Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30982
Quantitative Characteristics of Rainbow Trout, Oncorhynchus Mykiss, Neo-Males (XX Genotype) and Super-Males (YY Genotype) Sperm

Authors: Radosław K. Kowalski, Beata Sarosiek, Wiesław Demianowicz, Jędrek Judek, Krzysztof Goryczko, Stefan Dobosz, Henryk Kuźmiński, Krystyna Demska-Zakęś, Igor Babiak, Jan Glogowski


Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in samples of stripped and testicular sperm obtained from XY and YY males, and testicular sperm of XX males. The motile spermatozoa, as measured by both subjective method and CASA, showed no differences between testicular sperm of XX males and stripped sperm of XY and YY males whereas testicular sperm of XY and YY males had significantly lower sperm motility. Result of protein densitometry showed similarities in protein profile between seminal plasma of XY and YY males and testicular fluids of XX males. Testis of XX males showed specific histological structures of cysts consists hypertrophied Sertoli cells.

Keywords: Fish, genotype, rainbow trout, sperm

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2559


[1] G. H. Aas, T. Refstic and B. Gjerde, "Evaluation of milt quality od Atlantic salmon". Aquaculture 95: 125-132, 1991.
[2] I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, P. Brzuzan and S. Ciesielski, "Androgenesis in rainbow trout using cryopreserved spermatozoa: the effect of processing and biological factors". Theriogenology 57(4): 1229-1249, 2002b.
[3] I. Babiak, S. Dobosz, K. Goryczko, H. Kuzminski, K. Goryczko, S. Ciesielski, P. Brzuzan, B. Urbanyi, A. Horvarth, F. Lahnsteiner and J. Piironen, "Failure of interspecies androgenesis in salmonids". J. Fish Biol. 61: 432-447, 2002a.
[4] K. Bieniarz, K. Goryczko, S. Dobosz and T. Grudniewski, "The effects of 17a-methylotestosterone on rainbow trout (Oncorhynchus mykiss)". Pol. Arch. Hydrobiol. 38: 295-301, 1991.
[5] R. Billard, "Utilisation d-un systeme tri-glycocolle pour tamponer le dilueur d-insemination pour truite" Bulletin Français de Pêche et Pisciculture 264: 102-112, 1977.
[6] R. Billard, J. Cosson, G. Perchec and O. Linhart, "Biology of sperm and artificial reproduction in carp". Aquaculture 129: 95-112, 1995.
[7] V. J. Bye and R. F. Lincoln, "Commercial methods for the control of sexual maturation in rainbow trout (Salmo gairdneri) ". Aquaculture 57: 299-310, 1986.
[8] D. Chourrout "Pressure-induced retention of second polar body and suppression of first cleavage in rainbow trout: production of all-triploids, all-tetraploids, and heterozygous and homozygous diploid gynogenetics". Aquaculture 36: 111-26, 1984.
[9] A. Ciereszko and K. Dabrowski, "Estimation of sperm concentration of rainbow trout, whitefish and yellow perch using spectrophotometric technique". Aquaculture 109: 367-373, 1993.
[10] A. Ciereszko and K. Dabrowski, "Relationship between biochemical constituents of fish sperm and fertility: the effect of short-term storage". Fish Physiol Biochem. 12: 357-367, 1994
[11] A. Ciereszko, B. Piros, K. Dabrowski, D. Kucharczyk, M. J. Łuczyński, S. Dobosz, and J. Glogowski, "Serine proteinase inhibitors of seminal plasma of teleost fish: distribution of activity, electrophoretic profiles and relation to proteinase inhibitors of blood". J.Fish Bio. 53: 1292- 1305, 1998.
[12] A. Ciereszko, T. Własow, S. Dobosz, K. Goryczko and J. Glogowski, "Blood cells in rainbow trout Oncorhynchus mykiss ilt: relation to milt collection method and sampling period". Theriogenology 62: 1353-1364,2004.
[13] G. J. Dietrich, R. Kowalski, M. Wojtczak, S. Dobosz and K. Goryczko, "Motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa in relation to sequential collection of milt, time of post mortem storage and anesthesia". Fish Physiol. Biochem. 31: 1-9, 2005b.
[14] G. J. Dietrich, A. Szpyrka, M. Wojtczak, S. Dobosz, K. Goryczko, Ł. Żakowski and A. Ciereszko, „Effects of UV irradiation and hydrogen peroxide on DNA fragmentation, motility and fertilizing ability of rainbow trout (Oncorhynchus mykiss) spermatozoa”. Theriogenology 64: 1809-1822, 2005a
[15] E.M. Donaldson, “Manipulation of reproduction in farmed fish.” Anim Reprod Sci 42: 381–392, 1996.
[16] C. Dreanno, M. Suquet, E. Desbruyeres, J. Cosson, H. Le Delliou, and R. Billard, “Effect of urine on sperm quality in turbot (Psetta maxima)”. Aquaculture 169(3–4): 247–262, 1998.
[17] K. Dziewulska and J. Domagała, “Histology of salmonid testis during maturation”. Reprod. Biol. 3(1): 47-61, 2003.
[18] J. L. Fitzpatrick, J. C. Henry, N. R. Liley and R. H. Devlin, “Sperm characteristicks and fertilization succes of masculinized coho salmon (Oncorhynchus kisutch)”. Aquaculture 249: 459-468, 2005.
[19] M. J. G. Gage, C. P. Macfarlane, S. Yeates, R. G. Ward, J. B. Searle and G. A. Parker, “Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success”. Curr. Biol. 14: 44–47, 2004.
[20] J. F. Galas, A. Hejmej, J. Glogowski and B. Bilińska, “Morphological and functional alterations in testis and efferent ducts of homogametic rainbow trout Oncorhynchus mykiss Walbaum”. Ann N Y Acad Sci 1163: 398–401, 2009.
[21] A. J. Geffen and J. P. Evans, “Sperm traits and fertilization success of male and sex-reversed female rainbow trout (Oncorhynchus mykiss)”. Aquaculture 182: 61-72, 2000.
[22] S.E. Hartley, “The chromosomes of Salmonid fishes”. Biol. Rev. 62: 197-214, 1987.
[23] D. J. Hoysak and N. R. Liley. “Fertilization dynamics in sockeye salmon and a comparison of sperm from alternative male phenotypes”. J. Fish Biol. 58: 1286-1300, 2000.
[24] D. E. Kime, K. J. W. Van Look, B. G. McAllister, G. Huyskens, E. Rurangwa and F. Ollevier , “Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish”. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 130: 425–433, 2001.
[25] K. Kinnberg, B. Korsgaard, P. Bjerregaard and A. Jespersen, “Effects of nonylphenol and 17β-estradiol on vitellogenin synthesis and testis morphology in male platyfish Xiphophorus maculates”. J. Exp. Biol. 203: 171-181, 2000.
[26] M. Koldras, M. Loir, G. Maisse and F. Le Gae, “Study of the composition of the seminal fluid and of sperm motility along genital tract, during a spawning season, in the rainbow trout (Oncorhynchus mykiss) “. Aquat. Living Resour. 9(4): 337-345, 1996.
[27] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner, „Physiological and biochemical determination of rainbow trout, Oncorhynchus mykiss, sperm quality for cryopreservation”. J. Appl. Ichthyol. 6(4): 47-73, 1996.
[28] F. Lahnsteiner, B. Berger, T. Weismann and R. A. Patzner, „Determination of sperm quality of the rainbow trout, Oncorhynchus mykiss, by sperm motility, seminal plasma parameters, and spermatozoal metabolism”. Aquaculture 163: 163-181, 1998.
[29] F. Lahnsteiner, N. Mansour and B. Berger. “Seminal plasma proteins prolong the viability of rainbow trout (Oncorynchus mykiss) spermatozoa”. Theriogenology 62: 801-808, 2004.
[30] F. Lahnsteiner, R. A. Patzner and T. Weismann, „Energy resources of spermatozoa of the rainbow trout Oncorhynchus mykiss (Pisces, Teleostei)”. Reprod. Nutr. Dev. 33: 349-360, 1993b.
[31] F. Lahnsteiner, R. Patzner and T. Weismann, “The spermatic ducts of Salmonid fishes (Salmonidae, Teleostei). Morphology, histochemistry and composition of the secretion”. J. Fish Biol. 42: 79-93, 1993a.
[32] F. Lahnsteiner, R. Patzner and T. Weismann, “The testicular main ducts and the spermatic ducts in some cyprinid fishes-II. Composition of the seminal fluid”. J. Fish Biol. 44: 459-467, 1994.
[33] N. Mansour, A. Ramoun and F. Lahnsteiner, “Quality of testicular sperm of the African catfish Clarias gariepinus (Burchell, 1822) and its relationship with fertilization and hatching success”. Aquacult. Res. 36: 1422-1428, 2005.
[34] S. Morisawa and M. Morisawa, “Acquisition of potential for sperm motility in rainbow trout and chum salmon”. J. Exp. Biol. 126: 89-96, 1986.
[35] H. Ohta, T. Unuma, M. Yoshioka and M. Kashiwagi, “Effects of bicarbonate ions and pH on acquisition and maintenance of potential for motility in ayu, Plecoglossus altivelis Temminck et Schlegel (Osmeridae), spermatozoa”. Aquacult. Res. 32: 385-392, 2001.
[36] A. Pecio, F. Lahnsteiner and J. Rafiński, “Ultrastucture of the epithelial cells in the aspermatogenic part of the testis in Mimagoniates barberi (Teleostei: Characidae: Glandulocaudinae) and the role of their secretions in spermatozeugmata formation”. Ann. Anat. 183: 427-435, 2001.
[37] G. Perchec J. , Cosson, F. Andre and R. Billard, “Degradation of the quality of carp sperm by urine contamination during stripping”. Aquaculture 129: 135-136, 1995.
[38] K. Ravinder, K. Nasaruddin, K. C. Majumdar and S. Shivaji, ”Computerized analysis of motility, motility patterns and motility parameters of spermatozoa of carp following short-term storage of sperm”. J. Fish Biol. 50: 1309-1328, 1997.
[39] H. Schagger and G. von Jagow, “Tricine-sodium dodecyl sulfate- polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa”. Anal. Biochem. 166: 368-379, 1987.
[40] A. P. Scott and S. M. Baynes, “A review of the biology, handling and storage of salmonid spermatozoa”. J. Fish Biol. 17: 707-739, 1980.
[41] S. Shivaji, J. Peedicayil andD. L. Girija, “Analysis of the motility parameters of in vitro hyperactivated hamster spermatozoa”. Mol. Reprod. Dev. 42: 233-247, 1995