%0 Journal Article
	%A Bhuvanendran Nair Gourikutty Sajay and  Liu Yuxin and  Chang Chia-Pin and  Poenar Daniel Puiu and  Abdur Rub
Abdur Rahman
	%D 2012
	%J International Journal of Biomedical and Biological Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 69, 2012
	%T Optimization of Breast Tumor Cells Isolation Efficiency and Purity by Membrane Filtration
	%U https://publications.waset.org/pdf/9841
	%V 69
	%X Size based filtration is one of the common methods
employed to isolate circulating tumor cells (CTCs) from whole
blood. It is well known that this method suffers from isolation
efficiency to purity tradeoff. However, this tradeoff is poorly
understood. In this paper, we present the design and manufacturing
of a special rectangular slit filter. The filter was designed to retain
maximal amounts of nucleated cells, while minimizing the pressure
on cells, thereby preserving their morphology. The key parameter,
namely, input pressure, was optimized to retain the maximal number
of tumor cells, whilst maximizing the depletion of normal blood cells
(red and white blood cells and platelets). Our results indicate that for
a slit geometry of 5 × 40 μm on a 13 mm circular membrane with a
fill factor of 21%, a pressure of 6.9 mBar yields the optimum for
maximizing isolation of MCF-7 and depletion of normal blood cells.
	%P 451 - 454