Search results for: flow cytometer
4576 Production Optimization through Ejector Installation at ESA Platform Offshore North West Java Field
Authors: Arii Bowo Yudhaprasetya, Ario Guritno, Agus Setiawan, Recky Tehupuring, Cosmas Supriatna
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The offshore facilities condition of Pertamina Hulu Energi Offshore North West Java (PHE ONWJ) varies greatly from place to place, depending on the characteristics of the presently installed facilities. In some locations, such as ESA platform, gas trap is mainly caused by the occurrence of flash gas phenomenon which is known as mechanical-physical separation process of multiphase flow. Consequently, the presence of gas trap at main oil line would accumulate on certain areas result in a reduced oil stream throughout the pipeline. Any presence of discrete gaseous along continuous oil flow represents a unique flow condition under certain specific volume fraction and velocity field. From gas lift source, a benefit line is used as a motive flow for ejector which is designed to generate a syphon effect to minimize the gas trap phenomenon. Therefore, the ejector’s exhaust stream will flow to the designated point without interfering other systems.Keywords: diffuser, ejector, flow, fluent
Procedia PDF Downloads 4344575 Variability in Saturation Flow and Traffic Performance at Urban Signalized Intersection
Authors: P. N. Salini, B. Anish Kini, R. Ashalatha
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At signalized intersections with heterogeneous traffic, the percentage share of different vehicle categories have a bearing on the inter-vehicle space utilization, which eventually impacts the saturation flow. This paper analyzed the impact of the percentage share of various vehicle categories in the traffic stream on the saturation flow at signalized intersections by video graphing major intersections with varying geometry in Kerala, India. It was found that as the percentage share of two-wheelers increases, the saturation flow at signalized intersections increases and vice-versa for the percentage share of cars. The effect of bus blockage and parking maneuvers on the saturation flow were also studied. As the distance of bus blockage increases from the stop line, the effect on the saturation flow decreases, while with more buses stopping at the same bus stop, the saturation flow reduces further. The study revealed that with higher kerbside parking maneuvers on the upstream, the saturation flow reduces, and with an increase in the distance of the parking maneuver from the stop line, the effect on the saturation flow decreases. The adjustment factors for bus blockage due to bus stops within 75m downstream and parking maneuvers within 75m upstream of the intersection have been established for mixed traffic conditions. These adjustment factors could empower the urban planners, enforcement personnel and decision-makers to estimate the reduction in the capacity of signalized intersections for suggesting improvements in the form of parking restrictions/ bus stop relocation for existing intersections or make design changes for planned intersections.Keywords: signalized intersection, saturation flow, adjustment factors, capacity
Procedia PDF Downloads 1244574 Experimental Investigation on the Effect of Cross Flow on Discharge Coefficient of an Orifice
Authors: Mathew Saxon A, Aneeh Rajan, Sajeev P
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Many fluid flow applications employ different types of orifices to control the flow rate or to reduce the pressure. Discharge coefficients generally vary from 0.6 to 0.95 depending on the type of the orifice. The tabulated value of discharge coefficients of various types of orifices available can be used in most common applications. The upstream and downstream flow condition of an orifice is hardly considered while choosing the discharge coefficient of an orifice. But literature shows that the discharge coefficient can be affected by the presence of cross flow. Cross flow is defined as the condition wherein; a fluid is injected nearly perpendicular to a flowing fluid. Most researchers have worked on water being injected into a cross-flow of water. The present work deals with water to gas systems in which water is injected in a normal direction into a flowing stream of gas. The test article used in the current work is called thermal regulator, which is used in a liquid rocket engine to reduce the temperature of hot gas tapped from the gas generator by injecting water into the hot gas so that a cooler gas can be supplied to the turbine. In a thermal regulator, water is injected through an orifice in a normal direction into the hot gas stream. But the injection orifice had been calibrated under backpressure by maintaining a stagnant gas medium at the downstream. The motivation of the present study aroused due to the observation of a lower Cd of the orifice in flight compared to the calibrated Cd. A systematic experimental investigation is carried out in this paper to study the effect of cross-flow on the discharge coefficient of an orifice in water to a gas system. The study reveals that there is an appreciable reduction in the discharge coefficient with cross flow compared to that without cross flow. It is found that the discharge coefficient greatly depends on the ratio of momentum of water injected to the momentum of the gas cross flow. The effective discharge coefficient of different orifices was normalized using the discharge coefficient without cross-flow and it is observed that normalized curves of effective discharge coefficient of different orifices with momentum ratio collapsing into a single curve. Further, an equation is formulated using the test data to predict the effective discharge coefficient with cross flow using the calibrated Cd value without cross flow.Keywords: cross flow, discharge coefficient, orifice, momentum ratio
Procedia PDF Downloads 1424573 Experimental and Computational Investigation of Flow Field and Thermal Behavior of a Mechanical Seal
Authors: Hossein Shokouhmand, Masoomeh Shadab, Rohallah Torabi
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Turbulent flow inside the seal chamber of a pump operating at nearly high Reynolds number is investigated. A comparison of a 3-D computational model for flow and thermal analysis of a mechanical seal with experimental thermal results is presented. The computational model adequately predicts the flow field in the seal chamber and thermal characteristics with the rotating and stationary rings and the twister flow around the seal parts by solving N-S and energy equations in ANSYS-CFX software. The Reynolds stress model (RSM) is applied as a turbulence model for this purpose. Experimental work is discussed which quantifies the temperature of five different points of the working fluid in chamber, mass flow at inlet and the fluid pressure at inlet and outlet. Experimental measurements are combined with computational modeling to obtain local and average heat transfer characteristics. Numerical results of three cases including different flush rates are reported.Keywords: mechanical seal, CFD_CFX, reynolds stress model, flow field, heat transfer analysis, stream line, heat transfer coefficient, heat flux, nusselt
Procedia PDF Downloads 4404572 Contribution to Experiments of a Free Surface Supercritical Flow over an Uneven Bottom
Authors: M. Bougamouza, M. Bouhadef, T. Zitoun
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The aim of this study is to examine, through experimentation in the laboratory, the supercritical flow in the presence of an obstacle in a rectangular channel. The supercritical regime in the whole hydraulic channel is achieved by adding a convergent. We will observe the influence of the obstacle shape and dimension on the characteristics of the supercritical flow, mainly the free-surface elevation and the velocity profile. The velocity measurements have been conducted with the one dimension laser anemometry technique.Keywords: experiments, free-surface flow, hydraulic channel, uneven bottom, laser anemometry, supercritical regime
Procedia PDF Downloads 2514571 An Experimental Study of the Influence of Flow Rate on Formation Damage at Different pH
Authors: Khabat M. Ahmad
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This experiment focuses on the reduction of permeability (formation damage) as a result of fines migration by changing pH and flow rate on core plugs selected from sandstone reservoir of Pannonian basin (Upper Miocene, East Hungary). The main objective of coreflooding experiments was to investigate the influence of both high and low pH fluids and the flow rate on stability of clay minerals. The selected core samples were examined by X-ray powder diffraction (XRD) for bulk mineralogical and clay mineral composition. The shape, position, distribution and type of clay minerals within the core samples were diagnosed by scanning electron microscopy and energy dispersive spectroscopy (SEM- EDS). The basic petrophysical properties such as porosity and initial permeability were determined prior to experiments. The special core analysis (influence of pH and flow rate) on permeability reduction was examined through a series of laboratory coreflooding experiments, testing for acidic (3) and alkaline (11) solutions at different flow rates (50, 100 and 200 ml/h). Permeability in continuously reduced for pH 11 to more than 50 % of initial permeability. However, at pH 3 after a slow decrease, a significant increase is observed, to more than 40 % of initial permeability. The variation is also influenced by flow rate.Keywords: flow rate, pH, permeability, fine migration, formation damage, XRD, SEM- EDS
Procedia PDF Downloads 604570 Blood Flow in Stenosed Arteries: Analytical and Numerical Study
Authors: Shashi Sharma, Uaday Singh, V. K. Katiyar
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Blood flow through a stenosed tube, which is of great interest to mechanical engineers as well as medical researchers. If stenosis exists in an artery, normal blood flow is disturbed. The deposition of fatty substances, cholesterol, cellular waste products in the inner lining of an artery results to plaque formation .The present study deals with a mathematical model for blood flow in constricted arteries. Blood is considered as a Newtonian, incompressible, unsteady and laminar fluid flowing in a cylindrical rigid tube along the axial direction. A time varying pressure gradient is applied in the axial direction. An analytical solution is obtained using the numerical inversion method for Laplace Transform for calculating the velocity profile of fluid as well as particles.Keywords: blood flow, stenosis, Newtonian fluid, medical biology and genetics
Procedia PDF Downloads 5164569 Effect of Radiation on Magnetohydrodynamic Two Phase Stenosed Arterial Blood Flow with Heat and Mass Transfer
Authors: Bhavya Tripathi, Bhupendra Kumar Sharma
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In blood, the concentration of red blood cell varies with the arterial diameter. In the case of narrow arteries, red blood cells concentrate around the center of the artery and there exists a cell-free plasma layer near the arterial wall due to Fahraeus-Lindqvist effect. Due to non- uniformity of the fluid in the narrow arteries, it is preferable to consider the two-phase model of the blood flow. In the present article, coupled nonlinear differential equations have been developed for momentum, energy and concentration of two phase model of the blood flow assuming the Newtonian fluid in both central core and cell free plasma layer and the exact solutions have been found for the problem. For having an adequate insight into the stenosed arterial two-phase blood flow, major components of the flow as flow resistance, total flow rate, and wall shear stress have been estimated for different values of magnetic and radiation parameter. Results show that the increase in the effects of magnetic field decreases the velocity of both cores as well as plasma regions. This result can be helpful to control the blood flow in narrow arteries during surgical process. Temperature of core as well plasma regions decrease as value of radiation parameter increases. The present result is implemented in the form of radiation therapy which is very helpful for cancer patients.Keywords: two phase blood flow, radiation, magnetohydrodynamics (MHD), stenosis
Procedia PDF Downloads 2054568 Flow Field Analysis of a Liquid Ejector Pump Using Embedded Large Eddy Simulation Methodology
Authors: Qasim Zaheer, Jehanzeb Masud
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The understanding of entrainment and mixing phenomenon in the ejector pump is of pivotal importance for designing and performance estimation. In this paper, the existence of turbulent vortical structures due to Kelvin-Helmholtz instability at the free surface between the motive and the entrained fluids streams are simulated using Embedded LES methodology. The efficacy of Embedded LES for simulation of complex flow field of ejector pump is evaluated using ANSYS Fluent®. The enhanced mixing and entrainment process due to breaking down of larger eddies into smaller ones as a consequence of Vortex Stretching phenomenon is captured in this study. Moreover, the flow field characteristics of ejector pump like pressure velocity fields and mass flow rates are analyzed and validated against the experimental results.Keywords: Kelvin Helmholtz instability, embedded LES, complex flow field, ejector pump
Procedia PDF Downloads 2974567 Evaluation of Sloshing in Process Equipment for Floating Cryogenic Application
Authors: Bo Jin
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A variety of process equipment having flow in and out is widely used in industrial land-based cryogenic facilities. In some of this equipment, such as vapor-liquid separator, a liquid level is established during the steady operation. As the implementation of such industrial processes extends to off-shore floating facilities, it is important to investigate the effect of sea motion on the process equipment partially filled with liquid. One important aspect to consider is the occurrence of sloshing therein. The flow characteristics are different from the classical study of sloshing, where the fluid is enclosed inside a vessel (e.g., storage tank) with no flow in or out. Liquid inside process equipment continuously flows in and out of the system. To understand this key difference, a Computational Fluid Dynamics (CFD) model is developed to simulate the liquid motion inside a partially filled cylinder with and without continuous flow in and out. For a partially filled vertical cylinder without any continuous flow in and out, the CFD model is found to be able to capture the well-known sloshing behavior documented in the literature. For the cylinder with a continuous steady flow in and out, the CFD simulation results demonstrate that the continuous flow suppresses sloshing. Given typical cryogenic fluid has very low viscosity, an analysis based on potential flow theory is developed to explain why flow into and out of the cylinder changes the natural frequency of the system and thereby suppresses sloshing. This analysis further validates the CFD results.Keywords: computational fluid dynamics, CFD, cryogenic process equipment, off-shore floating processes, sloshing
Procedia PDF Downloads 1374566 The Effect of Bottom Shape and Baffle Length on the Flow Field in Stirred Tanks in Turbulent and Transitional Flow
Authors: Jie Dong, Binjie Hu, Andrzej W Pacek, Xiaogang Yang, Nicholas J. Miles
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The effect of the shape of the vessel bottom and the length of baffles on the velocity distributions in a turbulent and in a transitional flow has been simulated. The turbulent flow was simulated using standard k-ε model and simulation was verified using LES whereas transitional flow was simulated using only LES. It has been found that both the shape of tank bottom and the baffles’ length has significant effect on the flow pattern and velocity distribution below the impeller. In the dished bottom tank with baffles reaching the edge of the dish, the large rotating volume of liquid was formed below the impeller. Liquid in this rotating region was not fully mixing. A dead zone was formed here. The size and the intensity of circulation within this zone calculated by k-ε model and LES were practically identical what reinforces the accuracy of the numerical simulations. Both types of simulations also show that employing full-length baffles can reduce the size of dead zone formed below the impeller. The LES was also used to simulate the velocity distribution below the impeller in transitional flow and it has been found that secondary circulation loops were formed near the tank bottom in all investigated geometries. However, in this case the length of baffles has smaller effect on the volume of rotating liquid than in the turbulent flow.Keywords: baffles length, dished bottom, dead zone, flow field
Procedia PDF Downloads 2954565 A Novel NRIS Index to Evaluate Brain Activity in Prefrontal Regions While Listening to First and Second Languages for Long Time Periods
Authors: Kensho Takahashi, Ko Watanabe, Takashi Kaburagi, Hiroshi Tanaka, Kajiro Watanabe, Yosuke Kurihara
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Near-infrared spectroscopy (NIRS) has been widely used as a non-invasive method to measure brain activity, but it is corrupted by baseline drift noise. Here we present a method to measure regional cerebral blood flow as a derivative of NIRS output. We investigate whether, when listening to languages, blood flow can reasonably localize and represent regional brain activity or not. The prefrontal blood flow distribution pattern when advanced second-language listeners listened to a second language (L2) was most similar to that when listening to their first language (L1) among the patterns of mean and standard deviation. In experiments with 25 healthy subjects, the maximum blood flow was localized to the left BA46 of advanced listeners. The blood flow presented is robust to baseline drift and stably localizes regional brain activity.Keywords: NIRS, oxy-hemoglobin, baseline drift, blood flow, working memory, BA46, first language, second language
Procedia PDF Downloads 5584564 Secured Power flow Algorithm Including Economic Dispatch with GSDF Matrix Using LabVIEW
Authors: Slimane Souag, Amel Graa, Farid Benhamida
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In this paper we present a new method for solving the secured power flow problem by the economic dispatch using DC power flow method and Generation Shift Distribution Factor (GSDF), in this work we create a graphical interface in LabVIEW as a virtual instrument. Hence the dc power flow reduces the power flow problem to a set of linear equations, which make the iterative calculation very fast and the GSFD matrix present the effects of single and multiple generator MW change on the transmission line. The effectiveness of the method developed is identified through its application to an IEEE-14 bus test system. The calculation results show excellent performance of the proposed method, in regard to computation time and quality of results.Keywords: electrical power system security, economic dispatch, sensitivity matrix, labview
Procedia PDF Downloads 4894563 Gas Flow, Time, Distance Dynamic Modelling
Authors: A. Abdul-Ameer
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The equations governing the distance, pressure- volume flow relationships for the pipeline transportation of gaseous mixtures, are considered. A derivation based on differential calculus, for an element of this system model, is addressed. Solutions, yielding the input- output response following pressure changes, are reviewed. The technical problems associated with these analytical results are identified. Procedures resolving these difficulties providing thereby an attractive, simple, analysis route are outlined. Computed responses, validating thereby calculated predictions, are presented.Keywords: pressure, distance, flow, dissipation, models
Procedia PDF Downloads 4734562 Computational Fluid Dynamics Modeling of Flow Properties Fluctuations in Slug-Churn Flow through Pipe Elbow
Authors: Nkemjika Chinenye-Kanu, Mamdud Hossain, Ghazi Droubi
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Prediction of multiphase flow induced forces, void fraction and pressure is crucial at both design and operating stages of practical energy and process pipe systems. In this study, transient numerical simulations of upward slug-churn flow through a vertical 90-degree elbow have been conducted. The volume of fluid (VOF) method was used to model the two-phase flows while the K-epsilon Reynolds-Averaged Navier-Stokes (RANS) equations were used to model turbulence in the flows. The simulation results were validated using experimental results. Void fraction signal, peak frequency and maximum magnitude of void fraction fluctuation of the slug-churn flow validation case studies compared well with experimental results. The x and y direction force fluctuation signals at the elbow control volume were obtained by carrying out force balance calculations using the directly extracted time domain signals of flow properties through the control volume in the numerical simulation. The computed force signal compared well with experiment for the slug and churn flow validation case studies. Hence, the present numerical simulation technique was able to predict the behaviours of the one-way flow induced forces and void fraction fluctuations.Keywords: computational fluid dynamics, flow induced vibration, slug-churn flow, void fraction and force fluctuation
Procedia PDF Downloads 1564561 Thermal Performance Investigation on Cross V-Shape Solar Air Collectors
Authors: Xi Luo, Xu Ji, Yunfeng Wang, Guoliang Li, Chongqiang Yan, Ming Li
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Two different kinds of cross V-shape solar air collectors are designed and constructed. In the transverse cross V-shape collector, the V-shape bottom plate is along the air flow direction and the absorbing plate is perpendicular to the air flow direction. In the lengthway cross V-shape collector, the V-shape absorbing plate is along the air flow direction and the bottom plate is perpendicular to the air flow direction. Based on heat balance, the mathematical model is built to evaluate their performances. These thermal performances of the two cross V-shape solar air collectors and an extra traditional flat-plate solar air collector are characterized under various operating conditions by experiments. The experimental results agree well with the calculation values. The experimental results prove that the thermal efficiency of transverse cross V-shape collector precedes that of others. The air temperature at any point along the flow direction of the transverse cross V-shape collector is higher than that of the lengthway cross V-shape collector. For the transverse cross V-shape collector, the most effective length of flow channel is 0.9m. For the lengthway cross V-shape collector, a longer flow channel is necessary to achieve a good thermal performance.Keywords: cross v-shape, performance, solar air collector, thermal efficiency
Procedia PDF Downloads 3114560 Chemical Fingerprinting of Complex Samples With the Aid of Parallel Outlet Flow Chromatography
Authors: Xavier A. Conlan
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Speed of analysis is a significant limitation to current high-performance liquid chromatography/mass spectrometry (HPLC/MS) and ultra-high-pressure liquid chromatography (UHPLC)/MS systems both of which are used in many forensic investigations. The flow rate limitations of MS detection require a compromise in the chromatographic flow rate, which in turn reduces throughput, and when using modern columns, a reduction in separation efficiency. Commonly, this restriction is combated through the post-column splitting of flow prior to entry into the mass spectrometer. However, this results in a loss of sensitivity and a loss in efficiency due to the post-extra column dead volume. A new chromatographic column format known as 'parallel segmented flow' involves the splitting of eluent flow within the column outlet end fitting, and in this study we present its application in order to interrogate the provenience of methamphetamine samples with mass spectrometry detection. Using parallel segmented flow, column flow rates as high as 3 mL/min were employed in the analysis of amino acids without post-column splitting to the mass spectrometer. Furthermore, when parallel segmented flow chromatography columns were employed, the sensitivity was more than twice that of conventional systems with post-column splitting when the same volume of mobile phase was passed through the detector. These finding suggest that this type of column technology will particularly enhance the capabilities of modern LC/MS enabling both high-throughput and sensitive mass spectral detection.Keywords: chromatography, mass spectrometry methamphetamine, parallel segmented outlet flow column, forensic sciences
Procedia PDF Downloads 4894559 Measurement of Liquid Film Thickness in a Vertical Annular Two Phase Flow Changing the Gas-Liquid Density Ratio
Authors: Shoji Mori, Kunito Okuyama
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Annular two phase flow is encountered in many industrial equipments, including flow near nuclear fuel rods in boiling water reactor (BWR). Especially, disturbance waves play important roles in the pressure drop, the generation of entrainments, and the dryout of the liquid film. Therefore, it is important to clarify the behavior of disturbance waves and base film. However, most of the previous studies have been performed under atmospheric pressure conditions that provides the properties of liquid and gas which are significantly different from those of a BWR. Therefore, the effect of properties in gas and liquid on liquid film characteristics should be clarified. In this paper we focus on the effect of gas-liquid density ratio on liquid film thickness characteristics. The experiments have been conducted at four density ratio conditions (ρL/ρG =763, 451, 231, and 31). As a result, it is found that and interfacial shear stress collapse not only tF ave but also tF max and tF min successfully under the same liquid mass flow rate conditions irrespective of ρL/ρG, and moreover a non-dimensional parameter tends to collapse tF max,tF ave,and tF min in the wide range of experimental conditions (ρL/ρG:31~763,We:10~1800,ReL:500 ~ 2200).Keywords: two phase flow, liquid film, annular flow, disturbance wave
Procedia PDF Downloads 3894558 Experimental Study of the Modifications of the Bed of a River under Extreme Flow Conditions
Authors: A. Ghenaim, A. Terfous
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In this work, degradation phenomena in fluvial beds having uniform sediments are explored experimentally under extreme flow conditions. Laboratory experiments were conducted in a rectangular cross-section channel for different flow conditions, channel characteristics, and sediment properties at the National Institute of Applied Sciences (Strasbourg, France). Tests were carried out in two conditions: (1) equilibrium condition, where, once the steady and uniform flow conditions were achieved for a given slope and discharge, the channel was fed with variable sediment discharges until the bed-load sediment transport achieved an equilibrium condition; and (2) nonequilibrium condition, where the sediment feeding was instantaneously stopped, and the bed levels were measured over time. Experimental results enabled assessing the erosion rates and determining the empirical mathematical model to predict the bed level changes.Keywords: fluvial beds, sediment, uniform flow conditions, nonequilibrium condition, sediment disposition, erosion
Procedia PDF Downloads 944557 Modeling of Full Range Flow Boiling Phenomenon in 23m Long Vertical Steam Generator Tube
Authors: Chaitanya R. Mali, V. Vinod, Ashwin W. Patwardhan
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Design of long vertical steam generator (SG) tubes in nuclear power plant involves an understanding of different aspects of flow boiling phenomenon such as flow instabilities, flow regimes, dry out, critical heat flux, pressure drop, etc. The knowledge of the prediction of local thermal hydraulic characteristics is necessary to understand these aspects. For this purpose, the methodology has been developed which covers all the flow boiling regimes to model full range flow boiling phenomenon. In this methodology, the vertical tube is divided into four sections based on vapor fraction value at the end of each section. Different modeling strategies have been applied to the different sections of the vertical tube. Computational fluid dynamics simulations have been performed on a vertical SG tube of 0.0126 m inner diameter and 23 m length. The thermal hydraulic parameters such as vapor fraction, liquid temperature, heat transfer coefficient, pressure drop, heat flux distribution have been analyzed for different designed heat duties (1.1 MW (20%) to 3.3 MW (60%)) and flow conditions (10 % to 80 %). The sensitivity of different boiling parameters such as bubble departure diameter, nucleation site density, bubble departure frequency on the thermal hydraulic parameters was also studied. Flow instability has been observed at 20 % designed heat duty and 20 % flow conditions.Keywords: thermal hydraulics, boiling, vapor fraction, sensitivity
Procedia PDF Downloads 1474556 Study of the Behavior and Similarities of Flow and Level Transmitters in the Industries
Authors: Valentina Alessandra Carvalho do Vale, Elmo Thiago Lins Cöuras Ford
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In view of the requirements of the current industrial processes, the instrumentation plays a critical role. In this context, this work aims to raise some the operating characteristics of the level and flow transmitters, in addition to observing their similarities and possible limitations for certain configurations.Keywords: flow, level, instrumentation, configurations of meters, method of choice of the meters, instrumentation in the industrial processes
Procedia PDF Downloads 5784555 Role of Autophagic Lysosome Reformation for Cell Viability in an in vitro Infection Model
Authors: Muhammad Awais Afzal, Lorena Tuchscherr De Hauschopp, Christian Hübner
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Introduction: Autophagy is an evolutionarily conserved lysosome-dependent degradation pathway, which can be induced by extrinsic and intrinsic stressors in living systems to adapt to fluctuating environmental conditions. In the context of inflammatory stress, autophagy contributes to the elimination of invading pathogens, the regulation of innate and adaptive immune mechanisms, and regulation of inflammasome activity as well as tissue damage repair. Lysosomes can be recycled from autolysosomes by the process of autophagic lysosome reformation (ALR), which depends on the presence of several proteins including Spatacsin. Thus ALR contributes to the replenishment of lysosomes that are available for fusion with autophagosomes in situations of increased autophagic turnover, e.g., during bacterial infections, inflammatory stress or sepsis. Objectives: We aimed to assess whether ALR plays a role for cell survival in an in-vitro bacterial infection model. Methods: Mouse embryonic fibroblasts (MEFs) were isolated from wild-type mice and Spatacsin (Spg11-/-) knockout mice. Wild-type MEFs and Spg11-/- MEFs were infected with Staphylococcus aureus (multiplication of infection (MOI) used was 10). After 8 and 16 hours of infection, cell viability was assessed on BD flow cytometer through propidium iodide intake. Bacterial intake by cells was also calculated by plating cell lysates on blood agar plates. Results: in-vitro infection of MEFs with Staphylococcus aureus showed a marked decrease of cell viability in ALR deficient Spatacsin knockout (Spg11-/-) MEFs after 16 hours of infection as compared to wild-type MEFs (n=3 independent experiments; p < 0.0001) although no difference was observed for bacterial intake by both genotypes. Conclusion: Suggesting that ALR is important for the defense of invading pathogens e.g. S. aureus, we observed a marked increase of cell death in an in-vitro infection model in cells with compromised ALR.Keywords: autophagy, autophagic lysosome reformation, bacterial infections, Staphylococcus aureus
Procedia PDF Downloads 1444554 Inducing Flow Experience in Mobile Learning: An Experiment Using a Spanish Learning Mobile Application
Authors: S. Jonsson, D. Millard, C. Bokhove
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Smartphones are ubiquitous and frequently used as learning tools, which makes the design of educational apps an important area of research. A key issue is designing apps to encourage engagement while maintaining a focus on the educational aspects of the app. Flow experience is a promising method for addressing this issue, which refers to a mental state of cognitive absorption and positive emotion. Flow experience has been shown to be associated with positive emotion and increased learning performance. Studies have shown that immediate feedback is an antecedent to Flow. This experiment investigates the effect of immediate feedback on Flow experience. An app teaching Spanish phrases was developed, and 30 participants completed both a 10min session with immediate feedback and a 10min session with delayed feedback. The app contained a task where the user assembles Spanish phrases by pressing bricks with Spanish words. Immediate feedback was implemented by incorrect bricks recoiling, while correct brick moved to form part of the finished phrase. In the delayed feedback condition, the user did not know if the bricks they pressed were correct until the phrase was complete. The level of Flow experienced by the participants was measured after each session using the Flow Short Scale. The results showed that higher levels of Flow were experienced in the immediate feedback session. It was also found that 14 of the participants indicated that the demands of the task were ‘just right’ in the immediate feedback session, while only one did in the delayed feedback session. These results have implications for how to design educational technology and opens up questions for how Flow experience can be used to increase performance and engagement.Keywords: feedback timing, flow experience, L2 language learning, mobile learning
Procedia PDF Downloads 1314553 Flow Control Optimisation Using Vortex Generators in Turbine Blade
Authors: J. Karthik, G. Vinayagamurthy
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Aerodynamic flow control is achieved by interaction of flowing medium with corresponding structure so that its natural flow state is disturbed to delay the transition point. This paper explains the aerodynamic effect and optimized design of Vortex Generators on the turbine blade to achieve maximum flow control. The airfoil is chosen from NREL [National Renewable Energy Laboratory] S-series airfoil as they are characterized with good lift characteristics and lower noise. Vortex generators typically chosen are Ogival, Rectangular, Triangular and Tapered Fin shapes attached near leading edge. Vortex generators are typically distributed from the primary to tip of the blade section. The design wind speed is taken as 6m/s and the computational analysis is executed. The blade surface is simulated using k- ɛ SST model and results are compared with X-FOIL results. The computational results are validated using Wind Tunnel Testing of the blade corresponding to the design speed. The effect of Vortex generators on the flow characteristics is studied from the results of analysis. By comparing the computational and test results of all shapes of Vortex generators; the optimized design is achieved for effective flow control corresponding to the blade.Keywords: flow control, vortex generators, design optimisation, CFD
Procedia PDF Downloads 4084552 Investigation of the Kutta Condition Using Unsteady Flow
Authors: K. Bhojnadh, M. Fiddler, D. Cheshire
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An investigation into the Kutta effect on the trailing edge of a subsonic aerofoil was conducted which led to an analysis using Ansys Fluent to determine the effect of flow separation over a NACA 0012 aerofoil. This aerofoil was subjected to oscillations to create an unsteady flow over the aerofoil, therefore, creating turbulence, with unsteady aerodynamics playing a key role to determine the flow regimes when the aerofoil is subjected to different angles of attack along with varying Reynolds numbers. Many theories were evolved to determine the flow parameters of a 2-D aerofoil in these unsteady conditions because they behave unpredictably at the trailing edge when subjected to a different angle of attack. The shear area observed in the boundary layer at the trailing edge tends towards an unsteady turbulent flow even at small angles of attack, creating drag as the flow separates, reducing the aerodynamic performance of aerofoil. In this paper, research was conducted to determine the effect of Kutta circulation over the aerofoil and the effect of that circulation in reducing the effect of pressure and boundary layer distribution over the aerofoil. The effect of circulation is observed by using Ansys Fluent by using varying flow parameters and differential schemes to observe the flow behaviour on the aerofoil. Initially, steady flow analysis was conducted on the aerofoil to determine the effect of circulation, and it was noticed that the effect of circulation could only be properly observed when the aerofoil is subjected to oscillations. Therefore, that was modelled by using Ansys user-defined functions, which define the motion of the aerofoil by creating a dynamic mesh on the aerofoil. Initial results were observed, and further development of the dynamic mesh functions in Ansys is taking place. This research will determine the overall basic principles of unsteady flow aerodynamics applied to the investigation of Kutta related circulation, and gives an indication regarding the generation of vortices which is discussed further in this paper.Keywords: circulation, flow seperation, turbulence modelling, vortices
Procedia PDF Downloads 2054551 Convective Brinkman-Forchiemer Extended Flow through Channel Filled with Porous Material: An Approximate Analytical Approach
Authors: Basant K. Jha, M. L. Kaurangini
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An approximate analytical solution is presented for convective flow in a horizontal channel filled with porous material. The Brinkman-Forchheimer extension of Darcy equation is utilized to model the fluid flow while the energy equation is utilized to model temperature distribution in the channel. The solutions were obtained utilizing the newly suggested technique and compared with those obtained from an implicit finite-difference solution.Keywords: approximate analytical, convective flow, porous material, Brinkman-Forchiemer
Procedia PDF Downloads 3954550 Numerical Simulation of Erosion Control in Slurry Pump Casing by Geometrical Flow Pattern Modification Analysis
Authors: A. R. Momeninezhad
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Erosion of Slurry Pumps in Related Industries, is one of the major costs in their production process. Many factories in extractive industries try to find ways to diminish this cost. In this paper, we consider the flow pattern modifications by geometric variations made of numerical simulation of flow inside pump casing, which is one of the most important parts analyzed for erosion. The mentioned pump is a cyclone centrifugal slurry pump, which is operating in Sarcheshmeh Copper Industries in Kerman-Iran, named and tagged as HM600 cyclone pump. Simulation shows many improvements in local wear information and situations for better and more qualified design of casing shape and impeller position, before and after geometric corrections. By theory of liquid-solid two-phase flow, the local wear defeats are analyzed and omitted.Keywords: flow pattern, slurry pump, simulation, wear
Procedia PDF Downloads 4574549 The Phenomena of Virtual World Adoption: Antecedents and Consequences of Virtual World Experience
Authors: Norita Ahmad, Reza Barkhi, Xiaobo Xu
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We design an experimental study to learn about the cognitive implications of the use of avatars in a Virtual World (VW) (i.e., Second Life). The results support our proposed model, where a positive flow experience with VW influences the attitude towards VW, in turn influencing intention to use VW. Furthermore, VW flow experience can itself be impacted by perceived peer influence, familiarity with VW, and personality of the individuals behind the avatars in VW.Keywords: avatar, flow experience, personality type, second life, virtual world
Procedia PDF Downloads 5974548 Pulsed Vortex Flow in Low–Temperature Range Heat Pipes
Authors: A. V. Seryakov
Abstract:
The work presents part calculation and part experimental research of the intensification of heat-transfer characteristics of medium-temperature heat pipes. Presented is a vapour jet nozzle, similar to the Laval nozzle, surrounded by a capillary-porous insert along the full length of the heat pipe axial to the direction of heat flow. This increases velocity of the vapour flow, heat-transfer coefficient and pulse rate of two-phase vapour flow.Keywords: medium-temperature range heat pipes, capillary-porous insert, capillary steam injectors, Laval nozzle, condensation sensor
Procedia PDF Downloads 4374547 Numerical Study on Parallel Rear-Spoiler on Super Cars
Authors: Anshul Ashu
Abstract:
Computers are applied to the vehicle aerodynamics in two ways. One of two is Computational Fluid Dynamics (CFD) and other is Computer Aided Flow Visualization (CAFV). Out of two CFD is chosen because it shows the result with computer graphics. The simulation of flow field around the vehicle is one of the important CFD applications. The flow field can be solved numerically using panel methods, k-ε method, and direct simulation methods. The spoiler is the tool in vehicle aerodynamics used to minimize unfavorable aerodynamic effects around the vehicle and the parallel spoiler is set of two spoilers which are designed in such a manner that it could effectively reduce the drag. In this study, the standard k-ε model of the simplified version of Bugatti Veyron, Audi R8 and Porsche 911 are used to simulate the external flow field. Flow simulation is done for variable Reynolds number. The flow simulation consists of three different levels, first over the model without a rear spoiler, second for over model with single rear spoiler, and third over the model with parallel rear-spoiler. The second and third level has following parameter: the shape of the spoiler, the angle of attack and attachment position. A thorough analysis of simulations results has been found. And a new parallel spoiler is designed. It shows a little improvement in vehicle aerodynamics with a decrease in vehicle aerodynamic drag and lift. Hence, it leads to good fuel economy and traction force of the model.Keywords: drag, lift, flow simulation, spoiler
Procedia PDF Downloads 500