Search results for: Tanya Soni
25 Data Security in a DApp Twitter Alike on Web 3.0 With Blockchain Based Technology
Authors: Vishal Awasthi, Tanya Soni, Vigya Awasthi, Swati Singh, Shivali Verma
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There is a growing demand for a network that grants a high level of data security and confidentiality. For this reason, the semantic web was introduced, which allows data to be shared and reused across applications while safeguarding users privacy and user’s will grab back control of their data. The earlier Web 1.0 and Web 2.0 versions were built on client-server architecture, in which there was the risk of data theft and unconsented sale of user data. A decentralized version, Known as Web 3.0, that is mostly built on blockchain technology was interjected to resolve these issues. The recent research focuses on blockchain technology, deals with privacy, security, transparency, and innovation of decentralized applications (DApps), e.g. a Twitter Clone, Whatsapp clone. In this paper the Twitter Alike built on the Ethereum blockchain will replace traditional techniques with improved latency, throughput, and data ownership. The central principle of this DApp is smart contract implemented using Solidity which is an object- oriented and highlevel language. Consequently, this will provide a better Quality Services, high data security, and integrity for both present and future internet technologies.
Keywords: Blockchain, DApps, Ethereum, Semantic Web, Smart Contract, Solidity.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 32524 Statistical Optimization of Medium Components for Biomass Production of Chlorella pyrenoidosa under Autotrophic Conditions and Evaluation of Its Biochemical Composition under Stress Conditions
Authors: N. P. Dhull, K. Gupta, R. Soni, D. K. Rahi, S. K. Soni
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The aim of the present work was to statistically design an autotrophic medium for maximum biomass production by Chlorella pyrenoidosa using response surface methodology. After evaluating one factor at a time approach, K2HPO4, KNO3, MgSO4.7H2O and NaHCO3 were preferred over the other components of the fog’s medium as most critical autotrophic medium components. The study showed that the maximum biomass yield was achieved while the concentrations of MgSO4.7H2O, K2HPO4, KNO3 and NaHCO3 were 0.409 g/L, 0.24 g/L, 1.033 g/L, and 3.265 g/L, respectively. The study reported that the biomass productivity of C. pyrenoidosa improved from 0.14 g/L in defined fog’s medium to 1.40 g/L in modified fog’s medium resulting 10 fold increase. The biochemical composition biosynthesis of C. pyrenoidosa was altered using nitrogen limiting stress bringing about 5.23 fold increase in lipid content than control (cell without stress), as analyzed by FTIR integration method.
Keywords: Autotrophic condition, Chlorella pyrenoidosa, FTIR, Response Surface Methodology, Optimization.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 243923 Modeling of Titanium Alloy Implant for Fractured Distal Femur
Authors: Abhishek Soni, Bhagat Singh
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In the present work, reverse engineering (RE) approach has been used to create a 3D model of a fractured femur bone using the computed tomography (CT) scan data. Thereafter, counter fit fixation plates of Titanium alloy (Ti6Al4V) have been designed and analyzed considering physiological static loading conditions. From the analysis, it has been inferred that the stresses and deformation developed are quite low. It implies that these designed customized fixation plates are able to provide stable fixation resulting in improved fracture union.Keywords: Biomechanical evaluations, customized implant, reverse engineering, titanium alloy.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 71822 Design of Hydroxyapatite-Polyetheretherketone Fixation Plates for Diaphysis Femur Fracture
Authors: Abhishek Soni, Bhagat Singh
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In this study, scanned data of a damaged femur diaphysis are used to generate three dimensional model of the bone. Further, customized implant of Hydroxyapatite-Polyetheretherketone (HA-PEEK) material for this damaged bone is prepared using CAD modeling. Damaged bone and implant have been assembled to prepare the intact bone. This assembled model has been analyzed to evaluate the stresses and deformation developed during the static loading. It has been observed that these stresses and deformation are very less thus imply that the proposed method of preparing implant is appropriate.
Keywords: Customized implant, deformation, femur diaphysis, stress.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 54921 New Efficient Iterative Optimization Algorithm to Design the Two Channel QMF Bank
Authors: Ram Kumar Soni, Alok Jain, Rajiv Saxena
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This paper proposes an efficient method for the design of two channel quadrature mirror filter (QMF) bank. To achieve minimum value of reconstruction error near to perfect reconstruction, a linear optimization process has been proposed. Prototype low pass filter has been designed using Kaiser window function. The modified algorithm has been developed to optimize the reconstruction error using linear objective function through iteration method. The result obtained, show that the performance of the proposed algorithm is better than that of the already exists methods.Keywords: Filterbank, near perfect reconstruction, Kaiserwindow, QMF.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 167520 Simulation and Analysis of Polyetheretherketone Implants for Diaphysis Femur Fracture
Authors: Abhishek Soni, Bhagat Singh
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In the present work, reverse engineering approach has been used to create a 3D model of a fractured femur diaphysis bone using the computed tomography (CT) scan data. Thereafter, a counter fit fixation plate of polyetheretherketone (PEEK) composite has been designed and analyzed considering static physiological loading conditions. Static stress distribution and deformation analysis of the plate have been performed. From the analysis, it has been found that the stresses and deformation developed are quite low. This implies that these designed fixation plates will be able to provide stable fixation and thus resulting in improved fracture union.
Keywords: Customized implant, deformation, femur diaphysis, stress.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 68019 Modeling of Cobalt-Chromium-Molybdenum Alloy Implant for Fractured Distal Femur
Authors: Abhishek Soni, Bhagat Singh
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Distal femur fractures are the cause of abnormal gloomy. Several types of surgical treatments have been adopted by the practitioners to restore the fractured region of distal femur. Still within this domain of study, unstable fixation remains a challenge for orthopedists. In the present study, a fixation implant is designed and analyzed under physiological loading conditions for cobalt-chromium-molybdenum alloy (Co-Cr-Mo). It has been found that the stresses and deformation developed are quite low. It means that customized fixation plates will provide stable fixation resulting in improved fracture union.
Keywords: Biomechanical evaluations, customized implant, Co-Cr-Mo alloy, reverse engineering.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 75618 An Optimized Design of Non-uniform Filterbank
Authors: Ram Kumar Soni, Alok Jain, Rajiv Saxena
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The tree structured approach of non-uniform filterbank (NUFB) is normally used in perfect reconstruction (PR). The PR is not always feasible due to certain limitations, i.e, constraints in selecting design parameters, design complexity and some times output is severely affected by aliasing error if necessary and sufficient conditions of PR is not satisfied perfectly. Therefore, there has been generalized interest of researchers to go for near perfect reconstruction (NPR). In this proposed work, an optimized tree structure technique is used for the design of NPR non-uniform filterbank. Window functions of Blackman family are used to design the prototype FIR filter. A single variable linear optimization is used to minimize the amplitude distortion. The main feature of the proposed design is its simplicity with linear phase property.Keywords: Tree structure, NUFB, QMF, NPR.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 173717 Design of Polyetheretherketone Fixation Plates for Fractured Distal Femur
Authors: Abhishek Soni, Bhagat Singh
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In the present study, a methodology has been proposed to treat fracture in the distal part of the femur bone. Initially, bone model has been developed using the computed tomography scan data of the fractured bone. This information has been further used to create polyether ether ketone (PEEK) implant for this fractured bone. Damaged bone and implant models have been assembled. This assembled model has been further analyzed for stress distribution. Moreover, deformation developed was also measured. It has been observed that the stress and deformation developed was not so appreciable. Thus, it proves that the aforementioned procedure can be suitably adopted for the treatment of fractured distal femur bone.
Keywords: Distal femur, fixation plates, PEEK, reverse engineering.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 45816 Design of Stainless Steel Implant for Fractured Distal Femur
Authors: Abhishek Soni, Bhagat Singh
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Perfect restoration of fractured distal femur has been a challenging task for the medical practitioners. In the present study, model of a fractured bone has been created using the scan data of the damaged bone. Thereafter, customized implant of Stainless Steel (SS-316L) for this fractured femur bone is modeled using the reverse engineering approach. Clinical set-up is prepared by assembling all the models together. Stress and deformation analysis of this clinical set-up has been performed in order to check the load bearing capacity and intactness of the joint. From this analysis, it has been inferred that the stresses and deformation developed due to the static load of the person is within the permissible limits.
Keywords: Biomechanical evaluations, customized implant, reverse engineering, stainless steel alloy.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 80915 Observations of Conformity in the Health Professions
Authors: Tanya N. Beran, Michelle A. Drefs, Ghazwan Altabbaa, Nouf Al Harbi, Noof Al Baz, Elizabeth Oddone Paolucci
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Although interprofessional practice is a collaborative approach for problem solving among health professionals, its implementation can present challenges to its team members. In particular, they may feel pressured to agree with or conform to other members who share information that is contrary to their own understanding. Obtaining evidence of this phenomenon is challenging, as team members may underreport their conformity behaviors due to reasons such as social desirability. In this paper, a series of studies are reviewed in which several approaches to assessing conformity in the health care professions are tested. Simulations, questionnaires, and behavior checklists can be used to measure conformity behaviors. Insights from these studies show that a significant proportion of people conform either in the presence or absence of others, express a variety of verbal and nonverbal behaviors when considering whether to conform to others, may shift between conforming and moments later not conforming (and vice versa), and may not accurately report whether they conformed. A method of measuring conformity using the implicit bias test is also discussed. People at all levels in the healthcare system are encouraged to develop both formal and informal strategies to manage the conformity pressures that people face.
Keywords: Conformity, decision-making, interprofessional teams, medical simulation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 43114 A Survey of Job Scheduling and Resource Management in Grid Computing
Authors: Raksha Sharma, Vishnu Kant Soni, Manoj Kumar Mishra, Prachet Bhuyan
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Grid computing is a form of distributed computing that involves coordinating and sharing computational power, data storage and network resources across dynamic and geographically dispersed organizations. Scheduling onto the Grid is NP-complete, so there is no best scheduling algorithm for all grid computing systems. An alternative is to select an appropriate scheduling algorithm to use in a given grid environment because of the characteristics of the tasks, machines and network connectivity. Job and resource scheduling is one of the key research area in grid computing. The goal of scheduling is to achieve highest possible system throughput and to match the application need with the available computing resources. Motivation of the survey is to encourage the amateur researcher in the field of grid computing, so that they can understand easily the concept of scheduling and can contribute in developing more efficient scheduling algorithm. This will benefit interested researchers to carry out further work in this thrust area of research.Keywords: Grid Computing, Job Scheduling, ResourceScheduling.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 340913 Application of Ti/RuO2-SnO2-Sb2O5 Anode for Degradation of Reactive Black-5 Dye
Authors: Jayesh P. Ruparelia, Bhavna D. Soni
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Electrochemical-oxidation of Reactive Black-5 (RB- 5) was conducted for degradation using DSA type Ti/RuO2-SnO2- Sb2O5 electrode. In the study, for electro-oxidation, electrode was indigenously fabricated in laboratory using titanium as substrate. This substrate was coated using different metal oxides RuO2, Sb2O5 and SnO2 by thermal decomposition method. Laboratory scale batch reactor was used for degradation and decolorization studies at pH 2, 7 and 11. Current density (50mA/cm2) and distance between electrodes (8mm) were kept constant for all experiments. Under identical conditions, removal of color, COD and TOC at initial pH 2 was 99.40%, 55% and 37% respectively for initial concentration of 100 mg/L RB-5. Surface morphology and composition of the fabricated electrode coatings were characterized using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) respectively. Coating microstructure was analyzed by X-ray diffraction (XRD). Results of this study further revealed that almost 90% of oxidation occurred within 5-10 minutes.
Keywords: Electrochemical-oxidation, RB- dye, Decolorization.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 268212 Energy Efficient Clustering and Data Aggregation in Wireless Sensor Networks
Authors: Surender Kumar Soni
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Wireless Sensor Networks (WSNs) are wireless networks consisting of number of tiny, low cost and low power sensor nodes to monitor various physical phenomena like temperature, pressure, vibration, landslide detection, presence of any object, etc. The major limitation in these networks is the use of nonrechargeable battery having limited power supply. The main cause of energy consumption WSN is communication subsystem. This paper presents an efficient grid formation/clustering strategy known as Grid based level Clustering and Aggregation of Data (GCAD). The proposed clustering strategy is simple and scalable that uses low duty cycle approach to keep non-CH nodes into sleep mode thus reducing energy consumption. Simulation results demonstrate that our proposed GCAD protocol performs better in various performance metrics.Keywords: Ad hoc network, Cluster, Grid base clustering, Wireless sensor network.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 313611 Energy Map Construction using Adaptive Alpha Grey Prediction Model in WSNs
Authors: Surender Kumar Soni, Dhirendra Pratap Singh
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Wireless Sensor Networks can be used to monitor the physical phenomenon in such areas where human approach is nearly impossible. Hence the limited power supply is the major constraint of the WSNs due to the use of non-rechargeable batteries in sensor nodes. A lot of researches are going on to reduce the energy consumption of sensor nodes. Energy map can be used with clustering, data dissemination and routing techniques to reduce the power consumption of WSNs. Energy map can also be used to know which part of the network is going to fail in near future. In this paper, Energy map is constructed using the prediction based approach. Adaptive alpha GM(1,1) model is used as the prediction model. GM(1,1) is being used worldwide in many applications for predicting future values of time series using some past values due to its high computational efficiency and accuracy.Keywords: Adaptive Alpha GM(1, 1) Model, Energy Map, Prediction Based Data Reduction, Wireless Sensor Networks
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 180010 Grouping-Based Job Scheduling Model In Grid Computing
Authors: Vishnu Kant Soni, Raksha Sharma, Manoj Kumar Mishra
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Grid computing is a high performance computing environment to solve larger scale computational applications. Grid computing contains resource management, job scheduling, security problems, information management and so on. Job scheduling is a fundamental and important issue in achieving high performance in grid computing systems. However, it is a big challenge to design an efficient scheduler and its implementation. In Grid Computing, there is a need of further improvement in Job Scheduling algorithm to schedule the light-weight or small jobs into a coarse-grained or group of jobs, which will reduce the communication time, processing time and enhance resource utilization. This Grouping strategy considers the processing power, memory-size and bandwidth requirements of each job to realize the real grid system. The experimental results demonstrate that the proposed scheduling algorithm efficiently reduces the processing time of jobs in comparison to others.Keywords: Grid computing, Job grouping and Jobscheduling.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19459 Smart Motion
Authors: Arpita Soni, Sapna Mittal
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Austenite and Martensite indicate the phases of solids undergoing phase transformation which we usually associate with materials and not with living organisms. This article provides an overview of bacterial proteins and structures that are undergoing phase transformation and suggests its probable effect on mechanical behavior. The context is mainly within the role of phase transformations occurring in the flagellum of bacteria. The current knowledge of molecular mechanism leading to phase variation in living organisms is reviewed. Since in bacteria, each flagellum is driven by a separate motor, similarity to a Differential drive in case of four-wheeled vehicles is suggested. It also suggests the application of the mechanism in which bacteria changes its direction of movement to facilitate single point turning of a multi-wheeled vehicle. Finally, examples are presented to illustrate that the motion due to phase transformation of flagella in bacteria can start a whole new research on motion mechanisms.
Keywords: Flagella, Phase Transformation, Nanobots, Differential Drive, Single point turn, Biomimetics
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15688 Industrial Production and Clinical Application of L-Asparaginase: A Chemotherapeutic Agent
Authors: Soni Yadav, Sitansu Kumar Verma, Jitendra Singh, Ajay Kumar
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This article comprises detail information about L-asparaginase, encompassing topic such as various sources of L-asparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of L-asparaginase along with information about the application of L-asparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid and ammonia. Asparagine is a nutritional requirement for both normal and tumor cell. Present scenario has found that L-asparaginase has been found to be a best anti tumor or antileukemic agent. In the recent years this enzyme gained application in the field of clinical research pharmacologic and food industry. It has been characterized based on the enzyme assay principle hydrolyzing L-asparagine into L-aspartic acid and ammonia. It has been observed that eukaryotic microorganisms such as yeast and filamentous fungi have a potential for L-asparaginase production. L-asparaginase has been and is still one of the most lengthily studied therapeutic enzymes by scientist and researchers worldwide.
Keywords: L-asparaginase, antitumor, solid state fermentation, chemotherapeutic.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 69137 Sericulture for Employment Generation among the Tribal- A Study of Two Trible Block of Raigarh Dist. [C.G.] India
Authors: S. K. Dewangan, K. R. Sahu, S. K. Patnayak, S. Soni
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Among many agro- based cottage industries in India sericulture has been promoted as an agro-based, labor intensive, rural oriented cottage industry, providing gainful employment mainly to the weaker and marginalized section of the society specially tribal. Sericulture occupies the place of pride in the rural economy can be practiced even with very low land holding, low gestation, high returns make sericulture an ideal program, requiring little capital investment. In 2010-2011 the employment in sericulture sector was 72.5 lakh persons. The involvement of landless rural people in tasar sericulture is because they understood its potential for rural and tribal upliftment. This article demonstrates that certain developmental initiatives have been playing an important role in the socio-economic progress of tribal masses in Raigarh district and explains the increased returns from sericulture as a result of development programs. The study concludes with some suggestions to improve the long term feasibility of sericulture.
Keywords: Development, Employment, Income, Sericulture, Tribal, Tasar,
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 30496 Protection of Transformers against Surge Voltage
Authors: Anil S. Khopkar, Umesh N. Soni
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Surge voltage arises in the system either by switching operations of heavy load or by natural lightning. Surge voltages cause significant failure of power system equipment if adequate protection not provided. The surge arrestor is device which is connected in a power system to protect the equipment against surge voltages. To protect the transformers against surge voltages, metal oxide surge arrestors (MOSA) are connected across each terminal. The Basic Insulation Level (BIL) of transformers has been defined in the national and international standards based on its voltage rating. While designing transformer insulation, factors such as BIL, surge arrestor ratings, and its operating voltage have to be considered. However, the performance of transformer insulation largely depends on the ratings of the surge arrestor ratings, their location, the margin considered in insulation design, the quantity of surge voltage strikes, etc. This paper demonstrates the role of surge arrestors in protecting transformers against overvoltage, transformer insulation design, the optimum location of surge arrestors and their connection lead length, insulation coordination for transformers, the protection margin in BIL, and methods of safeguarding transformers against surge voltages in detail.
Keywords: Surge voltage, surge arrestors, transformer, protection margin.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 775 An Agent Based Dynamic Resource Scheduling Model with FCFS-Job Grouping Strategy in Grid Computing
Authors: Raksha Sharma, Vishnu Kant Soni, Manoj Kumar Mishra, Prachet Bhuyan, Utpal Chandra Dey
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Grid computing is a group of clusters connected over high-speed networks that involves coordinating and sharing computational power, data storage and network resources operating across dynamic and geographically dispersed locations. Resource management and job scheduling are critical tasks in grid computing. Resource selection becomes challenging due to heterogeneity and dynamic availability of resources. Job scheduling is a NP-complete problem and different heuristics may be used to reach an optimal or near optimal solution. This paper proposes a model for resource and job scheduling in dynamic grid environment. The main focus is to maximize the resource utilization and minimize processing time of jobs. Grid resource selection strategy is based on Max Heap Tree (MHT) that best suits for large scale application and root node of MHT is selected for job submission. Job grouping concept is used to maximize resource utilization for scheduling of jobs in grid computing. Proposed resource selection model and job grouping concept are used to enhance scalability, robustness, efficiency and load balancing ability of the grid.Keywords: Agent, Grid Computing, Job Grouping, Max Heap Tree (MHT), Resource Scheduling.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20884 Three-Dimensional, Non-Linear Finite Element Analysis of Bullet Penetration through Thin AISI 4340 Steel Target Plate
Authors: Abhishek Soni, A. Kumaraswamy, M. S. Mahesh
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Bullet penetration in steel plate is investigated with the help of three-dimensional, non-linear, transient, dynamic, finite elements analysis using explicit time integration code LSDYNA. The effect of large strain, strain-rate and temperature at very high velocity regime was studied from number of simulations of semi-spherical nose shape bullet penetration through single layered circular plate with 2 mm thickness at impact velocities of 500, 1000, and 1500 m/s with the help of Johnson Cook material model. Mie-Gruneisen equation of state is used in conjunction with Johnson Cook material model to determine pressure-volume relationship at various points of interests. Two material models viz. Plastic-Kinematic and Johnson- Cook resulted in different deformation patterns in steel plate. It is observed from the simulation results that the velocity drop and loss of kinetic energy occurred very quickly up to perforation of plate, after that the change in velocity and changes in kinetic energy are negligibly small. The physics behind this kind of behaviour is presented in the paper.Keywords: AISI 4340 steel, ballistic impact simulation, bullet penetration, non-linear FEM.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 12633 Application of Statistical Approach for Optimizing CMCase Production by Bacillus tequilensis S28 Strain via Submerged Fermentation Using Wheat Bran as Carbon Source
Authors: A. Sharma, R. Tewari, S. K. Soni
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Biofuels production has come forth as a future technology to combat the problem of depleting fossil fuels. Bio-based ethanol production from enzymatic lignocellulosic biomass degradation serves an efficient method and catching the eye of scientific community. High cost of the enzyme is the major obstacle in preventing the commercialization of this process. Thus main objective of the present study was to optimize composition of medium components for enhancing cellulase production by newly isolated strain of Bacillus tequilensis. Nineteen factors were taken into account using statistical Plackett-Burman Design. The significant variables influencing the cellulose production were further employed in statistical Response Surface Methodology using Central Composite Design for maximizing cellulase production. The optimum medium composition for cellulase production was: peptone (4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L), Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride (0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at 37oC. Analysis of variance (ANOVA) revealed high coefficient of determination (R2) of 0.99. Maximum cellulase productivity of 11.5 IU/ml was observed against the model predicted value of 13 IU/ml. This was found to be optimally active at 60oC and pH 5.5.
Keywords: Bacillus tequilensis, CMCase, Submerged Fermentation, Optimization, Plackett-Burman Design, Response Surface Methodology.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 30622 Web–Based Tools and Databases for Micro-RNA Analysis: A Review
Authors: Sitansu Kumar Verma, Soni Yadav, Jitendra Singh, Shraddha, Ajay Kumar
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MicroRNAs (miRNAs), a class of approximately 22 nucleotide long non coding RNAs which play critical role in different biological processes. The mature microRNA is usually 19–27 nucleotides long and is derived from a bigger precursor that folds into a flawed stem-loop structure. Mature micro RNAs are involved in many cellular processes that encompass development, proliferation, stress response, apoptosis, and fat metabolism by gene regulation. Resent finding reveals that certain viruses encode their own miRNA that processed by cellular RNAi machinery. In recent research indicate that cellular microRNA can target the genetic material of invading viruses. Cellular microRNA can be used in the virus life cycle; either to up regulate or down regulate viral gene expression Computational tools use in miRNA target prediction has been changing drastically in recent years. Many of the methods have been made available on the web and can be used by experimental researcher and scientist without expert knowledge of bioinformatics. With the development and ease of use of genomic technologies and computational tools in the field of microRNA biology has superior tremendously over the previous decade. This review attempts to give an overview over the genome wide approaches that have allow for the discovery of new miRNAs and development of new miRNA target prediction tools and databases.
Keywords: MicroRNAs, computational tools, gene regulation, databases, RNAi.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 31821 A β-mannanase from Fusarium oxysporum SS-25 via Solid State Fermentation on Brewer’s Spent Grain: Medium Optimization by Statistical Tools, Kinetic Characterization and Its Applications
Authors: S. S. Rana, C. Janveja, S. K. Soni
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This study is concerned with the optimization of fermentation parameters for the hyper production of mannanase from Fusarium oxysporum SS-25 employing two step statistical strategy and kinetic characterization of crude enzyme preparation. The Plackett-Burman design used to screen out the important factors in the culture medium revealed 20% (w/w) wheat bran, 2% (w/w) each of potato peels, soyabean meal and malt extract, 1% tryptone, 0.14% NH4SO4, 0.2% KH2PO4, 0.0002% ZnSO4, 0.0005% FeSO4, 0.01% MnSO4, 0.012% SDS, 0.03% NH4Cl, 0.1% NaNO3 in brewer’s spent grain based medium with 50% moisture content, inoculated with 2.8×107 spores and incubated at 30oC for 6 days to be the main parameters influencing the enzyme production. Of these factors, four variables including soyabean meal, FeSO4, MnSO4 and NaNO3 were chosen to study the interactive effects and their optimum levels in central composite design of response surface methodology with the final mannanase yield of 193 IU/gds. The kinetic characterization revealed the crude enzyme to be active over broader temperature and pH range. This could result in 26.6% reduction in kappa number with 4.93% higher tear index and 1% increase in brightness when used to treat the wheat straw based kraft pulp. The hydrolytic potential of enzyme was also demonstrated on both locust bean gum and guar gum.
Keywords: Brewer’s Spent Grain, Fusarium oxysporum, Mannanase, Response Surface Methodology.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 5173