Search results for: blood glucose measurement
1412 Optimization of Enzymatic Hydrolysis of Manihot Esculenta Root Starch by Immobilizeda-Amylase Using Response Surface Methodology
Authors: G. Baskar, C. Muthukumaran, S. Renganathan
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Enzymatic hydrolysis of starch from natural sources finds potential application in commercial production of alcoholic beverage and bioethanol. In this study the effect of starch concentration, temperature, time and enzyme concentration were studied and optimized for hydrolysis of cassava (Manihot esculenta) starch powder (of mesh 80/120) into glucose syrup by immobilized (using Polyacrylamide gel) a-amylase using central composite design. The experimental result on enzymatic hydrolysis of cassava starch was subjected to multiple linear regression analysis using MINITAB 14 software. Positive linear effect of starch concentration, enzyme concentration and time was observed on hydrolysis of cassava starch by a-amylase. The statistical significance of the model was validated by F-test for analysis of variance (p < 0.01). The optimum value of starch concentration temperature, time and enzyme concentration were found to be 4.5% (w/v), 45oC, 150 min, and 1% (w/v) enzyme. The maximum glucose yield at optimum condition was 5.17 mg/mL.Keywords: Enzymatic hydrolysis, Alcoholic beverage, Centralcomposite design, Polynomial model, glucose yield.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 22361411 Mathematical Modeling of the Working Principle of Gravity Gradient Instrument
Authors: Danni Cong, Meiping Wu, Hua Mu, Xiaofeng He, Junxiang Lian, Juliang Cao, Shaokun Cai, Hao Qin
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Gravity field is of great significance in geoscience, national economy and national security, and gravitational gradient measurement has been extensively studied due to its higher accuracy than gravity measurement. Gravity gradient sensor, being one of core devices of the gravity gradient instrument, plays a key role in measuring accuracy. Therefore, this paper starts from analyzing the working principle of the gravity gradient sensor by Newton’s law, and then considers the relative motion between inertial and non-inertial systems to build a relatively adequate mathematical model, laying a foundation for the measurement error calibration, measurement accuracy improvement.Keywords: Gravity gradient, accelerometer, gravity gradient sensor, single-axis rotation modulation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10631410 Statistical Optimization of Enzymatic Hydrolysis of Potato (Solanum tuberosum) Starch by Immobilized α-amylase
Authors: N.Peatciyammal, B.Balachandar, M.Dinesh Kumar, K.Tamilarasan, C.Muthukumaran
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Enzymatic hydrolysis of starch from natural sources finds potential application in commercial production of alcoholic beverage and bioethanol. In this study the effect of starch concentration, temperature, time and enzyme concentration were studied and optimized for hydrolysis of Potato starch powder (of mesh 80/120) into glucose syrup by immobilized (using Sodium arginate) α-amylase using central composite design. The experimental result on enzymatic hydrolysis of Potato starch was subjected to multiple linear regression analysis using MINITAB 14 software. Positive linear effect of starch concentration, enzyme concentration and time was observed on hydrolysis of Potato starch by α-amylase. The statistical significance of the model was validated by F-test for analysis of variance (p ≤ 0.01). The optimum value of starch concentration, enzyme concentration, temperature, time and were found to be 6% (w/v), 2% (w/v), 40°C and 80min respectively. The maximum glucose yield at optimum condition was 2.34 mg/mL.Keywords: Alcoholic beverage, Central Composite Design, Enzymatic hydrolysis, Glucose yield, Potato Starch.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 60371409 Test Method Development for Evaluation of Process and Design Effect on Reinforced Tube
Authors: Cathal Merz, Gareth O’Donnell
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Coil reinforced thin-walled (CRTW) tubes are used in medicine to treat problems affecting blood vessels within the body through minimally invasive procedures. The CRTW tube considered in this research makes up part of such a device and is inserted into the patient via their femoral or brachial arteries and manually navigated to the site in need of treatment. This procedure replaces the requirement to perform open surgery but is limited by reduction of blood vessel lumen diameter and increase in tortuosity of blood vessels deep in the brain. In order to maximize the capability of these procedures, CRTW tube devices are being manufactured with decreasing wall thicknesses in order to deliver treatment deeper into the body and to allow passage of other devices through its inner diameter. This introduces significant stresses to the device materials which have resulted in an observed increase in the breaking of the proximal segment of the device into two separate pieces after it has failed by buckling. As there is currently no international standard for measuring the mechanical properties of these CRTW tube devices, it is difficult to accurately analyze this problem. The aim of the current work is to address this discrepancy in the biomedical device industry by developing a measurement system that can be used to quantify the effect of process and design changes on CRTW tube performance, aiding in the development of better performing, next generation devices. Using materials testing frames, micro-computed tomography (micro-CT) imaging, experiment planning, analysis of variance (ANOVA), T-tests and regression analysis, test methods have been developed for assessing the impact of process and design changes on the device. The major findings of this study have been an insight into the suitability of buckle and three-point bend tests for the measurement of the effect of varying processing factors on the device’s performance, and guidelines for interpreting the output data from the test methods. The findings of this study are of significant interest with respect to verifying and validating key process and design changes associated with the device structure and material condition. Test method integrity evaluation is explored throughout.
Keywords: Buckling, coil reinforced thin-walled tubes, fracture, test method.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6961408 Warning about the Risk of Blood Flow Stagnation after Transcatheter Aortic Valve Implantation
Authors: Aymen Laadhari, Gábor Székely
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In this work, the hemodynamics in the sinuses of Valsalva after Transcatheter Aortic Valve Implantation is numerically examined. We focus on the physical results in the two-dimensional case. We use a finite element methodology based on a Lagrange multiplier technique that enables to couple the dynamics of blood flow and the leaflets’ movement. A massively parallel implementation of a monolithic and fully implicit solver allows more accuracy and significant computational savings. The elastic properties of the aortic valve are disregarded, and the numerical computations are performed under physiologically correct pressure loads. Computational results depict that blood flow may be subject to stagnation in the lower domain of the sinuses of Valsalva after Transcatheter Aortic Valve Implantation.
Keywords: Hemodynamics, Transcatheter Aortic Valve Implantation, blood flow stagnation, numerical simulations.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10971407 The Radial Pulse Wave and Blood Viscosity
Authors: Hyunhee Ryu, Young Ju Jeon, Jaeuk U. Kim, Hae Jung Lee, Yu Jung Lee, Jong Yeol Kim
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The aim of this study was to investigate the effect of blood viscosity on the radial pulse wave. For this, we obtained the radial pulse wave of 15 males with abnormal high hematocrit level and 47 males with normal hematocrit level at the age of thirties and forties. Various variables of the radial pulse wave between two groups were analyzed and compared by Student's T test. There are significant differences in several variables about height, time and area of the pulse wave. The first peak of the radial pulse wave was higher in abnormal high hematocrit group, but the third peak was higher and longer in normal hematocrit group. Our results suggest that the radial pulse wave can be used for diagnosis of high blood viscosity and more clinical application.
Keywords: Radial pulse wave, Blood viscosity, Hematocrit.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19681406 Towards an Intelligent Ontology Construction Cost Estimation System: Using BIM and New Rules of Measurement Techniques
Authors: F. H. Abanda, B. Kamsu-Foguem, J. H. M. Tah
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Construction cost estimation is one of the most important aspects of construction project design. For generations, the process of cost estimating has been manual, time-consuming and error-prone. This has partly led to most cost estimates to be unclear and riddled with inaccuracies that at times lead to over- or underestimation of construction cost. The development of standard set of measurement rules that are understandable by all those involved in a construction project, have not totally solved the challenges. Emerging Building Information Modelling (BIM) technologies can exploit standard measurement methods to automate cost estimation process and improve accuracies. This requires standard measurement methods to be structured in ontological and machine readable format; so that BIM software packages can easily read them. Most standard measurement methods are still text-based in textbooks and require manual editing into tables or Spreadsheet during cost estimation. The aim of this study is to explore the development of an ontology based on New Rules of Measurement (NRM) commonly used in the UK for cost estimation. The methodology adopted is Methontology, one of the most widely used ontology engineering methodologies. The challenges in this exploratory study are also reported and recommendations for future studies proposed.
Keywords: BIM, Construction projects, Cost estimation, NRM, Ontology.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 44421405 Evolution of Performance Measurement Methods in Conditions of Uncertainty: The Implementation of Fuzzy Sets in Performance Measurement
Authors: E. A. Tkachenko, E. M. Rogova, V. V. Klimov
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One of the basic issues of development management is connected with performance measurement as a prerequisite for identifying the achievement of development objectives. The aim of our research is to develop an improved model of assessing a company’s development results. The model should take into account the cyclical nature of development and the high degree of uncertainty in dealing with numerous management tasks. Our hypotheses may be formulated as follows: Hypothesis 1. The cycle of a company’s development may be studied from the standpoint of a project cycle. To do that, methods and tools of project analysis are to be used. Hypothesis 2. The problem of the uncertainty when justifying managerial decisions within the framework of a company’s development cycle can be solved through the use of the mathematical apparatus of fuzzy logic. The reasoned justification of the validity of the hypotheses made is given in the suggested article. The fuzzy logic toolkit applies to the case of technology shift within an enterprise. It is proven that some restrictions in performance measurement that are incurred to conventional methods could be eliminated by implementation of the fuzzy logic apparatus in performance measurement models.
Keywords: Fuzzy logic, fuzzy sets, performance measurement, project analysis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10781404 Dynamics of Blood Aminoacids in the Wounds- Treatment of Cows with Hydrocele Ointment
Authors: Marzhan Baimurzayeva, Alibek Utyanov, Gulnar Shabdarbaeva, Damir Khussainov
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This article introduces the actual problem that is а study of proposed by the authors Hydrocele ointment in amino acids’ metabolism of cows’ blood in inflammation of traumatic origin. Hydrocele ointment has shown a positive effect on inflammatory process and amino acids’ metabolism of animals treated with the drug. Amino acid levels reached physiological parameters on the 10th day after treatment; in the control group this parameter was higher than normal.Keywords: Amino acids, blood protein, Hydrocele ointment, inflammation, repair.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18241403 Optimization of Breast Tumor Cells Isolation Efficiency and Purity by Membrane Filtration
Authors: Bhuvanendran Nair Gourikutty Sajay, Liu Yuxin, Chang Chia-Pin, Poenar Daniel Puiu, Abdur Rub Abdur Rahman
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Size based filtration is one of the common methods employed to isolate circulating tumor cells (CTCs) from whole blood. It is well known that this method suffers from isolation efficiency to purity tradeoff. However, this tradeoff is poorly understood. In this paper, we present the design and manufacturing of a special rectangular slit filter. The filter was designed to retain maximal amounts of nucleated cells, while minimizing the pressure on cells, thereby preserving their morphology. The key parameter, namely, input pressure, was optimized to retain the maximal number of tumor cells, whilst maximizing the depletion of normal blood cells (red and white blood cells and platelets). Our results indicate that for a slit geometry of 5 × 40 μm on a 13 mm circular membrane with a fill factor of 21%, a pressure of 6.9 mBar yields the optimum for maximizing isolation of MCF-7 and depletion of normal blood cells.Keywords: Circulating tumor cells, Parylene slit membrane, Retention, White Blood Cell depletion.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18371402 Detection of Bias in GPS satellites- Measurements for Enhanced Measurement Integrity
Authors: Mamoun F. Abdel-Hafez
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In this paper, the detection of a fault in the Global Positioning System (GPS) measurement is addressed. The class of faults considered is a bias in the GPS pseudorange measurements. This bias is modeled as an unknown constant. The fault could be the result of a receiver fault or signal fault such as multipath error. A bias bank is constructed based on set of possible fault hypotheses. Initially, there is equal probability of occurrence for any of the biases in the bank. Subsequently, as the measurements are processed, the probability of occurrence for each of the biases is sequentially updated. The fault with a probability approaching unity will be declared as the current fault in the GPS measurement. The residual formed from the GPS and Inertial Measurement Unit (IMU) measurements is used to update the probability of each fault. Results will be presented to show the performance of the presented algorithm.
Keywords: Estimation and filtering, Statistical data analysis, Faultdetection and identification.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19651401 Lean Impact Analysis Assessment Models: Development of a Lean Measurement Structural Model
Authors: Catherine Maware, Olufemi Adetunji
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The paper is aimed at developing a model to measure the impact of Lean manufacturing deployment on organizational performance. The model will help industry practitioners to assess the impact of implementing Lean constructs on organizational performance. It will also harmonize the measurement models of Lean performance with the house of Lean that seems to have become the industry standard. The sheer number of measurement models for impact assessment of Lean implementation makes it difficult for new adopters to select an appropriate assessment model or deployment methodology. A literature review is conducted to classify the Lean performance model. Pareto analysis is used to select the Lean constructs for the development of the model. The model is further formalized through the use of Structural Equation Modeling (SEM) in defining the underlying latent structure of a Lean system. An impact assessment measurement model developed can be used to measure Lean performance and can be adopted by different industries.
Keywords: Impact measurement model, lean bundles, lean manufacturing, organizational performance.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12251400 Numerical Analysis and Experimental Validation of a Downhole Stress/Strain Measurement Tool
Authors: Abhay Bodake, Ping Sui, Hafeez Syed, Ratish Kadam
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Real-time measurement of applied forces, like tension, compression, torsion, and bending moment, identifies the transferred energies being applied to the bottomhole assembly (BHA). These forces are highly detrimental to measurement/logging-while-drilling tools and downhole equipment. Real-time measurement of the dynamic downhole behavior, including weight, torque, bending on bit, and vibration, establishes a real-time feedback loop between the downhole drilling system and drilling team at the surface. This paper describes the numerical analysis of the strain data acquired by the measurement tool at different locations on the strain pockets. The strain values obtained by FEA for various loading conditions (tension, compression, torque, and bending moment) are compared against experimental results obtained from an identical experimental setup. Numerical analyses results agree with experimental data within 8% and, therefore, substantiate and validate the FEA model. This FEA model can be used to analyze the combined loading conditions that reflect the actual drilling environment.
Keywords: FEA, M/LWD, Oil & Gas, Strain Measurement.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 25851399 Simulation and Measurement the Radiation of an Antenna inside a Metallic Case using FDTD
Authors: Shabnam Ladan, M. S. Abrishamian
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In this paper we have developed a FDTD simulation code which can treat wave propagation of a monopole antenna in a metallic case which covers with PML, and performed a series of three dimensional FDTD simulations of electromagnetic wave propagation in this space .We also provide a measurement set up in antenna lab and fortunately the simulations and measurements show good agreement. According to simulation and measurement results, we confirmed that the computer program which had been written in FORTRAN, works correctly.Keywords: FDTD, EMC, monopole antenna.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15341398 Performance Complexity Measurement of Tightening Equipment Based on Kolmogorov Entropy
Authors: Guoliang Fan, Aiping Li, Xuemei Liu, Liyun Xu
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The performance of the tightening equipment will decline with the working process in manufacturing system. The main manifestations are the randomness and discretization degree increasing of the tightening performance. To evaluate the degradation tendency of the tightening performance accurately, a complexity measurement approach based on Kolmogorov entropy is presented. At first, the states of performance index are divided for calibrating the discrete degree. Then the complexity measurement model based on Kolmogorov entropy is built. The model describes the performance degradation tendency of tightening equipment quantitatively. At last, a study case is applied for verifying the efficiency and validity of the approach. The research achievement shows that the presented complexity measurement can effectively evaluate the degradation tendency of the tightening equipment. It can provide theoretical basis for preventive maintenance and life prediction of equipment.
Keywords: Complexity measurement, Kolmogorov entropy, manufacturing system, performance evaluation, tightening equipment.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 9611397 A Method for Measurement and Evaluation of Drape of Textiles
Authors: L. Fridrichova, R. Knížek, V. Bajzík
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Drape is one of the important visual characteristics of the fabric. This paper is introducing an innovative method of measurement and evaluation of the drape shape of the fabric. The measuring principle is based on the possibility of multiple vertical strain of the fabric. This method more accurately simulates the real behavior of the fabric in the process of draping. The method is fully automated, so the sample can be measured by using any number of cycles in any time horizon. Using the present method of measurement, we are able to describe the viscoelastic behavior of the fabric.
Keywords: Drape, drape shape, automated drape meter.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 8771396 Conducting Flow Measurement Laboratory Test Work
Authors: M. B. Kime
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Mass flow measurement is the basis of most technoeconomic formulations in the chemical industry. This calls for reliable and accurate detection of mass flow. Flow measurement laboratory experiments were conducted using various instruments. These consisted of orifice plates, various sized rotameters, wet gas meter and soap bubble meter. This work was aimed at evaluating appropriate operating conditions and accuracy of the aforementioned devices. The experimental data collected were compared to theoretical predictions from Bernoulli’s equation and calibration curves supplied by the instrument’s manufacturers. The results obtained showed that rotameters were more reliable for measuring high and low flow rates; while soap-bubble meters and wet-gas meters were found to be suitable for measuring low flow rates. The laboratory procedures and findings of the actual work can assist engineering students and professionals in conducting their flow measurement laboratory test work.
Keywords: Flow measurement, orifice plates, rotameters, wet gas meter, soap bubble meter.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 49421395 Relationship between Behavioral Inhibition/Approach System and Perceived Stress: With White Blood Cell in Multiple Sclerosis Patients
Authors: Amin Alvani
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Multiple sclerosis (MS) is a chronic, often disabling disease in which the immune system attacks the myelin sheath of neurons in the central nervous system. The purpose of this study was to explore the correlation between the Behavioral Inhibition/Approach System (BIS-BAS) and Perceived Stress (PS), while controlling for White Blood Cell (WBC) count. 60 MS patients (36.7% male, 63.3% female; aged 15-65 years) participated in this study. They completed a demographic questionnaire, underwent a complete blood cell (CBC) test, filled out the Behavioral Activation and Behavioral Inhibition Scale (BIS-BAS), and responded to the Perceived Stress Questionnaire (PSS-14). The results indicated a significant relationship between the BAS-Reward Responsiveness (BAS-RR) subscale and PS, particularly in a subset of MS patients with increased WBC counts.
Keywords: Behavioral inhibition/approach system, multiple sclerosis, perceived stress, white blood cell.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 571394 Radio Technology Frequency Identification Applied in High-Voltage Power Transmission- Line for Sag Measurement
Authors: Tlotlollo Sidwell Hlalele, Shengzhi Du
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High-voltage power transmission lines are the back bone of electrical power utilities. The stability and continuous monitoring of this critical infrastructure is pivotal. Nine-Sigma representing Eskom Holding SOC limited, South Africa has a major problem on proactive detection of fallen power lines and real time sagging measurement together with slipping of such conductors. The main objective of this research is to innovate RFID technology to solve this challenge. Various options and technologies such as GPS, PLC, image processing, MR sensors and etc., have been reviewed and draw backs were made. The potential of RFID to give precision measurement will be observed and presented. The future research will look at magnetic and electrical interference as well as corona effect on the technology.
Keywords: Precision Measurement, RFID and Sag.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 24261393 Genetic Variants and Atherosclerosis
Authors: M. Seifi, A. Ghasemi, M. Khosravi, M. Salimi, S. Jahandideh, J. Sherizadeh, F. S. Hashemizadeh, R. Khodaei
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Atherosclerosis is the condition in which an artery wall thickens as the result of a build-up of fatty materials such as cholesterol. It is a syndrome affecting arterial blood vessels, a chronic inflammatory response in the walls of arteries, in large part due to the accumulation of macrophage white blood cells and promoted by low density (especially small particle) lipoproteins (plasma proteins that carry cholesterol and triglycerides) without adequate removal of fats and cholesterol from the macrophages by functional high density lipoproteins (HDL). It is commonly referred to as a hardening or furring of the arteries. It is caused by the formation of multiple plaques within the arteries.Keywords: Arterial blood vessels, atherosclerosis, cholesterol.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15621392 A Development of the Multiple Intelligences Measurement of Elementary Students
Authors: Chaiwat Waree
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This research aims at development of the Multiple Intelligences Measurement of Elementary Students. The structural accuracy test and normality establishment are based on the Multiple Intelligences Theory of Gardner. This theory consists of eight aspects namely linguistics, logic and mathematics, visual-spatial relations, body and movement, music, human relations, self-realization/selfunderstanding and nature. The sample used in this research consists of elementary school students (aged between 5-11 years). The size of the sample group was determined by Yamane Table. The group has 2,504 students. Multistage Sampling was used. Basic statistical analysis and construct validity testing were done using confirmatory factor analysis. The research can be summarized as follows; 1. Multiple Intelligences Measurement consisting of 120 items is content-accurate. Internal consistent reliability according to the method of Kuder-Richardson of the whole Multiple Intelligences Measurement equals .91. The difficulty of the measurement test is between .39-.83. Discrimination is between .21-.85. 2). The Multiple Intelligences Measurement has construct validity in a good range, that is 8 components and all 120 test items have statistical significance level at .01. Chi-square value equals 4357.7; p=.00 at the degree of freedom of 244 and Goodness of Fit Index equals 1.00. Adjusted Goodness of Fit Index equals .92. Comparative Fit Index (CFI) equals .68. Root Mean Squared Residual (RMR) equals 0.064 and Root Mean Square Error of Approximation equals 0.82. 3). The normality of the Multiple Intelligences Measurement is categorized into 3 levels. Those with high intelligence are those with percentiles of more than 78. Those with moderate/medium intelligence are those with percentiles between 24 and 77.9. Those with low intelligence are those with percentiles from 23.9 downwards.
Keywords: Multiple Intelligences, Measurement, Elementary Students.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 29581391 Resistance Training as a Powerful Tool in the Prevention and Treatment of Cardiovascular Diseases
Authors: I. Struhár, L. Dovrtělová, M. Kumstát
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Regular exercise promotes reduction in blood pressure, reduction in body weight and it also helps to increase in insulin sensitivity. Participation in physical activity should always be linked to medical screening which can reveal serious medical problems. One of them is high blood pressure. Hypertension is risk factor for one billion people worldwide and the highest prevalence is found in Africa. Another component of hypertension is that people who suffer from hypertension have no symptoms. It is estimated that reduction of 3mm Hg in Systolic Blood Pressure decreases cardiac morbidity at least 5%. The most of the guidelines suggest aerobic exercise in a prevention of cardiovascular diseases. On the other hand, it is important to emphasize the impact of resistance training. Even, it was found higher effect for reduction on the level of systolic blood pressure than aerobic exercise.
Keywords: Coronary artery disease, physical activity, prevention, resistance training.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19711390 Effect of Oxytocin on Cytosolic Calcium Concentration of Alpha and Beta Cells in Pancreas
Authors: Rauza Sukma Rita, Katsuya Dezaki, Yuko Maejima, Toshihiko Yada
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Oxytocin is a nine-amino acid peptide synthesized in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the hypothalamus. Oxytocin promotes contraction of the uterus during birth and milk ejection during breast feeding. Although oxytocin receptors are found predominantly in the breasts and uterus of females, many tissues and organs express oxytocin receptors, including the pituitary, heart, kidney, thymus, vascular endothelium, adipocytes, osteoblasts, adrenal gland, pancreatic islets, and many cell lines. On the other hand, in pancreatic islets, oxytocin receptors are expressed in both α-cells and β-cells with stronger expression in α- cells. However, to our knowledge there are no reports yet about the effect of oxytocin on cytosolic calcium reaction on α and β-cell. This study aims to investigate the effect of oxytocin on α-cells and β-cells and its oscillation pattern. Islet of Langerhans from wild type mice were isolated by collagenase digestion. Isolated and dissociated single cells either α-cells or β-cells on coverslips were mounted in an open chamber and superfused in HKRB. Cytosolic concentration ([Ca2+]i) in single cells were measured by fura-2 microfluorimetry. After measurement of [Ca2+]i, α-cells were identified by subsequent immunocytochemical staining using an anti-glucagon antiserum. In β-cells, the [Ca2+]i increase in response to oxytocin was observed only under 8.3 mM glucose condition, whereas in α-cells, [Ca2+]i an increase induced by oxytocin was observed in both 2.8 mM and 8.3 mM glucose. The oscillation incidence was induced more frequently in β-cells compared to α-cells. In conclusion, the present study demonstrated that oxytocin directly interacts with both α-cells and β-cells and induces increase of [Ca2+]i and its specific patterns.
Keywords: α-cells, β-cells, cytosolic calcium concentration, oscillation, oxytocin.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10901389 Object Localization in Medical Images Using Genetic Algorithms
Authors: George Karkavitsas, Maria Rangoussi
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We present a genetic algorithm application to the problem of object registration (i.e., object detection, localization and recognition) in a class of medical images containing various types of blood cells. The genetic algorithm approach taken here is seen to be most appropriate for this type of image, due to the characteristics of the objects. Successful cell registration results on real life microscope images of blood cells show the potential of the proposed approach.
Keywords: Genetic algorithms, object registration, pattern recognition, blood cell microscope images.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19681388 Study of Effect Different of Ozone Doses on Sugars Content in Tomatoes at Different Stages of Ripening
Authors: Milad. A. Shalluf
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The determination of sugars in foods is very significant. Their relation in fact, can affect the chemical and sensorial quality of the matrix (e.g., sweetness, pH, total acidity, microbial stability, global acceptability) and can provide information on food to optimize several selected technological processes. Three stages of ripeness (green, yellow and red) of tomatoes (Lycopersicon Esculentum cv. Elegance) at different harvest dates were evaluated. Fruit from all harvests were exposed to different of ozone doses (0.25, 0.50 and 1 mg O3/g tomatoes) and clean air for 5 day at 15 °C±2 and 90-95 % relative humidity. Then, fruits were submitted for extraction and analysis after a day from the finish of exposure of each stage. The concentrations of the glucose and fructose increased in the tomatoes which were subjected to ozone treatments.Keywords: Post-harvest Treatment, Controlled Atmosphere Storage, Ozone, Tomatoes, Glucose, Fructose
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19301387 Ultra-Precise Hybrid Lens Distortion Correction
Authors: Christian Bräuer-Burchardt, Peter Kühmstedt, Gunther Notni
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A new hybrid method to realise high-precision distortion determination for optical ultra-precision 3D measurement systems based on stereo cameras using active light projection is introduced. It consists of two phases: the basic distortion determination and the refinement. The refinement phase of the procedure uses a plane surface and projected fringe patterns as calibration tools to determine simultaneously the distortion of both cameras within an iterative procedure. The new technique may be performed in the state of the device “ready for measurement" which avoids errors by a later adjustment. A considerable reduction of distortion errors is achieved and leads to considerable improvements of the accuracy of 3D measurements, especially in the precise measurement of smooth surfaces.Keywords: 3D Surface Measurement, Fringe Projection, Lens Distortion, Stereo.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20671386 Leukocyte Detection Using Image Stitching and Color Overlapping Windows
Authors: Lina, Arlends Chris, Bagus Mulyawan, Agus B. Dharmawan
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Blood cell analysis plays a significant role in the diagnosis of human health. As an alternative to the traditional technique conducted by laboratory technicians, this paper presents an automatic white blood cell (leukocyte) detection system using Image Stitching and Color Overlapping Windows. The advantage of this method is to present a detection technique of white blood cells that are robust to imperfect shapes of blood cells with various image qualities. The input for this application is images from a microscope-slide translation video. The preprocessing stage is performed by stitching the input images. First, the overlapping parts of the images are determined, then stitching and blending processes of two input images are performed. Next, the Color Overlapping Windows is performed for white blood cell detection which consists of color filtering, window candidate checking, window marking, finds window overlaps, and window cropping processes. Experimental results show that this method could achieve an average of 82.12% detection accuracy of the leukocyte images.Keywords: Color overlapping windows, image stitching, leukocyte detection.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14921385 The Study of Super Hydrophobic Surfaces Using High Speed Shadowgraphy
Authors: D. Jasikova, M. Kotek, V. Kopecky
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The aim of this article is the measurement of the basic characteristic of superhydrophobic surfaces using high speed shadowgraphy. Here we describe the novel patented system for the industrial production of superhydrophobic surfaces. These surfaces were investigated with two optically based measurement methods: impinging drop and inclined wall. The results of the visualization and analysis help to state the suitable sample with superhydrophobic properties for mathematic simulation.
Keywords: Antipearl effect, contact angle, hydrophobic, impinging drop, inclined wall, measurement, plasma, shadowgraphy, superhydrophobic surface.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 24581384 Anaplasmosis among Camels in Iran and Observation of Abnormalities in Infected Blood Films
Authors: Khosro Ghazvinian, Touba Khodaiean
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Anaplasma organisms are obligatory intracellular bacteria belonging to the order Rickettsiales, family Anaplasmataceae. This disease is distributed around the globe and infected ticks are the most important vectors in anaplasmosis transmission. There is a little information about anaplasmosis in camels. This research investigated the blood films of 35 (20 male, 15 female) camels randomly selected from a flock of 150 camels. Samples were stained with Giemsa and Anaplasma sp. organisms were observed in six out of 35 (17.14 %) blood films. There were also some changes in Diff-Quick and morphology of leukocytes. No significant difference between male and female camels was observed (P>0.05). According to the results anaplasmosis is presented among camels in Iran.
Keywords: Anaplasma, camel, anaplasmosis, Iran.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10381383 Developing Measurement Model of Interpersonal Skills of Youth
Authors: Mohd Yusri Ibrahim
Abstract:
Although it is known that interpersonal skills are essential for personal development, the debate however continues as to how to measure those skills, especially in youths. This study was conducted to develop a measurement model of interpersonal skills by suggesting three construct namely personal, skills and relationship; six function namely self, perception, listening, conversation, emotion and conflict management; and 30 behaviours as indicators. This cross-sectional survey by questionnaires was applied in east side of peninsula of Malaysia for 150 respondents, and analyzed by structural equation modelling (SEM) by AMOS. The suggested constructs, functions and indicators were consider accepted as measurement elements by observing on regression weight for standard loading, average variance extracted (AVE) for convergent validity, square root of AVE for discriminant validity, composite reliability (CR), and at least three fit indexes for model fitness. Finally, a measurement model of interpersonal skill for youth was successfully developed.
Keywords: Interpersonal communication, interpersonal skill, youth.
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