Search results for: Ad-hoc On-demand Distance Vector

Authors: Harshit Mehrotra, Gaurav Agrawal, M.C. Srivastava

Abstract:

Computerized lip reading has been one of the most actively researched areas of computer vision in recent past because of its crime fighting potential and invariance to acoustic environment. However, several factors like fast speech, bad pronunciation, poor illumination, movement of face, moustaches and beards make lip reading difficult. In present work, we propose a solution for automatic lip contour tracking and recognizing letters of English language spoken by speakers using the information available from lip movements. Level set method is used for tracking lip contour using a contour velocity model and a feature vector of lip movements is then obtained. Character recognition is performed using modified k nearest neighbor algorithm which assigns more weight to nearer neighbors. The proposed system has been found to have accuracy of 73.3% for character recognition with speaker lip movements as the only input and without using any speech recognition system in parallel. The approach used in this work is found to significantly solve the purpose of lip reading when size of database is small.

Keywords: Contour Velocity Model, Lip Contour Tracking, LipReading, Visual Character Recognition.

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Authors: Jiří Barta, Oldřich Svoboda, Jiří. F. Urbánek

Abstract:

In this paper will be discussed two coin´s sides of crisis scenarios dynamics. On the one's side is negative role of subsidiary scenario branches in its compactness weakening by means unduly chaotic atomizing, having many interactive feedbacks cases, increasing a value of a complexity here. This negative role reflects the complexity of use cases, weakening leader compliancy, which brings something as a ´readiness for controlling capabilities provision´. Leader´s dissatisfaction has zero compliancy, but factual it is a ´crossbar´ (interface in fact) between planning and executing use cases. On the other side of this coin, an advantage of rich scenarios embranchment is possible to see in a support of response awareness, readiness, preparedness, adaptability, creativity and flexibility. Here rich scenarios embranchment contributes to the steadiness and resistance of scenario mission actors. These all will be presented in live power-points ´Blazons´, modelled via DYVELOP (Dynamic Vector Logistics of Processes) on the Conference.

Keywords: Leadership, Controlling, Complexity, DYVELOP, Scenarios.

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Authors: P. Srinivas, P. V. N. Prasad

Abstract:

Since torque ripple is the main cause of noise and vibrations, the performance of Switched Reluctance Motor (SRM) can be improved by minimizing its torque ripple using a novel control technique called Direct Torque Control (DTC). In DTC technique, torque is controlled directly through control of magnitude of the flux and change in speed of the stator flux vector. The flux and torque are maintained within set hysteresis bands.

The DTC of SRM is analyzed by two methods. In one method, the actual torque is computed by conducting Finite Element Analysis (FEA) on the design specifications of the motor. In the other method, the torque is computed by Simplified Torque Equation. The variation of peak current, average current, torque ripple and speed settling time with Simplified Torque Equation model is compared with FEA based model.

Keywords: Direct Toque Control, Simplified Torque Equation, Finite Element Analysis, Torque Ripple.

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Authors: Fayssal Amrane, Azeddine Chaiba

Abstract:

In this paper, type-2 fuzzy logic control (T2FLC) and neuro-fuzzy control (NFC) for a doubly fed induction generator (DFIG) based on direct power control (DPC) with a fixed switching frequency is proposed for wind generation application. First, a mathematical model of the doubly-fed induction generator implemented in d-q reference frame is achieved. Then, a DPC algorithm approach for controlling active and reactive power of DFIG via fixed switching frequency is incorporated using PID. The performance of T2FLC and NFC, which is based on the DPC algorithm, are investigated and compared to those obtained from the PID controller. Finally, simulation results demonstrate that the NFC is more robust, superior dynamic performance for wind power generation system applications.

Keywords: Doubly fed induction generetor, direct power control, space vector modulation, type-2 fuzzy logic control, neuro-fuzzy control, maximum power point tracking.

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Authors: B. Munro, D. Lim, A. Anvar

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This paper describes a feasibility study that is included with the research, development and testing of a micro communications sonobuoy deployable by Maritime Fixed wing Unmanned Aerial Vehicles (M-UAV) and rotor wing Quad Copters which are both currently being developed by the University of Adelaide. The micro communications sonobuoy is developed to act as a seamless communication relay between an Autonomous Underwater Vehicle (AUV) and an above water human operator some distance away. Development of such a device would eliminate the requirement of physical communication tethers attached to submersible vehicles for control and data retrieval.

Keywords: Autonomous Underwater Vehicle, AUV, Maritime, Unmanned Aerial Vehicle, UAV, Micro Sonobuoy, Communication.

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Authors: K. Sheehy, R. Ferguson, G. Clough

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This paper examines ethical and social issues which have proved important when initiating and creating educational spaces within a virtual environment. It focuses on one project, identifying the key decisions made, the barriers to new practice encountered and the impact these had on the project. It demonstrates the importance of the 'backstage' ethical and social issues involved in the creation of a virtual education community and offers conclusions, and questions, which will inform future research and practice in this area. These ethical issues are considered using Knobel-s framework of front-end, in-process and back-end concerns, and include establishing social practices for the islands, allocating access rights, considering personal safety and supporting researchers appropriately within this context.

Keywords: distance education, ethics, virtual environments.

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Authors: Ishtiaq A. Chaudhry, Zia R. Tahir

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Experimental investigation has been carried out towards understanding the complex fluid dynamics involved in the interaction of vortical structures with zero pressure gradient boundary layer. A laminar boundary layer is produced on the flat plate placed in the water flume and the synthetic jet actuator is deployed on top of the plate at a definite distance from the leading edge. The synthetic jet actuator has been designed in such a way that the to and fro motion of the diaphragm is maneuvered at will by varying the operating parameters to produce the typical streamwise vortical structures namely hairpin and tilted vortices. PIV measurements are made on the streamwise plane normal to the plate to evaluate their interaction with the near wall fluid.

Keywords: Boundary layer, synthetic jet actuator, flow separation control, vortical structures.

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Authors: Ayhan Aydoğdu, Ercan Ceyhan, Ali Kahraman, Nursel Çöl

Abstract:

This research was conducted to determine effects of plant densities on seed yield and some agricultural characteristics of pea variety- Jofs in Konya ecological conditions during 2012 vegetation period. The trial was set up according to “Randomized Blocks Design” with three replications. The material “Jofs” pea variety was subjected to 3-row spaces (30, 40 and 50 cm) and 3-row distances (5, 10 and 15 cm). According to the results, difference was shown statistically for the effects of row spaces and row distances on seed yield. The highest seed yield was 2582.1 kg ha-1 on 30 cm of row spaces while 2562.2 kg ha-1 on 15 cm of distances. Consequently, the optimum planting density was determined as 30 x 15 cm for Jofs pea variety growing in Konya.

Keywords: Pea, row space, row distance, seed yield.

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Authors: S. H. Anilkumar, Ghulam Jilani

Abstract:

The objective of the present work is to conduct investigations leading to a more complete explanation of single phase natural convective heat transfer in an enclosure with fin utilizing nano fluids. The nano fluid used, which is composed of Aluminum oxide nano particles in suspension of Ethylene glycol, is provided at various volume fractions. The study is carried out numerically for a range of Rayleigh numbers, fin heights and aspect ratio. The flow and temperature distributions are taken to be two-dimensional. Regions with the same velocity and temperature distributions are identified as symmetry of sections. One half of such a rectangular region is chosen as the computational domain taking into account the symmetry about the fin. Transport equations are modeled by a stream functionvorticity formulation and are solved numerically by finite-difference schemes. Comparisons with previously published works on the basis of special cases are done. Results are presented in the form of streamline, vector and isotherm plots as well as the variation of local Nusselt number along the fin under different conditions.

Keywords: Fin height, Nano fluid, natural convection, Rayleigh number.

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Authors: K. Raghuwaiya, B. Sharma, J. Vanualailai

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This paper presents a set of artificial potential field functions that improves upon, in general, the motion planning and posture control, with theoretically guaranteed point and posture stabilities, convergence and collision avoidance properties of the general3-trailer system in a priori known environment. We basically design and inject two new concepts; ghost walls and the distance optimization technique (DOT) to strengthen point and posture stabilities, in the sense of Lyapunov, of our dynamical model. This new combination of techniques emerges as a convenient mechanism for obtaining feasible orientations at the target positions with an overall reduction in the complexity of the navigation laws. Simulations are provided to demonstrate the effectiveness of the controls laws.

Keywords: Artificial potential fields, 3-trailer systems, motion planning, posture.

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Authors: Vesile Evrim, Aliyu Awwal

Abstract:

By the evolvement in technology, the way of expressing opinions switched direction to the digital world. The domain of politics, as one of the hottest topics of opinion mining research, merged together with the behavior analysis for affiliation determination in texts, which constitutes the subject of this paper. This study aims to classify the text in news/blogs either as Republican or Democrat with the minimum number of features. As an initial set, 68 features which 64 were constituted by Linguistic Inquiry and Word Count (LIWC) features were tested against 14 benchmark classification algorithms. In the later experiments, the dimensions of the feature vector reduced based on the 7 feature selection algorithms. The results show that the “Decision Tree”, “Rule Induction” and “M5 Rule” classifiers when used with “SVM” and “IGR” feature selection algorithms performed the best up to 82.5% accuracy on a given dataset. Further tests on a single feature and the linguistic based feature sets showed the similar results. The feature “Function”, as an aggregate feature of the linguistic category, was found as the most differentiating feature among the 68 features with the accuracy of 81% in classifying articles either as Republican or Democrat.

Keywords: Politics, machine learning, feature selection, LIWC.

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Authors: P. K. Rahul Krishna, S. Sandeep Kumar, Jayanthi Sunder Raj

Abstract:

The article explores one of the important relations between numbers-the Pythagorean triples (triplets) which finds its application in distance measurement, construction of roads, towers, buildings and wherever Pythagoras theorem finds its application. The Pythagorean triples are numbers, that satisfy the condition “In a given set of three natural numbers, the sum of squares of two natural numbers is equal to the square of the other natural number”. There are numerous methods and equations to obtain the triplets, which have their own merits and demerits. Here, quadratic approach for generating triples uses the hypotenuse leg difference method. The advantage is that variables are few and finally only three independent variables are present.

Keywords: Arithmetic progression, hypotenuse leg difference method, natural numbers, Pythagorean triplets, quadratic equation.

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Authors: Kamalpreet Kaur, Harjit Pal Singh, Vikas Khullar

Abstract:

In Wireless Sensor Network (WSN), the main limitation is generally inimitable energy consumption during processing of the sensor nodes. Cluster head (CH) election is one of the main issues that can reduce the energy consumption. Therefore, discovering energy saving routing protocol is the focused area for research. In this paper, fuzzy-based energy aware routing protocol is presented, which enhances the stability and network lifetime of the network. Fuzzy logic ensures the well-organized selection of CH by taking four linguistic variables that are concentration, energy, centrality, and distance to base station (BS). The results show that the proposed protocol shows better results in requisites of stability and throughput of the network.

Keywords: ERA, fuzzy logic, network model, WSN.

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Authors: S. Venkateshwarlu, B. P. Muni, A. D. Rajkumar, J. Praveen

Abstract:

Custom power is a technology driven product and service solution which embraces a family devices such as Dynamic Voltage Restorer (DVR), Distributed Shunt Compensator (DSTATCOM), Solid State Breaker (SSB) etc which will provide power quality functions at distribution voltages. The rapid response of these devices enables them to operate in real time, providing continuous and dynamic control of the supply including voltage and reactive power regulation, harmonic reduction and elimination of voltage dips. This paper presents the benefits of multilevel inverters when they are used for DPC based custom power devices. Power flow control mechanism, salient features, advantages and disadvantages of direct power control (DPC) using lookup table, SVM, predictive voltage vector and hybrid DPC strategies are discussed in this paper. Simulation results of three level inverter based STATCOM, harmonic analysis of multi level inverters are presented at the end.

Keywords: DPC, DPC-SVM, Dynamic voltage restorer, DSTATCOM, Multilevel inverter, PWM Converter, PDPC, VF-DPC.

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Authors: Yen-Po Wang, Ming-Chih Huang, Ming-Lian Chang

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A scheme integrated with deterministic–stochastic subspace system identification and the method of damage localization vector is proposed in this study for damage detection of structures based on seismic response data. A series of shaking table tests using a five-storey steel frame has been conducted in National Center for Research on Earthquake Engineering (NCREE), Taiwan. Damage condition is simulated by reducing the cross-sectional area of some of the columns at the bottom. Both single and combinations of multiple damage conditions at various locations have been considered. In the system identification analysis, either full or partial observation conditions have been taken into account. It has been shown that the damaged stories can be identified from global responses of the structure to earthquakes if sufficiently observed. In addition to detecting damage(s) with respect to the intact structure, identification of new or extended damages of the as-damaged (ill-conditioned) counterpart has also been studied. The proposed scheme proves to be effective.

Keywords: Damage locating vectors, deterministic-stochastic subspace system, shaking table tests, system identification.

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Authors: T. Hudecek, Z. Zakova

Abstract:

The paper deals with cartographic visualisation of results of transport accessibility monitoring with the use of a semiautomated method of unipolar anamorphosis, developed by the authors in the GIS environment. The method is based on transformation of distance in the map to values of a geographical phenomenon. In the case of time accessibility it is based on transformation of isochrones converted into the form of concentric circles, taking into account selected topographic and thematic elements in the map. The method is most suitable for analyses of accessibility to or from a centre and for modelling its long-term context. The paper provides a detailed analysis of the procedures and functionality of the method, discussing the issues of coordinates, transformation, scale and visualisation. It also offers a discussion of possible problems and inaccuracies. A practical application of the method is illustrated by previous research results by the authors in the filed of accessibility in Czechia.

Keywords: accessibility, GIS, transformation, unipolar anamorphosis

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Authors: U. Bottigli, D.Cascio, F. Fauci, B. Golosio, R. Magro, G.L. Masala, P. Oliva, G. Raso, S.Stumbo

Abstract:

Purpose of this work is the development of an automatic classification system which could be useful for radiologists in the investigation of breast cancer. The software has been designed in the framework of the MAGIC-5 collaboration. In the automatic classification system the suspicious regions with high probability to include a lesion are extracted from the image as regions of interest (ROIs). Each ROI is characterized by some features based on morphological lesion differences. Some classifiers as a Feed Forward Neural Network, a K-Nearest Neighbours and a Support Vector Machine are used to distinguish the pathological records from the healthy ones. The results obtained in terms of sensitivity (percentage of pathological ROIs correctly classified) and specificity (percentage of non-pathological ROIs correctly classified) will be presented through the Receive Operating Characteristic curve (ROC). In particular the best performances are 88% ± 1 of area under ROC curve obtained with the Feed Forward Neural Network.

Keywords: Neural Networks, K-Nearest Neighbours, SupportVector Machine, Computer Aided Diagnosis.

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Authors: Sami Mutair, Yasuyuki Ikegami

Abstract:

This paper reports on the results of experimental investigations of flash evaporation from superheated jet issues vertically upward from a round straight nozzle of 81.3 mm diameter. For the investigated range of jet superheat degree and velocity, it was shown that flash evaporation enhances with initial temperature increase. Due to the increase of jet inertia and subsequently the delay of jet shattering, increase of jet velocity was found to result in increase of evaporation "delay period". An empirical equation predicts the jet evaporation completion height was developed, this equation is thought to be useful in designing the flash evaporation chamber. In attempts for enhancement of flash evaporation, use of steel wire mesh located at short distance downstream was found effective with no consequent pressure drop.

Keywords: Enhancement; Flash Evaporation; OTEC; superheated jet

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Authors: J. Soltani, H. Abootorabi Zarchi, Gh. R. Arab Markadeh

Abstract:

In this paper a sliding-mode torque and flux control is designed for encoderless synchronous reluctance motor drive. The sliding-mode plus PI controllers are designed in the stator-flux field oriented reference frame which is able to track the mentioned reference signals with a minimum pulsations in the state condition. In addition, with these controllers a fast dynamic response is also achieved for the drive system. The proposed control scheme is robust subject to parameters variation except to stator resistance. To solve this problem a simple estimator is used for on-line detecting of this parameter. Moreover, the rotor position and speed are estimated by on-line obtaining of the stator-flux-space vector. The effectiveness and capability of the proposed control approach is verified by both the simulation and experimental results.

Keywords: Synchronous Reluctance Motor, Direct Torque and Flux Control, Sliding Mode, Field-Oriented Frame, Encoderless.

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Authors: Samson Okwakol Ariko, Venansius Baryamureeba

Abstract:

Internet Access Technologies (IAT) provide a means through which Internet can be accessed. The choice of a suitable Internet technology is increasingly becoming an important issue to ISP clients. Currently, the choice of IAT is based on discretion and intuition of the concerned managers and the reliance on ISPs. In this paper we propose a model and designs algorithms that are used in the Internet access technology specification. In the proposed model, three ranking approaches are introduced; concurrent ranking, stepwise ranking and weighted ranking. The model ranks the IAT based on distance measures computed in ascending order while the global ranking system assigns weights to each IAT according to the position held in each ranking technique, determines the total weight of a particular IAT and ranks them in descending order. The final output is an objective ranking of IAT in descending order.

Keywords: Internet Access Technology (IAT).

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Authors: S. Nandagopalan, Dr. B. S. Adiga, N. Deepak

Abstract:

In this paper a novel approach for generalized image retrieval based on semantic contents is presented. A combination of three feature extraction methods namely color, texture, and edge histogram descriptor. There is a provision to add new features in future for better retrieval efficiency. Any combination of these methods, which is more appropriate for the application, can be used for retrieval. This is provided through User Interface (UI) in the form of relevance feedback. The image properties analyzed in this work are by using computer vision and image processing algorithms. For color the histogram of images are computed, for texture cooccurrence matrix based entropy, energy, etc, are calculated and for edge density it is Edge Histogram Descriptor (EHD) that is found. For retrieval of images, a novel idea is developed based on greedy strategy to reduce the computational complexity. The entire system was developed using AForge.Imaging (an open source product), MATLAB .NET Builder, C#, and Oracle 10g. The system was tested with Coral Image database containing 1000 natural images and achieved better results.

Keywords: Content Based Image Retrieval (CBIR), Cooccurrencematrix, Feature vector, Edge Histogram Descriptor(EHD), Greedy strategy.

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Authors: Lazim Abdullah, Nurhakimah Ab. Rahman

Abstract:

Linear systems are widely used in many fields of science and engineering. In many applications, at least some of the parameters of the system are represented by fuzzy rather than crisp numbers. Therefore it is important to perform numerical algorithms or procedures that would treat general fuzzy linear systems and solve them using iterative methods. This paper aims are to solve fuzzy linear systems using four types of Jacobi based iterative methods. Four iterative methods based on Jacobi are used for solving a general n × n fuzzy system of linear equations of the form Ax = b , where A is a crisp matrix and b an arbitrary fuzzy vector. The Jacobi, Jacobi Over-Relaxation, Refinement of Jacobi and Refinement of Jacobi Over-Relaxation methods was tested to a five by five fuzzy linear system. It is found that all the tested methods were iterated differently. Due to the effect of extrapolation parameters and the refinement, the Refinement of Jacobi Over-Relaxation method was outperformed the other three methods.

Keywords: Fuzzy linear systems, Jacobi, Jacobi Over- Relaxation, Refinement of Jacobi, Refinement of Jacobi Over- Relaxation.

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Authors: Sanghyeok Oh, Keechul Jung

Abstract:

Although lots of research work has been done for human pose recognition, the view-point of cameras is still critical problem of overall recognition system. In this paper, view-point insensitive human pose recognition is proposed. The aims of the proposed system are view-point insensitivity and real-time processing. Recognition system consists of feature extraction module, neural network and real-time feed forward calculation. First, histogram-based method is used to extract feature from silhouette image and it is suitable for represent the shape of human pose. To reduce the dimension of feature vector, Principle Component Analysis(PCA) is used. Second, real-time processing is implemented by using Compute Unified Device Architecture(CUDA) and this architecture improves the speed of feed-forward calculation of neural network. We demonstrate the effectiveness of our approach with experiments on real environment.

Keywords: computer vision, neural network, pose recognition, view-point insensitive, PCA, CUDA.

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Authors: Stephen Akuma, Timothy Ndera

Abstract:

Information seekers get “lost in hyperspace” due to the voluminous documents updated daily on the internet. Adaptive Hypermedia Systems (AHS) are used to direct learners to their target goals. One of the most common AHS designed to help information seekers to overcome the problem of information overload is the Adaptive Education Hypermedia System (AEHS). However, this paper focuses on AEHS that adopts the learning preference of high school students and deliver learning content according to this preference throughout their learning experience. The research developed a prototype system for predicting students’ learning preference from the Visual, Aural, Read-Write and Kinesthetic (VARK) learning style model and adopting the learning content suitable to their preference. The predicting strength of several classifiers was compared and we found Support Vector Machine (SVM) to be more accurate in predicting learning style based on users’ preferences.

Keywords: Hypermedia, adaptive education, learning style, lesson content, user profile, prediction, feedback, adaptive hypermedia, learning style.

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Authors: Manash Pratim Sarma, Kandarpa Kumar Sarma

Abstract:

A set of Artificial Neural Network (ANN) based methods for the design of an effective system of speech recognition of numerals of Assamese language captured under varied recording conditions and moods is presented here. The work is related to the formulation of several ANN models configured to use Linear Predictive Code (LPC), Principal Component Analysis (PCA) and other features to tackle mood and gender variations uttering numbers as part of an Automatic Speech Recognition (ASR) system in Assamese. The ANN models are designed using a combination of Self Organizing Map (SOM) and Multi Layer Perceptron (MLP) constituting a Learning Vector Quantization (LVQ) block trained in a cooperative environment to handle male and female speech samples of numerals of Assamese- a language spoken by a sizable population in the North-Eastern part of India. The work provides a comparative evaluation of several such combinations while subjected to handle speech samples with gender based differences captured by a microphone in four different conditions viz. noiseless, noise mixed, stressed and stress-free.

Keywords: Assamese, Recognition, LPC, Spectral, ANN.

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Authors: A. Niknejad, G. H. Liaghat, A. H. Behravesh, H. Moslemi Naeini

Abstract:

In this paper, a semi empirical formula is presented based on the experimental results to predict the first pick (maximum force) value in the instantaneous folding force- axial distance diagram of a square column. To achieve this purpose, the maximum value of the folding force was assumed to be a function of the average folding force. Using the experimental results, the maximum value of the force necessary to initiate the first fold in a square column was obtained with respect to the geometrical quantities and material properties. Finally, the results obtained from the semi empirical relation in this paper, were compared to the experimental results which showed a good correlation.

Keywords: Honeycomb, folding force, square column, aluminum, axial loading.

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Authors: Nishanth Murugan, Mohammed Niyasdeen Nejaamtheen, S. Sounder Rajan

Abstract:

The accelerated growth in aircraft industries desire effectual schemes, programs, innovative designs of advanced systems to accomplishing the augmenting need for home-free air transportation. In this paper, a contemporary conceptual design of an airplane has been proposed without landing gear systems in order to reducing accidents, time consumption, and to eliminating drawbacks by using superconducting levitation phenomenon. This invention of an airplane with superconductive material coating, on the solar plexus region assist to reduce weight by approximately 4% of the total takeoff weight, and cost effective. Moreover, we conjectured that superconductor landing system reduces ground friction, mission fuel, total drag, take-off and landing distance.

Keywords: Aircraft landing system, Magnetic levitation, Superconductors, Take-off and landing.

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Authors: H. Dujmic, V. Papic, H. Turic

Abstract:

The article presents a new method for detection of artificial objects and materials from images of the environmental (non-urban) terrain. Our approach uses the hue and saturation (or Cb and Cr) components of the image as the input to the segmentation module that uses the mean shift method. The clusters obtained as the output of this stage have been processed by the decision-making module in order to find the regions of the image with the significant possibility of representing human. Although this method will detect various non-natural objects, it is primarily intended and optimized for detection of humans; i.e. for search and rescue purposes in non-urban terrain where, in normal circumstances, non-natural objects shouldn-t be present. Real world images are used for the evaluation of the method.

Keywords: Landscape surveillance, mean shift algorithm, image segmentation, target detection.

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Authors: Badr M. Alshammari, A. Rabeh, A. K. Mohamed

Abstract:

This paper presents the modeling and the control of a grid-connected photovoltaic system (PVS). Firstly, the MPPT control of the PVS and its associated DC/DC converter has been analyzed in order to extract the maximum of available power. Secondly, the control system of the grid side converter (GSC) which is a three-phase voltage source inverter (VSI) has been presented. A special attention has been paid to the control algorithms of the GSC converter during grid voltages imbalances. Especially, three different control objectives are to achieve; the mitigation of the grid imbalance adverse effects, at the point of common coupling (PCC), on the injected currents, the elimination of double frequency oscillations in active power flow, and the elimination of double frequency oscillations in reactive power flow. Simulation results of two control strategies have been performed via MATLAB software in order to demonstrate the particularities of each control strategy according to power quality standards.

Keywords: Renewable energies, photovoltaic systems, DC link, voltage source inverter, space vector SVPWM, unbalanced voltage dips, symmetrical components.

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Authors: Sang-Ho Baik

Abstract:

D-erythro-cyclohexylserine (D chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on genomic sequence of Shinorhizobium meliloti Sequence analysis of the cloned DNA fragment revealed one open-reading frame of 1059 bp and 386 amino acids. This putative D-TA gene was cloned into NdeI and EcoRI (pEnsi His-tag sequence or BamHI (pEnsi-DTA[2]) sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with pEnsi-DTA[2]. When the cells expressing the wild used for D-TA enzyme activity, 12 mM glycine was successfully detected in HPLC analysis. Moreover, the whole cells harbouring the recombinant D-TA was able to synthesize D-erythro of 0.6 mg/ml in a batch reaction.

Keywords: About four key words or phrases in alphabetical order, separated by commas.

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