Search results for: Sperm Concentration

Authors: Sri Wahjuningsih, Hermanto, Nuryadi, Agus Budiarto, Panji Bhintoro

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The objective of the present study was to determine the effect of different concentration of spermatozoa and length of storage in 5 0C on sperm motility. Semen was collected using artificial vagina from goat aged 2 to 2.5 years. Fresh goat semen with sperm motility ≥ 70% was used as material. Semen was divided into 4 treatments of concentration (40 x 10 6 / ml, 50 x 106/ml, 60x106/ml, 70x106/ml) with length of storage 0,12,24,36 h. in 5 0C. There were interactions (P <0.05) between concentration and length of storage on individual motility of spermatozoa. Concentration of spermatozoa and length of storage affect the motility of individual (P <0.05). It was concluded that Sperm motility will decrease with increasing concentration and length of storage in 5 0C. Concentration of sperm 40x106/ ml and length of storage 0 h in 50C showed the highest motility of spermatozoa

Keywords: Goat, Length of storage, Motility, Sperm Concentration

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Authors: Radosław K. Kowalski, Beata Sarosiek, Wiesław Demianowicz, Jędrek Judek, Krzysztof Goryczko, Stefan Dobosz, Henryk Kuźmiński, Krystyna Demska-Zakęś, Igor Babiak, Jan Glogowski

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Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Sertoli cells.

Keywords: fish, genotype, rainbow trout, sperm.

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Authors: Immanuel I. Bitto, Thomas Agam

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28 healthy adult Maradi bucks were used to evaluate sperm production and sperm storage capacity in the breed. Daily sperm production (DSP) averaged 0.55±0.05x109, while the daily sperm production/g (DSP/g) was 1.37±0.12 x107. Gonadal sperm reserve was 1.99±0.18 x109, while the caput, upper corpus and lower corpus averaged 0.58±0.04 x109, 0.36±0.02 x109 and 0.33±0.08 x109 respectively. The proximal cauda, mid cauda, distal cauda and ductus deferens had values of 0.68±0.10 x109, 1.23±0.16 x109,1.87±0. x109and 0.17±0.05 x109 respectively. The relative contributions of the respective epididymal sections and ductus deferens to the total extragonadal sperm reserves were, 11.11%, 6.89%, 6.32%, 13.03%, 23.56%, 35.82% and 3.26% respectively. Gonadal sperm reserves were significantly higher (p<0.05) than caput reserves, upper corpus reserves, lower corpus reserves, proximal cauda reserves and ductus deferens reserves. Gonadal reserves were however similar (p>0.05) to mid cauda and distal cauda epididymal reserves.

Keywords: Goats, Reserves, Sperm, Tropics

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Authors: I. Nur Hilwani, R. Nasibah, S. Nurdiana, M. J. Norashirene

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Impaired fertility may be the result of indirect consumption of anti-fertility agents through food. Monosodium glutamate (MSG) has been widely used as food additive, flavour enhancer and included in vaccines. This study focuses in determining the gonadotoxic and cytotoxic effect of MSG on selected sperm parameters such as sperm viability, sperm membrane integrity and testes cytoarchitecture of male mice via histological examination to determine its effect on spermatogenesis. Twenty-four Mus musculus were randomly divided into 4 groups and given intraperitoneal injections (IP) daily for 14 days of different MSG concentrations at 250, 500 and 1000mg/kg MSG to body weight to induce obesity. Saline was given to control group. Mice were sacrificed and analysis revealed abnormalities in values for sperm parameters and damages to testes cytoarchitecture of male mice. The results recorded decreased viability (p<0.05) and integrity of sperm membrane (p>0.05) with degenerative structures in seminiferous tubule of testes. The results indicated various implications of MSG on male mice reproductive system which has consequences in fertility potential.

Keywords: Sperm parameter, sperm viability, sperm membrane integrity and testes histology.

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Authors: Nikolai V. Barulin, Vitaly Yu. Plavskii

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This work contains information about the influence low-level optical irradiation on sperm motility of sturgeon fish. On the basis of given and earlier received data the following conclusion has been made. Among the photophysical processes of a resonant and not resonant nature (oriented action of light; action of gradient forces; dipole-dipole interaction; termooptical processes), which are capable to cause the photobiological effects depended on such laserspecific characteristics as polarization and coherency, determining influence belongs to oriented action of light and dipole-dipole interactions among the processes studied in the present work.

Keywords: sturgeon, aquaculture, fish sperm, laser, optical irradiation, sperm motility

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Authors: Dike H. Ogbuagu, Etsede J. Oritsematosan

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This work investigated possible inductions of CaC₂, often misused by fruit vendors to stimulate artificial ripening, on mammalian sperm morphology and viability. Thirty isogenic strains of male albino mice, Mus musculus (age≈ 8weeks; weight= 32.52.0g) were acclimatized (ambient temperature 28.0±1.0°C) for 2 weeks and fed standard growers mash and water ad libutum. They were later exposed to graded toxicant concentrations (w/w) of 2.5000, 1.2500, 0.6250, and 0.3125% in 4 cages. A control cage was also established. After 5 weeks, 3 animals from each cage were sacrificed by cervical dislocation and the cauda epididymis excised. Sperm morphology and viability were determined by microscopic procedures. The ANOVA, means plots, Student’s t-test and variation plots were used to analyze data. The common abnormalities observed included Double Head, Pin Head, Knobbed Head, No Tail and With Hook. The higher toxicant concentrations induced significantly lower body weights [F_(829.899) ˃ F_crit(4.19)] and more abnormalities [F_(26.52) ˃ F_crit(4.00)] at P˂0.05. Sperm cells in the control setup were significantly more viable than those in the 0.625% (t=0.005) and 2.500% toxicant doses (t=0.018) at the 95% confidence limit. CaC₂ appeared to induced morphological abnormalities and reduced viability in sperm cells of M. musculus.

Keywords: Artificial ripening, Calcium carbide, fruit vendors, sperm morphology, sperm viability.

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Authors: Vahid Reza Nafisi, Mohammad Hasan Moradi, Mohammad Hosain Nasr-Esfahani

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The conventional assessment of human semen is a highly subjective assessment, with considerable intra- and interlaboratory variability. Computer-Assisted Sperm Analysis (CASA) systems provide a rapid and automated assessment of the sperm characteristics, together with improved standardization and quality control. However, the outcome of CASA systems is sensitive to the method of experimentation. While conventional CASA systems use digital microscopes with phase-contrast accessories, producing higher contrast images, we have used raw semen samples (no staining materials) and a regular light microscope, with a digital camera directly attached to its eyepiece, to insure cost benefits and simple assembling of the system. However, since the accurate finding of sperms in the semen image is the first step in the examination and analysis of the semen, any error in this step can affect the outcome of the analysis. This article introduces and explains an algorithm for finding sperms in low contrast images: First, an image enhancement algorithm is applied to remove extra particles from the image. Then, the foreground particles (including sperms and round cells) are segmented form the background. Finally, based on certain features and criteria, sperms are separated from other cells.

Keywords: Computer-Assisted Sperm Analysis (CASA), Sperm identification, Tail detection, Elliptic shape model.

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Authors: M. M. Seif, F. A. Khalil, A. A. K. Abou Arab, A. S. Abdel- Aziz, M. A. Abou Donia, Sh. R. Mohamed

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Malathion (ML) is a well known pesticide commonly used in many agricultural and non-agricultural processes. Its toxicity has been attributed primarily to the accumulation of acetylcholine (Ach) at nerve junctions, due to the inhibition of acetylcholinesterase (AChE). The aim of the current research was to study the protective effect of the melissa plant extract against reproductive impairment induced by malathion in 32 male albino rats, and the biological experiment was divided into four groups (8 in each) that given malathion (27 mg/kg; 1/50 of the LD50 for an oral dose) and/or Melissa officinalis (MO) extract (200mg/kg/day) by gavages technique. The sperm counts, sperm motility, sperm morphology, FSH, LH, and testosterone levels had been determined in testes homogenate at the end of the experiment. It is worthy to report that, rats treated with melissa extract did not show a significant difference when compared with the control group, while rats given malathion alone had significantly lower sperm count, sperm motility, and significantly higher abnormal sperm numbers, than the untreated control rats as well as having significantly lower serum FSH, LH, and testosterone levels compared with the control group. Administrations of melissa extract restore all mentioned histological parameters towards the control group and the melissa extract had a strong positive protective effect against malathion toxicity. Results the of biological parameters were confirmed by the histological examination of rat testes and indicated that, both control and melissa groups showing normal seminiferous tubules, while malathion group testicular tissues had necrosis, edema in the seminiferous tubules and degeneration of spermatogonial cells lining the seminiferous tubules with incomplete spermatogenesis. The use of melissa against malathion improved the histological picture and showing normal seminiferous tubules with complete spermatogenesis and almost there was no histopathological changes could be noted.

Keywords: Malathion, Melissa officinalis L., Reproductive toxicity, Rats.

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Authors: Fereidoon Nowshiravan Rahatabad, Mohammad Hassan Moradi, Vahid Reza Nafisi

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Nothing that an effective cure for infertility happens when we can find a unique solution, a great deal of study has been done in this field and this is a hot research subject for to days study. So we could analyze the men-s seaman and find out about fertility and infertility and from this find a true cure for this, since this will be a non invasive and low risk procedure, it will be greatly welcomed. In this research, the procedure has been based on few Algorithms enhancement and segmentation of images which has been done on the images taken from microscope in different fertility institution and have obtained a suitable result from the computer images which in turn help us to distinguish these sperms from fluids and its surroundings.

Keywords: Computer-Assisted Sperm Analysis (CASA), Spermidentification, Segmentation.

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Authors: Pascal Constantin, Gîlcă Ioan, Radu Rusu Răzvan, Nacu Gherasim

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In the Northern hemisphere, sheep reproduction is seasonal (September-November). Among several natural factors influencing the reproduction status of rams, we studied the daylight length and temperature. Rams from different breeds were studied: Merinos de Palas (half-precocious), Karakul de Botosani (halfbelated) and Turcana (belated breed, low reproductive plasticity). In Merinos de Palas, ejaculate volume during sexual repose is 51.3% from normal quantity. When autumn climate was experimentally induced, ejaculate volume reached 98.45% (Merinos), 94.97% (Karakul) and 97.59% (Turcana). Semen density increased from 1.031-1.033 till 1.035 after exposition to artificial light and temperature conditions. Spermatozoids mobility and sperm pH improved, passing over 82% and 6.75, values identical to those in the natural reproduction season. Behaviour analysis after photoperiodicity indicated that over 83.3% Merinos and Karakul males and all Turcana rams exteriorised normal and intense sexual reflexes. Certain effort and reduced expenses brought rams in good condition, producing higher quantity and quality sperm.

Keywords: natural factors, photoperiodicity, sexual behaviour, Romanian sheep.

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Authors: Eva Tvrdá, Boris Botman, Marek Halenár, Tomáš Slanina, Norbert Lukáč

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In vitro storage and processing of animal semen represents a risk factor to spermatozoa vitality, potentially leading to reduced fertility. A variety of substances isolated from natural sources may exhibit protective or antioxidant properties on the spermatozoon, thus extending the lifespan of stored ejaculates. This study compared the ability of different concentrations of the Salvia officinalis extract on the motility, mitochondrial activity, viability and reactive oxygen species (ROS) production by bovine spermatozoa during different time periods (0, 2, 6 and 24 h) of in vitro culture. Spermatozoa motility was assessed using the Computer-assisted sperm analysis (CASA) system. Cell viability was examined using the metabolic activity MTT assay, the eosin-nigrosin staining technique was used to evaluate the sperm viability and ROS generation was quantified using luminometry. The CASA analysis revealed that the motility in the experimental groups supplemented with 0.5-2 µg/mL Salvia extract was significantly lower in comparison with the control (P<0.05; Time 24 h). At the same time, a long-term exposure of spermatozoa to concentrations ranging between 0.05 µg/mL and 2 µg/mL had a negative impact on the mitochondrial metabolism (P<0.05; Time 24 h). The viability staining revealed that 0.001-1 µg/mL Salvia extract had no effects on bovine male gametes, however 2 µg/mL Salvia had a persisting negative effect on spermatozoa (P<0.05). Furthermore 0.05-2 µg/mL Salvia exhibited an immediate ROS-promoting effect on the sperm culture (P>0.05; Time 0 h and 2 h), which remained significant throughout the entire in vitro culture (P<0.05; Time 24 h). Our results point out to the necessity to examine specific effects the biomolecules present in Salvia officinalis may have individually or collectively on the in vitro sperm vitality and oxidative profile.

Keywords: Bulls, CASA, MTT test, reactive oxygen species, sage, Salvia officinalis, spermatozoa.

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Authors: S. Shalaweh, P. Bouic, F. Weitz, R. Henkel

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More than 3000 plants of notable phyto-therapeutic value grow in South Africa; these include Cissampelos capensis, commonly known in Afrikaans as dawidjie or dawidjiewortel. C. capensis is the most significant and popular medicinal plant used by the Khoisan as well as other rural groups in the Western region of South Africa. Its rhizomes are traditionally used to treat male fertility problems. Yet, no studies have investigated the effects of this plant or its extracts on human spermatozoa. Therefore, this study aimed at investigating the effects of C. capensis rhizome extract (CRE) fractions on ejaculated human spermatozoa in vitro. Spermatozoa from a total of 77 semen samples were washed with human tubular fluid medium supplemented with bovine serum albumin (HTF-BSA) and incubated for 2 hours with 20 μg/ml progesterone (P4) followed by incubation with different concentrations (0, 0.05, 0.5, 5, 50, 200 μg/ml) of fractionated CRE (F1=0% MeOH, F2=30% MeOH, F3=60% MeOH and F4=100% MeOH) for 1.5 hours at 37°C. A sample without addition of CRE fractions served as control. Samples were analyzed for sperm motility, reactive oxygen species (ROS), DNA-fragmentation, acrosome reaction and capacitation. Results showed that F1 resulted in significantly higher values for ROS, capacitation and hyper-activation compared to F2, F3, and F4 with P4-stimulated samples generally having higher values. No significant effect was found for the other parameters. In conclusion, alkaloids present in F1 of CRE appear to have triggered sperm intrinsic ROS production leading to sperm capacitation and acrosome reaction induced by P4.

Keywords: Capacitation, acrosome reaction, Cissampelos capensis, DNA fragmentation, ROS.

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Authors: Agata Kowalska, Radosław K. Kowalski, Zdzisław Zakęś

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Our results showed that treatment with both cyclooxygenase (COX1 or COX2) inhibitors impair reproduction parameters of the medaka. Resveratrol (COX1 inhibitor) caused an decrease in the number of spawning females at the first week of feeding fish with experimental diets. In the group treated with NS- 398 (COX2 inhibitor) we found the lowest sperm velocity parameters and decreased linearity of movement. The ovaries of the medaka fed feed supplemented with Resveratrol or NS-398 were confirmed to have a lower share of matured oocytes however during the experiment (four weeks) the number of eggs spawned by females was similar. Both inhibitors in fish diet (20 mg/kg body weight/day) caused a decrease in the embryo survival. Our results revealed that for the medaka female reproduction, activity of both COX enzymes might be necessary whereas males reproduction competence, as expressed by sperm motility parameters, might be related to COX2 activity.

Keywords: COX innibitors, medaka, reproduction parameters

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Authors: T. Pavic Kramaric, M. Kitic

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The purpose of this article is to analyze the market structure as well as the degree of concentration in insurance markets in new EU member states. The analysis was conducted using several most commonly used concentration indicators such as concentration ratio, Herfindahl-Hirschman index and entropy index. These indicators were calculated for the 2000-2010 period on the basis of total gross written premium as the most relevant indicator of market power in insurance markets. The results of the analysis showed that in all observed countries the level of concentration decreased, though with significantly different intensity. Yet, in some countries, the level of concentration remains very high.

Keywords: insurance market, concentration, new EU member states

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Authors: Olivier Adam, Maciej Lopatka, Christophe Laplanche, Jean-François Motsch

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This article presents the results using a parametric approach and a Wavelet Transform in analysing signals emitting from the sperm whale. The extraction of intrinsic characteristics of these unique signals emitted by marine mammals is still at present a difficult exercise for various reasons: firstly, it concerns non-stationary signals, and secondly, these signals are obstructed by interfering background noise. In this article, we compare the advantages and disadvantages of both methods: Auto Regressive models and Wavelet Transform. These approaches serve as an alternative to the commonly used estimators which are based on the Fourier Transform for which the hypotheses necessary for its application are in certain cases, not sufficiently proven. These modern approaches provide effective results particularly for the periodic tracking of the signal's characteristics and notably when the signal-to-noise ratio negatively effects signal tracking. Our objectives are twofold. Our first goal is to identify the animal through its acoustic signature. This includes recognition of the marine mammal species and ultimately of the individual animal (within the species). The second is much more ambitious and directly involves the intervention of cetologists to study the sounds emitted by marine mammals in an effort to characterize their behaviour. We are working on an approach based on the recordings of marine mammal signals and the findings from this data result from the Wavelet Transform. This article will explore the reasons for using this approach. In addition, thanks to the use of new processors, these algorithms once heavy in calculation time can be integrated in a real-time system.

Keywords: Autoregressive model, Daubechies Wavelet, Fourier Transform, marine mammals, signal processing, spectrogram, sperm whale, Wavelet Transform.

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Authors: Hamid. Gholami, Mohammad. Chamani, Armin. Towhidi, Mohammad. H. Fazeli

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The aim of current study was to investigate the changes in the quality parameters of Holstein bull semen during the heat stress and the effect of feeding a source of omega-3 fatty acids in this period. Samples were obtained from 19 Holstein bulls during the expected time of heat stress in Iran (June to September 2009). Control group (n=10) were fed a standard concentrate feed while treatment group (n=9) had this feed top dressed with 100 g of an omega-3 enriched nutriceutical. Semen quality was assessed on ejaculates collected after 1, 5, 9 and 12 weeks of supplementation. Computer-assisted assessment of sperm motility, viability (eosinnigrosin) and hypo-osmotic swelling test (HOST) were conducted. Heat stress affected sperm quality parameters by week 5 and 9 (p<0.05). Supplementation has significantly increased total motility, progressive motility, HOST and average path velocity in the fresh semen of bulls (P<0.05).

Keywords: Bull, heat stress, omega-3 fatty acids, spermatozoa.

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Authors: Ivan Pavic, Fran Galetic, Tomislava Pavic Kramaric

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The purpose of this article is to analyze the degree of concentration in the banking market in EU member states as well as to determine the impact of the length of EU membership on the degree of concentration. In that sense several analysis were conducted, specifically, panel analysis, calculation of correlation coefficient and regression analysis of the impact of the length of EU membership on the degree of concentration. Panel analysis was conducted to determine whether there is a similar trend of concentration in three groups of countries - countries with a low, moderate and high level of concentration. The conducted panel analysis showed that in EU countries with a moderate level of concentration, the level of concentration decreases. The calculation of correlation showed that, to some extent, with other influential factors, the length of EU membership negatively affects the market concentration of the banking market. Using the regression analysis for investigation of the influence of the length of EU membership on the level of concentration in the banking sector in a particular country, the results reveal that there is a negative effect of the length in EU membership on market concentration, although it is not significantly influential variable.

Keywords: Banking sector, concentration, EU

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Authors: S. Suyadi

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Sexual behavior and semen charactertistics were evaluated in young male Boer goats in tropical condition during time period of September to November 2009. The animal was let to have adaptation for five months after importation from Australian climate. A total of 20 bucks were observed for sexual behavior and ability of semen production. Out of this number, 4 faild to libido and 3 produced poor semen. The remaing 13 animals were divided into three groups according to the ages (11-13, 15-16 and 18-25 months). Sexual behavior consisting response time to female teaser, ejaculation time, fixing strenght to female and erection status were normaly observer in 13 bucks, and there was no significant difference between age groups. Semen characteristics from 13 bucks were in normal quality in the volume, sperm mass motility, individual motility, percentage of live- and abnormal sperm. We concluded that is possible to collect semen of Boer goats during the period of September to November under tropical condition. Collection during other time period should be analyzed.

Keywords: sexsual behavior, semen characteristics, Boer goats, tropical condition.

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Authors: Ismail I. Abo-Ghanema, El-Nasharty M.A., El-Far A. H., Hanan A.Ghonium

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In this study, we investigated the effects of ginger and L-carnitine on the reproductive performance of male rats with respect to semen parameters, male sex hormones and the testicular antioxidant system. A total of sixty mature male albino rats were divided into four groups of fifteen rats. The control group received saline, whereas the other three groups received ginger (100 mg kg-1 d- 1.), L-carnitine (150 mg kg-1 d-1.) or a combination of both ginger (100 mg kg-1 d-1.) and L-carnitine (150 mg kg-1 d-1.) via a stomach tube daily for one month. At the end of the treatment period, the rats were sacrificed, and their sperm characteristics (count, motility and viability), antioxidant enzyme factors levels (reduced glutathione, catalase, superoxide dismutase and total antioxidant capacity) and sex hormone levels (testosterone, Follicle stimulating hormone(FSH) and luteinizing hormone (LH) were analysed. Our results showed that the three experimental treatments improved sperm parameters, antioxidant enzyme activity and testosterone hormone levels; the most pronounced positive effects were observed in the group that received a combination of both ginger and L-carnitine. Therefore, the administration of a combination of ginger and L-carnitine may be beneficial for improving male sexual performance.

Keywords: Ginger, L-Carnitine, Spermatogenesis, Rats.

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Authors: Matura Unhasirikul, Nuanphan Naranong, Woatthichai Narkrugsa

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Agricultural waste is mainly composed of cellulose and hemicelluloses which can be converted to sugars. The inexpensive reducing sugar from durian peel was obtained by hydrolysis with HCl concentration at 0.5-2.0% (v/v). The hydrolysis range of time was for 15-60 min when the mixture was autoclaved at 121 °C. The result showed that acid hydrolysis efficiency (AHE) highest to 80.99% at condition is 2.0%concentration for 15 min. Reducing sugar highest to 56.07 g/litre at condition is 2.0% concentration for 45min. Total sugar highest to 59.83 g/litre at condition is 2.0%concentration for 45min, which was not significant (p < 0.05) with condition 2.0% concentration for 30 min and 1.5 % concentration for 45 and 60 min. The increase in concentration increased AHE, reducing sugar and total sugar. The hydrolysis time had no effect on AHE, reducing sugar and total sugar. The maximum reducing sugars of each concentration were at hydrolysis time 45 min .The hydrolysated were analysis by HPLC, the results revealed that the principle of sugar were glucose, fructose and xylose.

Keywords: acid hydrolysis efficiency (AHE), reducing sugar, total sugar

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Authors: Ek Ngoy, A F Mulaba-Bafubiandi

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Cobalt was acid nitric leached from a mixed cobaltcopper oxide with variable acid concentration. Resulting experimental data were used to analyze effects of increase in acid concentration, based on a shrinking core model of the process. The mathematical simulation demonstrated that the time rate of the dissolution mechanism is an increasing function of acid concentration. It was also shown that the magnitude of the acid concentration effect is time dependent and the increase in acid concentration is more effective at earlier stage of the dissolution than at later stage. The remaining process parameters are comprehensively affected by acid concentration and their interaction is synergetic.

Keywords: Acid effect, Cobalt, Cobalt-copper oxide, Leaching, Simulation

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Authors: W. L. Liong, Srimala Sreekantan, Sabar D. Hutagalung

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The effect of concentration of reduction agent of sodium borohydrate (NaBH4) on the properties of silicon nanoparticles synthesized via microemulsion route is reported. In this work, the concentration of the silicon tetrachloride (SiCl4) that served as silicon source with sodium hydroxide (NaOH) and polyethylene glycol (PEG) as stabilizer and surfactant, respectively, are keep fixed. Four samples with varied concentration of NaBH4 from 0.05 M to 0.20 M were synthesized. It was found that the lowest concentration of NaBH4 gave better formation of silicon nanoparticles.

Keywords: Microelmusion, nanoparticles, reduction, silicon

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Authors: Marek Halenár, Eva Tvrdá, Tomáš Slanina, Ľubomír Ondruška, Eduard Kolesár, Peter Massányi, Adriana Kolesárová

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The present in vitro study was designed to reveal whether amygdalin (AMG) is able to cause changes to the motility, viability and mitochondrial activity of rabbit spermatozoa. New Zealand White rabbits (n = 10) aged four months were used in the study. Semen samples were collected from each animal and used for the in vitro incubation. The samples were divided into five equal parts and diluted with saline supplemented with 0, 0.5, 1, 2.5 and 5 mg/mL AMG. At times 0h, 3h and 5h spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system, cell viability was examined with the metabolic activity (MTT) assay, and the eosin-nigrosin staining technique was used to evaluate the viability of rabbit spermatozoa. All AMG concentrations exhibited stimulating effects on the spermatozoa activity, as shown by a significant preservation of the motility (P<0.05 with respect to 0.5 mg/mL and 1 mg/mL AMG; Time 5 h) and mitochondrial activity (P< 0.05 in case of 0.5 mg/mL AMG; P< 0.01 in case of 1 mg/mL AMG; P < 0.001 with respect to 2.5 mg/mL and 5 mg/mL AMG; Time 5 h). None of the AMG doses supplemented had any significant impact of the spermatozoa viability. In conclusion, the data revealed that short-term co-incubation of spermatozoa with AMG may result in a higher preservation of the sperm structural integrity and functional activity.

Keywords: Amygdalin, CASA, mitochondrial activity, motility, rabbits, spermatozoa, viability.

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Authors: K. Manikandan, T. Viruthagiri

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Studies on Simultaneous Saccharification and Fermentation (SSF) of corn flour, a major agricultural product as the substrate using starch digesting glucoamylase enzyme derived from Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces cerevisiae in a batch fermentation. Experiments based on Central Composite Design (CCD) were conducted to study the effect of substrate concentration, pH, temperature, enzyme concentration on Ethanol Concentration and the above parameters were optimized using Response Surface Methodology (RSM). The optimum values of substrate concentration, pH, temperature and enzyme concentration were found to be 160 g/l, 5.5, 30°C and 50 IU respectively. The effect of inoculums age on ethanol concentration was also investigated. The corn flour solution equivalent to 16% initial starch concentration gave the highest ethanol concentration of 63.04 g/l after 48 h of fermentation at optimum conditions of pH and temperature. Monod model and Logistic model were used for growth kinetics and Leudeking – Piret model was used for product formation kinetics.

Keywords: Simultaneous Saccharification and Fermentation(SSF), Corn Starch, Ethanol, Logisitic Model.

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Authors: Tae Jin Choi, Jong Ok Kim, Sang Min Jin, Gilwon Yoon

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Analysis of EEG brainwave provides information on mental or emotional states. One of the particular states that can have various applications in human machine interface (HMI) is concentration. 8-channel EEG signals were measured and analyzed. The concentration index was compared during resting and concentrating periods. Among eight channels, locations the frontal lobe (F_p1 and F_p2) showed a clear increase of the concentration index during concentration regardless of subjects. The rest six channels produced conflicting observations depending on subjects. At this time, it is not clear whether individual difference or how to concentrate made these results for the rest six channels. Nevertheless, it is expected that F_p1 and F_p2 are promising locations for extracting control signal for HMI applications.

Keywords: Concentration, EEG, human machine interface.

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Authors: Parveen K. Saini, Tarun Agarwal

Abstract:

An investigation into the effect of countersunk depth, plate thickness, countersunk angle and plate width on the stress concentration around countersunk hole is carried out with the help of finite element analysis. The variation of stress concentration with respect to these parameters is studied for three types of loading viz. uniformly distributed load, uniformly varying load and functionally distributed load. The results of the finite element analysis are interpreted and some conclusions are drawn. The distribution of stress concentration around countersunk hole in isotropic plates simply supported at all the edges is found similar and is independent of loading. The maximum stress concentration also occurs at a particular point irrespective of the loading conditions.

Keywords: Stress Concentration Factor, Countersunk hole, Finite element, ANSYS.

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Authors: P. Massanyi, L. Kichi, T. Slanina, E. Kolesar, J. Danko, N. Lukac, E. Tvrda, R. Stawarz, A. Kolesarova

Abstract:

Target of this study was the analysis of the impact of crude glycerol on canine spermatozoa motility, morphology, viability, and membrane integrity. Experiments were realized in vitro. In the study, semen from 5 large dog breeds was used. They were typical representatives of large breeds, coming from healthy rearing, regularly vaccinated and integrated to the further breeding. Semen collections were realized at the owners of animals and in the veterinary clinic. Subsequently the experiments were realized at the Department of Animal Physiology of the SUA in Nitra. The spermatozoa motility was evaluated using CASA analyzer (SpermVisionTM, Minitub, Germany) at the temperature 5 and 37°C for 5 hours. In the study, 13 motility parameters were evaluated. Generally, crude glycerol has generally negative effect on spermatozoa motility. Morphological analysis was realized using Hancock staining and the preparations were evaluated at magnification 1000x using classification tables of morphologically changed spermatozoa. Data clearly detected the highest number of morphologically changed spermatozoa in the experimental groups (know twisted tails, tail torso and tail coiling). For acrosome alterations swelled acrosomes, removed acrosomes and acrosomes with undulated membrane were detected. In this study also the effect of crude glycerol on spermatozoa membrane integrity were analyzed. The highest crude glycerol concentration significantly affects spermatozoa integrity. Results of this study show that crude glycerol has effect of spermatozoa motility, viability, and membrane integrity. Detected changes are related to crude glycerol concentration, temperature, as well as time of incubation.

Keywords: Dog, semen, spermatozoa, acrosome, glycerol, CASA, viability.

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Authors: R. Nagendra Babu, K. V. Ramana, K. Mallikarjuna Rao

Abstract:

Stress Concentration Factors are significant in machine design as it gives rise to localized stress when any change in the design of surface or abrupt change in the cross section occurs. Almost all machine components and structural members contain some form of geometrical or microstructural discontinuities. These discontinuities are very dangerous and lead to failure. So, it is very much essential to analyze the stress concentration factors for critical applications like Turbine Rotors. In this paper Finite Element Analysis (FEA) with extremely fine mesh in the vicinity of the blades of Steam Turbine Rotor is applied to determine stress concentration factors. A model of Steam Turbine Rotor is shown in Fig. 1.

Keywords: Stress Concentration Factors, Finite Element Analysis, and ANSYS.

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Authors: Abbas R. Ali, Safa H. Abdulrahman

Abstract:

In this study, forty samples had been collected from water of Lesser Zab River and drinking water to determine fluoride concentration and show the impact of fluoride on general health of society of Kirkuk city. Estimation of fluoride concentration and determination of its proportion in water samples were performed attentively using a fluoride ion selective electrode. The fluoride concentrations in the Lesser Zab River samples were between 0.0265 ppm and 0.0863 ppm with an average of 0.0451 ppm, whereas the average fluoride concentration in drinking water samples was 0.102 ppm and ranged from 0.010 to 0.289 ppm. A comparison between results obtained with World Health Organization (WHO) show a low concentration of fluoride in the samples of the study. Thus, for health concerns we should increase the concentration of this ion in water of Kirkuk city at least to about (1.0 ppm) and this will take place after fluorination process.

Keywords: Fluoride concentration, Lesser Zab River, drinking water, health society, Kirkuk city.

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Authors: Gautham Das, Edward Morrone, Erik Treble, Clinton Binder

Abstract:

The purpose of this study was to evaluate the efficacy of a low-cost filter regarding per- and polyfluoroalkyl substances (PFAS). PFAS is a commonly used man-made chemical that can be found in a variety of household and industrial products with deleterious effects on humans. The filter consists of a combination of low-cost materials which could be locally procured. Water testing results for 4 different PFAS contaminants indicated that for Perfluorooctane sulfonic acid (PFOS), the Agency for Toxic Substances and Disease Registry (ATSDR) regulation is 7 ppt, the initial concentration was 15 ppt, and the final concentration was 3.9 ppt. For Perfluorononanoic acid (PFNA), the ATSDR regulation is 10.5 ppt, the initial concentration was 15 ppt, and the final concentration was 3.9 ppt. For Perfluorooctanoic acid (PFOA), the ATSDR regulation is 11 ppt, the initial concentration was 15 ppt, and the final concentration was 3.9 ppt. For Perfluorohexane sulfonic acid (PFHxS), the ATSDR regulation is 70 ppt, the initial concentration was 15 ppt, and the final concentration was 3.9 ppt. The results indicated a 74% reduction in PFAS concentration in filtered samples. Statistical data through regression analysis showed 0.9 validity of the sample data. Initial tests show the efficiency of the proposed filter described could be far greater if tested at a greater scale. It is highly recommended further testing to be conducted to validate the data for an innovative solution to a ubiquitous problem.

Keywords: PFAS, PFOS, PFOA, PFHxS, low-cost filter.

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