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Inductions of CaC2 on Sperm Morphology and Viability of the Albino Mice (Mus musculus)

Authors: Dike H. Ogbuagu, Etsede J. Oritsematosan


This work investigated possible inductions of CaC2, often misused by fruit vendors to stimulate artificial ripening, on mammalian sperm morphology and viability. Thirty isogenic strains of male albino mice, Mus musculus (age≈ 8weeks; weight= 32.52.0g) were acclimatized (ambient temperature 28.0±1.0°C) for 2 weeks and fed standard growers mash and water ad libutum. They were later exposed to graded toxicant concentrations (w/w) of 2.5000, 1.2500, 0.6250, and 0.3125% in 4 cages. A control cage was also established. After 5 weeks, 3 animals from each cage were sacrificed by cervical dislocation and the cauda epididymis excised. Sperm morphology and viability were determined by microscopic procedures. The ANOVA, means plots, Student’s t-test and variation plots were used to analyze data. The common abnormalities observed included Double Head, Pin Head, Knobbed Head, No Tail and With Hook. The higher toxicant concentrations induced significantly lower body weights [F(829.899) ˃ Fcrit(4.19)] and more abnormalities [F(26.52) ˃ Fcrit(4.00)] at P˂0.05. Sperm cells in the control setup were significantly more viable than those in the 0.625% (t=0.005) and 2.500% toxicant doses (t=0.018) at the 95% confidence limit. CaC2 appeared to induced morphological abnormalities and reduced viability in sperm cells of M. musculus.

Keywords: Artificial ripening, Calcium carbide, fruit vendors, sperm morphology, sperm viability.

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[1] M. Asif, “Physico-chemical Properties and Toxic Effect of Fruit Ripening against Calcium Carbide,” Annals of Tropical Medicine and Public Health, 2012, vol. 5, pp. 150-160.
[2] Consumers Association of Penang (CAP) (2014), “Ban the Use of Carbide to Ripen Fruits”, Retrieved July 20, 2014 from
[3] S.A. Fattah, and M.Y. Ali, “Carbide Ripened Fruits -A Recent Health Hazard”, Faridpur Medical College Journal, 2010, vol. 5. No. 2, p. 37.
[4] USA Department of Health, “Description of a Hazardous Substance”, Fact Sheet, USA: Trenton, NJ. 2000, pp. 1-4.
[5] H. Kjuus, A. Andersen, and S. Langard, “Incidence of Cancer among Workers Producing Calcium Carbide”, Porsgrunn and the Cancer Registry of Norway, Norway, 2007.
[6] W.S. Rahman, and M.B. Chowdhurry, “Artificial Ripening: What we are Eating”, Journal of Medicine, 2008, vol. 9, pp. 42-44.
[7] A.J. Dhembare, A.B. Gholap, and K. Vandana, “Bitter Truth About Artificial Ripener” Journal of Experimental Zoology India, 2011, vol. 14, no. 1, pp. 187-189.
[8] R.K. Aduloju, O.A. Otubanjo, and P.G.C. Odeigah, (2008) “An in vivo assay of the mutagenic potential of praziquantel (PZQ) using sperm head abnormality test”, Journal of Human Ecology, 2008, vol. 23, no. 1, pp. 59-63.
[9] A.A. Otitoloju, I.A. Obe, O.A. Adewale, O.A. Otubanjo, and V.O. Osunkalu, “Preliminary study on the induction of sperm head abnormalities in mice, Mus musculus, exposed to radiofrequency radiations from Global System for Mobile Communication base stations”, Bull. Environ. Contam. Toxicol., 2010, vol. 84, pp. 51-54.
[10] J. Chevrier, E. Dewailly, P. Ayotte, P. Mauriege, J.P. Despres, and A. Tremblay, “Body Weigh Los Increases Plasma and Adipose Tissue Concentrations of Potentially Toxic Pollutants in Obese Individuals” International Journal of Obesity, 2000, vol. 24, no. 10, pp. 1272 – 1278.
[11] O.A. Otubanjo, and A.A. Mosuro, “An in vivo evaluation of induction of abnormal sperm morphology by some anthelminthic drugs in mice.” Mutat. Res., 2001, vol. 497, pp. 131-138.
[12] A.A. Bakare, A.A., Mosuro, and O. Osibanjo, “An in vivo evaluation of induction of abnormal sperm morphology in mice by landfill leachates. Mutat Res, 2005, vol. 582, pp. 28-34.
[13] W.R. Bruce, and J.A. Heddle, “The mutagenic activity of 61 agents as determined by the micronucleus, Salmonella and sperm abnormality assays”, Canadian Journal of Genetics Cytology, 1979, vol. 21, pp. 319-334.
[14] P.G.C. Odeigah, “Sperm head abnormalities and dorminant lethal effects of formaldehyde in albino rats” Mutation Research, 1997, vol. 389, pp. 141-148.
[15] R.J. Aitken, “The Biology of Fertilization”, Advance in Experimental Medicine and Biology, 1997, vol. 424, pp. 29-291.
[16] M.G. Hull, “Infertility”, Practitioner, 1985, vol. 229, pp. 5-943.
[17] H.O. Adami, R. Bergstrom, and M. Mohner, “Testicular Cancer in Nine Northern European Countries. International Journal of Cancer, 1994, vol. 59, pp. 33-38.