Search results for: stochastic optimal control technique
3003 Slice Bispectrogram Analysis-Based Classification of Environmental Sounds Using Convolutional Neural Network
Authors: Katsumi Hirata
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Certain systems can function well only if they recognize the sound environment as humans do. In this research, we focus on sound classification by adopting a convolutional neural network and aim to develop a method that automatically classifies various environmental sounds. Although the neural network is a powerful technique, the performance depends on the type of input data. Therefore, we propose an approach via a slice bispectrogram, which is a third-order spectrogram and is a slice version of the amplitude for the short-time bispectrum. This paper explains the slice bispectrogram and discusses the effectiveness of the derived method by evaluating the experimental results using the ESC‑50 sound dataset. As a result, the proposed scheme gives high accuracy and stability. Furthermore, some relationship between the accuracy and non-Gaussianity of sound signals was confirmed.
Keywords: Bispectrum, convolutional neural network, environmental sound, slice bispectrogram, spectrogram.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6183002 Trust In Ad Media
Authors: Duygu Aydin
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Advertising today has already become an integral part of human life as a building block of the consumer community. A component of the value chain of the media, advertising sector is struggling increasingly harder to find new methods to reach consumers. The tendency towards experimental marketing practices is increasing day by day, especially to divert consumers from the idea “They are selling something to me.” It is therefore considered a good idea to investigate the trust in ad media of consumers, who are today exposed to a great bulk of information from advertising sector. In this study, the current value of ad media for the young consumer will be investigated. Data on various ad media reliability will be comparatively analyzed and young consumers will be traced by including university students in the study. In this research, which will be performed on students studying at the Selçuk University (Turkey) by random sampling method, data will be obtained by survey technique and evaluated by a statistical analysis.Keywords: Trust in advertising, ad medium, media.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19483001 Development of Risk Assessment and Occupational Safety Management Model for Building Construction Projects
Authors: Preeda Sansakorn, Min An
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In order to be capable of dealing with uncertainties, subjectivities, including vagueness arising in building construction projects, the application of fuzzy reasoning technique based on fuzzy set theory is proposed. This study contributes significantly to the development of a fuzzy reasoning safety risk assessment model for building construction projects that could be employed to assess the risk magnitude of each hazardous event identified during construction, and a third parameter of probability of consequence is incorporated in the model. By using the proposed safety risk analysis methodology, more reliable and less ambiguities, which provide the safety risk management project team for decision-making purposes.
Keywords: Safety risks assessment, building construction safety, fuzzy reasoning, construction risk assessment model, building construction projects.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 23453000 Unsteady Water Boundary Layer Flow with Non-Uniform Mass Transfer
Authors: G. Revathi, P. Saikrishnan
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In the present analysis an unsteady laminar forced convection water boundary layer flow is considered. The fluid properties such as viscosity and Prandtl number are taken as variables such that those are inversely proportional to temperature. By using quasi-linearization technique the nonlinear coupled partial differential equations are linearized and the numerical solutions are obtained by using implicit finite difference scheme with the appropriate selection of step sizes. Non-similar solutions have been obtained from the starting point of the stream-wise coordinate to the point where skin friction value vanishes. The effect non-uniform mass transfer along the surface of the cylinder through slot is studied on the skin friction and heat transfer coefficients.Keywords: Boundary layer, heat transfer, non-similar solution, non-uniform mass, unsteady flow.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 19682999 Thermal Fracture Analysis of Fibrous Composites with Variable Fiber Spacing Using Jk-Integral
Authors: Farid Saeidi, Serkan Dag
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In this study, fracture analysis of a fibrous composite laminate with variable fiber spacing is carried out using Jk-integral method. The laminate is assumed to be under thermal loading. Jk-integral is formulated by using the constitutive relations of plane orthotropic thermoelasticity. Developed domain independent form of the Jk-integral is then integrated into the general purpose finite element analysis software ANSYS. Numerical results are generated so as to assess the influence of variable fiber spacing on mode I and II stress intensity factors, energy release rate, and T-stress. For verification, some of the results are compared to those obtained using displacement correlation technique (DCT).Keywords: Jk-integral, variable fiber spacing, thermoelasticity, t-stress, finite element method, fibrous composite.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10082998 Performance Analysis of Adaptive OFDM Pre and Post-FTT Beamforming System
Authors: S. Elnobi, Iman El-Zahaby, Amr M. Mahros
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In mobile communication systems, performance and capacity are affected by multi-path fading, delay spread and Co-Channel Interference (CCI). For this reason Orthogonal Frequency Division Multiplexing (OFDM) and adaptive antenna array are used is required. The goal of the OFDM is to improve the system performance against Inter-Symbol Interference (ISI). An array of adaptive antennas has been employed to suppress CCI by spatial technique. To suppress CCI in OFDM systems two main schemes the pre-FFT and the post-FFT have been proposed. In this paper, through a system level simulation, the behavior of the pre-FFT and post-FFT beamformers for OFDM system has been investigated based on two algorithms namely, Least Mean Squares (LMS) and Recursive Least Squares (RLS). The performance of the system is also discussed in multipath fading channel system specified by 3GPP Long Term Evolution (LTE).
Keywords: OFDM, Beamforming, Adaptive Antennas Array.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 24402997 Mobile Assembly of Electric Vehicles: Decentralized, Low-Invest and Flexible
Authors: Achim Kampker, Kai Kreiskoether, Johannes Wagner, Sarah Fluchs
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The growing speed of innovation in related industries requires the automotive industry to adapt and increase release frequencies of new vehicle derivatives which implies a significant reduction of investments per vehicle and ramp-up times. Emerging markets in various parts of the world augment the currently dominating established main automotive markets. Local content requirements such as import tariffs on final products impede the accessibility of these micro markets, which is why in the future market exploitation will not be driven by pure sales activities anymore but rather by setting up local assembly units. The aim of this paper is to provide an overview of the concept of decentralized assembly and to discuss and critically assess some currently researched and crucial approaches in production technology. In order to determine the scope in which complementary mobile assembly can be profitable for manufacturers, a general cost model is set up and each cost driver is assessed with respect to varying levels of decentralization. One main result of the paper is that the presented approaches offer huge cost-saving potentials and are thus critical for future production strategies. Nevertheless, they still need to be further exploited in order for decentralized assembly to be profitable for companies. The optimal level of decentralization must, however, be specifically determined in each case and cannot be defined in general.Keywords: Automotive assembly, e-mobility, production technology, small series assembly.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14732996 Development of Variable Stepsize Variable Order Block Method in Divided Difference Form for the Numerical Solution of Delay Differential Equations
Authors: Fuziyah Ishak, Mohamed B. Suleiman, Zanariah A. Majid, Khairil I. Othman
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This paper considers the development of a two-point predictor-corrector block method for solving delay differential equations. The formulae are represented in divided difference form and the algorithm is implemented in variable stepsize variable order technique. The block method produces two new values at a single integration step. Numerical results are compared with existing methods and it is evident that the block method performs very well. Stability regions of the block method are also investigated.Keywords: block method, delay differential equations, predictor-corrector, stability region, variable stepsize variable order.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14752995 Embodied Carbon Footprint of Existing Malaysian Green Homes
Authors: Fahanim Abdul Rashid, Muhammad Azzam Ismail
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Part and parcel of building green homes (GHs) with favorable thermal comfort (TC) is to design and build with reduced carbon footprint (CF) from embodied energy in the building envelope and reduced operational CF overall. Together, the environmental impact of GHs can be reduced significantly. Nevertheless, there is still a need to identify the base CF value for Malaysian GHs and this can be done by assessing existing ones which can then be compared to conventional and vernacular houses which are built differently with different building materials. This paper underlines the research design and introduces the case studies. For now, the operational CF of the case studies is beyond the scope of this study. Findings from this research could identify the best building material and construction technique combination to build GHs depending on the available skills, financial constraints and the condition of the immediate environment.
Keywords: Embodied carbon footprint, Malaysian green homes.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 21982994 An Efficient Run Time Interface for Heterogeneous Architecture of Large Scale Supercomputing System
Authors: Prabu D., Andrew Aaron James, Vanamala V., Vineeth Simon, Sanjeeb Kumar Deka, Sridharan R., Prahlada Rao B.B., Mohanram N.
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In this paper we propose a novel Run Time Interface (RTI) technique to provide an efficient environment for MPI jobs on the heterogeneous architecture of PARAM Padma. It suggests an innovative, unified framework for the job management interface system in parallel and distributed computing. This approach employs proxy scheme. The implementation shows that the proposed RTI is highly scalable and stable. Moreover RTI provides the storage access for the MPI jobs in various operating system platforms and improve the data access performance through high performance C-DAC Parallel File System (C-PFS). The performance of the RTI is evaluated by using the standard HPC benchmark suites and the simulation results show that the proposed RTI gives good performance on large scale supercomputing system.Keywords: RTI, C-MPI, C-PFS, Scheduler Interface.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14412993 Ultra-Precise Hybrid Lens Distortion Correction
Authors: Christian Bräuer-Burchardt, Peter Kühmstedt, Gunther Notni
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A new hybrid method to realise high-precision distortion determination for optical ultra-precision 3D measurement systems based on stereo cameras using active light projection is introduced. It consists of two phases: the basic distortion determination and the refinement. The refinement phase of the procedure uses a plane surface and projected fringe patterns as calibration tools to determine simultaneously the distortion of both cameras within an iterative procedure. The new technique may be performed in the state of the device “ready for measurement" which avoids errors by a later adjustment. A considerable reduction of distortion errors is achieved and leads to considerable improvements of the accuracy of 3D measurements, especially in the precise measurement of smooth surfaces.Keywords: 3D Surface Measurement, Fringe Projection, Lens Distortion, Stereo.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 20672992 Development of a New CFD Multi-Coupling Tool Based on Immersed Boundary Method: toward SRM Analysis
Authors: Ho Phu TRAN, Frédéric PLOURDE
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The ongoing effort to develop an in-house compressible solver with multi-disciplinary physics is presented in this paper. Basic compressible solver combined with IBM technique provides us an effective numerical tool able to tackle the physics phenomena and especially physic phenomena involved in Solid Rocket Motors (SRMs). Main principles are introduced step by step describing its implementation. This paper sheds light on the whole potentiality of our proposed numerical model and we strongly believe a way to introduce multi-physics mechanisms strongly coupled is opened to ablation in nozzle, fluid/structure interaction and burning propellant surface with time.Keywords: Compressible Flow, Immersed Boundary Method, Multi-disciplinary physics, Solid Rocket Motors.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 18382991 Routing Medical Images with Tabu Search and Simulated Annealing: A Study on Quality of Service
Authors: Mejía M. Paula, Ramírez L. Leonardo, Puerta A. Gabriel
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In telemedicine, the image repository service is important to increase the accuracy of diagnostic support of medical personnel. This study makes comparison between two routing algorithms regarding the quality of service (QoS), to be able to analyze the optimal performance at the time of loading and/or downloading of medical images. This study focused on comparing the performance of Tabu Search with other heuristic and metaheuristic algorithms that improve QoS in telemedicine services in Colombia. For this, Tabu Search and Simulated Annealing heuristic algorithms are chosen for their high usability in this type of applications; the QoS is measured taking into account the following metrics: Delay, Throughput, Jitter and Latency. In addition, routing tests were carried out on ten images in digital image and communication in medicine (DICOM) format of 40 MB. These tests were carried out for ten minutes with different traffic conditions, reaching a total of 25 tests, from a server of Universidad Militar Nueva Granada (UMNG) in Bogotá-Colombia to a remote user in Universidad de Santiago de Chile (USACH) - Chile. The results show that Tabu search presents a better QoS performance compared to Simulated Annealing, managing to optimize the routing of medical images, a basic requirement to offer diagnostic images services in telemedicine.
Keywords: Medical image, QoS, simulated annealing, Tabu search, telemedicine.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 9562990 Adverse Curing Conditions and Performance of Concrete: Bangladesh Perspective
Authors: T. Manzur
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Concrete is the predominant construction material in Bangladesh. In large projects, stringent quality control procedures are usually followed under the supervision of experienced engineers and skilled labors. However, in the case of small projects and particularly at distant locations from major cities, proper quality control is often an issue. It has been found from experience that such quality related issues mainly arise from inappropriate proportioning of concrete mixes and improper curing conditions. In most cases external curing method is followed which requires supply of adequate quantity of water along with proper protection against evaporation. Often these conditions are found missing in the general construction sites and eventually lead to production of weaker concrete both in terms of strength and durability. In this study, an attempt has been made to investigate the performance of general concreting works of the country when subjected to several adverse curing conditions that are quite common in various small to medium construction sites. A total of six different types of adverse curing conditions were simulated in the laboratory and samples were kept under those conditions for several days. A set of samples was also submerged in normal curing condition having proper supply of curing water. Performance of concrete was evaluated in terms of compressive strength, tensile strength, chloride permeability and drying shrinkage. About 37% and 25% reduction in 28-day compressive and tensile strength were observed respectively, for samples subjected to most adverse curing condition as compared to the samples under normal curing conditions. Normal curing concrete exhibited moderate permeability (close to low permeability) whereas concrete under adverse curing conditions showed very high permeability values. Similar results were also obtained for shrinkage tests. This study, thus, will assist concerned engineers and supervisors to understand the importance of quality assurance during the curing period of concrete.
Keywords: Adverse, concrete, curing, compressive strength, drying shrinkage, permeability, tensile strength.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 10622989 Real E-Government, Real Convenience
Authors: M. Kargar, F.Fartash, T. Saderi, M. Abdar-e Bakhshayesh
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In this paper we have suggested a new system for egovernment. In this method a government can design a precise and perfect system to control people and organizations by using five major documents. These documents contain the important information of each member of a society and help all organizations to do their informatics tasks through them. This information would be available by only a national code and a secure program would support it. The suggested system can give a good awareness to the society and help it be managed correctly.Keywords: E-Government, Internet, Web-Based System, Society.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15052988 A Mark-Up Approach to Add Value
Authors: Ivaylo I. Atanasov, Evelina N.Pencheva
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This paper presents a mark-up approach to service creation in Next Generation Networks. The approach allows deriving added value from network functions exposed by Parlay/OSA (Open Service Access) interfaces. With OSA interfaces service logic scripts might be executed both on callrelated and call-unrelated events. To illustrate the approach XMLbased language constructions for data and method definitions, flow control, time measuring and supervision and database access are given and an example of OSA application is considered.
Keywords: Service creation, mark-up approach.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16872987 Dynamic Analysis by a Family of Time Marching Procedures Based On Numerically Computed Green’s Functions
Authors: Delfim Soares Jr.
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In this work, a new family of time marching procedures based on Green’s function matrices is presented. The formulation is based on the development of new recurrence relationships, which employ time integral terms to treat initial condition values. These integral terms are numerically evaluated taking into account Newton-Cotes formulas. The Green’s matrices of the model are also numerically computed, taking into account the generalized-α method and subcycling techniques. As it is discussed and illustrated along the text, the proposed procedure is efficient and accurate, providing a very attractive time marching technique.
Keywords: Dynamics, Time-Marching, Green’s Function, Generalized-α Method, Subcycling.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 15152986 Easy Shopping by Electronic Credit
Authors: M. Kargar, A. Isazadeh, F. Fartash, T. Saderi
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In this paper we suggest a method for setting electronic credits for the customers. In this method banks and market-sites help each other to make doing large shopping through internet so easy. By developing this system, the people who have less money to buy most of the things they want, become able to buy all of them just through a credit. This credit is given by market-sites through a banking control on it. The method suggested can stop being imprisoned because of banking debts.Keywords: E-Business, E-Credit, Market-site, Buy-site, Bank, E-Commerce.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14862985 Multi-Font Farsi/Arabic Isolated Character Recognition Using Chain Codes
Authors: H. Izakian, S. A. Monadjemi, B. Tork Ladani, K. Zamanifar
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Nowadays, OCR systems have got several applications and are increasingly employed in daily life. Much research has been done regarding the identification of Latin, Japanese, and Chinese characters. However, very little investigation has been performed regarding Farsi/Arabic characters recognition. Probably the reason is difficulty and complexity of those characters identification compared to the others and limitation of IT activities in Farsi and Arabic speaking countries. In this paper, a technique has been employed to identify isolated Farsi/Arabic characters. A chain code based algorithm along with other significant peculiarities such as number and location of dots and auxiliary parts, and the number of holes existing in the isolated character has been used in this study to identify Farsi/Arabic characters. Experimental results show the relatively high accuracy of the method developed when it is tested on several standard Farsi fonts.Keywords: Farsi characters, OCR, feature extraction, chain code.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 23922984 The Role of Object Oriented Simulation F Modeling in Maintenance Processes
Authors: Abdulsalam A. Al-Sudairi
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Object-oriented simulation is considered one of the most sophisticated techniques that has been widely used in planning, designing, executing and maintaining construction projects. This technique enables the modeler to focus on objects which is extremely important for thorough understanding of a system. Thus, identifying an object is an essential point of building a successful simulation model. In a maintenance process an object is a maintenance work order (MWO). This study demonstrates a maintenance simulation model for the building maintenance division of Saudi Consolidated Electric Company (SCECO) in Dammam, Saudi Arabia. The model focused on both types of maintenance processes namely: (1) preventive maintenance (PM) and (2) corrective maintenance (CM). It is apparent from the findings that object-oriented simulation is a good diagnostic and experimental tool. This is because problems, limitations, bottlenecks and so forth are easily identified. These features are very difficult to obtain when using other tools.
Keywords: Object oriented, simulation, maintenance, process, work orders
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14972983 3D Finite Element Analysis for Mechanics of Soil-Tool Interaction
Authors: A. Armin, R. Fotouhi, W. Szyszkowski
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This paper is part of a study to develop robots for farming. As such power requirement to operate equipment attach to such robots become an important factor. Soil-tool interaction plays major role in power consumption, thus predicting accurately the forces which act on the blade during the farming is very important for optimal designing of farm equipment. In this paper, a finite element investigation for tillage tools and soil interaction is described by using an inelastic constitutive material law for agriculture application. A 3-dimensional (3D) nonlinear finite element analysis (FEA) is developed to examine behavior of a blade with different rake angles moving in a block of soil, and to estimate the blade force. The soil model considered is an elastic-plastic with non-associated Drucker-Prager material model. Special use of contact elements are employed to consider connection between soil-blade and soil-soil surfaces. The FEA results are compared with experimental ones, which show good agreement in accurately predicting draft forces developed on the blade when it moves through the soil. Also a very good correlation was obtained between FEA results and analytical results from classical soil mechanics theories for straight blades. These comparisons verified the FEA model developed. For analyzing complicated soil-tool interactions and for optimum design of blades, this method will be useful.Keywords: Finite element analysis, soil-blade contact modeling, blade force.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 30022982 Heterophase Polymerization of Pyrrole and Thienyl End Capped Ethoxylated Nonyl Phenol by Iron (III) Chloride
Authors: Görkem Ülkü, Esin A. Güvel, Nesrin Köken, Nilgün Kızılcan
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This study presents synthesis of novel block copolymers of thienyl end capped ethoxylated nonyl phenol and pyrrole via chemical oxidative polymerization. Ethoxylated nonyl phenol (ENP) was reacted with 2-thiophenecarbonyl chloride in order to synthesize a macromonomer containing thienyl end-group (ENPThC). Then copolymers of ENP-ThC and pyrrole were synthesized by chemical oxidative polymerization using iron (III) chloride as an oxidant. ENP-ThC served both as a macromonomer and an emulsifier for pyrrole with poor solubility in water. The synthesized block copolymers (ENP-ThC-b-PPy) were characterized by spectroscopic analysis and the electrical conductivities were investigated with 4-point probe technique.
Keywords: End capped polymer, ethoxylated nonyl phenol, heterophase polymerization, polypyrrole.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 25272981 Fruit Growing in Romania and Its Role for Rural Communities’ Development
Authors: Maria Toader, Gheorghe Valentin Roman
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The importance of fruit trees and bushes growing for Romania is due the concordance that exists between the different ecological conditions in natural basins, and the requirements of different species and varieties. There are, in Romania, natural areas dedicated to the main trees species: plum, apple, pear, cherry, sour cherry, finding optimal conditions for harnessing the potential of fruitfulness, making fruit quality both in terms of ratio commercial, and content in active principles. The share of fruits crops in the world economy of agricultural production is due primarily to the role of fruits in nourishment for human, and in the prevention and combating of diseases, in increasing the national income of cultivator countries and to improve comfort for human life. For Romania, the perspectives of the sector are positive, and are due to European funding opportunities, which provide farmers a specialized program that meets the needs of development and modernization of fruit growing industry, cultivation technology and equipment, organization and grouping of producers, creating storage facilities, conditioning, marketing and the joint use of fresh fruit. This paper shows the evolution of fruit growing, in Romania compared to other states. The document presents the current situation of the main tree species both in terms of surface but also of the productions and the role that this activity may have for the development of rural communities.
Keywords: Fruit growing, fruits trees, productivity, rural development.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 13882980 Three-Phase High Frequency AC Conversion Circuit with Dual Mode PWM/PDM Control Strategy for High Power IH Applications
Authors: Nabil A. Ahmed
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This paper presents a novel three-phase utility frequency to high frequency soft switching power conversion circuit with dual mode pulse width modulation and pulse density modulation for high power induction heating applications as melting of steel and non ferrous metals, annealing of metals, surface hardening of steel and cast iron work pieces and hot water producers, steamers and super heated steamers. This high frequency power conversion circuit can operate from three-phase systems to produce high current for high power induction heating applications under the principles of ZVS and it can regulate its ac output power from the rated value to a low power level. A dual mode modulation control scheme based on high frequency PWM in synchronization with the utility frequency positive and negative half cycles for the proposed high frequency conversion circuit and utility frequency pulse density modulation is produced to extend its soft switching operating range for wide ac output power regulation. A dual packs heat exchanger assembly is designed to be used in consumer and industrial fluid pipeline systems and it is proved to be suitable for the hot water, steam and super heated steam producers. Experiment and simulation results are given in this paper to verify the operation principles of the proposed ac conversion circuit and to evaluate its power regulation and conversion efficiency. Also, the paper presents a mutual coupling model of the induction heating load instead of equivalent transformer circuit model.Keywords: Induction heating, three-phase, conversion circuit, pulse width modulation, pulse density modulation, high frequency, soft switching.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 21782979 Automated Particle Picking based on Correlation Peak Shape Analysis and Iterative Classification
Authors: Hrabe Thomas, Beck Florian, Nickell Stephan
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Cryo-electron microscopy (CEM) in combination with single particle analysis (SPA) is a widely used technique for elucidating structural details of macromolecular assemblies at closeto- atomic resolutions. However, development of automated software for SPA processing is still vital since thousands to millions of individual particle images need to be processed. Here, we present our workflow for automated particle picking. Our approach integrates peak shape analysis to the classical correlation and an iterative approach to separate macromolecules and background by classification. This particle selection workflow furthermore provides a robust means for SPA with little user interaction. Processing simulated and experimental data assesses performance of the presented tools.Keywords: Cryo-electron Microscopy, Single Particle Analysis, Image Processing.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16682978 ATR-IR Study of the Mechanism of Aluminum Chloride Induced Alzheimer’s Disease; Curative and Protective Effect of Lipidium sativum Water Extract on Hippocampus Rats Brain Tissue
Authors: Maha Jameal Balgoon, Gehan A. Raouf, Safaa Y. Qusti, Soad Shaker Ali
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The main cause of Alzheimer disease (AD) was believed to be mainly due to the accumulation of free radicals owing to oxidative stress (OS) in brain tissue. The mechanism of the neurotoxicity of Aluminum chloride (AlCl3) induced AD in hippocampus Albino wister rat brain tissue, the curative & the protective effects of Lipidium sativum group (LS) water extract were assessed after 8 weeks by attenuated total reflection spectroscopy ATR-IR and histologically by light microscope. ATR-IR results revealed that the membrane phospholipid undergo free radical attacks, mediated by AlCl3, primary affects the polyunsaturated fatty acids indicated by the increased of the olefinic -C=CH sub-band area around 3012 cm-1 from the curve fitting analysis. The narrowing in the half band width (HBW) of the sνCH2 sub-band around 2852 cm-1 due to Al intoxication indicates the presence of trans form fatty acids rather than gauch rotomer. The degradation of hydrocarbon chain to shorter chain length, increasing in membrane fluidity, disorder, and decreasing in lipid polarity in AlCl3 group indicated by the detected changes in certain calculated area ratios compared to the control. Administration of LS was greatly improved these parameters compared to the AlCl3 group. Al influences the Aβ aggregation and plaque formation, which in turn interferes to and disrupts the membrane structure. The results also showed a marked increase in the β-parallel and antiparallel structure, that characterize the Aβ formation in Al-induced AD hippocampal brain tissue, indicated by the detected increase in both amide I sub-bands around 1674, 1692 cm-1. This drastic increase in Aβ formation was greatly reduced in the curative and protective groups compared to the AlCl3 group and approached nearly the control values. These results supported too by the light microscope. AlCl3 group showed significant marked degenerative changes in hippocampal neurons. Most cells appeared small, shrieked and deformed. Interestingly, the administration of LS in curative and protective groups markedly decreases the amount of degenerated cells compared to the non-treated group. In addition, the intensity of congo red stained cells was decreased. Hippocampal neurons looked more/or less similar to those of control. This study showed a promising therapeutic effect of Lipidium sativum group (LS) on AD rat model that seriously overcome the signs of oxidative stress on membrane lipid and restore the protein misfolding.Keywords: Aluminum chloride, Alzheimer’s disease, ATR-IR, Lipidium sativum.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 28102977 Heritage Tree Expert Assessment and Classification: Malaysian Perspective
Authors: B.-Y.-S. Lau, Y.-C.-T. Jonathan, M.-S. Alias
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Heritage trees are natural large, individual trees with exceptionally value due to association with age or event or distinguished people. In Malaysia, there is an abundance of tropical heritage trees throughout the country. It is essential to set up a repository of heritage trees to prevent valuable trees from being cut down. In this cross domain study, a web-based online expert system namely the Heritage Tree Expert Assessment and Classification (HTEAC) is developed and deployed for public to nominate potential heritage trees. Based on the nomination, tree care experts or arborists would evaluate and verify the nominated trees as heritage trees. The expert system automatically rates the approved heritage trees according to pre-defined grades via Delphi technique. Features and usability test of the expert system are presented. Preliminary result is promising for the system to be used as a full scale public system.Keywords: Arboriculture, Delphi, expert system, heritage tree, urban forestry.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 14322976 Extended Release System of Hypoglycemic Agent Containing Solid Dispersions: Strategies and Mechanisms
Authors: Amit Kumar, Ramendeep Grawal, Peeyush Sharma, Dinesh Puri, Anil Bhandari
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The main perspective of the present study aims at overcoming solubility problems by using the technique of solid dispersion. Repaglinide is a BCS Class II drug, having low aqueous solubility and therefore, low bioavailability. Solid dispersions of repaglinide with different carriers Polyvinyl Pyrrolidone (PVP) and Ethyl Cellulose (EC) in different ratios were prepared by suspending method and Dissolving methods. In vitro release studies revealed that the F7 formulation showed extended drug release. So, the dissolution profile of solid dispersion containing EC and PVP K30 (1: 3) was selected as the best formulation because of its extended drug release among all formulations. In conclusion, solid dispersions of Repaglinide in PVP have shown to be a promising approach to improve the bioavailability of Repaglinide.
Keywords: Ethyl Cellulose, Glibenclamide, Polyvinyl Pyrrolidone, Repaglinide, Solid Dispersion.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 26422975 Control of Staphylococcus aureus in Meat System by in situ and ex situ Bacteriocins from Lactobacillus sakei and Pediococcus spp.
Authors: M. Naimi, M. B. Khaled
Abstract:
The present study consisted of an applied test in meat system to assess the effectiveness of three bio agents bacteriocinproducing strains: Lm24: Lactobacillus sakei, Lm14and Lm25: Pediococcus spp. Two tests were carried out: The ex situ test was intended for three batches added with crude bacteriocin solutions at 12.48 AU/ml for Lm25 and 8.4 AU/ml for Lm14 and Lm24. However, the in situ one consisted of four batches; three of them inoculated with one bacteriocinogenic Lm25, Lm14, Lm24, respectively. The fourth one was used in mixture: Lm14+m24 at approximately of 107 CFU/ml. The two used tests were done in the presence of the pathogen St. aureus ATCC 6538, as a test strain at 103 CFU/ml. Another batch served as a positive or a negative control was used too. The incubation was performed at 7°C. Total viable counts, staphylococci and lactic acid bacteria, at the beginning and at selected times with interval of three days were enumerated. Physico-chemical determinations (except for in situ test): pH, dry mater, sugars, fat and total protein, at the beginning and at end of the experiment, were done, according to the international norms. Our results confirmed the ex situ effectiveness. Furthermore, the batches affected negatively the total microbial load over the incubation days, and showed a significant regression in staphylococcal load at day seven, for Lm14, Lm24, and Lm25 of 0.73, 2.11, and 2.4 log units. It should be noticed that, at the last day of culture, staphylococcal load was nil for the three batches. In the in situ test, the cultures displayed less inhibitory attitude and recorded a decrease in staphylococcal load, for Lm14, Lm24, Lm25, Lm14+m24 of 0.73, 0.20, 0.86, 0.032 log units. Therefore, physicochemical analysis for Lm14, Lm24, Lm25, Lm14+m24 showed an increase in pH from 5.50 to 5.77, 6.18, 5.96, 7.22, a decrease in dry mater from 7.30% to 7.05%, 6.87%, 6.32%, 6.00%.This result reflects the decrease in fat ranging from 1.53% to 1.49%, 1.07%, 0.99%, 0.87%; and total protein from 6.18% to 5.25%, 5.56%, 5.37%, 5.5%. This study suggests that the use of selected strains as Lm25 could lead to the best results and would help in preserving and extending the shelf life of lamb meat.
Keywords: Biocontrol, in situ and ex situ, meat system, St. aureus, Lactobacillus sakei, Pediococcus spp.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 16312974 Object Recognition Approach Based on Generalized Hough Transform and Color Distribution Serving in Generating Arabic Sentences
Authors: Nada Farhani, Naim Terbeh, Mounir Zrigui
Abstract:
The recognition of the objects contained in images has always presented a challenge in the field of research because of several difficulties that the researcher can envisage because of the variability of shape, position, contrast of objects, etc. In this paper, we will be interested in the recognition of objects. The classical Hough Transform (HT) presented a tool for detecting straight line segments in images. The technique of HT has been generalized (GHT) for the detection of arbitrary forms. With GHT, the forms sought are not necessarily defined analytically but rather by a particular silhouette. For more precision, we proposed to combine the results from the GHT with the results from a calculation of similarity between the histograms and the spatiograms of the images. The main purpose of our work is to use the concepts from recognition to generate sentences in Arabic that summarize the content of the image.
Keywords: Recognition of shape, generalized hough transformation, histogram, Spatiogram, learning.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 617