Search results for: Rosli A. Bakar

Authors: M. M. Rahman, Thamir K. Ibrahim, M. Y. Taib, M. M. Noor, Rosli A. Bakar

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Regenerative gas turbine engine cycle is presented that yields higher cycle efficiencies than simple cycle operating under the same conditions. The power output, efficiency and specific fuel consumption are simulated with respect to operating conditions. The analytical formulae about the relation to determine the thermal efficiency are derived taking into account the effected operation conditions (ambient temperature, compression ratio, regenerator effectiveness, compressor efficiency, turbine efficiency and turbine inlet temperature). Model calculations for a wide range of parameters are presented, as are comparisons with simple gas turbine cycle. The power output and thermal efficiency are found to be increasing with the regenerative effectiveness, and the compressor and turbine efficiencies. The efficiency increased with increase the compression ratio to 5, then efficiency decreased with increased compression ratio, but in simple cycle the thermal efficiency always increase with increased in compression ratio. The increased in ambient temperature caused decreased thermal efficiency, but the increased in turbine inlet temperature increase thermal efficiency.

Keywords: Gas turbine, power plant, thermal analysis, regeneration

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Authors: Md. Ashikur Rahman Khan, M. M. Rahman, K. Kadirgama, M.A. Maleque, Rosli A. Bakar

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Conventionally the selection of parameters depends intensely on the operator-s experience or conservative technological data provided by the EDM equipment manufacturers that assign inconsistent machining performance. The parameter settings given by the manufacturers are only relevant with common steel grades. A single parameter change influences the process in a complex way. Hence, the present research proposes artificial neural network (ANN) models for the prediction of surface roughness on first commenced Ti-15-3 alloy in electrical discharge machining (EDM) process. The proposed models use peak current, pulse on time, pulse off time and servo voltage as input parameters. Multilayer perceptron (MLP) with three hidden layer feedforward networks are applied. An assessment is carried out with the models of distinct hidden layer. Training of the models is performed with data from an extensive series of experiments utilizing copper electrode as positive polarity. The predictions based on the above developed models have been verified with another set of experiments and are found to be in good agreement with the experimental results. Beside this they can be exercised as precious tools for the process planning for EDM.

Keywords: Ti-15l-3, surface roughness, copper, positive polarity, multi-layered perceptron.

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Authors: Mohammed Kamil, M. M. Rahman, Rosli A. Bakar

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Modeling of hydrogen fueled engine (H₂ICE) injection system is a very important tool that can be used for explaining or predicting the effect of advanced injection strategies on combustion and emissions. In this paper, a common rail injection system (CRIS) is proposed for 4-strokes 4-cylinders hydrogen fueled engine with port injection feeding system (PIH₂ICE). For this system, a numerical one-dimensional gas dynamic model is developed considering single injection event for each injector per a cycle. One-dimensional flow equations in conservation form are used to simulate wave propagation phenomenon throughout the CR (accumulator). Using this model, the effect of common rail on the injection system characteristics is clarified. These characteristics include: rail pressure, sound velocity, rail mass flow rate, injected mass flow rate and pressure drop across injectors. The interaction effects of operational conditions (engine speed and rail pressure) and geometrical features (injector hole diameter) are illustrated; and the required compromised solutions are highlighted. The CRIS is shown to be a promising enhancement for PIH₂ICE.

Keywords: Common rail, hydrogen engine, port injection, wave propagation.

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Authors: M. M. Rahman, Md. Ashikur Rahman Khan, K. Kadirgama M. M. Noor, Rosli A. Bakar

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This paper deals optimized model to investigate the effects of peak current, pulse on time and pulse off time in EDM performance on material removal rate of titanium alloy utilizing copper tungsten as electrode and positive polarity of the electrode. The experiments are carried out on Ti6Al4V. Experiments were conducted by varying the peak current, pulse on time and pulse off time. A mathematical model is developed to correlate the influences of these variables and material removal rate of workpiece. Design of experiments (DOE) method and response surface methodology (RSM) techniques are implemented. The validity test of the fit and adequacy of the proposed models has been carried out through analysis of variance (ANOVA). The obtained results evidence that as the material removal rate increases as peak current and pulse on time increases. The effect of pulse off time on MRR changes with peak ampere. The optimum machining conditions in favor of material removal rate are verified and compared. The optimum machining conditions in favor of material removal rate are estimated and verified with proposed optimized results. It is observed that the developed model is within the limits of the agreeable error (about 4%) when compared to experimental results. This result leads to desirable material removal rate and economical industrial machining to optimize the input parameters.

Keywords: Ti-6Al-4V, material removal rate, copper tungsten, positive polarity, RSM.

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Authors: Abu Bakar Sedek Abdul Jamak, Saridan Abu Bakar, Zulkipli Ghazali, Roselind Wan

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The majority of micro-entrepreneurs in Malaysia operate very small-scaled business activities such as food stalls, burger stalls, night market hawkers, grocery stores, constructions, rubber and oil palm small holders, and other agro-based services and activities. Why are they venturing into entrepreneurship - is it for survival, out of interest or due to encouragement and assistance from the local government? And why is it that some micro-entrepreneurs are lagging behind in entrepreneurship, and what do they need to rectify this situation so that they are able to progress further? Furthermore, what are the skills that the micro entrepreneurs should developed to transform them into successful micro-enterprises and become small and medium-sized enterprises (SME)? This paper proposes a 7-Step approach that can serve as a basis for identification of critical entrepreneurial success factors that enable policy makers, practitioners, consultants, training managers and other agencies in developing tools to assist micro business owners. This paper also highlights the experience of one of the successful companies in Malaysia that has transformed from micro-enterprise to become a large organization in less than 10 years.

Keywords: Entrepreneurship, Micro-entrepreneurs, Transformation, Customers

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Authors: K.M.Goh, M.M.Mokji, S.A.R. Abu-Bakar

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Speeded-Up Robust Feature (SURF) is commonly used for feature matching in stereovision because of their robustness towards scale changes and rotational changes. However, SURF feature cannot cope with large viewpoint changes or skew distortion. This paper introduces a method which can help to improve the wide baseline-s matching performance in term of accuracy by rectifying the image using two vanishing points. Simplified orientation correction was used to remove the false matching..

Keywords: Affine, orientation, projective, SURF.

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Authors: Abu Bakar Munir, Siti Hajar Mohd Yasin, Firdaus Muhammad-Sukki

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This paper seeks to analyse the benefits of big data and more importantly the challenges it pose to the subject of privacy and data protection. First, the nature of big data will be briefly deliberated before presenting the potential of big data in the present days. Afterwards, the issue of privacy and data protection is highlighted before discussing the challenges of implementing this issue in big data. In conclusion, the paper will put forward the debate on the adequacy of the existing legal framework in protecting personal data in the era of big data.

Keywords: Big data, data protection, information, privacy.

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Authors: Y. Rosli, A. Husna AH, M. Nurhayati

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This research was carried out to determine the possible effects of low electromagnetic field (EMF) exposure to the developing mice fetuses. Pregnant mice were exposed to EMF exposure at 0mT (sham) and 1.2 mT for six hours per session, carried out on gestation day 3, 6, 9, 12 and 15. Samples from the stillborn offspring were observed for morphological defects. The heart didn-t show progressive cellular damage, the lungs were congested and emphysemics. The bones were in advance stage of hypertrophy. Spectrums of morphological defects were observed over 70% of the surviving offspring. These results indicate that even at lower exposure to low EMF, is enough to induce morphological defects in prenatal mice.

Keywords: bone hypertrophy, EMF, low electromagnetic field.

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Authors: Saunah Zainon, Ruhaya Atan, Yap Bee Wah, Zarina Abu Bakar

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The primary objective of this study is to test whether there is any difference in performance between funded and nonfunded registered charity organizations. In this study, performance as the dependent variable is measured using total donations. Using a sample of 101 charity organizations registered with the Registry of Society, analysis of variance (ANOVA) results indicate that there is a difference in financial performance between funded and non-funded charity organizations. The study provides empirical evidence to resource providers and the policy makers in scrutinizing the decision to disburse their funds and resources to these charity organizations.

Keywords: charity organizations, donations, funded, non-funded

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Authors: Nabilah Filzah Mohd Radzuan, Zalinda Othman, Azuraliza Abu Bakar, Abdul Razak Hamdan

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Uncertain data is believed to be an important issue in building up a prediction model. The main objective in the time series uncertainty analysis is to formulate uncertain data in order to gain knowledge and fit low dimensional model prior to a prediction task. This paper discusses the performance of a number of techniques in dealing with uncertain data specifically those which solve uncertain data condition by minimizing the loss of compression properties.

Keywords: Compression properties, uncertainty, uncertain time series, mining technique, weather prediction.

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Authors: Abd Mutalib Embong, Azelin M Noor, Razol Mahari M Ali, Zulqarnain Abu Bakar, Abdur- Rahman Mohamed Amin

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At the time where electronic books, or e-Books, offer students a fun way of learning , teachers who are used to the paper text books may find it as a new challenge to use it as a part of learning process. Precisely, there are various types of e-Books available to suit students- knowledge, characteristics, abilities, and interests. The paper discusses teachers- perceptions on the use of ebooks as a paper text book in the classroom. A survey was conducted on 72 teachers who use e-books as textbooks. It was discovered that a majority of these teachers had good perceptions on the use of ebooks. However, they had little problems using the devices. It can be overcome with some strategies and a suggested framework.

Keywords: Classroom, E-books, perception, teacher.

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Authors: Seyedeh Belin Tavakoly Sany, Majid Rezayi, Rosli Hashim, Aishah Salleh, Omid Safari

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The purpose of this paper is to describe the main characteristics of macroinvertebrate species in response to environmental forcing factors. Overall, 23 species of Mollusca, 4 species of Arthropods, 3 species of Echinodermata and 3 species of Annelida were identified at the 9 sampling stations during four sampling periods. Individual species of Mollusca constituted 36.4% of the total abundance, followed by Arthropods (27.01%), Annelida (34.3%) and Echinodermata (2.4%). The results of Kruskal-Wallis test indicated that a significant difference (p <0.05) in the abundance, richness and diversity of the macro-benthic community in different stations. The correlation analysis revealed that anthropogenic pollution and natural variability caused by these variations in spatial scales.

Keywords: Benthic invertebrates, Diversity, Malaysia, West Port.

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Authors: M. W. Yip, H. H. Ng, S. Din, N. Abu Bakar

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This paper reviewed the relationships between the Knowledge Management (KM) activities and its perceived benefits in the knowledge based organisations. KM activities include: knowledge identification, knowledge acquisition, knowledge application, knowledge sharing, knowledge creation and knowledge preservation. And the perceived benefits of KM are fast customer responsiveness, operation excellence and high innovative intensity.  Based on the above review, a conceptual framework for KM implementation in retail business organisations has been proposed. Finally the paper forwarded some limitations of the framework and based on which, directions for future research had been suggested.

Keywords: Knowledge Management, Knowledge Management Activities, Retail Business, Knowledge Economy.

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Authors: Ridzuan Ramli, Norhafzan Junadi, Mohammad D.H. Beg, Rosli M. Yunus

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In this study, microcrystalline cellulose (MCC) was extracted from oil palm empty fruit bunch (EFB) cellulose which was earlier isolated from oil palm EFB fibre. In order to isolate the cellulose, the chlorination method was carried out. Then, the MCC was prepared by simultaneous ultrasonic and alkali treatment from the isolated α-cellulose. Based on mass balance calculation, the yields for MCC obtained from EFB was 44%. For fiber characterization, it is observed that the chemical composition of the hemicellulose and lignin for all samples decreased while composition for cellulose increased. The structural property of the MCC was studied by X-ray diffraction (XRD) method and the result shows that the MCC produced is a cellulose-I polymorph, with 73% crystallinity.

Keywords: Oil palm empty fruit bunch, microcrystalline cellulose, ultrasonic, alkali treatment, X-ray diffraction.

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Authors: Justin Leo Cheang Loong, Khazaimatol S Subari, Rosli Besar, Muhammad Kamil Abdullah

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In this paper, a novel method for a biometric system based on the ECG signal is proposed, using spectral coefficients computed through linear predictive coding (LPC). ECG biometric systems have traditionally incorporated characteristics of fiducial points of the ECG signal as the feature set. These systems have been shown to contain loopholes and thus a non-fiducial system allows for tighter security. In the proposed system, incorporating non-fiducial features from the LPC spectrum produced a segment and subject recognition rate of 99.52% and 100% respectively. The recognition rates outperformed the biometric system that is based on the wavelet packet decomposition (WPD) algorithm in terms of recognition rates and computation time. This allows for LPC to be used in a practical ECG biometric system that requires fast, stringent and accurate recognition.

Keywords: biometric, ecg, linear predictive coding, wavelet packet decomposition

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Authors: Syahanim Mohd Salleh, Zaihosnita Hood, Hairulliza Mohd Judi, Marini Abu Bakar

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CFlow is a flow chart software, it contains facilities to draw and evaluate a flow chart. A flow chart evaluation applies a simulation method to enable presentation of work flow in a flow chart solution. Flow chart simulation of CFlow is executed by manipulating the CFlow data file which is saved in a graphical vector format. These text-based data are organised by using a data classification technic based on a Library classification-scheme. This paper describes the file format for flow chart simulation software of CFlow.

Keywords: CFlow, flow chart, file format.

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Authors: R. Ridzuan, M. D. H. Beg, M. Y. Rosli, M. H. Rohaya, A. A. Astimar S. Samahani, I. Zawawi

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Alkali treated oil palm empty fruit bunch (EFB) fibres (TEFBF) and untreated EFBF fibers (UEFBF) were incorporated in polypropylene (PP) with and without malic anhydride grafted PP (MAPP) and magnesium hydroxide as flame retardant (FR) to produce TEFBF-PP and UEFBF-PP composites by the melt casting method. The composites were characterized by mechanical and burning tests along with a scanning electron microscope and Fourier transform infrared spectroscopy. The significant improvement in flexural modulus (133%) and flame retardant property (60%) of TEFBF-PP composite with MAPP and FR is observed. The improved mechanical property is discussed by the development of encapsulated textures.

Keywords: Empty fruit bunch fibers, polypropylene, mechanical property, flame retardant.

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Authors: Abdurahman H. Nour, Rosli M. Yunus, Azhary. H. Nour

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The mechanism of microwave heating is essentially that of dielectric heating. After exposing the emulsion to the microwave Electromagnetic (EM) field, molecular rotation and ionic conduction due to the penetration of (EM) into the emulsion are responsible for the internal heating. To determine the capability of microwave technology in demulsification of crude oil emulsions, microwave demulsification method was applied in a 50-50 % and 20- 80 % water-in-oil emulsions with microwave exposure time varied from 20-180 sec. Transient temperature profiles of water-in-oil emulsions inside a cylindrical container were measured. The temperature rise at a given location was almost horizontal (linear). The average rates of temperature increase of 50-50 % and 20-80 % water-in-oil emulsions are 0.351 and 0.437 oC/sec, respectively. The rate of temperature increase of emulsions decreased at higher temperature due to decreasing dielectric loss of water. These results indicate that microwave demulsification of water-in-oil emulsions does not require chemical additions. Microwave has the potential to be used as an alternative way in the demulsification process.

Keywords: Demulsification, temperature profile, emulsion.Microwave heating, dielectric, volume rate.

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Authors: Amir Vahid Dastjerdi, Kamalrulnizam Abu Bakar

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The first generation of Mobile Agents based Intrusion Detection System just had two components namely data collection and single centralized analyzer. The disadvantage of this type of intrusion detection is if connection to the analyzer fails, the entire system will become useless. In this work, we propose novel hybrid model for Mobile Agent based Distributed Intrusion Detection System to overcome the current problem. The proposed model has new features such as robustness, capability of detecting intrusion against the IDS itself and capability of updating itself to detect new pattern of intrusions. In addition, our proposed model is also capable of tackling some of the weaknesses of centralized Intrusion Detection System models.

Keywords: Distributed Intrusion Detection System, Mobile Agents, Network Security.

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Authors: W. I. Wan Rosli, A. R. Nurhanan, M. A. Solihah, S. S. J. Mohsin

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The proximate composition, physical traits and sensory properties of beef and chicken patties incorporated with various level of dried cornsilk (Maydis stigma) were studied. The beef and chicken patties were formulated with either 2%, 4% or 6% of cornsilk. Both cooked beef and chicken patties incorporated with 6% cornsilk recorded the highest protein concentration at 23.3% and 28.42%, respectively. Both cooked beef and chicken patties containing 6% cornsilk significantly recorded the lowest concentration of fat at 11.4% and 14.60%, respectively. Beef and chicken patties formulated with 6% cornsilk recorded the highest cooking yield at 80.13% and 83.03% compared to other treatments. The inclusion of cornsilk did not change the sensory properties and consumer acceptability of cornsilk-based beef and chicken patties. Cornsilk fibre has been effective in improving cooking yield, moisture and fat retention of beef and chicken patties

Keywords: cornsilk, beef and chicken patty, proximatecomposition, sensory evaluation.

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Authors: Nabilah Filzah Mohd Radzuan, Andi Putra, Zalinda Othman, Azuraliza Abu Bakar, Abdul Razak Hamdan

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Precipitation forecast is important in avoid incident of natural disaster which can cause loss in involved area. This review paper involves three techniques from artificial intelligence namely logistic regression, decisions tree, and random forest which used in making precipitation forecast. These combination techniques through VAR model in finding advantages and strength for every technique in forecast process. Data contains variables from rain domain. Adaptation of artificial intelligence techniques involved on rain domain enables the process to be easier and systematic for precipitation forecast.

Keywords: Logistic regression, decisions tree, random forest, VAR model.

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Authors: Razib M. Othman, Safaai Deris, Rosli M. Illias

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Gene Ontology terms have been actively used to annotate various protein sets. SWISS-PROT, TrEMBL, and InterPro are protein databases that are annotated according to the Gene Ontology terms. However, direct implementation of the Gene Ontology terms for annotation of anonymous protein sequences is not easy, especially for species not commonly represented in biological databases. UTMGO is developed as a tool that allows the user to quickly and easily search for a group of semantically related Gene Ontology terms. The applicability of the UTMGO is demonstrated by applying it to annotation of anonymous protein sequence. The extended UTMGO uses the Gene Ontology terms together with protein sequences associated with the terms to perform the annotation task. GOPET, GOtcha, GoFigure, and JAFA are used to compare the performance of the extended UTMGO.

Keywords: Anonymous protein sequence, Gene Ontology, Protein sequence annotation, Protein sequence alignment

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Authors: Kok, M.O., Omar-Fauzee, M.S., Rosli, M.H.

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This study investigated the relationship between exercise imagery use and level of physical activity within a wide range of exercisers in Klang valley, Malaysia. One hundred and twenty four respondents (Mage = 28.92, SD = 9.34) completed two sets of questionnaires (Exercise Imagery Inventory and Leisure-Time Exercise Questionnaire) that measure the use of imagery and exercise frequency of participants. From the result obtained, exercise imagery is found to be significantly correlated to level of physical activity. Besides that, variables such as gender, age and ethnicity that may affect the use of imagery and exercise frequency were also being assessed in this study. Among all variables, only ethnicity showed significant difference in level of physical activity (p < 0.05). Findings in this study suggest that further investigation should be done on other variables such as socioeconomic, educational level, and selfefficacy that may affect the imagery use and frequency of physical activity among exercisers.

Keywords: Physical activity, exercise imagery, ExerciseImagery Inventory, Leisure-Time Exercise Questionnaire

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Authors: Razib M. Othman, Safaai Deris, Rosli M. Illias, Zalmiyah Zakaria, Saberi M. Mohamad

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Nowadays, Gene Ontology has been used widely by many researchers for biological data mining and information retrieval, integration of biological databases, finding genes, and incorporating knowledge in the Gene Ontology for gene clustering. However, the increase in size of the Gene Ontology has caused problems in maintaining and processing them. One way to obtain their accessibility is by clustering them into fragmented groups. Clustering the Gene Ontology is a difficult combinatorial problem and can be modeled as a graph partitioning problem. Additionally, deciding the number k of clusters to use is not easily perceived and is a hard algorithmic problem. Therefore, an approach for solving the automatic clustering of the Gene Ontology is proposed by incorporating cohesion-and-coupling metric into a hybrid algorithm consisting of a genetic algorithm and a split-and-merge algorithm. Experimental results and an example of modularized Gene Ontology in RDF/XML format are given to illustrate the effectiveness of the algorithm.

Keywords: Automatic clustering, cohesion-and-coupling metric, gene ontology; genetic algorithm, split-and-merge algorithm.

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Authors: Chen May Oh, Nadzrah Abu Bakar

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This case study investigates the areas of self-reflection through the written content of four university students’ blogs. The study was undertaken to explore the categories of self-reflection in relation to the use of blogs. Data collection methods included downloading students’ blog entries and recording individual interviews to further support the data. Data was analyzed using computer assisted qualitative data analysis software, Nvivo, to categories and code the data. The categories of self-reflection revealed in the findings showed that university students used blogs to reflect on (1) life in varsity, (2) emotions and feelings, (3) various relationships, (4) personal growth, (5) spirituality, (6) health conditions, (7) busyness with daily chores, (8) gifts for people and themselves and (9) personal interests. Overall, all four of the students had positive experiences and felt satisfied using blogs for self-reflection.

Keywords: Blogging, personal growth, self-reflection, university students.

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Authors: Y. T. Boon, M. N. Naim, R. Zakaria, N. F. Abu Bakar, N. Ahmad, I. W. Lenggoro

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In this study, encapsulation of agarwood oil with non-ionic surfactant, Tween 80 was prepared at critical micelle concentration of 0.0167 % v/v to produce the most stable nano-emulsion in aqueous. The encapsulation has minimized the bioactive compounds degradation in various pH conditions thus prolong their shelf life and maintained its initial oil grade. The oil grading of the prepared samples were conducted using the gel electrophoresis instead of using common analytical industrial grading such as gas chromatography- mass spectrometry (GC- MS). The grading method was chosen due to their unique zeta potential value after the encapsulation process. This paper demonstrates the feasibility of applying the electrophoresis principles to separate the encapsulated agarwood oil or grading of the emulsified agarwood oil. The results indicated that the grading process are potential to be further investigate based on their droplet size and zeta potential value at various pH condition when the droplet were migrate through polyacrylamide gel.

Keywords: Electrophoretic mobility, essential oil, nanoemulsion, polyacrylamide gel electrophoresis, Tween 80, zeta potential.

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Authors: Seyedeh Belin Tavakoly Sany, H. Rosli, R. Majid, S. Aishah

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In this study, water monitoring was performed from Nov. 2012 to Oct. 2013 to assess water quality and evaluate the spatial and temporal distribution of physicochemical and biological variables in water. Water samples were collected from 10 coastal water stations of West Port. In the case of water-quality assessment, multi-metric indices and operational indicators have been proposed to classify the trophic status at different stations. The trophic level of West Port coastal water ranges from eutrophic to hypertrophic. Chl-a concentration was used to estimate the biological response of phytoplankton biomass and indicated eutrophic conditions in West Port and mesotrophic conditions at the control site. During the study period, no eutrophication events or secondary symptoms occurred, which may be related to hydrodynamic turbulence and water exchange, which prevent the development of eutrophic conditions in the West Port.

Keywords: Water quality, multi-metric indices, operational indicator, Malaysia, West Port.

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Authors: Noor Suhana Sulaiman, Rohani Abu Bakar, Norrozila Sulaiman

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Intrusion Detection System is significant in network security. It detects and identifies intrusion behavior or intrusion attempts in a computer system by monitoring and analyzing the network packets in real time. In the recent year, intelligent algorithms applied in the intrusion detection system (IDS) have been an increasing concern with the rapid growth of the network security. IDS data deals with a huge amount of data which contains irrelevant and redundant features causing slow training and testing process, higher resource consumption as well as poor detection rate. Since the amount of audit data that an IDS needs to examine is very large even for a small network, classification by hand is impossible. Hence, the primary objective of this review is to review the techniques prior to classification process suit to IDS data.

Keywords: Intrusion Detection System, security, soft computing, classification.

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Authors: Ahmed K. Hasan, A. A. Zaidan, Anas Majeed, B. B. Zaidan, Rosli Salleh, Omar Zakaria, Ali Zuheir

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Wireless mesh networks based on IEEE 802.11 technology are a scalable and efficient solution for next generation wireless networking to provide wide-area wideband internet access to a significant number of users. The deployment of these wireless mesh networks may be within different authorities and without any planning, they are potentially overlapped partially or completely in the same service area. The aim of the proposed model is design a new model to Enhancement Throughput of Unplanned Wireless Mesh Networks Deployment Using Partitioning Hierarchical Cluster (PHC), the unplanned deployment of WMNs are determinates there performance. We use throughput optimization approach to model the unplanned WMNs deployment problem based on partitioning hierarchical cluster (PHC) based architecture, in this paper the researcher used bridge node by allowing interworking traffic between these WMNs as solution for performance degradation.

Keywords: Wireless Mesh Networks, 802.11s Internetworking, partitioning Hierarchical Cluste.

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Authors: P. Hashim, M. S. Mohd Ridzwan, J. Bakar

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Collagen was isolated from chicken feet by using papain and pepsin enzymes in acetic acid solution at 4°C for 24h with a yield of 18.16% and 22.94% by dry weight, respectively. Chemical composition and characteristics of chicken feet collagen such as amino acid composition, SDS-PAGE patterns, FTIR spectra and thermal properties were evaluated. The chicken feet collagen is rich in the amino acids glycine, glutamic acid, proline and hydroxyproline. Electrophoresis pattern demonstrated two distinct α-chains (α1 and α2) and β chain, indicating that type I collagen is a major component of chicken feet collagen. The thermal stability of collagen isolated by papain and pepsin revealed stable denaturation temperatures of 48.40 and 53.35°C, respectively. The FTIR spectra of both collagens were similar with amide regions in A, B, I, II and III. The study demonstrated that chicken feet collagen using papain isolation method is possible as commercial alternative ingredient. 

Keywords: Chicken feet, collagen, papain, pepsin.

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