Search results for: waterlily shape

Authors: Mohammad Asif

Abstract:

Heterogeneous catalysis is vital for a number of chemical, refinery and pollution control processes. The use of catalyst pellets of hollow cylindrical shape provide several distinct advantages over other common shapes, and can therefore help to enhance conversion levels in reactors. A better utilization of the catalytic material is probably most notable of these features due to the absence of the pellet core, which helps to significantly lower the effect of the internal transport resistance. This is reflected in the enhancement of the effectiveness factor. For the case of the first order irreversible kinetics, a substantial increase in the effectiveness factor can be obtained by varying shape parameters. Important shape parameters of a finite hollow cylinder are the ratio of the inside to the outside radii (κ) and the height to the diameter ratio (γ). A high value of κ the generally helps to enhance the effectiveness factor. On the other hand, lower values of the effectiveness factors are obtained when the dimension of the height and the diameter are comparable. Thus, the departure of parameter γ from the unity favors higher effectiveness factor. Since a higher effectiveness factor is a measure of a greater utilization of the catalytic material, higher conversion levels can be achieved using the hollow cylindrical pellets possessing optimized shape parameters.

Keywords: Finite hollow cylinder, Catalyst pellet, Effectiveness factor, Thiele Modulus, Conversion

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Authors: M. Malekyarand, M. Shariati Ghalehno, A. Mokhber Dezfuli, H. Saebi Monfared, S. R. Ghoraishi K.

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Shape and form of the watermelon fruits are important factors to save spaces and reducing damage during storing of the fruits. In order to save spaces and prevent fruit damage in watermelon the following experiment was carried out in the farm. The fruits were boxed when they were approximately one cm less than the box diameter. The cubic, hexagonal forms were compared in this research. To do this, different boxes were designed with different holes on the sides to holes the watermelons fruits for shaping. The shapes of the boxes were hexagonal and cubic. The boxes holes sizes were the same with 10mm diameter each. Each side of the boxes had different holes including: without holes to 75 holes. The result showed that the best shape for watermelon storing to save space and prevent fruit damage was hexagonal form. The percentages of the fruit damage were 33 to 80 respectively.

Keywords: Cubic form, fruit damage, hexagonal, watermelon shape.

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Authors: Elmi Abu Bakar, Tetsuo Miyake, Zhong Zhang, Takashi Imamura

Abstract:

Knowing the geometrical object pose of products in manufacturing line before robot manipulation is required and less time consuming for overall shape measurement. In order to perform it, the information of shape representation and matching of objects is become required. Objects are compared with its descriptor that conceptually subtracted from each other to form scalar metric. When the metric value is smaller, the object is considered closed to each other. Rotating the object from static pose in some direction introduce the change of value in scalar metric value of boundary information after feature extraction of related object. In this paper, a proposal method for indexing technique for retrieval of 3D geometrical models based on similarity between boundaries shapes in order to measure 3D CAD object pose using object shape feature matching for Computer Aided Testing (CAT) system in production line is proposed. In experimental results shows the effectiveness of proposed method.

Keywords: CAD, rendering, feature extraction, feature classification.

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Authors: L. Fridrichova, R. Knížek, V. Bajzík

Abstract:

Drape is one of the important visual characteristics of the fabric. This paper is introducing an innovative method of measurement and evaluation of the drape shape of the fabric. The measuring principle is based on the possibility of multiple vertical strain of the fabric. This method more accurately simulates the real behavior of the fabric in the process of draping. The method is fully automated, so the sample can be measured by using any number of cycles in any time horizon. Using the present method of measurement, we are able to describe the viscoelastic behavior of the fabric.

Keywords: Drape, drape shape, automated drape meter.

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Authors: Seyed Mohyeddin Ghodratian, Mehdi Ghassemieh, Mohammad Khanmohammadi

Abstract:

Excessive ductility demand on shorter piers is a common problem for irregular bridges subjected to strong ground motion. Various techniques have been developed to reduce the likelihood of collapse of bridge due to failure of shorter piers. This paper presents the new approach to improve the seismic behavior of such bridges using Nitinol shape memory alloys (SMAs). Superelastic SMAs have the ability to remain elastic under very large deformation due to martensitic transformation. This unique property leads to enhanced performance of controlled bridge compared with the performance of the reference bridge. To evaluate the effectiveness of the devices, nonlinear time history analysis is performed on a RC single column bent highway bridge using a suite of representative ground motions. The results show that this method is very effective in limiting the ductility demand of shorter pier.

Keywords: bridge, ductility demand, irregularity, shape memory alloy

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Authors: Nasim Daemi, Gholam Hossein Majzoobi

Abstract:

In this work, bending fatigue life of notched specimens with various notch geometries and dimensions is investigated by experiment and Manson-Caffin theoretical method. In this theoretical method, fatigue life of notched specimens is calculated using the fatigue life obtained from the experiments for plain specimens (without notch). Three notch geometries including ∪-shape, ∨-shape and C -shape notches are considered in this investigation. The experiments are conducted on a rotary bending Moore machine. The specimens are made of a low carbon steel alloy, which has wide application in industry. The stress- life curves are captured for all notched specimen by experiment. The results indicate that Manson-Caffin analytical method cannot adequately predict the fatigue life of notched specimen. However, it seems that the difference between the experiments and Manson-Caffin predictions can be compensated by a proportional factor.

Keywords: fatigue life, Mason-Caffin method, notchedspecimen, stress-life curve.

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Authors: Seong Do Kim, Woo Young Jung

Abstract:

Recently, damages due to typhoons and strong wind are on the rise. Considering this issue, we evaluated the performance of soundproofing walls based on the strong wind fragility by means of numerical analysis. Among the components of the soundproof wall, aluminum frame was the most vulnerable member, thus we have considered different section of aluminum frame in the determination of wind fragility. Wind load was randomly generated using Monte Carlo Simulation method. Moreover, limit state was based on the test standard of road construction soundproofing wall. In this study, the strong wind fragility was determined by considering the influence factors of wind exposure category, soundproof wall’s installation position, and shape of aluminum frame section. Results of this study could be used to determine the section shape of the frame that has high resistance to the wind during construction of the soundproofing wall.

Keywords: Aluminum frame soundproofing wall, Monte Carlo Simulation, numerical simulation, wind fragility.

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Authors: A. Armin, R. Fotouhi, W. Szyszkowski

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In this paper a 3-D finite element (FE) investigation of soil-blade interaction is described. The effects of blade’s shape and rake angle are examined both numerically and experimentally. The soil is considered as an elastic-plastic granular material with non-associated Drucker-Prager material model. Contact elements with different properties are used to mimic soil-blade sliding and soil-soil cutting phenomena. A separation criterion is presented and a procedure to evaluate the forces acting on the blade is given and discussed in detail. Experimental results were derived from tests using soil bin facility and instruments at the University of Saskatchewan. During motion of the blade, load cells collect data and send them to a computer. The measured forces using load cells had noisy signals which are needed to be filtered. The FE results are compared with experimental results for verification. This technique can be used in blade shape optimization and design of more complicated blade’s shape.

Keywords: Finite element analysis, soil-blade contact modeling, blade force, experimental results.

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Authors: Henri Champliaud, Zhengkun Feng, Ngan Van Lê, Javad Gholipour

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In this article, a method is presented to effectively estimate the deformed shape of a thick plate due to line heating. The method uses a fifth order spline interpolation, with up to C3 continuity at specific points to compute the shape of the deformed geometry. First and second order derivatives over a surface are the resulting parameters of a given heating line on a plate. These parameters are determined through experiments and/or finite element simulations. Very accurate kriging models are fitted to real or virtual surfaces to build-up a database of maps. Maps of first and second order derivatives are then applied on numerical plate models to evaluate their evolving shapes through a sequence of heating lines. Adding an optimization process to this approach would allow determining the trajectories of heating lines needed to shape complex geometries, such as Francis turbine blades.

Keywords: Deformation, kriging, fifth order spline interpolation, first, second and third order derivatives, C3 continuity, line heating, plate forming, thermal forming.

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Authors: R. Kanmaniraja, R. Freshipali, J. Abdullah, K. Niranjan, K. Balasubramani, V. R. Sanal Kumar

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The present environmental issues have made aircraft jet noise reduction a crucial problem in aero-acoustics research. Acoustic studies reveal that addition of chevrons to the nozzle reduces the sound pressure level reasonably with acceptable reduction in performance. In this paper comprehensive numerical studies on acoustic characteristics of different types of chevron nozzles have been carried out with non-reacting flows for the shape optimization of chevrons in supersonic nozzles for aerospace applications. The numerical studies have been carried out using a validated steady 3D density based, k-ε turbulence model. In this paper chevron with sharp edge, flat edge, round edge and U-type edge are selected for the jet acoustic characterization of supersonic nozzles. We observed that compared to the base model a case with round-shaped chevron nozzle could reduce 4.13% acoustic level with 0.6% thrust loss. We concluded that the prudent selection of the chevron shape will enable an appreciable reduction of the aircraft jet noise without compromising its overall performance. It is evident from the present numerical simulations that k-ε model can predict reasonably well the acoustic level of chevron supersonic nozzles for its shape optimization.

Keywords: Supersonic nozzle, Chevron, Acoustic level, Shape Optimization of Chevron Nozzles, Jet noise suppression.

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Authors: M. Hakak Khadem, S. Hossainpour, M. Shams

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A numerical simulation of micro Poiseuille flow has performed for rarefied and compressible flow at slip flow regimes. The wall roughness is simulated in two cases with triangular microelements and random micro peaks distributed on wall surfaces to study the effects of roughness shape and distribution on flow field. Two values of Mach and Knudsen numbers have used to investigate the effects of rarefaction as well as compressibility. The numerical results have also checked with available theoretical and experimental relations and good agreements has achieved. High influence of roughness shape can be seen for both compressible and incompressible rarefied flows. In addition it is found that rarefaction has more significant effect on flow field in microchannels with higher relative roughness. It is also found that compressibility has more significant effects on Poiseuille number when relative roughness increases.

Keywords: Relative roughness, slip flow, Poiseuille number, roughness distribution.

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Authors: Reimund Neugebauer, Welf-Guntram Drossel, Andre Bucht, Christoph Ohsenbrügge

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In this paper the authors propose and verify an approach to control heat flow in machine tool components. Thermal deformations are a main aspect that affects the accuracy of machining. Due to goals of energy efficiency, thermal basic loads should be reduced. This leads to inhomogeneous and time variant temperature profiles. To counteract these negative consequences, material with high melting enthalpy is used as a method for thermal stabilization. The increased thermal capacity slows down the transient thermal behavior. To account for the delayed thermal equilibrium, a control mechanism for thermal flow is introduced. By varying a gap in a heat flow path the thermal resistance of an assembly can be controlled. This mechanism is evaluated in two experimental setups. First to validate the ability to control the thermal resistance and second to prove the possibility of a self-sufficient option based on the selfsensing abilities of thermal shape memory alloys.

Keywords: energy-efficiency, heat transfer path, MT thermal stability, thermal shape memory alloy

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Authors: Lenka Ocenasova, Bartosz Gapinski, Robert Cep, Linda Gregova, Branimir Barisic, Jana Novakova, Lenka Petrkovska

Abstract:

Today technological process makes possible surface control of producing parts which is needful for product quality guarantee. Geometrical structure of part surface includes form, proportion, accuracy to shape, accuracy to size, alignment and surface topography (roughness, waviness, etc.). All these parameters are dependence at technology, production machine parameters, material properties, but also at human, etc. Every parameters approves at total part accuracy, it is means at accuracy to shape. One of the most important accuracy to shape element is roundness. This paper will be deals by comparison of roughness deviations at coordination measuring machines and at special single purpose machines. Will describing measuring by discreet method (discontinuous) and scanning method (continuous) at coordination measuring machines and confrontation with reference method using at single purpose machines.

Keywords: Coordinating Measuring Machines (CMM), Measuring Strategy, Roughness Deviation, Accuracy.

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Authors: Fathi M. O. Hamed, Salma F. Elkofhaifee

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The aim of this work is to detect geometrical shape objects in an image. In this paper, the object is considered to be as a circle shape. The identification requires find three characteristics, which are number, size, and location of the object. To achieve the goal of this work, this paper presents an algorithm that combines from some of statistical approaches and image analysis techniques. This algorithm has been implemented to arrive at the major objectives in this paper. The algorithm has been evaluated by using simulated data, and yields good results, and then it has been applied to real data.

Keywords: Image processing, median filter, projection, scalespace, segmentation, threshold.

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Authors: Takashi Ueda, Shinichi Enoki

Abstract:

Forging parts is used to automobiles; because, they have high strength and it is possible to press them into complicated shape. When itis possible to manufacture hollow forging parts, it leads to reduce weightof the automobiles. But, hollow forging parts are confined to axisymmetrical shape. Hollowforging parts that were pressed to complicated shape are expected. Therefore, we forge a blank that aluminum alloy was inserted in stainless steel. After that, we can providecomplex forging parts that are reduced weight,ifit is possible to be melted the aluminum alloy away by using different of melting points.It is necessary to establish heat forging analysis methodon blank consist of stainless steel and aluminum alloy. Because,this forging is different from conventional forging and this technology is not confirmed. In this study, we compared forging experiment with numerical analysis on the view point of forming load and shape after forming and establish how to set the material temperaturesof two metals and material property of stainless steel on the analysis method. Consequently, temperature difference of stainless steel and aluminum alloy was obtained by experiment. We got material property of stainless steel on forging experimental by compression tests. We had compared numerical analysis that was used the temperature difference of two metals and the material property of stainless steel on forging experimental with forging experiment. Forging analysis method on blankconsist of two metals was established by result of numerical analysis having agreedwith result of forging experiment.

Keywords: Forging, lightweight, analysis, hollow.

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Authors: J. Hassan

Abstract:

Water 2H NMR signal on the surface of nano-silica material, MCM-41, consists of two overlapping resonances. The 2H water spectrum shows a superposition of a Lorentzian line shape and the familiar NMR powder pattern line shape, indicating the existence of two spin components. Chemical exchange occurs between these two groups. Decomposition of the two signals is a crucial starting point for study the exchange process. In this article we have determined these spin component populations along with other important parameters for the 2H water NMR signal over a temperature range between 223 K and 343 K.

Keywords: Nano-Silica, surface water, NMR

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Authors: A.M. Fahim, A. M. Salem, F. A. Torkey, M. A. Ramadan

Abstract:

Clustering algorithms are attractive for the task of class identification in spatial databases. However, the application to large spatial databases rises the following requirements for clustering algorithms: minimal requirements of domain knowledge to determine the input parameters, discovery of clusters with arbitrary shape and good efficiency on large databases. The well-known clustering algorithms offer no solution to the combination of these requirements. In this paper, a density based clustering algorithm (DCBRD) is presented, relying on a knowledge acquired from the data by dividing the data space into overlapped regions. The proposed algorithm discovers arbitrary shaped clusters, requires no input parameters and uses the same definitions of DBSCAN algorithm. We performed an experimental evaluation of the effectiveness and efficiency of it, and compared this results with that of DBSCAN. The results of our experiments demonstrate that the proposed algorithm is significantly efficient in discovering clusters of arbitrary shape and size.

Keywords: Clustering Algorithms, Arbitrary Shape of clusters, cluster Analysis.

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Authors: Bongsoo Jeon, Jayoung Kim, Jihong Lee

Abstract:

Methods for measuring or estimating ground shape by a laser range finder and a vision sensor (Exteroceptive sensors) have critical weaknesses in terms that these methods need a prior database built to distinguish acquired data as unique surface conditions for driving. Also, ground information by Exteroceptive sensors does not reflect the deflection of ground surface caused by the movement of UGVs. Therefore, this paper proposes a method of recognizing exact and precise ground shape using an Inertial Measurement Unit (IMU) as a proprioceptive sensor. In this paper, firstly this method recognizes the attitude of a robot in real-time using IMU and compensates attitude data of a robot with angle errors through analysis of vehicle dynamics. This method is verified by outdoor driving experiments of a real mobile robot.

Keywords: Inertial Measurement Unit, Laser Range Finder, Real-time recognition of the ground shape.

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Authors: Farshad Mehrabi, Ali kheirodin, Mohsen Gerami

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Ambiguities in effects of earthquake on various structures in all earthquake codes would necessitate more study and research concerning influential factors on dynamic behavior. Previous studies which were done on different features in different buildings play a major role in the type of response a structure makes to lateral vibrations. Diagnosing each of these irregularities can help structure designers in choosing appropriate setbacks for decreasing possible damages. Therefore vertical setback is one of the irregularity factors in the height of the building where can be seen in skyscrapers and hotels. Previous researches reveal notable changes in the place of these setbacks showing dynamic response of the structure. Consequently analyzing 48 models of concrete frames for 3, 6 and 9 stories heights with three different bays in general shape of a surface decline by height have been constructed in ETABS2000 software, and then the shape effect of each and every one of these frames in period scale has been discussed. The result of this study reveals that not only mass, stiffness and height but also shape of the frame is influential.

Keywords: period, concrete frame, irregularity in height, decrease in plan surface, dynamic behavior

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Authors: Kanda Wongwailikhit

Abstract:

Copper (I) oxide microparticles with the morphology of cubic and hollow sphere were synthesized with the assistance of surfactant as the shape controller. Both particles were then subjected to study the catalytic activity and observed the results of shape effects of catalysts on rate of catalytic reaction. The decolorizing reaction of crystal violet and sodium hydroxide was chosen and measured the decreasing of reactant with respect to times using spectrophotometer. The result revealed that morphology of crystal had no effect on the catalytic activity for crystal violet reaction but contributed to total surface area predominantly.

Keywords: Copper (I) oxide, Catalytic activity, Crystal violet.

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Authors: F. Sokhanvar, A. B. Khoshnevis

Abstract:

In the present work steady inviscid hypersonic flows are calculated by approximate Method. Maslens' inverse method is the chosen approximate method. For the inverse problem, parabolic shock shape is chosen for the two-dimensional flow, and the body shape and flow field are calculated using Maslen's method. For the axisymmetric inverse problem paraboloidal shock is chosen and the surface distribution of pressure is obtained.

Keywords: Hypersonic flow, Inverse problem method

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Authors: W. M. Wan Muhamad, E. Sujatmika, M.R. Idris, S.A. Syed Ahmad

Abstract:

A finite element analysis (FEA) computer software HyperWorks is utilized in re-designing an automotive component to reduce its mass. Reduction of components mass contributes towards environmental sustainability by saving world-s valuable metal resources and by reducing carbon emission through improved overall vehicle fuel efficiency. A shape optimization analysis was performed on a rear spindle component. Pre-processing and solving procedures were performed using HyperMesh and RADIOSS respectively. Shape variables were defined using HyperMorph. Then optimization solver OptiStruct was utilized with fatigue life set as a design constraint. Since Stress-Number of Cycle (S-N) theory deals with uni-axial stress, the Signed von Misses stress on the component was used for looking up damage on S-N curve, and Gerber criterion for mean stress corrections. The optimization analysis resulted in mass reduction of 24% of the original mass. The study proved that the adopted approach has high potential use for environmental sustainability.

Keywords: Environmental Sustainability, Shape Optimization, Fatigue, Rear Spindle.

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Authors: Amar Partap Singh Pharwaha, Shweta Rani

Abstract:

To develop a reliable and cost effective communication platform for the telemedicine applications, novel antenna design has been presented using bacterial foraging optimization (BFO) technique. The proposed antenna geometry is achieved by etching a modified Koch curve fractal shape at the edges and a square shape slot at the center of the radiating element of a patch antenna. It has been found that the new antenna has achieved 43.79% size reduction and better resonating characteristic than the original patch. Representative results for both simulations and numerical validations are reported in order to assess the effectiveness of the developed methodology.

Keywords: BFO, electrical permittivity, fractals, Koch curve.

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Authors: A. Shafaghat, H. Khabbaz, S. Moravej, Ah. Shafaghat

Abstract:

The bearing capacity of closely spaced shallow footings alters with their spacing and the shape of footing. In this study, the bearing capacity and settlement of two adjacent footings constructed on a sand layer are investigated. The effect of different footing shapes including square, circular, ring and strip on sandy soil is captured in the calculations. The investigations are carried out numerically using PLAXIS-3D software and analytically employing conventional settlement equations. For this purpose, foundations are modelled in the program with practical dimensions and various spacing ratios ranging from 1 to 5. The spacing ratio is defined as the centre-to-centre distance to the width of foundations (S/B). Overall, 24 models are analyzed; and the results are compared and discussed in detail. It can be concluded that the presence of adjacent foundation leads to the reduction in bearing capacity for round shape footings while it can increase the bearing capacity of rectangular footings in some specific distances.

Keywords: Bearing capacity, finite element analysis, loose sand, settlement equations, shallow foundation.

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Authors: Daniel Amariei, Calin O. Miclosina, Ion Vela, Marius Tufoi, Cornel Mituletu

Abstract:

Even it has been recognized that Shape Memory Alloys (SMA) have a significant potential for deployment actuators, the number of applications of SMA-based actuators to the present day is still quite small, due to the need of deep understanding of the thermo-mechanical behavior of SMA, causing an important need for a mathematical model able to describe all thermo-mechanical properties of SMA by relatively simple final set of constitutive equations. SMAs offer attractive potentials such as: reversible strains of several percent, generation of high recovery stresses and high power / weight ratios. The paper tries to provide an overview of the shape memory functions and a presentation of the designed and developed temperature control system used for a gripper actuated by two pairs of differential SMA active springs. An experimental setup was established, using electrical energy for actuator-s springs heating process. As for holding the temperature of the SMA springs at certain level for a long time was developed a control system in order to avoid the active elements overheating.

Keywords: active element, actuator, model, Nitinol, prehension

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Authors: Oh-Yeol Kwon, Sung-Il Chien

Abstract:

A conventional image posterization method occasionally fails to preserve the shape and color of objects due to the uneffective color reduction. This paper proposes a new image posterizartion method by using modified color quantization for preserving the shape and color of objects and color contrast enhancement for improving lightness contrast and saturation. Experiment results show that our proposed method can provide visually more satisfactory posterization result than that of the conventional method.

Keywords: Color contrast enhancement, color quantization, color segmentation, image posterization

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Authors: Reza Yousefian, Michael A. Kia, Mehrdad Hosseini Zadeh

Abstract:

The blood ducts must be occluded to avoid loss of blood from vessels in laparoscopic surgeries. This paper presents a locking mechanism to be used in a ligation laparoscopic procedure (LigLAP I), as an alternative solution for a stapling procedure. Currently, stapling devices are being used to occlude vessels. Using these devices may result in some problems, including injury of bile duct, taking up a great deal of space behind the vessel, and bile leak. In this new procedure, a two-layer suture occludes a vessel. A locking mechanism is also required to hold the suture. Since there is a limited space at the device tip, a Shape Memory Alloy (SMA) actuator is used in this mechanism. Suitability for cleanroom applications, small size, and silent performance are among the advantages of SMA actuators in biomedical applications. An experimental study is conducted to examine the function of the locking mechanism. To set up the experiment, a prototype of a locking mechanism is built using nitinol, which is a nickel-titanium shape memory alloy. The locking mechanism successfully locks a polymer suture for all runs of the experiment. In addition, the effects of various surface materials on the applied pulling forces are studied. Various materials are mounted at the mechanism tip to compare the maximum pulling forces applied to the suture for each material. The results show that the various surface materials on the device tip provide large differences in the applied pulling forces.

Keywords: Laparoscopic surgery, ligation process, locking mechanism, Shape Memory Alloy (SMA) actuator.

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Authors: N. Azadi-Tinat, H. Oraizi

Abstract:

Design of compact UWB multilayered microstrip filter with E-shape resonator is presented, which provides wide stopband up to 20 GHz and arbitrary impedance matching. The design procedure is developed based on the method of least squares and theory of N-coupled transmission lines. The dimensions of designed filter are about 11 mm × 11 mm and the three E-shape resonators are placed among four dielectric layers. The average insertion loss in the passband is less than 1 dB and in the stopband is about 30 dB up to 20 GHz. Its group delay in the UWB region is about 0.5 ns. The performance of the optimized filter design perfectly agrees with the microwave simulation softwares.

Keywords: Ultra-wideband, method of least square, multilayer microstrip filter, n-coupled transmission lines.

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Authors: M. Areekijseree, W. Pongsawat, M. Pumipaiboon, C. Thepsithar, S. Sengsai, T. Chuen-Im

Abstract:

Morphological interaction of porcine cumulus-oocyte complexes (pCOCs) was investigated on in vitro condition using electron microscope (SEM and TEM). The totals of 1,923 oocytes were round in shape, surrounded by Zona pellucida with layer of cumulus cells ranging between 59.29-202.14 μm in size. They were classified into intact-, multi-, partial cumulus cell layer oocyte, and completely denuded oocyte, at the percentage composition of 22.80% 32.70%, 18.60%, and 25.90 % respectively. The pCOCs classified as intact- and multi cumulus cell layer oocytes were further culturing at 37°C with 5% CO2, 95% air atmosphere and high humidity for 44 h in M199 with Earle’s salts supplemented with 10% HTFCS, 2.2 mg/mL NaHCO3, 1 M Hepes, 0.25 mM pyruvate, 15 μg/mL porcine follicle-stimulating hormone, 1 μg/mL LH, 1μg/mL estradiol with ethanol, and 50 μg/mL gentamycin sulfate. On electron microscope study, cumulus cells were found to stick their processes to secrete substance from the sac-shape end into Zona pellucida of the oocyte and also communicated with the neighboring cells through their microvilli on the beginning of incubation period. It is believed that the cumulus cells communicate with the oocyte by inserting the microvilli through this gap and embedded in the oocyte cytoplasm before secreting substance, through the sac-shape end of the microvilli, to inhibit primary oocyte development at the prophase I. Morphological changes of the complexes were observed after culturing for 24-44 h. One hundred percentages of the cumulus layers were expanded and cumulus cells were peeling off from the oocyte surface. In addition, the round-shape cumulus cells transformed themselves into either an elongate shape or a columnar shape, and no communication between cumulus neighboring cells. After 44 h of incubation time, diameter of oocytes surrounded by cumulus cells was larger than 0 h incubation. The effect of hormones in culture medium is exerted by their receptors present in porcine oocyte. It is likely that all morphological changes of the complexes after hormone treatment were to allow maturation of the oocyte. This study demonstrated that the association of hormones in M199 could promote porcine follicle activation in 44 h in vitro condition. This culture system should be useful for studying the regulation of early follicular growth and development, especially because these follicles represent a large source of oocytes that could be used in vitro for cell technology.

Keywords: Cumulus cells, electron microscopy (SEM and TEM), in vitro, porcine oocyte.

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Authors: H. B. Tibar, Z. Y. Jiang

Abstract:

In this study, the influence of rolling process parameters such as the work roll cross angle and work roll shifting value on the strip shape and profile of aluminum have been investigated under dry conditions at a speed ratio of 1.3 using Hille 100 experimental mill. The strip profile was found to improve significantly with increase in work roll cross angle from 0^o to 1^o, with an associated decrease in rolling force. The effect of roll shifting (from 0 to 8mm) was not as significant as the roll cross angle. However, an increase in work roll shifting value achieved a similar decrease in rolling force as that of work roll cross angle. The effect of work roll shifting was also found to be maximum at an optimum roll speed of 0.0986 m/s for the desired thickness. Of all these parameters, the most significant effect of the strip shape profile was observed with variation of work roll cross angle. However, the rolling force can be a significantly reduced by either increasing the the work roll cross angle or work roll shifting.

Keywords: Rolling speed ratio, strip shape, work roll cross angle, work roll shifting.

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