%0 Journal Article
	%A P. Massanyi and  L. Kichi and  T. Slanina and  E. Kolesar and  J. Danko and  N. Lukac and  E. Tvrda and  R. Stawarz and  A. Kolesarova
	%D 2015
	%J International Journal of Animal and Veterinary Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 107, 2015
	%T Crude Glycerol Affects Canine Sperm Motility: Computer Assisted Semen Analysis in vitro
	%U https://publications.waset.org/pdf/10002589
	%V 107
	%X Target of this study was the analysis of the impact of
crude glycerol on canine spermatozoa motility, morphology,
viability, and membrane integrity. Experiments were realized in vitro.
In the study, semen from 5 large dog breeds was used. They were
typical representatives of large breeds, coming from healthy rearing,
regularly vaccinated and integrated to the further breeding. Semen
collections were realized at the owners of animals and in the
veterinary clinic. Subsequently the experiments were realized at the
Department of Animal Physiology of the SUA in Nitra. The
spermatozoa motility was evaluated using CASA analyzer
(SpermVisionTM, Minitub, Germany) at the temperature 5 and 37°C
for 5 hours. In the study, 13 motility parameters were evaluated.
Generally, crude glycerol has generally negative effect on
spermatozoa motility. Morphological analysis was realized using
Hancock staining and the preparations were evaluated at
magnification 1000x using classification tables of morphologically
changed spermatozoa. Data clearly detected the highest number of
morphologically changed spermatozoa in the experimental groups
(know twisted tails, tail torso and tail coiling). For acrosome
alterations swelled acrosomes, removed acrosomes and acrosomes
with undulated membrane were detected. In this study also the effect
of crude glycerol on spermatozoa membrane integrity were analyzed.
The highest crude glycerol concentration significantly affects
spermatozoa integrity. Results of this study show that crude glycerol
has effect of spermatozoa motility, viability, and membrane integrity.
Detected changes are related to crude glycerol concentration,
temperature, as well as time of incubation.
	%P 1151 - 1154