Search results for: temperature separation
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 7851

Search results for: temperature separation

7641 CFD Simulation of the Inlet Pressure Effects on the Cooling Capacity Enhancement for Vortex Tube with Couple Vortex Chambers

Authors: Nader Pourmahmoud, Amir Hassanzadeh

Abstract:

This article investigates the effects of inlet pressure in a newly introduced vortex tube which has been equipped with an additional vortex chamber. A 3-D compressible turbulent flow computation has been carried out toward analysis of complex flow field in this apparatus. Numerical results of flows are derived by utilizing the standard k-ε turbulence model for analyzing high rotating complex flow field. The present research has focused on cooling effect and given a characteristics curve for minimum cool temperature. In addition, the effect of inlet pressure for both chambers has been studied in details. To be presented numerical results show that the effect of inlet pressure in second chamber has more important role in improving the performance of the vortex tube than first one. By increasing the pressure in the second chamber, cold outlet temperature reaches a higher decrease. When both chambers are fed with high pressure fluid, best operation condition of vortex tube occurs. However, it is not possible to feed both chambers with high pressure due to the conditions of working environment.

Keywords: energy separation, inlet pressure, numerical simulation, vortex chamber, vortex tube

Procedia PDF Downloads 344
7640 Modelling for Temperature Non-Isothermal Continuous Stirred Tank Reactor Using Fuzzy Logic

Authors: Nasser Mohamed Ramli, Mohamad Syafiq Mohamad

Abstract:

Many types of controllers were applied on the continuous stirred tank reactor (CSTR) unit to control the temperature. In this research paper, Proportional-Integral-Derivative (PID) controller are compared with Fuzzy Logic controller for temperature control of CSTR. The control system for temperature non-isothermal of a CSTR will produce a stable response curve to its set point temperature. A mathematical model of a CSTR using the most general operating condition was developed through a set of differential equations into S-function using MATLAB. The reactor model and S-function are developed using m.file. After developing the S-function of CSTR model, User-Defined functions are used to link to SIMULINK file. Results that are obtained from simulation and temperature control were better when using Fuzzy logic control compared to PID control.

Keywords: CSTR, temperature, PID, fuzzy logic

Procedia PDF Downloads 419
7639 Synthesis of Visible-Light-Driven Magnetically Recoverable N-TiO2@SiO2@Fe3O4 Nanophotocatalyst for Enhanced Degradation of Ibuprofen

Authors: Ashutosh Kumar, Irene M. C. Lo

Abstract:

Ever since the discovery of TiO2 for decomposition of cyanide in water, it has been investigated extensively for the photocatalytic degradation of environmental pollutants, and became the most practical and prevalent photocatalyst. The superiority of TiO2 is due to its chemical and biological inertness, nontoxicity, strong oxidizing power and cost-effectiveness. However, during degradation of pollutants in wastewater, it suffers from problems, such as (a) separation after use, and (b) its poor photocatalytic performance under visible light irradiation (~45% of the solar spectrum). In order to bridge the research gaps, N-TiO2@SiO2@Fe3O4 nanophotocatalysts of average size 19 nm and effective surface area 47 m2 gm-1 were synthesized using sol-gel method. The characterization was performed using BET, TEM-EDX, VSM and XRD. The performance was improved by considering different factors involved during the synthesis, such as calcination temperature, amount of Fe3O4 nanoparticles used and amount of urea used for N-doping. The final nanophotocatalyst was calcined at 500 °C which was able to degrade 94% of the ibuprofen within 5 h of irradiation time. Under the influence of ~200 mT electromagnetic field, 95% nanophotocatalysts separation efficiency was achieved within 20-25 min. Moreover, the effect of different visible light source of similar irradiance, such as compact fluorescent lamp (CFL) and light emitting diode (LED), is also investigated in this research. The performance of nanophotocatalysts was found to be comparatively higher under ~310 µW cm-2 irradiance with peak emissive wavelengths of 543 nm emitted by CFL. Therefore, a promising visible-light-driven magnetically separable TiO2-based nanophotocatalysts was synthesized for the efficient degradation of ibuprofen.

Keywords: ibuprofen, magnetic N-TiO2, photocatalysis, visible light sources

Procedia PDF Downloads 219
7638 Influence of Pouring Temperature on the Formation of Spheroidal and Lamellar Graphite in Cast Iron

Authors: Mehmet Ekici

Abstract:

The objective of this research is to investigate the effect of pouring temperature on the microstructure of the cast iron. The pattern was designed with 300 mm of width, and the thickness variations are 1.25 mm and poured at five different temperatures; 1300, 1325, 1350, 1375 and 1400°C. Several cast irons, prepared with different chemical compositions and microstructures (three lamellar and three spheroidal structures) have been examined by extensive mechanical testing and optical microscopy. The fluidity of spheroidal and lamellar graphite in cast iron increases with the pouring temperature. The numbers of nodules were decreased by increasing pouring temperature for spheroidal structures. Whereas, the numbers of flakes of lamellar structures changed by both pouring temperature and chemical composition. In general, with increasing pouring temperature, the amount of pearlite in the internal structure of both lamellar and spheroidal graphite cast iron materials were increased.

Keywords: spheroidal graphite cast iron, lamellar graphite in cast iron, pouring temperature, tensile test and impact test

Procedia PDF Downloads 305
7637 Evaluation of Thermal Barrier Coating According to Temperature and Curvature

Authors: Hyunwoo Song, Jeong-Min Lee, Yongseok Kim, Junghan Yun, Jungin Byun, Jae-Mean Koo, Chang-Sung Seok

Abstract:

To avoid the damage of gas turbine blade from high-temperature, thermal barrier coating (TBC) is applied on the blade. However, it is damaged by thermal fatigue during the operation of gas turbine, and this damage lead to delamination of TBC between top coat and bond coat. The blade can be damaged after the failure of TBC, so durability evaluation of TBC should be performed. The durability of thermal barrier coating was decreased according to the increase of temperature, because thermal stress according to increase of temperature. Also, the curvature can be affect to durability of TBC, because the stress is determined by the shape of the TBC. Therefore, the effect of temperature and curvature on the stress should be evaluated. In this study, finite element analysis according to temperature and curvature were performed in the same condition of Kim et al. Finally, the stress was evaluated from the finite element analysis results according to temperature and curvature.

Keywords: curvature, finite element analysis, thermal barrier coating, thermal fatigue, temperature

Procedia PDF Downloads 527
7636 Temperature Effect on Sound Propagation in an Elastic Pipe with Viscoelastic Liquid

Authors: S. Levitsky, R. Bergman

Abstract:

Fluid rheology may have essential impact on sound propagation in a liquid-filled pipe, especially, in a low frequency range. Rheological parameters of liquid are temperature-sensitive, which ultimately results in a temperature dependence of the wave speed and attenuation in the waveguide. The study is devoted to modeling of this effect at sound propagation in an elastic pipe with polymeric liquid, described by generalized Maxwell model with non-zero high-frequency viscosity. It is assumed that relaxation spectrum is distributed according to the Spriggs law; temperature impact on the liquid rheology is described on the basis of the temperature-superposition principle and activation theory. The dispersion equation for the waveguide, considered as a thin-walled tube with polymeric solution, is obtained within a quasi-one-dimensional formulation. Results of the study illustrate the influence of temperature on sound propagation in the system.

Keywords: elastic tube, sound propagation, temperature effect, viscoelastic liquid

Procedia PDF Downloads 387
7635 Transport and Mixing Phenomena Developed by Vortex Formation in Flow around Airfoil Using Lagrangian Coherent Structures

Authors: Riaz Ahmad, Jiazhong Zhang, Asma Farooqi

Abstract:

In this study, mass transport between separation bubbles and the flow around a two-dimensional airfoil are numerically investigated using Lagrangian Coherent Structures (LCSs). Finite Time Lyapunov Exponent (FTLE) technique is used for the computation to identify invariant manifolds and LCSs. Moreover, the Characteristic Base Split (CBS) scheme combined with dual time stepping technique is applied to simulate such transient flow at low Reynolds number. We then investigate the evolution of vortex structures during the transport process with the aid of LCSs. To explore the vortex formation at the surface of the airfoil, the dynamics of separatrix is also taken into account which is formed by the combination of stable-unstable manifolds. The Lagrangian analysis gives a detailed understanding of vortex dynamics and separation bubbles which plays a significant role to explore the performance of the unsteady flow generated by the airfoil. Transport process and flow separation phenomena are studied extensively to analyze the flow pattern by Lagrangian point of view.

Keywords: transport phenomena, CBS Method, vortex formation, Lagrangian Coherent Structures

Procedia PDF Downloads 108
7634 Temperature Distribution Simulation of Divergent Fluid Flow with Helical Arrangement

Authors: Ehan Sabah Shukri, Wirachman Wisnoe

Abstract:

Numerical study is performed to investigate the temperature distribution in an annular diffuser fitted with helical tape hub. Different pitches (Y = 20 mm, and Y = 30 mm) for the helical tape are studied with different heights (H = 20 mm, 22 mm, and 24 mm) to be compared. The geometry of the annular diffuser and the inlet condition for both hub arrangements are kept constant. The result obtains that using helical tape insert with different pitches and different heights will force the temperature to distribute in a helical direction; however the use of helical tape hub with height (H = 22 mm) for both pitches enhance the temperature distribution in a good manner.

Keywords: helical tape, divergent fluid flow, temperature distribution, swirl flow, CFD

Procedia PDF Downloads 422
7633 Numerical Study of Off-Design Performance of a Highly Loaded Low Pressure Turbine Cascade

Authors: Shidvash Vakilipour, Mehdi Habibnia, Rouzbeh Riazi, Masoud Mohammadi, Mohammad H. Sabour

Abstract:

The flow field passing through a highly loaded low pressure (LP) turbine cascade is numerically investigated at design and off-design conditions. The Field Operation And Manipulation (OpenFOAM) platform is used as the computational Fluid Dynamics (CFD) tool. Firstly, the influences of grid resolution on the results of k-ε, k-ω, and LES turbulence models are investigated and compared with those of experimental measurements. A numerical pressure under-shoot is appeared near the end of blade pressure surface which is sensitive to grid resolution and flow turbulence modeling. The LES model is able to resolve separation on a coarse and fine grid resolutions. Secondly, the off-design flow condition is modeled by negative and positive inflow incidence angles. The numerical experiments show that a separation bubble generated on blade pressure side is predicted by LES. The total pressure drop is also been calculated at incidence angle between -20◦ and +8◦. The minimum total pressure drop is obtained by k-ω and LES at the design point.

Keywords: low pressure turbine, off-design performance, openFOAM, turbulence modeling, flow separation

Procedia PDF Downloads 331
7632 Power and Efficiency of Photovoltaic Module: Effect of Cell Temperature

Authors: R. Nasrin, M. Ferdows

Abstract:

Among the renewable energy sources, photovoltaic (PV) is a high potential, effective, and sustainable system. Irradiation intensity from 200 W/m2 to 1000 W/m2 has been considered to observe the performance of PV module. Generally, this module converts only about 15% - 20% of incident irradiation into electrical energy and the rest part is converted into heat energy. Finite element method has been used to solve the problem numerically. Simulation has been performed by considering the ambient temperature 30°C. Higher irradiation increase solar cell temperature and electrical power. The electrical efficiency of PV module decreases with the variation of solar radiation. The efficiency of PV module can be increased if cell temperature is reduced. Thus the effect of irradiation is significant to enhance the efficiency of PV module if the solar cell temperature is kept at a certain level.

Keywords: PV module, solar radiation, efficiency, cell temperature

Procedia PDF Downloads 334
7631 A Study on the Magnetic and Mechanical Properties of Nd-Fe-B Sintered Magnets According to Sintering Temperature

Authors: J. H. Kim, S. Y. Park, K. M. Lim, S. K. Hyun

Abstract:

The effect of sintering temperature on the magnetic and mechanical properties of Nd-Fe-B sintered magnets has been investigated in this study. The sintering temperature changed from 950°C to 1120°C. While remanence and hardness of the magnets increased with increasing sintering temperature, the coercivity first increased, and then decreased. The optimum magnetic and mechanical properties of the magnets were obtained at the sintering temperature of 1050°C. In order to clarify the reason for the variation on magnetic and mechanical properties of the magnets, we systematically analyzed the microstructure.

Keywords: magnetic and mechanical property, microstructure, permanent magnets, sintered Nd-Fe-B magnet

Procedia PDF Downloads 302
7630 Pre-Service Teachers’ Conceptual Representations of Heat and Temperature

Authors: Abdeljalil Métioui

Abstract:

The purpose of this paper is to present the results of research on the conceptual representations of 128 Quebec (Canada) pre-service teachers enrolled in their third year of university in a program to train elementary teachers about heat and temperature. To identify their conceptual representations about heat and temperature, we constructed a multiple-choice questionnaire consisting of five questions. For each question, they had to explain their choice of an answer. At the methodological level, this step is essential to be able to identify the student conceptual representations. It should be noted that the selected questions were based: (1) on the works have done worldwide on primary and secondary students’ misconceptions about heat and temperature; (2) on the notions prescribed in the curriculum related to the physical world and (3) on student’s everyday contexts. As illustrations, the following are the erroneous conceptual representations identified in our analysis of the data collected: (1) The change of state of the matter does not require a constant temperature, (2) The temperature is a measure in degrees to indicate the level of heat of an object or person, (3) The mercury contained in a thermometer expands when it is heated so that the particles which constitute it expand and (4) The sensation of cold (or warm) is related to the difference in temperature. In conclusion, we will see that it is possible to develop situations of conflict, dealing specifically with the limits of the analogy between heat and temperature. These situations must consider the conceptual representations of the pre-service teachers, as well as the relevant scientific understanding of the concept of heat and temperature.

Keywords: conceptual representation, heat, temperature, pre-service teachers

Procedia PDF Downloads 105
7629 Application of Functionalized Magnetic Particles as Demulsifier for Oil‐in‐Water Emulsions

Authors: Hamideh Hamedi, Nima Rezaei, Sohrab Zendehboudi

Abstract:

Separating emulsified oil contaminations from waste- or produced water is of interest to various industries. Magnetic particles (MPs) application for separating dispersed and emulsified oil from wastewater is becoming more popular. Stabilization of MPs is required through developing a coating layer on their surfaces to prevent their agglomeration and enhance their dispersibility. In this research, we study the effects of coating material, size, and concentration of iron oxide MPs on oil separation efficiency, using oil adsorption capacity measurements. We functionalize both micro-and nanoparticles of Fe3O4 using sodium dodecyl sulfate (SDS) as an anionic surfactant, cetyltrimethylammonium bromide (CTAB) as a cationic surfactant, and stearic acid (SA). The chemical structures and morphologies of these particles are characterized using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Energy Dispersive X-ray (EDX). The oil-water separation results indicate that a low dosage of the coated magnetic nanoparticle with CTAB (0.5 g/L MNP-CTAB) results the highest oil adsorption capacity (nearly 100%) for 1000 ppm dodecane-in-water emulsion, containing ultra-small droplets (250–300 nm). While separation efficiency of the same dosage of bare MNPs is around 57.5%. Demulsification results of magnetic microparticles (MMPs) also reveal that the functionalizing particles with CTAB increase oil removal efficiency from 86.3% for bare MMP to 92% for MMP-CTAB. Comparing the results of different coating materials implies that the major interaction reaction is an electrostatic attraction between negatively charged oil droplets and positively charged MNP-CTAB and MMP-CTAB. Furthermore, the synthesized nanoparticles could be recycled and reused; after ten cycles the oil adsorption capacity slightly decreases to near 95%. In conclusion, functionalized magnetic particles with high oil separation efficiency could be used effectively in treatment of oily wastewater. Finally, optimization of the adsorption process is required by considering the effective system variables, and fluid properties.

Keywords: oily wastewater treatment, emulsions, oil-water separation, adsorption, magnetic nanoparticles

Procedia PDF Downloads 71
7628 Catalytic Combustion of Methane over Co/Mo and Co/Mn Catalysts at Low Temperature

Authors: Ahmed I. Osman, Jehad K. Abu-Dahrieh, Jillian M. Thompson, David W. Rooney

Abstract:

Natural gas (the main constituent is Methane 95%) is considered as an alternative to petroleum for the production of synthetics fuels. Nowadays, methane combustion at low temperature has received much attention however; it is the most difficult hydrocarbon to be combusted. Co/Mo and (4:1 wt/wt) catalysts were prepared from a range of different precursors and used for the low temperature total methane oxidation (TMO). The catalysts were characterized by, XRD, BET and H2-TPR and tested under reaction temperatures of 250-400 °C with a GHSV= 36,000 mL g-1 h-1. It was found that the combustion temperature was dependent on the type of the precursor, and that those containing chloride led to catalysts with lower activity. The optimum catalyst was Co/Mo (4:1wt/wt) where greater than 20% methane conversion was observed at 250 °C. This catalyst showed a high degree of stability for TMO, showing no deactivation during 50 hours of time on stream.

Keywords: methane low temperature total oxidation, oxygen carrier, Co/Mo, Co/Mn

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7627 Fuzzy Ideal Topological Spaces

Authors: Ali Koam, Ismail Ibedou, S. E. Abbas

Abstract:

In this paper, it is introduced the notion of r-fuzzy ideal separation axioms Tᵢi = 0; 1; 2 based on a fuzzy ideal I on a fuzzy topological space (X; τ). An r-fuzzy ideal connectedness related to the fuzzy ideal I is introduced which has relations with a previous r-fuzzy fuzzy connectedness. An r-fuzzy ideal compactness related to Ι is introduced which has also relations with many other types of fuzzy compactness.

Keywords: fuzzy ideal, fuzzy separation axioms, fuzzy compactness, fuzzy connectedness

Procedia PDF Downloads 225
7626 Fabrication of a High-Performance Polyetherimide Membrane for Helium Separation

Authors: Y. Alqaheem, A. Alomair, F. Altarkait, F. Alswaileh, Nusrat Tanoli

Abstract:

Helium market is continuously growing due to its essential uses in the electronic and healthcare sectors. Currently, helium is produced by cryogenic distillation but the process is uneconomical especially for low production volumes. On the other hand, polymeric membranes can provide a cost-effective solution for helium purification due to their low operating energy. However, the preparation of membranes involves the use of very toxic solvents such as chloroform. In this work, polyetherimide membranes were prepared using a less toxic solvent, n-methylpyrrolidone with a polymer-to-solvent ratio of 27 wt%. The developed membrane showed a superior helium permeability of 15.9 Barrer that surpassed the permeability of membranes made by chloroform.

Keywords: helium separation, polyetherimide, dense membrane, gas permeability

Procedia PDF Downloads 140
7625 3D Numerical Simulation on Annular Diffuser Temperature Distribution Enhancement by Different Twist Arrangement

Authors: Ehan Sabah Shukri, Wirachman Wisnoe

Abstract:

The influence of twist arrangement on the temperature distribution in an annular diffuser fitted with twisted rectangular hub is investigated. Different pitches (Y = 120 mm, 100 mm, 80 mm, and 60 mm) for the twist arrangements are simulated to be compared. The geometry of the annular diffuser and the inlet condition for the hub arrangements are kept constant. The result reveals that using twisted rectangular hub insert with different pitches will force the temperature to distribute in a circular direction. However, temperature distribution will be enhanced with the length pitch increases.

Keywords: numerical simulation, twist arrangement, annular diffuser, temperature distribution, swirl flow, pitches

Procedia PDF Downloads 389
7624 Ammonia Adsorption Properties of Composite Ammonia Carriers Obtained by Supporting Metal Chloride on Porous Materials

Authors: Cheng Shen, LaiHong Shen

Abstract:

Ammonia is an important carrier of hydrogen energy, with the characteristics of high hydrogen content density and no carbon dioxide emission. Ammonia synthesis by the Haber process is the main method for industrial ammonia synthesis, but the conversion rate of ammonia per pass is only about 12%, while the conversion rate of biomass synthesis ammonia is as high as 56%. Therefore, safe and efficient ammonia capture for ammonia synthesis from biomass is an important way to alleviate the energy crisis and solve the energy problem. Metal chloride has a chemical adsorption effect on ammonia, and can be desorbed at high temperature to obtain high-concentration ammonia after combining with ammonia, which has a good development prospect in ammonia capture and separation technology. In this paper, the ammonia adsorption properties of CuCl₂ were measured, and the composite adsorbents were prepared by using silicon and multi-walled carbon nanotubes respectively to support CuCl₂, and the ammonia adsorption properties of the composite adsorbents were studied. The study found that the ammonia adsorption capacity of the three adsorbents decreased with the increase in temperature, so metal chlorides were more suitable for the low-temperature adsorption of ammonia. Silicon and multi-walled carbon nanotubes have an enhanced effect on the ammonia adsorption of CuCl₂. The reason is that the porous material itself has a physical adsorption effect on ammonia, and silicon can play the role of skeleton support in cupric chloride particles, which enhances the pore structure of the adsorbent, thereby alleviating sintering.

Keywords: ammonia, adsorption properties, metal chloride, silicon, MWCNTs

Procedia PDF Downloads 68
7623 Heat Distribution Simulation on Transformer Using FEMM Software

Authors: N. K. Mohd Affendi, T. A. R. Tuan Abdullah, S. A. Syed Mustaffa

Abstract:

In power industry transformer is an important component and most of us familiar by the functioning principle of a transformer electrically. There are many losses occur during the operation of a transformer that causes heat generation. This heat, if not dissipated properly will reduce the lifetime and effectiveness of the transformer. Transformer cooling helps in maintaining the temperature rise of various paths. This paper proposed to minimize the ambient temperature of the transformer room in order to lower down the temperature of the transformer. A simulation has been made using finite element methods programs called FEMM (Finite Elements Method Magnetics) to create a virtual model based on actual measurement of a transformer. The generalization of the two-dimensional (2D) FEMM results proves that by minimizing the ambient temperature, the heat of the transformer is decreased. The modeling process and of the transformer heat flow has been presented.

Keywords: heat generation, temperature rise, ambient temperature, FEMM

Procedia PDF Downloads 356
7622 Synthesis and Characterization of Nickel and Sulphur Sensitized Zinc Oxide Structures

Authors: Ella C. Linganiso, Bonex W. Mwakikunga, Trilock Singh, Sanjay Mathur, Odireleng M. Ntwaeaborwa

Abstract:

The use of nanostructured semiconducting material to catalyze degradation of environmental pollutants still receives much attention to date. One of the desired characteristics for pollutant degradation under ultra-violet visible light is the materials with extended carrier charge separation that allows for electronic transfer between the catalyst and the pollutants. In this work, zinc oxide n-type semiconductor vertically aligned structures were fabricated on silicon (100) substrates using the chemical bath deposition method. The as-synthesized structures were treated with nickel and sulphur. X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy were used to characterize the phase purity, structural dimensions and elemental composition of the obtained structures respectively. Photoluminescence emission measurements showed a decrease in both the near band edge emission as well as the defect band emission upon addition of nickel and sulphur with different concentrations. This was attributed to increased charger-carrier-separation due to the presence of Ni-S material on ZnO surface, which is linked to improved charge transfer during photocatalytic reactions.

Keywords: Carrier-charge-separation, nickel, photoluminescence, sulphur, zinc oxide

Procedia PDF Downloads 275
7621 Collision Avoidance Maneuvers for Vessels Navigating through Traffic Separation Scheme

Authors: Aswin V. J., Sreeja S., R. Harikumar

Abstract:

Ship collision is one of the major concerns while navigating in the ocean. In congested sea routes where there are hectic offshore operations, ships are often forced to take close encounter maneuvers. Maritime rules for preventing collision at sea are defined in the International Regulations for Preventing Collision at Sea. Traffic Separation Schemes (TSS) are traffic management route systems ruled by International Maritime Organization (IMO), where the traffic lanes indicate the general direction of traffic flow. The Rule 10 of International Regulations for Preventing Collision at Sea prescribes the conduct of vessels while navigating through TSS. But no quantitative criteria regarding the procedures to detect and evaluate collision risk is specified in International Regulations for Preventing Collision at Sea. Most of the accidents that occur are due to operational errors affected by human factors such as lack of experience and loss of situational awareness. In open waters, the traffic density is less when compared to that in TSS, and hence the vessels can be operated in autopilot mode. A collision avoidance method that uses the possible obstacle trajectories in advance to predict “collision occurrence” and can generate suitable maneuvers for collision avoidance is presented in this paper. The suitable course and propulsion changes that can be used in a TSS considering International Regulations for Preventing Collision at Sea are found out for various obstacle scenarios.

Keywords: collision avoidance, maneuvers, obstacle trajectories, traffic separation scheme

Procedia PDF Downloads 47
7620 Design, Development and Testing of Polymer-Glass Microfluidic Chips for Electrophoretic Analysis of Biological Sample

Authors: Yana Posmitnaya, Galina Rudnitskaya, Tatyana Lukashenko, Anton Bukatin, Anatoly Evstrapov

Abstract:

An important area of biological and medical research is the study of genetic mutations and polymorphisms that can alter gene function and cause inherited diseases and other diseases. The following methods to analyse DNA fragments are used: capillary electrophoresis and electrophoresis on microfluidic chip (MFC), mass spectrometry with electrophoresis on MFC, hybridization assay on microarray. Electrophoresis on MFC allows to analyse small volumes of samples with high speed and throughput. A soft lithography in polydimethylsiloxane (PDMS) was chosen for operative fabrication of MFCs. A master-form from silicon and photoresist SU-8 2025 (MicroChem Corp.) was created for the formation of micro-sized structures in PDMS. A universal topology which combines T-injector and simple cross was selected for the electrophoretic separation of the sample. Glass K8 and PDMS Sylgard® 184 (Dow Corning Corp.) were used for fabrication of MFCs. Electroosmotic flow (EOF) plays an important role in the electrophoretic separation of the sample. Therefore, the estimate of the quantity of EOF and the ways of its regulation are of interest for the development of the new methods of the electrophoretic separation of biomolecules. The following methods of surface modification were chosen to change EOF: high-frequency (13.56 MHz) plasma treatment in oxygen and argon at low pressure (1 mbar); 1% aqueous solution of polyvinyl alcohol; 3% aqueous solution of Kolliphor® P 188 (Sigma-Aldrich Corp.). The electroosmotic mobility was evaluated by the method of Huang X. et al., wherein the borate buffer was used. The influence of physical and chemical methods of treatment on the wetting properties of the PDMS surface was controlled by the sessile drop method. The most effective way of surface modification of MFCs, from the standpoint of obtaining the smallest value of the contact angle and the smallest value of the EOF, was the processing with aqueous solution of Kolliphor® P 188. This method of modification has been selected for the treatment of channels of MFCs, which are used for the separation of mixture of oligonucleotides fluorescently labeled with the length of chain with 10, 20, 30, 40 and 50 nucleotides. Electrophoresis was performed on the device MFAS-01 (IAI RAS, Russia) at the separation voltage of 1500 V. 6% solution of polydimethylacrylamide with the addition of 7M carbamide was used as the separation medium. The separation time of components of the mixture was determined from electropherograms. The time for untreated MFC was ~275 s, and for the ones treated with solution of Kolliphor® P 188 – ~ 220 s. Research of physical-chemical methods of surface modification of MFCs allowed to choose the most effective way for reducing EOF – the modification with aqueous solution of Kolliphor® P 188. In this case, the separation time of the mixture of oligonucleotides decreased about 20%. The further optimization of method of modification of channels of MFCs will allow decreasing the separation time of sample and increasing the throughput of analysis.

Keywords: electrophoresis, microfluidic chip, modification, nucleic acid, polydimethylsiloxane, soft lithography

Procedia PDF Downloads 384
7619 Nonlinear Heat Transfer in a Spiral Fin with a Period Base Temperature

Authors: Kuo-Teng Tsai, You-Min Huang

Abstract:

In this study, the problem of a spiral fin with a period base temperature is analyzed by using the Adomian decomposition method. The Adomian decomposition method is a useful and practice method to solve the nonlinear energy equation which are associated with the heat radiation. The period base temperature is around a mean value. The results including the temperature distribution and the heat flux from the spiral fin base can be calculated directly. The results also discussed the effects of the dimensionless variables for the temperature variations and the total energy transferred from the spiral fin base.

Keywords: spiral fin, period, adomian decomposition method, nonlinear

Procedia PDF Downloads 498
7618 Aerodynamic Analysis of Dimple Effect on Aircraft Wing

Authors: E. Livya, G. Anitha, P. Valli

Abstract:

The main objective of aircraft aerodynamics is to enhance the aerodynamic characteristics and maneuverability of the aircraft. This enhancement includes the reduction in drag and stall phenomenon. The airfoil which contains dimples will have comparatively less drag than the plain airfoil. Introducing dimples on the aircraft wing will create turbulence by creating vortices which delays the boundary layer separation resulting in decrease of pressure drag and also increase in the angle of stall. In addition, wake reduction leads to reduction in acoustic emission. The overall objective of this paper is to improve the aircraft maneuverability by delaying the flow separation point at stall and thereby reducing the drag by applying the dimple effect over the aircraft wing. This project includes both computational and experimental analysis of dimple effect on aircraft wing, using NACA 0018 airfoil. Dimple shapes of Semi-sphere, hexagon, cylinder, square are selected for the analysis; airfoil is tested under the inlet velocity of 30m/s at different angle of attack (5˚, 10˚, 15˚, 20˚, and 25˚). This analysis favours the dimple effect by increasing L/D ratio and thereby providing the maximum aerodynamic efficiency, which provides the enhanced performance for the aircraft.

Keywords: airfoil, dimple effect, turbulence, boundary layer separation

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7617 Determination of the Thermally Comfortable Air Temperature with Consideration of Individual Clothing and Activity as Preparation for a New Smart Home Heating System

Authors: Alexander Peikos, Carole Binsfeld

Abstract:

The aim of this paper is to determine a thermally comfortable air temperature in an automated living room. This calculated temperature should serve as input for a user-specific and dynamic heating control in such a living space. In addition to the usual physical factors (air temperature, humidity, air velocity, and radiation temperature), individual clothing and activity should be taken into account. The calculation of such a temperature is based on different methods and indices which are usually used for the evaluation of the thermal comfort. The thermal insulation of the worn clothing is determined with a Radio Frequency Identification system. The activity performed is only taken into account indirectly through the generated heart rate. All these methods are ultimately very well suited for use in temperature regulation in an automated home, but still require further research and extensive evaluation.

Keywords: smart home, thermal comfort, predicted mean vote, radio frequency identification

Procedia PDF Downloads 134
7616 Simulation and Experimental Study on Dual Dense Medium Fluidization Features of Air Dense Medium Fluidized Bed

Authors: Cheng Sheng, Yuemin Zhao, Chenlong Duan

Abstract:

Air dense medium fluidized bed is a typical application of fluidization techniques for coal particle separation in arid areas, where it is costly to implement wet coal preparation technologies. In the last three decades, air dense medium fluidized bed, as an efficient dry coal separation technique, has been studied in many aspects, including energy and mass transfer, hydrodynamics, bubbling behaviors, etc. Despite numerous researches have been published, the fluidization features, especially dual dense medium fluidization features have been rarely reported. In dual dense medium fluidized beds, different combinations of different dense mediums play a significant role in fluidization quality variation, thus influencing coal separation efficiency. Moreover, to what extent different dense mediums mix and to what extent the two-component particulate mixture affects the fluidization performance and quality have been in suspense. The proposed work attempts to reveal underlying mechanisms of generation and evolution of two-component particulate mixture in the fluidization process. Based on computational fluid dynamics methods and discrete particle modelling, movement and evolution of dual dense mediums in air dense medium fluidized bed have been simulated. Dual dense medium fluidization experiments have been conducted. Electrical capacitance tomography was employed to investigate the distribution of two-component mixture in experiments. Underlying mechanisms involving two-component particulate fluidization are projected to be demonstrated with the analysis and comparison of simulation and experimental results.

Keywords: air dense medium fluidized bed, particle separation, computational fluid dynamics, discrete particle modelling

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7615 After-Cooling Analysis of RC Structural Members Exposed to High Temperature by Using Numerical Approach

Authors: Ju-Young Hwang, Hyo-Gyoung Kwak

Abstract:

This paper introduces a numerical analysis method for reinforced-concrete (RC) structures exposed to fire and compares the result with experimental results. The proposed analysis method for RC structure under the high temperature consists of two procedures. First step is to decide the temperature distribution across the section through the heat transfer analysis by using the time-temperature curve. After determination of the temperature distribution, the nonlinear analysis is followed. By considering material and geometrical nonlinearity with the temperature distribution, nonlinear analysis predicts the behavior of RC structure under the fire by the exposed time. The proposed method is validated by the comparison with the experimental results. Finally, prediction model to describe the status of after-cooling concrete can also be introduced based on the results of additional experiment. The product of this study is expected to be embedded for smart structure monitoring system against fire in u-City.

Keywords: RC, high temperature, after-cooling analysis, nonlinear analysis

Procedia PDF Downloads 378
7614 Projection of Climate Change over the Upper Ping River Basin Using Regional Climate Model

Authors: Chakrit Chotamonsak, Eric P. Salathé Jr, Jiemjai Kreasuwan

Abstract:

Dynamical downscaling of the ECHAM5 global climate model is applied at 20-km horizontal resolution using the WRF regional climate model (WRF-ECHAM5), to project changes from 1990–2009 to 2045–2064 of temperature and precipitation over the Upper Ping River Basin. The analysis found that monthly changes in daily temperature and precipitation over the basin for the 2045-2064 compared to the 1990-2009 are revealed over the basin all months, with the largest warmer in December and the smallest warmer in February. The future simulated precipitation is smaller than that of the baseline value in May, July and August, while increasing of precipitation is revealed during pre-monsoon (April) and late monsoon (September and October). This means that the rainy season likely becomes longer and less intensified during the rainy season. During the cool-dry season and hot-dry season, precipitation is substantial increasing over the basin. For the annual cycle of changes in daily temperature and precipitation over the upper Ping River basin, the largest warmer in the mean temperature over the basin is 1.93 °C in December and the smallest is 0.77 °C in February. Increase in nighttime temperature (minimum temperature) is larger than that of daytime temperature (maximum temperature) during the dry season, especially in wintertime (November to February), resulted in decreasing the diurnal temperature range. The annual and seasonal changes in daily temperature and precipitation averaged over the basin. The annual mean rising are 1.43, 1.54 and 1.30 °C for mean temperature, maximum temperature and minimum temperature, respectively. The increasing of maximum temperature is larger than that of minimum temperature in all months during the dry season (November to April).

Keywords: climate change, regional climate model, upper Ping River basin, WRF

Procedia PDF Downloads 342
7613 Numerical Investigation on Anchored Sheet Pile Quay Wall with Separated Relieving Platform

Authors: Mahmoud Roushdy, Mohamed El Naggar, Ahmed Yehia Abdelaziz

Abstract:

Anchored sheet pile has been used worldwide as front quay walls for decades. With the increase in vessel drafts and weights, those sheet pile walls need to be upgraded by increasing the depth of the dredging line in front of the wall. A system has recently been used to increase the depth in front of the wall by installing a separated platform supported on a deep foundation (so called Relieving Platform) behind the sheet pile wall. The platform is structurally separated from the front wall. This paper presents a numerical investigation utilizing finite element analysis on the behavior of separated relieve platforms installed within existing anchored sheet pile quay walls. The investigation was done in two steps: a verification step followed by a parametric study. In the verification step, the numerical model was verified based on field measurements performed by others. The validated model was extended within the parametric study to a series of models with different backfill soils, separation gap width, and number of pile rows supporting the platform. The results of the numerical investigation show that using stiff clay as backfill soil (neglecting consolidation) gives better performance for the front wall and the first pile row adjacent to sandy backfills. The degree of compaction of the sandy backfill slightly increases lateral deformations but reduces bending moment acting on pile rows, while the effect is minor on the front wall. In addition, the increase in the separation gap width gradually increases bending moments on the front wall regardless of the backfill soil type, while this effect is reversed on pile rows (gradually decrease). Finally, the paper studies the possibility of reducing the number of pile rows along with the separation to take advantage of the positive separation effect on piles.

Keywords: anchored sheet pile, relieving platform, separation gap, upgrade quay wall

Procedia PDF Downloads 57
7612 An Innovative Use of Flow Columns in Electrocoagulation Reactor to Control Water Temperature

Authors: Khalid S. Hashim, Andy Shaw, Rafid Alkhaddar, David Phipps, Ortoneda Pedrola

Abstract:

Temperature is an essential parameter in the electrocoagulation process (EC) as it governs the solubility of electrodes and the precipitates and the collision rate of particles in water being treated. Although it has been about 100 years since the EC technology was invented and applied in water and wastewater treatment, the effects of temperature on the its performance were insufficiently investigated. Thus, the present project aims to fill this gap by an innovative use of perforated flow columns in the designing of a new EC reactor (ECR1). The new reactor (ECR1) consisted of a Perspex made cylinder container supplied with a flow column consisted of perorated discoid electrodes that made from aluminium. The flow column has been installed vertically, half submerged in the water being treated, inside a plastic cylinder. The unsubmerged part of the flow column works as a radiator for the water being treated. In order to investigate the performance of ECR1; water samples with different initial temperatures (15, 20, 25, 30, and 35 °C) to the ECR1 for 20 min. Temperature of effluent water samples were measured using Hanna meter (Model: HI 98130). The obtained results demonstrated that the ECR1 reduced water temperature from 35, 30, and 25 °C to 24.6, 23.8, and 21.8 °C respectively. While low water temperature, 15 °C, increased slowly to reach 19.1 °C after 15 minutes and kept the same level till the end of the treatment period. At the same time, water sample with initial temperature of 20 °C showed almost a steady level of temperature along the treatment process, where the temperature increased negligibly from 20 to 20.1 °C after 20 minutes of treatment. In conclusion, ECR1 is able to control the temperature of water being treated around the room temperature even when the initial temperature was high (35 °C) or low (15 °C).

Keywords: electrocoagulation, flow column, treatment, water temperature

Procedia PDF Downloads 397