Search results for: Java code generation

Authors: A. Biran, P. Sobhe Bidari, A. Almazroe V. Lakshminarayanan, K. Raahemifar

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Diabetic Retinopathy (DR) is a severe retinal disease which is caused by diabetes mellitus. It leads to blindness when it progress to proliferative level. Early indications of DR are the appearance of microaneurysms, hemorrhages and hard exudates. In this paper, an automatic algorithm for detection of DR has been proposed. The algorithm is based on combination of several image processing techniques including Circular Hough Transform (CHT), Contrast Limited Adaptive Histogram Equalization (CLAHE), Gabor filter and thresholding. Also, Support Vector Machine (SVM) Classifier is used to classify retinal images to normal or abnormal cases including non-proliferative or proliferative DR. The proposed method has been tested on images selected from Structured Analysis of the Retinal (STARE) database using MATLAB code. The method is perfectly able to detect DR. The sensitivity specificity and accuracy of this approach are 90%, 87.5%, and 91.4% respectively.

Keywords: Diabetic retinopathy, fundus images, STARE, Gabor filter, SVM.

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Authors: Cătălin Mărculescu, Andrei Avram, Cătălin Pârvulescu, Marioara Avram, Cătălin Mihai Bălan

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The present microfluidic study is emphasizing the flow behavior within a Y shape micro-bifurcation in two similar flow configurations. We report here a numerical and experimental investigation on the velocity profiles evolution and secondary flows, manifested at different Reynolds numbers (Re) and for two different boundary conditions. The experiments are performed using special designed setup based on optical microscopic devices. With this setup, direct visualizations and quantitative measurements of the path-lines are obtained. A Micro-PIV measurement system is used to obtain velocity profiles distributions in a spatial evolution in the main flows domains. The experimental data is compared with numerical simulations performed with commercial computational code FLUENT in a 3D geometry with the same dimensions as the experimental one. The numerical flow patterns are found to be in good agreement with the experimental manifestations.

Keywords: Micro-PIV, numerical investigations, secondary flows, velocity profiles.

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Authors: Hemanth Kumar, A. R., Sudhakar G, Satyanarayana B. S.

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In this paper, Land Marks for Unique Addressing( LMUA) algorithm is develped to generate unique ID for each and every node which leads to the formation of overlapping/Non overlapping clusters based on unique ID. To overcome the draw back of the developed LMUA algorithm, the concept of clustering is introduced. Based on the clustering concept a Land Marks for Unique Addressing and Clustering(LMUAC) Algorithm is developed to construct strictly non-overlapping clusters and classify those nodes in to Cluster Heads, Member Nodes, Gate way nodes and generating the Hierarchical code for the cluster heads to operate in the level one hierarchy for wireless communication switching. The expansion of the existing network can be performed or not without modifying the cost of adding the clusterhead is shown. The developed algorithm shows one way of efficiently constructing the

Keywords: Cluster Dimension, Cluster Basis, Metric Dimension, Metric Basis.

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Authors: Pauliasi Tony Fakahau

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This paper provides an overview of the impact of the revenue reform programme on vulnerable communities in the Kingdom of Tonga. Economic turmoil and mismanagement during the late 1990s forced the government to seek technical and financial assistance from the Asian Development Bank to undertake a comprehensive Economic and Public Sector Reform (EPSR) programme. The EPSR is a Western model recommended by donor agencies as the solution to Tonga’s economic challenges. The EPSR programme included public sector reform, private sector growth, and revenue generation. Tax reform was the main tool for revenue generation, which set out to strengthen tax compliance and administration as well as implement a value-added consumption tax. The EPSR is based on Western values and ideology but failed to recognise that Tongan cultural values are important to the local community. Two participant groups were interviewed. Participant group one consisted of 51 people representing vulnerable communities. Participant group two consisted of six people from the government and business sector who were from the elite of Tongan society. The Kakala Research Methodology provided the framework for the research, and the Talanoa Research Method was used to conduct semi-structured interviews in the homes of the first group and in the workplaces of the second group. The research found a heavy burden of the consumption tax on the purchasing power of participant group one (vulnerable participants), having an impact on nearly every financial transaction they made. Participant group one’s main financial priorities were kavenga fakalotu (obligations to the church), kavenga fakafāmili (obligations to the family) and kavenga fakafonua (obligations to cultural events for the village, nobility, and royalty). The findings identified inequalities of the revenue reform, especially from consumption tax, for vulnerable people and communities compared to the elite of society. The research concluded that government and donor agencies need ameliorating policies to reduce the burden of tax on vulnerable groups more susceptible to the impact of revenue reform.

Keywords: Tax reform, Tonga vulnerable community revenue, revenue reform, public sector reform.

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Authors: Prashanth C.M., K. Chandrashekar Shet

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In recent past, the Unified Modeling Language (UML) has become the de facto industry standard for object-oriented modeling of the software systems. The syntax and semantics rich UML has encouraged industry to develop several supporting tools including those capable of generating deployable product (code) from the UML models. As a consequence, ensuring the correctness of the model/design has become challenging and extremely important task. In this paper, we present an approach for automatic verification of protocol model/design. As a case study, Session Initiation Protocol (SIP) design is verified for the property, “the CALLER will not converse with the CALLEE before the connection is established between them ". The SIP is modeled using UML statechart diagrams and the desired properties are expressed in temporal logic. Our prototype verifier “UML-SMV" is used to carry out the verification. We subjected an erroneous SIP model to the UML-SMV, the verifier could successfully detect the error (in 76.26ms) and generate the error trace.

Keywords: Unified Modeling Language, Statechart, Verification, Protocol Design, Model Checking.

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Authors: Woo Young Jung, Bu Seog Ju

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This study described the seismic performance evaluation of bridge structures, located near Daegu metropolitan city in Korea. The structural design code or regulatory guidelines is focusing on the protection of brittle failure or collapse in bridges’ lifetime during an earthquake. This paper illustrated the procedure in terms of the safety evaluation of bridges using simple linear elastic 3D Finite Element (FE) model in ABAQUS platform. The design response spectra based on KBC 2009 were then developed, in order to understand the seismic behavior of bridge structures. Besides, the multiple directional earthquakes were applied and it revealed that the most dominated earthquake direction was transverse direction of the bridge. Also, the bridge structure under the compressive stress was more fragile than the tensile stress and the vertical direction of seismic ground motions was not significantly affected to the structural system.

Keywords: Bridge, Finite Element, 3D model, Earthquake, Spectrum.

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Authors: Matthew Montebello

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The way e-learning effectiveness has been notoriously measured within an academic setting is by comparing the e-learning medium to the traditional face-to-face teaching methodology. In this paper, a simple yet innovative comparison methodology is introduced, whereby the effectiveness of next generation e-learning systems are assessed in contrast not only to the face-to-face mode, but also to the classical e-learning modality. Ethical and logistical issues are also discussed, as this three-way approach to compare teaching methodologies was applied and documented in a real empirical study within a higher education institution.

Keywords: E-learning effectiveness, higher education, teaching modality comparison.

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Authors: Musa H. Arslan, Murat Ceylan, Tayfun Koyuncu

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In this study, an Artificial Neural Network (ANN) analytical method has been developed for analyzing earthquake performances of the Reinforced Concrete (RC) buildings. 66 RC buildings with four to ten storeys were subjected to performance analysis according to the parameters which are the existing material, loading and geometrical characteristics of the buildings. The selected parameters have been thought to be effective on the performance of RC buildings. In the performance analyses stage of the study, level of performance possible to be shown by these buildings in case of an earthquake was determined on the basis of the 4-grade performance levels specified in Turkish Earthquake Code-2007 (TEC-2007). After obtaining the 4-grade performance level, selected 23 parameters of each building have been matched with the performance level. In this stage, ANN-based fast evaluation algorithm mentioned above made an economic and rapid evaluation of four to ten storey RC buildings. According to the study, the prediction accuracy of ANN has been found about 74%.

Keywords: Artificial neural network, earthquake, performance, reinforced concrete.

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Authors: Richard Thai-Singama, Jean-Pierre Belin, Frédéric Du Burck, Marc Piette

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This paper presents a simple and original method for the generation of short monocycle pulses based on the transient response of a passive band-pass filter. The recorded sub-nanosecond pulses show a good symmetry and a small ringing (13 % of the peak amplitude). Their spectral density covers the range 3.1 GHz to 10.6 GHz. The possibility to adapt the pulse spectral density to the indoor FCC frequency mask is demonstrated with a prototype working at a reduced frequency (FCC/1000). A detection technique is proposed.

Keywords: Impulse, Monocycle, Transient, UWB.

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Authors: Hossein Shokouhmand , Koohyar Vahidkhah, Mohammad A. Esmaeili

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A numerical investigation of surface heat transfer characteristics of turbulent air flows in different parallel plate grooved channels is performed using CFD code. The results are obtained for Reynolds number ranging from 10,000 to 30,000 and for arc-shaped and rectangular grooved channels. The influence of different geometric parameters of dimples as well as the number of them and the geometric and thermophysical properties of channel walls are studied. It is found that there exists an optimum value for depth of dimples in which the largest wall heat flux can be achieved. Also, the results show a critical value for the ratio of wall thermal conductivity to the one of fluid in which the dependence of wall heat flux to this ratio almost vanishes. In most cases examined, heat transfer enhancement is larger for arc-shaped grooved channels than rectangular ones.

Keywords: dimple, heat transfer enhancement, Numerical, optimum value, turbulent air flow.

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Authors: Makhlouf Mourad, Medkour Mihoub, Bouchher Omar, Messabih Sidi Mohamed, Benrachedi Khaled

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This work is the modeling and simulation of fluid flow (liquid) through porous media. This type of flow occurs in many situations of interest in applied sciences and engineering, fluid (oil) consists of several individual substances in pure, single-phase flow is incompressible and isothermal. The porous medium is isotropic, homogeneous optionally, with the rectangular format and the flow is two-dimensional. Modeling of hydrodynamic phenomena incorporates Darcy's law and the equation of mass conservation. Correlations are used to model the density and viscosity of the fluid. A finite volume code is used in the discretization of differential equations. The nonlinearity is treated by Newton's method with relaxation coefficient. The results of the simulation of the pressure and the mobility of liquid flowing through porous media are presented, analyzed, and illustrated.

Keywords: Darcy equation, middle porous, continuity equation, Peng Robinson equation, mobility.

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Authors: P. S. Bedi, Neayti Thakur, Balvinder Singh

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In the present study an attempt has been made to prepare the crude extracts of leaves and stem of ‘Girardinia heterophylla’ by using various solvents like petroleum ether, ethanol and double distilled water. The samples were given the code NGLS 1, NGLS 2, NGLS 3 and NGSS 1, NGSS 2 and NGSS 3 respectively. All the extracts were used to study their antimicrobial activity against gram positive bacteria e.g. Bacillus subtilis, gram negative bacteria e.g. E. coli and K. pneumonia and antifungal activity against Aspergillus niger. The results of the antimicrobial activity showed that all the crude extracts of the plant possesses antibacterial activity. Maximum antibacterial activity was shown by NGLS 2, NGLS 3 and NGSS 3 against K. pneumonia. The growth of fungus A. niger was also inhibited by all the crude extracts. Maximum inhibition was shown by NGSS 2 followed by NGSS 1.

Keywords: Girardinia heterophylla, leaves and stem extracts, antibacterial activity, antifungal activity.

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Authors: Chan Hee Park, Arim Lee, Jung Min Lee, Joo Hyun Moon

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Accident in spent fuel pool (SFP) of Fukushima Daiichi Unit 4 showed the importance of continuous monitoring of the key environmental parameters such as water temperature, water level, and radiation level in the SFP at accident conditions. Because the SFP water temperature is one of the key parameters indicating SFP conditions, its behavior at accident conditions shall be understood to prepare appropriate measures. This study estimated temporal change in the SFP water temperature at Kori Unit 1 with 587 MWe for 1 hour after initiation of a loss-of-pool-cooling accident. For the estimation, ANSYS CFX 13.0 code was used. The estimation showed that the increasing rate of the water temperature was 3.90C per hour and the SFP water temperature could reach 1000C in 25.6 hours after the initiation of loss-of-pool-cooling accident.

Keywords: Spent fuel pool, water temperature, Kori Unit 1, a loss-of-pool-cooling accident.

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Authors: M. A. Sharbafi, M. Shakiba Herfeh, Caro Lucas, A. Mohammadi Nejad

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Several researchers have proposed methods about combination of Genetic Algorithm (GA) and Fuzzy Logic (the use of GA to obtain fuzzy rules and application of fuzzy logic in optimization of GA). In this paper, we suggest a new method in which fuzzy decision making is used to improve the performance of genetic algorithm. In the suggested method, we determine the alleles that enhance the fitness of chromosomes and try to insert them to the next generation. In this algorithm we try to present an innovative vaccination in the process of reproduction in genetic algorithm, with considering the trade off between exploration and exploitation.

Keywords: Genetic Algorithm, Fuzzy Decision Making.

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Authors: J. Fernandez de Canete, P. Del Saz Orozco, S. Gonzalez-Perez

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LabVIEW and SIMULINK are two most widely used graphical programming environments for designing digital signal processing and control systems. Unlike conventional text-based programming languages such as C, Cµ and MATLAB, graphical programming involves block-based code developments, allowing a more efficient mechanism to build and analyze control systems. In this paper a LabVIEW environment has been employed as a graphical user interface for monitoring the operation of a controlled distillation column, by visualizing both the closed loop performance and the user selected control conditions, while the column dynamics has been modeled under the SIMULINK environment. This tool has been applied to the PID based decoupled control of a binary distillation column. By means of such integrated environments the control designer is able to monitor and control the plant behavior and optimize the response when both, the quality improvement of distillation products and the operation efficiency tasks, are considered.

Keywords: Distillation control, software tools, SIMULINKLabVIEWinterface.

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Authors: Mahmoud Miri, Morteza Naghipour, Amir Kashiryfar

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The unanticipated destruct of more of the steel moment frames in Northridge earthquake, altered class of regard to the beamto- column connections in moment frames. Panel zone is one the significant part of joints which, it-s stiffness and rigidity has an important effect on the behavior and ductility of the frame. Specifically that behavior of panel zone has a very significant effect on the special moment frames. In this paper , meanwhile the relations for modeling of panel zone in frames are expressed , special moment frames with different spans and stories were studied in the way of performance-based design. The frames designed in according with Iranian steel building code. The effect of panel zone is also considered and in the case of non-existence of performance level, by changing in intimacies and parameter of panel zone, performance level is considered.

Keywords: steel moment frame, panel zone, performance based design

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Authors: Ishrar Tabassum, Jay Andrew Saptok, Khalid Raihan Kabir, Elmee Tabassum

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The purpose of this study is to bring light to the current state of the working environments in the corporate environment and other such institutions with a particular focus on the Bangladesh National Building Code (BNBC) and its guidelines for accommodating the physically disabled. Data were collected via semi-formal interviews, site visits and focus groups conducted using a preset questionnaire as the guidelines. After conducting surveys at corporate offices of 20 organizations from major commercial sectors in Dhaka city, the auditing showed many inadequacies, as aside from the larger corporate offices, the offices have little to no accessibility for the physically disabled. This study hopes to shed light on the fact that the existing BNBCs lack of emphasis on ensuring the accessibility of the handicapped in corporate buildings in the hope that, in the future, the physically disabled will have greater opportunities at being productive members of the workforce.

Keywords: Person with disability, PWD, corporate buildings, Dhaka City.

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Authors: P. Phokharatkul, K. Sankhuangaw, S. Somkuarnpanit, S. Phaiboon, C. Kimpan

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Much research into handwritten Thai character recognition have been proposed, such as comparing heads of characters, Fuzzy logic and structure trees, etc. This paper presents a system of handwritten Thai character recognition, which is based on the Ant-minor algorithm (data mining based on Ant colony optimization). Zoning is initially used to determine each character. Then three distinct features (also called attributes) of each character in each zone are extracted. The attributes are Head zone, End point, and Feature code. All attributes are used for construct the classification rules by an Ant-miner algorithm in order to classify 112 Thai characters. For this experiment, the Ant-miner algorithm is adapted, with a small change to increase the recognition rate. The result of this experiment is a 97% recognition rate of the training set (11200 characters) and 82.7% recognition rate of unseen data test (22400 characters).

Keywords: Hand written, Thai character recognition, Ant-mineralgorithm, distinct feature.

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Authors: Alvise Pellegrini, Ernesto Benini

Abstract:

The design of a steam turbine is a very complex engineering operation that can be simplified and improved thanks to computer-aided multi-objective optimization. This process makes use of existing optimization algorithms and losses correlations to identify those geometries that deliver the best balance of performance (i.e. Pareto-optimal points). This paper deals with a one-dimensional multi-objective and multi-point optimization of a single-stage steam turbine. Using a genetic optimization algorithm and an algebraic one-dimensional ideal gas-path model based on loss and deviation correlations, a code capable of performing the optimization of a predefined steam turbine stage was developed. More specifically, during this study the parameters modified (i.e. decision variables) to identify the best performing geometries were solidity and angles both for stator and rotor cascades, while the objective functions to maximize were totalto- static efficiency and specific work done. Finally, an accurate analysis of the obtained results was carried out.

Keywords: Steam turbine, optimization, genetic algorithms.

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Authors: Craig Comstock, Zhizhong Jiang, Peter Naudé

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Productivity has been one of the major concerns with the increasingly high cost of software development. Choosing the right development language with high productivity is one approach to reduce development costs. Working on the large database with 4106 projects ever developed, we found the factors significant to productivity. After the removal of the effects of other factors on productivity, we compare the productivity differences of the ten general development programs. The study supports the fact that fourth-generation languages are more productive than thirdgeneration languages.

Keywords: Functional point, language, productivity, software engineering.

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Authors: Carol B.Collins, M. H. N Tabrizi

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The purposes of this paper are to (1) promote excellence in computer science by suggesting a cohesive innovative approach to fill well documented deficiencies in current computer science education, (2) justify (using the authors- and others anecdotal evidence from both the classroom and the real world) why this approach holds great potential to successfully eliminate the deficiencies, (3) invite other professionals to join the authors in proof of concept research. The authors- experiences, though anecdotal, strongly suggest that a new approach involving visual modeling technologies should allow computer science programs to retain a greater percentage of prospective and declared majors as students become more engaged learners, more successful problem-solvers, and better prepared as programmers. In addition, the graduates of such computer science programs will make greater contributions to the profession as skilled problem-solvers. Instead of wearily rememorizing code as they move to the next course, students will have the problem-solving skills to think and work in more sophisticated and creative ways.

Keywords: Algorithms, CASE, Problem-solving, UML.

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Authors: F. Mehran

Abstract:

In this paper, we study a class of serially concatenated block codes (SCBC) based on matrix interleavers, to be employed in fixed wireless communication systems. The performances of SCBC¬coded systems are investigated under various interleaver dimensions. Numerical results reveal that the matrix interleaver could be a competitive candidate over conventional block interleaver for frame lengths of 200 bits; hence, the SCBC coding based on matrix interleaver is a promising technique to be employed for speech transmission applications in many international standards such as pan-European Global System for Mobile communications (GSM), Digital Cellular Systems (DCS) 1800, and Joint Detection Code Division Multiple Access (JD-CDMA) mobile radio systems, where the speech frame contains around 200 bits.

Keywords: Matrix Interleaver, serial concatenated block codes (SCBC), turbo codes, wireless communications.

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Authors: Amit Sachan

Abstract:

The purpose of this paper is to acquire the remote electrical parameters like Voltage, Current, and Frequency from Smart grid and send these real time values over GSM network using GSM Modem/phone along with temperature at power station. This project is also designed to protect the electrical circuitry by operating an Electromagnetic Relay. The Relay can be used to operate a Circuit Breaker to switch off the main electrical supply. User can send commands in the form of SMS messages to read the remote electrical parameters. This system also can automatically send the real time electrical parameters periodically (based on time settings) in the form of SMS. This system also send SMS alerts whenever the Circuit Breaker trips or whenever the Voltage or Current exceeds the predefined limits.

Keywords: GSM Modem, Initialization of ADC module of microcontroller, PIC-C compiler for Embedded C programming, PIC kit 2 programmer for dumping code into Micro controller, Express SCH for Circuit design, Proteus for hardware simulation.

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Authors: S. Pesina, T. Solonchak

Abstract:

Phenomenological analysis is not based on natural language, but ideal language which is able to be a carrier of ideal meanings – eidos representing typical structures or essences. For this purpose, it’s necessary to release from the spatio-temporal definiteness of a subject and then state its noetic essence (eidos) by means of free fantasy generation. Herewith, as if a totally new objectness is created - the universal, confirming the thesis that thinking process takes place in generalizations passing by numerous means through the specific to the general and from the general through the specific to the singular.

Keywords: Lexical eidos, phenomenology, noema, polysemantic word, semantic core.

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Authors: J. P. Dubois

Abstract:

Single side band modulation is a widespread technique in communication with significant impact on communication technologies such as DSL modems and ATSC TV. Its widespread utilization is due to its bandwidth and power saving characteristics. In this paper, we present a new scheme for SSB signal generation which is cost efficient and enjoys superior characteristics in terms of frequency stability, selectivity, and robustness to noise. In the process, we develop novel Hilbert transform properties.

Keywords: Crystal filter, frequency drift, frequency mixing, Hilbert transform, phasing, selectivity, single side band AM.

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Authors: Ahmed ElShafee, Karim Alaa Hamed

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This paper presents a design and prototype implementation of new home automation system that uses WiFi technology as a network infrastructure connecting its parts. The proposed system consists of two main components; the first part is the server (web server), which presents system core that manages, controls, and monitors users- home. Users and system administrator can locally (LAN) or remotely (internet) manage and control system code. Second part is hardware interface module, which provides appropriate interface to sensors and actuator of home automation system. Unlike most of available home automation system in the market the proposed system is scalable that one server can manage many hardware interface modules as long as it exists on WiFi network coverage. System supports a wide range of home automation devices like power management components, and security components. The proposed system is better from the scalability and flexibility point of view than the commercially available home automation systems.

Keywords: Home automation, Wireless LAN, WiFi, MicroControllers

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Authors: G. Sai Kiran , A. Kailasa Rao, R . Pradeep Kumar

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In recent years, the introduction of Pre Engineered Building (PEB) concept in the design of structures has helped in optimizing design. The adoptability of PEB in the place of Conventional Steel Building (CSB) design concept resulted in many advantages, including economy and easier fabrication. In this study, an industrial structure (Ware House) is analyzed and designed according to the Indian standards, IS 800-1984, IS 800-2007 and also by referring MBMA-96 and AISC-89. In this study, a structure with length 187m,width 40m,with clear height 8m and having R-Slope 1:10,isconsidered to carry out analysis& design for 2D frames (End frame, frame without crane and frame with 3 module cranes). The economy of the structure is discussed in terms of its weight comparison, between Indian codes (IS800-1984, IS800-2007) & American code (MBMA-96), & between Indian codes (IS800-1984, IS800-2007).

Keywords: AISC, Crane Beam, MBMA, Pre-Engineered-Buildings, Staad Pro, Utilization Ratio.

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Authors: J. R. Wang, Y. Chiang, W. S. Hsu, H. C. Chen, S. H. Chen, J. H. Yang, S. W. Chen, C. Shih

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In this study, the RASCAL and ALOHA codes are used to establish an analysis methodology for hydrogen fluoride (HF) evaluation. There are three main steps in this study. First, the UF₆ data were collected. Second, one postulated case was analyzed by using the RASCAL and UF₆ data. This postulated case assumes that fire occurring and UF₆ is releasing from a building. Third, the results of RASCAL for HF mass were as the input data of ALOHA. Two postulated cases of HF were analyzed by using ALOHA code and the results of RASCAL. These postulated cases assume fire occurring and HF is releasing with no raining (Case 1) or raining (Case 2) condition. According to the analysis results of ALOHA, the HF concentration of Case 2 is smaller than Case 1. The results can be a reference for the preparing of emergency plans for the release of HF.

Keywords: RASCAL, ALOHA, UF6, hydrogen fluoride.

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Authors: M. Raciti Castelli, A. Castelli, E. Benini

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The flow field over a flat roof model building has been numerically investigated in order to determine threedimensional CFD guidelines for the calculation of the turbulent flow over a structure immersed in an atmospheric boundary layer. To this purpose, a complete validation campaign has been performed through a systematic comparison of numerical simulations with wind tunnel experimental data. Wind tunnel measurements and numerical predictions have been compared for five different vertical positions, respectively from the upstream leading edge to the downstream bottom edge of the analyzed model. Flow field characteristics in the neighborhood of the building model have been numerically investigated, allowing a quantification of the capabilities of the CFD code to predict the flow separation and the extension of the recirculation regions. The proposed calculations have allowed the development of a preliminary procedure to be used as guidance in selecting the appropriate grid configuration and corresponding turbulence model for the prediction of the flow field over a three-dimensional roof architecture dominated by flow separation.

Keywords: CFD, roof, building, wind

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Authors: T. Sadunishvili, E. Kvesitadze, M. Betsiashvili, N. Kuprava, G. Zaalishvili, G. Kvesitadze

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Influence of octane and benzene on plant cell ultrastructure and enzymes of basic metabolism, such as nitrogen assimilation and energy generation have been studied. Different plants: perennial ryegrass (Lolium perenne) and alfalfa (Medicago sativa); crops- maize (Zea mays L.) and bean (Phaseolus vulgaris); shrubs – privet (Ligustrum sempervirens) and trifoliate orange (Poncirus trifoliate); trees - poplar (Populus deltoides) and white mulberry (Morus alba L.) were exposed to hydrocarbons of different concentrations (1, 10 and 100 mM). Destructive changes in bean and maize leaves cells ultrastructure under the influence of benzene vapour were revealed at the level of photosynthetic and energy generation subcellular organells. Different deviations at the level of subcellular organelles structure and distribution were observed in alfalfa and ryegrass root cells under the influence of benzene and octane, absorbed through roots. The level of destructive changes is concentration dependent. Benzene at low 1 and 10 mM concentration caused the increase in glutamate dehydrogenase (GDH) activity in maize roots and leaves and in poplar and mulberry shoots, though to higher extent in case of lower, 1mM concentration. The induction was more intensive in plant roots. The highest tested 100mM concentration of benzene was inhibitory to the enzyme in all plants. Octane caused induction of GDH in all grassy plants at all tested concentrations; however the rate of induction decreased parallel to increase of the hydrocarbon concentration. Octane at concentration 1 mM caused induction of GDH in privet, trifoliate and white mulberry shoots. The highest, 100mM octane was characterized by inhibitory effect to GDH activity in all plants. Octane had inductive effect on malate dehydrogenase in almost all plants and tested concentrations, indicating the intensification of Trycarboxylic Acid Cycle. The data could be suggested for elaboration of criteria for plant selection for phytoremediation of oil hydrocarbons contaminated soils.

Keywords: Higher plants, hydrocarbons, cell ultrastructure, glutamate and malate dehydrogenases.

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