Search results for: response surface analysis
33586 Synthesis, Characterization and Coating of the Zinc Oxide Nanoparticles on Cotton Fabric by Mechanical Thermo-Fixation Techniques to Impart Antimicrobial Activity
Authors: Imana Shahrin Tania, Mohammad Ali
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The present study reports the synthesis, characterization and application of nano-sized zinc-oxide (ZnO) particles on a cotton fabric surface. The aim of the investigations is to impart the antimicrobial activity on textile cloth. Nanoparticle is synthesized by wet chemical method from zinc sulphate and sodium hydroxide. SEM (scanning electron micrograph) images are taken to demonstrate the surface morphology of nanoparticles. XRD analysis is done to determine the crystal size of the nanoparticle. With the conformation of nanoformation, the cotton woven fabric is treated with ZnO nanoparticle by mechanical thermo-fixation (pad-dry-cure) technique. To increase the wash durability of nano treated fabric, an acrylic binder is used as a fixing agent. The treated fabric shows up to 90% bacterial reduction for S. aureus (Staphylococcus aureus) and 87% for E. coli (Escherichia coli) which is appreciable for bacteria protective clothing.Keywords: nanoparticle, zinc oxide, cotton fabric, antibacterial activity, binder
Procedia PDF Downloads 13233585 Impact of Landuse Change on Surface Temperature in Ibadan, Nigeria
Authors: Abegunde Linda, Adedeji Oluwatola
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It has become increasingly evident that large developments influence the climate within the immediate region and there are concerns that rising temperatures over developed areas could have negative impact and increase living discomfort within city boundaries. Temperature trends in Ibadan city have received minor attention, yet the area has experienced heavy urban expansion between 1972 and 2014. This research aims at examining the impact of landuse change on temperature knowing that the built environment absorbs and stores solar energy, the temperature in cities can be several degrees higher than in adjacent rural areas. This is known as the urban heat island (UHI) effect. The Landsat imagery were used to examine the landuse change for a time period of 42years (1972-2014) and Land surface temperature (LST) was obtained by converting the thermal band to a surface temperature map and zonal statistic analyses was further used to examine the relationship between landuse and temperature emission. The results showed that the settlement area increased by 200km2 while the area covered by vegetation also reduced to about 42.6% during the study period. The spatial and temporal trends of temperature are related to the gradual change in urban landcover and the settlement area has the highest emission of land surface temperature. This research provides useful insight into the temporal behavior of the Ibadan city.Keywords: landuse, LST, remote sensing, UHI
Procedia PDF Downloads 27433584 Seismic Behavior of Three-Dimensional Steel Buildings with Post-Tensioned Connections
Authors: Manuel E. Soto-López, Israel Gaxiola-Avendaño, Alfredo Reyes-Salazar, Eden Bojórquez, Sonia E. Ruiz
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The seismic responses of steel buildings with semi-rigid post-tensioned connections (PC) are estimated and compared with those of steel buildings with typical rigid (welded) connections (RC). The comparison is made in terms of global and local response parameters. The results indicate that the seismic responses in terms of interstory shears, roof displacements, axial load and bending moments are smaller for the buildings with PC connection. The difference is larger for global than for local parameters, which in turn varies from one column location to another. The reason for this improved behavior is that the buildings with PC dissipate more hysteretic energy than those with RC. In addition, unlike the case of buildings with WC, for the PC structures the hysteretic energy is mostly dissipated at the connections, which implies that structural damage in beams and columns is not significant. According to this results, steel buildings with PC are a viable option in highseismicity areas because of their smaller response and self-centering connection capacity as well as the fact that brittle failure is avoided.Keywords: inter-story drift, nonlinear time-history analysis, post-tensioned connections, steel buildings
Procedia PDF Downloads 49933583 Real-Time Optimisation and Minimal Energy Use for Water and Environment Efficient Irrigation
Authors: Kanya L. Khatri, Ashfaque A. Memon, Rod J. Smith, Shamas Bilal
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The viability and sustainability of crop production is currently threatened by increasing water scarcity. Water scarcity problems can be addressed through improved water productivity and the options usually presumed in this context are efficient water use and conversion of surface irrigation to pressurized systems. By replacing furrow irrigation with drip or centre pivot systems, the water efficiency can be improved by up to 30 to 45%. However, the installation and application of pumps and pipes, and the associated fuels needed for these alternatives increase energy consumption and cause significant greenhouse gas emissions. Hence, a balance between the improvement in water use and the potential increase in energy consumption is required keeping in view adverse impact of increased carbon emissions on the environment. When surface water is used, pressurized systems increase energy consumption substantially, by between 65% to 75%, and produce greenhouse gas emissions around 1.75 times higher than that of gravity based irrigation. With gravity based surface irrigation methods the energy consumption is assumed to be negligible. This study has shown that a novel real-time infiltration model REIP has enabled implementation of real-time optimization and control of surface irrigation and surface irrigation with real-time optimization has potential to bring significant improvements in irrigation performance along with substantial water savings of 2.92 ML/ha which is almost equivalent to that given by pressurized systems. Thus real-time optimization and control offers a modern, environment friendly and water efficient system with close to zero increase in energy consumption and minimal greenhouse gas emissions.Keywords: pressurised irrigation, carbon emissions, real-time, environmentally-friendly, REIP
Procedia PDF Downloads 50333582 Approximating a Funicular Shape with a Translational Surface, Example of a Glass Canopy
Authors: Raphaël Menard, Etienne Fayette, Paul Azzopardi
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This paper presents the method to generate the geometry of an actual glass canopy project in Rennes, France, by architect Bruno Gaudin, with aim to achieve the best structural efficiency possible using only quadrangle meshing. The paper includes equation of the translational surface generated, the level of accuracy in approximating the funicular shape and the method of constructive implementation.Keywords: funicular shape, glass canopy, glass panels, lowered arches, mathematics, penalization, shell structure
Procedia PDF Downloads 55333581 A Solution to Analyze the Geosynthetic Reinforced Piled Embankments Considering Pile-Soil Interaction
Authors: Feicheng Liu, Weiming Liao, Jianjing Zhang
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A pile-supported embankment with geosynthetic-reinforced mat (PSGR embankment) has been considered as an effective solution to reduce the total and differential settlement of the embankment constructed over soft soil. In this paper, a new simplified method proposed firstly incorporates the load transfer between piles and surrounding soil and the settlement of pile, and also considers arching effect in embankment fill, membrane effect of geosynthetic reinforcement, and subsoil resistance, to evaluate the behavior of PSGR embankment. Subsoil settlement is assumed to consist of two parts:(1) the settlement of subsoil surface between piles equivalent to that of pile caps assuming the geosynthetic reinforcement without deformation yet; (2) the subsoil subsiding along with the geosynthetic deforming, and the deflected geosynthetic being considered as centenary. The force equilibrium, including loads acting on the upper surface of geosynthetic, subsoil resistance, as well as the stress-strain relationship of the geosynthetic reinforcement at the edge of pile cap, is established, thus the expression of subsoil resistance is deduced, and subsequently the tension of geosynthetic and stress concentration ratio between piles can be calculated. The proposed method is validated through observed data from three field tests and also compared with other eight analytical solutions available in the literature. In addition, a sensitive analysis is provided to demonstrate the influence of with/without considering pile-soil interaction for evaluating the performance of PSGR embankment.Keywords: pile-supported embankment, geosynthetic, analytical solution, soil arching effect, the settlement of pile, sensitive analysis
Procedia PDF Downloads 15733580 Viability of Irrigation Water Conservation Practices in the Low Desert of California
Authors: Ali Montazar
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California and the Colorado River Basin are facing increasing uncertainty concerning water supplies. The Colorado River is the main source of irrigation water in the low desert of California. Currently, due to an increasing water-use competition and long-term drought at the Colorado River Basin, efficient use of irrigation water is one of the highest conservation priorities in the region. This study aims to present some of current irrigation technologies and management approaches in the low desert and assess the viability and potential of these water management practices. The results of several field experiments are used to assess five water conservation practices of sub-surface drip irrigation, automated surface irrigation, sprinkler irrigation, tail-water recovery system, and deficit irrigation strategy. The preliminary results of several ongoing studies at commercial fields are presented, particularly researches in alfalfa, sugar beets, kliengrass, sunflower, and spinach fields. The findings indicate that all these practices have significant potential to conserve water (an average of 1 ac-ft/ac) and enhance the efficiency of water use (15-25%). Further work is needed to better understand the feasibility of each of these applications and to help maintain profitable and sustainable agricultural production system in the low desert as water and labor costs, and environmental issues increase.Keywords: automated surface irrigation, deficit irrigation, low desert of California, sprinkler irrigation, sub-surface drip irrigation, tail-water recovery system
Procedia PDF Downloads 15833579 Assessment of the Photovoltaic and Solar Thermal Potential Installation Area on Residential Buildings: Case Study of Amman, Jordan
Authors: Jenan Abu Qadourah
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The suitable surface areas for the ST and PV installation are determined based on incident solar irradiation on different surfaces, shading analysis and suitable architectural area for integration considering limitations due to the constructions, available surfaces area and use of the available surfaces for other purposes. The incident solar radiation on the building surfaces and the building solar exposure analysis of the location of Amman, Jordan, is performed with Autodesk Ecotect analysis 2011 simulation software. The building model geometry within the typical urban context is created in “SketchUp,” which is then imported into Ecotect. The hourly climatic data of Amman, Jordan selected are the same ones used for the building simulation in IDA ICE and Polysun simulation software.Keywords: photovoltaic, solar thermal, solar incident, simulation, building façade, solar potential
Procedia PDF Downloads 14033578 Thermoluminescent Response of Nanocrystalline BaSO4:Eu to 85 MeV Carbon Beams
Authors: Shaila Bahl, S. P. Lochab, Pratik Kumar
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Nanotechnology and nanomaterials have attracted researchers from different fields, especially from the field of luminescence. Recent studies on various luminescent nanomaterials have shown their relevance in dosimetry of ionizing radiations for the measurements of high doses using the Thermoluminescence (TL) technique, where the conventional microcrystalline phosphors saturate. Ion beams have been used for diagnostic and therapeutic purposes due to their favorable profile of dose deposition at the end of the range known as the Bragg peak. While dealing with human beings, doses from these beams need to be measured with great precision and accuracy. Henceforth detailed investigations of suitable thermoluminescent dosimeters (TLD) for dose verification in ion beam irradiation are required. This paper investigates the TL response of nanocrystalline BaSO4 doped with Eu to 85 MeV carbon beam. The synthesis was done using Co-precipitation technique by mixing Barium chloride and ammonium sulphate solutions. To investigate the crystallinity and particle size, analytical techniques such as X-ray diffraction (XRD) and Transmission electron microscopy (TEM) were used which revealed the average particle sizes to 45 nm with orthorhombic structure. Samples in pellet form were irradiated by 85 MeV carbon beam in the fluence range of 1X1010-5X1013. TL glow curves of the irradiated samples show two prominent glow peaks at around 460 K and 495 K. The TL response is linear up to 1X1013 fluence after which saturation was observed. The wider linear TL response of nanocrystalline BaSO4: Eu and low fading make it a superior candidate as a dosimeter to be used for detecting the doses of carbon beam.Keywords: radiation, dosimetry, carbon ions, thermoluminescence
Procedia PDF Downloads 28933577 Sero-Prevalence of Hepatitis B Surface Antigen and Associated Factors among Pregnant Mothers Attending Antenatal Care Service, Mekelle, Ethiopia: Evidence from Institutional Based Quantitative Cross-Sectional Study
Authors: Semaw A., Awet H., Yohannes M.
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Background: Hepatitis B Virus (HBV) is a major global public health problem. Individuals living in Sub-Sahara Africa have 60% lifetime risk of acquiring HBV infection. Evidences showed that 80-90% of those born from infected mothers developed chronic HBV. Perinatal HBV transmission is a major determinant of HBV carrier status, its chronic squeal and maintains HBV transmission across generations. Method: Institution based cross-sectional study was conducted among 406 pregnant mothers attending Antenatal clinics at Mekelle and Ayder referral hospital from January 30 to April 1/2014. Epidata version 3.1 was used for data entry and SPSS version 21 statistical software was used for data cleaning, management and finally determine associated factors of hepatitis B surface antigen adjusting important confounders using multivariable logistic regression analysis at 5% level of significance. Result: The overall prevalence of hepatitis B surface antigen among pregnant women was 33 (8.1%). The socio-demographic characteristic of the study population showed that there is high positivity among secondary school 189 (46.6%). In the multivariable logistic regression analysis, history of a contact with individuals who had history of hepatitis B infection or jaundice and lifetime number of multiple sexual partners were found to be significantly associated with HBsAg positivity at AOR = 3.73 95%C.I (1.373-10.182) and AOR = 2.57 95%C.I (1.173-5.654), respectively. Moreover, Human Immunodeficiency Virus (HIV) and HBV confection rate was found 3.6%. Conclusion: This study has shown that HBV prevalence in pregnant women is highly prevalent (8.1%) in the study area. Contact with individuals who had a history of hepatitis or have jaundice and report of multiple lifetime sexual partnership were associated with hepatitis B infection. Education about HBV transmission and prevention as well as screening all pregnant mothers shall be sought to reduce the serious public health crisis of HBV.Keywords: HBsAg, hepatitis B, pregnant women, prevalence
Procedia PDF Downloads 34033576 Failure Analysis of a Medium Duty Vehicle Leaf Spring
Authors: Gül Çevik
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This paper summarizes the work conducted to assess the root cause of the failure of a medium commercial vehicle leaf spring failed in service. Macro- and micro-fractographic analyses by scanning electron microscope as well as material verification tests were conducted in order to understand the failure mechanisms and root cause of the failure. Findings from the fractographic analyses indicated that failure mechanism is fatigue. Crack initiation was identified to have occurred from a point on the top surface near to the front face and to the left side. Two other crack initiation points were also observed, however, these cracks did not propagate. The propagation mode of the fatigue crack revealed that the cyclic loads resulting in crack initiation and propagation were unidirectional bending. Fractographic analyses have also showed that the root cause of the fatigue crack initiation and propagation was loading the part above design stress. Material properties of the part were also verified by chemical composition analysis, microstructural analysis by optical microscopy and hardness tests.Keywords: leaf spring, failure analysis, fatigue, fractography
Procedia PDF Downloads 13333575 Contact Temperature of Sliding Surfaces in AISI 316 Austenitic Stainless Steel During PIN on Disk Dry Wear Testing
Authors: Dler Abdullah Ahmed, Zozan Ahmed Mohammed
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This study looked into contact surface temperature during a pin-on-disk test. Friction and wear between sliding surfaces raised the temperature differential between the contact surface and ambient temperatures Tdiff. Tdiff was significantly influenced by wear test variables. Tdiff rose with the increase of sliding speed and applied load while dropped with the increase in ambient temperature. The highest Tdiff was 289°C during the tests at room temperature and 2.5 m/s sliding speed, while the minimum was only 24 °C during the tests at 400°C and 0.5 m/s. However, the maximum contact temperature Tmax was found during tests conducted at high ambient temperatures. The Tmax was estimated based on the theoretical equation. The comparison of experimental and theoretical Tmax data revealed good agreement.Keywords: pin on disk test, contact temperature, wear, sliding surface, friction, ambient temperature
Procedia PDF Downloads 8233574 Synergetic Effects of Water and Sulfur Dioxide Treatments on Wear of Soda Lime Silicate Glass
Authors: Qian Qiao, Tongjin Xiao, Hongtu He, Jiaxin Yu
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This study is focused on the synergetic effects of water and sulfur dioxide treatments (SO₂ treatments) on the mechanochemical wear of SLS glass. It is found that the wear behavior of SLS glass in humid air is very sensitive to the water and SO₂ treatment environments based on the wear test using a ball-on-flat reciprocation tribometer. When SLS glass is treated with SO₂-without, the presence of water, the wear resistance of SLS glass in humid air becomes significantly higher compared to the pristine glass. However, when SLS glass is treated with SO₂ with the presence of water, the wear resistance of SLS glass decreases remarkably with increasing in the relative humidity (RH) from 0% to 90%. Further analyses indicate that when sodium ions are leached out of SLS glass surface via the water and SO₂ treatments, the mechanochemical properties of SLS glass surface become different depending on the RH. At lower humidity, the nano hardness of the Na⁺-leached surface is higher, and it can contribute to the enhanced wear resistance of SLS glass. In contrast, at higher humidity conditions, the SLS glass surface is more hydrophilic, and substantial wear debris can be found inside the wear track of SLS glass. Those phenomena suggest that adhesive wear and abrasive wear dominate the wear mechanism of SLS glass in humid air, causing the decreased wear resistance of SLS glass with increasing the RH. These results may not only provide a deep understanding of the wear mechanism of SLS glass but also helpful for operation process of functional and engineering glasses.Keywords: soda lime silicate glass, wear, water, SO₂
Procedia PDF Downloads 17633573 Effect of Liquid Additive on Dry Grinding for Desired Surface Structure of CaO Catalyst
Authors: Wiyanti Fransisca Simanullang, Shinya Yamanaka
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Grinding method was used to control the active site and to improve the specific surface area (SSA) of calcium oxide (CaO) derived from scallop shell as a sustainable resource. The dry grinding of CaO with acetone and tertiary butanol as a liquid additive was carried out using a planetary ball mill with a laboratory scale. The experiments were operated by stepwise addition with time variations to determine the grinding limit. The active site of CaO was measured by X-Ray Diffraction and FT-IR. The SSA variations of products with grinding time were measured by BET method. The morphology structure of CaO was observed by SEM. The use of liquid additive was effective for increasing the SSA and controlling the active site of CaO. SSA of CaO was increased in proportion to the amount of the liquid additive and the grinding time. The performance of CaO as a solid base catalyst for biodiesel production was tested in the transesterification reaction of used cooking oil to produce fatty acid methyl ester (FAME).Keywords: active site, calcium oxide, grinding, specific surface area
Procedia PDF Downloads 28833572 Synthesis and Characterization of Non-Aqueous Electrodeposited ZnSe Thin Film
Authors: S. R. Kumar, Shashikant Rajpal
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A nanocrystalline thin film of ZnSe was successfully electrodeposited on copper substrate using a non-aqueous solution and subsequently annealed in air at 400°C. XRD analysis indicates the polycrystalline deposit of (111) plane in both the cases. The sharpness of the peak increases due to annealing of the film and average grain size increases to 20 nm to 27nm. SEM photograph indicate that grains are uniform and densely distributed over the surface. Due to annealing the average grain size increased by 20%. The EDS spectroscopy shows the ratio of Zn & Se is 1.1 in case of annealed film. AFM analysis indicates the average roughness of the film reduces from 181nm to 165nm due to annealing of the film. The bandgap also decreases from 2.71eV to 2.62eV.Keywords: electrodeposition, non-aqueous medium, SEM, XRD
Procedia PDF Downloads 48633571 Research on Static and Dynamic Behavior of New Combination of Aluminum Honeycomb Panel and Rod Single-Layer Latticed Shell
Authors: Xu Chen, Zhao Caiqi
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In addition to the advantages of light weight, resistant corrosion and ease of processing, aluminum is also applied to the long-span spatial structures. However, the elastic modulus of aluminum is lower than that of the steel. This paper combines the high performance aluminum honeycomb panel with the aluminum latticed shell, forming a new panel-and-rod composite shell structure. Through comparative analysis between the static and dynamic performance, the conclusion that the structure of composite shell is noticeably superior to the structure combined before.Keywords: combination of aluminum honeycomb panel, rod latticed shell, dynamic performence, response spectrum analysis, seismic properties
Procedia PDF Downloads 47333570 SEM Detection of Folate Receptor in a Murine Breast Cancer Model Using Secondary Antibody-Conjugated, Gold-Coated Magnetite Nanoparticles
Authors: Yasser A. Ahmed, Juleen M Dickson, Evan S. Krystofiak, Julie A. Oliver
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Cancer cells urgently need folate to support their rapid division. Folate receptors (FR) are over-expressed on a wide range of tumor cells, including breast cancer cells. FR are distributed over the entire surface of cancer cells, but are polarized to the apical surface of normal cells. Targeting of cancer cells using specific surface molecules such as folate receptors may be one of the strategies used to kill cancer cells without hurting the neighing normal cells. The aim of the current study was to try a method of SEM detecting FR in a murine breast cancer cell model (4T1 cells) using secondary antibody conjugated to gold or gold-coated magnetite nanoparticles. 4T1 cells were suspended in RPMI medium witth FR antibody and incubated with secondary antibody for fluorescence microscopy. The cells were cultured on 30mm Thermanox coverslips for 18 hours, labeled with FR antibody then incubated with secondary antibody conjugated to gold or gold-coated magnetite nanoparticles and processed to scanning electron microscopy (SEM) analysis. The fluorescence microscopy study showed strong punctate FR expression on 4T1 cell membrane. With SEM, the labeling with gold or gold-coated magnetite conjugates showed a similar pattern. Specific labeling occurred in nanoparticle clusters, which are clearly visualized in backscattered electron images. The 4T1 tumor cell model may be useful for the development of FR-targeted tumor therapy using gold-coated magnetite nano-particles.Keywords: cancer cell, nanoparticles, cell culture, SEM
Procedia PDF Downloads 73533569 A Numerical Method to Evaluate the Elastoplastic Material Properties of Fiber Reinforced Composite
Authors: M. Palizvan, M. H. Sadr, M. T. Abadi
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The representative volume element (RVE) plays a central role in the mechanics of random heterogeneous materials with a view to predicting their effective properties. In this paper, a computational homogenization methodology, developed to determine effective linear elastic properties of composite materials, is extended to predict the effective nonlinear elastoplastic response of long fiber reinforced composite. Finite element simulations of volumes of different sizes and fiber volume fractures are performed for calculation of the overall response RVE. The dependencies of the overall stress-strain curves on the number of fibers inside the RVE are studied in the 2D cases. Volume averaged stress-strain responses are generated from RVEs and compared with the finite element calculations available in the literature at moderate and high fiber volume fractions. For these materials, the existence of an RVE is demonstrated for the sizes of RVE corresponding to 10–100 times the diameter of the fibers. In addition, the response of small size RVE is found anisotropic, whereas the average of all large ones leads to recover the isotropic material properties.Keywords: homogenization, periodic boundary condition, elastoplastic properties, RVE
Procedia PDF Downloads 15333568 Influence of Surface Wettability on Imbibition Dynamics of Protein Solution in Microwells
Authors: Himani Sharma, Amit Agrawal
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Stability of the Cassie and Wenzel wetting states depends on intrinsic contact angle and geometric features on a surface that was exploited in capturing biofluids in microwells. However, the mechanism of imbibition of biofluids in the microwells is not well implied in terms of wettability of a substrate. In this work, we experimentally demonstrated filling dynamics in hydrophilic and hydrophobic microwells by protein solutions. Towards this, we utilized lotus leaf as a mold to fabricate microwells on a Polydimethylsiloxane (PDMS) surface. Lotus leaf containing micrometer-sized blunt-conical shaped pillars with a height of 8-15 µm and diameter of 3-8 µm were transferred on to PDMS. Furthermore, PDMS surface was treated with oxygen plasma to render the hydrophilic nature. A 10µL droplets containing fluorescein isothiocyanate (FITC) - labelled bovine serum albumin (BSA) were rested on both hydrophobic (θa = 108o, where θa is the apparent contact angle) and hydrophilic (θa = 60o) PDMS surfaces. A time-dependent fluorescence microscopy was conducted on these modified PDMS surfaces by recording the fluorescent intensity over a 5 minute period. It was observed that, initially (at t=1 min) FITC-BSA was accumulated on the periphery of both hydrophilic and hydrophobic microwells due to incomplete penetration of liquid-gas meniscus. This deposition of FITC-BSA on periphery of microwell was not changed with time for hydrophobic surfaces, whereas, a complete filling was occurred in hydrophilic microwells (at t=5 mins). This attributes to a gradual movement of three-phase contact line along the vertical surface of the hydrophilic microwells as compared to stable pinning in the hydrophobic microwells as confirmed by Surface Evolver simulations. In addition, if the cavities are presented on hydrophobic surfaces, air bubbles will be trapped inside the cavities once the aqueous solution is placed over these surfaces, resulting in the Cassie-Baxter wetting state. This condition hinders trapping of proteins inside the microwells. Thus, it is necessary to impart hydrophilicity to the microwell surfaces so as to induce the Wenzel state, such that, an entire solution will be fully in contact with the walls of microwells. Imbibition of microwells by protein solutions was analyzed in terms fluorescent intensity versus time. The present work underlines the importance of geometry of microwells and surface wettability of substrate in wetting and effective capturing of solid sub-phases in biofluids.Keywords: BSA, microwells, surface evolver, wettability
Procedia PDF Downloads 19833567 Fluoride-Induced Stress and Its Association with Bone Developmental Pathway in Osteosarcoma Cells
Authors: Deepa Gandhi, Pravin K. Naoghare, Amit Bafana, Krishnamurthi Kannan, Saravanadevi Sivanesana
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Oxidative stress is known to depreciate normal functioning of osteoblast cells. Present study reports oxidative/inflammatory signatures in fluoride exposed human osteosarcoma (HOS) cells and its possible association with the genes involved in bone developmental pathway. Microarray analysis was performed to understand the possible molecular mechanisms of stress-mediated bone lose in HOS cells. Cells were chronically exposed with sub-lethal concentration of fluoride. Global gene expression is profiling revealed 34 up regulated and 2598 down-regulated genes, which were associated with several biological processes including bone development, osteoblast differentiation, stress response, inflammatory response, apoptosis, regulation of cell proliferation. Microarray data were further validated through qRT-PCR and western blot analyses using key representative genes. Based on these findings, it can be proposed that chronic exposure of fluoride may impair bone development via oxidative and inflammatory stress. The present finding also provides important biological clues, which will be helpful for the development of therapeutic targets against diseases related bone.Keywords: bone, HOS cells, microarray, stress
Procedia PDF Downloads 37733566 Modeling and Optimizing of Sinker Electric Discharge Machine Process Parameters on AISI 4140 Alloy Steel by Central Composite Rotatable Design Method
Authors: J. Satya Eswari, J. Sekhar Babub, Meena Murmu, Govardhan Bhat
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Electrical Discharge Machining (EDM) is an unconventional manufacturing process based on removal of material from a part by means of a series of repeated electrical sparks created by electric pulse generators at short intervals between a electrode tool and the part to be machined emmersed in dielectric fluid. In this paper, a study will be performed on the influence of the factors of peak current, pulse on time, interval time and power supply voltage. The output responses measured were material removal rate (MRR) and surface roughness. Finally, the parameters were optimized for maximum MRR with the desired surface roughness. RSM involves establishing mathematical relations between the design variables and the resulting responses and optimizing the process conditions. RSM is not free from problems when it is applied to multi-factor and multi-response situations. Design of experiments (DOE) technique to select the optimum machining conditions for machining AISI 4140 using EDM. The purpose of this paper is to determine the optimal factors of the electro-discharge machining (EDM) process investigate feasibility of design of experiment techniques. The work pieces used were rectangular plates of AISI 4140 grade steel alloy. The study of optimized settings of key machining factors like pulse on time, gap voltage, flushing pressure, input current and duty cycle on the material removal, surface roughness is been carried out using central composite design. The objective is to maximize the Material removal rate (MRR). Central composite design data is used to develop second order polynomial models with interaction terms. The insignificant coefficients’ are eliminated with these models by using student t test and F test for the goodness of fit. CCD is first used to establish the determine the optimal factors of the electro-discharge machining (EDM) for maximizing the MRR. The responses are further treated through a objective function to establish the same set of key machining factors to satisfy the optimization problem of the electro-discharge machining (EDM) process. The results demonstrate the better performance of CCD data based RSM for optimizing the electro-discharge machining (EDM) process.Keywords: electric discharge machining (EDM), modeling, optimization, CCRD
Procedia PDF Downloads 34133565 Reliability Analysis of Glass Epoxy Composite Plate under Low Velocity
Authors: Shivdayal Patel, Suhail Ahmad
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Safety assurance and failure prediction of composite material component of an offshore structure due to low velocity impact is essential for associated risk assessment. It is important to incorporate uncertainties associated with material properties and load due to an impact. Likelihood of this hazard causing a chain of failure events plays an important role in risk assessment. The material properties of composites mostly exhibit a scatter due to their in-homogeneity and anisotropic characteristics, brittleness of the matrix and fiber and manufacturing defects. In fact, the probability of occurrence of such a scenario is due to large uncertainties arising in the system. Probabilistic finite element analysis of composite plates due to low-velocity impact is carried out considering uncertainties of material properties and initial impact velocity. Impact-induced damage of composite plate is a probabilistic phenomenon due to a wide range of uncertainties arising in material and loading behavior. A typical failure crack initiates and propagates further into the interface causing de-lamination between dissimilar plies. Since individual crack in the ply is difficult to track. The progressive damage model is implemented in the FE code by a user-defined material subroutine (VUMAT) to overcome these problems. The limit state function is accordingly established while the stresses in the lamina are such that the limit state function (g(x)>0). The Gaussian process response surface method is presently adopted to determine the probability of failure. A comparative study is also carried out for different combination of impactor masses and velocities. The sensitivity based probabilistic design optimization procedure is investigated to achieve better strength and lighter weight of composite structures. Chain of failure events due to different modes of failure is considered to estimate the consequences of failure scenario. Frequencies of occurrence of specific impact hazards yield the expected risk due to economic loss.Keywords: composites, damage propagation, low velocity impact, probability of failure, uncertainty modeling
Procedia PDF Downloads 27933564 An Assessment of Water and Sediment Quality of the Danube River: Polycyclic Aromatic Hydrocarbons and Trace Metals
Authors: A. Szabó Nagy, J. Szabó, I. Vass
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Water and sediment samples from the Danube River and Moson Danube Arm (Hungary) have been collected and analyzed for contamination by 18 polycyclic aromatic hydrocarbons (PAHs) and eight trace metal(loid)s (As, Cu, Pb, Ni, Cr, Cd, Hg and Zn) in the period of 2014-2015. Moreover, the trace metal(loid) concentrations were measured in the Rába and Marcal rivers (parts of the tributary system feeding the Danube). Total PAH contents in water were found to vary from 0.016 to 0.133 µg/L and concentrations in sediments varied in the range of 0.118 mg/kg and 0.283 mg/kg. Source analysis of PAHs using diagnostic concentration ratios indicated that PAHs found in sediments were of pyrolytic origins. The dissolved trace metal and arsenic concentrations were relatively low in the surface waters. However, higher concentrations were detected in the water samples of Rába (Zn, Cu, Ni, Pb) and Marcal (As, Cu, Ni, Pb) compared to the Danube and Moson Danube. The concentrations of trace metals in sediments were higher than those found in water samples.Keywords: surface water, sediment, PAH, trace metal
Procedia PDF Downloads 31533563 The Effect of Surface Modifiers on the Mechanical and Morphological Properties of Waste Silicon Carbide Filled High-Density Polyethylene
Authors: R. Dangtungee, A. Rattanapan, S. Siengchin
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Waste silicon carbide (waste SiC) filled high-density polyethylene (HDPE) with and without surface modifiers were studied. Two types of surface modifiers namely; high-density polyethylene-grafted-maleic anhydride (HDPE-g-MA) and 3-aminopropyltriethoxysilane have been used in this study. The composites were produced using a two roll mill, extruder and shaped in a hydraulic compression molding machine. The mechanical properties of polymer composites such as flexural strength and modulus, impact strength, tensile strength, stiffness and hardness were investigated over a range of compositions. It was found that, flexural strength and modulus, tensile modulus and hardness increased, whereas impact strength and tensile strength decreased with the increasing in filler contents, compared to the neat HDPE. At similar filler content, the effect of both surface modifiers increased flexural modulus, impact strength, tensile strength and stiffness but reduced the flexural strength. Morphological investigation using SEM revealed that the improvement in mechanical properties was due to enhancement of the interfacial adhesion between waste SiC and HDPE.Keywords: high-density polyethylene, HDPE-g-MA, mechanical properties, morphological properties, silicon carbide, waste silicon carbide
Procedia PDF Downloads 36333562 Preparation and Evaluation of Herbal Extracts for Washing of Vegetables and Fruits
Authors: Pareshkumar Umedbhai Patel
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Variety of microbes were isolated from surface of fruit and vegetables to get idea about normal flora of their surface. The process of isolation of microbes involved use of sterilized cotton swabs to wipe the surface of the samples. For isolation of Bacteria, yeast and fungi microbiological media used were nutrient agar medium, GYE agar medium and MRBA agar medium respectively. The microscopical and macroscopical characteristics of all the isolates were studied. Different plants with known antimicrobial activity were selected for obtaining samples for extraction e.g. Ficus (Ficus religosa) stem, Amla (Phyllanthus emblica) fruit, Tulsi (Ocimum tenuiflorum) leaves and Lemon grass (Cymbopogon citratus) oil. Antimicrobial activity of these samples was tested initially against known bacteria followed by study against microbes isolated from surface of vegetables and fruits. During the studies carried out throughout the work, lemongrass oil and Amla extract were found superior. Lemongrass oil and Amla extract respectively inhibited growth of 65% and 42% microbes isolated from fruit and vegetable surfaces. Rest two studied plant extracts showed only 11% of inhibition against the studied isolates. The results of isolate inhibition show the antibacterial effect of lemongrass oil better than the rest of the studied plant extracts.Keywords: herbal extracts, vegetables, fruits, antimicrobial activity
Procedia PDF Downloads 16633561 Predicting the Human Impact of Natural Onset Disasters Using Pattern Recognition Techniques and Rule Based Clustering
Authors: Sara Hasani
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This research focuses on natural sudden onset disasters characterised as ‘occurring with little or no warning and often cause excessive injuries far surpassing the national response capacities’. Based on the panel analysis of the historic record of 4,252 natural onset disasters between 1980 to 2015, a predictive method was developed to predict the human impact of the disaster (fatality, injured, homeless) with less than 3% of errors. The geographical dispersion of the disasters includes every country where the data were available and cross-examined from various humanitarian sources. The records were then filtered into 4252 records of the disasters where the five predictive variables (disaster type, HDI, DRI, population, and population density) were clearly stated. The procedure was designed based on a combination of pattern recognition techniques and rule-based clustering for prediction and discrimination analysis to validate the results further. The result indicates that there is a relationship between the disaster human impact and the five socio-economic characteristics of the affected country mentioned above. As a result, a framework was put forward, which could predict the disaster’s human impact based on their severity rank in the early hours of disaster strike. The predictions in this model were outlined in two worst and best-case scenarios, which respectively inform the lower range and higher range of the prediction. A necessity to develop the predictive framework can be highlighted by noticing that despite the existing research in literature, a framework for predicting the human impact and estimating the needs at the time of the disaster is yet to be developed. This can further be used to allocate the resources at the response phase of the disaster where the data is scarce.Keywords: disaster management, natural disaster, pattern recognition, prediction
Procedia PDF Downloads 15333560 River Analysis System Model for Proposed Weirs at Downstream of Large Dam, Thailand
Authors: S. Chuenchooklin
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This research was conducted in the Lower Ping River Basin downstream of the Bhumibol Dam and the Lower Wang River Basin in Tak Province, Thailand. Most of the tributary streams of the Ping can be considered as ungauged catchments. There are 10- pumping station installation at both river banks of the Ping in Tak Province. Recently, most of them could not fully operate due to the water amount in the river below the level that would be pumping, even though included water from the natural river and released flow from the Bhumibol Dam. The aim of this research was to increase the performance of those pumping stations using weir projects in the Ping. Therefore, the river analysis system model (HEC-RAS) was applied to study the hydraulic behavior of water surface profiles in the Ping River with both cases of existing conditions and proposed weirs during the violent flood in 2011 and severe drought in 2013. Moreover, the hydrologic modeling system (HMS) was applied to simulate lateral streamflow hydrograph from ungauged catchments of the Ping. The results of HEC-RAS model calibration with existing conditions in 2011 showed best trial roughness coefficient for the main channel of 0.026. The simulated water surface levels fitted to observation data with R2 of 0.8175. The model was applied to 3 proposed cascade weirs with 2.35 m in height and found surcharge water level only 0.27 m higher than the existing condition in 2011. Moreover, those weirs could maintain river water levels and increase of those pumping performances during less river flow in 2013.Keywords: HEC-RAS, HMS, pumping stations, cascade weirs
Procedia PDF Downloads 39033559 Quantitative Structure-Activity Relationship Analysis of Binding Affinity of a Series of Anti-Prion Compounds to Human Prion Protein
Authors: Strahinja Kovačević, Sanja Podunavac-Kuzmanović, Lidija Jevrić, Milica Karadžić
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The present study is based on the quantitative structure-activity relationship (QSAR) analysis of eighteen compounds with anti-prion activity. The structures and anti-prion activities (expressed in response units, RU%) of the analyzed compounds are taken from CHEMBL database. In the first step of analysis 85 molecular descriptors were calculated and based on them the hierarchical cluster analysis (HCA) and principal component analysis (PCA) were carried out in order to detect potential significant similarities or dissimilarities among the studied compounds. The calculated molecular descriptors were physicochemical, lipophilicity and ADMET (absorption, distribution, metabolism, excretion and toxicity) descriptors. The first stage of the QSAR analysis was simple linear regression modeling. It resulted in one acceptable model that correlates Henry's law constant with RU% units. The obtained 2D-QSAR model was validated by cross-validation as an internal validation method. The validation procedure confirmed the model’s quality and therefore it can be used for prediction of anti-prion activity. The next stage of the analysis of anti-prion activity will include 3D-QSAR and molecular docking approaches in order to select the most promising compounds in treatment of prion diseases. These results are the part of the project No. 114-451-268/2016-02 financially supported by the Provincial Secretariat for Science and Technological Development of AP Vojvodina.Keywords: anti-prion activity, chemometrics, molecular modeling, QSAR
Procedia PDF Downloads 30433558 Optimal Configuration for Polarimetric Surface Plasmon Resonance Sensors
Authors: Ibrahim Watad, Ibrahim Abdulhalim
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Conventional spectroscopic surface plasmon resonance (SPR) sensors are widely used, both in fundamental research and environmental monitoring as well as healthcare diagnostics. However, they still lack the low limit of detection (LOD) and there still a place for improvement. SPR conventional sensors are based on the detection of a dip in the reflectivity spectrum which is relatively wide. To improve the performance of these sensors, many techniques and methods proposed either to reduce the width of the dip or to increase the sensitivity. Together with that, profiting from the sharp jump in the phase spectrum under SPR, several works suggested the extraction of the phase of the reflected wave. However, existing phase measurement setups are in general more complicated compared to the conventional setups, require more stability and are very sensitive to external vibrations and noises. In this study, a simple polarimetric technique for phase extraction under SPR is presented, followed by a theoretical error analysis and an experimental verification. The advantages of the proposed technique upon existing techniques will be elaborated, together with conclusions regarding the best polarimetric function, and its corresponding optimal metal layer range of thicknesses to use under the conventional Kretschmann-Raether configuration.Keywords: plasmonics, polarimetry, thin films, optical sensors
Procedia PDF Downloads 40433557 A Systamatic Review on Experimental, FEM Analysis and Simulation of Metal Spinning Process
Authors: Amol M. Jadhav, Sharad S. Chudhari, S. S. Khedkar
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This review presents a through survey of research paper work on the experimental analysis, FEM Analysis & simulation of the metal spinning process. In this literature survey all the papers being taken from Elsevier publication and most of the from journal of material processing technology. In a last two decade or so, metal spinning process gradually used as chip less formation for the production of engineering component in a small to medium batch quantities. The review aims to provide include into the experimentation, FEM analysis of various components, simulation of metal spinning process and act as guide for research working on metal spinning processes. The review of existing work has several gaps in current knowledge of metal spinning processes. The evaluation of experiment is thickness strain, the spinning force, the twisting angle, the surface roughness of the conventional & shear metal spinning process; the evaluation of FEM of metal spinning to path definition with sufficient fine mesh to capture behavior of work piece; The evaluation of feed rate of roller, direction of roller,& type of roller stimulated. The metal spinning process has the more flexible to produce a wider range of product shape & to form more challenge material.Keywords: metal spinning, FEM analysis, simulation of metal spinning, mechanical engineering
Procedia PDF Downloads 387