Search results for: Fault Tolerant Software

Authors: Su Myat Marlar Soe, May Paing Paing Zaw

Abstract:

It has been defined that the “network is the system". This implies providing levels of service, reliability, predictability and availability that are commensurate with or better than those that individual computers provide today. To provide this requires integrated network management for interconnected networks of heterogeneous devices covering both the local campus. In this paper we are addressing a framework to effectively deal with this issue. It consists of components and interactions between them which are required to perform the service fault management. A real-world scenario is used to derive the requirements which have been applied to the component identification. An analysis of existing frameworks and approaches with respect to their applicability to the framework is also carried out.

Keywords: To diagnose the possible network faults by using thepredetermined rules.

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Authors: M. Tehranizadeh, E. Shoushtari Rezvani

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Seismic performance of steel moment-resisting frame structures is investigated considering nonlinear soil-structure interaction (SSI) effects. 10-, 15-, and 20-story planar building frames with aspect ratio of 3 are designed in accordance with current building codes. Inelastic seismic demands of the superstructure are considered using concentrated plasticity model. The raft foundation system is designed for different soil types. Beam-on-nonlinear Winkler foundation (BNWF) is used to represent dynamic impedance of the underlying soil. Two sets of pulse-like as well as no-pulse near-fault earthquakes are used as input ground motions. The results show that the reduction in drift demands due to nonlinear SSI is characterized by a more uniform distribution pattern along the height when compared to the fixed-base and linear SSI condition. It is also concluded that beneficial effects of nonlinear SSI on displacement demands is more significant in case of pulse-like ground motions and performance level of the steel moment-resisting frames can be enhanced.

Keywords: Soil-structure interaction, uplifting, soil plasticity, near-fault earthquake, tall building.

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Authors: Ioannis Binas, Marios Moschakis

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Electrical faults in transmission and distribution networks can have great impact on the electrical equipment used. Fault effects depend on the characteristics of the fault as well as the network itself. It is important to anticipate the network’s behavior during faults when planning a new equipment installation, as well as troubleshooting. Moreover, working backwards, we could be able to estimate the characteristics of the fault when checking the perceived effects. Different transformer winding connections dominantly used in the Greek power transfer and distribution networks and the effects of 1-phase to neutral, phase-to-phase, 2-phases to neutral and 3-phase faults on different locations of the network were simulated in order to present voltage sag characteristics. The study was performed on a generic network with three steps down transformers on two voltage level buses (one 150 kV/20 kV transformer and two 20 kV/0.4 kV). We found that during faults, there are significant changes both on voltage magnitudes and on phase angles. The simulations and short-circuit analysis were performed using the PSCAD simulation package. This paper presents voltage characteristics calculated for the simulated network, with different approaches on the transformer winding connections during symmetrical and asymmetrical faults on various locations.

Keywords: Phase angle shift, power quality, transformer winding connections, voltage sag propagation.

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Authors: Zhaopeng Xuan, Yuan Bian, C. Cailleaux, Jing Qin, S. Traore

Abstract:

Traditional software engineering allows engineers to propose to their clients multiple specialized software distributions assembled from a shared set of software assets. The management of these assets however requires a trade-off between client satisfaction and software engineering process. Clients have more and more difficult to find a distribution or components based on their needs from all of distributed repositories.

This paper proposes a software engineering for a user-driven software product line in which engineers define a Feature Model but users drive the actual software distribution on demand. This approach makes the user become final actor as a release manager in software engineering process, increasing user product satisfaction and simplifying user operations to find required components. In addition, it provides a way for engineers to manage and assembly large software families.

As a proof of concept, a user-driven software product line is implemented for Eclipse, an integrated development environment. An Eclipse feature model is defined, which is exposed to users on a cloud-based built platform from which clients can download individualized Eclipse distributions.

Keywords: Software Product Line, Model-driven Development, Reverse Engineering and Refactoring, Agile Method

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Authors: Raúl González Muñoz, Essam Shehab, Martin Weinitzke, Chris Fowler, Paul Baguley

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Software applications have become crucial to the aerospace industry, providing a wide range of functionalities and capabilities used during the design, manufacturing and support of aircraft. However, as this criticality increases, so too does the risk for business operations when facing a software failure. Hence, there is a need for new methodologies to be developed to support aerospace companies in effectively managing their software portfolios, avoiding the hazards of business disruption and additional costs. This paper aims to provide a definition of operational software maturity, and how this can be used to assess software operational behaviour, as well as a view on the different aspects that drive software maturity within the aerospace industry. The key research question addressed is, how can operational software maturity monitoring assist the aerospace industry in effectively managing large software portfolios? This question has been addressed by conducting an in depth review of current literature, by working closely with aerospace professionals and by running an industry case study within a major aircraft manufacturer. The results are a software maturity model composed of a set of drivers and a prototype tool used for the testing and validation of the research findings. By utilising these methodologies to assess the operational maturity of software applications in aerospace, benefits in maintenance activities and operations disruption avoidance have been observed, supporting business cases for system improvement.

Keywords: Aerospace, capability maturity model, software maturity, software lifecycle.

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Authors: Eduardo N. Velloso, Fernando M. N. Dantas, Luciano S. Barros

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A common complaint from power system analysis students lies in the overly complex tools they need to learn and use just to simulate very basic systems or just to check the answers to power system calculations. The most basic power system studies are power-flow solutions and short-circuit calculations. This paper presents a simple tool with an intuitive interface to perform both these studies and assess its performance in comparison with existent commercial solutions. With this in mind, Elegant is a pure Python software tool for learning power system analysis developed for undergraduate and graduate students. It solves the power-flow problem by iterative numerical methods and calculates bolted short-circuit fault currents by modeling the network in the domain of symmetrical components. Elegant can be used with a user-friendly Graphical User Interface (GUI) and automatically generates human-readable reports of the simulation results. The tool is exemplified using a typical Brazilian regional system with 18 buses. This study performs a comparative experiment with 1 undergraduate and 4 graduate students who attempted the same problem using both Elegant and a commercial tool. It was found that Elegant significantly reduces the time and labor involved in basic power system simulations while still providing some insights into real power system designs.

Keywords: Free- and open-source software, power-flow, power system analysis, Python, short-circuit.

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Authors: Hasan.O. Farahneh, Ayman A. Issa

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Software development is moving towards agility with use cases and scenarios being used for requirements stories. Estimates of software costs are becoming even more important than before as effects of delays is much larger in successive short releases context of agile development. Thus, this paper reports on the development of new linear use case based software cost estimation model applicable in the very early stages of software development being based on simple metric. Evaluation showed that accuracy of estimates varies between 43% and 55% of actual effort of historical test projects. These results outperformed those of wellknown models when applied in the same context. Further work is being carried out to improve the performance of the proposed model when considering the effect of non-functional requirements.

Keywords: Metrics, Software Cost Estimation, Use Cases

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Authors: M. Elhaj, M. Almrabet, M. Rgeai, I. Ehtiwesh

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A Comparison and evaluation of the different condition monitoring (CM) techniques was applied experimentally on RC e.g. Dynamic cylinder pressure and crankshaft Instantaneous Angular Speed (IAS), for the detection and diagnosis of valve faults in a two - stage reciprocating compressor for a programme of condition monitoring which can successfully detect and diagnose a fault in machine. Leakage in the valve plate was introduced experimentally into a two-stage reciprocating compressor. The effect of the faults on compressor performance was monitored and the differences with the normal, healthy performance noted as a fault signature been used for the detection and diagnosis of faults. The paper concludes with what is considered to be a unique approach to condition monitoring. First, each of the two most useful techniques is used to produce a Truth Table which details the circumstances in which each method can be used to detect and diagnose a fault. The two Truth Tables are then combined into a single Decision Table to provide a unique and reliable method of detection and diagnosis of each of the individual faults introduced into the compressor. This gives accurate diagnosis of compressor faults.

Keywords: Condition Monitoring, Dynamic Pressure, Instantaneous Angular Speed, Reciprocating Compressor.

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Authors: Renju Gangadharan, G. N. Pillai, Indra Gupta

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In this paper a novel method for finding the fault zone on a Thyristor Controlled Series Capacitor (TCSC) incorporated transmission line is presented. The method makes use of the Support Vector Machine (SVM), used in the classification mode to distinguish between the zones, before or after the TCSC. The use of Discrete Wavelet Transform is made to prepare the features which would be given as the input to the SVM. This method was tested on a 400 kV, 50 Hz, 300 Km transmission line and the results were highly accurate.

Keywords: Flexible ac transmission system (FACTS), thyristorcontrolled series-capacitor (TCSC), discrete wavelet transforms(DWT), support vector machine (SVM).

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Authors: Hina Gull, Farooque Azam, Wasi Haider Butt, Sardar Zafar Iqbal

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The software system goes through a number of stages during its life and a software process model gives a standard format for planning, organizing and running a project. The article presents a new software development process model named as “Divide and Conquer Process Model", based on the idea first it divides the things to make them simple and then gathered them to get the whole work done. The article begins with the backgrounds of different software process models and problems in these models. This is followed by a new divide and conquer process model, explanation of its different stages and at the end edge over other models is shown.

Keywords: Process Model, Waterfall, divide and conquer, Requirements.

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Authors: Salima Bella, Assia Belbachir, Ghalem Belalem

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This paper deals with the problem of monitoring and cleaning dirty zones of oceans using unmanned vehicles. We present a centralized cooperative architecture for unmanned aerial vehicles (UAVs) to monitor ocean regions and clean dirty zones with the help of unmanned surface vehicles (USVs). Due to the rapid deployment of these unmanned vehicles, it is convenient to use them in oceanic regions where the water pollution zones are generally unknown. In order to optimize this process, our solution aims to detect and reduce the pollution level of the ocean zones while taking into account the problem of fault tolerance related to these vehicles.

Keywords: Centralized architecture, fault tolerance, UAV, USV.

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Authors: Parvinder Singh Sandhu, Dalwinder Singh Salaria, Hardeep Singh

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Software Reusability is primary attribute of software quality. There are metrics for identifying the quality of reusable components but the function that makes use of these metrics to find reusability of software components is still not clear. These metrics if identified in the design phase or even in the coding phase can help us to reduce the rework by improving quality of reuse of the component and hence improve the productivity due to probabilistic increase in the reuse level. In this paper, we have devised the framework of metrics that uses McCabe-s Cyclometric Complexity Measure for Complexity measurement, Regularity Metric, Halstead Software Science Indicator for Volume indication, Reuse Frequency metric and Coupling Metric values of the software component as input attributes and calculated reusability of the software component. Here, comparative analysis of the fuzzy, Neuro-fuzzy and Fuzzy-GA approaches is performed to evaluate the reusability of software components and Fuzzy-GA results outperform the other used approaches. The developed reusability model has produced high precision results as expected by the human experts.

Keywords: Software Reusability, Software Metrics, Neural Networks, Genetic Algorithm, Fuzzy Logic.

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Authors: Nga Phuong Dang, Terje Vasskog, Ashwiny Pandey, Rajnish Kaur Calay

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Diatoms hold great potential for nutraceutical production as they are source of high value products such as eicosapentaenoic acid (EPA) and pigment fucoxanthin. EPA has proven human health benefits, and fucoxanthin can be used as both medicinal and nutritional ingredient to prevent and treat chronic diseases. The economic perspective of commercial production of a single product from microalgae is not attractive due to the high production cost. To improve the economic viability, we explore the concept of combining the production of both EPA and fucoxanthin in a single process. In our current study, we isolated twelve new microalgae isolates from Ofotfjord. Eight of them are diatoms and 4 of them are cyanobacteria and microalgae. Screening the new diatom isolates revealed that two strains are cold-tolerant diatom which can grow at 10 °C. They accumulated significant amount of lipid, which was up to 40-60% of the dried mass. The EPA contents from the two strains ranged from 15-19% of the total fatty acid, while fucoxanthin concentrations were between 1-1.4% of the dried biomass. Comparing with other studied diatom, this is promising result. The two strains hold promise as source for EPA and fucoxanthin production.

Keywords: Microalgae, fucoxanthin, eicosapentaenoic acid, diatom, fatty acid.

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Authors: Adel M. Sharaf, Foad H. Gandoman

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Nowadays, Photovoltaic-PV Farms/ Parks and large PV-Smart Grid Interface Schemes are emerging and commonly utilized in Renewable Energy distributed generation. However, PVhybrid- Dc-Ac Schemes using interface power electronic converters usually has negative impact on power quality and stabilization of modern electrical network under load excursions and network fault conditions in smart grid. Consequently, robust FACTS based interface schemes are required to ensure efficient energy utilization and stabilization of bus voltages as well as limiting switching/fault onrush current condition. FACTS devices are also used in smart grid- Battery Interface and Storage Schemes with PV-Battery Storage hybrid systems as an elegant alternative to renewable energy utilization with backup battery storage for electric utility energy and demand side management to provide needed energy and power capacity under heavy load conditions. The paper presents a robust interface PV-Li-Ion Battery Storage Interface Scheme for Distribution/Utilization Low Voltage Interface using FACTS stabilization enhancement and dynamic maximum PV power tracking controllers. Digital simulation and validation of the proposed scheme is done using MATLAB/Simulink software environment for Low Voltage- Distribution/Utilization system feeding a hybrid Linear-Motorized inrush and nonlinear type loads from a DC-AC Interface VSC-6- pulse Inverter Fed from the PV Park/Farm with a back-up Li-Ion Storage Battery.

Keywords: AC FACTS, Smart grid, Stabilization, PV-Battery Storage, Switched Filter-Compensation (SFC).

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Authors: N. Mansouri, A. Asadi

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Data Grid is a geographically distributed environment that deals with data intensive application in scientific and enterprise computing. Data replication is a common method used to achieve efficient and fault-tolerant data access in Grids. In this paper, a dynamic data replication strategy, called Enhanced Latest Access Largest Weight (ELALW) is proposed. This strategy is an enhanced version of Latest Access Largest Weight strategy. However, replication should be used wisely because the storage capacity of each Grid site is limited. Thus, it is important to design an effective strategy for the replication replacement task. ELALW replaces replicas based on the number of requests in future, the size of the replica, and the number of copies of the file. It also improves access latency by selecting the best replica when various sites hold replicas. The proposed replica selection selects the best replica location from among the many replicas based on response time that can be determined by considering the data transfer time, the storage access latency, the replica requests that waiting in the storage queue and the distance between nodes. Simulation results utilizing the OptorSim show our replication strategy achieve better performance overall than other strategies in terms of job execution time, effective network usage and storage resource usage.

Keywords: Data grid, data replication, simulation, replica selection, replica placement.

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Authors: Bushra Malik, Saad Zafar

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Inadequate curriculum for software engineering is considered to be one of the most common software risks. A number of solutions, on improving Software Engineering Education (SEE) have been reported in literature but there is a need to collectively present these solutions at one place. We have performed a mapping study to present a broad view of literature; published on improving the current state of SEE. Our aim is to give academicians, practitioners and researchers an international view of the current state of SEE. Our study has identified 70 primary studies that met our selection criteria, which we further classified and categorized in a well-defined Software Engineering educational framework. We found that the most researched category within the SE educational framework is Innovative Teaching Methods whereas the least amount of research was found in Student Learning and Assessment category. Our future work is to conduct a Systematic Literature Review on SEE.

Keywords: Mapping Study, Software Engineering, Software Engineering Education, Literature Survey.

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Authors: G. Ait Abdelmalek, R. Ziani

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In this document, we have proposed a robust conceptual strategy, in order to improve the robustness against the manufacturing defects and thus the reliability of logic CMOS circuits. However, in order to enable the use of future CMOS technology nodes this strategy combines various types of design: DFR (Design for Reliability), techniques of tolerance: hardware redundancy TMR (Triple Modular Redundancy) for hard error tolerance, the DFT (Design for Testability. The Results on largest ISCAS and ITC benchmark circuits show that our approach improves considerably the reliability, by reducing the key factors, the area costs and fault tolerance probability.

Keywords: Design for reliability, design for testability, fault tolerance, manufacturing yield.

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Authors: M. A. Hasan, M. R. Islam, R. A. Tarefder

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Currently, there are few user friendly Weigh-in- Motion (WIM) data analysis softwares available which can produce traffic input data for the recently developed AASHTOWare pavement Mechanistic-Empirical (ME) design software. However, these softwares have only rudimentary Quality Control (QC) processes. Therefore, they cannot properly deal with erroneous WIM data. As the pavement performance is highly sensible to the quality of WIM data, it is highly recommended to use more refined QC process on raw WIM data to get a good result. This study develops a userfriendly software, which can produce traffic input for the ME design software. This software takes the raw data (Class and Weight data) collected from the WIM station and processes it with a sophisticated QC procedure. Traffic data such as traffic volume, traffic distribution, axle load spectra, etc. can be obtained from this software; which can directly be used in the ME design software.

Keywords: Weigh-in-motion, software, axle load spectra, traffic distribution, AASHTOWare.

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Authors: Armin Rahimi

Abstract:

The Gravity Recovery and Climate Experiment (GRACE) has been a very successful project in determining math redistribution within the Earth system. Large deformations caused by earthquakes are in the high frequency band. Unfortunately, GRACE is only capable to provide reliable estimate at the low-to-medium frequency band for the gravitational changes. In this study, we computed the gravity changes after the 2012 Mw8.6 Indian Ocean earthquake off-Sumatra using the GRACE Level-2 monthly spherical harmonic (SH) solutions released by the University of Texas Center for Space Research (UTCSR). Moreover, we calculated gravity changes using different fault models derived from teleseismic data. The model predictions showed non-negligible discrepancies in gravity changes. However, after removing high-frequency signals, using Gaussian filtering 350 km commensurable GRACE spatial resolution, the discrepancies vanished, and the spatial patterns of total gravity changes predicted from all slip models became similar at the spatial resolution attainable by GRACE observations, and predicted-gravity changes were consistent with the GRACE-detected gravity changes. Nevertheless, the fault models, in which give different slip amplitudes, proportionally lead to different amplitude in the predicted gravity changes.

Keywords: Undersea earthquake, GRACE observation, gravity change, dislocation model, slip distribution.

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Authors: K. N. Dinesh Babu, P. K. Gargava

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Energization of a transformer results in sudden flow of current which is an effect of core magnetization. This current will be dominated by the presence of second harmonic, which in turn is used to segregate fault and inrush current, thus guaranteeing proper operation of the relay. This additional security in the relay sometimes obstructs or delays differential protection in a specific scenario, when the 2^nd harmonic content was present during a genuine fault. This kind of scenario can result in isolation of the transformer by Buchholz and pressure release valve (PRV) protection, which is acted when fault creates more damage in transformer. Such delays involve a huge impact on the insulation failure, and chances of repairing or rectifying fault of problem at site become very dismal. Sometimes this delay can cause fire in the transformer, and this situation becomes havoc for a sub-station. Such occurrences have been observed in field also when differential relay operation was delayed by 10-15 ms by second harmonic blocking in some specific conditions. These incidences have led to the need for an alternative solution to eradicate such unwarranted delay in operation in future. Modern numerical relay, called as intelligent electronic device (IED), is embedded with advanced protection features which permit higher flexibility and better provisions for tuning of protection logic and settings. Such flexibility in transformer protection IEDs, enables incorporation of alternative methods such as dynamic switching of second harmonic feature for blocking the differential protection with additional security. The analysis and precautionary measures carried out in this case, have been simulated and discussed in this paper to ensure that similar solutions can be adopted to inhibit analogous issues in future.

Keywords: Differential protection, intelligent electronic device (IED), 2nd harmonic, inrush inhibit.

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Authors: Halûk Gümüşkaya

Abstract:

In this paper we analyze the core issues affecting software architecture in enterprise projects where a large number of people at different backgrounds are involved and complex business, management and technical problems exist. We first give general features of typical enterprise projects and then present foundations of software architectures. The detailed analysis of core issues affecting software architecture in software development phases is given. We focus on three main areas in each development phase: people, process, and management related issues, structural (product) issues, and technology related issues. After we point out core issues and problems in these main areas, we give recommendations for designing good architecture. We observed these core issues and the importance of following the best software development practices and also developed some novel practices in many big enterprise commercial and military projects in about 10 years of experience.

Keywords: Software architecture, enterprise projects.

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Authors: Suma. V., T. R. Gopalakrishnan Nair

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Defect prevention is the most vital but habitually neglected facet of software quality assurance in any project. If functional at all stages of software development, it can condense the time, overheads and wherewithal entailed to engineer a high quality product. The key challenge of an IT industry is to engineer a software product with minimum post deployment defects. This effort is an analysis based on data obtained for five selected projects from leading software companies of varying software production competence. The main aim of this paper is to provide information on various methods and practices supporting defect detection and prevention leading to thriving software generation. The defect prevention technique unearths 99% of defects. Inspection is found to be an essential technique in generating ideal software generation in factories through enhanced methodologies of abetted and unaided inspection schedules. On an average 13 % to 15% of inspection and 25% - 30% of testing out of whole project effort time is required for 99% - 99.75% of defect elimination. A comparison of the end results for the five selected projects between the companies is also brought about throwing light on the possibility of a particular company to position itself with an appropriate complementary ratio of inspection testing.

Keywords: Defect Detection and Prevention, Inspections, Software Engineering, Software Process, Testing.

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Authors: Pham Thanh Trung, Huynh Quyet Thang

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Reliability is one of the most important quality attributes of software. Based on the approach of Reussner and the approach of Cheung, we proposed the reliability prediction model of component-based software architectures. Also, the value of the model is shown through the experimental evaluation on a web server system.

Keywords: component-based architecture, reliability prediction model, software reliability engineering.

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Authors: R. Abbasnia, F. Mohajeri Nav, S. Zahedifar, A. Tajik

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Recent years, adaptive pushover methods have been developed for seismic analysis of structures. Herein, the accuracy of the displacement-based adaptive pushover (DAP) method, which is introduced by Antoniou and Pinho [2004], is evaluated for Irregular buildings. The results are compared to the force-based procedure. Both concrete and steel frame structures, asymmetric in plan and elevation are analyzed and also torsional effects are taking into the account. These analyses are performed using both near fault and far fault records. In order to verify the results, the Incremental Dynamic Analysis (IDA) is performed.

Keywords: Pushover Analysis, DAP, IDA, Torsion.

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Authors: Úrsula B. Ferraz, Paulo F. Seixas, Webber E. Aguiar

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This paper presents a dynamic model for mechanical loads of an electric drive, including angular misalignment and including load unbalance. The misalignment model represents the effects of the universal joint between the motor and the mechanical load. Simulation results are presented for an induction motor driving a mechanical load with angular misalignment for both flexible and rigid coupling. The models presented are very useful in the study of mechanical fault detection in induction motors, using mechanical and electrical signals already available in a drive system, such as speed, torque and stator currents.

Keywords: Angular misalignment, fault modeling, unbalance, universal joint.

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Authors: Vijayalakshmy Periaswamy S., Kevin McDaid

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Testing accounts for the major percentage of technical contribution in the software development process. Typically, it consumes more than 50 percent of the total cost of developing a piece of software. The selection of software tests is a very important activity within this process to ensure the software reliability requirements are met. Generally tests are run to achieve maximum coverage of the software code and very little attention is given to the achieved reliability of the software. Using an existing methodology, this paper describes how to use Bayesian Belief Networks (BBNs) to select unit tests based on their contribution to the reliability of the module under consideration. In particular the work examines how the approach can enhance test-first development by assessing the quality of test suites resulting from this development methodology and providing insight into additional tests that can significantly reduce the achieved reliability. In this way the method can produce an optimal selection of inputs and the order in which the tests are executed to maximize the software reliability. To illustrate this approach, a belief network is constructed for a modern software system incorporating the expert opinion, expressed through probabilities of the relative quality of the elements of the software, and the potential effectiveness of the software tests. The steps involved in constructing the Bayesian Network are explained as is a method to allow for the test suite resulting from test-driven development.

Keywords: Software testing, Test Driven Development, Bayesian Belief Networks.

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Authors: Yasar Guneri Sahin, Mehmet Cudi Okur

Abstract:

Assessment of IEP (Individual Education Plan) is an important stage in the area of special education. This paper deals with this problem by introducing computer software which process the data gathered from application of IEP. The software is intended to be used by special education institution in Turkey and allows assessment of school and family trainings. The software has a user friendly interface and its design includes graphical developer tools.

Keywords: Disabled individual, software for education, assessment of education, special education.

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Authors: Hossein Mohammadpour, Rahman Dashti

Abstract:

Power transformers are among the most important and expensive equipments in the electric power systems. Consequently the transformer protection is an essential part of the system protection. This paper presents a new method for locating transformer winding faults such as turn-to-turn, turn-to-core, turn-totransformer body, turn-to-earth, and high voltage winding to low voltage winding. In this study the current and voltage signals of input and output terminals of the transformer are measured, which the Fourier transform of measured signals and harmonic analysis determine the fault's location.

Keywords: turn-to-turn faults, short circuit, Fourier transform, harmonic analysis.

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Authors: Avita K. Marihal, K.S. Jagadeesh, Sarita Sinha

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Pentachlorophenol (PCP) is a polychlorinated aromatic compound that is widespread in industrial effluents and is considered to be a serious pollutant. Among the variety of industrial effluents encountered, effluents from tanning industry are very important and have a serious pollution potential. PCP is also formed unintentionally in effluents of paper and pulp industries. It is highly persistent in soils and is lethal to a wide variety of beneficial microorganisms and insects, human beings and animals. The natural processes that breakdown toxic chemicals in the environment have become the focus of much attention to develop safe and environmentfriendly deactivation technologies. Microbes and plants are among the most important biological agents that remove and degrade waste materials to enable their recycling in the environment. The present investigation was carried out with the aim of developing a microbial system for bioremediation of PCP polluted soils. A number of plant species were evaluated for their ability to tolerate different concentrations of pentachlorophenol (PCP) in the soil. The experiment was conducted for 30 days under pot culture conditions. The toxic effect of PCP on plants was studied by monitoring seed germination, plant growth and biomass. As the concentration of PCP was increased to 50 ppm, the inhibition of seed germination, plant growth and biomass was also increased. Although PCP had a negative effect on all plant species tested, maize and groundnut showed the maximum tolerance to PCP. Other tolerating crops included wheat, safflower, sunflower, and soybean. From the rhizosphere soil of the tolerant seedlings, as many as twenty seven PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3; safflower 8; maize 2; groundnut and wheat, 3 each isolates were made. They were screened for their PCP degradation potentials. HPLC analyses of PCP degradation revealed that the isolate MAZ-2 degraded PCP completely. The isolate MAZ-1 was the next best isolate with 90 per cent PCP degradation. These strains hold promise to be used in the bioremediation of PCP polluted soils.

Keywords: Biodegradation, pentachlorophenol, rhizobacteria.

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Authors: M. M. Lâzâroaie

Abstract:

The high world interest given to the researches concerning the study of moderately halophilic solvent-tolerant bacteria isolated from marine polluted environments is due to their high biotechnological potential, and also to the perspective of their application in different remediation technologies. Using enrichment procedures, I isolated two moderately halophilic Gram-negative bacterial strains from seawater sample, which are tolerant to organic solvents. Cell tolerance, adhesion and cells viability of Aeromonas salmonicida IBBCt2 and Pseudomonas aeruginosa IBBCt3 in the presence of organic solvents depends not only on its physicochemical properties and its concentration, but also on the specific response of the cells, and the cellular response is not the same for these bacterial strains. n-hexane, n-heptane, propylbenzene, with log POW between 3.69 and 4.39, were less toxic for Aeromonas salmonicida IBBCt2 and Pseudomonas aeruginosa IBBCt3, compared with toluene, styrene, xylene isomers and ethylbenzene, with log POW between 2.64 and 3.17. The results indicated that Aeromonas salmonicida IBBCt2 is more susceptible to organic solvents than Pseudomonas aeruginosa IBBCt3. The mechanisms underlying solvent tolerance (e.g., the existance of the efflux pumps) in Aeromonas salmonicida IBBCt2 and Pseudomonas aeruginosa IBBCt3 it was also studied.

Keywords: bacteria, mechanisms, organic solvent, resistance.

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