Search results for: sulfuric acid attacks.

Authors: Zied Trifa, Maher Khemakhem

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The survey and classification of the different security attacks in structured peer-to-peer (P2P) overlay networks can be useful to computer system designers, programmers, administrators, and users. In this paper, we attempt to provide a taxonomy of structured P2P overlay networks security attacks. We have specially focused on the way these attacks can arise at each level of the network. Moreover, we observed that most of the existing systems such as Content Addressable Network (CAN), Chord, Pastry, Tapestry, Kademlia, and Viceroy suffer from threats and vulnerability which lead to disrupt and corrupt their functioning. We hope that our survey constitutes a good help for who-s working on this area of research.

Keywords: P2P, Structured P2P Overlay Networks, DHT, Security, classification

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Authors: Byung-Ik Kim, Hong-Koo Kang, Tae-Jin Lee, Hae-Ryong Park

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Cyber terrors against specific enterprises or countries have been increasing recently. Such attacks against specific targets are called advanced persistent threat (APT), and they are giving rise to serious social problems. The malicious behaviors of APT attacks mostly affect websites and penetrate enterprise networks to perform malevolent acts. Although many enterprises invest heavily in security to defend against such APT threats, they recognize the APT attacks only after the latter are already in action. This paper discusses the characteristics of APT attacks at each step as well as the strengths and weaknesses of existing malicious code detection technologies to check their suitability for detecting APT attacks. It then proposes a network-based malicious behavior detection algorithm to protect the enterprise or national networks.

Keywords: Advanced Persistent Threat, Malware, Network Security, Network Packet, Exploit Kits.

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Authors: Jhalique Jane R. Fojas, Ernesto J. Del Rosario

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The dilute acid pretreatment and enzymatic saccharification of lignocellulosic substrate, cogon grass (Imperata cylindrical, L.) was optimized prior ethanol fermentation using simultaneous saccharification and fermentation (SSF) method. The optimum pretreatment conditions, temperature, sulfuric acid concentration, and reaction time were evaluated by determining the maximum sugar yield at constant enzyme loading. Cogon grass, at 10% w/v substrate loading, has optimum pretreatment conditions of 126°C, 0.6% v/v H2SO4, and 20min reaction time. These pretreatment conditions were used to optimize enzymatic saccharification using different enzyme combinations. The maximum saccharification yield of 36.68mg/mL (71.29% reducing sugar) was obtained using 25FPU/g-cellulose cellulase complex combined with 1.1% w/w of cellobiase, ß-glucosidase, and 0.225% w/w of hemicellulase complex, after 96 hours of saccharification. Using the optimum pretreatment and saccharification conditions, SSF of treated substrates was done at 37°C for 120 hours using industrial yeast strain HBY3, Saccharomyces cerevisiae. The ethanol yield for cogon grass at 4% w/w loading was 9.11g/L with 5.74mg/mL total residual sugar.

Keywords: Acid pretreatment, bioethanol, biomass, cogon grass, fermentation, lignocellylose, SSF.

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Authors: Tun Myat Aung, Ni Ni Hla

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This paper begins by describing basic properties of finite field and elliptic curve cryptography over prime field and binary field. Then we discuss the discrete logarithm problem for elliptic curves and its properties. We study the general common attacks on elliptic curve discrete logarithm problem such as the Baby Step, Giant Step method, Pollard’s rho method and Pohlig-Hellman method, and describe in detail experiments of these attacks over prime field and binary field. The paper finishes by describing expected running time of the attacks and suggesting strong elliptic curves that are not susceptible to these attacks.c

Keywords: Discrete logarithm problem, general attacks, elliptic curves, strong curves, prime field, binary field, attack experiments.

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Authors: Semuel Unwakoly, Reinner Puppela, Maresthy Rumalean, Healthy Kainama

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Fish is a kind of food that contains many nutritions, one of those is the long chain of unsaturated fatty acids as omega-3 and omega-6 fatty acids and essential amino acid in enough amount for the necessity of our body. Like pelagic fish that found in the sea of Maluku. This research was done to identify fatty acids and amino acids composition in Moonfish (M. maculata) using transesterification reaction steps and Gas Chromatograph-Mass Spectrophotometer (GC-MS) and High-Performance Liquid Chromatography (HPLC). The result showed that fatty acids composition in Moonfish (M. maculata) contained tridecanoic acid (2.84%); palmitoleic acid (2.65%); palmitic acid (35.24%); oleic acid (6.2%); stearic acid (14.20%); and 5,8,11,14-eicosatetraenoic acid (1.29%) and 12 amino acids composition that consist of 7 essential amino acids, were leucine, isoleucine, valine, phenylalanine, methionine, lysine, and histidine, and also 5 non-essential amino acid, were tyrosine, glycine, alanine, glutamic acid, and arginine.Thus, these fishes can be used by the people to complete the necessity of essential fatty acid and amino acid.

Keywords: Moonfish (M. maculata), fatty acid, amino acid, GC-MS, HPLC.

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Authors: Muhammad R Ahmed, Xu Huang, Dharmendra Sharma

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Developments in communication technologies especially in wireless have enabled the progress of low-cost and lowpower wireless sensor networks (WSNs). The features of such WSN are holding minimal energy, weak computational capabilities, wireless communication and an open-medium nature where sensors are deployed. WSN is underpinned by application driven such as military applications, the health sector, etc. Due to the intrinsic nature of the network and application scenario, WSNs are vulnerable to many attacks externally and internally. In this paper we have focused on the types of internal attacks of WSNs based on OSI model and discussed some security requirements, characterizers and challenges of WSNs, by which to contribute to the WSN-s security research.

Keywords: Wireless sensor network, internal attacks, security, OSI model.

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Authors: Elham Moniri, Homayon Ahmad Panahi, Ahmad Izadi, Mohamad Mehdi Parvin, Atyeh Rahimi

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In this investigation Salicylic acid, Sulfosalicylic acid and Acetyl salicylic acid were chosen as a sample for thin layer chromatography (TLC) on silica gel plates. Bicarbonate buffer at different pH containing different amounts of boric acid was applied as mobile phase. Specific interaction of these substances with boric acid has effect on Rf in thin layer chromatography. Regular and similar trend was observed in variations of Rf for mentioned compounds in TLC by altering of percentages of boric acid in mobile phase in pH range of 8-10. Also effect of organic solvent, mixture of water/ organic solvent and organic solvent containing boric acid as mobile phase was studied.

Keywords: Thin layer chromatography (TLC), Aspirin, Salicylic acid, Sulfosalycylic acid, Boric acid.

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Authors: Ahmed Abdulla Ashlam, Atta Badii, Frederic Stahl

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In recent years, SQL injection attacks have been identified as being prevalent against web applications. They affect network security and user data, which leads to a considerable loss of money and data every year. This paper presents the use of classification algorithms in machine learning using a method to classify the login data filtering inputs into "SQLi" or "Non-SQLi,” thus increasing the reliability and accuracy of results in terms of deciding whether an operation is an attack or a valid operation. A method as a Web-App is developed for auto-generated data replication to provide a twin of the targeted data structure. Shielding against SQLi attacks (WebAppShield) that verifies all users and prevents attackers (SQLi attacks) from entering and or accessing the database, which the machine learning module predicts as "Non-SQLi", has been developed. A special login form has been developed with a special instance of the data validation; this verification process secures the web application from its early stages. The system has been tested and validated, and up to 99% of SQLi attacks have been prevented.

Keywords: SQL injection, attacks, web application, accuracy, database, WebAppShield.

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Authors: Abdul Rahim Yacob, Mohamad Raizul Zinalibdin

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A simple method for the simultaneous determination of hippuric acid and benzoic acid in urine using reversed-phase high performance liquid chromatography was described. Chromatography was performed on a Nova-Pak C18 (3.9 x 150 mm) column with a mobile phase of mixed solution methanol: water: acetic acid (20:80:0.2) and UV detection at 254 nm. The calibration curve was linear within concentration range at 0.125 to 6.0 mg/ml of hippuric acid and benzoic acid. The recovery, accuracy and coefficient variance of hippuric acid were 104.54%, 0.2% and 0.2% respectively and for benzoic acid were 98.48%, 1.25% and 0.60% respectively. The detection limit of this method was 0.01ng/l for hippuric acid and 0.06ng/l for benzoic acid. This method has been applied to the analysis of urine samples from the suspected of toluene abuser or glue sniffer among secondary school students at Johor Bahru.

Keywords: Glue sniffer, High Performance LiquidChromatography, Hippuric Acid, Toluene, Urine.

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Authors: Jeong-Tae Kim, Tchanghee Hyun

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The number of intrusions and attacks against critical infrastructures and other information networks is increasing rapidly. While there is no identified evidence that terrorist organizations are currently planning a coordinated attack against the vulnerabilities of computer systems and network connected to critical infrastructure, and origins of the indiscriminate cyber attacks that infect computers on network remain largely unknown. The growing trend toward the use of more automated and menacing attack tools has also overwhelmed some of the current methodologies used for tracking cyber attacks. There is an ample possibility that this kind of cyber attacks can be transform to cyberterrorism caused by illegal purposes. Cyberterrorism is a matter of vital importance to national welfare. Therefore, each countries and organizations have to take a proper measure to meet the situation and consider effective legislation about cyberterrorism.

Keywords: Cyberterrorism, cyber attack, information security, legislation

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Authors: Jamal Wiwoho, Pujiyono, Triyanto

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Terrorism is a real enemy for all countries, including Indonesia. Bomb attacks in some parts of Indonesia are proof that Indonesia has serious problems with terrorism. Perpetrators of terror are arrested and imprisoned, and some of them were executed. However, this method did not succeed in stopping the terrorist attacks. Former terrorists continue to carry out bomb attacks. Therefore, this paper proposes a program towards deradicalization efforts of former terrorists through entrepreneurship. This is necessary because it is impossible to change their radical ideology. The program is also motivated by understanding that terrorists generally come from poor families. This program aims to occupy their time with business activities so there is no time to plan and carry out bomb attacks. This research is an empirical law study. Data were collected by literature study, observation, and in-depth interviews. Data were analyzed with the Miles and Huberman interactive model. The results show that the entrepreneurship program is effective to prevent terrorist attack. Former terrorists are busy with their business. Therefore, they have no time to carry out bomb attacks.

Keywords: Deradicalization, terrorists, entrepreneurship.

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Authors: Hojat Heidari-Bafroui, Amer Charbaji, Constantine Anagnostopoulos, Mohammad Faghri

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Phosphorus is an essential nutrient for plant life which is most frequently found as phosphate in water. Once phosphate is found in abundance in surface water, a series of adverse effects on an ecosystem can be initiated. Therefore, a portable and reliable method is needed to monitor the phosphate concentrations in the field. In this paper, an inexpensive dip strip device with the ascorbic acid/antimony reagent dried on blotting paper along with wet chemistry is developed for the detection of low concentrations of phosphate in water. Ammonium molybdate and sulfuric acid are separately stored in liquid form so as to improve significantly the lifetime of the device and enhance the reproducibility of the device’s performance. The limit of detection and quantification for the optimized device are 0.134 ppm and 0.472 ppm for phosphate in water, respectively. The device’s shelf life, storage conditions, and limit of detection are superior to what has been previously reported for the paper-based phosphate detection devices.

Keywords: Phosphate detection, paper-based device, molybdenum blue method, colorimetric assay.

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Authors: Ek Ngoy, A F Mulaba-Bafubiandi

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Cobalt was acid nitric leached from a mixed cobaltcopper oxide with variable acid concentration. Resulting experimental data were used to analyze effects of increase in acid concentration, based on a shrinking core model of the process. The mathematical simulation demonstrated that the time rate of the dissolution mechanism is an increasing function of acid concentration. It was also shown that the magnitude of the acid concentration effect is time dependent and the increase in acid concentration is more effective at earlier stage of the dissolution than at later stage. The remaining process parameters are comprehensively affected by acid concentration and their interaction is synergetic.

Keywords: Acid effect, Cobalt, Cobalt-copper oxide, Leaching, Simulation

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Authors: J. Dubois, P. Jreije

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Internet security attack could endanger the privacy of World Wide Web users and the integrity of their data. The attack can be carried out on today's most secure systems- browsers, including Netscape Navigator and Microsoft Internet Explorer. There are too many types, methods and mechanisms of attack where new attack techniques and exploits are constantly being developed and discovered. In this paper, various types of internet security attack mechanisms are explored and it is pointed out that when different types of attacks are combined together, network security can suffer disastrous consequences.

Keywords: DoS, internet attacks, router attack, security, trojan, virus, worm, XSS.

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Authors: Ayush Chugh, Gaurav Gupta

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Today’s technology is heavily dependent on web applications. Web applications are being accepted by users at a very rapid pace. These have made our work efficient. These include webmail, online retail sale, online gaming, wikis, departure and arrival of trains and flights and list is very long. These are developed in different languages like PHP, Python, C#, ASP.NET and many more by using scripts such as HTML and JavaScript. Attackers develop tools and techniques to exploit web applications and legitimate websites. This has led to rise of web application security; which can be broadly classified into Declarative Security and Program Security. The most common attacks on the applications are by SQL Injection and XSS which give access to unauthorized users who totally damage or destroy the system. This paper presents a detailed literature description and analysis on Web Application Security, examples of attacks and steps to mitigate the vulnerabilities.

Keywords: Attacks, Injection, JavaScript, SQL, Vulnerability, XSS.

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Authors: Ambika Vishal Pawar, Ajay Dani

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Considering the complexities involved in Cloud computing, there are still plenty of issues that affect the privacy of data in cloud environment. Unless these problems get solved, we think that the problem of preserving privacy in cloud databases is still open. In tokenization and homomorphic cryptography based solutions for privacy preserving cloud database querying, there is possibility that by colluding with service provider adversary may run brute force attacks that will reveal the attribute values.

In this paper we propose a solution by defining the variant of K –means clustering algorithm that effectively detects such brute force attacks and enhances privacy of cloud database querying by preventing this attacks.

Keywords: Privacy, Database, Cloud Computing, Clustering, K-means, Cryptography.

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Authors: Hoai-Vu Nguyen, Yongsun Choi

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Distributed denial-of-service (DDoS) attacks pose a serious threat to network security. There have been a lot of methodologies and tools devised to detect DDoS attacks and reduce the damage they cause. Still, most of the methods cannot simultaneously achieve (1) efficient detection with a small number of false alarms and (2) real-time transfer of packets. Here, we introduce a method for proactive detection of DDoS attacks, by classifying the network status, to be utilized in the detection stage of the proposed anti-DDoS framework. Initially, we analyse the DDoS architecture and obtain details of its phases. Then, we investigate the procedures of DDoS attacks and select variables based on these features. Finally, we apply the k-nearest neighbour (k-NN) method to classify the network status into each phase of DDoS attack. The simulation result showed that each phase of the attack scenario is classified well and we could detect DDoS attack in the early stage.

Keywords: distributed denial-of-service (DDoS), k-nearestneighbor classifier (k-NN), anti-DDoS framework, DDoS detection.

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Authors: Atitaya Singchai, Nooduan Muangsan, Thitiporn Machikowa

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Sunflower oil with high oleic acid content is most desirable because of its high oxidative stability. Screening sunflower of high oleic acid using conventional method is laborious and time consuming. Therefore, the use of molecular markers as a screening tool is promising. The objective of this research was to evaluate SSR primers for high oleic acid content in sunflower. Two sunflower lines, 5A and PI 649855 were used as the representative of low and high oleic acid sunflowers, respectively, and thirty seven SSR markers were used to identify oleic acid content trait. The results revealing 10 SSR primers showed polymorphic between high and low oleic acid lines and thus were informative. With these primers, therefore, it is possible to identify the genetic markers associated with high oleic acid trait in sunflower genotypes. 

Keywords: Microsatellite, Helianthus annuus L., fatty acid composition, molecular markers.

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Authors: Mouhammad Alkasassbeh, Mohammad Almseidin

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Network security engineers work to keep services available all the time by handling intruder attacks. Intrusion Detection System (IDS) is one of the obtainable mechanisms that is used to sense and classify any abnormal actions. Therefore, the IDS must be always up to date with the latest intruder attacks signatures to preserve confidentiality, integrity, and availability of the services. The speed of the IDS is a very important issue as well learning the new attacks. This research work illustrates how the Knowledge Discovery and Data Mining (or Knowledge Discovery in Databases) KDD dataset is very handy for testing and evaluating different Machine Learning Techniques. It mainly focuses on the KDD preprocess part in order to prepare a decent and fair experimental data set. The J48, MLP, and Bayes Network classifiers have been chosen for this study. It has been proven that the J48 classifier has achieved the highest accuracy rate for detecting and classifying all KDD dataset attacks, which are of type DOS, R2L, U2R, and PROBE.

Keywords: IDS, DDoS, MLP, KDD.

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Authors: Awad A. Younis, Elise Stronberg, Shifa Noor

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Users’ susceptibility to malware attacks have been noticed in the past few years. Investigating the factors that make a user vulnerable to those attacks is critical because they can be utilized to set up proactive strategies such as awareness and education to mitigate the impacts of those attacks. Demographic, behavioral, and cultural vulnerabilities are the main factors that make users susceptible to malware attacks. It is challenging, however, to draw more general conclusions based on those factors due to the varieties in the type of users and different types of malware. Therefore, we conducted a systematic literature review (SLR) of the existing research for user susceptibility factors to malware attacks. The results showed that all demographic factors are consistently associated with malware infection regardless of the users' type except for age and gender. Besides, the association of culture and personality factors with malware infection is consistent in most of the selected studies and for all types of users. Moreover, malware infection varies based on age, geographic location, and host types. We propose that future studies should carefully take into consideration the type of users because different users may be exposed to different threats or targeted based on their user domains’ characteristics. Additionally, as different types of malware use different tactics to trick users, taking the malware types into consideration is important.

Keywords: cybersecurity, malware, users, demographics, personality, culture, systematic literature review

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Authors: Halabi Hasbullah, Irshad Ahmed Soomro, Jamalul-lail Ab Manan

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Vehicular Ad-hoc Network (VANET) is taking more attention in automotive industry due to the safety concern of human lives on roads. Security is one of the safety aspects in VANET. To be secure, network availability must be obtained at all times since availability of the network is critically needed when a node sends any life critical information to other nodes. However, it can be expected that security attacks are likely to increase in the coming future due to more and more wireless applications being developed and deployed onto the well-known expose nature of the wireless medium. In this respect, the network availability is exposed to many types of attacks. In this paper, Denial of Service (DOS) attack on network availability is presented and its severity level in VANET environment is elaborated. A model to secure the VANET from the DOS attacks has been developed and some possible solutions to overcome the attacks have been discussed.

Keywords: Vehicular Ad hoc Network (VANET); security;availability; security attack; Denial of Service (DOS).

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Authors: Mingchuan Zhou, Haitao Zhang, Hongfang Ma, Weiyong Ying

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Alumina supported platinum and tin catalysts with different loadings of Pt and Sn were prepared and characterized by low temperature N₂ adsorption/desorption, H₂-temperature programed reduction and CO pulse chemisorption. Pt and Sn below 1% loading were suitable for acetic acid hydrogenation. The best performance over 0.75Pt1Sn/Al₂O₃ can reach 87.55% conversion of acetic acid and 47.39% selectivity of ethanol. The operating conditions of acetic acid hydrogenation over 1Pt1Sn/Al₂O₃ were investigated. High reaction temperature can enhance the conversion of acetic acid, but it decreased total selectivity of ethanol and acetyl acetate. High pressure and low weight hourly space velocity were beneficial to both conversion of acetic acid and selectivity to ethanol.

Keywords: Acetic acid, hydrogenation, PtSn, operating condition.

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Authors: Sunil Patel, Pallab Maji

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To study the effect of adversarial attack environment must be controlled. Autonomous driving includes mainly 5 phases sense, perceive, map, plan, and drive. Autonomous vehicles sense their surrounding with the help of different sensors like cameras, radars, and lidars. Deep learning techniques are considered Blackbox and found to be vulnerable to adversarial attacks. In this research, we study the effect of the various known adversarial attacks with the help of the Unreal Engine-based, high-fidelity, real-time raytraced simulated environment. The goal of this experiment is to find out if adversarial attacks work in moving vehicles and if an unknown network may be targeted. We discovered that the existing Blackbox and Whitebox attacks have varying effects on different traffic signs. We observed that attacks that impair detection in static scenarios do not have the same effect on moving vehicles. It was found that some adversarial attacks with hardly noticeable perturbations entirely blocked the recognition of certain traffic signs. We observed that the daylight condition has a substantial impact on the model's performance by simulating the interplay of light on traffic signs. Our findings have been found to closely resemble outcomes encountered in the real world.

Keywords: Adversarial attack simulation, computer simulation, ray-traced environment, realistic simulation, unreal engine.

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Authors: Rotchanaphan Hengaroonprasan, Malinee Sriariyanun, Prapakorn Tantayotai, Supacharee Roddecha, Kraipat Cheenkachorn

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Lignocellolusic material is a substance that is resistant to be degraded by microorganisms or hydrolysis enzymes. To be used as materials for biofuel production, it needs pretreatment process to improve efficiency of hydrolysis. In this work, chemical pretreatments on rice straw using three diluted organic acids, including acetic acid, citric acid, oxalic acid, were optimized. Using Response Surface Methodology (RSM), the effect of three pretreatment parameters, acid concentration, treatment time, and reaction temperature, on pretreatment efficiency were statistically evaluated. The results indicated that dilute oxalic acid pretreatment led to the highest enhancement of enzymatic saccharification by commercial cellulase and yielded sugar up to 10.67 mg/ml when using 5.04% oxalic acid at 137.11 oC for 30.01 min. Compared to other acid pretreatment by acetic acid, citric acid, and hydrochloric acid, the maximum sugar yields are 7.07, 6.30, and 8.53 mg/ml, respectively. Here, it was demonstrated that organic acids can be used for pretreatment of lignocellulosic materials to enhance of hydrolysis process, which could be integrated to other applications for various biorefinery processes. 

Keywords: Lignocellolusic biomass, pretreatment, organic acid response surface methodology, biorefinery.

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Authors: Sung-Bae Choi, Sang-Yoon Yoon, Eun-Jun Yoon

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In 2011, Debiao et al. pointed out that S-3PAKE protocol proposed by Lu and Cao for password-authenticated key exchange in the three-party setting is vulnerable to an off-line dictionary attack. Then, they proposed some countermeasures to eliminate the security vulnerability of the S-3PAKE. Nevertheless, this paper points out their enhanced S-3PAKE protocol is still vulnerable to undetectable on-line dictionary attacks unlike their claim.

Keywords: Authentication, 3PAKE, password, three-party key exchange, network security, dictionary attacks.

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Authors: Maros Barabas, Michal Drozd, Petr Hanacek

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A novel behavioral detection framework is proposed to detect zero day buffer overflow vulnerabilities (based on network behavioral signatures) using zero-day exploits, instead of the signature-based or anomaly-based detection solutions currently available for IDPS techniques. At first we present the detection model that uses shadow honeypot. Our system is used for the online processing of network attacks and generating a behavior detection profile. The detection profile represents the dataset of 112 types of metrics describing the exact behavior of malware in the network. In this paper we present the examples of generating behavioral signatures for two attacks – a buffer overflow exploit on FTP server and well known Conficker worm. We demonstrated the visualization of important aspects by showing the differences between valid behavior and the attacks. Based on these metrics we can detect attacks with a very high probability of success, the process of detection is however very expensive.

Keywords: behavioral signatures, metrics, network, security design

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Authors: Widi Astuti, Agus Prasetya, Endang Tri Wahyuni, I Made Bendiyasa

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Coal fly ash (CFA) generated by coal-based thermal power plants is mainly composed of quartz, mullite, and unburned carbon. In this study, the effect of unburned carbon on CFA toward its adsorption capacity was investigated. CFA with various carbon content was obtained by refluxing it with sulfuric acid having various concentration at various temperature and reflux time, by heating at 400-800°C, and by sieving into 100-mesh in particle size. To evaluate the effect of unburned carbon on CFA toward its adsorption capacity, adsorption of methyl violet solution with treated CFA was carried out. The research shows that unburned carbon leads to adsorption capacity decrease. The highest adsorption capacity of treated CFA was found 5.73 x 10-4mol.g-1.

Keywords: CFA, carbon, methyl violet, adsorption capacity.

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Authors: Woranart Jonglertjunya, Nattawadee Pranrawang, Nuanyai Phookongka, Thanasak Sridangtip, Watthana Sawedrungreang, Chularat Krongtaew

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Sugarcane bagasses are one of the most extensively used agricultural residues. Using acid hydrolysis and fermentation, conversion of sugarcane bagasses to lactic acid was technically and economically feasible. This research was concerned with the solubility of lignin in ammonium hydroxide, acid hydrolysis and lactic acid fermentation by Lactococcus lactis, Lactobacillus delbrueckii, Lactobacillus plantarum, and Lactobacillus casei. The lignin extraction results for different ammonium hydroxide concentrations showed that 10 % (v/v) NH4OH was favorable to lignin dissolution. Acid hydrolysis can be enhanced with increasing acid concentration and reaction temperature. The optimum glucose and xylose concentrations occurred at 121 ○C for 1 hour hydrolysis time in 10% sulphuric acid solution were 32 and 11 g/l, respectively. In order to investigate the significance of medium composition on lactic acid production, experiments were undertaken whereby a culture of Lactococcus lactis was grown under various glucose, peptone, yeast extract and xylose concentrations. The optimum medium was composed of 5 g/l glucose, 2.5 g/l xylose, 10 g/l peptone and 5 g/l yeast extract. Lactococcus lactis represents the most efficient for lactic acid production amongst those considered. The lactic acid fermentation by Lactococcus lactis after 72 hours gave the highest yield of 1.4 (g lactic acid per g reducing sugar).

Keywords: sugarcane bagasses, acid hydrolysis, lactic acid, fermentation

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Authors: Glauber Cruz, Patrícia A. S. Monteiro, Carlos E. M. Braz, Paulo Seleghin Jr., Igor Polikarpov, Paula M.Crnkovic

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Enzymatic hydrolysis is one of the major steps involved in the conversion from sugarcane bagasse to yield ethanol. This process offers potential for yields and selectivity higher, lower energy costs and milder operating conditions than chemical processes. However, the presence of some factors such as lignin content, crystallinity degree of the cellulose, and particle sizes, limits the digestibility of the cellulose present in the lignocellulosic biomasses. Pretreatment aims to improve the access of the enzyme to the substrate. In this study sugarcane bagasse was submitted chemical pretreatment that consisted of two consecutive steps, the first with dilute sulfuric acid (1 % (v/v) H2SO4), and the second with alkaline solutions with different concentrations of NaOH (1, 2, 3 and 4 % (w/v)). Thermal Analysis (TG/ DTG and DTA) was used to evaluate hemicellulose, cellulose and lignin contents in the samples. Scanning Electron Microscopy (SEM) was used to evaluate the morphological structures of the in natura and chemically treated samples. Results showed that pretreatments were effective in chemical degradation of lignocellulosic materials of the samples, and also was possible to observe the morphological changes occurring in the biomasses after pretreatments.

Keywords: Alkaline solutions, bioethanol production, dilute acid, enzymatic hydrolysis, lignocellulosic biomass.

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Authors: Sibel Uzuner, Jale Acar

Abstract:

Pomegranate and pomegranate juices (PJs) have taken great attention for their health benefits in the last years. As there is an increasing concern about potential health benefits of ellagic acid, it is of great interest to evaluate alterations in ellagic acid concentration of commercial PJs. The purpose of this study is to analyze total phenolic, free and total ellagic acid content of six commercial PJs sold in Turkish markets using HPLC. The results showed that some commercial PJs had markedly high total phenolic and ellagic acid content. Total phenolic substances of commercial PJs range from 796.71 to 4608.91 mg GAE/l. Free amount of ellagic acid in commercial PJs range from 27.64 to 111.78 mg/l. Samples are hydrolyzed with concentrated HCl at 93oC for 2 and 24 hour and influences of temperature and time parameters on hydrolization were investigated. Thermal processing for pasteurization increased ellagic acid via ellagitannins hydrolysis.

Keywords: Ellagic acid, ellagitannin, pomegranate juice, total phenolic compounds

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