Kinetic and Optimization Studies on Ethanol Production from Corn Flour
Studies on Simultaneous Saccharification and Fermentation (SSF) of corn flour, a major agricultural product as the substrate using starch digesting glucoamylase enzyme derived from Aspergillus niger and non starch digesting and sugar fermenting Saccharomyces cerevisiae in a batch fermentation. Experiments based on Central Composite Design (CCD) were conducted to study the effect of substrate concentration, pH, temperature, enzyme concentration on Ethanol Concentration and the above parameters were optimized using Response Surface Methodology (RSM). The optimum values of substrate concentration, pH, temperature and enzyme concentration were found to be 160 g/l, 5.5, 30°C and 50 IU respectively. The effect of inoculums age on ethanol concentration was also investigated. The corn flour solution equivalent to 16% initial starch concentration gave the highest ethanol concentration of 63.04 g/l after 48 h of fermentation at optimum conditions of pH and temperature. Monod model and Logistic model were used for growth kinetics and Leudeking – Piret model was used for product formation kinetics.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1329006Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3440
 J. Sainz, F. Pizarro, J.R. P'erez-Correa and E.A. Agosin, "Modeling of yeast metabolism and process dynamics in batch fermentation", Biotechnol.Bioeng., vol.81, pp. 818-828, 2003.
 M.A.D.Neves, T. Kimura, and N. Shimizu, "Production of Alcohol by Simultaneous Saccharification and Fermentation of Low Grade Wheat Flour", Brazilian Arch. Biol. Technol., vol. 49, pp. 481 - 190, 2006.
 H.Tanaka, H.Kurosawa, and H.Murakami,"Ethanolproduction from starch by a co-immobilized mixed culture system of Aspergillus awamori and Zymomonas mobilis", Biotechnol.Bioeng., vol.28, pp.1761-1768, 1986.
 S.W.Lee, T.Ebata, Y.C.Liu, and H.Tanaka, "Co-immobilization of three strains of microorganisms and its application in ethanol production from raw starch under unsterile conditions", J. Ferment. Bioeng., vol. 75, pp.36-42, 1993.
 J.C.Ogbonna, S.Tomiyama, Y.C. Liu, and H.Tanaka, "Efficient production of ethanol by cells immobilized in loofa (Lufa cylindrica) sponge", J. Ferment. Bioeng., vol. 84, pp.271-274, 1997.
 R.Amntka, and E.Gunasekaran, "Improved ethanol production by a mixed culture of Saccharomyces diastaticus and Zymomonas mobilis from liquefied cassava starch", Indian J. Microbiol., vol.40, pp.103- 107, 2000.
 S.K.Rhee, C.H.Lee, C.H.Kim, Z.Abidin, and M.H.Han, "Simultaneous sago starch hydrolysis and ethanol production by Zymomonas mobilis and Glucoamylase", Biotechol. Bioeng. Symp., vol.17, pp.481-493, 1986.
 Y. Nakamura, F. Kobayashi, M. Ohnaga, and T. Sawada, "Alcohol fermentation of starch by a genetic recombinant yeast having glucoamylase activity", Biotechnol. Bioeng., vol.53, pp. 21-25, 1997.
 F. Kobayashi, T. Sawada, Y. Nakamura, M. Ohnaga, M. Godliving, and T. Ushiyama, "Saccharification and alcohol fermentation in starch solution of steam-exploded potato", Appl. Biochem. Biotechnol., vol.69 pp.177-189, 1998.
 G.L. Miller, "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Anal. Chem., vol.31, pp.426-429,1959.
 S.Aiba, "Biochemical Engineering: Comprehensive text on fermentation of batch kinetics". In: Aiba S (Ed) Biochemical Engineering, 2nd Edn. Academic Press Inc, New York, 1973.
 J.E. Bailey, "Kinetics of substrate utilization, product formation and biomass production in cell cultures. In: Bailey J E (Ed) Biochemical Engineering Fundamentals, 3rd Edn. McGraw Hill Book Company, New York, 1986.