{"title":"Optimization of Diluted Organic Acid Pretreatment on Rice Straw Using Response Surface Methodology ","authors":"Rotchanaphan Hengaroonprasan, Malinee Sriariyanun, Prapakorn Tantayotai, Supacharee Roddecha, Kraipat Cheenkachorn ","volume":101,"journal":"International Journal of Bioengineering and Life Sciences","pagesStart":512,"pagesEnd":517,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/10001300","abstract":"
Lignocellolusic material is a substance that is resistant to be degraded by microorganisms or hydrolysis enzymes. To be used as materials for biofuel production, it needs pretreatment process to improve efficiency of hydrolysis. In this work, chemical pretreatments on rice straw using three diluted organic acids, including acetic acid, citric acid, oxalic acid, were optimized. Using Response Surface Methodology (RSM), the effect of three pretreatment parameters, acid concentration, treatment time, and reaction temperature, on pretreatment efficiency were statistically evaluated. The results indicated that dilute oxalic acid pretreatment led to the highest enhancement of enzymatic saccharification by commercial cellulase and yielded sugar up to 10.67 mg\/ml when using 5.04% oxalic acid at 137.11 oC for 30.01 min. Compared to other acid pretreatment by acetic acid, citric acid, and hydrochloric acid, the maximum sugar yields are 7.07, 6.30, and 8.53 mg\/ml, respectively. Here, it was demonstrated that organic acids can be used for pretreatment of lignocellulosic materials to enhance of hydrolysis process, which could be integrated to other applications for various biorefinery processes. <\/p>\r\n","references":"[1] R. Sathre, \u201cComparing the heat of combustion of fossil fuels to the heat\r\naccumulated by their lifecycle greenhouse gases,\u201d in 2014 Fuel., vol.\r\n115, pp. 674-677.\r\n[2] V. Menon, M. Rao, \u201cTrends in bioconversion of lignocellulose:\r\nBiofuels, platform chemicals & biorefinery concept,\u201d in 2012 Prog\r\nEnergy Combust Sci., vol. 38, pp. 522-550.\r\n[3] R. E. Hage, L. Chrusciel, L. Desharnais, N. Brosse, \u201cEffect of\r\nautohydrolysis of Miscanthus \u00d7 giganteus on lignin structure and\r\norganosolv delignification,\u201d in 2010 Bioresour. Technol., vol. 101, pp.\r\n9321\u20139329.\r\n[4] G. Y. S. Mtui, \u201cRecent advances in pretreatment of lignocellulosic\r\nwastes and production of value added products\u201d in 2009 Afr J of\r\nbiotechnol., vol. 8 , pp. 1398-1415.\r\n[5] Y. Zheng1, J. Zhao, F. Xu, Y. Li, \u201cPretreatment of lignocellulosic\r\nbiomass for enhanced biogas production,\u201d in 2014 Prog Energy\r\nCombust Sci., vol. 42, pp. 35\u201353.\r\n[6] I. Kim, B. Lee, J. Park, S. Choi, J. Han, \u201cEffect of nitric acid on\r\npretreatment and fermentation for enhancing ethanol production of rice\r\nstraw\u201d in 2014 Carbohyd. Polym., vol. 99, pp. 563-567.\r\n[7] N. Gil, S. Ferreira, M. E. Amaral, F. C. Domingues, A. P. Duarte, \u201cThe\r\ninfluence of dilute acid pretreatment conditions on the enzymatic\r\nsaccharification of Erica spp. for bioethanol production\u201d in 2010 Ind.\r\nCrop Prod., vol. 32 pp. 29-35.\r\n[8] F. Monlau, A. Barakat1\r\n, J.P. Steyer, H. Carrere, \u201cComparison of seven\r\ntypes of thermo-chemical pretreatments on the structural features and\r\nanaerobic digestion of sunflower stalks\u201d in 2012 Bioresour. Technol.,\r\nvol. 120 pp. 241\u2013247. \r\n[9] A.T.W.M. Hendriks, 1, G. Zeeman, \u201cPretreatments to enhance the\r\ndigestibility of lignocellulosic biomass,\u201d in 2010 Bioresour. Technol.,\r\nvol. 100 pp. 10\u201318.\r\n[10] A. M. J. Kootstra, H. H. Beeftink, E. L. Scott, J. P.M. Sanders,\r\n\"Comparison of dilute mineral and organic acid pretreatment for\r\nenzymatic hydrolysis of wheat straw.\" in 2009 Biochem. Eng. J., vol. 46\r\npp. 126-131.\r\n[11] X. Zhao, L. Wangb, X. Lu, S. Zhang, \"Pretreatment of corn stover with\r\ndiluted acetic acid for enhancement of acidogenic fermentation.\" in 2014\r\nBioresour Technol., vol. 158, pp. 12-18.\r\n[12] V. Soest, P. J., J. B. Robertson, B. A. Lewis, \u201cMethods of dietary fibre,\r\nneutral detergent fibre, and non strach polysaccharides in relation to\r\nanimal nutrition\u201d. in 1991 J. Dairy Sci., vol. 74, pp.3583-3597.\r\n[13] AOAC International, \"Total dietary fiber in foods, enzymaticgravimetric\r\nmethod\". in \"Official methods of analysis of AOAC\r\ninternational\". in 2003, 17th ed. 985.29. Gaithersburg, MD, U.S.A.\r\n[14] G. L. Miller, \u201cUse of dinitro salicylic acid reagent for determination of\r\nreducing sugar,\u201d in 1959 Anal. Chem., vol. 31, no. 3, pp. 426\u2013428.","publisher":"World Academy of Science, Engineering and Technology","index":"Open Science Index 101, 2015"}