mel is a nonlinear scale. However, cepstrals generated from Linear Predictive Coefficient being independent prove to be the right candidate for ICA. Matlab is the tool used for all comparisons. The database used is samples of ISOLET.Keywords: Cepstral Coefficient, Distance measures, Independent Component Analysis, Linear Predictive Coefficients.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19575848 Effect of Adverse Pressure Gradient on a Fluctuating Velocity over the Co-Flow Jet Airfoil
Authors: Morteza Mirhosseini, Amir B. Khoshnevis
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The boundary layer separation and new active flow control of a NACA 0025 airfoil were studied experimentally. This new flow control is sometimes known as a co-flow jet (cfj) airfoil. This paper presents the fluctuating velocity in a wall jet over the co-flow jet airfoil subjected to an adverse pressure gradient and a curved surface. In these results, the fluctuating velocity at the inner part increasing by increased the angle of attack up to 12o and this has due to the jet energized, while the angle of attack 20o has different. The airfoil cord based Reynolds number has 105.Keywords: Adverse pressure gradient, fluctuating velocity, wall jet, co-flow jet airfoil.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14695847 Effect of Rice Husk Ash on Strength and Durability of High Strength High Performance Concrete
Authors: H. B. Mahmud, Syamsul Bahri, Y. W. Yee, Y. T. Yeap
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This paper reports the strength and durability properties of high strength high performance concrete incorporating rice husk ash (RHA) having high silica, low carbon content and appropriate fineness. In this study concrete containing 10%, 15% and 20% RHA as cement replacement and water to binder ratio of 0.25 were investigated. The results show that increasing amount of RHA increases the dosage of superplasticizer to maintain similar workability. Partial replacement of cement with RHA did not increase the early age compressive strength of concrete. However, concrete containing RHA showed higher compressive strength at later ages. The results showed that compressive strength of concrete in the 90-115 MPa range can be obtained at 28 curing days and the durability properties of RHA concrete performed better than that of control concrete. The water absorption of concrete incorporating 15% RHA exhibited the lowest value. The porosity of concrete is consistent with water absorption whereby higher replacement of RHA decreased the porosity of concrete. There is a positive correlation between reducing porosity and increasing compressive strength of high strength high performance concrete. The results also indicate that up to 20% of RHA incorporation could be advantageously blended with cement without adversely affecting the strength and durability properties of concrete.
Keywords: Compressive strength, durability, high performance concrete, rice husk ash.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 27345846 Assessment of Ultra-High Cycle Fatigue Behavior of EN-GJL-250 Cast Iron Using Ultrasonic Fatigue Testing Machine
Authors: Saeedeh Bakhtiari, Johannes Depessemier, Stijn Hertelé, Wim De Waele
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High cycle fatigue comprising up to 107 load cycles has been the subject of many studies, and the behavior of many materials was recorded adequately in this regime. However, many applications involve larger numbers of load cycles during the lifetime of machine components. In this ultra-high cycle regime, other failure mechanisms play, and the concept of a fatigue endurance limit (assumed for materials such as steel) is often an oversimplification of reality. When machine component design demands a high geometrical complexity, cast iron grades become interesting candidate materials. Grey cast iron is known for its low cost, high compressive strength, and good damping properties. However, the ultra-high cycle fatigue behavior of cast iron is poorly documented. The current work focuses on the ultra-high cycle fatigue behavior of EN-GJL-250 (GG25) grey cast iron by developing an ultrasonic (20 kHz) fatigue testing system. Moreover, the testing machine is instrumented to measure the temperature and the displacement of the specimen, and to control the temperature. The high resonance frequency allowed to assess the behavior of the cast iron of interest within a matter of days for ultra-high numbers of cycles, and repeat the tests to quantify the natural scatter in fatigue resistance.Keywords: GG25, cast iron, ultra-high cycle fatigue, ultrasonic test.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 8025845 The Effects of Peristalsis on Dispersion of a Micropolar Fluid in the Presence of Magnetic Field
Authors: Habtu Alemayehu, G. Radhakrishnamacharya
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The paper presents an analytical solution for dispersion of a solute in the peristaltic motion of a micropolar fluid in the presence of magnetic field and both homogeneous and heterogeneous chemical reactions. The average effective dispersion coefficient has been found using Taylor-s limiting condition under long wavelength approximation. The effects of various relevant parameters on the average coefficient of dispersion have been studied. The average effective dispersion coefficient increases with amplitude ratio, cross viscosity coefficient and heterogeneous chemical reaction rate parameter. But it decreases with magnetic field parameter and homogeneous chemical reaction rate parameter. It can be noted that the presence of peristalsis enhances dispersion of a solute.Keywords: Peristalsis, Dispersion, Chemical reaction, Magneticfield, Micropolar fluid
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 17185844 U.S. Supreme Court Justices and Partisanship: Support for the President and Solicitor General
Authors: James Meernik, Joseph Ignagni, Rebecca Deen
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This paper analyzes the extent to which the justices of the U.S. Supreme Court cast votes that support the positions of the president, or more generally the Executive Branch. Can presidents count on such deference from those justices they nominate or those whom are nominated by other presidents of the same party? Or, do the justices demonstrate judicial independence and impartiality such that they are not so predisposed to vote in favor of arguments of their nominating president-s party? The results suggest that while in general the justices do not exhibit any marked tendency to partisan support of presidents, more recent and conservative Supreme Court justices are significantly more likely to support Republican presidents.Keywords: Separation of Powers, Solicitor General, U.S. President
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14185843 State of Charge Estimator Based On High-Gain Observer for Lithium-Ion Batteries
Authors: Jaeho Han, Moonjung Kim, Won-Ho Kim, Chang-Ho Hyun
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This paper introduces a high-gain observer based state of charge(SOC) estimator for lithium-Ion batteries. The proposed SOC estimator has a high-gain observer(HGO) structure. The HGO scheme enhances the transient response speed and diminishes the effect of uncertainties. Furthermore, it guarantees that the output feedback controller recovers the performance of the state feedback controller when the observer gain is sufficiently high. In order to show the effectiveness of the proposed method, the linear RC battery model in ADVISOR is used. The performance of the proposed method is compared with that of the conventional linear observer(CLO) and some simulation result is given.
Keywords: SOC, high-gain, observer, uncertainties, robust
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15525842 Simulation Study on the Indoor Thermal Comfort with Insulation on Interior Structural Components of Super High-Rise Residences
Authors: Y. Wang, H. Fukuda, A. Ozaki, H. Sato
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In this study, we discussed the effects on the thermal comfort of super high-rise residences that how effected by the high thermal capacity structural components. We considered different building orientations, structures, and insulation methods. We used the dynamic simulation software THERB (simulation of the thermal environment of residential buildings). It can estimate the temperature, humidity, sensible temperature, and heating/cooling load for multiple buildings. In the past studies, we examined the impact of air-conditioning loads (hereinafter referred to as AC loads) on the interior structural parts and the AC-usage patterns of super-high-rise residences. Super-high-rise residences have more structural components such as pillars and beams than do ordinary apartment buildings. The skeleton is generally made of concrete and steel, which have high thermal-storage capacities. The thermal-storage capacity of super-high-rise residences is considered to have a larger impact on the AC load and thermal comfort than that of ordinary residences. We show that the AC load of super-high-rise units would be reduced by installing insulation on the surfaces of interior walls that are not usually insulated in Japan.Keywords: High-rise Residences, AC Load, Thermal Comfort, Thermal Storage, Insulation Patterns
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15435841 Fuzzy Controlled Hydraulic Excavator with Model Parameter Uncertainty
Authors: Ganesh Kothapalli, Mohammed Y. Hassan
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The hydraulic actuated excavator, being a non-linear mobile machine, encounters many uncertainties. There are uncertainties in the hydraulic system in addition to the uncertain nature of the load. The simulation results obtained in this study show that there is a need for intelligent control of such machines and in particular interval type-2 fuzzy controller is most suitable for minimizing the position error of a typical excavator-s bucket under load variations. We consider the model parameter uncertainties such as hydraulic fluid leakage and friction. These are uncertainties which also depend up on the temperature and alter bulk modulus and viscosity of the hydraulic fluid. Such uncertainties together with the load variations cause chattering of the bucket position. The interval type-2 fuzzy controller effectively eliminates the chattering and manages to control the end-effecter (bucket) position with positional error in the order of few millimeters.Keywords: excavator, fuzzy control, hydraulics, mining, type-2
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16435840 High-Rises and Urban Design: The Reasons for Unsuccessful Placemaking with Residential High-Rises in England
Authors: E. Kalcheva, A. Taki, Y. Hadi
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High-rises and placemaking is an understudied combination which receives more and more interest with the proliferation of this typology in many British cities. The reason for studying three major cities in England: London, Birmingham and Manchester, is to learn from the latest advances in urban design in well-developed and prominent urban environment. The analysis of several high-rise sites reveals the weaknesses in urban design of contemporary British cities and presents an opportunity to study from the implemented examples. Therefore, the purpose of this research is to analyze design approaches towards creating a sustainable and varied urban environment when high-rises are involved. The research questions raised by the study are: what is the quality of high-rises and their surroundings; what facilities and features are deployed in the research area; what is the role of the high-rise buildings in the placemaking process; what urban design principles are applicable in this context. The methodology utilizes observation of the researched area by structured questions, developed by the author to evaluate the outdoor qualities of the high-rise surroundings. In this context, the paper argues that the quality of the public realm around the high-rises is quite low, missing basic but vital elements such as plazas, public art, and seating, along with landscaping and pocket parks. There is lack of coherence, the rhythm of the streets is often disrupted, and even though the high-rises are very aesthetically appealing, they fail to create a sense of place on their own. The implications of the study are that future planning can take into consideration the critique in this article and provide more opportunities for urban design interventions around high-rise buildings in the British cities.
Keywords: High-rises, placemaking, urban design, townscape.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20285839 Fast High Voltage Solid State Switch Using Insulated Gate Bipolar Transistor for Discharge-Pumped Lasers
Authors: Nur Syarafina Binti Othman, Tsubasa Jindo, Makato Yamada, Miho Tsuyama, Hitoshi Nakano
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A novel method to produce a fast high voltage solid states switch using Insulated Gate Bipolar Transistors (IGBTs) is presented for discharge-pumped gas lasers. The IGBTs are connected in series to achieve a high voltage rating. An avalanche transistor is used as the gate driver. The fast pulse generated by the avalanche transistor quickly charges the large input capacitance of the IGBT, resulting in a switch out of a fast high-voltage pulse. The switching characteristic of fast-high voltage solid state switch has been estimated in the multi-stage series-connected IGBT with the applied voltage of several tens of kV. Electrical circuit diagram and the mythology of fast-high voltage solid state switch as well as experimental results obtained are presented.
Keywords: High voltage, IGBT, Solid states switch.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 59125838 Hybrid Quasi-Steady Thermal Lattice Boltzmann Model for Studying the Behavior of Oil in Water Emulsions Used in Machining Tool Cooling and Lubrication
Authors: W. Hasan, H. Farhat, A. Alhilo, L. Tamimi
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Oil in water (O/W) emulsions are utilized extensively for cooling and lubricating cutting tools during parts machining. A robust Lattice Boltzmann (LBM) thermal-surfactants model, which provides a useful platform for exploring complex emulsions’ characteristics under variety of flow conditions, is used here for the study of the fluid behavior during conventional tools cooling. The transient thermal capabilities of the model are employed for simulating the effects of the flow conditions of O/W emulsions on the cooling of cutting tools. The model results show that the temperature outcome is slightly affected by reversing the direction of upper plate (workpiece). On the other hand, an important increase in effective viscosity is seen which supports better lubrication during the work.
Keywords: Hybrid lattice Boltzmann method, Gunstensen model, thermal, surfactant-covered droplet, Marangoni stress.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 7835837 A Silicon Controlled Rectifier-Based ESD Protection Circuit with High Holding Voltage and High Robustness Characteristics
Authors: Kyoung-il Do, Byung-seok Lee, Hee-guk Chae, Jeong-yun Seo Yong-seo Koo
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In this paper, a Silicon Controlled Rectifier (SCR)-based Electrostatic Discharge (ESD) protection circuit with high holding voltage and high robustness characteristics is proposed. Unlike conventional SCR, the proposed circuit has low trigger voltage and high holding voltage and provides effective ESD protection with latch-up immunity. In addition, the TCAD simulation results show that the proposed circuit has better electrical characteristics than the conventional SCR. A stack technology was used for voltage-specific applications. Consequentially, the proposed circuit has a trigger voltage of 17.60 V and a holding voltage of 3.64 V.Keywords: ESD, SCR, latch-up, power clamp, holding voltage.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 8295836 A Low Voltage High Performance Self Cascode Current Mirror
Authors: Jasdeep Kaur, Nupur Prakash, S. S. Rajput
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A current mirror (CM) based on self cascode MOSFETs low voltage analog and mixed mode structures has been proposed. The proposed CM has high output impedance and can operate at 0.5 V. P-Spice simulations confirm the high performance of this CM with a bandwidth of 6.0 GHz at input current of 100 μA.Keywords: Current Mirrors, Composite Cascode Structure, Current Source/Sink
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 43805835 Investigation and Perfection of Centrifugal Compressor Stages by CFD Methods
Authors: Y. Galerkin, L. Marenina
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Stator elements «Vane diffuser + crossover + return channel» of stages with different specific speed were investigated by CFD calculations. The regime parameter was introduced to present efficiency and loss coefficient performance of all elements together. Flow structure demonstrated advantages and disadvantages of design. Flow separation in crossovers was eliminated by its shape modification. Efficiency increased visibly. Calculated CFD performances are in acceptable correlation with predicted ones by engineering design method. The information obtained is useful for design method better calibration.
Keywords: Vane diffuser, return channel, crossover, efficiency, loss coefficient, inlet flow angle.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 21905834 Analysis on Urban Form and Evolution Mechanism of High-Density City: Case Study of Hong Kong
Authors: Yuan Zhang
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Along with large population and great demands for urban development, Hong Kong serves as a typical high-density city with multiple altitudes, advanced three-dimensional traffic system, rich city open space, etc. This paper contributes to analyzing its complex urban form and evolution mechanism from three aspects of view, separately as time, space and buildings. Taking both horizontal and vertical dimension into consideration, this paper provides a perspective to explore the fascinating process of growing and space folding in the urban form of high-density city, also as a research reference for related high-density urban design.
Keywords: Evolution mechanism, high-density city, Hong Kong, urban form.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12185833 Excitation Experiments of a Cone Loudspeaker and Vibration-Acoustic Analysis Using FEM
Authors: Y. Hu, X. Zhao, T. Yamaguchi, M. Sasajima, Y. Koike
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To focus on the vibration mode of a cone loudspeaker, which acts as an electroacoustic transducer, excitation experiments were performed using two types of loudspeaker units: one employing an impulse hammer and the other a sweep signal. The on-axis sound pressure frequency properties of the loudspeaker were evaluated, and the characteristic properties of the loudspeakers were successfully determined in both excitation experiments. Moreover, under conditions identical to the experiment conditions, a coupled analysis of the vibration-acoustics of the cone loudspeaker was performed using an acoustic analysis software program that considers the impact of damping caused by air viscosity. The result of sound pressure frequency properties with the numerical analysis are the most closely match that measured in the excitation experiments over a wide range of frequency bands.Keywords: Anechoic room, finite element method, impulse hammer, loudspeaker, reverberation room, sweep signal.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 17985832 Experimental Studies on Multiphase Flow in Porous Media and Pore Wettability
Authors: Xingxun Li, Xianfeng Fan
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Multiphase flow transport in porous medium is very common and significant in science and engineering applications. For example, in CO2 Storage and Enhanced Oil Recovery processes, CO2 has to be delivered to the pore spaces in reservoirs and aquifers. CO2 storage and enhance oil recovery are actually displacement processes, in which oil or water is displaced by CO2. This displacement is controlled by pore size, chemical and physical properties of pore surfaces and fluids, and also pore wettability. In this study, a technique was developed to measure the pressure profile for driving gas/liquid to displace water in pores. Through this pressure profile, the impact of pore size on the multiphase flow transport and displacement can be analyzed. The other rig developed can be used to measure the static and dynamic pore wettability and investigate the effects of pore size, surface tension, viscosity and chemical structure of liquids on pore wettability.
Keywords: Enhanced oil recovery, Multiphase flow, Pore size, Pore wettability
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 23275831 Preparation and Physical Assessment of Portland Cement Base Composites Containing Nano Particles
Authors: Amir Mahmoudi
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In this research the effects of adding silica and alumina nanoparticles on flow ability and compressive strength of cementitious composites based on Portland cement were investigated. In the first stage, the rheological behavior of different samples containing nanosilica, nanoalumina and polypropylene, polyvinyl alcohol and polyethylene fibers were evaluated. With increasing of nanoparticles in fresh samples, the slump flow diameter reduced. Fibers reduced the flow ability of the samples and viscosity increased. With increasing of the micro silica particles to cement ratio from 2/1 to 2/2, the slump flow diameter increased. By adding silica and alumina nanoparticles up to 3% and 2% respectively, the compressive strength increased and after decreased. Samples containing silica nanoparticles and fibers had the highest compressive strength.
Keywords: Portland cement, Composite, Nanoparticles, Compressive Strength.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19555830 Effect of Nanoparticle Diameter of Nano-Fluid on Average Nusselt Number in the Chamber
Authors: A. Ghafouri, N. Pourmahmoud, I. Mirzaee
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In this numerical study, effects of using Al2O3-water nanofluid on the rate of heat transfer have been investigated. Physical model is a square enclosure with insulated top and bottom horizontal walls, while the vertical walls are kept at different constant temperatures. Two appropriate models are used to evaluate the viscosity and thermal conductivity of nanofluid. The governing stream-vorticity equations are solved using a second order central finite difference scheme, coupled to the conservation of mass and energy. The study has been carried out for the nanoparticle diameter 30, 60 and 90 nm and the solid volume fraction 0 to 0.04. Results are presented by average Nusselt number and normalized Nusselt number in different range of φ and D for mixed convection dominated regime. It is found that different heat transfer rate is predicted when the effect of nanoparticle diameter is taken into account.Keywords: Nano-fluid, nanoparticle diameter, heat transfer enhancement, square enclosure, Nusselt number.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16945829 Forecasting Stock Price Manipulation in Capital Market
Authors: F. Rahnamay Roodposhti, M. Falah Shams, H. Kordlouie
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The aim of the article is extending and developing econometrics and network structure based methods which are able to distinguish price manipulation in Tehran stock exchange. The principal goal of the present study is to offer model for approximating price manipulation in Tehran stock exchange. In order to do so by applying separation method a sample consisting of 397 companies accepted at Tehran stock exchange were selected and information related to their price and volume of trades during years 2001 until 2009 were collected and then through performing runs test, skewness test and duration correlative test the selected companies were divided into 2 sets of manipulated and non manipulated companies. In the next stage by investigating cumulative return process and volume of trades in manipulated companies, the date of starting price manipulation was specified and in this way the logit model, artificial neural network, multiple discriminant analysis and by using information related to size of company, clarity of information, ratio of P/E and liquidity of stock one year prior price manipulation; a model for forecasting price manipulation of stocks of companies present in Tehran stock exchange were designed. At the end the power of forecasting models were studied by using data of test set. Whereas the power of forecasting logit model for test set was 92.1%, for artificial neural network was 94.1% and multi audit analysis model was 90.2%; therefore all of the 3 aforesaid models has high power to forecast price manipulation and there is no considerable difference among forecasting power of these 3 models.Keywords: Price Manipulation, Liquidity, Size of Company, Floating Stock, Information Clarity
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 28535828 On the Quantizer Design for Base Station Cooperation Systems with SC-FDE Techniques
Authors: K. Firsanov, S. Gritsutenko, R. Dinis
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By employing BS (Base Station) cooperation we can increase substantially the spectral efficiency and capacity of cellular systems. The signals received at each BS are sent to a central unit that performs the separation of the different MT (Mobile Terminal) using the same physical channel. However, we need accurate sampling and quantization of those signals so as to reduce the backhaul communication requirements. In this paper we consider the optimization of the quantizers for BS cooperation systems. Four different quantizer types are analyzed and optimized to allow better SQNR (Signal-to-Quantization Noise Ratio) and BER (Bit Error Rate) performance.
Keywords: Base Stations cooperation scheme, Bit Error Rate (BER), Quantizer, Signal to Quantization Noise Ratio (SQNR), SCFDE.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 17685827 Effects of Sodium Bicarbonate Content and Vulcanization Method on Properties of NBR/PVC Thermal Insulator Foam
Authors: P. Suriyachai, N. Thavarungkul, P. Sae-oui
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In this research sodium bicarbonate (NaHCO3) was introduced to generate carbon dioxide gas (CO2) to the existing nitrogen gas (N2) of elastomeric foam, to lower thermal conductivity (K). Various loadings of NaHCO3 (0 to 60 phr) were added into the azodicarbonamide (AZC)-containing compound and its properties were then determined. Two vulcanization methods, i.e., hot air and infrared (IR), were employed and compared in this study. Results revealed that compound viscosity tended to increase slightly with increasing NaHCO3 content but cure time was delayed. The effect of NaHCO3 content on thermal conductivity depended on the vulcanization method. For hot air method, the thermal conductivity was insignificantly changed with increasing NaHCO3 up to 40 phr whereas it tended to decrease gradually for IR method. At higher NaHCO3 content (60 phr), unexpected increase of thermal conductivity was observed. The water absorption was also determined and foam structures were then used to explain the results.
Keywords: sodium bicarbonate, thermal conductivity, hot airmethod, infrared method
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 37625826 Plaque Formation of Toxoplasma gondii in Vero Cells using Carboxymethylcellulose
Authors: L. Fonseca-Géigel, M. Alvarez, G. García, R. Cox, L. Morier, L. Fonte, M. G. Guzmán
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Toxoplasma gondii is an intracellular parasite capable of infecting all nucleated cells in a diverse array of species. Toxoplasma plaque assay have been described using Bacto Agar. Because of its experimental advantages carboxymethyl cellulose overlay, medium viscosity was choosing and the aim of this work was to develop alternative method for formation of T. gondii plaques. Tachyzoites were inoculated onto monolayers of Vero cells and cultured at 37° C under 5 % CO2. The cultures were followed up by microscopy inspection. Small plaques were visible by naphtol blue stain 4 days after infection. Larger plaques could be observed by day 10 of culture. The carboxymethyl cellulose is a cheap reagent and the methodology is easier, faster than assays under agar overlay. This is the first description of the carboxymethyl cellulose overlay use for obtaining the formation of T. gondii plaques and may be useful in consequent obtaining tachyzoites for detailed studies.Keywords: Carboxymethyl cellulose, Cell culture, Plaque assay, Toxoplasma gondii.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 27455825 A Completed Adaptive De-mixing Algorithm on Stiefel Manifold for ICA
Authors: Jianwei Wu
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Based on the one-bit-matching principle and by turning the de-mixing matrix into an orthogonal matrix via certain normalization, Ma et al proposed a one-bit-matching learning algorithm on the Stiefel manifold for independent component analysis [8]. But this algorithm is not adaptive. In this paper, an algorithm which can extract kurtosis and its sign of each independent source component directly from observation data is firstly introduced.With the algorithm , the one-bit-matching learning algorithm is revised, so that it can make the blind separation on the Stiefel manifold implemented completely in the adaptive mode in the framework of natural gradient.
Keywords: Independent component analysis, kurtosis, Stiefel manifold, super-gaussians or sub-gaussians.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15045824 Changes in Amino Acids Content in Muscle of European Eel (Anguilla anguilla) in Relation to Body Size
Authors: L. Gómez-Limia, I. Franco, T. Blanco, S. Martínez
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European eels (Anguilla anguilla) belong to Anguilliformes order and Anguillidae family. They are generally classified as warm-water fish. Eels have a great commercial value in Europe and Asian countries. Eels can reach high weights, although their commercial size is relatively low in some countries. The capture of larger eels would facilitate the recovery of the species, as well as having a greater number of either glass eels or elvers for aquaculture. In the last years, the demand and the price of eels have increased significantly. However, European eel is considered critically endangered by the International Union for the Conservation of Nature (IUCN) Red List. The biochemical composition of fishes is an important aspect of quality and affects the nutritional value and consumption quality of fish. In addition, knowing this composition can help predict an individual’s condition for their recovery. Fish is known to be important source of protein rich in essential amino acids. However, there is very little information about changes in amino acids composition of European eels with increase in size. The aim of this study was to evaluate the effect of two different weight categories on the amino acids content in muscle tissue of wild European eels. European eels were caught in River Ulla (Galicia, NW Spain), during winter. The eels were slaughtered in ice water immersion. Then, they were purchased and transferred to the laboratory. The eels were subdivided into two groups, according to the weight. The samples were kept frozen (-20 °C) until their analysis. Frozen eels were defrosted and the white muscle between the head and the anal hole. was extracted, in order to obtain amino acids composition. Thirty eels for each group were used. Liquid chromatography was used for separation and quantification of amino a cids. The results conclude that the eels are rich in glutamic acid, leucine, lysine, threonine, valine, isoleucine and phenylalanine. The analysis showed that there are significant differences (p < 0.05) among the eels with different sizes. Histidine, threonine, lysine, hydroxyproline, serine, glycine, arginine, alanine and proline were higher in small eels. European eels muscle presents between 45 and 46% of essential amino acids in the total amino acids. European eels have a well-balanced and high quality protein source in the respect of E/NE ratio. However, eels with higher weight showed a better ratio of essential and non-essential amino acid.
Keywords: European eels, amino acids, HPLC, body size.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 8365823 Graft Copolymerization of Methyl Methacrylate onto Cellulose in Homogeneous Medium – Effect of Solvent and Initiator
Authors: B. Tosh, C. R. Routray
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Homogeneous graft copolymerization of methyl methacrylate (MMA) onto cellulose was carried out in N, N – dimethyl acetamide/LiCl (DMAc/LiCl) and dimethyl sulfoxide/ paraformaldehyde (DMSO/PF) solvent system taking ceric ammonium nitrate (CAN), benzoyl peroxide (BPO) and tin (II)-2-ethyl hexanoate [Sn(Oct)2] as initiators. Different grafting parameters like graft yield (GY), grafting efficiency (GE) and total conversion of monomer to polymer (TC) were evaluated at different reaction conditions of temperature, time, and variation of the amount of monomer and initiator. The viscosity average molecular weight of grafted PMMA and number of grafts per cellulose chain were also calculated. The products were characterized by FT-IR and 1H-NMR analyses and possible reaction mechanisms were deduced. Thermal degradation of the grafted products was also studied by thermo-gravimetric analysis (TG) and differential thermo-gravimetry (DTG).
Keywords: Grafting, grafting efficiency, homogeneous medium, methyl methacrylate.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 31845822 A Wall Law for Two-Phase Turbulent Boundary Layers
Authors: Dhahri Maher, Aouinet Hana
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The presence of bubbles in the boundary layer introduces corrections into the log law, which must be taken into account. In this work, a logarithmic wall law was presented for bubbly two phase flows. The wall law presented in this work was based on the postulation of additional turbulent viscosity associated with bubble wakes in the boundary layer. The presented wall law contained empirical constant accounting both for shear induced turbulence interaction and for non-linearity of bubble. This constant was deduced from experimental data. The wall friction prediction achieved with the wall law was compared to the experimental data, in the case of a turbulent boundary layer developing on a vertical flat plate in the presence of millimetric bubbles. A very good agreement between experimental and numerical wall friction prediction was verified. The agreement was especially noticeable for the low void fraction when bubble induced turbulence plays a significant role.Keywords: Bubbly flows, log law, boundary layer.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 11265821 Lubrication Performance of Multi-Level Gear Oil in a Gasoline Engine
Authors: Feng-Tsai Weng, Dong- Syuan Cai, Tsochu-Lin
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A vehicle gasoline engine converts gasoline into power so that the car can move, and lubricants are important for engines and also gear boxes. Manufacturers have produced numbers of engine oils, and gear oils for engines and gear boxes to SAE International Standards. Some products not only can improve the lubrication of both the engine and gear box but also can raise power of vehicle this can be easily seen in the advertisement declared by the manufacturers. To observe the lubrication performance, a multi-leveled (heavy duty) gear oil was added to a gasoline engine as the oil in the vehicle. The oil was checked at about every 10,000 kilometers. The engine was detailed disassembled, cleaned, and parts were measured. The wear of components of the engine parts were checked and recorded finally. Based on the experiment results, some gear oil seems possible to be used as engine oil in particular vehicles. Vehicle owners should change oil periodically in about every 6,000 miles (or 10,000 kilometers). Used car owners may change engine oil in even longer distance.
Keywords: Multi-level gear oil, engine oil, viscosity, abrasion.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10685820 Enhancing the Peer-To-Peer Architecture with a Roaming Service and OWL
Authors: Younes Djaghloul, Zizette Boufaida
Abstract:
This paper addresses the problem of building a unified structure to describe a peer-to-peer system. Our approach uses the well-known notations in the P2P area, and provides a global architecture that puts a separation between the platform specific characteristics and the logical ones. In order to enable the navigation of the peer across platforms, a roaming layer is added. The latter provides a capability to define a unique identification of peer and assures the mapping between this identification and those used in each platform. The mapping task is assured by special wrapper. In addition, ontology is proposed to give a clear presentation of the structure of the P2P system without interesting in the content and the resource managed by the peer. The ontology is created according to the web semantic paradigm and using OWL language; so, the structure of the system is considered as a web resource.Keywords: Peer to peer, ontology, owl.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1378