Search results for: Endemic species

Authors: Tatyana A. Karpenyuk, Saltanat B. Orazova, Saule A. Dzhokebaeva, Alla V. Goncharova, Yana S. Tzurkan, Alya M. Kalbaeva

Abstract:

It was analyzed of fatty acid composition of 16 strains of microalgae lipid fractions isolated from different basins of Kazakhstan and characterized by stable active growth in the laboratory. Three species of green microalgae (Oocystis rhomboideus, Chlorococcum infusionum, Dictyochlorella globosa) and three species of diatoms (Synedra sp., Nitzshia sp., Pleurosigma attenuatum) are characterized by a high content of lipids and are promising for further study as a source of polyunsaturated fatty acids.

Keywords: Fatty acids, lipids, microalgae.

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Authors: K. V. Pagalthivarthi, R. J. Visintainer

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Two-dimensional Eulerian (volume-averaged) continuity and momentum equations governing multi-size slurry flow through pump casings are solved by applying a penalty finite element formulation. The computational strategy validated for multi-phase flow through rectangular channels is adapted to the present study.   The flow fields of the carrier, mixture and each solids species, and the concentration field of each species are determined sequentially in an iterative manner. The eddy viscosity field computed using Spalart-Allmaras model for the pure carrier phase is modified for the presence of particles. Streamline upwind Petrov-Galerkin formulation is used for all the momentum equations for the carrier, mixture and each solids species and the concentration field for each species. After ensuring mesh-independence of solutions, results of multi-size particulate flow simulation are presented to bring out the effect of bulk flow rate, average inlet concentration, and inlet particle size distribution. Mono-size computations using (1) the concentration-weighted mean diameter of the slurry and (2) the D50 size of the slurry are also presented for comparison with multi-size results.

Keywords: Eulerian-Eulerian model, Multi-size particulate flow, Penalty finite elements, Pump casing, Spalart-Allmaras.

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Authors: Govindaraju.M, Ganeshkumar.R.S, Suganthi.P, Muthukumaran.V.R, Visvanathan.P

Abstract:

The present study was conducted to investigate the response of plants exposed to lignite-based thermal power plant emission. For this purpose, five plant species were collected from 1.0 km distance (polluted site) and control plants were collected from 20.0 km distance (control site) to thermal power plant. The common tree species Cassia siamea Lamk., Polyalthia longifolia. Sonn, Acacia longifolia (Andrews) Wild., Azadirachta indica A.Juss, Ficus religiosa L. were selected as test plants. Photosynthetic pigments changes (chlorophyll a, chlorophyll b and carotenoids) and rubisco enzyme modifications were studied. Reduction was observed in the photosynthetic pigments of plants growing in polluted site and also large sub unit of the rubisco enzyme was degraded in Azadirachta indica A. Juss collected from polluted site.

Keywords: Air pollution, Lignite-based thermal power plant, Photosynthetic pigments, Rubisco enzyme.

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Authors: Xianqing Liu, Shouming Zhong, Lijiang Xiang

Abstract:

In this paper, a stochastic predator-prey system with Bedding-DeAngelis functional response is studied. By constructing a suitable Lyapunov founction, sufficient conditions for species to be stochastically permanent is established. Meanwhile, we show that the species will become extinct with probability one if the noise is sufficiently large.

Keywords: Stochastically permanent, extinct, white noise, Bedding-DeAngelis functional response.

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Authors: Pawan Kumar, Pitamber Singh Negi

Abstract:

Many of the regeneration failures in conifers are often being attributed to heavy insect attack and pathogens during the period of seed formation and under storage conditions. Conifer berries and seed insects occur throughout the known range of the hosts and also limit the production of seed for nursery stock. On occasion, even entire seed crops are lost due to insect attacks. The berry and seeds of both the species have been found to be infected with insects. Recently, heavy damage to the berry and seeds of Juniper and Chilgoza Pine was observed in the field as well as in stored conditions, leading to reduction in the viability of seeds to germinate. Both the species are under great threat and regeneration of the species is very low. Due to lack of adequate literature, the study on the damage potential of seed insects was urgently required to know the exact status of the insect-pests attacking seeds/berries of both the pine species so as to develop pest management practices against the insect pests attack. As both the species are also under threat and are fighting for survival, so the study is important to develop management practices for the insect-pests of seeds/berries of Juniper and Chilgoza pine so as to evaluate in the nursery, as these species form major vegetation of their distribution zones. A six-year study on the management of insect pests of seeds of Chilgoza revealed that seeds of this species are prone to insect pests mainly borers. During present investigations, it was recorded that cones of are heavily attacked only by Dioryctria abietella (Lepidoptera: Pyralidae) in natural conditions, but seeds which are economically important are heavily infected, (sometimes up to 100% damage was also recorded) by insect borer, Plodia interpunctella (Lepidoptera: Pyralidae) and is recorded for the first time ‘to author’s best knowledge’ infesting the stored Chilgoza seeds. Similarly, Juniper berries and seeds were heavily attacked only by a single borer, Homaloxestis cholopis (Lepidoptera: Lecithoceridae) recorded as a new report in natural habitat as well as in stored conditions. During the present investigation details of insect pest attack on Juniper and Chilgoza pine seeds and berries was observed and suitable management practices were also developed to contain the insect-pests attack.

Keywords: Borer, conifer, cones, chilgoza pine, lepidoptera, juniper, management, seed.

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Authors: Yongkun Li, Kaihong Zhao

Abstract:

By using Mawhin-s continuation theorem of coincidence degree theory, we establish the existence of 2n positive periodic solutions for n species non-autonomous Lotka-Volterra competition systems with harvesting terms. An example is given to illustrate the effectiveness of our results.

Keywords: Positive periodic solutions, Lotka-Volterra competition system, coincidence degree, harvesting term.

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Authors: Christine O. Wangia, Jennifer A. Orwa, Francis W. Muregi, Patrick G. Kareru, Kipyegon Cheruiyot, Eric Guantai

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Ruellia (syn. Dipteracanthus) species are wild perennial creepers belonging to the Acanthaceae family. These species are reported to possess anti-inflammatory, analgesic, antioxidant, gastroprotective, anticancer, and immuno-stimulant properties. Phytochemical screening of both aqueous and methanolic extracts of Ruellia species revealed the presence of saponins. Saponins have been reported to possess anti-inflammatory, antioxidant, immuno-stimulant, antihepatotoxic, antibacterial, anticarcinogenic, and antiulcerogenic activities. The objective of this study was to quantify and analyze the Fourier transform infrared (FTIR) spectra of saponins in crude extracts of three Kenyan Ruellia species namely Ruellia prostrata (RPM), Ruellia lineari-bracteolata (RLB) and Ruellia bignoniiflora (RBK). Sequential organic extraction of the ground whole plant material was done using petroleum ether (PE), chloroform, ethyl acetate (EtOAc), and absolute methanol by cold maceration, while aqueous extraction was by hot maceration. The plant powders and extracts were mixed with spectroscopic grade KBr and compressed into a pellet. The infrared spectra were recorded using a Shimadzu FTIR spectrophotometer of 8000 series in the range of 3500 cm^-1 - 500 cm^-1. Quantitative determination of the saponins was done using standard procedures. Quantitative analysis of saponins showed that RPM had the highest quantity of crude saponins (2.05% ± 0.03), followed by RLB (1.4% ± 0.15) and RBK (1.25% ± 0.11), respectively. FTIR spectra revealed the spectral peaks characteristic for saponins in RPM, RLB, and RBK plant powders, aqueous and methanol extracts; O-H absorption (3265 - 3393 cm^-1), C-H absorption ranging from 2851 to 2924 cm^-1, C=C absorbance (1628 - 1655 cm^-1), oligosaccharide linkage (C-O-C) absorption due to sapogenins (1036 - 1042 cm^-1). The crude saponins from RPM, RLB and RBK showed similar peaks to their respective extracts. The presence of the saponins in extracts of RPM, RLB and RBK may be responsible for some of the biological activities reported in the Ruellia species.1

Keywords: Ruellia bignoniiflora, Ruellia lineari-bracteolata, Ruellia prostrata, Saponins.

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Authors: Mohd Iz'aan Paiz Bin Zamri, Anis Salwa Mohd Khairuddin, Norrima Mokhtar, Rubiyah Yusof

Abstract:

Effective statistical feature extraction and classification are important in image-based automatic inspection and analysis. An automatic wood species recognition system is designed to perform wood inspection at custom checkpoints to avoid mislabeling of timber which will results to loss of income to the timber industry. The system focuses on analyzing the statistical pores properties of the wood images. This paper proposed a fuzzy-based feature extractor which mimics the experts’ knowledge on wood texture to extract the properties of pores distribution from the wood surface texture. The proposed feature extractor consists of two steps namely pores extraction and fuzzy pores management. The total number of statistical features extracted from each wood image is 38 features. Then, a backpropagation neural network is used to classify the wood species based on the statistical features. A comprehensive set of experiments on a database composed of 5200 macroscopic images from 52 tropical wood species was used to evaluate the performance of the proposed feature extractor. The advantage of the proposed feature extraction technique is that it mimics the experts’ interpretation on wood texture which allows human involvement when analyzing the wood texture. Experimental results show the efficiency of the proposed method.

Keywords: Classification, fuzzy, inspection system, image analysis.

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Authors: Shu-Ying Marissa Pang, Stephen Tristram, Simon Brown

Abstract:

Fungal infections are becoming more common and the range of susceptible individuals has expanded. While Candida albicans remains the most common infective species, other Candida spp. are becoming increasingly significant. In a range of large-scale studies of candidaemia between 1999 and 2006, about 52% of 9717 cases involved C. albicans, about 30% involved either C. glabrata or C. parapsilosis and less than 15% involved C. tropicalis, C. krusei or C. guilliermondii. However, the probability of mortality within 30 days of infection with a particular species was at least 40% for C. tropicalis, C. albicans, C. glabrata and C. krusei and only 22% for C. parapsilopsis. Clinical isolates of Candida spp. grew at rates ranging from 1.65 h-1 to 4.9 h-1. Three species (C. krusei, C. albicans and C. glabrata) had relatively high growth rates (μm > 4 h-1), C. tropicalis and C. dubliniensis grew moderately quickly (Ôëê 3 h-1) and C. parapsilosis and C. guilliermondii grew slowly (< 2 h-1). Based on these data, the log of the odds of mortality within 30 days of diagnosis was linearly related to μm. From this the underlying probability of mortality is 0.13 (95% CI: 0.10-0.17) and it increases by about 0.09 ± 0.02 for each unit increase in μm. Given that the overall crude mortality is about 0.36, the growth of Candida spp. approximately doubles the rate, consistent with the results of larger case-matched studies of candidaemia.

Keywords: Candida spp., candidiasis, growth, pathogenicity.

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Authors: M M Abubakar, Y M Burrah

Abstract:

Primary and secondary data from the Bauchi abattoir were utilized to determine the relative contributions of different livestock species to meat supply in Bauchi Metropolis. Daily livestock slaughter figures for five months (June – October 2011) indicated that more goats (64.0) were slaughtered than either sheep (47.3) or cattle (41.30) each day (P<0.001) and fewer (P<0.05) animals (45.65) were slaughtered daily in June than either in August (57.07) or October (55.95). Five year (2006 – 2010) monthly slaughter records indicated that both species and year had significant (P<0.001) effects on number of animals slaughtered. More goats (2727.8) were slaughtered than either sheep (731.09) or cattle (520.06) each month and more animals were slaughtered in 2006 (1847.72/month) than in 2008, 2009 and 2010 (997.67, 1029.13 and 1273.75/month respectively). In terms of total carcass output, cattle accounted for 61-80% of the meat supply obtained from ruminant animals slaughtered at the Bauchi abattoir.

Keywords: Carcass Output, Livestock Slaughter Figures, Meat Supply, Ruminant Animals.

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Authors: Farai Nyabadza

Abstract:

Malaria is by far the world-s most persistent tropical parasitic disease and is endemic to tropical areas where the climatic and weather conditions allow continuous breeding of the mosquitoes that spread malaria. A mathematical model for the transmission of malaria with prophylaxis prevention is analyzed. The stability analysis of the equilibria is presented with the aim of finding threshold conditions under which malaria clears or persists in the human population. Our results suggest that eradication of mosquitoes and prophylaxis prevention can significantly reduce the malaria burden on the human population.

Keywords: Prophylaxis prevention, basic reproductive number, stability.

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Authors: Chang-Myeon Lee, Min-Hyung Lee, Jin-Young Hur, Ho-Nyun Lee, Hong-Kee Lee

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An ultrasound-assisted activation method for electroless silver plating is presented in this study. When the ultrasound was applied during the activation step, the amount of the Pd species adsorbed on substrate surfaces was higher than that of sample pretreated with a conventional activation process without ultrasound irradiation. With this activation method, it was also shown that the adsorbed Pd species with a size of about 5 nm were uniformly distributed on the surfaces, thus a smooth and uniform coating on the surfaces was obtained by subsequent electroless silver plating. The samples after each step were characterized by AFM, XPS, FIB, and SEM.

Keywords: Cavitation, Electroless silver, Pd activation, Ultrasonic

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Authors: Richard Elling Moe

Abstract:

Computer languages are usually lumped together into broad -paradigms-, leaving us in want of a finer classification of kinds of language. Theories distinguishing between -genuine differences- in language has been called for, and we propose that such differences can be observed through a notion of expressive mode. We outline this concept, propose how it could be operationalized and indicate a possible context for the development of a corresponding theory. Finally we consider a possible application in connection with evaluation of language revision. We illustrate this with a case, investigating possible revisions of the relational algebra in order to overcome weaknesses of the division operator in connection with universal queries.

Keywords: Expressive mode, Computer language species, Evaluation of revision, Relational algebra, Universal database queries

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Authors: Endrick Barnacin, Jean-Luc Henry, Jack Molinié, Jimmy Nagau, Hélène Delatte, Gérard Lebreton

Abstract:

Palynology is a field of interest for many disciplines. It has multiple applications such as chronological dating, climatology, allergy treatment, and even honey characterization. Unfortunately, the analysis of a pollen slide is a complicated and time-consuming task that requires the intervention of experts in the field, which is becoming increasingly rare due to economic and social conditions. So, the automation of this task is a necessity. Pollen slides analysis is mainly a visual process as it is carried out with the naked eye. That is the reason why a primary method to automate palynology is the use of digital image processing. This method presents the lowest cost and has relatively good accuracy in pollen retrieval. In this work, we propose a system combining recognition and grouping of pollen. It consists of using a Logistic Model Tree to classify pollen already known by the proposed system while detecting any unknown species. Then, the unknown pollen species are divided using a cluster-based approach. Success rates for the recognition of known species have been achieved, and automated clustering seems to be a promising approach.

Keywords: Pollen recognition, logistic model tree, expectation-maximization, local binary pattern.

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Authors: Joserra Díez, Ainhoa Meñika, Iñaki Sanz-Azkue, Arritokieta Ortuzar

Abstract:

Biodiversity provides humans with a great range of ecosystemic services; it is therefore an indispensable resource and a legacy to coming generations. However, in the last decades, the increasing exploitation of the Planet has caused a great loss of biodiversity and its acquaintance has decreased remarkably; especially in urbanized areas, due to the decreasing attachment of humans to nature. Yet, the Primary Education curriculum primes the identification of flora and fauna to guarantee the knowledge of children on their surroundings, so that they care for the environment as well as for themselves. In order to produce effective didactic material that meets the needs of both teachers and pupils, it is fundamental to diagnose the current situation. In the present work, the knowledge on biodiversity of 3^rd cycle Primary Education students in Biscay (n=98) and its relation to the size of the town/city of their school is discussed. Two tests have been used with such aim: one for tree identification and the other one so that the students enumerated the species of trees and animals they knew. Results reveal that knowledge of students on tree identification is scarce regardless the size of the city/town and of their school. On the other hand, animal species are better known than tree species.

Keywords: Biodiversity, population, tree identification, animal identification.

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Authors: Kaihong Zhao

Abstract:

In this paper, the existence of 2n positive periodic solutions for n species non-autonomous Lotka-Volterra cooperative systems with harvesting terms is established by using Mawhin-s continuation theorem of coincidence degree theory and matrix inequality. An example is given to illustrate the effectiveness of our results.

Keywords: Multiple positive periodic solutions, Nonautonomous Lotka-Volterra cooperative system, Coincidence degree, Harvesting term.

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Authors: Anuj K. Sharma, Amit Sharma, Kulbhushan Agnihotri

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In this work, we propose and analyze a model of Phytoplankton-Zooplankton interaction with harvesting considering that some species are exploited commercially for food. Criteria for local stability, instability and global stability are derived and some threshold harvesting levels are explored to maintain the population at an appropriate equilibrium level even if the species are exploited continuously.Further,biological and bionomic equilibria of the system are obtained and an optimal harvesting policy is also analysed using the Pantryagin’s Maximum Principle.Finally analytical findings are also supported by some numerical simulations.

Keywords: Phytoplankton-Zooplankton, Global stability, Bionomic Equilibrium, Pontrying-Maximum Principal.

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Authors: Hasmik P. Ter-Voskanyan

Abstract:

The account of genus Polygonum L. in "Flora of Armenia" was made more than five decades ago. After that many expeditions have been carried out in different regions of Armenia and a huge herbarium material has been collected. The genus included 5 sections with 20 species. Since then many authors accepted the sections as separate genera on the basis of anatomical, morphological, palynological and molecular data. According to the above mentioned it became clear, that the taxonomy of Armenian representatives of Polygonum s. l. also needs revision. New literature data and our investigations of live and herbarium material (ERE, LE) with specification of the morphological characters, distribution, ecology, flowering and fruiting terms brought us to conclusion, that genus Polygonum s. l. has to be split into 5 different genera (Aconogonon (Meisn.) Reichenb., Bistorta (L.) Scop., Fallopia Adans., Persicaria Mill., Polygonum L. s. s.). The number of species has been reduced to 16 species. For each genus new determination keys has been created. 

Keywords: Aconogonon (Meisn.) Reichenb., Bistorta (L.) Scop., Fallopia Adans., Persicaria Mill., Polygonum L. s. s., Flora of Armenia.

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Authors: Urszula Wachowska, Anna Daria Stasiulewicz-Paluch, Katarzyna Kucharska

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A three-year field experiment (2010-2012) was conducted to determine the abundance of epiphytic and endophytic filamentous fungi colonizing the grain of winter wheat cv. Bogatka. Wheat spikes were protected with tebuconazole or chitosan at the watery ripe stage. Untreated plants served as control. Tebuconazole exerted an inhibitory effect primarily on F. culmorum and F. graminearum, and its effectiveness was determined by the pressure from pathogens that infected wheat spikes during the growing season. Chitosan did not suppress the growth of Fusarium species and Alternaria alternata.

Keywords: Winter wheat, tebuconazole, chitosan, Fusarium.

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Authors: Endrick Barnacin, Jean-Luc Henry, Jimmy Nagau, Jack Molinié

Abstract:

Palynology is a field of interest in many disciplines due to its multiple applications: chronological dating, climatology, allergy treatment, and honey characterization. Unfortunately, the analysis of a pollen slide is a complicated and time consuming task that requires the intervention of experts in the field, which are becoming increasingly rare due to economic and social conditions. In this context, the automation of this task is urgent. In this work, we compare classical feature extraction methods (Shape, GLCM, LBP, and others) and Deep Learning (CNN and Transfer Learning) to perform a recognition task over 80 regional pollen species. It has been found that the use of Transfer Learning seems to be more precise than the other approaches.

Keywords: Image segmentation, stuck particles separation, Sobel operator, thresholding.

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Authors: Abdesselem Dakhli, Wajdi Bellil, Chokri Ben Amar

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DNA Barcode provides good sources of needed information to classify living species. The classification problem has to be supported with reliable methods and algorithms. To analyze species regions or entire genomes, it becomes necessary to use the similarity sequence methods. A large set of sequences can be simultaneously compared using Multiple Sequence Alignment which is known to be NP-complete. However, all the used methods are still computationally very expensive and require significant computational infrastructure. Our goal is to build predictive models that are highly accurate and interpretable. In fact, our method permits to avoid the complex problem of form and structure in different classes of organisms. The empirical data and their classification performances are compared with other methods. Evenly, in this study, we present our system which is consisted of three phases. The first one, is called transformation, is composed of three sub steps; Electron-Ion Interaction Pseudopotential (EIIP) for the codification of DNA Barcodes, Fourier Transform and Power Spectrum Signal Processing. Moreover, the second phase step is an approximation; it is empowered by the use of Multi Library Wavelet Neural Networks (MLWNN). Finally, the third one, is called the classification of DNA Barcodes, is realized by applying the algorithm of hierarchical classification.

Keywords: DNA Barcode, Electron-Ion Interaction Pseudopotential, Multi Library Wavelet Neural Networks.

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Authors: R. Kongnuy, P. Pongsumpun

Abstract:

Dengue, a disease found in most tropical and subtropical areas of the world. It has become the most common arboviral disease of humans. This disease is caused by any of four serotypes of dengue virus (DEN1-DEN4). In many endemic countries, the average age of getting dengue infection is shifting upwards, dengue in pregnancy and infancy are likely to be encountered more frequently. The dynamics of the disease is studied by a compartmental model involving ordinary differential equations for the pregnant, infant human and the vector populations. The stability of each equilibrium point is given. The epidemic dynamic is discussed. Moreover, the numerical results are shown for difference values of dengue antibody.

Keywords: Dengue antibody, infant, pregnant human, mathematical model.

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Authors: Weiwen Li, Hao Huang, Chengmao You, Jianji Liao, Lei Wang, Lina An

Abstract:

Zooplankton are a central issue in the ecology which makes a great contribution to maintaining the balance of an ecosystem. It is critical in promoting the material cycle and energy flow within the ecosystems. A generalized additive model (GAM) was applied to analyze the relationships between the density (individuals per m³) of zooplankton and other variables in West Daya Bay. All data used in this analysis (the survey month, survey station (longitude and latitude), the depth of the water column, the superficial concentration of chlorophyll a, the benthonic concentration of chlorophyll a, the number of zooplankton species and the number of zooplankton species) were collected through monthly scientific surveys during January to December 2016. GLM model (generalized linear model) was used to choose the significant variables’ impact on the density of zooplankton, and the GAM was employed to analyze the relationship between the density of zooplankton and the significant variables. The results showed that the density of zooplankton increased with an increase of the benthonic concentration of chlorophyll a, but decreased with a decrease in the depth of the water column. Both high numbers of zooplankton species and the overall total number of zooplankton individuals led to a higher density of zooplankton.

Keywords: Density, generalized linear model, generalized additive model, the West Daya Bay, zooplankton.

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Authors: Yeanchon H. Dulanlebit, Nikmans Hattu, Gloria Bora

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Seaweed is a type of macro algae which are good source of iodine and have been widely used as food and nutrition supplement. One of iodine species that found in ocean plant is iodate. Analysis of iodate in seaweed (Eucheuma cottonii) from coastal area of Maluku has been done. The determination is done by using spectrophotometric method. Iodate in sample is reduced in excess of potassium iodide in the presence of acid solution, and then is reacted with starch to form blue complex. The study found out that the highest wavelength on determination of iodate species using spectrophotometer analysis method is 570 nm. Optimum value to yield maximum absorption is used in this research. Contents of iodate in seawater from coastal area of Ambon Island, Western Seram and Southeast Maluku are 0.2655, 0.2719 and 0.1760 mg/L, respectively. While in seaweeds from Ambon Island, Western Seram, Southeast Maluku-Taar, Ohoidertawun and Wab are 6.3122, 6.3293, 6.2333, 3.7406 and 4.4207 mg/kg in dry weight. Bioconcentration (enrichment) factor of iodate in seaweed (Eucheuma cottonii) from the three samples (cluster) is different; in Coastal area of Ambon Island, Western Seram and Southeast Maluku respectively are 23.78, 23.28 and 27.26.

Keywords: Bioconcentration, Eucheuma cottonii, iodate, iodine, seaweed.

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Authors: M. Grifoni, G. Petruzzelli, M. Barbafieri, I. Rosellini, B. Pezzarossa, F. Pedron

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Evapotranspiration (ET) covers are an alternative cover system that utilizes water balance approach to maximize the ET process to reduce the contaminants leaching through the soil profile. Microcosm tests allow to identify in a short time the most suitable plant species to be used as alternative covers, their survival capacity, and simultaneously the transpiration and evaporation rate of the cover in a specific contaminated soil. This work shows the soil characterization and ET results of microcosm tests carried out on two contaminated soils by using Triticum durum and Helianthus annuus species. The data indicated that transpiration was higher than evaporation, supporting the use of plants as alternative cover at this contaminated site.

Keywords: Contaminated sites, ET cover, evapotranspiration, microcosm experiments.

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Authors: Christian A. Romero, Tanja Grkovic, John. R. J. French, D. İpek. Kurtböke, Ronald J. Quinn

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A high-throughput and automated 1H NMR metabolic fingerprinting dereplication approach was used to accelerate the discovery of unknown bioactive secondary metabolites. The applied dereplication strategy accelerated the discovery of new natural products, provided rapid and competent identification and quantification of the known secondary metabolites and avoided time-consuming isolation procedures. The effectiveness of the technique was demonstrated by the isolation and elucidation of arglecins B (1), C (2) and actinofuranosin A (3) from a termite-gut associated Streptomyces sp. (USC 597) grown under solid state fermentation. The structures of these compounds were elucidated by extensive interpretation of 1H, 13C and 2D NMR spectroscopic data. These represent the first report of arglecin analogues isolated from a termite gut-associated Streptomyces species.

Keywords: Actinomycetes, actinofuranosin, antibiotics, arglecins, NMR spectroscopy.

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Authors: M.N. Khan, T. Rehman, Z Iqbal, M.S Sajid, M Ahmad, M Riaz

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A cross-sectional study was carried out to determine the prevalence, species characterization and associated risk factors with Eimeria (E.) in sheep of district Toba Tek Singh from April, 2009 to March, 2010. Of the total 486 faecal samples examined for Eimeria, 209 (43%) were found infected with five species of Eimeria. Amongst the identified species of Eimeria, E. ovinoidalis was the commonest one (48.32%), followed in order by E. ahsata, E. intricata, E. parva and E. faurei with prevalence of 45.45, 28.71, 24.40 and 19.14 percent respectively. Peak prevalence was observed in August. Wet season (rainy and post-rainy) was found to be favourable for Eimeria infection. Lambs had significantly higher prevalence (P < 0.05) of Eimeria than adults. Similarly higher prevalence of Eimeria was observed in female as compared to male. Among management and husbandry practices; watering system, housing system, floor type and herd size strongly influenced the prevalence of Eimeria. Coccidiosis was more prevalent in closed housing system, non-cemented floor type, pond watered animals and larger herds (P < 0.05) as compared to open housing system, partially cemented floor type, tap watered animals and smaller herds respectively. Feeding system, breed and body condition of animals were not found as risk factors (P>0.05) influencing prevalence of Eimeria.

Keywords: Eimeria, Pakistan prevalence, sheep.

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Authors: S. A. A. Korori, H. Valipour K., S. Shabestani, A. shirvany, M. Matinizadeh

Abstract:

Tehran, one of the heavily-populated capitals, is severely suffering from increasing air pollution. To show a documented trend of such pollutants during last years, plane tree species (Platanus orientalis) were suited to be studied as indicators, for the species have been planted throughout the city many years ago. Two areas (Saadatabad and Narmak districts) allotting different contents of crowed and highly-traffic routs but the same ecological characteristics were selected. Twelve sample individuals were cored twice perpendicularly in each area. Tree-rings of each core were measured by a binocular microscope and separated annually for the last 25 years. Two heavy metals including Cd and Pb accompanied by a mineral element (Ca) were analyzed using Hatch method. Treerings analysis of the two areas showed different groups in term of physiologically ability as the growths were plunged during the last 10 years in Saadatabad district and showed a slight decrease in the same period for another studying area. In direct contrast to decreasing growth trend in Saadatabad, all three mentioned elements increased sharply during last 25 years in the same area. When it came to Narmak district, the trend was completely different with Saadatabad. There were some fluctuations in absorbing trace elements like tree-rings widths were, yet calcium showed an upward trend all the last 25 years. The results of the study proved the possibility of using tree species of each region to monitor its air pollution trends of the past, hence to depict a pollution assessment of a populated city for last years and then to make appropriate decisions for the future as it is well-known what the trend is. On the other hand, risen values of calcium (as the stress-indicator element) accompanied by increased trace elements suggests non-sustainable state of the trees.

Keywords: Air pollution, Platanus orientalis, Tehran, Traceelements, Tree rings.

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Authors: Wanyu Mao, Qiwen Sun, Weiyong Ying, Dingye Fang

Abstract:

The influence of copper promoters and reaction conditions on the formation of alcohols byproducts of a common Fischer-Tropsch synthesis used iron-based catalysts were investigated. A good compromise of 28%Cu/FeKLaSiO2 can lead to the optimization of an improved Fischer-Tropsch catalyst. The product distribution shifts towards hydrocarbons with increasing the reaction temperature, while pressure promotes the formation of alcohols. It was found that the production of either alcohols or hydrocarbons followed A-S-F distributions, and their α parameters were essentially different which indicated a competition in the growing chain between the two species. TPD after acetaldehyde adsorption gave strong evidence of the insertion of a C1 oxygen-containing species into an alkyl chain.

Keywords: Fischer-Tropsch synthesis, Fe-Cu catalyst, alcohols byproducts, reaction pathways

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Authors: Zuzana Poborilova, Anna B. Ohlsson, Torkel Berglund, Anna Vildova, Petr Babula

Abstract:

Lawsone is a pigment that occurs naturally in plants. It has been used as a skin and hair dye for a long time. Moreover, its different biological activities have been reported. The present study focused on the effect of lawsone on a plant cell model represented by tobacco BY-2 cell suspension culture, which is used as a model comparable with the HeLa cells. It has been shown that lawsone inhibits the cell growth in the concentration-dependent manner. In addition, changes in DNA methylation level have been determined. We observed decreasing level of DNA methylation in the presence of increasing concentrations of lawsone. These results were accompanied with overproduction of reactive oxygen species (ROS). Since epigenetic modifications can be caused by different stress factors, there could be a connection between the changes in the level of DNA methylation and ROS production caused by lawsone.

Keywords: DNA methylation, Lawsone, Naphthoquinone, Reactive Oxygen Species.

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