Search results for: process mining
3132 Codon-optimized Carbonic Anhydrase from Dunaliella species: Expression and Characterization
Authors: Seung Pil Pack
Abstract:
Carbonic anhydrases (CAs) has been focused as biological catalysis for CO2 sequestration process because it can catalyze the conversion of CO2 to bicarbonate. Here, codon-optimized sequence of α type-CA cloned from Duneliala species. (DsCAopt) was constructed, expressed, and characterized. The expression level in E. coli BL21(DE3) was better for codon-optimized DsCAopt than intact sequence of DsCAopt. DsCAopt enzyme shows high-stability at pH 7.6/10.0. In final, we demonstrated that in the Ca2+ solution, DsCAopt enzyme can catalyze well the conversion of CO2 to CaCO3, as the calcite form.Keywords: Carbonic anhydrase, Codon-optimization, Duneliala species, CO2 sequestration
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16463131 Enhancing Security in Resource Sharing Using Key Holding Mechanism
Authors: M. Victor Jose, V. Seenivasagam
Abstract:
This paper describes a logical method to enhance security on the grid computing to restrict the misuse of the grid resources. This method is an economic and efficient one to avoid the usage of the special devices. The security issues, techniques and solutions needed to provide a secure grid computing environment are described. A well defined process for security management among the resource accesses and key holding algorithm is also proposed. In this method, the identity management, access control and authorization and authentication are effectively handled.
Keywords: Grid security, Irregular binary series, Key holding mechanism, Resource identity, Secure resource access.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 17253130 Critical Velocities for Particle Transport from Experiments and CFD Simulations
Authors: Sajith Sajeev, Brenton McLaury, Siamack Shirazi
Abstract:
In the petroleum industry, solid particles are often present along with the produced fluids. It is imperative to keep particles from accumulating in flow lines. In this study, various experiments are conducted to study sand particle transport, where critical velocity is defined as the average fluid velocity to keep particles continuously moving. Many parameters related to the fluid, particles and pipe affect the transport process. Experimental results are presented varying the particle concentration. Additionally, CFD simulations using a discrete element modeling (DEM) approach are presented to compare with experimental result.Keywords: Particle transport, critical velocity, CFD, DEM.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12123129 The removal of Ni, Cu and Fe from a Mixed Metal System using Sodium Hypophosphite as a Reducing Agent
Authors: Promise Sethembiso Ngema, Freeman Ntuli, Mohamed Belaid
Abstract:
The main objective of this study was to remove and recover Ni, Cu and Fe from a mixed metal system using sodium hypophosphite as a reducing agent and nickel powder as seeding material. The metal systems studied consisted of Ni-Cu, Ni-Fe and Ni-Cu-Fe solutions. A 5 L batch reactor was used to conduct experiments where 100 mg/l of each respective metal was used. It was found that the metals were reduced to their elemental form with removal efficiencies of over 80%. The removal efficiency decreased in the order Fe>Ni>Cu. The metal powder obtained contained between 97-99% Ni and was almost spherical and porous. Size enlargement by aggregation was the dominant particulate process.
Keywords: crystallization, electroless plating, heavy metal removal, wastewater treatment
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20383128 Calcination Temperature of Nano MgO Effect on Base Transesterification of Palm Oil
Authors: Abdul Rahim Yacob, Mohd Khairul Asyraf Amat Mustajab, Nur Syazeila Samadi
Abstract:
Nano MgO has been synthesized by hydration and dehydration method by modifies the commercial MgO. The prepared MgO had been investigated as a heterogeneous base catalyst for transesterification process for biodiesel production using palm oil. TGA, FT-IR and XRD results obtained from this study lie each other and proved in the formation of nano MgO from decomposition of Mg(OH)2. This study proved that the prepared nano MgO was a better base transesterification catalyst compared to commercial MgO. The nano MgO calcined at 600ºC had gives the highest conversion of 51.3% of palm oil to biodiesel.Keywords: Hydration-dehydration method, nano MgO, transesterification, biodiesel.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 22203127 Research of Concentratibility of Low Quality Bauxite Raw Materials
Authors: Nadezhda Nikolaeva, Tatyana Alexandrova, Alexandr Alexandrov
Abstract:
Processing of high-silicon bauxite on the base of the traditional clinkering method is related to high power consumption and capital investments, which makes production of alumina from those ores non-competitive in terms of basic economic showings. For these reasons, development of technological solutions enabling to process bauxites with various chemical and mineralogical structures efficiently with low level of thermal power consumption is important. Flow sheet of the studies on washability of ores from the Timanskoe and the Severo-Onezhskoe deposits is on the base of the flotation method.Keywords: Low-quality bauxite, resource-saving technology, optimization, aluminum, conditioning of composition, separation characteristics.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19483126 MRI Reconstruction Using Discrete Fourier Transform: A tutorial
Authors: Abiodun M. Aibinu, Momoh J. E. Salami, Amir A. Shafie, Athaur Rahman Najeeb
Abstract:
The use of Inverse Discrete Fourier Transform (IDFT) implemented in the form of Inverse Fourier Transform (IFFT) is one of the standard method of reconstructing Magnetic Resonance Imaging (MRI) from uniformly sampled K-space data. In this tutorial, three of the major problems associated with the use of IFFT in MRI reconstruction are highlighted. The tutorial also gives brief introduction to MRI physics; MRI system from instrumentation point of view; K-space signal and the process of IDFT and IFFT for One and two dimensional (1D and 2D) data.
Keywords: Discrete Fourier Transform (DFT), K-space Data, Magnetic Resonance (MR), Spin, Windows.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 51113125 Fuzzy Multi-Criteria Framework for Supporting Biofuels Policy Making
Authors: Jadwiga R. Ziolkowska
Abstract:
In this paper, a fuzzy algorithm and a fuzzy multicriteria decision framework are developed and used for a practical question of optimizing biofuels policy making. The methodological framework shows how to incorporate fuzzy set theory in a decision process of finding a sustainable biofuels policy among several policy options. Fuzzy set theory is used here as a tool to deal with uncertainties of decision environment, vagueness and ambiguities of policy objectives, subjectivities of human assessments and imprecise and incomplete information about the evaluated policy instruments.Keywords: Fuzzy set theory, multi-criteria decision-makingsupport, uncertainties, policy making, biofuels
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20303124 An Improved Phenomenological Model for Polymer Desorption
Authors: Joanna Sooknanan, Donna Comissiong
Abstract:
We propose a phenomenological model for the process of polymer desorption. In so doing, we omit the usual theoretical approach of incorporating a fictitious viscoelastic stress term into the flux equation. As a result, we obtain a model that captures the essence of the phenomenon of trapping skinning, while preserving the integrity of the experimentally verified Fickian law for diffusion. An appropriate asymptotic analysis is carried out, and a parameter is introduced to represent the speed of the desorption front. Numerical simulations are performed to illustrate the desorption dynamics of the model. Recommendations are made for future modifications of the model, and provisions are made for the inclusion of experimentally determined frontal speeds.Keywords: Phenomenological Model, Polymer, Desorption, Trapping Skinning
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12933123 An Efficient Clustering Technique for Copy-Paste Attack Detection
Authors: N. Chaitawittanun, M. Munlin
Abstract:
Due to rapid advancement of powerful image processing software, digital images are easy to manipulate and modify by ordinary people. Lots of digital images are edited for a specific purpose and more difficult to distinguish form their original ones. We propose a clustering method to detect a copy-move image forgery of JPEG, BMP, TIFF, and PNG. The process starts with reducing the color of the photos. Then, we use the clustering technique to divide information of measuring data by Hausdorff Distance. The result shows that the purposed methods is capable of inspecting the image file and correctly identify the forgery.
Keywords: Image detection, forgery image, copy-paste.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 13193122 FPGA Implementation of the BB84 Protocol
Authors: Jaouadi Ikram, Machhout Mohsen
Abstract:
The development of a quantum key distribution (QKD) system on a field-programmable gate array (FPGA) platform is the subject of this paper. A quantum cryptographic protocol is designed based on the properties of quantum information and the characteristics of FPGAs. The proposed protocol performs key extraction, reconciliation, error correction, and privacy amplification tasks to generate a perfectly secret final key. We modeled the presence of the spy in our system with a strategy to reveal some of the exchanged information without being noticed. Using an FPGA card with a 100 MHz clock frequency, we have demonstrated the evolution of the error rate as well as the amounts of mutual information (between the two interlocutors and that of the spy) passing from one step to another in the key generation process.
Keywords: QKD, BB84, protocol, cryptography, FPGA, key, security, communication.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 8593121 Computing the Similarity and the Diversity in the Species Based on Cronobacter Genome
Authors: E. Al Daoud
Abstract:
The purpose of computing the similarity and the diversity in the species is to trace the process of evolution and to find the relationship between the species and discover the unique, the special, the common and the universal proteins. The proteins of the whole genome of 40 species are compared with the cronobacter genome which is used as reference genome. More than 3 billion pairwise alignments are performed using blastp. Several findings are introduced in this study, for example, we found 172 proteins in cronobacter genome which have insignificant hits in other species, 116 significant proteins in the all tested species with very high score value and 129 common proteins in the plants but have insignificant hits in mammals, birds, fishes, and insects.
Keywords: Genome, species, blastp, conserved genes, cronobacter.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10083120 Forecasting of Grape Juice Flavor by Using Support Vector Regression
Authors: Ren-Jieh Kuo, Chun-Shou Huang
Abstract:
The research of juice flavor forecasting has become more important in China. Due to the fast economic growth in China, many different kinds of juices have been introduced to the market. If a beverage company can understand their customers’ preference well, the juice can be served more attractive. Thus, this study intends to introducing the basic theory and computing process of grapes juice flavor forecasting based on support vector regression (SVR). Applying SVR, BPN, and LR to forecast the flavor of grapes juice in real data shows that SVR is more suitable and effective at predicting performance.
Keywords: Flavor forecasting, artificial neural networks, support vector regression, grape juice flavor.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 22163119 Cognitive Virtual Exploration for Optimization Model Reduction
Authors: Livier Serna, Xavier Fischer, Fouad Bennis
Abstract:
In this paper, a decision aid method for preoptimization is presented. The method is called “negotiation", and it is based on the identification, formulation, modeling and use of indicators defined as “negotiation indicators". These negotiation indicators are used to explore the solution space by means of a classbased approach. The classes are subdomains for the negotiation indicators domain. They represent equivalent cognitive solutions in terms of the negotiation indictors being used. By this method, we reduced the size of the solution space and the criteria, thus aiding the optimization methods. We present an example to show the method.Keywords: Optimization Model Reduction, Pre-Optimization, Negotiation Process, Class-Making, Cognition Based VirtualExploration.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14263118 Preparation and Characterization of Silk/Diopside Composite Nanofibers via Electrospinning for Tissue Engineering Application
Authors: Abbas Teimouri, Leila Ghorbanian, Iren Dabirian
Abstract:
This work focused on preparation and characterizations of silk fibroin (SF)/nanodiopside nanoceramic via electrospinning process. Nanofibrous scaffolds were characterized by combined techniques of scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD). The results confirmed that fabricated SF/diopside scaffolds improved cell attachment and proliferation. The results indicated that the electrospun of SF/nanodiopside nanofibrous scaffolds could be considered as ideal candidates for tissue engineering.Keywords: Electrospinning, nanofibers, silk fibroin, diopside, composite scaffold.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 13053117 Syntax Sensitive and Language Independent Detection of Code Clones
Authors: Kazuaki Maeda
Abstract:
This paper proposes a new technique to detect code clones from the lexical and syntactic point of view, which is based on PALEX source code representation. The PALEX code contains the recorded parsing actions and also lexical formatting information including white spaces and comments. We can record a list of parsing actions (shift, reduce, and reading a token) during a compiling process after a compiler finishes analyzing the source code. The proposed technique has advantages for syntax sensitive approach and language independency.Keywords: Code Clones, Source Code Representation, XML, Parser, Parser Generator
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14613116 A Theoretical Hypothesis on Ferris Wheel Model of University Social Responsibility
Authors: Le Kang
Abstract:
According to the nature of the university, as a free and responsible academic community, USR is based on a different foundation —academic responsibility, so the Pyramid and the IC Model of CSR could not fully explain the most distinguished feature of USR. This paper sought to put forward a new model— Ferris Wheel Model, to illustrate the nature of USR and the process of achievement. The Ferris Wheel Model of USR shows the university creates a balanced, fairness and neutrality systemic structure to afford social responsibilities; that makes the organization could obtain a synergistic effect to achieve more extensive interests of stakeholders and wider social responsibilities.Keywords: USR, Achievement model, Ferris wheel model.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15643115 A New Method for Estimation of the Source Coherency Structure of Wideband Sources
Authors: Yong-jun Zhao, Heng-li Zhang, Zong-yun Hu
Abstract:
Based on the sources- smoothed rank profile (SRP) and modified minimum description length (MMDL) principle, a method for estimation of the source coherency structure (SCS) and the number of wideband sources is proposed in this paper. Instead of focusing, we first use a spatial smoothing technique to pre-process the array covariance matrix of each frequency for de-correlating the sources and then use smoothed rank profile to determine the SCS and the number of wideband sources. We demonstrate the availability of the method by numerical simulations.
Keywords: Wideband sources, source coherency structure (SCS), smoothed rank profile (SRP).
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 13173114 Access Policy Specification for SCADA Networks
Authors: Rodrigo Chandia, Mauricio Papa
Abstract:
Efforts to secure supervisory control and data acquisition (SCADA) systems must be supported under the guidance of sound security policies and mechanisms to enforce them. Critical elements of the policy must be systematically translated into a format that can be used by policy enforcement components. Ideally, the goal is to ensure that the enforced policy is a close reflection of the specified policy. However, security controls commonly used to enforce policies in the IT environment were not designed to satisfy the specific needs of the SCADA environment. This paper presents a language, based on the well-known XACML framework, for the expression of authorization policies for SCADA systems.Keywords: Access policy specification, process control systems, network security.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 23153113 A Multilingual Virtual Simulated Patient Framework for Training Primary Health Care Students
Authors: Juan L. Castro, Maria I. NavarroVictor Lopez, Eduardo M. Eisman, Jose M. Zurita
Abstract:
This paper describes the Multilingual Virtual Simulated Patient framework. It has been created to train the social skills and testing the knowledge of primary health care medical students. The framework generates conversational agents which perform in serveral languages as virtual simulated patients that help to improve the communication and diagnosis skills of the students complementing their training process.
Keywords: Medical training, conversational agents, patient modeling.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15363112 A Rapid Code Acquisition Scheme in OOC-Based CDMA Systems
Authors: Keunhong Chae, Seokho Yoon
Abstract:
We propose a code acquisition scheme called improved multiple-shift (IMS) for optical code division multiple access systems, where the optical orthogonal code is used instead of the pseudo noise code. Although the IMS algorithm has a similar process to that of the conventional MS algorithm, it has a better code acquisition performance than the conventional MS algorithm. We analyze the code acquisition performance of the IMS algorithm and compare the code acquisition performances of the MS and the IMS algorithms in single-user and multi-user environments.
Keywords: Code acquisition, optical CDMA, optical orthogonal code, serial algorithm.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 21573111 Performance Monitoring of the Refrigeration System with Minimum Set of Sensors
Authors: Radek Fisera, Petr Stluka
Abstract:
This paper describes a methodology for remote performance monitoring of retail refrigeration systems. The proposed framework starts with monitoring of the whole refrigeration circuit which allows detecting deviations from expected behavior caused by various faults and degradations. The subsequent diagnostics methods drill down deeper in the equipment hierarchy to more specifically determine root causes. An important feature of the proposed concept is that it does not require any additional sensors, and thus, the performance monitoring solution can be deployed at a low installation cost. Moreover only a minimum of contextual information is required, which also substantially reduces time and cost of the deployment process.Keywords: Condition monitoring, energy baselining, fault detection and diagnostics, commercial refrigeration.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 28803110 Information System Security Effectiveness Attributes: A Tanzanian Company Case Study
Authors: Nerey H. Mvungi, Mosses Makoko
Abstract:
In today-s highly globalised and competitive world access to information plays key role in having an upper hand between business rivals. Hence, proper protection of such crucial resource is core to any modern business. Implementing a successful information security system is basically centered around three pillars; technical solution involving both software and hardware, information security controls to translate the policies and procedure in the system and the people to implement. This paper shows that a lot needs to be done for countries adapting information technology to process, store and distribute information to secure adequately such core resource.Keywords: security, information systems, controls, technology, practices.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 26143109 The Conditioning Effect on Celebrity Multiple Endorsements
Authors: Chia-Ching Tsai
Abstract:
This research adapts experimental design to investigate the effect of conditioning or not and pre-exposure or not on brand attitude, so it is a 2×2=4 factorial design. The results show that the brand attitude of conditioning group is significantly higher than that of unconditioning group. The brand attitude with pre-exposure is significantly higher than that without pre-exposure. Conditioning or not and pre-exposure or not have significant interaction. No matter the celebrity is pre-exposure or not, the brand attitude is higher under conditioning process.Keywords: Celebrity, Multiple endorsements, Pre-exposure, Classical condition, Second-order conditioning
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 29553108 Electrocardiogram Signal Denoising Using a Hybrid Technique
Authors: R. Latif, W. Jenkal, A. Toumanari, A. Hatim
Abstract:
This paper presents an efficient method of electrocardiogram signal denoising based on a hybrid approach. Two techniques are brought together to create an efficient denoising process. The first is an Adaptive Dual Threshold Filter (ADTF) and the second is the Discrete Wavelet Transform (DWT). The presented approach is based on three steps of denoising, the DWT decomposition, the ADTF step and the highest peaks correction step. This paper presents some application of the approach on some electrocardiogram signals of the MIT-BIH database. The results of these applications are promising compared to other recently published techniques.Keywords: Hybrid technique, ADTF, DWT, tresholding, ECG signal.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12003107 Investigation of Some Flotation Parameters and the Role of Dispersants in the Flotation of Chalcopyrite
Authors: H. A. Taner, V. Önen
Abstract:
A suitable choice of flotation parameters and reagents have a strong effect on the effectiveness of flotation process. The objective of this paper is to give an overview of the flotation of chalcopyrite with the different conditions and dispersants. Flotation parameters such as grinding time, pH, type, and dosage of dispersant were investigated. In order to understand the interaction of some dispersants, sodium silicate, sodium hexametaphosphate and sodium polyphosphate were used. The optimum results were obtained at a pH of 11.5 and a grinding time of 10 minutes. A copper concentrate was produced assaying 29.85% CuFeS2 and 65.97% flotation recovery under optimum rougher flotation conditions with sodium silicate.
Keywords: Chalcopyrite, dispersant, flotation, copper.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 7543106 Derivation of Darcy’s Law using Homogenization Method
Authors: Kannanut Chamsri
Abstract:
Darcy’s Law is a well-known constitutive equation describing the flow of a fluid through a porous medium. The equation shows a relationship between the superficial or Darcy velocity and the pressure gradient which was first experimentally observed by Henry Darcy in 1855-1856. In this study, we apply homogenization method to Stokes equation in order to derive Darcy’s Law. The process of deriving the equation is complicated, especially in multidimensional domain. Thus, for the sake of simplicity, we use the indicial notation as well as the homogenization. This combination provides a smooth, simple and easy technique to derive Darcy’s Law.
Keywords: Darcy’s Law, Homogenization method, Indicial notation
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 50183105 Intelligent Agent System Simulation Using Fear Emotion
Authors: Latifeh PourMohammadBagher
Abstract:
In this paper I have developed a system for evaluating the degree of fear emotion that the intelligent agent-based system may feel when it encounters to a persecuting event. In this paper I want to describe behaviors of emotional agents using human behavior in terms of the way their emotional states evolve over time. I have implemented a fuzzy inference system using Java environment. As the inputs of this system, I have considered three parameters related on human fear emotion. The system outputs can be used in agent decision making process or choosing a person for team working systems by combination the intensity of fear to other emotion intensities.Keywords: Emotion simulation, Fear, Fuzzy intelligent agent
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14623104 Removal of Vanadium from Industrial Effluents by Natural Ion Exchanger
Authors: Shashikant R. Kuchekar, Haribhau R. Aher, Priti M. Dhage
Abstract:
The removal vanadium from aqueous solution using natural exchanger was investigated. The effects of pH, contact time and exchanger dose were studied at ambient temperature (25 0C ± 2 0C). The equilibrium process was described by the Langmuir isotherm model with adsorption capacity for vanadium. The natural exchanger i.e. tamarindus seeds powder was treated with formaldehyde and sulpuric acid to increase the adsorptivity of metals. The maximum exchange level was attained as 80.1% at pH 3 with exchanger dose 5 g and contact time 60 min. Method is applied for removal of vanadium from industrial effluents.
Keywords: Industrial effluent, natural ion exchange, Tamarindus indica, vanadium.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 7373103 Automating Test Activities: Test Cases Creation, Test Execution, and Test Reporting with Multiple Test Automation Tools
Authors: Loke Mun Sei
Abstract:
Software testing has become a mandatory process in assuring the software product quality. Hence, test management is needed in order to manage the test activities conducted in the software test life cycle. This paper discusses on the challenges faced in the software test life cycle, and how the test processes and test activities, mainly on test cases creation, test execution, and test reporting is being managed and automated using several test automation tools, i.e. Jira, Robot Framework, and Jenkins.Keywords: Test automation tools, test case, test execution, test reporting.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 3101